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Upgrade now. This Haiku sign was installed Sunday at the top of Maliko Gulch heading east A new Haiku gateway welcome and aloha sign was installed on Sunday morning along Hana Highway Haiku resident and former Haiku Community Association President Gregg Blue offered private funding and the design In coordination with the state Department of Transportation HCA Treasurer Tim Wolfe and contractor Duarte Lima of Acacia Construction installed the double-sided sign at the top of Maliko Gulch heading east The Haiku gateway sign is a way to welcome visitors and residents into the community and to share aloha as they leave A grant information session for Maui County’s recycling grants program will be at 2 p.m In light of increased property values driving up tax payments the Maui County Council’s budget committee has .. Copyright © 2025 Maui News Publishing Company LTD | https://www.mauinews.com | 100 Mahalani Street American anthropologist has contributed to primate conservation in the Atlantic Forest and trained almost 80 Brazilian researchers visited the forests of Caratinga in the Brazilian state of Minas Gerais for the first time She was immediately captivated by the northern muriquis (Brachyteles hypoxanthus) Muriqui males are the same size as the females and they are a peaceful species in direct contrast with the displays of dominance she had witnessed years earlier among baboons in Africa Strier has divided her time between her work as a professor at the University of Wisconsin–Madison in the US and her field research in Brazil Perhaps because of the warm welcome she received from the primates in Brazil (humans included) or the tropical climate of the Atlantic Forest compared to the harsh winters of the northern USA she says she feels more alive when she is in Minas Gerais Caratinga locals have promised to throw her a party next year to celebrate her 40th year studying the endangered apes about which little was known when she first arrived The population has undergone a remarkable recovery although their numbers are still below the level needed to ensure the continuity of the species She was president of the International Primatological Society until the turn of the year and she returned to Brazil in February to help formulate a recovery plan for the muriquis in São Francisco Xavier a village in the municipality of São José dos Campos She also visited the Ibitipoca Commune in Lima Duarte to see the muriquis there for the first time in more than six months She spoke to Pesquisa FAPESP via video call from her accommodation in Vila do Mogol where she teaches and lives with her biochemist husband and their cat Tell us about the meeting in São Francisco Xavier in early February São José dos Campos city hall got in touch with me because they had information about muriquis in the surrounding forests They had read my book Faces in the Forest and wanted my opinion it’s the biologists who start field research projects but in this case it was the local community that called on us to develop a primate conservation plan I came to continue my long-term research and help my colleague Fabiano de Melo and to continue discussing this project in São Francisco Xavier Now in February we’re getting started again with everyone wearing masks and keeping 2 meters apart The first thing to do will be to better understand primate populations in the region—their size and connections between them—as the basis for a conservation plan The work here is an independent supplement to my long-term research It’s one of the longest primate studies in the Americas which has allowed me to train nearly 80 Brazilian scientists a rural entrepreneur called Renato Machado from here in Ibitipoca contacted me and my colleagues Fabiano who is the director of INMA [the National Institute of the Atlantic Forest] and Leandro Jerusalinsky from the CPB [the National Center for Brazilian Primate Research and Conservation] There was a small population here—just four males—and later there were only two meaning they needed a lot of help to recover I had already observed in Caratinga that it is the females that leave the group and the males that stay only males remain and no more offspring are born which is called Muriqui House and is managed by Fabiano and Fernanda Tabacow we looked for females from other isolated populations—almost all of them had at least one trying to leave—and brought them to live and reproduce with the males of the group The plan is to create two groups to facilitate genetic exchanges between the females Another parallel project is working to restore the forests where the muriquis live What was primate research like in the 1980s when you first started studying muriquis for many biologists who go into anthropology primates are good models for understanding human social behavior and evolution; since we are primates I was looking for an animal model to study how ecological variables could influence social behavior and group hierarchies most of the studied primates were from Africa and Asia Howler monkeys were the only ones from the Americas studied in the field in any depth was narrating a film about muriquis made by Russell Mittermeier [American primatologist] for the WWF [World Wildlife Fund] and asked if I wanted to see the film