READ MONOGRAPH Francisco Granja de Almeida is the new head of legal at C-More a Portuguese technology company specializing in ESG software He holds a bachelor’s degree in Law from the University of Lisbon and an LL.M in Public International Law from Utrecht University where he served as legal counsel and human rights specialist Francisco brings a strong academic background he will help grow our ESG-legal team in Portugal and drive new business” Almeida worked as an associate in social economy and human rights at Vieira de Almeida a legal and compliance officer at Novartis and a paralegal at Jorge Neto Valente – Lawyers and Notaries in Macau Caiado Guerreiro announces two new partners It represents the main source of information in the legal business sector in Spain and Portugal The digital magazine – and its portal – address to the protagonists of law firms and in-house lawyers The magazine is available for free on the website and on Google Play and App Store information about deals and their advisors For further information, please visit the Group’s website www.lcpublishinggroup.com Family-owned Granja Pocha produces items such as cheese and yogurt under its Colonial brand Lactalis plans to buy the Uruguay-based dairy company Granja Pocha The French dairy giant said in a statement it will buy all of the shares in family-owned Granja Pocha which produces items such as cheese and yogurt under its Colonial brand Granja Pocha manufactures its products at a facility in Juan Lacaze Lactalis Uruguay will expand its local market position and an additional 60 million liters of milk will be processed into dairy products complementing Lactalis Uruguay’s existing offer of cheese fluid milk and milk powder,” a spokesperson for the French dairy business told Just Food which is subject to approval by Uruguay’s competition authority Lactalis added in the statement that the acquisition of Granja Pocha will “contribute to enhancing the company’s sustainability in the medium and long term” Don’t let policy changes catch you off guard Stay proactive with real-time data and expert analysis all of whom will be retained post-transaction Director of the Uruguayan food producer Edgardo Villanueva and his family will continue to “collaborate” after the move The French dairy producer already has a presence in Uruguay with a factory in Cardona which includes a drying tower that will complement production at the Granja Pocha plant in Juan Lacaze Granja Pocha also produces dairy desserts and dulce de leche a caramel-based sweet treat popular in Latin-America Nominations are now open for the prestigious Just Food Excellence Awards - one of the industry's most recognised programmes celebrating innovation This is your chance to showcase your achievements Don't miss the opportunity to be honoured among the best - submit your nomination today Give your business an edge with our leading industry insights View all newsletters from across the GlobalData Media network It’s Barcelona the word means “farm,” but it also eventually came to refer to businesses that sold milk It’s also one of the city’s most progressive Realizing the sanitary downsides of having cows in their increasingly urban backyard the early owners bought a farm at the city’s edge and created a small factory that made cheeses and other dairy products that could be brought into the city they were forerunners of milk preservation and packaging allegedly the world’s first bottled chocolate milk Viader retains an Old World feel with ancient floor tiles and vintage furniture and signage; the plaque out front indicates that it’s been recognized by the city for these elements They serve what many consider Barcelona’s classic crema Catalana Catalonia’s silky cousin to crème brûlée.  made aromatic by the addition of lemon zest Friendly staff will coach you through the process of breaking the glassy crust with your spoon and taking a spoonful that includes both the custard and those crispy shards Another Catalonia-specific item available here is mel i mató cottage cheese served drizzled with honey and topped with walnuts Viader closes for a siesta between 1:30 p.m Savor sheep milk yogurt at the last of Athens' dairy bars are reborn here with a glassy sugar crust and a center as light as whipped cream there's always room for dessert—it's just upstairs Travel to the Balearics without leaving Barcelona at this restaurant specializing in Mallorcan cuisine Louis has been serving up treats for more than a century This old-school soda shop is the home of the Tin Roof Sundae This family-owned canteen has been selling goat milk ice cream since 1933 Partes de esta página pueden haber sido traducidas automáticamente El servicio de Extensión de Oregon State University (OSU) no garantiza la exactitud de ningún texto traducido automáticamente Consulte la versión original en inglés para confirmar la información Oregon’s Farm Direct Marketing Law allows farmers to turn what they grow into low-risk value-added products like jams and pickles and to sell them directly to consumers without being licensed food processors That sentence — like the law itself — has a lot of detail packed into it This short guide unpacks those details for farmers and farmers market managers too.) Use this flow chart to determine whether a product qualifies for Farm Direct sales This flow chart illustrates how to determine whether a product qualifies for Farm Direct sales Make sure the product label has all the required info Acidic fruit and vegetable juices that have not received a 5-log reduction of the most resistant microorganism of public health significance must include the following warning statement: WARNING: This product has not been pasteurized and therefore may contain harmful bacteria that can cause serious illness in children the elderly and persons with weakened immune systems Sales records are required for all value-added products sold under the exemption Sales records must include the product(s) sold quantity sold and a current rolling total of year-to-date sales Additional records are required for each batch of acidified foods These records must include the recipe source and pH meter calibration records must be maintained Additional processing and production records are required for dried or cured fruits for which drying or curing is not part of routine post-harvest handling Recording water activity (Aw) is not required Copies of all records must be maintained and kept available for the Oregon Department of Agriculture for a period of three years pH meter calibration records and equilibrium pH measurement records are required for each batch of acidified food colorimetric methods such as litmus paper may be used pH litmus paper may not work well with some colored products If the pH of the product is between 4.0 and 4.6 a pH meter with a minimum accuracy of ± 0.01 to 0.02 pH must be used Naturally acidic foods like fruit jams and jellies are not required to be pH tested farmers can take pH measurements to verify that the product is acidic and products made from them should be pH tested to ensure safety See video instructions for pH testing an acidified food at pH Meter Calibrating and Testing for Pickled Food Products. If you are unsure your product qualifies, contact the Oregon Department of Agriculture Food Safety Division. All products must be grown and processed by the farmer The following products are also allowed as long as they are prepared on-site not intended for immediate consumption and are packaged to take home: ODA recognizes the following references as providing approved processes and formulations (recipes) for acidified products sold under the Farm Direct exemption: Farmers may also submit a copy of the recipe — including the product ingredients list, directions, formulation, and thermal processes used — to a recognized authority for approval. Contact the OSU Department of Food Science and Technology for more information Value-added products not covered under the Farm Direct exemption — including products made from recipes not listed by an approved source or with ingredients not grown by the farmer — must be made in a licensed facility There are four options for processing in a licensed facility For more information about licensing requirements for domestic kitchens and commercial facilities, and to find your local ODA food safety specialist, see the ODA Food Safety Program This document should be regarded strictly as a supplement to the Oregon Farm Direct Marketing Rules and should never be considered a substitute to reading and understanding the regulations. For a complete list of requirements, please consult OAR 603-025-0215 through 603-025-0275 Cómo usar esta página Court records show Christopher Granja was booked Wednesday on a count of vehicular homicide as well as driving without a license causing death The probable cause affidavit against Granja notes he admitted to driving the blue Hyundai Sonata shortly after midnight on Monday when the car slammed into a light pole just north of Presidential Golfview Condominiums The impact of the collision toppled the light pole and a 25-foot portion of it came down on the backseat of the sedan Granja and the person in the front seat were suffered minor injuries in the wreck he claimed to be going between 40 and 50 mph at the time they approached the entrance to the condominiums Granja is reported to have said he saw a truck leaving the complex and swerved to avoid it when he lost control He said that he closed his eyes and missed the truck before losing control of the car revealed the estimated deceleration before the crash and the force of the impact indicated Granja’s car was likely going around 80 mph right before the wreck which is double the speed limit in that area Investigators stated they could find no evidence that supported Granja’s claim about a black truck being in the area right before the crash Granja also admitted he did not have a license WPBPD noted that the Sonata had belonged to Robertson’s grandmother Granja was given the keys because he was the better driver both Granja and the person in the passenger seat told investigators Find more ways to stay up to date with your latest local news. 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Subscribe to our YouTube channel for the biggest stories and can't miss video Granja Pinilla has more than one million hens and sells to supermarket chain Mercadona Italian family-owned egg producer Eurovo has taken over the Spanish egg business Granja Pinilla in its entirety has three production and classification centres in Megeces employs 80 people and turns over more than €40mn ($43.7m) according to local business news site Revista Info Retail It counts domestic supermarket chain Mercadona among its clients and also produces eggs for export The deal was confirmed to Just Food by Granja Pinilla In a statement carried by Revista Info Retail Eurovo CEO Ciro Lionello said Spain is an attractive market for investment “The entry of Granja Pinilla into our group represents an important strategic step Spain is a rapidly growing market and being able to count on a solid company with a long family tradition and a reputation based on quality reliability and attention to animal welfare makes us proud,” he said Granja Pinilla said “Granja Pinilla will continue to grow within the Eurovo Group thanks to sharing the same corporate and family values and the same strategic vision We are proud to have reached this important agreement for the company and its employees,” Granja Pinilla said The purchase of Granja Pinilla comes in the same week as Eurovo’s deal to buy a majority stake in UK egg whites business Two Chicks Founded in 2007 by Anna Richey and Alla Ouvarova Two Chicks claims to be the “first” to launch liquid egg white in UK supermarkets and has doubled its turnover over the past two years Eurovo posted annual revenues of around €1.25bn ($1.38bn) in 2024 and supplies approximately 5,000 customers across more than 40 countries The company’s portfolio includes consumer brands Le Naturelle the company operates 28 sites across Europe These facilities span markets including France a leading European producer and distributor of eggs and egg-based products has acquired 100% of the shares of the Spanish company Granja Pinilla This acquisition consolidates Eurovo’s presence in the Spanish market and further strengthens its position in the industry Granja Pinilla is a company from Valladolid with more than seven decades of experience in the egg sector dedicated to the production and marketing of chicken eggs The company has a fully integrated structure controlling the entire production process: from poultry breeding and feed production to the selection and packaging of the final product The company currently operates three production and classification centers located in Megeces where it houses more than one million hens Its staff is made up of 80 professionals and its annual turnover exceeds 40 million euros Eurovo was assisted by Deloitte Legal through its M&A team led by partner Massimo Zamorani and by the Spanish team including partner Agustín del Río senior associate Carlos Reverter Rodriguez-Marques Tax advisory was provided by a dedicated M&A tax team: for STS Deloitte the team was led by partner Matteo Cornacchia with manager Fabio Casucci Durán-Sindreu grows after the integration of Auxia Abogados Photos: Granja FariaRicardo Castellar de Faria a 49-year-old entrepreneur and owner of Granja Faria has become the latest Brazilian to join the prestigious Forbes list of billionaires Faria now holds the 21st position on the Forbes Brazil ranking with an estimated net worth of U$3.11 billion was born in Rio de Janeiro but built his fortune in Santa Catarina where his family relocated during his childhood His entrepreneurial journey began when he was 7 years old with a small ice cream cart business now the largest commercial egg producer in Brazil and one of the biggest in Latin America Granja Faria was established in 2006 in Nova Mutum and has since grown into a powerhouse in the poultry industry The company produces 16 million eggs daily and employs 2,700 people across 34 production units Granja Faria is also a leader in free-range chicken farming maintaining a flock of approximately 2 million birds The company produces commercial and free-range eggs Faria is the president of the Associação Catarinense de Avicultura (ACAV) ALSO READ: Brazil’s corn industry is discovering DDGS His ventures have since expanded beyond poultry – 4 years ago a company specialising in agricultural management for rural properties All of these enterprises are grouped under the holding RCF Capital Granja Faria has also made headlines for its corporate social responsibility initiatives such as donating 1 million eggs to flood victims in Rio Grande do Sul in May which shows the photographer's