After moving nearly 6,000 miles from Brazil for college Helena Silva Goldbaum discovers community and a calling Editor’s note: This story is part of a series of profiles of notable spring 2025 graduates You can’t chase your dreams if you stand still That’s what Helena Silva Goldbaum realized four years ago Goldbaum wanted her future to be a story of her own volition She sought a college experience that allowed her to explore different interests colleges as an international student — a process she needed to figure out from scratch Arizona State University stood out thanks to its support for international students scholarship opportunities and room to grow she leaped headfirst and moved nearly 6,000 miles to Arizona — alone and I didn’t have connections or know anyone who had done this before,” she says Fast-forward four years, and that move turned out to be a career-defining decision: Goldbaum is graduating as the Sanford School’s Spring 2025 Dean’s Medalist with concurrent degrees in psychology and family and human development a strong network of peers and professors she built along the way As a student in Barrett, The Honors College, she researched at the Emerging Minds Lab and the Child Study Lab co-authored a peer-reviewed journal article and assisted with multiple courses — including CDE 394: Designing Innovative Interventions for Children She also completed a competitive research internship at Harvard a feat which involved countless applications and mock interviews with professors This allowed her to spend a summer exploring how young children make meaning of their environments and understand other people's minds That sense of motivation and curiosity has guided her time at ASU and helped her carve out a future in developmental science she will begin a PhD program in educational psychology with a focus on applied developmental science at the University of Virginia with the goal of designing research that promotes healthy development in children “Helena is a superstar — bright, curious, dedicated, compassionate, caring and highly committed to learning,” said Professor Richard Fabes, professor and director of innovation at the Sanford Harmony Institute “She is eager to become a developmental scientist whose work makes a difference in the lives of children and those who care for and about them.” We caught up with Goldbaum to reflect on her journey what she has learned and where she’s headed next Question: What was your “aha” moment when you realized what you wanted to study?  Answer: During my first year, I started working at ASU’s Child Study Lab, and that experience completely shifted my perspective. I was fascinated by how kids think and how much they’re capable of. I also had an “aha” moment when I took CDE 394: Designing Innovative Interventions for Children, taught by Richard Fabes and Carol Martin That early hands-on experience of making science applicable to the community helped me realize I wanted to pursue research in child development I added family and human development as a second major and started working in research labs I became especially interested in how children’s environments shape their internal motivation and decision-making My thesis focused on how to promote learning and motivation in young children — something I hope to continue researching during my PhD Q: What surprised you most during your time at ASU A: I was really surprised by how clever kids are I also learned how research can be applied more broadly — not just staying in an academic bubble — and how important it is to design studies that make a real-world impact That idea has shaped my entire academic path Q: Were there any faculty members who had a big impact on you She became an incredible role model for me as a woman in science All of them helped me develop my research skills find my voice and see the value of what I bring to the field Q: What’s the best piece of advice you’d give to those still in school A: Don’t be afraid to reach out to your professors especially as a first-year or international student but they’re here because they want to support you Some of my biggest opportunities came from asking questions or going to office hours You never know who will help you find your path or open a new door Q: What’s one change you’d love to see in the world — and how would you use your degree to help make it happen A: I’d love to see more attention on children’s development and how we support it and research should be used to help them thrive — not just sit on a shelf I want to help bridge the gap between research and everyday practice so that what we learn in the lab actually makes a difference in the classroom and at home College of Health Solutions Outstanding Graduate Student Emily Dow has grown into a highly accomplished doctoral student completing her PhD in exercise and nutritional sciences in just three… Medical studies graduate Mia Tarditi aims to support others across a variety of contexts from the club she established as a student, Pre-Health on Poly Dallas Salas has already hit a lot of major milestones He earned his high school diploma through ASU Prep Digital then completed his first degree from Arizona State University when he was… the (literally) ballsy trainers have become a cultural heirloom for frolicking it-kids across the Iberian Peninsula and the world at large Words: Tom George30th September 2024 It’s rare for a cultural phenomenon beloved by millennials to also garner the desire and adoration of Gen Z Surviving the test of the next generation forsaking the trends of yore is quite the conundrum an icon was born in the Iberian Peninsula that would not only take over the globe but would become a timeless go-to for decades in the aftermath of a cultural rebirth within the Balearic Coast and Spain more widely Mallorcan label Camper crafted the Pelotas Ariel: a trainer recognisable for its sleek but rustic vegetable-tanned leather upper which is 360-stitched to an outsole featuring 87 balls on its base Having now sold over 11 million in its nearly 30 year history the Pelotas has proven its place within the classic trainer hall of fame so the first time I saw a pair of Pelotas was in that iconic post-divorce photo of Nicole Kidman,” says creative director Achilles Ion Gabriel ​“When I saw a pair in real life they reminded me of the kind of shoes you wear when you go bowling A new offering from Camper celebrates this perennial legacy, featuring a revamp of one of the earliest incarnations of the trainer. Here, the hand-brushed finish is given to a boxing boot style. That buoyancy of two opponents sparring in the ring is reflected in the youthful and heady energy of the photography by noted Spanish docu and editorial photographer Aitor Laspiur and suck on ice pops under the Spanish sun without a care in the world meanwhile their elders do the daily chores in the background the older folk look no less peppy in their Pelotas I see people wearing the original Pelotas from the 90s,” affirms Achilles I’ll see teenagers wearing the newer styles Everyone loves great quality and great shoes passion and strength of Pelotas and Camper as cultural entities in and of themselves the Mallorcan brand only came to be in 1975 because Camper founder Lorenzo Fluxa’s grandad bringing back the very first sewing machine to the Spanish Island Camper believes so much in its Fluxa craftsmanship that the Pelotas comes with a lifetime guarantee meaning you can get hot and sweaty in the Spanish sun – or English lack thereof – for all your days Back to the story… 20 years after establishing his brand on the island Lorenzo created The Pelotas against the backdrop of Spain’s newfound prestige within the sporting world after the 1992 Barcelona Olympics an event which is said to not only be one of the best Olympics in history but transformed the city and led to major investment and global eyes on Spanish culture The zeitgeisty Pelotas played into this by being both inspired and named after the object that the vast majority of sports stars spend long sweaty hours of their days playing with: their balls – make of that what you will The first Pelotas was evocative of American football pigskins with their aged tan uppers threaded with white laces as well as subtle reflections of the equipment of other sports such as basketball is the unique 87 spherical bubbles dotted across the rubber base that add a natural bounce and sprightliness to every step this is why the Pelotas remains such a major trainer across generations of youth culture ​“We wanted to capture that confidence of being young doing whatever you want,” says Achilles of the campaign the shoe not only presents vitality and zest in its sporty vibes but its spirit of dynamism is written into its culture-defining craft making the perfect template for the new gen of young One thing is for sure: the Pelotas will always be a byword for the bohemians of Spain’s chicest barrios London’s artsiest boroughs and free spirits worldwide By subscribing, you agree to our Terms of Use and Privacy Policy Camper has long been known for its durable, high-quality shoes designed for everyday wear. Traditionally, its materials have been chosen for their resilience, making Camper a go-to brand for work-ready footwear. Recently it has successfully expanded its appeal to a younger audience who might not be as familiar. From its eye-catching “Kobarah” sandals to the innovative CamperLabs line, the brand has shown a keen understanding of diverse age groups and consistently evolved its designs — and, as a result — gained increased popularity. Our Standards: The Thomson Reuters Trust Principles., opens new tab , opens new tab Browse an unrivalled portfolio of real-time and historical market data and insights from worldwide sources and experts. , opens new tabScreen for heightened risk individual and entities globally to help uncover hidden risks in business relationships and human networks. © 2025 Reuters. All rights reserved Petrobras will serve as the operator of the blocks with a 70% stake while Shell will own the remaining 30% stake Brazilian state-owned oil and gas company Petrobras has signed 26 concession contracts for the blocks in the offshore Pelotas Basin The concession contracts are part of the fourth Permanent Concession Offer Cycle from the public session conducted by the National Agency of Petroleum Natural Gas and Biofuels (ANP) in December 2023.  while Shell will own the remaining 30% stake.   In the 4th Permanent Concession Offer Cycle auction Petrobras also obtained three more blocks through a partnership that included Shell and China National Offshore Oil Corporation.  Contracts for those will be finalised later as per the ANP’s timetable.  Petrobras said: “The signing of these contracts is in accordance with the company’s long-term strategy with the aim of diversifying its portfolio; and it also strengthens Petrobras’ position as the main operator of oil fields located in deep and ultra-deep waters potentialising the recomposition of reserves for the future.”  Don’t let policy changes catch you off guard Stay proactive with real-time data and expert analysis As part of the diversification strategy, Petrobras recently expressed interest in acquiring a stake in the Mopane oil discovery made by Galp Energia The Brazilian company could form a partnership for its bid to acquire the 40% stake that is up for sale.   The Mopane field in the Orange Basin’s PEL 83 has drawn interest from more than 12 companies including ExxonMobil and Shell is seeking to sell 50% of its 80% stake in the field which is estimated to hold at least ten billion barrels of oil equivalent.  Most recently, Petrobras teamed up with energy data and intelligence company TGC to advance scientific research and technological development in Brazil’s energy sector.  The companies will develop tech solutions to enhance exploration and production and conduct scientific research to better understand Brazil’s sedimentary basins through geological and geophysical data analysis.  