“It’s time to fight” for the future of Louisiana shrimpers and fishers The chefs and owners of Carmo, Dana Honn and Christina do Carmo Honn, announced this week they are closing their popular tropical restaurant at the end of 2024 after 15 years in the Warehouse District. But for these longtime leaders of climate-conscious cooking in New Orleans the end of Carmo marks the beginning of a new chapter involving three separate projects that prioritize sustainability Carmo’s last night will be December 21, Honn says, calling the closing “a little bit in the stars.” Since the onset of the pandemic, trends in the dining industry including increasing the cost of food, labor, and utilities have coincided with an even bigger factor: rent increases prompted by citywide insurance costs and property tax rate hikes “We haven’t really been profitable since COVID, so we’ve been examining how to transition for the last few years. We can’t change the size of our building, our location, or the number of employees, so we have to change course altogether,” says Honn. That new course includes three separate but related ventures: Nikkei, a Creole izakaya opening soon in the Broadside in Mid-City; Eatwell Kitchen a newly opened fast-casual restaurant in Metairie; and Tempero’s Market Kitchen a pop-up and events catering business that will revive several Carmo favorites Honn says the menu at Nikkei is a mix of traditional Japanese dishes like chashu pork a version of shrimp tempura found in Japan but also as popular street food in Liberdade a street dish of Japanese-Brazilian origin “You find pastel carts all over Brazil,” says Honn “But I’m not sure how well-known it is that Japanese immigrants created it.” One common thread throughout the menu is “localizing” the dishes through the Honns’ use of Gulf seafood and local produce. After all, it’s what they’re known for. In a report from Eater and Civil Eats author Grey Moran wrote about Carmo’s sustainability efforts: “Building the knowledge and relationships necessary for ethical regenerative sourcing has been a lifelong project for [Carmo’s] restaurant’s co-owners and chefs They’ve forged relationships with Gulf Coast shrimpers and Indigenous tribes in the Amazon to support traditional Honn says that when he and Christina originally started Carmo “there were not that many local producers to work with.” Fifteen years later and locality will be a focus of the operations at Nikkei Honn expects Nikkei to open the third week of November It seats about 25 inside and another 20 outside but the menu is designed for that kitchen,” Honn says and quick bites like potato and cheese fritters and white bean hummus Eatwell is open Monday through Friday from 10:30 a.m which also has a little known history in the city “These dishes represent the underrepresented It’s an ambitious new chapter — three new chapters “Things get busy during times of transition but I feel like at the end of this path there’s something better for our team members He recognizes his fellow chefs committed to working with local harvesters and suppliers — Susan Spicer of Bayona and Rosedale to name a few: “We have so many chefs here that walk the walk,” he says “I don’t think it’s quite that good everywhere Oops. 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All rights reserved (ANS – Dili) – “I’m in love with Timor!” Those heartfelt words from Pope Francis still echo in the ears of Salesian Cardinal Virgilio do Carmo da Silva The Holy Father spoke them just over seven months ago during the longest journey of his pontificate to Oceania and the Far East Cardinal da Silva recalled this moment—and the Pope’s deep affection for East Timor—during an interview with local journalists at Dili Airport on Tuesday as he prepared to embark on a 12,500 km journey to Rome to attend the funeral of the late Holy Father The Salesian Cardinal was already well aware of the Pope’s love for his country When he was appointed cardinal in May 2022 he remarked: “I’m convinced that Pope Francis didn’t offer this to me but to the Church and the people of East Timor.” And when he traveled to Rome on May 7 he stated during the ceremony at his titular church Albert the Great in northern Rome: “Making me a cardinal was a gift from the Pope to the youth and people of Timor.” That appointment made him not only one of the youngest members of the College of Cardinals at the time (a distinction he still holds at age 57) but also the first cardinal ever from East Timor—one of only two majority-Catholic countries in Asia Roughly 97% of East Timor’s population identifies as Catholic and during Pope Francis’ visit in September an estimated 600,000 people—nearly half the national population of 1.3 million—lined the streets to welcome the Vicar of Christ Cardinal do Carmo shared how he received the news of the Pope’s death He was preparing to celebrate a Mass in remembrance of the late pontiff when he was called to Rome for the funeral he now plays a role in the upcoming Conclave Cardinal do Carmo represents the kind of Church presence that Pope Francis often championed—one that lives and breathes in the “peripheries of the world,” a theme also echoed by Cardinal Giovanni Battista Re during his homily at the papal funeral on Saturday Cardinal do Carmo emphasized that he’s not heading to Rome with any specific papal candidate in mind: “We need to make a good discernment I’m happy to be able to participate.” Commenting on media speculation about whether the next pope should be "conservative" or "progressive," the Salesian Archbishop of Dili offered a thoughtful response: “I think what we need isn’t a pope who leans one way or the other but someone who remains neutral so that the Church can move in the right direction That’s my view—others may think differently But I believe balance is what’s needed.” ANS - “Agenzia iNfo Salesiana” is a on-line almost daily publication the communication agency of the Salesian Congregation enrolled in the Press Register of the Tibunal of Rome as n 153/2007 This site also uses third-party cookies to improve user experience and for statistical purposes By scrolling through this page or by clicking on any of its elements Dilson Do Carmo claimed the first of 10 $1 million prizes in the Massachusetts State Lottery’s “$4,000,000 Bonus Money” scratch ticket game He opted for a one-time prize payment of $650,000 will receive a $10,000 bonus for selling the winning ticket the first East Timorese cardinal is a Salesian from a small Catholic country where young people make up 70 per cent of the population marked by a long struggle for independence from Indonesia he inaugurated the John Paul II Catholic University and promotes an “open” and united Church He remembers the joy of welcoming the pontiff before an immense crowd on Francis’s trip last September Rome (AsiaNews) – “I am convinced that Pope Francis did not offer this to me but rather to the Church and the people of Timor-Leste,” said in 2022 Archbishop Virgilio do Carmo da Silva of Dili when he was elevated to the cardinalate by the late pontiff who picked most of the current members of the College of Cardinals This made him the first cardinal from the small Southeast Asian country with a Catholic majority (about 97 per cent) which the late pope visited during his last apostolic journey celebrating Mass in the Esplanade of Taci Tolu before a huge crowd (according to some estimates as many as 600,000 half of the small country’s population) The 57-year-old is certainly a face who embodies that "most peripheral periphery of the world" so dear to Francis which Cardinal Giovanni Battista Re mentioned during the homily in Saturday's funeral Mass Virgílio do Carmo da Silva was born in 1967 in Venilale He pursued his early education with the Salesians then entered the Salesian Society of Don Bosco The care the great Italian educator saint showed for young people inspired the magisterium of the East Timorese cardinal pastor in a country where about 70 per cent of the population is under 30 When he went to Rome on 7 May 2022 to receive his scarlet biretta during the celebration at the parish of St Albert the Great "Making me a cardinal was a gift from the pope to the youth and people of Timor,” he said on that occasion On numerous occasions he stated that he cares about young people aware of the strong push many feel to emigrate abroad in search of a more dignified life than in their country The parents of today’s young generation played a central role in the country's struggle for independence achieved only in 2002 after centuries of Portuguese colonial rule during which missionaries brought Christianity and many suffered martyrdom and Indonesia’s invasion and annexation in 1976 which saw the loss of thousands of lives in a long and brutal war who is the metropolitan archbishop of Dili together with the difficult post-war years marked by internal divisions among the country’s pro-independence forces Sent to Manila to study Philosophy and Theology he made his perpetual profession with the Salesians in 1997 and was ordained a priest the following year he obtained a licentiate in Spirituality at the Pontifical Salesian University in Rome He subsequently carried out his ministry back home and was appointed Provincial of the Salesians of his province in 2015 Pope Francis picked him in 2016 to be the bishop of Dili and then elevated the local Church to the rank of archdiocese three years later Carmo da Silva declared himself deeply "in love" with his country and its history His pastoral service has been devoted above all to education which is a priority in the social milieu in which he operates an example of his work is the inauguration of the John Paul II Catholic University in Dili The motto chosen for Pope Francis's visit last year was "May your faith become your culture" a commitment da Silva welcomed and stressed several times eager to help people "grow in faith” on the path towards unity hoping for an "open church" without hostile groups Francis's trip to Timor-Leste – 35 years after John Paul II’s undertaken when the country was still an Indonesian province – was a central experience in his pastoral ministry the Church and the entire people have contributed to the success of this visit,”  Silvia said the day after the visit “The strength of faith present in this young country has moved the Pope and almost the whole world." Mindful of the country's recent painful and troubled history struggling for independence the cardinal also said that Pope Francis’s visit had served to “affirm and confirm the national identity and to tell the Timorese that no matter how small the country is: you are Timorese and you are Catholic and you have your own identity.” The prelate’s face is that of a smiling faith entering the conclave to elect for the first time the successor of Peter despite extreme poverty and a thousand wounds Subscribe to Asia News updates or change your preferences In the fast-paced world of the English Premier League opportunities can come and go in the blink of an eye For Angolan center-back David Mota Veiga Teixeira do Carmo a season-long loan spell at Greek giants Olympiacos is proving