personal vision of the fountains at the Royal Site of La Granja de San Ildefonso is on display from today until December 15th at the Royal Palace of La Granja visitors can enjoy the exhibition’s 24 images in the context in which they were taken while understanding more deeply the landscape and engineering value of the ornamental fountains in the palace which celebrates its 300th anniversary this year awed by the music of the waters that descend more than a thousand meters from the peaks of Peñalara nourishing this ecosystem and powering the hydraulic system of the three-hundred-year-old gardens of La Granja de San Ildefonso,” the artist explained at the project’s launch in Madrid last June has already been displayed in Madrid's Jardines del Moro where these photographs can still be enjoyed until September 29th This exhibition is part of the three-year project titled Fieldnotes PHotoESPAÑA and Spain’s National Heritage Agency collaborate to encourage major national photographers to highlight the natural heritage of Spain’s Royal Sites Spain’s National Heritage Agency and ACCIONA already collaborated in 2022 when they jointly promoted the exhibition titled Sebastião Salgado and the Royal Collections: encounters around landscape photography ACCIONA and PHotoESPAÑA have been collaborating since 2017 to jointly give visibility to an artistic look at the human impact on nature and natural resources ACCIONA sponsored the exhibition titled Edward Burtynsky: African Studies ACCIONA and PHotoESPAÑA have also worked together on some emblematic exhibitions such as Isabel Muñoz's We’re Water (Somos Agua) which was displayed at the Lázaro Galdiano Museum and became one of the most visited exhibitions of the 2021 edition and the S.M.A.R.T photographic and audiovisual exhibition which was one of the main activities of the 2019 edition The collaboration between both entities was not interrupted in 2020 when they teamed up to promote the #PHEdesdemibalcón contest in the 2020 virtual edition which revealed the nature emerging in urban environments emptied by the COVID19 outbreak PHotoESPAÑA is a photography festival that has been held annually in Madrid since 1998 and has set an international benchmark in the visual arts for the quality of artworks and artists in the official section as well as for the cultural initiatives it promotes to disseminate the cultural and aesthetic value of photography in society I accept Information on data protection In compliance with Regulation (EU) 2016/679 on Data Protection and with other Data Protection regulations in force you are hereby informed that your personal data shall be processed by Acciona whose identification data are as follows: Tax ID No (NIF): A08001851; Address: Avenida de la Gran Vía de Hortaleza No.: +34 91 663 28 50; email: protecciondedatos@acciona.com Your data shall be processed in order to send you information through the subscription to our Newsletter through electronic means activities and news pertinent to our activity sectors The consent given by the data subject by indicating that they have read and accept this data protection information comprises the lawfulness of processing the subscription Request cannot be satisfied We may also process your satisfaction or preferences Data shall be stored until the elimination is requested we may 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Furthermore, at any time the data subject may withdraw the granted consent by contacting the aforementioned address and file a claim to the Supervisory Authority (Agencia Española de Protección de Datos www.aepd.es) You can also unsubscribe from the Newsletter communication For any further information you can visit the Privacy Police on the website https://www.acciona.com/privacy-policy/ Canadian miner First Quantum Minerals expects updated drilling results for its Peru copper project La Granja in the second half of next year project development manager Steven Lewis said on Thursday and we expect to have the results in the second half of next year,” Lewis said on the sidelines of a mining industry conference About the feasibility studies slated for completion by 2028 he said: “We’re working to meet this deadline.” First Quantum last year purchased a 55% stake in the project and at the time of the acquisition committed to investing up to $546 million for its development The project overall is estimated to amount to $2.4 billion investment According to data from Peru’s mining ministry the site has the potential to produce 500,000 metric tons of copper a year In addition to completing drilling and feasibility studies First Quantum will also face challenges related to local communities “La Granja has the potential to be a larger project but the ultimate focus will depend on several challenges,” Lewis said in a presentation at the mining event First Quantum took a major blow last year after the Panama government ordered the shutdown of the Cobre Panama mine which contributed to 40% of the miner’s revenue due to public protests over environmental issues (By Marco Aquino and Daina Beth Solomon; Editing by Aida Pelaez-Fernandez) and website in this browser for the next time I comment Δdocument.getElementById( "ak_js_1" ).setAttribute( "value" Gruyere gold mine joint venture partners Gold Fields and Gold Road Resources reach agreement on a friendly deal to consolidate ownership. The initiative will be delivered through the regional joint venture established by Fleet Space Technologies and Tahreez. The US central bank is widely expected to hold rates steady in this meeting. Romania has major reserves of rare earths, gold and copper, which have attracted interest from Canadian and American firms. Copyright © 2022 ALM Media Properties, LLC. The plaintiff, Leader Entertainment S.A., is represented by Elio F. Martinez, a shareholder at GrayRobinson, in the action against the defendants, Florida companies Crom Productions, Anclas Productions, and Claudio Berraud. A renowned Argentine production company has filed a trademark infringement lawsuit in a federal district court in Miami against multiple entities over their alleged production and showcasing of a series of live children's programs titled "La Granja en vivo" in Florida Litigation Daily National Law Journal The Recorder New York Law Journal The Legal Intelligencer Brugh Lower of Gibbons has entered an appearance for industrial equipment supplier Devco Corporation in a pending trademark infringement lawsuit accusing the defendant of selling knock-off Graco products 18 in New Jersey District Court by Rivkin Radler on behalf of Graco Inc Read More Yalowitz of Arnold & Porter Kaye Scholer have entered their appearances for Hanaco Venture Capital and its executives 24 in New York Southern District Court by Zell accuses the defendants of negligently and fraudulently managing the plaintiff's $1 million investment Read More Attorneys from A&O Shearman has stepped in as defense counsel for Toronto-Dominion Bank and other defendants in a pending securities class action 11 in New York Southern District Court by Bleichmar Fonti & Auld accuses the defendants of concealing the bank's 'pervasive' deficiencies in regards to its compliance with the Bank Secrecy Act and the quality of its anti-money laundering controls Read More a Pennsylvania company providing shared communications infrastructure Wolf of Gordon Rees Scully Mansukhani to fend off a pending breach-of-contract lawsuit 25 in Michigan Eastern District Court by Hooper Hathaway PC on behalf of The Town Residences LLC accuses Crown Castle of failing to transfer approximately $30,000 in utility payments from T-Mobile in breach of a roof-top lease and assignment agreement Read More Schwartz of McCarter & English have stepped in as defense counsel to Electrolux Home Products Inc 26 in New York Eastern District Court by Poulos Lopiccolo PC and Nagel Rice LLP on behalf of David Stern alleges that the defendant's refrigerators’ drawers and shelving repeatedly break and fall apart within months after purchase Read More Already have an account? Sign In Sixty-eight Honduran shrimp farmers from Grupo Granjas Marinas (GGM) have recently completed a training course as part of an initiative to improve the welfare of shrimp sold in UK supermarket M&S The training was conducted by FAI, as part of their shrimp welfare project which was born out of a collaboration with several industry partners scientists and shrimp farms aiming to help farmers monitor and implement welfare improvements For GGM, it is one of the continuous improvement activities undertaken within its partnership with global aquaculture company Sea Farms and leading UK retailer M&S to secure a better quality of life for shrimp Operating in more than 5,000 hectares and harvesting 18,000 tonnes of vannamei shrimp annually GGM controls all aspects of its shrimp production in a fully integrated system Those attending the training represented all parts of the production cycle from farm managers to the feeding and harvesting teams health and quality assurance staff and wider field workers reflected on the workshop: “The GGM team has strong knowledge of shrimp farming and shrimp needs The workshop gave the team confidence they are taking good care of their shrimp and provided the tools to record and report the outcomes of their good practice.”  added: “The consumer does not necessarily understand what high welfare shrimp farming looks like but FAI’s assessment process and scoring system helps decode our farming data into understandable measures indicating successful production from an animal welfare perspective.” stating: “We are delighted to have implemented a standardised approach to assessing welfare This system helps the farm team identify where changes are needed and provides the right information to the rest of the supply chain so the final consumer is assured of the ethical responsibility behind the product they are buying as well as the quality.” Manage My Cookies The failures of Silicon Valley Bank and First Republic Bank in 2023 caused plenty of finger-pointing at bank regulators Many US financial institutions including SVB and First Republic were covering large portions of their liabilities with assets such as government bonds that stood to lose value if interest rates rose significantly—which is exactly what happened As the Federal Reserve hiked interest rates rumors swirled that the two banks might not have sufficient assets to cover all their deposits Indiana University’s Yadav Gopalan and Chicago Booth’s João Granja find through some rough calculations that had regulators acted two quarters earlier they would have averted $9 billion in losses the researchers also say their analysis of the events reveals a more complicated picture than examiners being oblivious to systemic risks—plus it’s particularly hard to assess regulators’ performance given how necessarily secretive their work is “Regulators may receive the blame for some very public failures but no glory for the bank failures that they prevented from happening,” write Granja and Gopalan the two researchers matched information maintained by banking regulators with publicly available data about banks’ financial conditions show the risk scores supervisors assigned to the banks while the publicly available data include factors such as interest-rate risk exposure US bank regulators assess six categories and issue what’s known as a CAMELS rating regulators most frequently downgraded the “Liquidity” and “Sensitivity to market risk” components of CAMELS This suggests that they understood the increased risks associated with higher interest rates The onset of interest-rate hikes in early 2022 served as a natural laboratory for assessing how bank examiners managed a credit shock the Federal Reserve’s decision to raise interest rates triggered a shock to the value of equity of banks with significant maturity mismatches and unstable deposits,” write the researchers regulators issue what’s known as a CAMELS rating The acronym refers to the six categories that are assessed: capital adequacy regulators adjusted the ratings they issued to the most-exposed banks downgrading two of the six risk factors the most: liquidity and sensitivity to risk A rate shock naturally draws more attention to these components than the others so the downgrades suggest that supervisors “understood the consequences” of interest-rate risk Examiners were less likely to downgrade banks that had derivative contracts that protected them against rate movements another indication that they understood the emerging risks examiners were more likely to downgrade banks with larger unrealized losses in AFS securities but only began to significantly downgrade banks with HTM portfolio losses during the first quarter of 2023 “This inaction of supervisors may be surprising,” Gopalan and Granja write it also may confirm supervisors’ own admissions in the wake of the fallout of SVB that current supervisory models don’t go far enough to capture the additional liquidity risks associated with an unstable deposit base It’s clear that regulators did catch and contain some of the risks saving money for taxpayers and bank customers alike But considering the steep costs of delayed action it’s fair to question whether the failure to detect a fairly straightforward risk—and move quickly to stop it—indicts the entire supervisory system and the $2 billion a year it costs taxpayers which lost the right to operate its copper mine in Panama last year following protests by environmental groups and a court ruling deeming its contract void is seeking to speed its copper projects in Peru One of the three largest global copper producers First Quantum in Peru controls the La Granja project “These are projects that need to be accelerated,” said the mining company’s project development director where fellow miner Rio Tinto is a minority partner is a project with a 40-year lifespan and forecasted output of 500,000 metric tons per year First Quantum’s Lewis said it has one of the largest undeveloped copper deposits in the world fully owned by First Quantum in the Apurimac region is in the “pre-feasibility” stage It could reach an annual output of 200,000 tons once at full capacity “We are very busy building more positive relationships with the communities (in Haquira) to allow it to come to light,” said Lewis who did not offer a time frame for the construction of both mines in the South American country The company shifted focus toward Peru after Panama’s government decided last year to annul First Quantum’s contract to operate the Cobre Panama mine there which accounted for about 40% of First Quantum’s revenue last year “We are now working with Panama’s government to ensure the environmental stability (of the project) the safety of our employees,” Lewis said Panama’s outgoing government of President Laurentino Cortizo ordered