before it was distributed I discovered that almost nothing was known about the behavior of the species I needed to find out what they eat and the seasonality of their diet and test theories developed for other primates I went to Caratinga with Mittermeier and he introduced me to the muriquis What was your first encounter with them like First I smelled them—a nice cinnamon smell because they’re vegetarians I was really curious to see how they behaved and from the very beginning it was very clear in my mind that everything I learned could be applied to their conservation I only stayed in Caratinga for a short time that year but later I came back to spend 14 months collecting data for my PhD research you had to go into town and wait in line to use the only phone in the area The muriquis were different—they weren’t typical primates In 1994 I published a paper called “Myth of the Typical Primate,” which offered a new perspective on primates in general there is no size difference between the sexes Males can’t threaten females because they aren’t bigger than them An animal’s diet is calculated based on the proportion of each food type it eats So if the largest proportion of their diet was leaves I realized that leaves were important to their diet because their entire behavior was focused on what they preferred to eat: fruits and flowers This changed our interpretation of how diet should be used to interpret behavior Did anything else differ to theoretical predictions When I was 19 and doing my undergraduate degree I spent six months in Africa participating in a study on baboons I had an idea of what a typical primate should be like Male baboons are twice the size of females It is the males that migrate between groups looking for a mate to reproduce with are monomorphic—there is no size difference between the sexes Males cannot threaten females because they aren’t bigger aggression between group members is very low compared to other primates Their sexual behavior is very open: males and females mate in front of each other or one female mates with several males in a row she leaves—the males don’t chase after her it’s the male leader who decides who to mate with and when Do you know why they are so different to other primates We have collected their feces and extracted estrogen and other hormones to understand their reproductive biology We determined that females have 21-day cycles and gestation lasts 7.2 months We have also seen that females leave their birth group at the beginning of puberty and take some time to integrate into other groups We’ve done paternity analyses using feces and monitored changes in the population and its demography What changes occurred in the population you followed there were two groups of about 50 individuals but after about 20 years we decided to study the entire population the population grew from 50 individuals to 356 One of the changes we observed is that the animals always stick together when the group is small but they spread out more when the size of the group increases and competition for food grows because they can’t all fit in the same trees As the population grew and the available forest diminished This behavior spread within a group and between different groups—it was probably an adaptive response to the limited space Personal archiveStrier has been watching muriquis from the trails of the Caratinga reserve for 40 yearsPersonal archive But the years 2014 and 2015 were very dry in Brazil There was almost an electricity crisis because the dams ran out of water but a wave of yellow fever killed more than 30 individuals—10% of the population—in six months The muriquis experienced mortality like never before the population has continued to dwindle in the last five years which is still five times what it was when I started The population growth was a direct result of favorable demographic conditions: births every three years the owner of the farm where the Caratinga reserve is located banned hunting and helped preserve the forest The forest was protected and the population was able to grow his family created an RPPN [Private Natural Heritage Reserve] named in his honor you published an article about the muriquis’ limit of resilience We still don’t know what this species’ limits of resilience are What the muriquis are demonstrating is that given a chance they are capable of adjusting their behavior and adapting to challenging conditions we have to pay attention to these changes and gather more information I am monitoring how the muriquis in Caratinga adapt over time Do you have a personal relationship with the muriquis The muriquis from Caratinga are like people I know although less so nowadays because the last individual of the original group died a few years ago; they can live for over 40 years The animals were my companions for many years and I have no doubt that they recognized me Many other researchers have visited over the last few decades but when I go into the forest with a visitor they try to hug me and threaten the other person What was it like when you first went to the interior of Minas Gerais to begin your research Later I returned with funding and a research visa during my PhD Brazil has a system that requires foreign researchers to have a counterpart—a local collaborator a professor from UFMG [Federal University