to be the perfect platform to showcase his immense talent and stake a claim for a return to his parent club who joined Forest in 2024 after an impressive stint in Portugal’s Primeira Liga with Braga and more recently Porto found regular game time elusive in England Seeking consistent minutes to hone his skills Nottingham Forest loaned Carmo to Olympiacos at the start of the 2023/24 season The towering defender has since gone from strength to strength Since arriving in Piraeus in what is his second spell Carmo has cemented himself as a pivotal figure in Olympiacos’ backline Standing tall at 6’5″ with a powerful frame and an innate sense of positioning the Angolan has quickly adapted to the demands of the Greek Super League and knack for well-timed challenges have made him a standout performer in a league known for its physicality The stats also suggest that Carmo has been almost indispensable in Olympiacos’ defense He’s also made an average of 0.75 tackles and 1.49 interceptions per 90 minutes Carmo has also racked up an average of 3.28 clearances and 3.87 aerial duels per 90 within the same time period and 96th percentiles amongst players in the Greek Super League He has been instrumental in the team’s strong defensive record his leadership qualities have shone through with teammates and coaching staff alike praising his vocal presence and ability to organize the defense Carmo’s success at Olympiacos is both a testament to his potential and a tantalizing prospect for the club’s future With Forest striving to solidify their defensive options in the Premier League a return for the Angolan could provide the club with a ready-made solution rather than having to dip into the summer transfer market Beni Mukendi: Casa Pia’s Angolan Gem Carmo will bring important qualities that will bolster the Forest backline—athleticism and versatility to play in either a back three or four including a UEFA Europa Conference League crown with Olympiacos last season something that could prove invaluable in England’s top flight While Nottingham Forest may view Carmo as an asset to recall possibly with a view to pairing him with the largely impressive Murillo Olympiacos are reportedly keen to extend his stay either through a loan extension or a permanent deal The Greek side values his contributions and believes he can be a long-term defensive stalwart Nottingham Forest face a decision: recall Carmo to bolster their squad or allow him to continue thriving in Greece one thing is clear—David Carmo has positioned himself as a player to watch Angolan fans and Olympiacos supporters can revel in his performances knowing they are witnessing a star on the rise Whether in the red of Forest or the stripes of Olympiacos Featured Image: @GabFoligno / Quality Sport Images – Getty Images Sign in Join now, it's FREE! It is with great sorrow we announce the passing of Maria Do Carmo Albuquerque on March 31/2025 in her 89th year at home beside her loving children Maria Do Carmo was predeceased by her husband of 61 years Maria Do Carmo was loved by and will be sadly missed by her children Carminda (Carlos) Mike (Jenny) Albuquerque and greatly missed by Julie Leal-Albuquerque Maria will also be missed by her grandchildren Randy Maria Do Carmo was born in Santa Maria Acores one of eleven children to predeceased Antonio Freitas and Maria Jose Moura Maria Do Carmo is survived by sisters Guiomar Resendes Maria Do Carmo was predeceased by beloved sisters Francelina Resendes and brothers Jose Freitas brother-in-law Luis and Rafael Albuquerque and sister-in-law Maria Costa N Cambridge where Family and Friends will be received on Wednesday April 2 Mass of Christian burial will be held from Our lady of Fatima R.C More Spotlight > It is with great love and sadness that we announce the loss of Amandio Costa where he learned the value of hard work on his family farms with their infant son immigrated to Canada in 1964 they worked tirelessly to establish their orchard business and create a life rooted in love and in family gatherings filling his home with music and laughter Antonio (Tomoko) and Lidia (Todd); and his cherished granddaughters Breanna (Jordy) He now goes to join family members who passed before him Amandio’s strong presence and determination was always inspiring and comforting for his friends and family but we find comfort in knowing he lived a full life filled with love and is now resting in peace with his beautiful wife Father — thank you for sharing your extraordinary life with us please join the family online by Livestream at the time of the service (see below) a recording will be made available to view in 3–5 days Amandio will be laid to rest on the same day at 1:00 PM at Kelowna Memorial Park Cemetery: 1991 Bernard Ave If you wish to attend the Funeral Mass, please Click Here to Register please scroll down the page to the area called “Condolences” at 4:30 PM at Springfield Funeral Home at located at 2020 Springfield Road Amandio do Carmo is scheduled for Funeral Mass: MONDAY Below you will find the map for the service location and the contact information should you have any questions » Condolences sent through this page can be seen by the public. If you wish for your condolence to go to the family privately, please send it to: " + EmailId +" » Condolences sent through this page can be seen by the public. If you wish for your condolence to go to the family privately, please send it to: contact@springfieldfuneralhome.com Amândio will be remember as long as I live for his jovial attitude May he find rest after all the hard work in this life My heart goes out to you all on the loss of Amandio and I am grateful for the opportunity he gave me early in my career as an architect to design your parent’s new home You are in our thoughts and prayers at this time Dear Tony and family – My deepest sympathies to you all in this season of loss Keeping you much in my thoughts and prayers at this time Although I never had the pleasure of meeting Amandio in person I heard only wonderful stories about the man the husband the brother the father and grandfather » To share your condolence on this page please fill out the requested fields click the 'Choose File' button and select a photo from your computer » Your email is never published or shared NOTE: all messages will go through an administrative process before being posted Δdocument.getElementById( "ak_js_1" ).setAttribute( "value" Everything was so organized and pertinent to what we needed courteous and very helpful during our emotional and challenging time They were so prompt on answering any questions and changing things They were prepared and respected any religious decisions The process was so seamless and made easy in such a high stress situation In the past 2 years Springfield has helped us through the death of my Mother and my Father Their response and help allowed us to focus on the remaining family rather than worrying about details of the funeral and all the notifications that are required upon a death The atmosphere has been nothing but professional during times of grief the staff coached me on the next steps and looked after filling in all the government forms that I needed to sign which took a huge burden off my mind at the time From the time I called to the completion of all the services There was a sense of caring from each staff member I was turning over my Mom to their care and I felt very comfortable with everyone I felt heard and never felt pushed into any decision I have always found the team at Springfield Funeral Home to be VERY caring I have and will continue to recommend them to anyone who asks which funeral home would I suggest they use No other funeral home I have dealt with even comes close to Springfield Funeral Home I was made to feel as if I was the only one they had to serve Everything that was arranged for us was perfect Thank you for making this difficult time a little more acceptable via your staff’s obvious caring and respect I liked the personal treatment given to my mother who is 97 years old I found Springfield employees pleasant and sincere was that the funeral home would help me get through the paperwork need at this time Since this was my first experience (with a funeral home) everything was above and beyond what I expected Thank you to your team for your kindness to me at a very challenging time You have now taken care of both of my parents with professionalism and care Springfield Funeral Home is always professional We appreciate that you have dedicated staff for all needs from planning the service to completing government paperwork I am not sure there was anything you could have done to make a very intense emotional time less stressful Although we hadn’t expected Ken to want a service when he said we needed to have one for us not him Your sincerity and compassion meant everything to us your compassion and professionalism is truly amazing super professional and caring as each guest arrived Keep up the good work that you do as it is such an important service you provide It is still the most difficult time in a person’s life We appreciated the peace of mind that everything was being looked after You provide a wonderful service for people going through a traumatic time The kindness and professionalism shown by the staff at Springfield Funeral Home was exemplary READ MONOGRAPH Paxlegal has announced the strengthening of its legal team with the integration of Murillo Costa Sanches and Mariana Andrés (all pictured from left to right) led by António Patrício and Bárbara Pestana Murillo Costa Sanches has experience in civil and commercial Law with a particular focus on real estate and corporate law where he was head of the real estate department he joins as associate and will focus his practice on real estate and corporate He holds a law degree from the Faculty of Law of the University of Coimbra and is pursuing a master’s degree in civil law at the same institution Maria do Carmo Pereira Coutinho joins as an associate Her practice will focus on labour law and business development She holds a law degree from the Faculty of Law of the University of Lisbon Mariana Andrés holds a law degree from the Faculty of Law of the University of Lisbon providing support to the team of lawyers across various practice areas founding partner of Paxlegal: “In recent months we have witnessed significant growth in our activity which has led us to reinforce the team in strategic areas We are confident that their integration will be an asset to the firm and A&G Global Investors acquires renewable energy land portfolio… It represents the main source of information in the legal business sector in Spain and Portugal The digital magazine – and its portal – address to the protagonists of law firms and in-house lawyers The magazine is available for free on the website and on Google Play and App Store information about deals and their advisors For further information, please visit the Group’s website www.lcpublishinggroup.com It was imperative the Reds sorted out the rearguard with last season proving a shambolic mess at times defensively Nikola Milenkovic was the star central defensive signing, with