the closure of the mine which is currently in maintenance mode while the formal closure process starts The incoming administration of President-elect Jose Raul Mulino will be tasked with setting guidelines for the process (By Marco Aquino; Editing by David Gregorio) Read More: Panama president-elect rules out First Quantum talks until arbitration dropped wildlife and adventure water park in the outskirts of the city of Murcia Terra Natura Murcia is one of the most important and spectacular animal and nature parks in western Spain and is one of a new generation of wildlife parks in which visitors can discover the concept of what's called "Zooinmersión." The aim of this is recreate a habitat which gives an experience of being at one with the wildlife viewed in the park so to this end more than 500 examples of 50 species of vegetation have been interplanted among the animal habitats spread across an area occupying more than 165,000 square metres The planting has matured to create a comfortable and interesting environment which enhances the interaction between visitors and animals The planting not only softens the landscape but also enables visitors to be closer to the animals and have direct human contact rather than the traditional forms of wildlife park where the animals are at a distance behind physical barriers The park contains over 500 animals belonging to 50 different species (some of which are in grave danger of extinction) Iberian wolves and a White African Rhinoceros Terra Natura Murcia offers different educational talks during visits to their facilities: giraffes One of the most popular areas is the falconry zone where the displays enable visitors to discover the beauty of a great variety of birds such as the majestic flight of the peregrine falcon which reaches diving speeds of over 300 km an hour One of the main aims of the park is to encourage contact and engage in interaction between animals and humans and the falconry is a popular means of achieving this goal Among the mammals are Iberian and European Lynx During the summer Terra Natura opens Aqua Natura the only water park in the Region of Murcia This is a refreshing and enjoyable way to cool down in the heat of summer with swimming pools for adults and children all within just a short distance of the centre of the city of Murcia Terra Natura Murcia is open every day of the year except 1st January and 25th December The wildlife park opens at 10.00 in the morning and during the summer (June 23rd to September 8th) it opens at 11.00 The water park opens from May 25th to September 8th January 2nd – March 15th: 17.00 except weekends in February and March (18.00) June 17th – September 8th: 20.00 NB: During the summer special nocturnal tours and meals are available See what's on section for latest promotions September 9th – September 15th: 19.00 September 16th – October 25th: 18.00 except weekends in September and October (19.00) Details of daily activities can be found here: extra activities and special offers are regularly updated on Murcia Today Click here for online bookings Terra Natura is located in the northern outskirts of the city of Murcia in Espinardo Address: Calle Regidor Cayetano Gago, 30100 From the Autovia A-7 Alicante to Murcia Bus line number 31 leaves from Plaza Cruz Roja via Gran Via and passes by the park from Monday to Saturday every half hour and on Sunday every hour leaves from the railway station from Monday to Friday every 15 minutes Line 39C leaves from Infante Juan Manuel (Pio Baroja) from Monday to Friday every 30 minutes and thank you for choosing CamposolToday.com to publicise your organisation’s info or event Camposol Today is a website set up by Murcia Today specifically for residents of the urbanisation in Southwest Murcia providing news and information on what’s happening in the local area which is the largest English-speaking expat area in the Region of Murcia When submitting text to be included on Camposol Today please abide by the following guidelines so we can upload your article as swiftly as possible: Send an email to editor@camposoltoday.com or contact@murciatoday.com Attach the information in a Word Document or Google Doc Also attach a photo to illustrate your article Get Better Academy posted the news of Granja's commitment on social media which Granja reposted on his Instagram page.  "I am so excited to be joining the Memphis program It's a dream come true," Granja told international basketball reporter Riccardo De Angelis.  The 19-year-old spent the past year playing for Get Better Academy in Prague where he was a member of GBA Sojky Pelhrimov (part of 1 Liga a professional league in the Czech Republic) and the U19 Czech league.  Granja averaged 9.1 points (38.4% from the field and 25.6% from three) and 3.3 rebounds in 19 games with GBA Sojky Pelhrimov. In 19 games with the Czech Republic U19 League Granja averaged 13.7 points (43.1% from the field and 30.7% from three) and 4.3 rebounds MEMPHIS BASKETBALL MAILBAG: What we know about Alex Lomax, who Penny Hardaway might sign next GROUP CHAT: Sign up for the Memphis Tigers Basketball Insider text group with Jason Munz Granja played for FC Barcelona in his home country for two seasons He averaged 8.3 points per game in the 2020-21 regular season and 9.0 per game in Spain U18 Club Champions League competition.  Granja becomes the only freshman on the Tigers' roster although Hardaway still has at least one more open scholarship remaining.  Memphis concluded the second (and final) session of summer workouts this week the Tigers will return to campus when classes start Aug Reach sports writer Jason Munz at jason.munz@commercialappeal.com or on Twitter @munzly Roxana Amed is an Argentine-American jazz artist born in Buenos Aires based in the USA who has been nominated for a Latin Grammy is a member of NARAS & LARAS and a Gardel Award winner She is a singular singer-songwriter whose music blends South American folk traditions with art rock and modern jazz Amed has been praised by Grammy.com for her “artistic vision and understanding of her place in the canon” audiences and critics as one of the most important voices in South American music She is currently a teacher at the Frost School of Music and has earned acclaim for her albums with fellow Argentine multi-instrumentalist and former Pat Metheny band member Pedro Aznar including Limbo (2004) and Entremundos (2006) a duo album with pianist Adrián Iaies (Latin Grammy nominee) followed by La Sombra de Su Sombra (2013) with pianist and composer Frank Carlberg featuring the poems of Alejandra Pizarnik she released Ontology which featured her group led by pianist Martin Bejerano and her interpreting songs by Wayne Shorter It had a significant impact on the international jazz media scene collecting excellent reviews her album Ontology received two Latin Grammy Awards nominations in the Best Latin Jazz/Jazz Album and Best Arrangement categories this album won the Gardel Award for Best Jazz Album in 2022 in Argentina In 2023 she received a new nomination for the Gardel Awards for Argentine music and a Latin Grammy Nomination for Best Latin Jazz/Jazz Album for her album Unánime which once again received distinguished criticism on the American On 9 June 2023 she released the album Los Trabajos y Las Noches with composer/pianist Frank Carlberg second part of a project based on music written for poems by the great Argentine writer Alejandra Pizarnik It received 4 stars in the jazz magazine ‘Downbeat’ and was successfully presented in New York last August Her new album Becoming human is the result of a New Jazz Works grant by Chamber Music America 43,000+ global companies doing business in the region 102,000+ key contacts related to companies and projects news and interviews about your industry in English Metrics details An Author Correction to this article was published on 31 March 2021 This article has been updated The advent of single-cell chromatin accessibility profiling has accelerated the ability to map gene regulatory landscapes but has outpaced the development of scalable software to rapidly extract biological meaning from these data. Here we present a software suite for single-cell analysis of regulatory chromatin in R (ArchR; https://www.archrproject.com/) that enables fast and comprehensive analysis of single-cell chromatin accessibility data user-focused interface for complex single-cell analyses single-cell clustering and cell type identification mRNA expression level prediction from chromatin accessibility and multi-omic integration with single-cell RNA sequencing (scRNA-seq) Enabling the analysis of over 1.2 million single cells within 8 h on a standard Unix laptop ArchR is a comprehensive software suite for end-to-end analysis of single-cell chromatin accessibility that will accelerate the understanding of gene regulation at the resolution of individual cells a crucial requirement that would facilitate the broad use of these methods for investigating gene regulation at cellular resolution Schematic of the ArchR workflow from pre-aligned scATAC-seq data as BAM or fragment files to diverse data analysis Comparison of runtime and memory usage by ArchR Signac and SnapATAC (Snap) for the analysis of ~20,000 PBMCs using 32 GB of RAM and eight cores (b) or ~70,000 PBMCs using 128 GB of RAM and 20 cores (c) Dots represent replicates of benchmarking analysis (n = 3) Initial UMAP embedding of scATAC-seq data from two replicates of the cell line-mixing experiment (n = 38,072 total cells from ten different cell lines) Schematic of doublet identification with ArchR colored by the enrichment of projected synthetic doublets (f) or the demuxlet identification labels based on genotype identification using single-nucleotide polymorphisms (SNPs) within accessible chromatin sites (g) ROC curves of doublet prediction using ArchR doublet identification or the number of fragments per cell compared to demuxlet as a ground truth The AUCs for these ROC curves are annotated below UMAP after ArchR doublet removal of scATAC-seq data from two replicates of the cell line-mixing experiment (n = 27,220 doublet-filtered cells from ten different cell lines) colored by demuxlet identification labels based on genotype identification using SNPs within accessible chromatin sites the identification and removal of heterotypic doublets in ArchR reduces false cluster identification and improves the fidelity of downstream results the efficiency of the iterative LSI implementation in ArchR limits the requirement for this estimated LSI approach to only extremely large datasets (>200,000 cells for 32 GB of RAM and eight cores) whereas estimated LDM approaches are required for comparatively smaller datasets (>25,000 cells for 32 GB of RAM and eight cores) in SnapATAC ArchR therefore has the ability to efficiently analyze both large- and small-scale datasets a, UMAPs of scATAC-seq from PBMCs (top) and bone marrow cells (bottom), colored by aligned scRNA-seq clusters. This alignment was used for benchmarking of scATAC-seq gene score models. A list of abbreviations used in this figure appear in the Methods Heatmaps summarizing the accuracy (measured by Pearson correlation) across 56 gene score models for both the top 1,000 differentially expressed (diff exp.) and the top 2,000 variable genes for both PBMC and bone marrow cell datasets Each heatmap entry is colored by the model rank in the given correlation test as described below the heatmap The model class is indicated to the left of each heatmap by color which uses bi-directional exponential decays from the gene TSS (extended upstream by 5 kb) and the gene transcription termination site (TTS) while accounting for neighboring gene boundaries This model was shown to be more accurate than other models Side-by-side UMAPs for PBMCs and bone marrow cells colored by gene scores from model 42 (left) and gene expression from the scRNA-seq alignment for key immune cell-related marker genes (right) Heatmaps of gene expression (top) or gene scores for the top 1,000 differentially expressed genes (bottom) (selected from scRNA-seq) across all cell aggregates for PBMCs (e) or bone marrow cells (f) Color bars to the left of each heatmap represent the PBMC or bone marrow cell cluster derived from scRNA-seq data Runtimes for ArchR-based analysis of over 220,000 and 1,200,000 single cells using a small-cluster-based computational environment (32 GB of RAM and eight cores with HP Lustre storage) and a personal MacBook Pro laptop (32 GB of RAM and eight cores with an external (ext.) USB hard drive) Color indicates the relevant analytical step UMAP of the hematopoiesis dataset colored by the 21 hematopoietic clusters UMAP was constructed using LSI estimation with 25,000 landmark cells Heatmap of 215,916 ATAC-seq marker peaks across all hematopoietic clusters identified with bias-matched differential testing Color indicates the column Z score of normalized accessibility Heatmap of motif hypergeometric enrichment-adjusted P values within the marker peaks of each hematopoietic cluster Color indicates the motif enrichment (−log10 (P value)) based on the hypergeometric test Side-by-side UMAPs of gene scores (left) and motif deviation scores for ArchR-identified TFs (right) for which the inferred gene expression is positively correlated with the chromVAR TF deviation across hematopoiesis representing positive TF regulators of hematopoiesis Lines are colored by the 21 clusters shown in c Genome accessibility track visualization of marker genes with peak co-accessibility 208,034,682–208,134,683) showing greater accessibility in earlier hematopoietic clusters (1–5 139,963,285–140,023,286) showing greater accessibility in earlier monocytic clusters (13–15) Schematic of scATAC-seq alignment with scRNA-seq data in m slices of n single cells These slices are independently aligned to a reference scRNA-seq dataset and then the results are combined for downstream analysis This integrative design facilitates rapid large-scale integration with low memory requirements UMAPs of scATAC-seq data from the hematopoiesis dataset colored by alignment to previously published hematopoietic scRNA-seq-derived clusters (b) integrated scRNA-seq gene expression for key marker TFs and genes (c) or cell alignment to the ArchR-defined B cell trajectory (d) the smoothed arrow represents a visualization of the interpreted trajectory (determined in the LSI subspace) in the UMAP embedding Heatmap of 11,999 peak-to-gene links identified across the B cell trajectory with ArchR Genome track visualization of the HMGA1 locus (chr6 34,179,577–34,249,577) (f) and the BLK locus (chr8 Single-cell gene expression (exp.) across pseudotime in the B cell trajectory is shown to the right Inferred peak-to-gene links for distal regulatory elements across the hematopoiesis dataset are shown below Heatmap of positive TF regulators for which