of Minas Gerais] He welcomed me into his laboratory and made it easy for me to get to know people in the area the first director of the Primatology Center in Rio de Janeiro He and others who pioneered the conservation of the Atlantic Forest made me feel so welcome and helped me a lot I met Sergio Lucena at the research base in Caratinga I was studying the muriquis for my PhD and he was doing a master’s on howler monkeys but he later moved to the US and worked at Conservation International He’s now at the GEF [Global Environment Facility] we included Fabiano de Melo as a second collaborator to help with the workload Carla de Borba Possamai and Fernanda Tabacow who started as fellows on the project in 2001 and 2005 This system gave me the opportunity to collaborate with primatology and environmental conservation experts in Brazil and I felt part of a larger group from the very beginning It was a long-term project—I secured the funding but I couldn’t have done anything without my Brazilian colleagues A long-term study lets you see which behaviors are more rigid and which are more flexible 23-year-old woman who came from another country to live alone in the forest for 14 months Many residents came to the research base just to see me; one woman wanted to touch my hair even though there’s nothing special about it Feliciano came by every day to check on me We want to get local people and schools from the regions where we work more involved to maintain communication networks between children and get people interested in preserving and monitoring the areas where primates live We recently carried out a citizen science project with Marcello Nery president of MIB [Muriqui Biodiversity Institute a nongovernmental organization based in Caratinga] to learn more about primates in the reserve We made a calendar with photos of the four monkey species that live in the region and asked residents to write down if they saw or heard any of them We then discovered that we didn’t even need to make monthly visits to collect the data because people could tell us what they saw via WhatsApp Does the unified theory of behavioral ecology mean we can study insects and primates in the same way you can’t study different animals in the same way A good study needs to use a methodology designed for the specific species The principles have a certain continuity because they come from kin selection At least among social species such as primates and ants there is much greater variation in behavior than expected But it is not possible to compare concepts of individuality between primates or other species that do not live as long as primates but primates have more opportunities to learn and respond to novelty related to their lifelong histories and cognitive abilities that make them as interesting as other long-living animals This is one of the major contributions of our research on the behavior of muriquis we are able to see which behavioral aspects are more rigid and which are more flexible This helps a lot when planning population management Along with the greater understanding of behavioral flexibility that we have gained over the years has come the recognition that in most cases we are studying animals that are not in their original environments—their habitats have been altered When I told my colleagues in the US that I was going to study muriquis in Caratinga You’re going to study animals somewhere that has been very disturbed to the point that it’s now just a fragment How are you going to understand their evolutionary behavior?” The northern muriquis came from the old Atlantic Forest in the south of Bahia which is now highly fragmented and deforested This means they now live closer to farms and cities as do the southern muriquis [Brachyteles arachnoides] and are thus at greater risk of being killed by humans But this problem is faced by primates all over the world We have to be careful with our observations because we don’t know exactly what the original conditions were like admitting that we don’t know what to expect Now—and this is since quite recently—we can say with certainty that the northern muriqui and the southern muriqui are different species Genetic analyses show differences between the two species and they may have emerged some 2 million years apart It’s similar to the genetic difference between the chimpanzee and the bonobo in Africa Personal archiveWith Feliciano Abdala in Caratinga Do you have students permanently studying muriquis I have received funding from the National Science Foundation the University of Wisconsin–Madison itself the CNPq [Brazilian National Council for Scientific and Technological Development] and CAPES [Brazilian Federal Agency for Support and Evaluation of Graduate Education] not only to bring over students from the US but also to train and qualify Brazilian students based on the fact that the muriquis represent a part of Brazil’s heritage Many biologists here wanted to study them but weren’t able to do so due to a lack of funding or training Every year two to four students join my study to collect data for their master’s degrees and later carry out their own scientific research to support conservation Contributing in this way gives me immense satisfaction The muriquis have helped train and educate the next generation of scientists Those who