Give your business an edge with our leading industry insights View all newsletters from across the GlobalData Media network Petrobras has signed three more concession contracts for blocks in the Pelotas Basin offshore southern Brazil Petrobras operates all three with a 50% stake Petrobras acquired 29 concession contracts in the Pelotas Basin under the auction O endereço abaixo não existe na globo.com Three oil and gas players – Brazil’s Petrobras and China’s CNOOC – have signed three concession contracts for three blocks in the Pelotas Basin offshore Brazil acquired at the public session held by the National Agency of Petroleum Natural Gas and Biofuels (ANP) in December 2023 During the 4th cycle of the permanent concession offer held by the National Agency of Petroleum Natural Gas and Biofuels (ANP) in Rio de Janeiro on December 13 last year Petrobras (operator) won 29 blocks in the Pelotas Basin out of which 26 were in partnership with Shell Brasil Petróleo and three in consortiums formed with Shell and CNOOC Petroleum The firm signed 26 concession contracts on the same date for blocks in which it holds a 70% stake with its partner According to the  Brazilian state-owned energy giant contracts for the three remaining blocks P-M-1737 in the Pelotas basin have now also been penned the consortium will have Petrobras as the operator and Shell and CNOOC as partners with 30% and 20% stakes “The signing of these contracts is in accordance with the company’s long-term strategy with the aim of diversifying its portfolio; and it also strengthens Petrobras’ position as the main operator of oil fields located in deep and ultra-deep waters potentializing the recomposition of reserves for the future,” explained the firm The expansion of the Brazilian energy giant’s portfolio is aligned with its 2024-2028 strategic plan which puts oil and natural gas at the forefront of further investments positioning them to receive the biggest slice of the planned $102 billion investment pie Petrobras sees oil and gas as drivers of growth, which will propel and fund the energy transition to greener sources of supply. The Brazilian player plans to deploy 14 new FPSO vessels over five years While the pre-salt has been in production for 15 years with more than 30 production platforms in operation and approximately 170 exploratory wells already drilled the Energy Research Company (EPE) expects oil production in the country to peak at 5.3 million boe/day in 2030 Petrobras is also working on multiple projects outside Brazil, including two discoveries offshore Colombia that have raised the gas potential in the Guajira Offshore Basin to approximately 6 trillion cubic feet (tcf) Petrobras also got the green light to acquire a stake in the Deep Western Orange Basin (DWOB) block through a competitive process conducted by TotalEnergies The block is located in deep waters in the Orange Basin where there have recently been significant discoveries by TotalEnergies the consortium will consist of TotalEnergies (operator The Brazilian firm underlined: “The purpose of the operation will be to diversify the exploration portfolio with value generation and is in line with the company’s long-term strategy which aims to oil and gas reserves replacement by exploring new frontiers “The acquisition of the DWOB block in South Africa complies with all the company’s internal processes and governance procedures in line with its Strategic Plan 2024-2028+ and is subject to approval by local regulatory bodies.” Daily news and in-depth stories in your inbox OFFSHORE AND WIND SECTOR If you work in the offshore or wind sector it is essential that you are properly dressed for your job MASCOT offers a wide assortment of safety clothing and safety footwear maintaining or manufacturing wind turbines This season marks a new chapter in footwear as Camper joins forces once again with the vibrant Italian brand Sunnei to reinvent the classic Pelotas Nu Titled “Pelotissima,” this collaboration for Spring – Summer 2025 breathes new life into a style that has been a cornerstone of Camper’s collection since 1996 The partnership between these two eclectic brands promises to blend Camper’s heritage with Sunnei’s forward-thinking aesthetic to create something truly unique established in Milan in 2014 by Loris Messina and Simone Rizzo has quickly garnered attention for its dynamic use of color and an unconventional approach to fashion design The newly designed Pelotissima features an exaggerated chunky creating a contrast with its color-block design These elements highlight the shoe’s commitment to both style and sustainability This collaboration introduces four distinct multicolor styles each reflecting the core values and creative synergy of Camper and Sunnei The vibrant color schemes and innovative design elements perfectly capture the essence of both brands making Pelotissima a standout piece in the global footwear scene enthusiasts will have a chance to snag a pair of Pelotissima through early access the collection will be widely available in Camper stores globally This launch is not just about releasing a new shoe but about setting a precedent for collaborations in the fashion industry where the fusion of distinctive styles creates groundbreaking results This partnership not only underscores the power of collaborative creativity but also sets a new standard for what modern DSCENE is curated as a daily art, design, fashion & lifestyle destination. 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Your Ads Privacy ChoicesIMDb Norwegian offshore seismic vessel player Shearwater Geoservices has started the second acquisition season for the Pelotas Basin multi-client 3D seismic survey offshore Brazil conducted in partnership with Searcher Seismic will be the sixth multi-client 3D project for the joint venture and will utilise the SW Empress vessel to expand the Pelotas survey area beyond 10,000 sq km the project will provide explorers with critical insights into the geological structure of the Pelotas Basin and pave the way for successful hydrocarbon discoveries The survey covers large areas of open acreage expected to be available in the 5th cycle of the Brazilian Open Acreage Release and acreage awarded in the 4th cycle last year The survey area holds significant promise due to its geological connection with the Orange Basin in Namibia and South Africa IMO is bound by the membership of its Flag.. This aspiration could only be achieved whe.. Don't have an account? A UK-Brazil study links childhood trauma to psychiatric disorders in adolescents from low- and middle-income countries A new study by a team of researchers from the UK and Brazil has revealed a strong connection between childhood trauma and the development of psychiatric disorders in adolescents living in low-and middle-income countries The research findings are based on the 2004 Pelotas Birth Cohort which has tracked more than 4,000 children born in Pelotas a city in the southern state of Rio Grande do Sul the study looked at how trauma exposure from childhood affects risk for mental health disorders during adolescence 81% of the adolescents had been exposed to some form of trauma The more different types of trauma experienced the greater the chances of adolescents developing mental health problems one third of all mental disorders were potentially explained by trauma exposure This study builds upon the researchers’ earlier findings in the same cohort which showed that one in three children living in Pelotas were exposed to trauma by age 11 and that trauma was linked to the emergence of common childhood mental health problems Lead researcher Megan Bailey from the Department of Psychology at the University of Bath said: Our findings show that childhood trauma has a lasting impact on mental health particularly in low- and middle-income countries where trauma is very common In combination with previous studies that have also shown these effects in both young people and adults in high-income countries it is clear that childhood trauma exposure is a key risk factor for developing mental health problems across the board Our finding that trauma accounts for at least 31% of all mental health problems by age 18 further underscores this relationship Early interventions are critical to reduce exposure to trauma and help those most at risk of developing mental health disorders In Pelotas, a city-led intervention programme, the Pelotas Pact for Peace, was launched in 2017 to reduce urban crime and violence through health, education, and criminal justice system projects. Early evaluations of this intervention showed lower rates of violent crimes after the intervention was implemented though more research is needed to establish whether it also reduces the prevalence of mental health problems in young people Co-author Dr Graeme Fairchild from the Department of Psychology at the University of Bath said: This study demonstrates that childhood trauma has long-term consequences on mental health – and risk for mental health problems was seen across the board with trauma-exposed youth at increased risk for depression The study highlights the urgent need for prevention strategies to reduce exposure to trauma in low- and middle-income countries and provide more effective support for traumatised children and teenagers Co-author Professor Sarah Halligan from the Department of Psychology at the University of Bath said: Childhood trauma is a robust predictor of poor adolescent mental health and our work demonstrates that youth in low- and middle-income countries like Brazil may be particularly at risk As well as efforts to prevent trauma exposure such as those being undertaken as part of the Pelotas Pact for Peace programme to limit its mental health consequences among youth childhood trauma may have persistent negative consequences across the lifespan Co-author, Professor Alicia Matijasevich from the University of São Paulo Research on the impact of childhood trauma and maltreatment is critically important due to its profound implications for mental health and emotional development These studies not only enhance our understanding of trauma’s far-reaching effects but also provide essential evidence to guide preventive strategies and interventions that can mitigate the long-term consequences of such adverse experiences This new analysis was funded by a studentship awarded to Megan Bailey by the University of Bath and the South-West Doctoral Training Partnership (Economic and Social Research Council) and by projects funded by the Brazilian National Research Council (CNPq; 409224/2021-9) and the Research Support Foundation of the State of São Paulo (FAPESP)/ Economic and Social Research Council/UK Research and Innovation (UKRI; 2023/12905-0) GSFC to start calcium nitrate manufacturing India to become a global hub for Artificial Intelligence says Modi Syngenta acquires Italy-based biologicals company Chandra Asri launches circular food packaging solutions for Indonesian market BASF completes sale of its construction chemicals business to PE firm Covestro buys DSM's RFM business for €1.61 billion Aramco, Sabic supplies blue ammonia for zero carbon project in Japan The minister said that the country is moving towards the gas-based economy which is not only clean and efficient fuel but will also help in reducing the country’s dependence on imported crude oil Kesar Petroproducts begins commercial production of new fertiliser plant Privi Speciality Chemicals posts Q4 FY25 consolidated PAT at Rs. 66.52 Cr Enviro Infra Engineers secures new order worth Rs. 76.96 Cr Gujarat Chem & Petchem Conference 2025 to discuss “Efficient and Sustainable Water Treatment for Chemical Plants” Gujarat Chem & Petchem Conference 2025 to highlight latest development in Lab Innovations, Applications, and Sustainability on May 9 Archean Chemical Industries posts consolidated Q4 FY25 PAT at Rs. 53.53 Cr Ami Organics Q4 FY25 PAT up at Rs. 62.48 Cr Yasho Industries posts Q4 FY25 consolidated PAT at Rs. 5.03 Cr Aether Industries reports Q4 FY25 consolidated PAT at Rs. 50.29 Cr Sadhana Nitrochem posts Q4 FY25 consolidated profit at Rs. 4.10 Cr Associate Vice President - Project and Process The Sprint to the Summit: Unlocking Lab Efficiency through Digital Transformation Forbes Marshall - Need For Ongoing Improvement Delivering on the Promise of Prescriptive Maintenance Moving from Manual to Engineered Palletization The guiding role of fluidized and spouted bed technologies in particle building processes Addition of new capacity is projected to result in YoY growth of approx The new E-GRADE unit will bolster Huntsman's portfolio by providing high-purity New window film range combines advanced R&D and focus on sustainability address global demand for eco-friendly heat management solutions for residential The growth in India’s plastics exports during FY 2024–25 was driven by strong performances across several key product categories The quarterly performance is driven by improved refining and marketing margins Wanhua Chemical signs a Joint Venture agreement with Petrochemical Industries Company With a production capacity of more than 780 tonnes per annum