gene expression is positively correlated with chromVAR TF deviation across the B cell trajectory Tn5 bias-adjusted TF footprints for nuclear factor early B cell factor (EBF)1 (j) and interferon regulatory factor (IRF)8 (k) motifs representing positive TF regulators across the B cell trajectory Lines are colored by the position in pseudotime of B cell differentiation In addition to the dramatic improvements in runtime ArchR supports state-of-the-art chromatin-based analyses including genome-wide inference of gene activity TF footprinting and data integration with matched scRNA-seq enabling statistical linkage of cis- and trans-acting regulatory factors to gene expression profiles the improvements from ArchR enable interactive data analysis by which end users can iteratively adjust analytical parameters and thus optimize identification of biologically meaningful results This is especially important in the context of single-cell data for which a one-size-fits-all analytical pipeline is not relevant or desirable resolution of subtle batch effects and biology-driven data exploration are intrinsically necessary for a successful scATAC-seq analysis and ArchR supports these efforts by enabling rapid analytical processes ArchR provides an open-source analysis platform with the flexibility speed and power to support the rapidly increasing efforts to understand complex tissues organisms and ecosystems at the resolution of individual cells All analyses were performed with the hg19 genome (except for the Mouse Atlas R-based analysis used the BSgenome package with ‘BSgenome.Hsapiens.UCSC.hg19’ (‘BSgenome.Mmusculus.UCSC.mm9’ for Mouse Atlas) for genomic coordinates and the TxDb package with ‘TxDb.Hsapiens.UCSC.hg19.knownGene’ (‘TxDb.Mmusculus.UCSC.mm9.knownGene’ for Mouse Atlas) gene annotations unless otherwise stated abbreviations are used for cell types of the hematopoietic system lymphoid-primed multipotent progenitor cell; B granulocyte macrophage progenitor; CD4 mem plasmacytoid dendritic cell; pre-B; pre-B cell; NK Bulk ATAC-seq libraries were pooled and purified by polyacrylamide gel electrophoresis before sequencing on an Illumina HiSeq 4000 using paired-end 75-bp reads and a union set of variable-width accessible regions was identified using bedtools merge (version 2.26.0) These accessible regions were genotyped across all samples using SAMtools mpileup (version 1.5) and VarScan mpileup2snp (version 2.4.3) with the following parameters: ‘--min-coverage 5 --min-reads2 2 --min-var-freq 0.1 --strand-filter 1 --output-vcf 1’ All positions containing a single-nucleotide variant were compiled into a master set and then each cell line was genotyped at those specific single-base locations using SAMtools mpileup The allelic depth at each position was converted into a quaternary genotype (homozygous A homozygous B or insufficient data to generate a confident call) inferred genotype probabilities were created based on those quaternary genotypes and a VCF file was created for input to demuxlet using recommended parameters Demuxlet was used to identify the cell line of origin for individual cells and to identify doublets based on mixed genotypes Further information on research design is available in the Nature Research Reporting Summary linked to this article A Correction to this paper has been published: https://doi.org/10.1038/s41588-021-00850-x Single-cell chromatin accessibility reveals principles of regulatory variation Multiplex single cell profiling of chromatin accessibility by combinatorial cellular indexing The cis-regulatory dynamics of embryonic development at single-cell resolution Integrated single-cell analysis maps the continuous regulatory landscape of human hematopoietic differentiation A single-cell atlas of in vivo mammalian chromatin accessibility Massively parallel single-cell chromatin landscapes of human immune cell development and intratumoral T cell exhaustion Single-cell multiomic analysis identifies regulatory programs in mixed-phenotype acute leukemia Droplet-based combinatorial indexing for massive-scale single-cell chromatin accessibility Pierce, S. E., Granja, J. M. & Greenleaf, W. J. High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer. Preprint at bioRxiv https://doi.org/10.1101/2020.11.02.364265 (2020) Assessment of computational methods for the analysis of single-cell ATAC-seq data Fang, R. et al. Fast and accurate clustering of single cell epigenomes reveals cis-regulatory elements in rare cell types. Preprint at bioRxiv https://doi.org/10.1101/615179 (2019) Comprehensive integration of single-cell data BROCKMAN: deciphering variance in epigenomic regulators by k-mer factorization Single-cell regulome data analysis by SCRAT chromVAR: inferring transcription-factor-associated accessibility from single-cell epigenomic data cisTopic: cis-regulatory topic modeling on single-cell ATAC-seq data Cicero predicts cis-regulatory DNA interactions from single-cell chromatin accessibility data Unsupervised clustering and epigenetic classification of single cells The Encyclopedia of DNA elements (ENCODE): data portal update DoubletFinder: doublet detection in single-cell RNA sequencing data using artificial nearest neighbors Scrublet: computational identification of cell doublets in single-cell transcriptomic data Multiplexed droplet single-cell RNA-sequencing using natural genetic variation The accessible chromatin landscape of the human genome Determinants of enhancer and promoter activities of regulatory elements Analysis and function of transcriptional regulatory elements: insights from Drosophila Single-cell RNA-seq reveals AML hierarchies relevant to disease progression and immunity Augmented implicitly restarted Lanczos bidiagonalization methods destiny: diffusion maps for large-scale single-cell data in R Geometric diffusions as a tool for harmonic analysis and structure definition of data: diffusion maps Lineage-specific and single-cell chromatin accessibility charts human hematopoiesis and leukemia evolution McInnes, L., Healy, J. & Melville, J. UMAP: Uniform Manifold Approximation and Projection for dimension reduction. Preprint at https://arxiv.org/abs/1802.03426 (2018) The chromatin accessibility landscape of primary human cancers Landscape of stimulation-responsive chromatin across diverse human immune cells Corces, M. R. et al. Single-cell epigenomic identification of inherited risk loci in Alzheimer’s and Parkinson’s disease. Preprint at bioRxiv https://doi.org/10.1101/2020.01.06.896159 (2020) Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements Transcript-indexed ATAC-seq for precision immune profiling An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues Slingshot: cell lineage and pseudotime inference for single-cell transcriptomics The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells Reversed graph embedding resolves complex single-cell trajectories The single-cell transcriptional landscape of mammalian organogenesis SCANPY: large-scale single-cell gene expression data analysis Download references We thank members of the Greenleaf and Chang laboratories for helpful comments This work was supported by the NIH RM1-HG007735 and UM1-HG009442 (to H.Y.C K99-AG059918 and the American Society of Hematology Scholar Award (to M.R.C.) an International Collaborative Award (to H.Y.C the Defense Advanced Research Project Agency (W911NF1920185 to W.J.G.) a gift from the Ray and Dagmar Dolby Family Fund (to the Gladstone Institutes) and a Stanford Cancer Institute-Goldman Sachs Foundation Cancer Research Award (to W.J.G) is an Investigator of the Howard Hughes Medical Institute These authors contributed equally: Jeffrey M Gladstone Institute of Neurological Disease Gladstone Institute of Data Science and Biotechnology led the design of the ArchR software with input from S.E.P led the scATAC-seq data creation with input from S.T.B. led the single-cell analysis presented in this paper wrote the manuscript with input from all authors which has licensed IP associated with ATAC-seq has additional affiliations with Guardant Health (consultant) and Protillion Biosciences (cofounder and consultant) is a cofounder of Accent Therapeutics and Boundless Bio and is a consultant for Arsenal Biosciences and Spring Discovery Peer review information Nature Genetics thanks the anonymous reviewers for their contribution to the peer review of this work Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Comparison of comprehensiveness of supported scATAC-seq features across ArchR and other existing software packages (d-e) ~10,000 cells from the PBMCs dataset using (d) 32 GB of RAM and 8 cores or (e) 128 GB of RAM and 20 cores (f-g) ~30,000 cells from the PBMCs dataset using (f) 32 GB of RAM and 8 cores or (g) 128 GB of RAM and 20 cores and (h-i) ~30,000 cells from the bone marrow dataset using (h) 32 GB of RAM and 8 cores or (i) 128 GB of RAM and 20 cores Dots represent individual replicates of benchmarking analysis (N = 3) Benchmarks from ArchR for run time and peak memory usage for the analysis of ~70,000 cells from the sci-ATAC-seq mouse atlas dataset (N = 13 tissues) for (left) 32 GB of RAM with 8 cores and (right) 128 GB of RAM with 20 cores Dots represent individual replicates of benchmarking analysis t-SNE of mouse atlas scATAC-seq data (N = 64,286 cells) colored by individual samples QC filtering plots from ArchR for (top) replicate 1 and (bottom) replicate 2 from the cell line mixing dataset showing the TSS enrichment score vs unique nuclear fragments per cell Dot color represents the density in arbitrary units of points in the plot Accuracy of various doublet prediction methods for (top) replicate 1 and (bottom) replicate 2 from the cell line mixing dataset measured by the area under the curve (AUC) of the receiver operating characteristic (ROC) Accuracy is determined with respect to genotype-based identification of doublets using demuxlet ‘KNN’ represents the number of cells nearby each projected synthetic doublet to record when calculating doublet enrichment scores The distance for KNN recording is determined in the LSI subspace for LSI projection and in the UMAP embedding for UMAP projection parameters The smooth line represents a LOESS fit (shading represents 95% confidence interval) UMAP of scATAC-seq data showing the (c-d) simulated doublet density or (g-h) cell line identity based on genotyping information and demuxlet for (c,e,g) replicate 1 (N = 15,345 cells) and (d,f,h) replicate 2 (N = 22,727 cells) of the cell line mixing dataset Precision recall (PR) curves of doublet prediction using (left) ArchR and (right) Scrublet doublet identification or the number of nuclear fragments per cell compared to demuxlet as a ground truth UMAP of PBMC mixing scRNA-seq from Kang et Cells are colored as doublets (black) or singlets (gray) as identified by (left) demuxlet or (right) ArchR (f) ROC curves or (g) PR curves of doublet prediction using ArchR (dark blue) or the number of total counts per cell (light blue) compared to demuxlet as a ground truth Schematic of 10x Genomics Multiome workflow (left) TSS enrichment score vs unique nuclear fragments per cell (color is density) or (right) aggregate fragment size distributions for the cells passing ArchR QC thresholds from the PBMC Multiome data Distribution of (top) the number of unique molecular identifiers (nUMIs) per cell passing scATAC-seq filtration and (bottom) the number of unique genes (nGenes) identified with at least 1 UMI per cell UMAPs of (left) scATAC-seq data or (right) scRNA-seq data from the Multiome dataset shown (top) with doublets present (black N = 10,887) and (bottom) with ArchR-identified doublets removed (N = 9,702) (l) ROC and (m) PR curves of doublet prediction using ArchR (red) compared to the top doublets (N = 750) identified by Scrublet as a ground truth and vice versa (blue) UMAP of scATAC-seq data from CD34 + bone marrow cells (green orange) and unfractionated bone marrow cells (blue and red) colored by (left) sample and (right) ArchR identified clusters (N ~ 30,000 cells total) Plots are shown (left pair) without doublet removal and (right pair) with ArchR-based doublet removal Schematic of the iterative LSI procedure implemented in ArchR for dimensionality reduction Schematic of the downsampling approach used on bulk ATAC-seq data to enable evaluation of clustering performance for simulated scATAC-seq data Bar plot showing the number of replicates generated per cell type by downsampling of bulk ATAC-seq data from hematopoietic cells t-distributed stochastic neighbor embedding (t-SNE) of downsampled bulk ATAC-seq data from hematopoeitc cells (N = 7,200) to various data quality scales Left; low-quality scATAC-seq data (~1,000 fragments/cell) Middle; medium-quality scATAC-seq data (~5,000 fragments/cell) Right; high-quality scATAC-seq data (~10,000 fragments/cell) t-SNE plots were created for (top) ArchR (iterative LSI) these t-SNE plots are colored by (top) cell type and (bottom) sample replicate Adjusted Rand Index (ARI) of clusters identified by ArchR (blue) medium-quality and high-quality downsampling of bulk ATAC-seq data from hematopoietic cells Distribution of Pearson correlations of inferred gene score and aligned gene expression for (a,c,e,g) each gene or (b,d,f,h) each cell group across groups of 100 cells (N = 500 groups) Distributions are either presented for (a,b,e,f) the top 1,000 differentially expressed genes or (c,d,g,h) the top 2,000 most variable genes for each of the 56 gene score models The red dotted line represents the median value of the best-performing model Violin plots represent the smoothed density of the distribution of the data the lower whisker is the lowest value greater than the 25% quantile minus 1.5 times the interquartile range the upper hinge is the 75% quantile and the upper whisker is the largest value less than the 75% quantile plus 1.5 times the interquartile range UMAPs of scATAC-seq data from (i) cells from the PBMCs dataset (N = 27,845 cells) or (j) cells from the bone marrow cell dataset (N = 26,748 cells) colored by (top) inferred gene scores or (bottom) gene expression for several marker genes Schematic illustrating the methodology used to assess the accuracy of inferred gene scores Heatmaps summarizing the accuracy (Pearson correlation) across all models for both the top 1,000 differentially expressed and top 2,000 variable genes for bulk ATAC-seq and RNA-seq from hematopoietic cell types Each entry is colored by the model rank in the given test as described below the heatmap Heatmaps of (left) gene expression or (right) gene scores for the top 1,000 differentially expressed genes (selected from bulk RNA-seq) across all cell types from the matched bulk ATAC-seq and RNA-seq data Heatmaps summarizing the accuracy (measured by Pearson correlation) across all models for both the top 1,000 differentially expressed and top 