Alex Moreno coming in to bring balance to the full-back areas One player who proved a bit of a surprise signing was Portuguese defender David Carmo with the defender immediately heading to Olympiacos on loan The player helped the Greek outfit win the Europa Conference League last season and Evangelos Marinakis did a deal that might benefit Forest in the long run we’ve not seen much of Carmo so we enlisted the help of Olympiacos supporter we asked him how he’d started at Olympiacos on the back of last season’s hugely successful campaign Stam exclusively told Nottingham Forest News: “Olympiacos made a great effort to keep him this year and finally succeeded with the help of Nottingham Forest The player missed the entire pre-season and only on August 31st he played for the first time in the red and white again “If we take stock of the first two months of Carmo back at Olympiacos I think we have to agree that we expected better things from the centre-back but he’s certainly not been as good as he was at the end of last season He needs to figure out how to become a safe bet for the team’s defence again.” Although his second spell with Olympiacos hasn’t quite started with red-hot form there are clear strengths when it comes to his defensive game Stam continued: “He is a 6ft 5in left-footer has a good long pass on him and is dominant in the aerial challenge.” we asked Stam if there was a Forest player who he felt Carmo could be deemed similar to and he delivered an intriguing response He told us: “I’m no expert on Forest but he reminds me of Nikola Milenkovic We face him in the European final against Fiorentina I see Carmo as a big-league player and if he rediscovers last season’s form he can play for Nottingham Forest Stoppers of his size who aren’t slow are not easy to find.” this deal already feels like a complete no-brainer Forest parted with just £10 million to sign the player from Porto with Olympiacos likely to have paid a fee to take him on loan The Reds might lose Murillo next summer and with Carmo being left-sided the club might already have signed his replacement These comments certainly sound positive from a Forest perspective The hope will be that Carmo can stay in the team this season and get himself back to the form he showcased last season when Olympiacos claimed a European title he should arrive at Forest bang in form and brimming with confidence Nikkei serves Peruvian-style sashimi (left) and Creole ceviche The location of chef Wataru Saeki’s new restaurant the indoor and outdoor music venue next to The Broad movie theater Nikkei puts the chef within listening distance of his beloved New Orleans music given that live New Orleans music is the reason he moved here from Japan 600 N. Broad St., (504) 224-5657; nikkeiizakaya.com A Japanese-style tavern from the team behind Carmo “I loved The Meters and New Orleans music so much also techno and house music from Detroit,” says Saeki Although he went to school to be a computer engineer He worked at a noodle shop after college to save enough money to move here I don’t have the mentality to make that move He got a job working for Hisako "Momo" Young at Ninja on Oak Street where he learned how to make sushi and practiced his craft The Honns opened Carmo in the Warehouse District 15 years ago Seiki wanted to experience something besides Japanese cuisine and then together the trio launched Nikkei in late November The Japanese tavern pays homage to “nikkei,” the term for Japanese immigrant communities and explores Japanese cuisine using a bounty of Louisiana ingredients This new project is an exciting one for all three partners “I’d worked with (Broadside) owner Brian (Knighten) before,” Dana Honn says When I had the first meeting about this space There’s a turntable for vinyl and a stack of albums that includes the Rebirth Brass Band A photo of Fats Domino offers a sense of place along with art including Japanese-style wood cuts and a handsome pelican portrait local draft beer and imported Japanese bottles House cocktails include a hibiscus margarita and a seasonal old-fashioned Fans of Carmo will recognize the chef’s deft hand at work in the Nama/Raw section of the menu The Gulf tuna tartare and Peruvian tiradito were in regular rotation at Carmo The Honns bring in pristine and sustainably sourced fish And the chef is proud to offer “the very best rice.” The frequently changing menu includes broiled fish collars with ponzu and aioli There’s a version of Peruvian nikkei causa which are chilled whipped potatoes topped with avocado “That’s my daughter Mona’s favorite,” says the chef Natasha and Leonard Arthur told us about learning to make fry bread starting their pop-up and serving the food they grew up on Popular pork and chicken katsu sandwiches feature panko-breaded fried cutlets dressed with cabbage slaw on a brioche bun and are served with pabu (pub) hand-cut fries a deep-fried fusion of rice paper and seaweed The outstanding Nikkei broil is a plate of Gulf oysters Everything except for Monday’s ramen special is gluten free with wheat-free tamari used instead of regular soy sauce and there’s an all-day Tuesday $6 special on drinks It lines up with the Broad Theater’s Tuesday special of $6 tickets He is working closely with Broadside general manager and beverage maven Lara Desmond to put together a calendar who moved nearly 7,000 miles from home to hear New Orleans music It’s big news that New Orleans chef Melissa Araujo opened a second restaurant in a much bigger location in Mid-City Email Gambit's food and drink team at dining@gambitweekly.com Email notifications are only sent once a day, and only if there are new matching items. Your browser is out of date and potentially vulnerable to security risks.We recommend switching to one of the following browsers: Volume 8 - 2014 | https://doi.org/10.3389/fnbeh.2014.00321 Intraneuronal accumulation of amyloid β (iAβ) has been linked to mild cognitive impairment that may precede Alzheimer's disease (AD) onset This neuropathological trait was recently mimicked in a novel animal model of AD the hemizygous transgenic McGill-R-Thy1-APP (Tg+/−) rat The characterization of the behavioral phenotypes in this animal model could provide a baseline of efficacy for earlier therapeutic interventions The aim of the present study was to undertake a longitudinal study of Aβ accumulation and a comprehensive behavioral evaluation of this transgenic rat model spatial learning and reference memory at 3 Western blots and semiquantitative immunohistochemistry in hippocampal samples SDS-soluble Aβ peptide accumulated at low levels (~9 pg/mg) without differences among ages Western blots showed SDS-resistant Aβ oligomers (~30 kDa) at 6 and 12 months male Tg+/− rats exhibited a spatial reference memory deficit in the Morris Water Maze (MWM) as early as 3 months of age Tg+/− rats displayed a working memory impairment in the Y-maze and higher anxiety levels in the Open Field (OF) at 6 and 12 months of age Exploratory activity in the OF was similar to that of WT at all-time points Spatial learning in the MWM and the recognition memory as assessed by the Novel Object Recognition Test The data from the present study demonstrate that the hemizygous transgenic McGill-R-Thy1-APP rat has a wide array of behavioral and cognitive impairments from young adulthood to middle-age The low Aβ burden and early emotional and cognitive deficits in this transgenic rat model supports its potential use for drug discovery purposes in early AD This discrepancy can be explained by differences in the genetic background and the transgene expressed working memory and the temporal progression of AD-like non-cognitive behavioral abnormalities which are affected early during the course of AD were not determined in this novel transgenic animal model Multiple behavioral tasks should ideally be used to characterize the phenotypes of potential animal models of AD since in the clinical setting, a wide range of neuropsychological tests are employed to determine the profile of the cognitive impairment (Gainotti et al., 2014; Webster et al., 2014) by using a longitudinal assessment procedure we sought to determine the levels and accumulation of soluble Aβ and iAβ peptides and the development of impairments in the exploratory activity emotional behavior and working and spatial reference memory in Tg+/− rats we will test if this model recapitulates some of the biochemical emotional and cognitive alterations seen in pre-symptomatic AD Our results could be relevant to define in this animal model the critical window for therapeutic interventions that may be applied to early stages of AD The study was carried out in strict accordance with the guidelines of the OLAW-NIH (Office Laboratory Animal Welfare) The protocol was approved by the local Animal Care Committee of Fundación Instituto Leloir (FIL) Assurance# A5168-01 Hemizygous transgenic McGill-R-Thy1-APP (Tg+/−) rats, harboring the human APP751 transgene with the Swedish and Indiana mutation under the control of the murine Thy1.2 promoter (Leon et al., 2010) were provided to Fundación Instituto Leloir (FIL) by The Royal Institution for the Advancement of Learning/McGill University An in-house breeding colony was established at FIL by cross-breeding Tg+/− males with wild-type Wistar females rats Each litter was culled to 10 pups (up to 8 males per litter were left) dams were removed from the litters and pups were genotyped as described below When the results of the genotyping were available (between 22 and 23 post-natal days) rats were pseudorandomly assigned to experimental groups groups were made up of pups from 3 to 4 different litters Animals were maintained on a 12-h light/12-h dark cycle group-housed 2–4 per cage and had ad libitum access to water and a standard rodent diet Tissue samples were obtained by ear punches from the litters to determine the genotype of each rat Genotyping was performed using a validated protocol genomic DNA was prepared from ear biopsy tissue using proteinase K digestion followed by phenol/chloroform/isoamyl alcohol extraction (Life Technologies) DNA quantification was determined in a Nanodrop 2000 spectrofotometer Samples with a ratio of absorbance at 260/280 and 260/230 nm above 1.80 were used for PCR to recognize whether or not the rat carried the human APP (hAPP) gene The following sets of primers were used: APP-F 5′-AGGACTGACCACTCGACCAG-3′; APP-R 5′-CGGGGGTCTAGTTCTGGAT-3′; CyclophilinG-F 5′- TACAACAGTAGAACAAGGGAGCGAAG-3′ and CyclophilinG-R 5′-ATCCCTCCTTCTTCTCCTCCTATCTTT-3′ Cycling conditions were: 95°C (15 min) and 40 cycles of 94°C (30 s) 72°C (1 min) followed by one step at 72°C (2 min) and kept at 4°C until processed PCR products were resolved by 2% agarose mini-gel electrophoresis in TBE buffer and visualized with ethidium bromide (10 mg/ml) staining under UV light Cyclophilin G amplicon (248 bp) was used as a housekeeping gene to verify DNA integrity and PCR conditions The hAPP amplicon (387 bp) indicates the presence of the human transgene To verify the hemizygocity of the transgenic rats quantitative PCR (qPCR) was performed by using the same set of hAPP primers as described above