started with me on fellowships are now doing their own studies in other places such as the Caparaó National Park [on the border between the states of Espírito Santo and Minas Gerais] Did your work at the International Primatology Society contribute to your research in Brazil My term was supposed to end in August 2020 our conference [at which the president of the society is changed] was postponed I presided over the International Primatology Society for longer than any previous president: five-and-a-half years was president of SLAPRIM (the Latin American Society of Primatology) We worked closely and had a good relationship because we’ve known each other for a long time Brazilian primatologists therefore naturally had a lot of opportunities at the international society because they felt comfortable getting in touch with me one of the society’s vice presidents and its general secretary are Brazilian I was able to help by giving a bigger platform to people who were already very active in the field giving them recognition and promoting their work I have no doubt that one of them will be president in the next few years Brazilians are renowned in international primatology In 2020 you received an important award in Atlantic Forest conservation: the Muriqui Prize so I can’t say I should have won it sooner It made me feel like my Brazilian colleagues are still taking care of me which is a result of the long-term partnerships I have forged with my Brazilian colleagues Have you ever felt like going back to Africa where you had your first primatology experience The only time I went back to Africa was a trip to Kenya a few years ago to speak at a conference I told the audience I had been there as a student when I was 19 and never thought I would return as president of the International Primatology Society I applied for funding to study primates in Asia and I was offered a 14-month scholarship with free language lessons included but I chose to return to Brazil and continue studying muriquis instead I was “married” to the muriquis and never thought about leaving but there I teach and have day-to-day responsibilities at the university Maybe part of the reason I feel more alive here is because right now it’s summer here while it’s zero degrees in my home city © Revista Pesquisa FAPESP - All rights reserved Metrics details Chromobacterium violaceum is a free-living bacillus with several genes that enables it survival under different harsh environments such as oxidative and temperature stresses Here we performed a label-free quantitative proteomic study to unravel the molecular mechanisms that enable C total proteins extracted from control and C violaceum cultures exposed during two hours with 8 mM hydrogen peroxide were analyzed using GeLC-MS proteomics Analysis revealed that under the stress condition the bacterium expressed proteins that protected it from the damage caused by reactive oxygen condition and decreasing the abundance of proteins responsible for bacterial growth and catabolism GeLC-MS proteomics analysis provided an overview of the metabolic pathways involved in the response of C violaceum to oxidative stress ultimately aggregating knowledge of the response of this organism to environmental stress This study identified approximately 1500 proteins generating the largest proteomic coverage of C We also detected proteins with unknown function that we hypothesize to be part of new mechanisms related to oxidative stress defense we identified the mechanism of clustered regularly interspaced short palindromic repeats (CRISPR) which has not yet been reported for this organism previous proteomic profilings disclosed at most violaceum in response to an environmental stress These investigations detected changes in the expression pattern of several genes such as sigma factors and MerR family which are involved in general stress response They also found that exposure to hydrogen peroxide induces the gene expression and mechanisms associated with oxidative stress thioredoxin reductase and peroxidase as well as mechanisms of iron and manganese homeostasis and SOS response The present study investigates changes in the proteome profile under oxidative stress induced by a high dose of hydrogen peroxide; we believe these changes reflect the mechanisms used by this bacterium to cope with the oxidative stress our results provided experimental evidence on hypothetical proteins and thus shortlisting the approximately 40% of the ORFs annotated as hypothetical proteins we used an LTQ-Orbitrap Velos to obtain a substantial comprehensive proteomic profile of an organism that is protecting itself against ROS Given the nature of the experiment at hand these proteins are highly likely to be related to oxidative stress defense violaceum starts to recover from the oxidative stress exposure Each point of the curve represents the mean from three biological replicates ***p < 0.001 (T-test for comparisons between negative control on its respective time) Each column represents the mean value ± SD of 3 separate experiments (column 1: control group; column 2: bacteria cultured with 8 mM of H2O2) (A) Exclusively identified proteins from oxidative stress condition (B) Proteins identified exclusively in control condition 35 ORFs remained as proteins with unknown function: 19 for oxidative stress