2,000 variable genes for paired scATAC-seq and scRNA-seq data The model class is indicated to the left of each heatmap UMAPs of scATAC-seq data from the Multiome PBMCs dataset (N = 9,702 cells) colored by (bottom) inferred gene scores or (top) gene expression for several marker genes Heatmaps of (left) gene expression or (right) gene scores for the top 1,000 differentially expressed genes (selected from scRNA-seq) across all cell types from the paired scATAC-seq and scRNA-seq data Benchmarking of run time for TF footprinting with ArchR for the 102 sample-aware pseudo-bulk replicates from the hematopoiesis dataset a, Side-by-side UMAPs for the hematopoiesis dataset cells colored by (top) gene expression (log2(Normalized Counts +1)) from scRNA-seq alignment or (bottom) inferred gene scores (log2(Gene Score +1)) from gene score Model 42 (see Fig. 2c) for key immune marker genes Slingshot and Monocle3 scaled trajectory positions (Scaled TP) across the (left) B cell trajectory and (right) Myleoid trajectory Download citation DOI: https://doi.org/10.1038/s41588-021-00790-6 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing newsletter — what matters in science 2022 (GLOBE 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237-0331 or visit www.lagranjarestaurants.com This content was issued through the press release distribution service at Newswire.com "Nordic Aquafarms is disappointed in the May 7 decision by the Belfast City Council we remain optimistic while we continue to evaluate options that are in the interests of the company as well as the local community and the broader region," the company said in a statement Editor Magazine & Web: Gareth Moore Chief Editor: Pål Mugaas Jensen Publisher Oceanspace Media AS:Gustav Erik Blaalid Metrics details Despite widespread epigenetic heterogeneity within the patient cohort we observe common malignant signatures across patients as well as patient-specific regulatory features that are shared across phenotypic compartments of individual patients Integrative analysis of transcriptomic and chromatin-accessibility maps identified 91,601 putative peak-to-gene linkages and transcription factors that regulate leukemia-specific genes such as RUNX1-linked regulatory elements proximal to the marker gene CD69 multiomic analysis of single cells within the framework of normal development can reveal both distinct and shared molecular mechanisms of disease from patient samples Prices may be subject to local taxes which are calculated during checkout Sequencing data are deposited in the Gene Expression Omnibus (GEO) with the accession code GSE139369 There are no restrictions on data availability or use Code used in this study can be found on Github at https://github.com/GreenleafLab/MPAL-Single-Cell-2019 Cell-of-origin patterns dominate the molecular classification of 10,000 tumors from 33 types of cancer Cell-of-origin chromatin organization shapes the mutational landscape of cancer Mixed-phenotype acute leukemia: historical overview and a new definition The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia Simultaneous epitope and transcriptome measurement in single cells Massively parallel digital transcriptional profiling of single cells Integrating single-cell transcriptomic data across different conditions McInnes, L., Healy, J. & Melville, J. UMAP: Uniform manifold approximation and projection for dimension reduction. Preprint at arXiv https://arxiv.org/abs/1802.03426 (2018) The genetic basis and cell of origin of mixed phenotype acute leukaemia Integrative genomic analysis of adult mixed phenotype acute leukemia delineates lineage associated molecular subtypes High-throughput chromatin accessibility profiling at single-cell resolution Li, B. et al. Census of immune cells. HCA https://data.humancellatlas.org/explore/projects/cc95ff89-2e68-4a08-a234-480eca21ce79 (2018) IL1RAP potentiates multiple oncogenic signaling pathways in AML clusterProfiler: an R package for comparing biological themes among gene clusters cyclins and CKIs: roles beyond cell cycle regulation How I treat mixed-phenotype acute leukemia What is the optimal treatment for biphenotypic acute leukemia Biallelic and heterozygous point mutations in the runt domain of the AML1/PEBP2αB gene associated with myeloblastic leukemias Landscape of genetic lesions in 944 patients with myelodysplastic syndromes The genetic basis of early T-cell precursor acute lymphoblastic leukaemia Reverse engineering of TLX oncogenic transcriptional networks identifies RUNX1 as tumor suppressor in T-ALL Breast tumors educate the proteome of stromal tissue in an individualized but coordinated manner Core transcriptional regulatory circuit controlled by the TAL1 complex in human T cell acute lymphoblastic leukemia Addiction of t(8;21) and inv(16) acute myeloid leukemia to native RUNX1 RUNX1 is a key target in t(4;11) leukemias that contributes to gene activation through an AF4–MLL complex interaction Single-cell multi-omic integration compares and contrasts features of brain cell identity CD69 association with Jak3/Stat5 proteins regulates Th17 cell differentiation CD69 acts downstream of interferon-α/β to inhibit S1P1 and lymphocyte egress from lymphoid organs The role of the Runx transcription factors in thymocyte differentiation and in homeostasis of naive T cells New insights on the transcriptional regulation of CD69 gene through a potent enhancer located in the conserved non-coding sequence 2 Discovery of stimulation-responsive immune enhancers with CRISPR activation Combined cistrome and transcriptome analysis of SKI in AML cells identifies SKI as a co-repressor for RUNX1 Cancer Genome Atlas Research Network Genomic and epigenomic landscapes of adult de novo acute myeloid leukemia edgeR: a Bioconductor package for differential expression analysis of digital gene expression data robustness and scalability in single-cell differential expression analysis Download references Satpathy and other members of the Chang and Greenleaf laboratories for helpful discussions We thank the following people at 10x Genomics: D Ziraldo for help with sample preparation and library generation of scATAC-seq and feature barcoding libraries We acknowledge the Stanford Hematology Division Tissue Bank for providing samples for this study This study was supported by the Swedish Research Council (grant 2015–06403 is supported by grant K99AG059918 (NIA) and the American Society of Hematology Scholars award Further support came from National Institutes of Health grants P50-HG007735 and UM1-HG009442 (to H.Y.C as well as from Ludwig Cancer Research (to R.M and H.Y.C.) and grants from the Chan-Zuckerberg Initiative and the Rita Allen Foundation was supported by The Stanford Genome Training Program (NIH/NHGRI) was supported by the JIMB/NIST training program Department of Medical Biochemistry and Biophysics conceived the project and designed the experiments led data production and performed the experiments together with A.S.K. provided healthy bone marrow and peripheral blood CITE-seq data analyzed the scADT-seq data with contribution from B.P conceived the analytical workflows and performed the data analysis for scATAC-seq and scRNA-seq supervised by H.Y.C and W.J.G wrote the manuscript with input from all authors and serves on the board of directors of Forty Seven has affiliations with Accent Therapeutics (founder and scientific advisory board (SAB) member) Arsenal Biosciences (SAB) and Spring Discovery (SAB member) has affiliations with 10x Genomics (consultant) Guardant Health (consultant) and Protillion Biosciences (co-founder and consultant) (a) Number of cells passing filter for each experimental replicate (number of informative genes > 400 and number of unique molecular identifiers (UMI) > 1000) (b) Number of aligned reads on average per cell passing filter for each experimental sample (c) Violin and box-whisker plot of the number of informative genes detected per single cell passing filter per experimental sample (n = 2,424 – 7,544) (d) Violin and box-whisker plot of the number of unique molecular identified (UMI) transcripts per cell passing filter per experimental sample (n = 2,424 – 7,544) (e) Aggregated scRNA-seq (n = 20,287) one to one reproducibility plots for biological replicates (left) and across sample types (right) colored by the density The correlation (r) represents the Pearson correlation across all genes (f) scRNA-seq experimental sample labels overlay on UMAP of hematopoiesis (n = 35,582) (g) scADT-seq UMAP of BMMC and PBMC samples (n = 4) across 14 antibodies scADT overlay of experimental sample labels Color represents experimental labels or scADT-seq values after CLR transformation (h) scADT-seq UMAP of BMMC and PBMC samples (n = 4) across 14 antibodies colored by scRNA-seq clusters with biological classification Box-whisker plot; lower whisker is the lowest value greater than the 25% quantile minus 1.5 times the interquartile range (IQR) the upper hinge is the 75% quantile and the upper whisker is the largest value less than the 75% quantile plus 1.5 times the IQR (a) scATAC-seq cell filtering plot of 4 representative scATAC-seq hematopoietic samples The x-axis is the number of unique accessible fragments and the y-axis is the enrichment of Tn5 insertions at transcription start sites representing the robust signal to background for each single cell (b) Aggregated scATAC-seq fragment size distributions across individual experiments demonstrating sub- mono- and multi nucleosome spanning ATAC-seq fragments (c) Number of cells passing filter for each experimental replicate (Unique nuclear fragments > 1000 and TSS enrichment > 8) (d) Violin and box-whisker plot of the number of total aligned fragments for each single cell passing filter per experimental sample (n = 836 – 12,394) (e) Violin and box-whisker plot of the number of unique aligned nuclear fragments for each single cell passing filter per experimental sample (n = 836 – 12,394) (f) Violin and box-whisker plot of the fraction of the total number Tn5 insertions (Reads) that are within the healthy hematopoietic union peak set (n = 452,004) for each single cell passing filter (g) Violin and box-whisker plot of the normalized transcription start site (TSS) enrichment for each single cell passing filter per experimental sample (h) Aggregated scATAC-seq (n = 452,004) one to one reproducibility plots for biological replicates colored by the density (i) scATAC-seq experimental sample labels overlay on UMAP of hematopoiesis (n = 35,038) (a-h) Multi-omic tracks; (Top) average track of all clusters displayed (Middle) binarized 100 random scATAC-seq tracks for each locus at 100bp resolution and (right) violin and box-whisker plot of the scRNA-seq log2 normalized expression for each cluster (a) Multi-omic track of GATA1 (specific in these clusters for Erythroid) for erythroid development from HSC progenitor cells (n = 111 - 1,653) (b) Multi-omic track of GATA2 (specific in these clusters for Basophil) for erythroid development from HSC progenitor cells (n = 111 - 1,653) (c) Multi-omic track of ELANE (specific in these clusters for GMP/Neutrophil) for neutrophil development from HSC progenitor cells (n = 1,050 – 2,260) (d) Multi-omic track of IRF8 (specific in these clusters for pDC) across pDC development from HSC progenitor cells (n = 544 – 2,260) (e) Multi-omic track of SDC1 (specific in these clusters for Plasma cells) across B cell development and plasma cells (n = 62 – 2,260) (f) Multi-omic track of CD1C (specific in these clusters for cDC) across cDC development from HSC progenitor cells (n = 325 – 2,260) (g) Multi-omic track of SELL (specific in these clusters for Naive T cells vs memory and CD8 central memory vs CD8 effector memory) across NK and T cells (n = 796 – 3,539) (h) Multi-omic track of GZMB (specific in these clusters for NK cells) across NK and T cells (n = 796 – 3,539) (a-f) Diagnostic flow cytometry plots from the 5 different MPAL cases (MPAL1-5R) gated on blasts area (highlighted in red) and lymphocytes (highlighted in black) from CD45 and side scatter area (SSC-A) (a) MPAL 1 shows classic bilineal phenotype with both T-lymphoblasts (cCD3-positive and CD7-positve) and myeloid blasts (MPO-positive and CD33-positive) (b) MPAL 2 demonstrates a more complex phenotype with both biphenotypic (single population expressing lymphoid marker CD7 and myeloid marker CD33) and bilineal T-Myeloid patterns (subpopulation expressing monocytic markers CD64 (c) MPAL 3 demonstrates a classic biphenotypic case with coexpression of both T-lineage markers (cCD3-positive) and myeloid markers (MPO-positive) (d) MPAL4 demonstrates a classic bilineal B/M phenotype expressing B-lineage markers (CD79a and CD19-positive) and myeloid markers (MPO-positive and CD33-positive) (e) MPAL5 demonstrates a more complicated phenotype with a subpopulation of blasts expressing T-lineage markers (cCD3-positive and CD7-positive) and a subpopulation expressing myeloid marker MPO (f) MPAL5R post-treatment relapse of MPAL5 Flow cytometry reveals expansion of the T-lymphoblastic subpopulation (cCD3-positive TdT-positive population) following chemotherapy (g) High-confidence mutations detected in 5 MPAL cases by whole exome sequencing and nonframeshift deletions are shown in dark gray (c) Violin and box-whisker plot of the number of informative genes detected per single cell passing filter per experimental sample (d) Violin and box-whisker plot of the number of unique molecular identified (UMI) transcripts per cell passing filter per experimental sample (e) Aggregated scRNA-seq (n = 20,287) one to one reproducibility plots for technical replicates colored by the density (f) scATAC-seq cell filtering plot of 6 representative scATAC-seq MPAL samples (g) Aggregated scATAC-seq fragment size distributions across individual experiments demonstrating sub- (h) Number of cells passing filter for each experimental replicate (Unique nuclear fragments > 1000 and TSS enrichment > 8) (i) Violin and box-whisker plot of the number of total aligned fragments for each single cell passing filter per experimental sample (j) Violin and box-whisker plot of the number of unique aligned nuclear fragments for each single cell passing filter per experimental sample (k) Violin and box-whisker plot of the fraction of the total number Tn5 insertions (Reads) that are within the MPAL union peak set (n = 346,274) for each single cell passing filter (l) Violin and box-whisker plot of the normalized transcription start site (TSS) enrichment for each single cell passing filter per experimental sample (m) Aggregated scATAC-seq (n = 346,274) one to one reproducibility plots for technical replicates colored by the density (Left) scRNA-seq projections of previous study healthy bone marrow cells (different platform and different aligned genome) colored by previous classifications (Right) scATAC-seq projections for