and the following set of GAPDH (used as housekeeping gene) primers: GAPDH-F: 5′-GGGGAAGGACGCTGTACGGG-3′ and GAPDH-R: 5′-AAGGGGAGC AACAGCTGGGGT-3′ A total of 1 ng of genomic DNA was used per well (5 μl of a 0.2 ng/μl dilution) SYBR-Green qPCR was performed by using the KAPA SYBR® FAST Universal 2X qPCR Master Mix (Kapa Biosystems) Reactions were run in a Stratagene Mx3005P cycler and results analyzed by the MxPro software in a Comparative Quantitation mode In all of the runs a genomic DNA sample of a hemizygous animal was included and used as an internal calibrator the relative quantity (dRn) value should be between 0.8 and 1.3; if the unknown sample is homozygous the result is close to a value of 2 (between 1.8 and 2.3) Only male rats were used to avoid any potential effects of changes in the female estrus cycle on behavioral performance Three groups (n = 3/group) of naïve Tg+/− male rats of 3, 6, and 12 months of age, and one group (n = 3) of naïve WT male rats of 12 months of age, were anesthetized with ketamine (50 mg/kg) and xylacine (10 mg/kg), perfused transaortically with 0.9% sodium chloride with 200 U/l heparine and brains were quickly removed as described previously (Leal et al., 2006) Brains were divided into left and right hemispheres Hippocampus was isolated from left hemisphere and retained for biochemical studies The right hemisphere was post-fixed in 4% paraformaldehyde in 0.1 M phosphate buffer (PB) and finally transferred to a solution of 30% sucrose in 0.1 M PB for 5 days or until sectioned for immunohystochemistry into 40 μm coronal sections with a freezing sledge microtome (SM 2000R None of the animals assigned to immunohistochemical and biochemical experiments were submitted to behavioral testing Immunoreactivity was detected by Western blot using an enhanced chemiluminescence detection system (ECL Thermo Scientific) and membranes analyzed and quantitated with a STORM 840 Phosphor Imager (GE Healthcare) Group values were obtained simultaneously and normalized with respect to APP immunoreactivity Stereological estimation of the hippocampal volume affected by iAβ is described below in Image analysis Section these tests were conducted between 8:00 a.m testing order of the groups was counterbalanced to avoid the confounding effect of time of the day at which animals were tested The order of tests were as follows: Elevated Plus Maze (EPM) test Y-maze spontaneous alternation (Y-Maze) test The interval between tests was at least 24 h the entire apparatus and objects in the NORT were cleaned with 70% ethanol to prevent a bias due to olfactory cues All testing sessions were digitally recorded (JVC Everio GZ-HD620) and analyzed using a computerized video-tracking system (Ethovision XT The Netherlands) or the ethological observation software JWatcher V1.0 All the experimenters were blinded to the genotype of the animals The EPM test assesses anxiety-like behaviors (Pellow et al., 1985) The apparatus consisted of a black melamine central square platform (11 × 11 cm) from which four black melamine arms radiate (50 × 11 cm) separated from each other by 90° Two of the arms (“closed arms”) have a wall (40 cm in height) all around its perimeter but not in the entrance and the other two arms (“open arms”) do not have any wall but with raised edges (0.25 cm) around its perimeter The maze was elevated one meter from the floor by five legs Light intensity in the open arms was 80–90 lux Each rat was placed onto the central platform facing an open arm and allowed to freely explore the maze for 5 min An arm entry was counted when rat introduced its four paws into an arm The following dependent variables were recorded: total distance moved percentage of open arm entries (N° open arm entriesTotal entries × 100) and percentage of time spent in open arms (Time spent in open arms300s × 100) The OF test was used to assess spontaneous exploratory activity and anxiety-related behaviors (Walsh and Cummins, 1976) The apparatus consisted of a black melamine square (60 × 60 cm) surrounded by walls of 40 cm in height A central area was arbitrarily defined as a square of 30 × 30 cm and it was drawn over the image of the OF in the video-tracking system A rat was considered to be into the central area when its four paws were on it Light intensity in the center of the OF was 70–80 lux Each rat was placed in the center of the maze and its behavior was analyzed for 5 min number of entries into the central area and time spent in central area The NORT evaluates recognition memory of one previously explored object (familiar) compared with one novel object (non-familiar) (Ennaceur and Delacour, 1988; Ennaceur, 2010) NORT was performed in the same OF apparatus 24 h after the OF test Rats were tested during two trials (sample and retention trials) separated by an interval of 1 h during which they were returned to their cages each rat was faced with two identical objects (called familiar objects) placed in a symmetrical position and the time exploring freely each object was recorded one of the two familiar objects was replaced by a novel object and the time exploring each was recorded Sets composed of three copies of the same object were used to prevent odor cues and all combinations and location of objects were used to prevent bias due to preference for a particular object or location Exploration time was computed when the snout pointed to the object at a distance ≤2 cm Discrimination index (d1) and discrimination ratio (d2) scores were calculated using the following formulas: d1 = tn − tf and d2=tn−tftn+tf where tn = the amount of time rats explored the novel object and tf = the amount of time rats explored the familiar object An arm entry was scored when rat placed the four paws within that arm The following dependent variables were registered: total number of arm entries number of triads (sequence of three consecutive visits to different arms) An alternation was defined as an entry into three different arms on consecutive choices The percentage of alternation was calculated as the ratio of actual to maximum number of alternations The maximum number of possible alternations was defined as the total number of arm entries minus 2 A low percentage of alternation is indicative of an impaired spatial working memory because the rat cannot remember which arm it has just visited and thus shows decreased spontaneous alternation The apparatus used was previously described in detail by Galeano et al. (2011) it consisted of a circular galvanized steel pool (180 cm in diameter and 60 cm in height) filled with water at 22 ± 1°C to a depth of 36–40 cm and imaginarily divided into four quadrants: North (N) A cylindrical platform (10 cm in diameter and made of transparent acrylic plastic) was placed 2 cm above (visible escape platform) or beneath the water surface (hidden escape platform) in the center of one of the quadrants To enhance the visibility of the platform during the cued learning training a “flag” was attached to the platform The pool was located in the center of an experimental room with multiple extra-maze visual geometric cues hanging on the wall Indirect illumination was provided by four spiral compact fluorescent lamp in each corner facing the walls The following dependent variables were recorded: latency to the platform the number of crossings over an annulus zone of 20 cm in diameter (defined as a zone surrounding the location where the platform was located during the spatial learning training) and time spent in the outer ring of the pool (25 cm wide) During cued learning the platform protruded 2 cm above water surface and a “flag” was attached to it (visible escape platform) The maze was surrounded by black curtains to minimize the availability of extra-maze cues For each of the four trials conducted on each day the platform was moved to a different quadrant and a different start location was used If a rat had not located the platform before 120 s elapsed it was gently guided to the platform location and was allowed to remain there for 15 s Inter-trial interval duration was 20–30 min Spatial learning and reference memory. We used procedures described previously with some modifications (Vorhees and Williams, 2006; Galeano et al., 2011) Black curtains were removed and the spatial learning phase of the task was performed over five consecutive days with four trials per day rats were released into the pool from one of the four starting positions (one per each quadrant) and the order of the sequence was changed pseudorandomly between days Animals were able to escape from the water using the hidden escape platform that was kept in the same location throughout the five spatial learning sessions A trial was finished when the animal found the escape platform or when 120 s had elapsed Rats remained on the platform for 15 s with an inter-trial interval of 20–30 min Twenty four hour after the last trial of the learning phase spatial reference memory was assessed with a probe trial in which the escape platform was removed from the pool and rats were released from a new starting position not used during the learning phase the number of annulus crossings and the time spent in the outer ring of the pool (thigmotactic swimming) was recorded After each trial rats were dried and when the session finished they were returned to their colony room For stereological estimations the contours of ventral and dorsal hippocampus showing immunoreactivity for iAβ was drawn in each section with Image J software (NIH, USA) tool to determine the affected area. The Cavalieri's principle (Gundersen and Jensen, 1987) was used to calculate the affected volume by using the following formula: V = ∑AxNxE where V = volume (mm3); ∑A = sum of area counted (mm2); N = number of sections analyzed; E = thickness of each section (mm) All data are shown as the mean ± s.e.m Data were analyzed by Two-Way ANOVA or by Two-Way mixed ANOVA tests followed by Tukey's HSD post-hoc tests for multiple comparisons USA) was used to perform all statistical analyses Our data strongly suggest a subtle phenotype in Tg+/− rats with an “age-independent” accumulation for SDS-soluble monomeric Aβ40 and iAβ but an “age-dependent” accumulation for SDS-resistant Aβ oligomers SDS-resistant Aβ oligomers and intracellular Aβ (iAβ) accumulation in Tg+/− rats (A) Representative Western blotting showing APP expression and Aβ oligomers by using 6E10 monoclonal antibody Hippocampal samples of 6 and 12-month-old Tg+/− rats contained Aβ oligomers (~30 kDa) in contrast to 3-month-old Tg+/− or 12-month-old wild-type (WT) littermates (B) iAβ is present in the hippocampus and cortex (Cx) of Tg+/− animals at 3 magnification of CA1 region decorated with iAβ (arrows) in the neurons of granular layer Hippocampal volume affected with iAβ is similar among different ages analyzed (lower panel) Three groups (n = 3/group) of naïve Tg+/− male rats of 3 and one group (n = 3) of naïve WT male rats of 12 months of age were assessed These animals were not submitted to behavioral testing cohorts' characteristics and behavioral tests employed and 12 months old hemizygous transgenic McGill-R-Thy1-APP (Tg+/−) rats and their