and 16 under control conditions This shows that new mechanisms related to resistance and/or detoxification processes may be present in C Genome analysis indicates that some of these ORFs (CV_4246 CV_0053 and CV_3599) display operon organization the elevated baseline production of catalase in this bacterium may provide greater resistance to hydrogen peroxide and activation becomes insignificant under stress These proteins may play a role in the protection and refolding of proteins oxidized by hydrogen peroxide Activation of the expression of these genes under such stress restores the phenotype to the organism conferring a characteristic that allows it to migrate in the environment in adverse conditions The above studies indicate that the action of this operon favors adherence to the host greater expression under oxidative stress indicates that these proteins may also play a role in stress response The presence of these proteins indicates the need for greater energy demand to resist damage caused by oxidative stress oxidative stress likely models ribosomal structure not only through interaction with other proteins but also by expressing genes that improve the efficiency of translation under oxidative stress The schematic depicts the CRISPR/CAS locus of C Two CRISPR loci flank the six Cas genes (blue bars) The enlarged diagram shows the clamp formed in repetitive regions containing 28 nucleotides (brown bars) separated by a spacer sequence (green lozenge) Increased expression of five dehydrogenases shows that C violaceum requires an increased energy demand in the intracellular environment to combat stress caused by hydrogen peroxide The detected dehydrogenases produce intermediate in the glycolytic pathway and the citric acid cycle and also produces NADH+ that will feed the oxidative phosphorylation increasing the production of ATP the most representative proteome of this bacterium comprising a coverage of more than 30% of predicted ORFs This proteome representation contributes to a better understanding of general environmental stress acclimation and may be used in further proteome studies for comparison purpose of many hypothetical ORFs that may have their role investigated in further analysis The bacterial strain used in this study was Chromobacterium violaceum ATCC 12472 It was inoculated into Petri dishes with LB (Lysogeny-Broth) agar and incubated at 28 °C for 24 h Individual colonies were aerobically pre-inoculated into 50 mL Falcon tubes containing 5 mL aliquots of liquid LB and submitted to agitation at 200 rpm and 28 °C for 16 hours Pre-inoculated samples were transferred to 250 mL Erlenmeyer flasks in a fresh medium at a ratio of 10:1 (final volume of 50 mL culture) Bacteria were cultivated under the same conditions until reaching the exponential growth phase in an optical density (OD) at 600 nm of 0.4 – 0.5 Control samples remained under growth conditions and for treatment these were added to the 8 mM H2O2 cultures Bacteria were cultivated and treated in three independent experiments In order to determine the effect of hydrogen peroxide on the growth rate of C bacteria were cultivated in liquid LB with or without 8 mM of hydrogen peroxide Growth was monitored by measuring the OD600 of cultures at 15 Growth curve by CFU (colony-forming unity) count was performed Treatment and growth conditions were as described above with plating dilutions varying from 10−5 to 10−7 100 μL of each sample was plated and the bacteria grew for 24 h prior colony counting using Scan 1200 from Intersicence data was normalized to number of colonies in 10−6 dilution Three biological replicates and two technical replicates were used Catalase activity levels were determined with commercial Catalase Assay Kits (Catalogue no USA) according to the manufacturer’s instructions This assay is based on the reaction of catalase with methanol in the presence of hydrogen peroxide The formaldehyde produced is measured upon its reaction with Purpald chromogen which specifically forms a bicyclic compound that changes to colorless to purple color upon oxidation Catalase activity was measured using total proteins extract as described above and was calculated in nmol.min/μg of protein the concentration of total protein extract was used The experiment was performed in three biological replicates A total of 20 μg of protein was submitted to SDS-PAGE 12% in a Mini-Protean electrophoresis module (Bio-Rad) Precision Plus Proteins WesternC Standard (Bio-Rad) was used as marker The gel was further stained with Coomassie Colloidal G-250 (Sigma-Aldrich) the gel was washed 3 × 30 minutes with 100 μL of 50% acetonitrile (ACN) solution and 10 mM of NH4HCO3 pH 8.0 and then dehydrated with 100 μL of ACN for 10 min Disulfide bridges were reduced using 100 μL of 10 mM dithiothreitol (DTT) in 100 mM NH4HCO3 for 30 min at room temperature followed by dehydration for 10 min with 100 μL of 100% acetonitrile and alkylation with 50 μL of idoacetamide (IAM) 55 mM/10 mM NH4HCO3 for 30 min in the dark The gel was dehydrated under the same conditions and dried with Concentrator Plus (Eppendorf) 15 μL of 13 ng μL−1 trypsin solution (Trypsin Promega Sequencing Grade Modified) in 10% NH4HCO3 100 mM and 10% acetonitrile was added to the dried gels in an ice bath for 30 minutes and then incubated at 37 °C for 16 