healthy bone marrow and peripheral blood samples (2 different platforms across 3 studies) colored by ground truth isolated populations (d) Projection of hematopoietic scRNA-seq into of Human Cell Atlas (HCA) Census of Immune Cells (Left) Number of cells per each of 8 bone marrow donors (Middle) UMAP projection of LSI iterative clustering of HCA bone marrow scRNA-seq (Right) LSI projection of our scRNA-seq hematopoietic single cells into HCA bone marrow UMAP colored by cluster definitions (a) MPAL single cell classification workflow for scRNA and scATAC-seq First cells were clustered with reference hematopoietic cells (1) and classified based on healthy hematopoietic clusters (2-3) Cells were then LSI projected into the reference hematopoietic manifold (4) and then classified based on the nearest reference cell hematopoietic compartment (5) MPAL 5 replicate 1 is shown as an example for scRNA (Top) and scATAC-seq (Bottom) (b) Proportion of estimated blast cells for each MPAL with Flow Cytometry scRNA and scATAC-seq (Range is from 0 to 1) (c) (Left) Projected MPALs colored by hematopoietic compartments as described in a CD4 and CD19 on MPAL single cells LSI projected onto hematopoiesis (a) Top conserved differential genes across the MPAL hematopoietic compartments (b) Top conserved differential transcription factors across the MPAL hematopoietic compartments (c) KEGG pathway enrichment of genes (scRNA) differentially conserved k-means 2 Color represents the significance (hypergeometric test adjusted p-value with the Benjamini-Hochberg correction) of the KEGG path way generated using clusterProfiler (Yu (d-e) Multi-omic differential tracks (Left) scATAC tracks showing MPAL disease subpopulations (red) closest normal cells (gray) (Right) Violin plot of the log2 normalized expression for MPAL disease subpopulations (red) and closest normal cells (gray); black line represents the mean and asterisk denote significance (LFC > 0.5 and FDR < 0.01 from Figure 2c) (d) Multi-omic differential track of CDK11A (e) Multi-omic differential track of CDKN2A (d) Binary heatmap of variable malignant genes across leukemia classifications Each cell in the heatmap is colored whether the gene was identified as malignant for the leukemic sample (a) UMAP of CCA alignment of scATAC-seq using Cicero gene activity scores and scRNA-seq for (Left) bone marrow (nATAC = 12,602; nRNA = 16,510) (Middle) CD34+ enriched bone marrow (nATAC = 8,176; nRNA = 10,160) (Right) peripheral blood (nATAC = 14,804; nRNA = 8,368) (b) UMAP of CCA alignment of scATAC-seq using Cicero gene activity scores and scRNA-seq for MPAL samples 1-5R (nATAC = 4,127 – 8,255; nRNA = 835 – 5,885) (a) Spearman rank correlation between scATAC-seq Cicero gene activity scores to scRNA-seq for each mapped cell within across all biological experiments (n = 4,127 – 16,510) (b) Pearson correlation of CCA scRNA and scATAC-seq nearest-neighbors The cutoff (R > 0.45) for high quality nearest neighbor mappings is shown (n = 4,127 – 16,510) (c) (Left) UMAP of scATAC-seq hematopoiesis colored by scATAC-seq clusters (n = 35,038) (Right) UMAP of scATAC-seq hematopoiesis colored by filtered mapped scRNA-seq clusters (n = 34,507) (d) Confusion matrix of initial clusters for mapped scRNA-seq to scATAC-seq clusters for hematopoiesis (Figure 1b-c) Above shows the assigned biological classifications for each cluster across both scRNA and scATAC-seq (e) (Left) Distribution of peak-to-gene distances (Left-Middle) Distribution of number of peaks mapped per gene (median = 6) (Right-Middle) Distribution of number of genes mapped per peak (median = 1) (Right) Distribution of number of genes skipped for peak-to-gene links (median = 2) (f) MetaV4C plots of K27ac HiChIP in Naive T and HCASMC cells for top 500 biased T/NK (broad classification) peak-to-gene links that are identified only in healthy hematopoiesis The line represents the average signal and the shading represents the range of the signal times the square root of 2 between biological replicates (n = 2) (g) Peak-to-gene links (n = 91,601) enrichment in GTEx eQTLs over a permuted background distance-matched set (permutations = 250) for the union set of peak-to-gene links and the error bars indicate 1 standard deviation (a-d) Multi-omic differential track; (Middle) Aggregated scATAC tracks showing MPAL disease subpopulations (red) and closest normal cells (gray) (Right) Distribution of log2 normalized expression of gene of interest for MPAL disease subpopulations (red) and closest normal cells (gray); black line represents the mean and asterisk denote significance (LFC > 0.5 and FDR < 0.01 from Figure 2c) Violin plot represents the smoothed density of the distribution of the log2 normalized expression and the black line represents the mean log2 normalized expression (Bottom) Peak-to-gene links for gene of interest colored by Pearson correlation of the peak accessibility and gene expression (see methods) (a) Multi-omic differential track for IL1RAP (n = 89 - 500) (b) Multi-omic differential track for CD96 (n = 89 - 500) (c) Multi-omic differential track for FLT3 (n = 89 - 500) (d) Multi-omic differential track for MCL1 (n = 89 - 500) Briefly cells are aligned using 10x cell ranger and clustered using a feature optimization approach (see methods) Briefly cells are aligned using 10x cell ranger atac creation of a counts matrix and clustered using a feature optimization approach (see methods) Healthy donor information: sex and age range Information from patients with MPAL: WHO diagnosis Hematopoiesis cluster biological classification labels are also included Antibodies used in flow cytometry of MPALs Antibody information for hematopoietic and MPAL samples as well as barcodes used for sequencing ADT libraries Differential genes and peaks for MPAL differential RNA-seq k-means AML differential RNA-seq k-means and an MPAL versus AML comparison Motif enrichment and linkage to target genes. MPAL differential ATAC-seq k-means enrichment for CIS-BP motifs shown in Fig. 3a significant peak-to-gene links and RUNX1-target genes are shown Download citation DOI: https://doi.org/10.1038/s41587-019-0332-7 Journal of Analytical Science and Technology (2025) Sign up for the Nature Briefing: Cancer newsletter — what matters in cancer research Below is a list of places that were ordered shut last week by the Florida Department of Business and Professional Regulation Talay Thai has not been re-inspected and has not been allowed to re-open All the others mentioned have been allowed to re-open following an ordered cleanup and re-inspection ALSO ORDERED SHUT 3/5/21(ROACH INFESTATION) “Rodent activity present as evidenced by rodent droppings found Approximately 40 Rodent droppings on storage area on the floor and top shelf above reach in freezer Approximately 15 rodent droppings on spice shelf on kitchen area Approximately 30 rodents droppings under both microwaves on kitchen area Approximately 3 rodent droppings on top of bread crumb plastic container Approximately 20 rodent droppings on top shelf dishwasher area Approximately 6 rodent droppings on front counter shelf Advised operator to remove Observed 2 dead roaches inside small fridge under sushi bar “Accumulation of dead small flying insects under microwave on sushi bar area “Raw animal food stored over/not properly separated from ready-to-eat food Raw shrimp over wontons inside walk-in cooler Raw chicken over wontons inside reach in freezer “Time/temperature control for safety food cold held at greater than 41 degrees Fahrenheit Observed seating on cook line Raw shell eggs 68°F cold Holding Observed inside walk-in cooler Wontons 49°F cold Holding Per operator products stored for approximately 2 hours Per operator products not prepared or portioned today Operator moved eggs and bean sprouts to inside of reach in cooler and moved items from walk-in cooler to reach in freezer hot held at less than 135 degrees Fahrenheit Observed seating on stovetop with no flame a container with curry sauce 110°F hot Holding Per operator product stored for approximately 2 hours Operator turned on flame to reheat product.” “Outdoor ice machine with no overhead protection Overhead does not cover front part of ice machine,” “Roach activity present as evidenced by live roaches found Observed Approximately 15 live roaches in the kitchen underneath Non-working reach in cooler next to Prep sink.” Observed 1 dead roach next to ice equipment 1 underneath Dishwashing equipment floor 1 in front of walk-in cooler floor “Accumulation of debris on exterior of ware washing machine “Accumulation of lime scale on the inside of the dish machine.” “Certified Food Manager or person in charge lacks knowledge of food borne illnesses and symptoms of illness that would prevent an employee from working with food Observed *>20 live flies landed on wall by service/prep side station *>20 live flies flying around and landing on boxes and single service pizza boxes and storage racks in dry storage area next to service station plates and utensils in service/prep side station *4 flying around and landing on boxes and wall by rear door *3 live flies on wall under prep table next to room with hot water heater *3 live flies flying around in prep area next to hot water heater room *4 live flies flying around in dishwashing area and landing on sanitized pans *2 live flies flying around pantry/salad station by cook line *3 live flies flying around in pizza station *5 live flies flying around and landing on tables and seats in dining room.” and drink ice uncovered with live flies flying around in service/prep area Observed *>100 dead flies on two insect strips hanging from ceiling in dry storage area above single service pizza boxes *>10 dead flies on strip hanging from ceiling in dry storage room.” “Employee touching ready-to-eat food with their bare hands - food was not being heated as a sole ingredient to 145 degrees F or immediately added to other ingredients to be cooked/heated to the minimum required temperature to allow bare hand contact Establishment has no approved Alternative Operating Procedure -Observed server placed berries in customer drink with bare hands.” Operator said in unit approximately 1 hour “Hand wash sink not accessible for employee use at all times -Observed garbage can stored in front of hand wash sink in prep area next to hot water heater room - Observed cut lemons in hand wash sink by service/prep station Operator removed garbage can and remove lemons.” -Observed water leaking under hand wash sink in dishwashing area.” “Standing water or very slow draining water in hand wash sink -Observed water slow draining at hand wash sink next to walk-in cooler/freezer.” -Observed wall soiled with dust towards rear door -Observed ceiling soiled with dust in dishwashing area.” -Observed floor under hand wash sink in dishwashing area soiled with mold like substance.” “Required employee training expired for some employees.” Copyright 2022 by WPLG Local10.com - 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When you check your social media notifications or enter your destination into a rideshare app, you’re interacting with a screen — but also, invisibly, with user experience (UX) designers.  These tech professionals mapped out your experience long before you had it structuring it to feel as natural as possible That’s their job: to craft easily navigable digital interfaces Doing so takes empathy and artistry But how are those qualities applied on a day-to-day basis We asked a couple of UX designers to give us a peek into their worlds Both agreed that UX design roles break down into roughly four components: Dobe says she spends up to 75 percent of her work hours on ideating Granja focuses mostly on designing and communicating UX design roles vary widely, and there are countless possible UX design specializations like information architecture and communication design That’s not to mention another possible path in the field: generalist Especially on smaller UX design teams like Granja’s every designer does a little of everything Both Dobe and Granja describe the UX design workflow as cyclical. On a given feature, user research spawns new front-end designs UX designers often find themselves in multiple workflows simultaneously Dobe has worked on as many as nine projects at once a more detailed look at the four parts of UX design.  Dobe is now a full-time UX researcher but she did some research as a UX designer too — especially at the beginning of projects was always “to really understand a user’s needs motivations [and] empathize with them in terms of enhancing a product or even creating a new product.” Specifically, Dobe’s research duties ranged from reviewing quantitative and qualitative survey results to user testing Also known as usability testing user testing involves learning about the user experience by simply observing how users go about completing key tasks within an app or platform The process can highlight points of confusion in a more granular way than an interview might.  UX designers “really get to know [users],” Granja says of the process Her user testing focuses on the features that stakeholders agree need feedback Granja begins by laying out goals for testing identifying the key interfaces to study — for instance a homepage or a profile page — and the key learning objectives for each interface This is the standardized monologue UX designers read before a user test begins which lays out ground rules and introduces the task it might be uploading a photo; on LinkedIn it might be adding a new job to your resume as a “dress rehearsal” for a user test with consumers Granja asks her coworkers to try out a new feature.  User research can also involve user interviews would never ask a user something as straightforward as “What do you like about this product?” Instead she and Granja often ask users about their experiences in the industry completely separate from the products they’re researching.  “It’s the antithesis of asking people what they want,” Dobe says she and Granja might ask how a user currently gets their groceries as well as what goals and constraints influence the shopping process but blocking out time for ideating is central to the UX designer’s process — and the creative process in general This is how research findings become design concepts Ideating consists of two phases. The first: research analysis, during which designers home in on concrete user problems. This can involve straightforward data science Dobe breaks a sample of users down into categories and storyboards out the typical user experience for each type she notes common “pain points” for each type — for instance a final “purchase” button one type always misses usually synthesizes her research findings in a findings matrix: a table of problems and ratings of their difficulty levels and importance Difficulty depends on the developer and designer horsepower a task requires; importance depends on how much the problem interferes with basic user tasks Once a UX designer has picked a problem to tackle it’s time for the second part of ideating: brainstorming This doesn’t exactly involve pulling ideas “out of thin air,” Granja says. UX designers draw inspiration from wireframes for their brand’s other features to ensure the uniformity of user encounters They also incorporate trends from the web at large “You don’t want to make someone come in and relearn how to check out.” When Granja feels stumped, she browses Dribbble and Behance for inspiration But brainstorming doesn’t have to be a solo process Both she and Dobe like to brainstorm with colleagues talking through problems and jotting ideas on a whiteboard inviting them to brainstorming (or “jam”) sessions at which they collaboratively sketched out possible designs and voted on their favorites The ideating phase ends when a UX designer settles on a particular concept they want to move forward with During this phase, a UX designer transforms a concept into a rough draft. How rough depends on the level of fidelity, or faithfulness, with which they’ve been tasked. A low-fidelity design, or wireframe, is a much rougher draft than a high-fidelity design, or prototype The former is more like a blueprint or outline of “the structure of the site,” Dobe says The latter has some interactive features and fleshed-out visuals and is pretty close to a finished product.  UX designers typically focus on wireframes. When Dobe was a designer, she created hers using software like Axure and Sketch wireframing is a process of positioning logos menus and buttons on highly-trafficked pages Actual imagery remains minimal in this phase It’s more about indicating placement for later design phases and creating a layout that draws attention to the most essential information Granja also wireframes in Sketch, which she describes as a streamlined Photoshop. Because her job encompasses user interface design, or UI design — which isn’t unusual in the UX design world — she also fleshes out her wireframes into prototypes.  While prototyping, she often looks to the internal style guide. This document details a brand’s look in terms of colors and its specifications can get very nitty-gritty.  The do’s and don’ts of using the ubiquitous Facebook logo (Sample don’t: “Don’t make the ‘f’ logo the most distinctive or prominent feature of what you’re creating.”)  UX designers often have to present wireframes and prototypes to key stakeholders which can include internal teams and external clients.  Presentations usually involve demonstrating how a new product will work UX designers can trace features and formatting back to research and analysis.  Communicating isn’t just about finished designs “there’s a lot of communication with developers to make sure that your ideas can be implemented in their timeframe,” Dobe says “They have a whole other set of priorities and obligations on their end.” Granja usually makes two wireframes to discuss with them: one of the ideal design and one of a “happy medium” design that will be quicker to code UX designers don’t just collaborate with developers, though. They routinely meet with product and project managers a steady flow of conversation keeps everyone in the loop about whether a design is for mobile or desktop And ultimately, UX design hinges on collaboration.   “The common thread [through] every day is that I’m constantly working with several different teams.” But no matter how impressive one’s background is, UX designers often begin their careers in lower-level positions. Gaining experience in entry-level roles like graphic designer or UX researcher may be necessary before one can become a UX designer A UX designer is in charge of making products like apps and websites as accessible as possible By applying user-centered design principles and researching customer needs UX designers create products that are intuitive and easy for users to navigate internships and four-year degrees all help one become a UX designer An early-career professional may also need to gain experience in entry-level roles like UX researcher and graphic designer before securing a position as a UX designer Those who acquire skills in areas like researching designing and communicating can enjoy a rewarding career in the field of UX design The Cedar Mill Farmers Market is in full swing this summer, bringing together community members and local farmers every Saturday in the parking lot of the Sunset Mall. Founded in 1999 by Leilani Esping the market has grown to include over 30 vendors providing an impressive selection of fresh produce and artisan goods The spirit of friendship is very much alive underneath each canopy because we know we’re doing something good for the community,” said Nicolas Amaro owner of Granja Nuevo Horizonte in Gales Creek Nicolas grows an assortment of seasonal vegetables They are proud to never use any chemicals or synthetic pesticides in their produce The Amaros have been vendors at the Farmers Market since 2009 They arrive at around 6 am to set up for the day starting their business at 9 am when the market opens and closing down at 2 pm the best part about coming to the market is the opportunity to interact with its vibrant community “We love to hear our customers!” he exclaims “They’re happy and they really appreciate what we do I say it’s like the gas for us to keep going.” She is a representative of Kiyokawa Family Orchards in the Hood River Valley a third-generation orchardist whose grandfather emigrated from Japan in 1905 The orchards grow over 100 distinct varieties of apples Laura greets each patron with a smile and knows many of them by name She is also in charge of the aquatics program at the Sunset Athletic Club “I see a lot of kids [at the market] that I taught who are 18 now and I taught them when they were five or three,” Laura recalls Laura found a way to reconnect with her swim students through the Farmers Market what are you doing here!?’… and so it’s been really fun and rewarding.” Debra Pearce of Nuts About Berries enjoys catching up with her return customers as well “It’s really good seeing the same faces year after year after year,” she says She and her partner Jon appreciate the cozy atmosphere of the Cedar Mill Farmers Market as it allows for a more personalized shopping experience their stand transitioned to a touchless format; elements of that layout were kept to prevent cross-contamination one can find many unique items made by small businesses specializes in homemade soap and skincare products Binah Goldman creates her soaps with all-natural oils and butters “Everything is formulated to be super gentle,” she explains “I get a lot of positive feedback from people including folks with skin issues like eczema and psoriasis.” Binah is a resident of Cedar Mill herself the Cedar Mill Farmers Market remains a cornerstone of the growing community and excitement to the Safeway parking lot each Saturday The market is more than just a place to shop for groceries—it’s a celebration of the local vendors whose passion for sustainability and dedication to their work are apparent in any interaction Whether you’re searching for farm-fresh foods the Cedar Mill Farmers Market is a treasure trove waiting to be explored It is open every Saturday from 9 am to 2 pm through October 14 August 2023 Articles New Businesses August 2023 Cedar Mill Farmers Market Brings Diverse Vendors History in the News August 2023 Business News August 2023 CPO News August 2023 August 2023 Road News Washington County News Cider Festival update In first year, 988 crisis line helps more people than ever Nature & Gardens August 2023 Cedar Mill Community Club updates Park News August 2023 School News August 2023 Legislative news Community News August 2023 Library News August 2023 Click here to search for articles from 2003-present. NOTE: you're not submitting personal information so you can ignore the warning Click next to the Search button to enter text Useful information for the Cedar Mill community Do you have news or events or know of something that you would like to see covered in the Cedar Mill News Send us an email 1000 printed copies are distributed throughout the Cedar Mill area. Printing courtesy of Pacific Office Automation Opinions expressed in this publication are not necessarily those of the advertisers Sohrab Darabshaw | Posted on April 5 Rio Tinto Group recently agreed to sell a 55% majority stake in its La Granja copper project in Peru to First Quantum Minerals Ltd The deal involves the First Quantum paying U.S The hope is that the sale will allow the project to progress even faster It should also allow the Rio team to concentrate more on their flagship copper mining developments in Mongolia and the United States this will allow First Quantum to access and progress the site’s potential development via a feasibility study La Granja is a challenging ore body situated in the high-altitude region of Cajamarca in Northern Peru the site boasts an indicated and inferred mineral resource of 4.32 billion tons at 0.51% copper This represents a tempting deposit for any copper mining firm With Rio Tinto and First Quantum Minerals now joining forces to develop the site given their combined development capabilities the world could have a major new source of copper very soon Stay ahead of the ever shifting copper market. MetalMiner Insights provides comprehensive copper price forecasting and AI feeds which give copper buyers a competitive edge over the competition. Schedule a demo. It was 2006 when Rio Tinto acquired the La Granja Project from the Government of Peru The company carried out an extensive drilling program in the intervening years significantly expanding the declared resource and information regarding the ore body Rio Tinto also partnered with host communities and national and regional governments during that time Further developing the La Granja will significantly strengthen Rio Tinto’s copper portfolio Copper is essential to the global shift toward clean energy. The metal has a range of applications for everything from electric vehicles to wind turbines. Moreover, S&P Global expects copper demand to increase significantly over the next decade the growing demand for copper in the clean energy sector and under-investment in copper mining has led to concerns about a looming shortage Many analysts continue to warn of the potential for severe scarcity in the future The La Granja announcement comes on the heels of the commencement of underground mining at Oyu Tolgoi in Mongolia in March 2023 Analysts forecast that Oyu Tolgoi will become the fourth-largest copper mining operation in the world by 2030 Open pit mining at Oyu Tolgoi has been operational since 2011 it’s producing ore from 1.3 kilometers below the surface of the Gobi Desert Not sure why you should trust MetalMiner’s metal price forecasting? Take a look at our track record. analysts expect the mine to produce around 500,000 tons of copper per year Meanwhile, Rio Tinto’s top honchos believe that the short-term outlook for copper is “healthy.’ This is despite global stockpiles trending down and mine disruptions eroding supply from Latin America Get monthly tricks of the metal trade which can result in significant cost downs, savings the and margin erosion. Schedule a consult for MetalMiner’s Monthly Outlook and see a free sample copy. Filed under: Commodities, Manufacturing, Non-ferrous Metals, Supply & Demand MetalMiner helps buying organizations better manage margins generate cost savings and negotiate prices for metal commodities The company does this through a unique forecasting lens using artificial intelligence (AI) technical analysis (TA) and deep subject matter expertise © 2025 MetalMiner. All rights reserved. | Cookie Consent Settings & Privacy Policy | Terms of Service is the chief financial officer of the Peruvian-themed La Granja Restaurants Latin Style Magazine and Bank of America named Bartra one of 12 Hispanic Women of Distinction in 2015 honored Bartra for being a “strong advocate and supporter of children’s educational issues both in the United States and her homeland in Peru.” She and La Granja weer also recognized for efforts to honor war veterans on Veteran’s Day a Latino and Puerto Rican youth organization as well as helping to fund scholarships for students attending Palm Beach State College Bartra is also the owner and managing partner at Pinnacle Insurance & Financial Strategies and food distributor Products and More About your company: La Granja was established in Aruba in 1993 My father and brother opened their first restaurant in Florida in Margate in 1995 Our rotisserie chicken and our flavor had a great acceptance La Granja restaurant is mainly corporate owned mostly of them owned by our [former] top employees How your business has changed: It was not easy at the beginning Competing with big food chains of restaurants is not easy at all I have a wonderful team; we get the job done with great passion “Conquer today and do it tomorrow all over again.” First paying job and what you learned from it: At 17 I developed a clothing brand with my aunt in my hometown in Peru I learned that discipline and determination are key factors to reach your goals Best business book you ever read:¿Estás Comunicando Best piece of business advice you ever received: My father always uses the quote great problems and great solutions.” I always see “the glass half full.” Go around the problem and find the positive part of it What do you tell young people about your business Be persistent and have a lot of discipline What do you see ahead for Palm Beach County It has grown a lot since then; new business and many home communities have been built Palm Beach County is very family oriented; great place to raise your kids Where we can find you when you are not at the office: Supervising one of my restaurants The best part of my job is that I manage my time so in the afternoon I can spend quality time with my two kids and my husband Favorite smartphone app: I have an iPhone 6 plus What is the most important trait you look for when hiring: The most important thing is their willingness to work The collapse of Silicon Valley Bank in March put the spotlight on how US banks account for bonds and other tradable financial assets. SVB reclassified $8.8 billion of assets to avoid writing down their value, according to Chicago Booth’s João Granja—but a surge of withdrawals forced the bank to liquidate $21 billion of government bonds at a big loss which accelerated the run and forced US federal banking regulators to step in Many more lenders may be at risk of failure because American banks have substantial hidden losses in their portfolios banking authorities need to take a look at banks’ application of the rules for marking