wild-type (WT) littermates were assessed in a behavioral test battery including: Elevated Plus Maze (EPM) test spontaneous alternation in the Y-maze test (Y-maze) and cued and spatial reference memory versions of the Morris Water Maze (MWM) test a neurological screening (N.S.) was performed and animals were euthanized after completion of behavioral testing Gross inspection of each rat prior to the behavioral experiments did not reveal any visible differences between WT and Tg+/− animals All animals appeared healthy and neurological reflexes were all scored as being present indicating that levels of anxiety were similar among all experimental groups Locomotor activity and anxiety-related behaviors of hemizygous transgenic McGill-R-Thy1-APP (Tg+/−) rats and wild-type (WT) littermates in the Elevated Plus Maze (EPM) test No differences in anxiety-related behaviors were observed between Tg+/− and WT rats at any age (C,D) The number of animals tested was as follows: 9 WT and 10 Tg+/− at 3 months and 11 WT and 11 Tg+/− at 12 months exploration and anxiety-related behaviors of hemizygous transgenic McGill-R-Thy1-APP (Tg+/−) rats and wild-type (WT) littermates in the Open Field (OF) test 6 and 12 months old rats showed less locomotion and rearing activity than 3 months old rats (A,B) Tg+/− rats of 6 and 12 months of age showed increased anxiety with a lower number of entries into center (C) and less time spent in the central area (D) than WT littermates These results demonstrate that WT and Tg+/− rats were able to recognize the novel object from the familiar one at all ages tested Performance of hemizygous transgenic McGill-R-Thy1-APP (Tg+/−) rats and wild-type (WT) littermates in the Novel Object Recognition Test (NORT) both genotypes recognized the novel object The dashed lines indicate an equivalent exploration time for the two objects (chance level) *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 vs These results indicate that Tg+/− rats displayed a spontaneous alternation impairment at 6 and 12 months of age Spontaneous alternation in the Y-maze test 12 months old rats showed less total number of arm entries than 3 and 6 months old rats (A) hemizygous transgenic McGill-R-Thy1-APP (Tg+/−) rats exhibited an impairment in spontaneous alternation compared with wild-type (WT) groups (B) At the three ages studied, post-hoc multiple comparisons showed that, during the second day, both genotypes significantly reduced their latency to reach the visible platform to similar levels (p < 0.01 for all cases; see Figures 7A–C) [Two-Way mixed ANOVA test 17) < 1; Day × genotype: F(1 20) < 1; Day × genotype: F(1 These results suggest that transgenic groups were not visually impaired and showed no deficit in swimming ability Hemizygous transgenic McGill-R-Thy1-APP (Tg+/−) rats showed an unimpaired cued learning in the Morris Water Maze (MWM) test Escape latency of wild-type (WT) and Tg+/− groups at 3 (A) both genotypes reduced their escape latencies to similar levels from day 1 to day 2 Values are shown as the mean ± s.e.m **p ≤ 0.01; ***p ≤ 0.001 vs both genotypes displayed similar swimming speeds at all ages (see Figure 1 in Supplementary Material) suggesting similar levels of motivation to solve the task Spatial learning of hemizygous transgenic McGill-R-Thy1-APP (Tg+/−) rats and wild-type (WT) littermates in the Morris Water Maze (MWM) test Escape latency and path length of Tg+/− and WT rats at 3 (A,B) both genotypes reduced their escape latencies and paths length at an equivalent rate across days these results indicate that Tg+/− rats had a significant impairment to remember the location of the platform as compared to WT animals Regarding swimming speed and thigmotactic swimming no differences were detected in probe trials (see Figures 1 Spatial reference memory impairment in hemizygous transgenic McGill-R-Thy1-APP (Tg+/−) Percentage of time spent in each quadrant of the Morris water maze and number of annulus crossings during the probe trial at 3 (A,B) WT groups spent significantly more time in the quadrant where the platform was previously located (target quadrant) (A,C,D) Tg+/− groups spent similar amount of time in each one of the four quadrants (A,C,D) Tg+/− groups crossed the annulus zone significantly less times than WT groups (except at 6 months of age when a strong tendency was detected; B,D,F) indicating a spatial reference memory impairment Dashed lines indicate the percentage of time spent in each one of the quadrant predicted by chance (25%) percentage of time spent in target quadrant; *p ≤ 0.05 As it was mentioned in the “Materials and Methods” Section the groups of animals that underwent the behavioral assessment were not the same used to study Aβ accumulation This effect could be attributable to the levels of illumination used (80–90 lux in the open arms) To rule out the possibility of a floor effect that could be masking an anxiety phenotype in Tg+/− rats in the EPM future studies are needed to investigate the behavior of these animals in this test under different experimental conditions In summary, even if the emergency (Holmes, 2001) or the light/dark box (Pinton et al., 2011) tests were not assessed in this study our report is the first analysis of the anxiety disturbances in a transgenic rat model of early AD that does not develop Aβ plaques It is of interest that the anxiety pattern described here for the Tg+/− rats appear to closely mimic neuropsychiatric symptoms described in some pre-clinical AD patients we did not find in Tg+/− rats any impairment in the learning phase of the MWM test These discrepancies can be explained both by the number of animals used and by the gender of the animals utilized (males and females in the first report while males only in our study) Although both tests are hippocampal-dependent the NORL is a more complex task that includes the spatial component that is absent in the classic NORT test and may explain the discrepancy between results While in the former test one unfamiliar object had to be discriminated among a total of five objects during the novel object recognition phase of the task we employed a more classical approach with two dissimilar objects (one unfamiliar and one familiar) that had to be discriminated during the retention trial Discrepancies in results reveal that when the complexity of the task is increased recognition memory may be also impaired in this transgenic rat model These observations are in agreements with our results since Tg+/− rats lack parenchymal Aβ plaques but thus accumulate soluble oligomers intraneuronally we investigated emotional and cognitive symptoms in Tg+/− rats The most relevant result is that transgenic rats showed from adulthood (6-month-old) to middle age (12-month-old) increased anxiety levels and working memory impairment transgenic rats showed an impaired spatial reference memory in comparison to WT rats This deficit was also observed at 6 and 12 months of age recognition memory and spatial learning were spared Given that Tg+/− rats capture many behavioral features of early stages of AD this new model may represent an important tool for the neuroscience community to enable future studies in basic and translational AD research The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest This work was supported by grants from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET-PIP 693) and Fundación Florencio Fiorini (to Laura Morelli) Alzheimer's Association (IIRG-11-205127) (to Eduardo M Castaño) and Canadian Institute for Health and Research (MOP-97776) (to A Martino Adami are supported by CONICET fellowships Francisco Capani and Laura Morelli are investigators of CONICET Claudio Cuello is holder of a McGill University Charles E We would like to thank Tamara Logica Tornatore Mariana Inés Holubiec and Juan Ignacio Romero for their helpful assistance during the behavioral tests The Supplementary Material for this article can be found online at: http://www.frontiersin.org/journal/10.3389/fnbeh.2014.00321/abstract Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Using pictures and words to understand recognition memory deterioration in amnestic mild cognitive impairment and Alzheimer's disease: a 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defining the preclinical stages of Alzheimer's disease: recommendations from the National Institute on Aging-Alzheimer's Association workgroups on diagnostic guidelines for Alzheimer's disease Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Differences in fear motivated behaviors among inbred mouse strains Pubmed Abstract | Pubmed Full Text | CrossRef Full Text doi: 10.1207/S15327663JCP1001&2_03 CrossRef Full Text Designing drug trials for Alzheimer's disease: what we have learned from the release of the phase III antibody trials: a report from the EU/US/CTAD Task Force Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Circadian phase and sex effects on depressive/anxiety-like behaviors and HPA axis responses to acute stress Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Morris water maze: procedures for assessing spatial and related forms of learning and memory Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Using mice to model Alzheimer's dementia: an overview of the clinical disease and the preclinical behavioral changes in 10 mouse models Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Accelerated nervous system development contributes to behavioral efficiency in the laboratory mouse: a behavioral review and theoretical proposal Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Working memory deficits in retinoid X receptor gamma-deficient mice Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Cuello AC and Morelli L (2014) Longitudinal analysis of the behavioral phenotype in a novel transgenic rat model of early stages of Alzheimer's disease Received: 11 July 2014; Accepted: 29 August 2014; Published online: 16 September 2014 Copyright © 2014 Galeano, Martino Adami, Do Carmo, Blanco, Rotondaro, Capani, Castaño, Cuello and Morelli. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) distribution or reproduction in other forums is permitted provided the original author(s) or licensor are credited and that the original publication in this journal is cited in accordance with accepted academic practice distribution or reproduction is permitted which does not comply with these terms *Correspondence: Laura Morelli, Laboratorio de Amiloidosis y Neurodegeneración, Fundación Instituto Leloir, IIBBA (CONICET), Av. Patricias Argentinas 435, C1405BWE, Ciudad Autónoma de Buenos Aires, Argentina e-mail:bG1vcmVsbGlAbGVsb2lyLm9yZy5hcg== Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher 94% of researchers rate our articles as excellent or goodLearn more about the work of our research integrity team to safeguard the quality of each article we publish The Catholic faithful of Timor-Leste have been waiting for Pope Francis’ visit for a long time Joy permeates the streets of this small nation and then occupied by the Indonesian military until 1999 When the Pope’s visit to Timor-Leste was announced the entire population welcomed the news with enthusiasm joy and a desire to welcome the Holy Father Pope Francis will be the second Pontiff to visit the country who travelled to Timor-Leste on 12 October 1989 Pope Francis will be the first Pope to visit Timor-Leste as a new sovereign nation that gained its independence on 20 May 2002 The visit to Timor-Leste is part of the papal trip in Asia and the Pacific the government of Timor-Leste and the local Church set up a joint team The two institutions have been working well together from the start The solemn Eucharistic celebration that will take place on 10 September will be held in a strategic location which will gather Timorese faithful and a number of pilgrims from Indonesia and Australia Some 700,000 people are expected to participate the Pope will also meet with young people at the “Centro de Convenções”; more than 4,000 young people will likely attend seminarians and catechists in the cathedral of the Immaculate Conception He will then meet with children with disabilities from the “Irmãs Alma” school The Pope’s visit to Timor-Leste will be an important event for building the faith and affirming the cultural and religious identity of the Timorese people The central theme of the visit is: May your faith be your culture. Faith was one of the important pillars in the fight for freedom which allowed us not to lose hope in the long moments of suffering The liturgical commission has circulated a short prayer for parishes religious communities and religious formation houses to say after daily and Sunday Mass The intentions of this prayer are for Pope Francis’ visit and health the objective of the Timorese Bishops’ Conference is to help the Christian faith live and grow with more ardour in the heart of the Timorese people The bishops have set up some meetings to inform and form the faithful from the three Dioceses of Timor-Leste: Dili The theme of formation and catechesis is tied to the Pope’s primacy and to Pope Francis as a person The goal is precisely to help the faithful deepen their understanding of the primacy of the Pope and of Pope Francis’ teachings but also to urge them to harmonize faith and local culture International speakers from Indonesia and the Philippines intervened as did national speakers who presented their own ideas The theme of the first academic seminar was: Asian culture and the Christian faith. There were three speakers: Verbite Fr professor at the Catholic University of Timor-Leste; and a Franciscan missionary and professor at the “Dom Jaime Garcia Goulart” Institute of Philosophy and Theology in Dili The theme of the second academic seminar was: The Christian faith in a multicultural context professor at the national university of Timor-Leste a national retreat will be led by Fr Antonius Eddy Kristiyanto The Church in Timor-Leste hopes that Pope Francis’ presence will bring peace and hope to the Christian faithful and transform them interiorly The local Church also hopes that with the Pontiff’s presence among us the Catholic faithful will understand their faith celebrate it and bear witness to it in their daily lives The Christian faith should become culture in the heart of the country’s Catholic Christians as Pope Francis said in his address to Timorese bishops on the occasion of their ad limina  visit at the Vatican the Pontiff also prayed that the Church in Timor-Leste may evangelize culture and inculturate the Gospel in the local context so that the faith may translate into the daily life of Timorese Catholics Thank you for reading our article. 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Just click here the defender heavily linked to replace the outgoing Max Kilman in Wolves’ backline this season The position was arguably in need of dire reinforcements at the 2023/24 season’s end after a disastrous 2024 at the back However, this area is now in need of more urgent care after Kilman was shipped off to West Ham This leaves Wolves with only four senior centre-backs at the club going into the new campaign Players have been linked in this position with one consistent name who has popped up being Carmo Molineux News will explain everything you need to know about the defender from his feats to what Wolves may have to pay to acquire his services At the time of writing this makes him 24 years old He also stands at 1.96 metres which is six foot five inches Carmo has only ever played as a central defender He is also left-footed which offers more utility to the club Carmo currently makes his trade at Portuguese giants Porto The defender signed for the club back in 2022 and has since made 27 appearances in all competitions In January 2024, Carmo was sent on loan to Greek side Olympiacos. The signing came in the aftermath of the defender being outcast to Porto B due to “inappropriate behaviour within the workgroup” he played the remaining part of Olympiacos’ season which resulted in the club winning the Europa Conference League in 2024 he elected to play for the Angolan national side where he currently has two caps Carmo excels in plenty of important defensive stats. According to Fbref the defender has 4.42 aerial duels won per game and 1.61 blocks he is completing four progressive passes and 1.49 touches in the opposition box he is seen as a bit of an attacking defender he is having 0.9 shots a game while producing a non-penalty xG of 0.07 Wolves are believed to be interested in Carmo. After Kilman left the club, it was believed that there was a shortlist of four potential replacements, Carmo being one Since then, it has been suggested that Wolves signing Carmo has the most substance. However, it has been reported that Carmo wants to stay at Porto and fight for his spot in the team It is believed that Wolves will need to pay around £15 million if they want to secure the services of Carmo this summer A Brazilian doctor has died after suffering a heart attack as he delivered a child Francisco Xavier do Carmo's medic son Fabricio was among the health professionals who made repeated attempts to try to resuscitate him The drama occurred on Sunday at Nair Alves de Souza Hospital in the city of Paulo Afonso in the north-east Brazilian state of Bahia Footage taken at the hospital after Francisco was declared dead showed colleagues applauding and breaking down in tears as they hugged each other while his body was wheeled out on a stretcher Tributes have been paid to the popular obstetrician today City hall officials in Paulo Afonso said in a statement on their official Instagram site as it declared three days of mourning: 'With great sorrow, the Municipal Council of Paulo Afonso deeply regrets the death of the dear Dr Francisco Xavier do Carmo, a gigantic loss for our entire city. 'Dr. Francisco was not just an exemplary doctor — he was an extraordinary human being, who dedicated his life to caring for others with love, empathy, and commitment. 'Your remarkable presence, your generosity, and your dedication to public health have transformed lives and left an unforgettable mark on our history. 'In this moment of sorrow, we join together with your children Fabricio, Isabela and Talita Xavier, also doctors and collaborators of Nair Alves de Souza Hospital, in extending our solidarity to the entire family, friends, colleagues and everyone who had the privilege of knowing him. 'Out of respect and recognition for his achievements, we are declaring three days of mourning. No further details were revealed about the woman whose child he had been preparing to deliver and what happened to her as the tragedy unfolded. Colleague Adilma Souza said online it had been an honour to have worked with Francisco, saying he loved his work and always treated his medical team to chocolate cake and Coca Cola after surgery. Another said: 'Rest in peace. Francisco always worked out of his love for people.' Banda Amorim wrote in a tribute: 'A great human being who brought sensitive, empathy and care to medicine.' Carlos Alexandre said: 'The best doctor I ever worked with. Rest in peace my dear Francisco Xavier. I have lost a great friend.' No one seems to have shared their thoughts on this topic yetLeave a comment so your voice will be heard first. {{message}} (ANS – Seoul) – The Archbishop of Dili Cardinal Virgílio do Carmo da Silva has visited South Korea to take part in a Congress on migrant workers from Timor-Leste after arriving in South Korea on the morning of 14 September visited the Timor-Leste Embassy in Seoul and was then welcomed at the Salesian Provincial House Accompanying the Cardinal was Ambassador Gregorio de Sousa There are about 6,000 migrant workers from Timor-Leste in South Korea mostly young people in their 20s who work on fishing boats in fishing villages or are employed in rural areas of the country doing simple but very hard jobs The Cardinal shared a warm and fraternal moment with the Salesians once he arrived at the Salesian Provincial House Having already visited South Korea three times - in 2000 when he was Formation Delegate for the Timor-Leste-Indonesia Vice-Province; in 2017 when he was Vice-Provincial and in 2021 when he was ordained Archbishop of Dili - Cardinal do Carmo da Silva has a great interest and knowledge of this country the number of young migrant workers in South Korea has increased dramatically in recent years and he has made great efforts to provide them with pastoral assistance His visit to this country is therefore intended to meet these migrant workers with 65% of its 1.3 million inhabitants under the age of 30 More than 6,000 of these young people are migrant workers in South Korea hundreds of young people leave Timor-Leste for foreign countries such as Australia and South Korea which means that all this is scarce in Timor-Leste." the Salesian Cardinal offered a different perspective with regard to migrant workers from Timor-Leste they are not only people who fill the labour shortage in South Korea but renew the vitality and witness of faith of the local Church The devastation of rural areas has led to the emptying of local churches and it is hoped that migrant workers from Timor-Leste who work in these areas will be able to fill the void for some time and integrate well enough to visibly witness their deep faith in Korean society Cardinal Virgílio do Carmo da Silva had a suggestion of great wisdom for the Korean Church which will also host World Youth Day in 2027 "The young people want to meet the Pope," he said "We bishops were very surprised to see how enthusiastic the young people were about the Holy Father's presence alone." The Archbishop of Dili prayed that the next WYD would be a place of "reconciliation" that it would heal the many wounds in Korea and throughout East Asia "Hundreds of thousands of young people will come from all over the world" he recalled "and if only we could have a party of reconciliation with them.. and I hope that Seoul 2027 will be the centre because there will never be another opportunity like this with young people coming together and playing a leading role in advancing reconciliation They will themselves become messengers of reconciliation and will spread it throughout the world," the Cardinal said Cardinal do Carmo da Silva also expressed his