hours Tryptic peptides were extracted from the gel by adding 30 μL of extraction solution (95% ACN and 5% formic acid at 0.1%) and placed in a shaker under low agitation for 15 minutes at room temperature the entire solution was removed and transferred to new tubes and dried with Concentrator Plus (Eppendorf) Prior to being placed in the mass spectrometer the samples were reconstituted in 10 μL of 0.1% formic acid and sonicated for 10 min We used a reversed phase EASY-nano LC system (Proxeon Denmark) coupled to an LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific Each hydrolysate was loaded in two replicates (technical replicates) into an 18 cm fused-silica emitter packed in-house with ReproSil-Pur C18-AQ reversed phase 3 μm resin (Dr Peptides were eluted using a gradient from 100% phase A (5% acetonitrile +0.1% formic acid) to 50% phase B (80% acetonitrile +0.1% formic acid) in 70 minutes; 50% to 60% phase B in 20 minutes; 60% phase B to 100% in 15 minutes and 100% phase B in a further 15 minutes at a flow rate of 200 nL/min Ion mass spectra were obtained in positive mode by automated MS scanning and tandem MS/MS Each MS scan collected in the Orbitrap at m/z 300–2000 and resolution of 60,000 was followed by MS/MS of the ten most intense ions in the LTQ Fragmentation in the LTQ was performed by collision-induced dissociation (CID) with a dynamic exclusion of 60 seconds and normalized collision energy of 35 TFold uses a variable fold-change cutoff that takes into account a theoretical FDR estimator that maximizes the number of identifications Only proteins present in at least two biological replicates were considered for this analysis violaceum were previously inoculated and the treatment in the presence and absence of 8 mM H2O2 was made during two hours as mentioned above total RNA was extracted using RNAspin Mini Isolation Kit according to manufacture’s instructions (GE The synthesis of cDNA was further made using the up-mentioned RNA High capacity cDNA reverse transcriptase kit was used according to manufacturer’s instructions (Applied Biosystems Statistical analysis was performed according to the t-test Results were considered significant for p < 0.05 GeLC-MS-based proteomics of Chromobacterium violaceum: comparison of proteome changes elicited by hydrogen peroxide Chromobacterium violaceum: a review of pharmacological and industiral perspectives Cyanide Formation By Chromobacterium Violaceum Metal solubilization from metal-containing solid materials by cyanogenic Chromobacterium violaceum Chitinolytic Activity in Chromobacterium violaceum: Substrate Analysis and Regulation by Quorum Sensing Isolation and identification of poly (3-hydroxyvalerate)-degrading strains of Pseudomonas lemoignei Identification of Chromobacterium violaceum genes with potential biotechnological application in environmental detoxification Cellulose Biosynthesis by the Beta-Proteobacterium Chromobacterium violaceum and its important metabolites–review The Complete Genome Sequence of Chromobacterium violaceum Reveals Remarkable and Exploitable Bacterial Adaptability Tolerance to stress and environmental adaptability of Chromobacterium violaceum Proteomics Analysis of the Effects of Cyanate on Chromobacterium violaceum Metabolism Proteomic Response to Arsenic Stress in Chromobacterium violaceum Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum The influence of iron on the proteomic profile of Chromobacterium violaceum Management of oxidative stress in Bacillus Iron homeostasis and management of oxidative stress response in bacteria Peroxide stress elicits adaptive changes in bacterial metal ion homeostasis Adaptive response to oxidative stress: Bacteria Primary and secondary oxidative stress in Bacillus Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa Mass spectrometry-based quantitative proteomic analysis of Salmonella enterica serovar Enteritidis protein expression upon exposure to hydrogen peroxide Transcriptional analysis of Mycobacterium fortuitum cultures upon hydrogen peroxide treatment using the novel standard rrnA-P1 Comparative global transcription analysis of sodium hypochlorite peracetic acid and hydrogen peroxide on Pseudomonas aeruginosa The transcriptome response of Neisseria gonorrhoeae to hydrogen peroxide reveals genes with previously uncharacterized roles in oxidative damage protection Transcriptome analysis of the response of Pseudomonas aeruginosa to hydrogen peroxide Transcriptome and proteome analysis of Bacillus subtilis gene expression in response to superoxide and peroxide stress Hydrogen peroxide-induced oxidative stress responses in Desulfovibrio vulgaris Hildenborough Burkholderia pseudomallei Rpos regulates OxyR and the katG-dpsA operon under conditions of oxidative stress Analyzing marginal cases in differential shotgun proteomics Defining the transcriptome and proteome in three functionally different human cell lines Global quantification of mammalian gene expression control A soluble 3D LC/MS/MS proteome of the filamentous cyanobacterium Nostoc punctiforme Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources Two-dimensional proteome reference map of Rhizobium tropici PRF 81 reveals several symbiotic determinants and strong resemblance with agrobacteria Proteomic analysis reveals a virtually complete set of proteins for