the plant is poised to achieve an annual carbon emissions reduction of approximately 9,000 tonnes First pilot plant deployment of Amogy’s low ruthenium content catalyst marks milestone in scaling ammonia cracking for clean hydrogen production The newly commissioned facility will liquify hydrogen produced by Olin for trailer shipments across the U.S Marsa LNG will be one of the lowest carbon intensity LNG plants in the world Linde is the main supplier of industrial gases to Samsung's complex at Pyeongtaek Ballymore has a total daily gross production capacity of 75,000 barrels of oil per day and 50 million cubic feet of gas per day Empowering industries for complete digital transformation and flexible integration This acquisition will enable Yokogawa to strengthen its DX-related business for the delivery of cloud services A strong performer in its industry at the core of secular and future automation trends With a strong focus on fostering innovation and collaboration Startup Mahakumbh will lay the foundation for the next wave of entrepreneurial success Startups operating in focus areas which mainly include natural gas Twenty shortlisted startups will undergo a 12-week accelerator programme Carbon tax is ‘killing manufacturing’ in UK AdvanSix files patent infringement lawsuit against Arxada group companies EU advances toy safety regulations to protect kids from toxic chemicals Impact of reciprocal tariffs on India’s chemical sector is expected to be minimal: CareEdge SOCMA statement on reciprocal tariffs and US manufacturing strategy Subscribe To Our Newsletter & Stay Updated Performance of chemicals (group-wise) for 2011-12 to 2018-19 Projection of production, capacity & export of chemicals for 2019-2025 Indian Chemical News is an important online resource for news technology updates and interviews with prominent leaders in the chemical and petrochemical space.Indian Chemical News is a media company with interests in online publishing and industry events related to the chemical and allied sectors Get Indian Chemical News top stories every morning in your email inbox Shearwater Geoservices has started its second season of acquisition for the Pelotas Basin multi-client 3D seismic survey offshore southern Brazil, using the SW Empress vessel. The project, which is the sixth multi-client 3D campaign for the Shearwater/Searcher Seismic joint venture, will expand the Pelotas area coverage beyond 10,000 sq km. Areas to be covered include large swathes of open acreage expected to be made available in the 5th cycle of the Brazilian Open Acreage Release, and other acreage awarded last year in the 4th cycle. According to Shearwater, results should help de-risk exploration in the basin, which has a geological connection with the hydrocarbon-proven deepwater Orange basin offshore Namibia and South Africa. Parece que a página que você está procurando não está disponível. The event will begin at 11:00 a.m . at the Palacio de la Música, with free admission for the public. After the official reception and the intervention of the Grand Master, a conference dedicated to the cuisine of the Vega Baja will be offered and the awards and distinctions will be presented, among which the following stand out: The event will conclude with a musical performance by the Marta Andréu Academy of Music and the traditional family photo. At 2:30 p.m., the celebration will continue with a Gala Banquet in the restaurant on the first floor of the Casino de Torrevieja, enlivened by DJ Elías. The menu, which will be priced at €50 per person, includes soup with ball, complete turkey stew, starters, dessert, glass of cava and drinks. Places for the meal are limited and require prior reservation by cash payment or bank transfer to the Brotherhood’s account before April 22. The Brotherhood invites all residents and visitors to join this event, which combines tradition, recognition and the most authentic flavor of Torrevieja. Δdocument.getElementById( "ak_js_1" ).setAttribute( "value", ( new Date() ).getTime() ); Review of Camper Fall 2024 Ad Campaign by Art Director Emanuela Amato and Photographer Aitor Laspiur with models Queenie, Astrid, Manyuon, Mario, Pep, Wei, Patricia Camper celebrates its signature Pelotas shoes with a beautifully joyful and nostalgic campaign shot by photographer Aitor Laspiur Nearing the 30th anniversary since their creation in 1995 and now celebrated with vintage-inspired new versions, Campers’ Pelotas take their name from the Spanish word for ball, in reference to their original inspiration by vintage hand-stitched soccer balls. In the campaign, however, this word is given a clever double meaning through the brilliantly provocative tagline “Still Pelotas. Still got balls.” The imagery, however, is quite wholesome. Shooting on analog film in Montserrat just outside of Barcelona, Laspiur draws on his typical practice of capturing intimate family portraits with a documentary feel. His soulful shots are alive with the joy of youth and country life, capturing an intergenerational chosen family as they soak up the summer, from dancing through sprinklers to cruising on bikes down the mountain. Camper’s ads are always fun, clever, original, and personal, and this latest example is no exception. It’s rare for a brand to create campaign imagery that feels so invitingly warm and genuine, let alone a brand that just focuses on shoes – Camper isn’t like other brands though, and it has the balls (sorry) to show us that. Camper Creative Director | Achilles Ion GabrielPhotographer | Aitor LaspiurArt Director | Emanuela Amato Videographer | Really SorryModels | Queenie, Astrid, Manyuon, Mario, Pep, Wei, PatriciaStylist | Paolo ZagoreoHair | Xavi Garcia, Franx De CristalMakeup | Vicent GuijarroManicurist | Barbara MattelCasting Director | Gaze CastingLocation | Montserrat, Spain In Brazil, US major Chevron and Brazilian state giant Petrobras lead the pack in terms of acreage offered in the latest round. Chevron won 15 blocks in total, while Petrobras partnered up with the London-headquartered major Shell to secure 29 blocks, three of them including Chinese state giant CNOOC International as a partner. In Uruguay, Shell, Argentinian state player YPF, and independents APA Corporation and Challenger Energy Group are present. Even though a new wave of licensing has come to this frontier basin, earmarked investments are still low. The minimum work commitment required by contract in Brazil for the recently awarded tracts is only seismic research, with companies not required to drill any wells. The great advantage of the Pelotas Basin is that it has the largest depocenter along the entire margin, the likely major hydrocarbon kitchen that may have fed numerous oil and gas fields on the northernmost margin of Brazil. This system is ubiquitous on the continental margins of the South Atlantic Ocean. The sole discrepancy between these regions lies in the age of the principal source rock, which may vary between the Aptian and Turonian periods. Analyst, Upstream Research, Brazilflavio.menten@rystadenergy.com Analyst, Upstream Research, Brazilbruna.joia@rystadenergy.com Client Success Manager, Brazilgabrielle.ferreira@rystadenergy.com Vice President, Upstream Researchaatisha.mahajan@rystadenergy.com (The data and forecasts in this column are Rystad Energy’s, and the opinions are of the authors.)  proof that 1995 was one of the finest years on record25 September 2024The premiere of the first Toy Story movie the chart-topping success of Coolio’s Gangsta’s Paradise British athlete Jonathan Edwards breaking the (still unbeaten) world triple-jump record in one colossal 18.29-metre leap: 1995 was an iconic year And while the passing of time has shown that some of the year’s biggest moments have proved a little more dubious than others (Val Kilmer’s saucy batsuit in Batman Forever is best left in the past) the longevity of these pieces of pop culture is proof that true classics have the kind of staying power that keeps them relevant for decades and nowhere can this be seen better than with Camper’s Pelotas the distinctive design from the Mallorca-based shoemaking brand was originally influenced by vintage hand-stitched sports balls (Pelotas means “balls” in Spanish) the most notable signature of this ready-for-anything style was its unique 87-ball rubber outsole which provided unparalleled cushiony-soft comfort – winning the shoe a legion of fans across the world And now Camper is reintroducing the shoe for a new generation (Spoiler: it’s stronger and more stylish than ever.) Available in both a boot (inspired by a ’90s archival design) and shoe silhouette all styles feature that distinctive ball-centric rubber outsole 360-degree-stitched to a premium leather upper each pair comes with a lifetime guarantee – an additional assurance of the enduring quality and durability of these shoes Camper has always been committed to sustainability carefully selecting materials that minimise environmental impact without sacrificing quality Believing in durable products that stand the test of time the brand not only creates great shoes but also meets the highest standards of social and environmental responsibility proving that value and values aren’t mutually exclusive What you’ll notice in this new drop of styles is an evolved Y2K design vibe degradé leather uppers with a hand-brushed finish in shades of brown and black that enhance the silhouette and sporty style of the original These can all be seen showcased in the accompanying campaign shot by Spanish photographer Aitor Laspiur in Montserrat national park outside Barcelona The photos celebrate the quintessentially communal focusing on the love of Pelotas as stylish Because whether you were around in 1995 or you just appreciate what that year gave us nearly three decades later icons such as the Pelotas – or world-record triple-jumps Shop the Pelotas collection at Camper.com From outdated to new memes: How recent discoveries in Namibia’s Orange Basin are shaping exploration strategies for Brazil’s Pelotas Basin The father of modern macroeconomics John Maynard Keynes wrote that “the difficulty lies not so much in developing new ideas as in escaping from old ones” This issue is the beating heart of exploration which is built upon both confounding the expectations of our predecessors and doing it before the competitors The received wisdom of memes are the ideas that explorers use as the glue to hold disparate or uncertain data together in a framework required to define risk and val­ue But as the statistician George Box almost said; all memes are wrong they can equally be disastrously misleading the understanding of the deepwater hydrocarbon system had become wrapped in a cluster of ideas that led most of the industry astray until Shell and TotalEnergies drilled Graff and Venus re­spectively in 2022 The memes had evolved in the da­ta-poor atmosphere of uncertainty where the absence of evidence was all one had these have now been passed through the excoriating fire of an extraordinarily successful drilling campaign and many pre-existing models have been eviscerated in the process becoming fitter in the process to deep water passive margin exploration in general so these are the new exploration memes that will take us forward to the next frontier – the Pelotas Basin of Brazil Hydrocarbon systems have never been so vulner­able to critical thinking-driven analysis – not because we have particularly new ideas about how the key risk elements combine or interact but because recent deepwater drilling experience and 2024 technology have crushed the uncertainties in our exploration mod­els letting the memes evolve and become fitter When Richard Dawkins coined the term “meme” to mirror “gene” like geologic models – earth visions in our imaginations that are capable of replicating and evolving to better fit or mitigate increasingly available data Models of deepwater gravity-driven clastic sedimentation adapting through observation and experience Such as embracing the “contourite” meme – be­coming mixed systems and changing the way turbidite flows are understood to deposit sediments in deep wa­ter settings Bryan Cronin’s brilliant recent work in the Ghanaian margin a number of exploration memes of received “truths” had grown over the decades of exploration evolving in a survival of the fittest sense to work around the available data these had conflated with each other to cast a negative light on all aspects of the hydrocarbon system off the shelf Some of these memes included that the inner basin had no source rock and further outboard the outer