assets as “hold to maturity,” meaning they have the intent and ability to let the securities mature and cash them out for full value it doesn’t need to update those assets’ prices as interest rates fluctuate Securities not marked HTM are classified as “available for sale,” and their value on the books does ride the market Granja analyzed federal call-report data for 2021 and 2022 US lenders reclassified billions of dollars of securities as HTM assets during those two years financial institutions accounted for 45 percent of their $6 trillion in securities The research finds that when the US Fed started tightening the money supply in 2022 there was an increase in the share of commercial banks’ securities classified as “held to maturity,” which shielded banks from having to report the securities’ value fluctuations as interest rates changed “These numbers suggest that banks actively sought to insulate their balance sheets and statements of income from declining market prices,” Granja writes Were weak banks attempting to purposefully ‘hide’ potential future losses that might expose their frail capital and liquidity positions?” The Fed’s cycle of monetary tightening led to a 10–30 percent drop in the price of bonds and similar securities That spelled losses for banks holding those assets Granja estimates that US banks were able to avoid recognizing $175 billion in losses on securities simply by slapping the HTM accounting label on them this reclassification camouflaged a modest amount of damage—but at a few banks would have eroded more than half of their capital The study finds evidence that these banks may not have really had the intent and ability to hold on to these securities His analysis indicates that less-stable banks—those with smaller capital bases more securities that were exposed to interest-rate risk and a higher share of customers whose deposits were above the $250,000 Federal Deposit Insurance Corporation guarantee—were more likely to reclassify securities during 2021 and 2022 This suggests that weaker banks were attempting to hide potential future losses that would expose their shaky financial positions He calls for stricter external scrutiny from auditors and regulators to strengthen financial stability “Bank supervisors may have forgotten how dangerous these interest-rate risks might be in the financial system because rates have been so low for such a long time,” he says The Fed’s recent stress tests on banks did not include scenarios with higher interest rates arguing that if losses on securities portfolios had been obvious earlier supervisors could have forced banks such as SVB to shore up their finances before it was too late it’s possible that future losses will come to light sooner and corrective action can be taken faster to salvage a bank that is in danger,” Granja says João Granja, “Bank Fragility and Reclassification of Securities into HTM,” Working paper Thanks to Cristine for sending the sad news. La Granja de Oro was located at 1832 Columbia Road NW La Granja’s Falls Church, VA location still seems open Updates when we learn what becomes of the Adams Morgan space 2022 (GLOBE NEWSWIRE) -- Customers asked and they listened: La Granja Restaurants is opening a location in Lakeland due to overwhelming demand "Customers asked many times for us to expand to Lakeland We're proud to announce it's finally happening!" said Claudia Bartra The Lakeland location will open in approximately three months, adding to the over 50 locations in Florida. In the meantime, customers can travel to La Granja's other locations in preparation for their grand opening by finding their closest location dine-in and third-party delivery apps as options More information about the expansion is forthcoming soon La Granja Restaurants has been known for their excellent and affordable Latin cuisine including the 1/4 Chicken meal and delectable sides such as white rice Their first location opened in Aruba in 1993 before expanding to the United States in 1995 La Granja's focus on customer service and commitment to delicious cuisine keeps the restaurant chain growing Fantastic Dining for Everyone in the Family La Granja proudly offers meals that are affordable and filling Some of the most popular additions include fried plantains Families can also order platters with food for four or more people pork or steak and pair it with your choice of sides honor traditional Latin American cuisine and are homemade for a comfortable Their famous chicken dishes are offered in whole half and quarter sizes and come with rice or beans Customers can also snag chicken wings with fries or their Boneless Breast Special beginning Their seafood specials include whole snapper criollo fish and shrimp paired with sides like Fried Calamari and Seafood Rice La Granja also offers sandwiches and fajitas Lunch or dinner guests will want to come back again and again for their homestyle and accessible meals La Granja also offers a wide selection of wine and beer to pair with your favorite meal they are guaranteed great dining and plenty of mouthwatering choices Call (954) 589-1967 or visit www.lagranjarestaurants.com to learn more about the upcoming expansion Contact Information: Jeffrey Brown P.R. jeffb@topofgoogle.com This content was issued through the press release distribution service at Newswire.com exploring how rave culture and tribal festival gatherings have changed the way we think about hotels Now he's opened a farm on Ibiza - but keep it quiet.. that doesn't host foam parties or sell own-brand day-glo running vests La Granja is embedded in the beetle-dry pine forest in the hills way above San Antonio gone-to-ground Ibiza; the setting for something fresh: a members'-club agriturismo with nine bedrooms in a restored farmhouse he's fired up by an emerging evolutionary hybrid: the hotel as meeting point for a nomadic creative class drawn not so much by Panton chairs and polished concrete as by a shared experience - whether that's psychedelic electronica biodynamic food or vinyasa yoga - stage-managed with the attitude and makeshift playfulness of the festival scene When it came to creating his own place to stay it was unlikely to feature anything so traditional as valet parking which began as a pop-up hotel but solidified into something permanent with esoteric performances at its low-key beach club Then Claus was approached by the owner of an Ibizan farmhouse 'He had the notion of turning it into an art hotel but I thought this could be different.' has thick rocky walls crusted with brilliant white plaster There's a Moorish attitude towards light and shadow cool as a badger's burrow; open a shutter and the sunlight will almost knock you over Walls are painted the dark grey of unfired clay wooden tables and stools resembles a Dutch still life A writing desk at La GranjaSteve HerudA shady indoor seating areaSteve HerudDays at La Granja are lazy Claus's two sons play tag around the garden and aubergines seem to inflate like balloons as you watch Coco the pig provides the raw materials for Andy the farmer's compost-tea which bubbles in a barrel by a ramshackle shed Boxes of leafy radishes and heritage tomatoes are carried up to the kitchen who spins tales of his mezcal adventures around Mexico and serves the rootsy spirit with worm-salt-strewn plates of fruit 'I used to spend winters in Koh Tao which is where Alex Garland wrote The Beach,' says Claus the main guy describes his utopia as a beach resort for people who don't like beach resorts just a black rock and a gate that slid back mysteriously for people to walk through and see the other side of Ibiza - the one before all the craziness happened.' A Citroën Méhari will be kitted out with blankets and picnic baskets; a local newspaper editor has been asked to source a library of Ibizan literature A bedroom at La GranjaSteve HerudLeftfield ideas have always flourished on this island casually explains the science behind a process that rejigs water's molecular structure to speed up plant growth he talks of how more places like La Granja will emerge in the future as megacities bulge ever bigger and people flip the way they work and holiday On the weekend I'm here, a hit-and-run festival collective, Habitas, have landed from LA bringing in musicians and wellness practitioners A breathing-meditation class blurs into a DJ session under the trees our instructor's white fedora bobbing up and down as ululating African chants rise over fluid basslines 'It's like how the island was 30 years back,' reckons one Ibiza regular La Granja is helping it reinvent itself with a wholesome hedonism and valuable thinking space Claus moves through the grounds like a pied piper observing the crowd and making connections lagranjaibiza.com; from about £18,265 for three nights (sleeps 18) This feature first appeared in Condé Nast Traveller October 2016 Granja Rinya will present its Homenaje and Carmen cheeses winners at the 2023-2024 World Cheese Awards Granja Rinya will attend Alimentaria with the endorsement of the recognition of its cheeses at the 2034-2024 World Cheese Awards held in Norway The Capricho Homenaje soft cheese won the super gold medal as one of the best cheeses in the world and the award for the best cheese in Spain while the 1.1 kg Homenaje won the silver medal the international competition endorsed the Carmen range of cheeses with a silver medal for the sheep’s milk cheese cured with black garlic and bronze medals for the Manchego DOP Viejo and the artisan mature LC (cow-sheep) Capricho Homenaje is a soft cheese made solely with pasteurised cow’s milk that quickly matures It’s presented in a 150 g format wrapped in paper and in a display box containing six units It has a highly characteristic flavour and aroma inspired by Swiss cheese Tomme Vaudoise and it’s a unique product in Spain This cheese gains character and creaminess throughout its shelf life (21 days) in such a way that it almost becomes spreadable by the end of it but it’s advisable to remove it from the fridge 10 minutes before consumption The 6th International Conference on NeuroRehablitation 2024 in “La Granja de San Ildefonso” in La Granja The conference is scheduled to have a significant number of exoskeleton and wearable related topics The event is targeted towards clinical rehabilitation professionals The primary goal of the conference is to discuss novel approaches challenges and solutions for wearable robotics The main organizers behind the event are the Shirley Ryan AbilityLab and the Center for Clinical Neuroscience HLM General sponsors of ICNR include Fourier Intelligence The online registration deadline is October 11, 2024. For more information and to sign up, visit: https://2024.icneurorehab.org ICNR 2024 Tracks include wearable robotics AI and Computational Methods in Rehabilitation and Neural Interfaces and Stimulation All tracks are potentially relevant to exoskeleton technology expect discussions and presentations on topics like: and curated articles from around the web delivered to your inbox every week About Exoskeleton Report Authors & Contributors Contact Us Terms and Conditions Privacy Policy Disclaimer A major overhaul is planned for the Dyckman Farmhouse in Inwood the structure is the oldest remaining farmhouse in Manhattan and currently houses a museum a group created to raise funds to preserve the farmhouse which will improve accessibility through ADA access to the property and ADA-compliant restrooms the project will install the addition of a ramp from the street onto the property to provide improved access to the park space It marks the first major refurbishment for the house since 2003 “The Dyckman Farmhouse Museum Alliance is looking forward to the project as it will create greater accessibility to the museum and include more usable space for the organization and its visitors,” said Don Rice President of the Dyckman Farmhouse Museum Alliance Board Located at the corner of 204th Street and Broadway the Dutch Colonial farmhouse served as home to the Dyckman family for nearly 100 years The restoration project will be managed by the Historic House Trust of New York City in partnership with NYC Parks Funding for the renovations has been provided by Mayor Bill de Blasio Manhattan Borough President Gale Brewer and City Councilmember Ydanis Rodríguez No timeline has been provided for the project Fridays and Saturdays for self-guided tours “The Dyckman Farmhouse Museum is thrilled to undergo this restoration project in order to continue its mission of preserving the amazing historic and cultural site for the Inwood community,” said Meredith Horsford Executive Director of the Dyckman Farmhouse Museum Alliance “We are eager for these changes that will enhance the visitor experience and contribute to our goal of engaging our community by connecting the past with the present.” For more information, please visit www.dyckmanfarmhouse.org La Granja Dyckman recibirá renovación mayor Está prevista una reforma importante para la Granja Dyckman en Inwood Construida alrededor de 1784 por William Dyckman la estructura es la casa de campo más antigua que queda en Manhattan y actualmente alberga un museo un grupo creado para recaudar fondos para preservar la granja anunció el proyecto de renovación planificado que mejorará la accesibilidad ADA a la propiedad y asegurará que los baños cumplan con la ADA el proyecto instalará la adición de una rampa desde la calle hacia la propiedad para brindar un mejor acceso al espacio del parque Marca la primera reforma importante de la casa desde 2003 “La Alianza del Museo de la Granja Dyckman espera ansiosamente el proyecto ya que creará una mayor accesibilidad al museo restaurará la histórica casa de campo e incluirá más espacio utilizable para la organización y sus visitantes” presidente de la Junta de la Alianza del Museo de la Granja Dyckman Ubicada en la esquina de la calle 204 y Broadway la granja colonial holandesa sirvió de hogar a la familia Dyckman durante casi 100 años El proyecto de restauración será administrado por el Fideicomiso para Casas Históricas de la ciudad de Nueva York en asociación con Parques de la ciudad de Nueva York Los fondos para las renovaciones fueron provistos por el alcalde Bill de Blasio; la presidenta del condado de Manhattan No se ha proporcionado un cronograma para el proyecto viernes y sábados para visitas auto guiadas “El Museo de la Granja Dyckman está encantado de someterse a este proyecto de restauración para continuar con su misión de preservar el increíble sitio histórico y cultural para la comunidad de Inwood” directora ejecutiva de la Alianza del Museo de la Granja Dyckman “Estamos ansiosos por estos cambios que mejorarán la experiencia del visitante y contribuirán a nuestro objetivo de involucrar a nuestra comunidad conectando el pasado con el presente” Para más información, por favor visite www.dyckmanfarmhouse.org