translation and energy generation in elementary bodies of the amoeba symbiont Protochlamydia amoebophila Re-annotation and re-analysis of the Campylobacter jejuni NCTC11168 genome sequence Oxidative stress: Molecular perception and transduction of signals triggering antioxidant gene defenses Cellular defenses against superoxide and hydrogen peroxide Oxidative stress triggers thiol oxidation in the glyceraldehyde-3- phosphate dehydrogenase of Staphylococcus aureus Superoxide dismutase and oxygen toxicity defenses in the genus Neisseria The Many Faces of Gluthathione in Bacteria Identification of proteins related to the stress response in Enterococcus faecalis V583 caused by bovine bile Oxidative stress in bacteria and protein damage by reactive oxygen species Oxidative stress response in the opportunistic oral pathogen Fusobacterium nucleatum Proteomic analysis of antioxidant strategies of Staphylococcus aureus: diverse responses to different oxidants Specialization of an Exonuclease III family enzyme in the repair of 3′ DNA lesions during base excision repair in the human pathogen Neisseria meningitidis Post-translational modifications of desulfovibrio vulgaris hildenborough sulfate reduction pathway proteins Conjugative DNA transfer induces the bacterial SOS response and promotes antibiotic resistance development through integron activation The DNA-binding activity of the Neisseria gonorrhoeae LexA orthologue NG1427 is modulated by oxidation The peroxide stress response of Bacillus licheniformis Control of bacterial virulence by AraC-like regulators that respond to chemical signals Novel bacterial MerR-like regulators: Their role in the response to carbonyl and nitrosative stress Prokaryotic transcription regulators: More than just the helix-turn-helix motif Beyond gene expression: The impact of protein post-translational modifications in bacteria Transcriptome and physiological responses to hydrogen peroxide of the facultatively phototrophic bacterium Rhodobacter sphaeroides That Controls Twitching Motility and Virulence in Xylella fastidiosa Functional selection of a type IV pili-binding peptide that specifically inhibits Salmonella Typhi adhesion to/invasion of human monocytic cells Ribosome binding proteins YhbH and YfiA have opposite functions during 100S formation in the stationary phase of Escherichia coli Bacteriophages and insertion sequences of Chromobacterium violaceum ATCC 12472 Hydrogen peroxide-mediated induction of the 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enhanced sensitivity and specificity Search engine processor: Filtering and organizing peptide spectrum matches Proteomic parsimony through bipartite graph analysis improves accuracy and transparency Improving the TFold test for differential shotgun proteomics PatternLab: From mass spectra to label-free differential shotgun proteomics Download references The authors are also grateful to Jana Dara Freires de Queiroz for performing the experiments of catalase activity levels Laboratório de Biologia Molecular e Genômica Universidade Federal do Rio Grande do Norte Ciência e Tecnologia do Rio Grande do Norte (IFRN) Universidade Federal do Vale do São Francisco Laboratório de Proteômica e Engenharia de Proteínas acquisition of data and analysis and interpretation of data and drafted the manuscript participated in the design of the study and analysis and interpretation of data carried out the proteomics studies and writing of the manuscript contributed to the study conception and design writing of the manuscript and overall supervision revising it critically for important intellectual content All authors read and approved the final manuscript The authors declare no competing financial interests Download citation Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma. Audio recordings are used to profile ecosystems and identify changes caused by humans Linilson Padovese / USP Graphic representation of underwater sounds recorded at Laje de Santos State Park with a hydrophone built at USPLinilson Padovese / USP Marina Duarte / Puc-Minas Equipment records ambient sounds in an area of the Cerrado in Minas GeraisMarina Duarte / Puc-Minas Diogo Sergio / Wikimedia Commons An area of Caatinga in Rio Grande do NorteDiogo Sergio / Wikimedia Commons She installed microphones both inside a well-preserved section of forest and along its edge, 500 meters from the Brucutu mine, one of the largest iron ore extraction sites in the world. From October 2012 to August 2013, the sounds were recorded for seven-day stretches, with an interval of two months. Explosions, sirens, machine noises, and especially the traffic of heavy trucks, which can number 700 per day at certain times of the year, affected most of the study area. The effects were most intense at the edge of the forest, 25 meters from the road the ore was transported on. There, the complexity of sounds was significantly lower than in the section’s interior, indicative of less species diversity. The sonic richness on the edge of the forest near the mine was also lower than in another much less noisy border area, near a dirt road used by cars. Eliziane Garcia De Oliveira A graphical representation of bird and rain sounds recorded at different timesEliziane Garcia De Oliveira Insect sounds Vocalizations and other sounds made by the