basin had no sand with no evidence that this was or wasn’t the case Or even against the evidence such as the thick source rock on the oceanic crust in DSDP361 drilled in 1974 Total Energies’ drilling of Venus and Shell’s drilling of Graff in 2022 represented a Chicxulub evolution event to deepwater exploration memes as the historically negative models in Or­ange went to the flames and sneaky preadapted memes be­came better fit to the new data the dying memes were not built of unwarranted concerns but the negative baggage they brought was not supported by any data These memes were self-replicating received biases For an excellent series on biases in exploration analysis a great starting place is Marc Bond’s LinkedIn posts we appre­ciate that the clastic sediments are mixed systems have re-thought their water depth of deposition rethought the thermal history and thermal blanketing that supports the results of Seismic Thermometry and generates oil in the Aptian confirmed the trapping potential created during syn-drift SDR to MORB transitions and counter regional dip mega-trap creation and re-imagined the potential for inno­vative mixed system slope traps alchemically turning the old high risk “frontier well” terror meme into the low-risk quiet confidence of the future a “quick look” data post-stack time migrated (in 2.5 D mode) and depth converted line that was available for interpretation before the last shot of the 2024 Pelotas MC 3D programme is compared at the same depth and horizontal scale to its near conju­gate margin a 2023 MC 3D pre-stack time migrated and depth converted section from beyond the outer high in Namibia had acquired a number of hydrocarbon system memes that were not helpful: Mud rich delta This foldout lights the blue touch paper that will immolate those memes and allow fitter memes The meme that the Pelotas and Orange Basins are “not at all similar” is the first dinosaur on the Barbie as the transi­tion from SDR to oceanic crust is present on both sections in mirror order The Aptian source rock is visible on either margin as is the outboard counter regionally dipping over­lying soft clastic fan of Aptian / Albian age – the ampli­tude on the foldout is just a function of processing maturity in these two examples The Venus play in Orange = Venus play in Pelotas That sections above the Lower Cretaceous are different is obvious and thanks to dynamic topography The Namibian section comprises 3 km of Upper Creta­ceous instability-induced mass transport deposits whilst the Brazilian margin comprises a well-bedded Cretaceous and Tertiary sequence this is of secondary impor­tance as both isopach’s over the Aptian are the same in the same water depth and possessing the same crustal structure illustrating that the proven Aptian hydrocarbon maturity in Orange is quite reasonably exactly that in Pelotas That the overlying Upper Cretaceous confined channel system in Pelotas is also still present and clearly imaged on fast-track data is one would imagine Searcher has been blessed by being able to play a role in the collection of six huge multi-client 3D datasets across the new play fairways in the South Atlantic – five with our amazing partner Shearwater Sharing observations from these datasets as soon as we can with the industry via our weapon of choice; GEO EXPRO Magazine Now it’s time to review the exploration memes that collided in the Orange Basin find the evidence-rooted ideas that actually work Henk KombrinkHenk.Kombrink@geoexpro.com+44 77 8899 2374 Sales DirectorIngvild Ryggen CarstensSales Enquiries+47 974 69 090 Edited by Javier Pérez Jimenez2025-01-14 13:42:12 Jan 14 (JIT) - Cuba's high command of Basque pelota has designed a critical route for this sport in the current year that includes both international and domestic tournaments The route will begin with a training base that will serve as preparation for the World Frontball Championship scheduled for Mexico City from March 2 to 9 This training will be completed in the Aztec capital from February 11 to 28 as informed to JIT the national commissioner and president of the Cuban Federation of Basque Pelota and Racquetball the jai alai team will move to Mexican territory to take part in the League of Nations which will be held at the same time as the world championship event Both competitions will be qualifiers for the absolute world championship of the sport scheduled for 2026 and the Pan American Games of Lima 2027 Cuban teams in these modalities maintain aspirations to qualify for these events with a full team The agenda on foreign courts is completed with the participation in the under-22 world championship they will attend to be among the first eight places which also grants direct tickets to the world tournament and the continental multisport event will take the Antillean pelotaris to Mexico in October also to achieve the classification of the whole group the national games are scheduled for the pioneer always to be held in San José de las Lajas enter your e-mail address in the box below Vulnerable Rep. Mary Peltola (D-AK) advanced to the general election on Tuesday joining three contenders for the general election in which voters will rank their preferences The Associated Press called the race for Peltola as well as Nick Begich and Nancy Dahlstrom over an hour after polls closed at midnight Peltola came in first with 50.9% of the vote followed by Begich with 26.9% and Dahlstrom with 19.9% as of 1:11 a.m and a fourth candidate will be selected to compete with Peltola Alaska is one of two states that use ranked-choice voting in which voters rank the candidates first through fourth If a candidate gets a majority of most first-choice votes in the initial count votes are reallocated as candidates are eliminated Peltola won in 2022 thanks to ranked-choice voting and Republican infighting that split the party’s vote between two candidates Begich had said he would withdraw from the race if he came in behind Dahlstrom Metrics details The aims of the study were to: (a) describe BMI-for-age trajectories in children up to four years of age; (b) evaluate the association between prepregnancy maternal BMI and the BMI-for-age trajectories Data from 3218 (75.3% of the original cohort) children from the Pelotas 2015 Birth Cohort were analyzed Prepregnancy BMI (kg/m2) was measured on the perinatal interview Z-scores of BMI-for-age were calculated for children at three months Trajectories were identified using a semi-parametric group-based modeling approach Multinomial logistic regression was used to test the association between prepregnancy BMI (weight excess: BMI ≥ 25 kg/m2) and BMI-for-age trajectories were identified and represent children in the “increasing” “stabilized” and “risk for weight excess” group A total of 196 children (7.1%) belonged to the group that was at risk of weight excess Adjusted analyses showed that children whose mothers presented prepregnancy weight excess had 2.36 (95%CI 1.71; 3.24) times more risk of belonging to group “risk for weight excess” when compared to those children whose mothers presented underweight/normal weight before pregnancy The risk of weight excess in children up to 4 years of age were greater in mothers who presented prepregnancy weight excess Prices may be subject to local taxes which are calculated during checkout The data underlying this article were provided by “Pelotas Birth Cohort, 2015” conducted by Postgraduate Program in Epidemiology at Universidade Federal de Pelotas. Information on how to access the data can be found at: https://www.epidemio-ufpel.org.br/site/content/home/ Vézina-Im L-A, Nicklas TA, Baranowski T. Intergenerational effects of health issues among women of childbearing age: a review of the recent literature. Curr Nutr Rep. 2018;7:274–85. https://link.springer.com/10.1007/s13668-018-0246-x The GBD 2015 Obesity Collaborators. Health effects of overweight and obesity in 195 countries over 25 years. N Engl J Med. 2017;377:13–27. https://doi.org/10.1056/NEJMoa1614362 Hinkle SN, Sharma AJ, Kim SY, Park S, Dalenius K, Brindley PL, et al. Prepregnancy obesity trends among low-income women, United States, 1999–2008. Matern Child Health J. 2012;16:1339–48. http://link.springer.com/10.1007/s10995-011-0898-2 Leonard SA, Rasmussen KM, King JC, Abrams B Trajectories of maternal weight from before pregnancy through postpartum and associations with childhood obesity. Am J Clin Nutr. 2017;ajcn158683. Available from: https://doi.org/10.3945/ajcn.117.158683 Castillo H, Santos IS, Matijasevich A. Relationship between maternal pre‐pregnancy body mass index, gestational weight gain and childhood fatness at 6–7 years by air displacement plethysmography. Matern Child Nutr. 2015;11:606–17. https://doi.org/10.1111/mcn.12186 Cadenas-Sanchez C, Henriksson P, Henriksson H, Nyström CD, Pomeroy J, Ruiz JR, et al. Eur J Clin Nutr. 2017;71:1200–5. https://www.nature.com/articles/ejcn201762 Albuquerque D, Nóbrega C, Manco L, Padez C. The contribution of genetics and environment to obesity. Br Med Bull. 2017;123:159–73. https://academic.oup.com/bmb/article/123/1/159/3930933 The genetic and environmental influences on childhood obesity: a systematic review of twin and adoption studies Mameli C, Mazzantini S, Zuccotti G. Nutrition in the first 1000 days: the origin of childhood obesity. IJERPH. 2016;13:838. http://www.mdpi.com/1660-4601/13/9/838 Dalrymple KV, Flynn AC, Seed PT, Briley AL, O’Keeffe M, Godfrey KM et al. Associations between dietary patterns, eating behaviours, and body composition and adiposity in 3‐year‐old children of mothers with obesity. Pediatr Obesity. 2020;15. Available from: https://doi.org/10.1111/ijpo.12608 Hallal PC, Bertoldi AD, Domingues MR, Silveira MF, Demarco FF, da Silveira MF, et al. Cohort profile: the 2015 Pelotas (Brazil) birth cohort study. Int J Epidemiol. 2018;47:1048. https://academic.oup.com/ije/article/47/4/1048/4591650 https://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight Obesity and overweight World Health Organization (WHO). Anthro for personal computers, version 2, 2007: Software for assessing growth and development of the world’s children. Geneva: WHO; 2007. Avaliable in: http://www.who.int/childgrowth/software/en/ WHO child growth standards based on length/height Nagin DS Group-Based Modeling of Development Group-based trajectory modeling in clinical research and hyperactivity on the path to physically violent and nonviolent juvenile delinquency and timing of complementary food introduction are associated with infant weight gain Taveras EM. Childhood obesity risk and prevention: shining a lens on the first 1000 days. Child Obes. 2016;12:159–61. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876524/ Interventions for childhood obesity in the first 1,000 days a systematic review Risk factors for childhood obesity in the first 1,000 days: a systematic review Ogden CL, Carroll MD, Kit BK, Flegal KM. Prevalence of childhood and adult obesity in the United States, 2011–2012. JAMA. 2014;311:806. https://doi.org/10.1001/jama.2014.732 Yang Z, Huffman SL. Nutrition in pregnancy and early childhood and associations with obesity in developing countries: early nutrition and obesity. Matern Child Nutr. 2013;9:105–19. https://doi.org/10.1111/mcn.12010 Trajectories and determinants of weight gain in two cohorts of young adult women born 16 years apart weight and body mass index: a systematic review Agreement between self-reported pre-pregnancy weight and measured first-trimester weight in Brazilian women Effect of correcting for gestational age at birth on population prevalence of early childhood undernutrition Download references This article is based on data from the study “Pelotas Birth Cohort 2015” conducted by Postgraduate Program in Epidemiology at Universidade Federal de Pelotas with the collaboration of the Brazilian Public Health Association (ABRASCO) The first phases of the 2015 Pelotas (Brazil) Birth Cohort was funded by the Wellcome Trust (095582) Funding for specific follow-up visits was also received from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS) and Children’s Pastorate sponsored follow-up at twenty-four months; and FAPERGS – PPSUS and the Bernard van Leer Foundation (BRA-2018-178) for the fort-eight months follow-up PCH and ADB worked in the fieldwork and data collection of the 2015 Cohort TRF and OAAL contributed to the conception and design of this study BPN and GIM conducted the statistical analyses and all authors contributed to the interpretation of findings TRF conducted the research for related literature and wrote the initial draft of the paper All authors contributed to subsequent versions critically reviewed the paper several times The authors declare no competing interests Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law Download citation DOI: https://doi.org/10.1038/s41366-023-01422-1 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Brazil — Petrobras has acquired 29 blocks in the Pelotas Basin offshore southeast Brazil under the ANP’s 4th Permanent Concession Offer Cycle The partners’ proposed minimum exploratory program was another factor in the awards A total of 193 blocks were auctioned in Brazil’s Dec 192 under concession and one under production sharing agreement "signaling strong optimism for the industry," according to Wood Mackenzie.  