gratitude and best wishes to the Korean Church and the Salesian Family "The Korean Church and the Salesian Family have been generous in helping us in East Timor with various projects and I am grateful that thanks to their support the lives of our people are gradually improving and our hopes for the future are becoming brighter I ask you to continue to demonstrate your spirit of solidarity with humanity and the love of Christ and in particular I ask the Korean Church to help our migrant workers." where the Congress of migrant workers took place Nottingham Forest bring in Porto defender CarmoNottingham Forest have completed the signing of David Carmo from Porto with the defender heading out on loan to Olympiacos for the season.Nottingham Forest have completed the signing of David Carmo from Porto for £10m (€11.7m) with the defender going out on loan to Olympiacos for the season who represents Angola at international level spent the second half of last season in Athens Carmo joins on a five-year deal and is Forest's ninth signing of the summer transfer window He began his career at Braga before moving to Porto for €20m in the summer of 2022 but only managed 27 appearances before being loaned to Olympiacos who are owned by Forest owner Evangelos Marinakis reports emerged on Sunday that Forest's move to sign Arsenal striker Eddie Nketiah had broken down rumoured to be in talks with Feyenoord over Mexico forward Santiago Gimenez Carmo Requejo believes that great design begins with understanding people This core philosophy has guided her two-decade career in design As a mentor for Biosphere at Helsinki Incubators she challenges participants to ground their solutions in real-world needs and user-centred design “Design is not just about aesthetics or function,” she explains “It’s about solving real problems for real people.” Requejo also stresses the importance of empathy and user research from the outset of product development young entrepreneurs are eager to jump into the solution,” she says “But unless you truly understand the people you’re designing for ensuring their solutions are built on genuine need Requejo’s mentorship goes beyond technical guidance or business advice; for her it’s about making meaningful connections with her mentees and addressing both their professional and personal needs.  She recalls instances in her mentoring where some participants faced personal demands which often affected their ability to stay focused on the project “They were stuck because they couldn’t focus,” she says I realised they needed emotional support.” This ability to listen deeply and recognise unspoken needs is a skill Requejo honed through her background in psychotherapy While psychotherapy and design may seem an unlikely combination “Psychotherapy teaches you to look beyond what people say on the surface,” she explains but their real needs are often hidden beneath the surface Uncovering these hidden needs is key to creating impactful As Strategic Design Lead at Nacar Design in Barcelona Requejo’s empathy-driven approach influences her collaborations across industries “We’re not just designing products or interfaces; we’re designing experiences,” she says they must be rooted in understanding people’s needs and behaviours.”  These principles of user empathy and strategic thinking also come as a natural fit for her mentoring approach as she guides her mentees through the nuances of balancing user experience with business goals Requejo’s journey into design was not straightforward she pursued a degree in chemistry but soon realised it wasn’t the right fit “Chemistry required too much imagination for me,” she laughs where I could see the tangible impact of my work.” This led her to industrial design where she found fulfilment in transforming ideas into reality from initial concepts to finished products Requejo has navigated the intersection of design she balances user needs with market demands and business objectives “There’s always a tension between what the business needs and what the users need,” she explains It’s not enough for a product to look good; it must make sense from a business perspective and Requejo achieves this through close collaboration with designers “By bringing different perspectives together we can create solutions that are not only functional but also innovative and impactful.” Requejo is especially passionate about supporting startups in their early stages and aligning solutions with real-world needs “These early stages are critical,” she emphasises “Helping teams approach user research without bias is foundational to their success.” She adds Requejo highlights resilience as essential to entrepreneurship “It takes enormous strength to bring an idea to life,” she says even when the journey becomes difficult.” She often reminds her mentees that setbacks are part of the process and that success rarely comes quickly Requejo advises her mentees to remain adaptable emphasising that initial ideas are just starting points the more your approach may need to evolve.” Sustainability also plays a central role in Requejo’s work and mentorship approach “Designers have a responsibility to create solutions that are not only effective but also environmentally responsible,” she explains “It’s about thinking beyond the product’s lifecycle and considering its impact on the planet.” She encourages budding entrepreneurs to consider the broader implications of their innovations “It’s not just about immediate goals,” she says “Entrepreneurs must consider how their solutions contribute to a more sustainable world I firmly believe that sustainability should be at the heart of innovation.” Requejo is excited about the potential for design to play a pivotal role in building a more sustainable and empathetic world “Design has the power to shape the future,” she says “It’s not just about creating products or solutions—it’s about creating systems and experiences that improve people’s lives while protecting the planet.” Her advice to aspiring entrepreneurs and designers is simple: stay resilient “The world is constantly changing,” she says And the more empathy you bring to your work Home / Somali News Taliyaha Ciidanka booliska dowladda Puntland Muumin Cabdi Shire oo safar shaqo ku yimid degmada Carmo ayaa maanta kormeeray Kulliyadda Tababarka Ciidanka Booliska Soomaaliyeed ee Carmo halkaas oo uu tababar uga socdo dhalinyarada aqoonyahanka ah ee dhammeystay jaamacadaha dalka kuwaas oo ku biiraya Ciidanka Booliska Puntland Taliyaha waxaa si diirran ugu soo dhaweeyay Taliyaha Dugsiga Tababarka ee Carmo, Janaraal Muuse Xasaasi, saraakiisha kulliyadda iyo macallimiinta tababarka Taliyaha Ciidanka Booliska Puntland waxa uu kulamo kala duwan la qaatay madaxda kulliyadda ayaa uga warbixiyay guud ahaan habsami-u-socodka tababarka iyo heerka uu marayo ayaa boggaadiyay shaqada adag ee ay qabteen masuuliyiinta iyo macallimiinta muddadii uu tababarku socday Wuxuu hoosta ka xarriiqay in tababarkani muhiim u yahay dhismaha ciidan aqoon leh oo heegan u ah sugidda amniga iyo ilaalinta sharciga Wuxuu ugu dambeyn ku dhiirrigeliyay dhammaan ka qaybgalayaasha iyo masuuliyiinta in la sii laba jibbaaro dadaalka si loo helo ciidamo tayo leh oo u adeegaya shacabkooda si hufan It’s the end of the road for an Italian stalwart in the Warehouse District This periodic column rounds up restaurant and bar closures in and around New Orleans. Know of a restaurant closing we missed? Drop us a line Tommy’s, a Creole-Italian stalwart in the Warehouse District founded by Tommy Andrade in 2003, will close for good next week, reports the Times-Picayune/New Orleans Advocate. The related New Orleans Social House Both are part of the massive Creole Cuisine Restaurant Concepts group whose CEO Marv Ammari tells the newspaper: “The Tommy’s era is over.” The hospitality group will open two new restaurants with nearby Plates chef (Farrell Harrison) and general manager (Brian Weisnicht) at the helm in those spaces Tommy’s will become a modern French bistro while N.O.S.H will turn into an oyster bar and wine bar projected to open in early 2025 One of the best new bakeries in New Orleans is closing after less than a year, owner Jeremy Fogg announced on Instagram last week Mae’s Bakeshop is the former Emeril’s pastry chef’s ode to his mother and grandmother on Baronne Street a shop that serves classic Southern biscuits feel-good spot that will be missed — Fogg says he has made the “painful” decision to close the storefront possibly leaving room for a return to the pop-up business he previously operated and Thanksgiving pies are still available for preorder The Larder, a popular deli, market, and restaurant in Metairie next to Trader Joe’s, closed earlier this month. Chefs Alison Vega-Knoll and Chris Wilson first opened the cafe in 2020, specializing in breakfast and lunch, grab-and-go prepared food, and catering. Last year, the duo sold it to BRG Hospitality (the company that rebranded from Besh Restaurant Group in 2018) The chefs and owners of Carmo, Dana Honn and Christina do Carmo Honn, announced they are closing their popular tropical restaurant at the end of 2024 after 15 years in the Warehouse District telling Eater the restaurant hasn’t been profitable since the onset of the pandemic and that they’ve been examining how to best transition for the last few years so we have to change course altogether,” says Honn Luckily, that new course includes three separate but related ventures: Nikkei, a Creole izakaya opening in November in the Broadside in Mid-City; Eatwell Kitchen Dragons’ transfer business goes into overdrive After a quiet summer in the transfer market FC Porto made a series of significant moves over the weekend as the new administration led by André Villas-Boas readjusts Vítor Bruno’s squad for the 2024/25 season Tom Kundert rounds up the latest incomings and outgoings at the Estádio do Dragão.  