animals were more frequent during the day than at night in the forest nearest the mining area while the opposite was true for the more distant area The frequency range in which insects produced their sounds also differed between the first and second locations Most likely as an adaptation to the noisy environment they chirped in a narrower frequency range (lower or shriller than the machine noise) at the forest’s edge near the Brucutu mine while insects in the deeper forest area employed a wider sonic range the researchers report in an article published in 2015 in the journal Biological Conservation such as the toco toucan (Ramphastos toco) and the plumbeous pigeon (Patagioenas plumbea) were only heard in deep forest areas far from the mine Sousa-Lima and his team at the UFRN Bioacoustics Laboratory are working on creating sound profiles of other ecosystems biologist Eliziane Garcia de Oliveira made recordings during both the dry and rainy seasons in an area of the Caatinga (semiarid scrublands) She is investigating the impact of wind power generators on this soundscape and before finding out if anything will change needed to know the natural behavior of the ecosystem bleached vegetation is silent for most of the year as well as tweets and warbles of one or another bird and the Caatinga is transformed: the stridulation of insects intensifies other birds begin to sing and the chatter of toads “The sonic environment becomes complex,” Oliveira summarizes biologist Luane Ferreira compared six acoustic indices with the aural identifications resulting from manually counted sound logs from three areas of Cerrado in Serra da Canastra National Park in Minas Gerais State Graphic representations of the soundscape indices and the logs are then correlated to the recordings published in 2018 in the Journal of Eco-acoustics none of the indices fully captured the species diversity of this tropical environment Music and environment The origin of this new area of ecology formalized in a series of articles presented in 2011 in a special issue of the journal Landscape Ecology in particular the work of American musician Bernie Krause Krause began his career in the 1960s as a studio guitarist and worked with rock bands such as The Doors and The Rolling Stones which introduced synthesizers in pop music and film His career began to change in 1968 when he and Beaver were hired by a record label to make a series of unusual albums would be the first to include long stretches of nature sounds Krause has accumulated about 5,000 hours of recordings including works such as The Great Animal Orchestra Symphony Symphony for Orchestra and Wild Soundscapes written by British composer Richard Blackford in collaboration with Krause and humpback whales mix with those of violins and other orchestral instruments in the work In a commentary published in July 2018 in the journal Biotropica a group of biologists and ecologists from the United States and Brazil reaffirmed the need to increase the acoustic monitoring of tropical ecosystems The text proposes creating a global repository of the recordings “Soundscape recordings provide a permanent record of a particular location at a given time and contain a wealth of invaluable and irreplaceable data,” the group stated Recorders have become cheaper and data storage systems have improved a failure to collect sonic data on tropical ecosystems could present a serious problem for future generations who could benefit from ecology research Brazilian footballer Vinicius Tobias has discovered that he is not the biological father of the child he believed to be his had even gotten a tattoo in honour of the unborn child before receiving the devastating news The revelation came after his ex-girlfriend which confirmed that Tobias was not the biological father of the baby girl Lima shared the news on her Instagram stories Vinicius and I haven’t been together for a while and it turns out Maitê is not Vinicius’ daughter,” Lima explained in a now-deleted post the child’s real father is speculated to be Vinicius Duarte an açaí delivery man with whom Lima had an affair during her on-and-off relationship with Tobias Reports indicate that both Vinicius and Lima had a rocky relationship marked by infidelity on both sides Sources close to the couple revealed that Lima’s involvement with Duarte occurred during one of her breakups with the footballer though the specifics of his actions remain unclear The betrayal has been especially hard on Tobias who had already tattooed Maitê’s name Lima urged the public to stop attacking Tobias clarifying that he had respected her request not to attend the delivery or post anything about the child until the DNA test results were confirmed “We’ve talked things through and settled everything amicably and we both hope to move on peacefully,” Lima stated who was on loan at Real Madrid from Shakhtar Donetsk until the summer of 2024 He spent two seasons with Madrid’s B team since the first team’s non-EU player slots were filled Tobias earned medals for Madrid’s Champions League and Supercopa de España triumphs during the 2023/24 season Tobias is taking time to process the personal setback while continuing his professional journey in Ukrainian football This site uses Akismet to reduce spam. 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