The total amount in bonuses was US$85 million Karoon and CNOOC will operate blocks in the Santos Basin Brazilian operator Elysian acquired 122 onshore blocks Too many automated requests from this network The “Most interesting Basin in the World” ball has been batted across the Atlantic regularly for the last 30 years Early deep-water success in the Campos Basin of Brazil then across to the Guyana-Suriname Basin and finally the latest success in the Orange Basin of Namibia and South Africa This rally of ever bigger discoveries has occurred as explorers ventured into ever deeper water and is set to continue as courageous companies are lining up to enter offshore Uruguay the Orange Basin plays have unique and novel properties but exploration for these defining characters can be sought not only within the same basin but also where similar conditions of formation have reproduced the plays: across the Atlantic on the Conjugate Margin are the Orange Basin plays really only for the courageous the ultra-deep-water counter regional dipping Cretaceous sand fans and channels of the Venus play might seem exotic – but still that is only because the steady progression into deeper water only now allows us to access this inherently low-risk play: well-sorted sands sitting on mature source rock in counter regional structures covered with several kilometres of clay the contourite-constrained slope channel traps of Graff are only now being recognised as seismic imaging improves and more deep-water mixed system discoveries are being made: high-quality winnowed constrained channel turbidite sands updip of mature thick source rock with contourite drift top and lateral seal even though direct analogues to the discoveries of Venus and Graff respectively in the Orange Basin are recognised they both have the potential for huge reserves in good reservoirs – key for deep-water “advantaged” resources which can be developed and brought to market quickly It has been reported that the Venus discovery could contain in excess of 13 Bboe which would make it the world’s largest deep-water field The two discoveries in Namibia have the resource potential of the entire Guyana-Suriname or Tano Basins Similarly, on the conjugate margin in Uruguay, a prospective resources study for leads and prospects identified offshore Uruguay published ahead of the Venus discovery identified analogous deep-water opportunities each with best estimate resources in excess of 1.4 Bboe In both of the Orange Basin plays the source rock is Aptian in age sitting on oceanic crust and deposited in a restricted marine basin This basin formed not long after drift began and marine transgression switched the SDR (seaward dipping reflector) machine off and allowed Penrose Oceanic crust to form These Aptian basins are likely to be relatively shallow water (see DSDP 361) and anoxic allowing the preservation of organic material The same conditions of shallow water-restricted marine basins would have formed on both sides of the proto–Mid-Atlantic ridge and indeed clearly visible in the Pelotas basin offshore Uruguay which can reach up to 3,000 m in thickness has the same characteristics as the Orange basin equivalent unit The same diagnostic characteristics of a good quality mature source rock (Davison et al., 2018) are observed in Uruguay These sequences can be mapped along the Uruguayan margin at the base of the slope and have Direct Source Indicators (DSI’s) as ubiquitous low frequency type IV AVO characteristics indicating the presence of thick organic rich source rocks Although it is straightforward to map out the isopach of sediments on top of this Aptian source rock and high grade the areas with more than 3,000 m of sediment due to a lack of well penetrations the geothermal gradient is an uncertainty It has been widely reported that the geothermal gradient at reservoir level over oceanic crust at Venus-1 was high It has also been rumoured that the geothermal gradient at TD (Total Depth) of Raya-1 (with TD at Oligocene where a lack of faults that connect the Cretaceous source rock with Cenozoic reservoirs as well as thick Paleocene shales prevent hydrocarbons from migrating towards Cenozoic reservoirs) was similarly high the isopach of the extensive gas-hydrate layer (forming a Bottom Simulating Reflector (BSR)) offshore Uruguay has been mapped to derive a base BSR-geothermal gradient which has in turn been used to model thermal maturity at Aptian level The results indicate widespread hydrocarbon generation with present day oil generation in the ultra-deep-water realm Such oil generative basin modelling is unsurprising in the area as there are direct analogies to the proven Namibian margin and Direct Hydrocarbon Indications from fluid inclusions in the Cretaceous sequence of Lobo and Gaviotín wells and microseeps detected in surface geochemistry studies all of which demonstrate a working petroleum system The Venus discovery in Namibia comprises two thick fans totalling gross 137 m of sand deposited directly onto the Aptian source rock These fans are deposited in counter regionally dipping structures where the distal end of the fan provides the pinch out The dip is controlled by plate subsidence and loading and so structures and traps are ubiquitously huge Whilst it is speculated that these fining upward units are deep-water sandstones of turbidite apron-fan association it is also possible that these comprise relatively shallow water pro-delta deposits the Aptian basin had not flooded back over the SDRs of the Orange Basin outer-high (when that happens in the Albian there is a possible carbonate build-up play established there) suggesting water depths of less than 200 m at the basin floor Fluvial systems could carry mature sands out through gaps or canyons between SDR edifices in the outer high This is very likely to be similar to the depositional system of the Uruguayan Margin where thick fan-like sequences are mapped on 3D datasets in addition to intricate aggrading channel belts prograding out onto the basin floor Contourite drifts and other mixed systems abound on this margin Younger sediments of Albian and Late Cretaceous age are winnowed and sculpted by contourites to create plays and traps as yet untested on a series of terraces up the slope provide exciting and new low risk traps on this margin the role of sea bottom currents flowing parallel to the shore (contourite currents) has been recognised in their interactions with turbidite gravity driven currents which create ‘mixed’ or ‘hybrid turbidite-contourite systems’ contourite currents can winnow the turbidites leaving the turbidite enriched in coarse clastic content These hybrid systems have been associated with significant discoveries such as the Mamba and Coral fields offshore Mozambique with an accumulation in the order of 80 TCF (13.8 Bboe) It has been reported that the secondary objective at the Graff-1 well was a play associated with a mixed or hybrid system (Bijkerk et al., 2021) and from reported results it is inferred that an accumulation was encountered in this play with a potential of over 3 Bboe An analogous play has been identified by ANCAP offshore Uruguay where impressive contourite drifts confirm interaction between turbidites and contourite currents With energy security and energy independence at the top of many nations’ political agenda it has become urgent to find new significant accumulations Deep-water plays keep defying paradigms and yielding the largest discoveries These discoveries are to be found in deep-water not only on the West African Coast in analogous situations to Venus and Graff but also on the South American Atlantic Margin – where similar depositional environments and geology have created comparable low risk traps The game is on for explorers to take the analogue set of plays in deep-water and match the discoveries on the path to exploration glory and the world’s future low-carbon advantaged energy The collaborative series between TWA and SPE Student Chapters “TWA Student Chapter Spotlight,” aims to feature award-winning SPE student chapters from around the world Each article in the series will spotlight a student chapter and share some of the chapter’s main events and activities recently held Universidade Federal De Pelotas Presidential Award for Outstanding Student Chapter 2020 the SPE Universidade Federal De Pelotas (UFPel) Student Chapter continued to engage in the dissemination and exchange of knowledge among professionals and those interested in the oil and gas industry While chapter members engaged with the society in many ways and volunteered at nursing homes the programs that had the greatest impact were the “VII Petro-Sul,” which is an academic week of petroleum engineering Petro-Sul is the largest technical event in the southern region of the country and professionals from the industry participated in the event The event aimed to disseminate technical knowledge gave the students a glimpse into the industry and emphasized the need for interdisciplinary training for a petroleum engineer Several oil and gas companies participated and supported the event We received positive reports from students who and workshops and networked with oil and gas professionals Our second technical event in 2019 was the IV Meeting The organization of this event arose from the need to transmit knowledge and show all participants the new technologies and updates about the industry The short courses offered many students their first opportunity to work with petroleum engineering software and highlighted the importance of this skill in the job market every effort was made so that at the end of the second day our participants made the most of the knowledge disseminated during the lectures and courses given by renowned oil and gas professionals from all regions of Brazil we believe that we will be hosting events encouraging increasingly greater relationship between professionals and students of petroleum engineering The Way Ahead is written by and for young professionals in oil and gas var(--popover-size));border-radius:var(--chakra-radii-l3);--popover-z-index:var(--chakra-z-index-popover);z-index:calc(var(--popover-z-index) + var(--layer-index 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recipes{.css-107anqq{font-family:var(--chakra-fonts-body);color:var(--components-article-header-hero-author-text-color);font-size:var(--chakra-font-sizes-xs);line-height:1;width:-webkit-fit-content;width:-moz-fit-content;width:fit-content;}html.teamusa-theme .css-107anqq{text-transform:uppercase;}@media screen and (min-width: 80rem){.css-107anqq{font-size:var(--chakra-font-sizes-md);line-height:md;}}}by Brendan Rourke Mateos will compete in the newest pelota variant Salvador plays individual fronton and doubles frontenis with Omar Team USA is not sending athletes to compete in the doubles trinquete discipline The general rules are similar to racquetball's rules One team starts play by serving a ball toward a large front wall except for a line indicating the height at which the ball must hit above The returning team then must hit the ball off the front wall again to avoid losing the point Play continues as the teams alternate hitting the ball towards the front wall Players are allowed to play the ball off any other wall as long as the ball eventually hits the front wall above the height line before bouncing it is then allowed to bounce only one time off the ground before the other team must attempt to perform the same action.A point is earned by the serving team when the opponent allows the ball to bounce twice hits the ball out of play (in some variations there is no out-of-play due to the court’s design) or interferes with the other team’s opportunity of hitting the ball A team loses its serve if the returning team bests the opponent in the same fashion.Federation tournament matches