Spain U21 striker Samu Omorodion was a surprise capture from Atletico Madrid is the impending return of midfielder Fábio Vieira David Carmo has been sold and Francisco Conceição has travelled to Juventus with a view to a transfer to the Italian giants The 20-year-old has put pen to paper on a 5-year deal costing Porto 15 million euros for 50% ownership of the player The Dragons have two options to increase their ownership by 15% and a further 15% Omorodion’s buyout clause has been set at 100 million euros “I’m proud to be part of a club with such an enormous history as FC Porto,” he said “I realise the responsibility of playing here where great strikers have played like Fernando Gomes Samuel Omorodion Aghehowa moved to Seville as a child and first started making a name for himself in Granada’s youth system He scored 18 goals in 33 games for Granado B which persuaded Atletico Madrid to buy him last year where he scored eight goals in his first season in La Liga Omorodion will try and fill the gap left by the departure of Porto’s top scorers of recent seasons, Mehdi Taremi who joined Inter Milan after running down his contract, and Evanilson who was sold to Bournemouth last week Swedish U19 International Deniz Gül has also joined Porto for a fee of €4.5 million plus another potential 500,000 euros in add-ons Gül scored three goals in 17 matches for his previous club Hammarby and becomes the third Swedish footballer to play for Porto after Lars Eriksson and Fredrik Söderström Creative midfielder Fábio Vieira is expected to complete a one-season loan move this week. The 24-year-old was sold to Arsenal amid high expectations in 2022 for €35 million but has struggled to make his mark in England in the face of fierce competition for places in the London team as shown in their apparent refusal to sanction an option to buy in the loan deal signing Vieira would be a huge boost for Porto The former Portugal U21 captain was magnificent in the 2021/22 campaign making 23 goal contributions in 39 matches in all competitions as Porto completed a league and cup double Ever since former coach Sérgio Conceição was replaced by his assistant Vítor Bruno in the dugout at the Dragão talk of a move out of the club for his son Francisco Conceição has been incessant Some standout moments at Euro 2024 only added to the rumours and Conceição junior now looks set to sign for Juventus The diminutive winger flew to Turin on Sunday in the company of agent Jorge Mendes with a view to completing the transfer One sale that is confirmed is that of centre-back David Carmo who has joined Premier League club Nottingham Forest for a fee of €11 million plus a possible €4m in add-ons Carmo will not play in England this season however as he was immediately loaned to Olympiacos Both Forest and Olympiacos are owned by Greek businessman Evangelos Marinakis Carmo enjoyed a successful spell at Olympiacos in the second half of last season, helping the Athens club win the UEFA Conference League. Reporting by Gabriel Araujo; Editing by Steven Grattan PETALING JAYA: Malaysia-based online platform iMotorbike is expanding its regional presence but it is doing so with a firm focus on safety Co-founder and CEO Gil Carmo said the used motorcycle market in Malaysia has long been driven by informal sales and unregulated online listings leaving buyers vulnerable to unclear pricing “We aim to address these problems by offering a structured professionally managed marketplace for preowned bikes At the core of its offering is a 170-point inspection process conducted by certified mechanics This includes everything from engine health and suspension condition to ownership documents and service history and would not give them problems a week later We created iMotorbike to fill that trust gap,” he told SunBiz He said every listing that carries the “certified” label has passed this comprehensive check Buyers can view inspection reports online before making a decision removing the guesswork and uncertainty often associated with used vehicle purchases Carmo emphasised that one of iMotorbike’s key selling points is its transparent The platform uses real-time market analytics and historical sales data to ensure that prices reflect true market value especially first-timers unfamiliar with motorcycle pricing while also helping sellers justify their asking price with confidence less doubt and more trust in the transaction,” he said iMotorbike offers a six-day return policy and a six-month warranty on all certified motorcycles a rare feature in the secondhand vehicle space whether it is the ride feel or mechanical performance customers can return or exchange it within six days We also back all certified bikes with a warranty that covers major mechanical and electrical issues “The platform further simplifies the process by bundling road tax and insurance into the purchase offering buyers a more convenient and protected ownership experience.” and iMotorbike addresses this with financing plans tailored to individual profiles “Loan options are designed to be accessible reduce the perceived risk of financing a used vehicle Financing a used motorcycle has historically been difficult and risky We’re changing that by not only providing access but also ensuring that buyers are protected throughout,” Carmo said Following the company’s traction in Malaysia iMotorbike has begun expanding into regional markets “The company has set up local teams and is working with regional partners to align with domestic regulations and consumer habits iMotorbike customises its approach to fit each market’s operational and legal landscape “We do not believe in copy-paste expansion What works in Malaysia might not work in the exact same way elsewhere so we adapt but without compromising on safety or transparency,” Carmo said and increasing digitalisation as major trends shaping the industry “Our mission has always been to make the used motorcycle space more transparent that is the foundation we are building on,” Carmo said Tel: +603-7784 6688      Fax: +603-7785 2625 The ad-free version is ready for purchase on iOS mobile app today we couldn't find that page";var n=e.querySelector("h2");return n&&n.remove(),{staticContent:e,title:t}},d=function(e){var t=document.createElement("button");return t.innerText=e,t.classList.add("error-page-button"),t},f=function(e){var t=document.createElement("div");t.id="recirculation-404",t.classList.add("brand-hint-bg");var n="\n \n \n \n \n \n '.concat(e,' Tick here if you would like us to send you the author’s response The file could not be found for a number of reasons such as the file being moved or deleted. 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Click here to get back to where you came from (ANS – Dili) – "A historic event a celebration for the people of Timor-Leste a celebration of the faith for the Catholic Church in the country": in a conversation with Vatican media the Archbishop of Dili,  Salesian Cardinal Virgílio do Carmo da Silva outlines the first assessment of Pope Francis' visit to the nation which ended on the morning of Wednesday 11 September The cardinal recalls the visit of Saint John Paul II which took place 35 years ago when Dili was the capital of a province of Indonesia and underlines that Bergoglio was the first Pontiff to come to Timor-Leste since the country became independent the Church and all the people have contributed to the success of this visit" stressing that "the strength of the faith in this young country has moved the Pope himself and almost the whole world." focusing on the fruits of the presence of the Pontiff underlining the importance of the relationship between faith and reason that in the last month has also been explored at an academic level and among government leaders at the long "process of building the country the Timorese archbishop remarks how the visit of the Bishop of Rome served to "affirm and confirm the national identity and to tell the Timorese that no matter how small the country is: you are Timorese and you are Catholic and you have your own identity." is that the local population "really becomes rooted in these processes of cultural identity and faith." commenting on the Pope's final speech in Dili – the one given on 11 September  during the meeting with young people at the Centro de Convenções – Cardinal do Carmo da Silva recalls the "two very strong messages" indicated by the Pontiff to the youth: "First: move forward "Both messages are addressed to the people of God" he adds continue to cultivate this attitude in our hearts and respect others then I believe this will be a great step forward to make a difference Source: Vatican News Please select what you would like included for printing: Copy the text below and then paste that into your favorite email application This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply Service map data © OpenStreetMap contributors This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page and Eduardo Menezes of Ross compete in the Suffolk boys tennis small school finals on Friday It was less than a week ago that Ross’ Eduardo Menezes rolled through the field to become Suffolk County’s boys tennis singles champion He was told Friday morning there would be a new play for that day’s county small schools championship match against Bayport-Blue Point — he would be paired with Leonardo Carmo at first doubles The chess match that is team tennis was on Menezes and Carmo had never played together but adapted quickly and defeated the Phantoms’ Shane Duerr and Matt Decatur in what proved a critical victory as Ross defeated Bayport-Blue Point The first four matches to finish were all Ravens victories 6-1 win at third singles being the clincher Ross (15-0) is county champion for a second straight year and will be seeking its first Long Island crown on Tuesday against Nassau champion Friends Academy at Smithtown East It’s a rematch of the 2023 Long Island championship “Friends had amazing players and that day they were better We’re more prepared this time and we have a team that can win it.” The Ravens’ three singles entries — Henry Tietz Jagger Cohen and Brodlieb — lost an aggregate three games in winning six sets BBP got doubles wins from the teams of Aidan Apicella and Nicholas Byman Luke Jensen and Dom Linzie and Declan Schug and Eric Swinkin “This ended up close but this team is still hungry,” Ravens captain Alex Frohlich said Added Cohen: “This team has wanted a shot at Friends all season — you hear it daily.” Ross’ recent rise to Suffolk tennis prominence has come with a number of lessons the Ravens have been laden with talent but occasionally been outmaneuvered by teams that spread their singles talent through the doubles teams I’m doing some things that have been done to us Ross is a private school and not only has local students but also ones from Germany Like in other parts of the state where parochial schools play in the public leagues “I have a tremendous amount of respect for the Ross tennis program — not only do these coaches put an incredible product on the court but these players handle themselves with class and integrity off the court as well,” Bayport-Blue Point coach John Selvaggio said “But I am willing to say the quiet part out loud The Ross School represents three continents and Bayport-Blue-Point represents three square miles This will be the second year in a row that the Long Island Region of the New York State Public School Tennis Championship will be represented by two private schools The Friends Academy boys tennis team dropped only two sets over five matches as it rolled past Wheatley to a 5-0 victory in the final of the Nassau Small Schools Championships at Hofstra Will Bohner rallied from losing the first set to score a 3-6 Bryan Bin and Hudson Lee stumbled early as well but recovered for a 3-6 Friends Academy (7-9) is county champion for a second straight year and is seeking a second straight Long Island title against Ross Tuesday “This team has really started to mesh together with the higher stakes this past week,” Quakers coach Owen Kassimir said “The match against Ross is going to be a fight Russell Notaris dropped only one game in winning his singles match and the doubles teams of Zack Cohen and Danny Duke and Vedant Rawat and Henry Koelmel both won their matches without dropping a set The Newsday app makes it easier to access content without having to log in Unlimited Digital AccessOnly 25¢for 6 months