are played in three sets and are usually played up to 15 or 21 points The third set is shortened similar to the shortening of final sets in beach volleyball matches Doubles Trinquete — This variation will be played with two teams of two wielding broad wooden racquets called paletas and using a rubber ball filled with gas that generates more bounce and speed than leather balls But the most distinguishing feature of this variation is the court The trinquet is a court designed with a front wall a glass wall on the right (for spectators) a wall on the left that has a dugout built into it (for athletes) The dugout is covered with a downward-sloped roof There is no chance to hit the ball out of play as the athletes are enveloped in the playing area this variation will appear most like it.A picture of the court is below frontball players do not use the racquets shown in the below image reCAPTCHA helps prevent automated form spam. The submit button will be disabled until you complete the CAPTCHA. [data-focus]){outline-width:var(--focus-ring-width 2px);outline-offset:var(--focus-ring-offset solid);outline-color:var(--focus-ring-color);outline:none;}.css-puhhl:is(:focus-visible [data-focus-visible]){box-shadow:none;outline-width:3px;outline-style:solid;outline-color:var(--semantics-focus-light);outline-offset:3px;}.css-puhhl:visited{color:var(--components-rte-light-link-color);}.css-puhhl:visited.css-puhhl:visited:is(:active [data-state=open]){color:var(--components-rte-light-link-color-active);}@media (hover: hover){.css-puhhl:visited:is(:hover [data-disabled]){color:var(--components-rte-light-link-color-hover);}}.css-puhhl.css-puhhl:is(:active [data-state=open]){color:var(--components-rte-light-link-color-active);}@media (hover: hover){.css-puhhl:is(:hover white);}}© 2025 United States Olympic & Paralympic Committee the Mesoamerican Ballgame Association is working to revive a 3,000-year-old team sport called ulama (or tlachitl in Nahuatl and pok-ta-pok in Mayan) is played by teams of two to four who hit a rubber ball using only their hips across specific points of the opposing team’s side of the court to score eight points first The game was played both for fun and for spiritual purposes which was widely popular across ancient Mesoamerica was banned in the 16th century following the arrival of Spanish conquistadors in the region Conquistadors who viewed the sport witnessed players hitting a heavy ball using only their body and not sustaining injuries perceiving them as witches and demon worshippers The oldest ballcourt discovered is located at Paso de la Amada over 1,500 ballcourts have been excavated across Mesoamerica with the largest known court being at Chichén Itzá Courts were often placed in the center of the city close to other religious sites Recently, a ball court measuring 30-feet by 120-feet was completed in 2018 at the new Xochikalli community center in the Azcapotzalco neighborhood of Mexico City. The community center has gained attention from all over, including NPR We highly recommend watching the BBC Reel produced by Souleyman Messalti to watch the game being played by the association president Copyright © University of Wisconsin-Milwaukee and University of Wisconsin Board of Regents. The basins of interest span from the Pelotas of southern Brazil and Uruguay through the Punta del Este and into North Argentina with relatively few exploration wells drilled in its extensive >500,000 km2 area a number of international companies – including supermajors – have now established acreage positions and with work programme commitments estimated to be well in excess of $500m the area is going to see significant activity over the coming years The recent discoveries offshore Namibia have been made in Apto-Albian basin floor fans and Upper Cretaceous turbidites charged by a Lower Cretaceous Aptian source rock Although there are differences between the Namibian and South American passive margins with a noticeably thicker Tertiary section on the South American conjugate the same Aptian-aged source interval that is present in the Namibian Orange Basin can be seismically correlated across to South America With Cenomanian-Turonian source rocks also potentially developed and mature and the identification on seismic of similar reservoirs to those encountered in the Venus and Graff discoveries it is not surprising there has been so much recent interest 44 Blocks were awarded in the Pelotas Basin in the 4th Cycle of Permanent Offers The companies receiving awards included Petrobras and work programme commitments close to $400m significant activity can clearly be expected there The entire available offshore acreage in Uruguay has now been licenced and YPF entering the country in 2022 and Challenger Energy most recently confirming award of the final licence Work commitments for the next 3 to 4 years include the acquisition of 2,500 km2 of 3D seismic (Area OFF-4 Apache/Shell) and the drilling of an exploration well (Area OFF-6 Chevron have also entered offshore Uruguay again after an absence of around 50 years with a farm-in deal with Challenger on Block OFF-1 announced in March 2024 Chevron will fund the acquisition of 3D seismic in 2024/25 with the intention of drilling a well in 2027 With a further nod to the potential ties to Namibian success Charlestown Capital have taken a major investment in Challenger Energy Charlestown are a major shareholder in Sintana Energy already well-exposed to a number of exploration campaigns in the Namibian Orange Basin Moving further south along the continental margin and into Argentina Equinor and YPF are reported to be acquiring 3D seismic on Blocks CAN-102 with Shell and TotalEnergies also likely to be acquiring new seismic over their blocks this year the Equinor-operated Argerich-1 in Block CAN-100 This is targeting Cretaceous-aged reservoirs and as an initial test of the conjugate margin will no doubt be closely watched by operators and acreage-holders on both sides of the Atlantic The South Brazil-Uruguay-North Argentina conjugate margin to the Namibian offshore basins has very rapidly become a South American exploration hotspot With supermajors now holding large acreage positions extensive 3D seismic acquisition soon to get underway and at least 3 wells planned to be drilled by 2027 this is definitely a region to watch – and could well be where the next basin-opening discovery of global impact is made 05 Feb 2025 23:00:00 GMT?.css-1txiau5-AnswerContainer{color:var(--GlobalColorScheme-Text-secondaryText2);}Internacional won 3–0 over Brasil de Pelotas on Wed Predicted lineups are available for the match a few days in advance while the actual lineup will be available about an hour ahead of the match The current head to head record for the teams are Internacional 4 win(s) Have scored 3 goals in their last 5 matches 05 Feb 2025 23:00:00 GMT?Internacional won 3–0 over Brasil de Pelotas on Wed 05 Feb 2025 23:00:00 GMT.InsightsHave scored 7 goals in their last 5 matches Internacional is playing home against Brasil de Pelotas at Estádio José Pinheiro Borda on Wed Our editors will review what you’ve submitted and determine whether to revise the article June 23, 2022I brought to life the Global Online Learning Exchange (GOLE) project in my graduate class in Applied Behavior Analysis in the Fall 2021 semester after anxiously waiting for a year due to the pandemic.  Associate Professor in Special Education and Inclusive Education at the Universidade Federal de Pelotas (UFPel) in Southeast Brazil we partnered with community special education teachers in both New York City and Northeast Brazil.  We had three main objectives for our graduate students’ GOLE project: engage in culturally responsive practices in applied behavior analysis and special education apply content knowledge and skills to the project and engage in cultural humility during collaboration The main objective of our GOLE project was for graduate students from St John’s and UFPel to engage in culturally responsive practices in special education and applied behavior analysis while working together with a community teacher to help address the support needs of students with disabilities in their classrooms.  Together the graduate students developed a classroom behavior support plan for a student with a disability who was identified by the classroom teacher and the student’s parents It was developed considering the cultural needs of each student The secondary goal of our GOLE project was for our graduate students to put into practice the skills and methods they were learning in the graduate class The students collaborated in four separate groups that were supervised by myself and Dr We aligned our syllabi to teach the same content and text so our students would be able to apply the same skills The graduate students and community teachers also brought their own experiences and knowledge which were valued in the learning exchange.  In addition to the idea of cultural responsivity we wanted to teach our students to engage in cultural humility when collaborating with others from different cultures and countries Cultural humility in applied behavior analysis is engaging in mutually beneficial relationships where no one is the expert and everyone is on the same level; there is no hierarchy (Wright self-reflection is critical to engaging in cultural humility.  Throughout the semester we had several meetings with the graduate students and the community teachers to develop the plan understand the differences in special education processes and school systems in both countries and understand how to best support the teacher and student with a disability the graduate students had to continuously reflect on their role and their actions in the groups in which they were participating and collaborating.  This virtual learning exchange was critical not only to develop culturally-responsive practices in our students but also to provide opportunities for our graduate students to engage on a global scale and learn new global perspectives.  Cultural Humility in the Practice of Applied Behavior Analysis. Behavior analysis in practice, 12(4) https://doi.org/10.1007/s40617-019-00343-8 St. John’s University does not discriminate on the basis of race, color, national origin, sex, actual or potential parental, family, or marital status, pregnancy and related conditions, disability, or age in its programs and activities. The Office of Equal Opportunity and Compliance, [email protected] has been designated to handle inquiries regarding the non-discrimination policies To see the full seismic lines please download our PDF for the print issue available through this link: GEO ExPro Vol. 10 No. 4 Potential for large oil plays is refocusing the attention of E&P companies in the Pelotas Basin The Pelotas Basin is an untapped hydrocarbon province comprising a 280,000 km2 passive margin located on the south-east coast of Brazil Up to 7,000m thickness of Cretaceous and Tertiary post-rift clastics have been deposited in the basin including both Paleocene and Albian source rocks These source rocks are actively generating hydrocarbons today and there are seismic indications of an active working petroleum system similar in character to those found in the Niger Delta in West Africa Recent high quality 2D seismic data has imaged multiple potential reservoirs source rocks and direct hydrocarbon indicators in the basin The new seismic data show that since the inception of rifting between South America and Africa (at 125 Ma) the mouths of paleo tributaries of the Rio de la Plata moved along the margin several times The most recent depocenter comprises a four kilometer thick Tertiary clastic sequence which displays a world-class direct hydrocarbon indicator in the form of a 40,000 km2 sheet of Bottom Simulating Reflectors (BSR) which are confined inside the 3,000m Tertiary isopach and gas and condensate from this source rock subsequently migrated up through the prism via abundant gas chimneys and plumes to be trapped by the gas-water crystal phase change creating the BSR numerous oil seeps detected by Synthetic Aperture Radar and standard satellite imagery in the southern Pelotas Basin appear to define a linear feature approximately coincident with the 500–800m isobaths generated at depth below the Tertiary prism is migrating up to the impermeable solid base of the hydrate until it reaches the up-dip extent of the BSR (ca This oil is also trapped by stratigraphy or structure below the BSR creating a shallow oil play within the Tertiary prism itself Preliminary AVO analysis indicates large accumulations of free hydrocarbons are trapped in porous intervals below the solid hydrate layer Despite the abundant gas in these sequences it is the potential for large oil plays which is refocusing the attention of E&P companies in the Pelotas Basin The BSR represents the base of a solid layer of gas hydrates comprising water molecules that form cages containing methane (CH4) ethane (C2H6) and propane (C3H8) molecules trapped by high pressure The pressure and temperature conditions necessary for the formation of hydrate crystals require an environment with water depths greater than 300m The associated gas hydrates exist from approximately 100m beneath the sea floor to as deep as 450m beneath the sea floor The conjugate margin rift basins of West Africa and Brazil initially formed by east-west extension initiated during the early Cretaceous as the South American plate began to separate and rotate clockwise from Africa When the margins are reconstructed to their respective positions in the Albian it is informative to compare the respective margins for differences and similarities A reconstruction with today’s sediment thickness shows the asymmetry from the Pelotas Basin to the conjugate Namibe Basin This occurs because of large differences in the elevation of the terrains supplying a sediment source to each basin and it is possible to correlate an Apto-Albian sequence from Namibia to Pelotas In mini-basins directly above the break-up unconformity planar-bedded high amplitude Apto-Albian marine shales are correlated with units in the Namibe conjugate basin In Namibia these shales are considered the primary deepwater oil source for the light oil recovered from Wingat-1 Additional source intervals in the Pelotas Basin include syn-rift Cretaceous lacustrine shales (Kudu Cenomanian Turonian and Paleocene marine shales Both the Pelotas and Namibian Basins are underlain by thickened high-density volcanic crust with an abundance of seaward dipping reflectors Basin subsidence is controlled both by long-term post-rift thermal contraction and differential sediment loading and accommodation The new seismic data in the area illustrates the Pelotas Basin received substantially more sediment than the Namibe Basin and consequently the basement is deeper the prograding Tertiary wedge over the Paleocene source rock triggered a glide-plane detachment within the source rock Structural traps are developed within this gravity-driven structural complex along listric normal faults up-dip The Orange River Basin in Namibia exhibits a similar feature with a deeper detachment on the Cenomanian-Turonian source rock Although few wells have penetrated the deepwater section of either margin the interpretation of the new seismic data suggests deltaic processes generated numerous potential plays The supply of sediments via shelf and delta to the post-rift thermally subsiding margin was subject to both global sea level change and locally controlled delta lobe switching The new seismic data in the area indicates much of the potential reservoir was deposited by mechanisms common to many passive margin mixed-source deltaic systems Thin highstand sands are interpreted from pro grading clastic wedges in addition to aggradational shelf edge sequences and lowstand slope fan sands it appears the pro-delta slope was too steep for deposition of slope fans as these appear to have bypassed the slope and accumulated as thick ponded turbidites on the basin floor creating a very exciting play above Albian source rock Additionally through the Upper Cretaceous and Tertiary overbuilt prograding units appear to have suffered shelf collapse and slope failure in the northern parts of the basin depositing thick stacked slump sequences on the basin slope and floor Of the twelve wells drilled in the offshore Pelotas Basin to date three were effectively stratigraphic probes seven were drilled on the shelf and two wells tested Tertiary prospects in deeper water A more detailed examination of the seismic data indicates the deepwater Pelotas Basin contains substantially more prospective targets than the shelf There is considerable evidence of a working petroleum system with a Paleocene source rock presently within the oil window and correlation with the conjugate margin provides evidence of source rock in the Albian section which opens up a second deepwater oil play There are many good reasons to believe that the deepwater Pelotas Basin would make an excellent choice for blocks to be nominated in Brazil’s upcoming Bid Round 13 an International Organization of Pontifical Right aimed at creating and promoting the culture of encounter has announced the US launch of the Pelota de Trapo Program in Los Angeles The Program is named for the humble rag balls used by poor children around the world for their soccer games Pope Francis himself played football with a pelota de trapo as a young boy; later seeing children playing with the makeshift balls inspired Pope Francis to develop the Program internationally Scholas noted that the United States now joins Argentina Los Angeles was personally chosen by Pope Francis as the first US city to take part in the program The Holy Father noted that Los Angeles “will become a kind of worldwide capital of sport” by hosting several major sporting events over the next few years The Program will be undertaken in collaboration with the LA84 Foundation which supports youth sport and public education about the role of sports in positive youth development “Pope Francis advocates for unity and inclusion and his Pelota de Trapo Program will use the power of sports to inspire young people in our communities to live like we play.” “Our mission is to ensure all kids have opportunities for inclusion and access to the lifelong benefits that sports and play provide." The County and City of Los Angeles are also supporting the Program “Los Angeles County excitedly welcomes Scholas and the Pelota de Trapo education initiative to our region to cultivate leadership and opportunities for encounter for young people through a love of sports and play.” the Pelota de Trapo Program aims at “engaging a minimum of 30 schools annually establishing a virtual platform for increased engagement and producing a volunteer program for collaboration with families youth sports teams and partner organizations for support and increased growth.” Thank you for reading our article. You can keep up-to-date by subscribing to our daily newsletter. Just click here Following on from its recent collaboration with Pop Trading Company, Camper is celebrating the 25th anniversary of the Pelotas Ariel silhouette one of the brand’s most popular styles that made its debut in 1995 the shoe is set to re-release along with a lifetime guarantee to ensure its quality The silhouette is constructed from premium leather which has been stitched to the original 87-ball rubber outsole Camper has revealed a new line up of colorways covering the classic brown and black and more colorful options in red and blue Metrics details is a small G protein implicated in mRNA quality control It interacts with a translation-release factor 1-like protein Dom34/Pelota to direct decay of mRNAs with ribosomal stalls Although both proteins are evolutionarily conserved in eukaryotes the biological function of Hbs1 in multicellular organisms is yet to be characterized pelota is essential for the progression through meiosis during spermatogenesis and germline stem cell maintenance Here we show that homozygous Hbs1 mutant flies are viable which is due to defects in meiosis and spermatid individualization phenotypes that are also observed in pelota hypomorphic mutants Hbs1 mutants have no obvious defects in germline stem cell maintenance We show that Hbs1 genetically interacts with pelota during spermatid individualization Pelota with a point mutation on the putative Hbs1-binding site cannot substitute the wild type protein for normal spermatogenesis These data suggest that Pelota forms a complex with Hbs1 to regulate multiple processes during spermatogenesis Our results reveal a specific requirement of Hbs1 in male gametogenesis in Drosophila and indicate an essential role for the RNA surveillance complex Pelota-Hbs1 in spermatogenesis a function that could be conserved in mammals gene transcription is globally reduced and the transcripts used for late stages are stored in the cytoplasm until translation gene regulation at the translational level plays an important part to ensure proper sperm development the Dom34/Hbs1/Rli1 complex mediates the dissociation of the stalled ribosomes into 40 S and 60 S subunits the Pelo-Hbs1 complex regulates gene expression at translational level by facilitating the recycle of ribosomes and removal of aberrant mRNAs when elongation stalls occur the biological function of Hbs1 and Pelo-Hbs1 complex beyond female germline remains to be explored in multicellular organisms we investigated the biological function of Drosophila Hbs1 by generating and analyzing Hbs1 mutants We find that Hbs1 mutants are male sterile due to defects in meiosis and spermatid individualization during spermatogenesis We show that Pelo is also required for spermatid individualization in addition to its known role in early meiosis during spermatogenesis revealing that Pelo functions at multiple stages during male germline development we show that Hbs1 and Pelo genetically and physically interact with each other We thus demonstrate an essential role for the Pelo-Hbs1 complex in spermatogenesis (A) A scheme shows the genomic region of Hbs1 gene and the respective molecular lesions of the three alleles the entire coding region of Hbs1 was removed by imprecise excision of the P element EY09557 inserted before the transcriptional initiation site there were additional 420-bp and 15-bp DNA fragments (B) Quantitative RT-PCR analysis of the relative Hbs1 expression in the three Hbs1 mutants and genomic rescue flies Hbs1 expression could hardly be detected in Hbs11−/− testis and was significantly down-regulated in Hbs148−/− and Hbs1172−/− alleles The transgenic flies carrying the genomic region of Hbs1 could restore the expression of Hbs1 in mutant animals to the normal level arrow) of Hbs11 heterozygotes were full of sperm nuclei(arrow head) (D) No sperm was detected in Hbs11−/− mutant seminal vesicle and the sv was smaller than that of the Hbs11 heterozygote (E) The spermless phenotype could be fully rescued by a transgene carrying the genomic region of Hbs1 (F) A graph showing the fertility analysis of the Hbs1 mutants and genomic rescued flies The sterility could be rescued by the introduction of genomic Hbs1 samples were stained with the DNA dye DAPI (white) (D) The meiotic figure appeared normal in Hbs148−/− mutant cysts (E) Normal onion stage spermatids were observed in the Hbs1 genomic rescued testis (F) There was greater percentage of total irregular spermatids in the Hbs11−/− testes (G,H) The wildtype testis (G–G’) exhibited fully formed and well-focused spindle poles and the condensed chromosomes were either at the metaphase plate (arrowhead) or beginning to separate (arrow) the spindle microtubules failed to converge into the poles and were splayed outward and the chromosome could not be correctly separated (arrow).α-tubulin staining is in red Interestingly, in Hbs148 (Fig. 2D,F) or Hbs1172 homozygous mutant testes the abnormal germline cysts found in the null mutants were not observed Hbs148 and Hbs1172 homozygous testes contained no sperm in the seminal vesicles there were also some cysts with normal meiotic figures in Hbs11 testes yet no sperm was detected in the mutant seminal vesicles suggesting that Hbs1 is also required during later spermatogenesis Hbs1 is required for spermatid individualization (A) A schematic representation of four spermatids undergoing individualization: actin cones travel along the spermatid axonemes from the heads (the nuclear side) to the tips of the tails during the individualization The cytoplasmic content is removed and the syncytial membrane is remodeled during the movement of actin cones The syncytial spermatids (far left) are finally divided into individual sperm(far right) purple: elongated spermatids of different individualization stage (dark blue: spermatid nuclear bundle (C–E) Intact individualization complexes (ICs stained with phalloidin in red) were detected in wild type controls and 64 intact nuclei (revealed by DAPI staining in blue) were clustered together ICs moved from the basal end(C,D) towards apical end (C,E) ICs were formed but they were slightly disorganized (F,G) and they could not properly move away with disorganized actin cones scattering along the testicular tube (F,H) (I,J) ICs could not be detected in Hbs11−/− mutant testis and spermatid nuclei were randomly scattered along the testicular tubes (indicated by hollow arrowheads) (K) The number of ICs in Hbs148−/− and Hbs11−/− mutant testis was less than that in wild type control testes ICs number was restored in the flies with the genomic rescue of Hbs1 n = 17 for control and genomic rescue testes (L,N) Introduction of the genomic construct of Hbs1 in mutant flies rescued the nuclear bundling and subsequent ICs formation defect and ICs properly progressed away The ICs (actin cones) near the apical end are indicated by arrow heads We classified these alleles from weak to strong according to the severity of phenotypes and molecular lesions Genetic interaction between Pelo and Hbs1 during spermatogenesis (A) Removal of one copy of Hbs1 in wild-type background displayed no discernible phenotype on IC assembling (B) Scattered ICs and nuclei were frequently detected in testes genotyped UAS-peloRNAi/+; nosGal4/+ (C) Pelo-RNAi with one copy of Hbs1 removed led to an increase in severely disorganized nuclei and a dramatic inhibition in proper IC assembling(C) (D) Testes heterozygous for peloPB60 displayed normal spermatid and IC morphology (E) Hbs1 mutant testes with Hbs11 in trans to Hbs1172 displayed mild IC disorganization phenotype (F) Loss of one copy of pelo in Hbs11/Hbs1172 trans-heterozygotes led to decreased number of ICs and more disorganized nuclei and ICs Comparisons of IC number per testis among different genetic background are summarized in (G) (the normal looking ICs were quantified including the ICs that had progressed down in apical end.) (H) Schematic representation of the degree of the nuclear and IC defects in these flies IC disorganization and nuclei scattering phenotype was more severe in the peloPB60/+; Hbs11/Hbs1172 and UAS-peloRNAi/+; nosGal4/+; Hbs11/+ flies and the nuclear bundles These observations indicate that pelo and Hbs1 genetically interact with each other to promote spermatid individualization during spermatogenesis where those two proteins were co-localized in the cytoplasm (C: merged image with DAPI in blue) green) were both expressed in the cytoplasm of testicular germ cells when they were driven by nosGal4 Note that they could co-localize with each other in the germline cells (F) (G) Pelo interacted with Hbs1 in a yeast two hybrid assay Scale bar represents 10 μm in A–C and 15 μm in D–F The putative Hbs1-binding motif of Pelo is important for its function The effect of germline expression of different transgenes on rescuing spermatid defects in pelo1/PB60 testis (A,A’) The mutant control testis without transgene expression (C,C’) Transgene expression of Pelo (P210A) ICs were visualized by phalloidin staining (in green) and spermatid nuclei were recognized by DAPI (in red) (D,E) Quantitative analysis: transgene expression of wild type pelo could fully rescue the IC loss (E) and male sterility (D) caused by pelo mutant phenotype These data together suggest that Hbs1 is not required for the maintenance of GSCs implying that the stem cell function of Pelo is independent of Hbs1 Here we demonstrate a novel and specific function of Hbs1 in spermatogenesis in Drosophila Loss of function of Hbs1 causes defects in meiotic cell division and spermatid differentiation during spermatogenesis leading to male infertility with no apparent defect in viability and this revealed a male-specific germline function of Hbs1 collectively support that the physical interaction between Hbs1 and Pelo are evolutionary conserved from yeast to mammals it is therefore possible that Pelo-Hbs1 are important for ribosome recycling to ensure proper spermatogenesis It is possible that there are other redundant G proteins that can functionally substitute Hbs1 or interact with Pelo at different stages together with Hbs1 during spermatogenesis and Pelo may be a key player in the complex while Hbs1 may only have a supportive role It remains possible that the perdurance of gene product follow gene knock-out in stem cells may be sufficient to allow later spermatogenesis Therefore it would be worthwhile to re-evaluate the potential function of Pelo-Hbs1 complex in mammalian gametogenesis by germline depletion of Hbs1 peloPB60 was caused by a 107 bp deletion in the second exon and the predicted protein product is a peptide with 77 amino acids (compared to the full length with 395 amino acids) Flies homozygous for peloPB60 showed similar GSC loss phenotype during oogenesis and meiosis defect during spermatogenesis to transheterozyotes (peloPB60 in trans to a deficiency Df(2 L)Exel6024 in which the entire pelo locus is deleted) peloPB60 homozygous testes also displayed similar severity of meiosis defects to that of pelo1 homozygous mutants suggesting that both peloPB60 and pelo1 alleles can be considered as strong loss of function or genetic null alleles peloPA13 was resulted from a 557 bp insertion left by the original KG6646 P element and homozygous mutant cysts from testes squash displayed meiosis arrest either before the first or the second meiotic cell division Based on the severity of defects in female GSC maintenance and male meiosis we considered peloPA13as a hypomorphic allele The peloKG6646 chromosome was brought together with a transposase source by crossing peloKG6646/Cyo flies to CyO PBac{w[+mC] = Delta2-3.Exel}2/amos[Tft] flies which carried transposase on the second chromosome (Bloomington stock number:8201) PBac{w[+mC] = Delta2-3.Exel}2 were crossed to those with CyO/Sco PBac{w[+mC] = Delta2-3.Exel}2 chromosome were selected to establish excised lines Hbs1 alleles were established in a similar way using Hbs1EY09557 To generate pelo carrying Pro210 to Ala (P210A) mutation pelo was cloned from Canton-S ovarian cDNA library and ligated into pEasy-blunt Simple vector (TransGen Biotech) Single amino acid substitution was generated by Easy Mutagenesis System (TransGen Biotech) according to the manufacturer’s instruction Full length pelo coding sequence was amplified by PCR with primers 5′-GGTACCATGAAGCTGCTGGGCAAATA-3′ and 5′-TCTAGACTAGTCGCTATCGCTATCTGCC-3′ The PCR fragment was restriction digested with KpnI and XbaI All the plasmid DNA was injected for transformation via standard procedures To generate Pelo-RFP and Hbs1-GFP constructs for expression in Drosophila S2 cells ORFs of Pelo and Hbs1 were cloned into pAWG and pAWR vectors using the Gateway kit Constructs were transfected into Drosophila S2 cells using the standard calcium phosphate transfection protocol and the expression pattern of Pelo-RFP and Hbs1-GFP was examined 4 days after the initial transfection Fertility assay was performed as previously described58 Each male was put together with two w1118 virgin females in one food vail in twenty duplicates The flies were raised at 25 °C and separated from each other after 5 days The offspring were examined within 2 weeks Newly enclosed males were collected and manually dissected for testes in Testis Buffer (183 mM KCl Testes were subsequently transferred onto a coverslip containing a 2-ul drop of Testis buffer squashed and examined by phase contrast using a Zeiss Axio microscope fixed in 4% paraformaldehyde for 10 min at room temperature and then washed with PBT (PBS containing 0.1% Triton X-100) For DAPI (49,6-diamidino-2-phenylindole) staining samples were incubated with 1 mg/mL DAPI in PBT for 6 min and the reaction was stopped by washing samples with PBT samples were incubated with rodamine-phalloidin (Invitrogen; 1:10) at 37 °C for 2 hours and subsequently washed with PBT twice samples were fixed first and washed three times with PBST After blocking with 5% normal goat serum in PBT primary and secondary antibodies were then added according to standard procedures Antibodies used in this study include anti-α-spectrin antibody (3A9 Developmental Studies of Hybridoma Bank (DSHB) Alexa568-conjugated goat anti-mouse and Alexa 488-conjugated goat anti-rabbit secondary antibodies (Molecular Probes Samples were then mounted in 70% glycerol in 1 × PBS Images were captured by either a Zeiss Imager Z1 equipped with an ApoTome system or a Zeiss Meta 510 confocal microscope All images were processed in Adobe Photoshop and Illustrator ORF of Pelota was cloned into NdeI and EcoRI sites of pGADT7 AD Vector The primers used were 5′ CCCATATGAAGCTGCTGGGCAAATACGT 3′ ORF of Hbs1 was cloned into Nde I and BamH I sites of pGBKT7 DNA-BD vector and primers were 5′ CCCATATGTCGCGGCACAGGATAGT 3′ and 5′ CCGGATCCTAGCGGATCTTGGTGACCATT 3′ The yeast two hybrid assay was based on the MATCHMAKER kit (Clontech) pGBKT7-p53 with the Gal4 DNA-BD fused with murine p53 and pGADT7-T with the Gal4 AD fused with SV40 large T-antigen were used as positive control No datasets were generated or analyzed in the current study A.) 71–147 (Cold Spring Harbor Laboratory Press Toward a molecular genetic analysis of spermatogenesis in Drosophila melanogaster: characterization of male-sterile mutants generated by single P element mutagenesis The pelota locus encodes a protein required for meiotic cell division: an analysis of G2/M arrest in Drosophila spermatogenesis Yeast dom34 mutants are defective in multiple developmental pathways and exhibit decreased levels of polyribosomes The translation machinery and 70 kd heat shock protein cooperate in protein synthesis Novel G-protein complex whose requirement is linked to the translational status of the cell Endonucleolytic cleavage of eukaryotic mRNAs with stalls in translation elongation Analysis of Dom34 and its function in no-go decay Dom34 rescues ribosomes in 3′ untranslated regions Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay Conserved functions of human Pelota in mRNA quality control of nonstop mRNA Hbs1 and ABCE1 of mammalian vacant 80S ribosomes and stalled elongation complexes Nonstop-mRNA decay machinery is involved in the clearance of mRNA 5′-fragments produced by RNAi and NMD in Drosophila melanogaster cells Biochemical and biophysical research communications 484 Structure of yeast Dom34: a protein related to translation termination factor Erf1 and involved in No-Go decay Structural and functional insights into Dom34 The crystal structure of human eukaryotic release factor eRF1–mechanism of stop codon recognition and peptidyl-tRNA hydrolysis Kinetic analysis of interaction of eukaryotic release factor 3 with guanine nucleotides Dissection of Dom34-Hbs1 reveals independent functions in two RNA quality control pathways Structure of the Dom34-Hbs1 complex and implications for no-go decay Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution Kinetic analysis reveals the ordered coupling of translation termination and ribosome recycling in yeast Proceedings of the National Academy of Sciences 108 Structural basis for mRNA surveillance by archaeal Pelota and GTP-bound EF1alpha complex Omnipotent role of archaeal elongation factor 1 alpha (EF1alpha in translational elongation and termination Structural basis of highly conserved ribosome recycling in eukaryotes and archaea Rli1/ABCE1 Recycles Terminating Ribosomes and Controls Translation Reinitiation in 3′UTRs In Vivo Disruption of the pelota gene causes early embryonic lethality and defects in cell cycle progression Pelota mediates gonocyte maturation and maintenance of spermatogonial stem cells in mouse testes Pelota controls self-renewal of germline stem cells by repressing a Bam-independent differentiation pathway The RNA surveillance complex Pelo-Hbs1 is required for transposon silencing in the Drosophila germline Relationship between chromosome content and nuclear diameter in early spermatids of Drosophila melanogaster Mutations in the Essential Spindle Checkpoint Gene bub1 Cause Chromosome Missegregation and Fail to Block Apoptosis in Drosophila The Drosophila Protein Asp Is Involved in Microtubule Organization during Spindle Formation and Cytokinesis Visualizing the spindle checkpoint in Drosophila spermatocytes A requirement for the Abnormal Spindle protein to organise microtubules of the central spindle for cytokinesis in Drosophila Genetic dissection of sperm individualization in Drosophila melanogaster Dynamics of spermiogenesis in Drosophila melanogaster Regulation of zygotic gene expression in Drosophila primordial germ cells The product of the mammalian orthologue of the Saccharomyces cerevisiae HBS1 gene is phylogenetically related to eukaryotic release factor 3 (eRF3) but does not carry eRF3-like activity Function of the ski4p (Csl4p) and Ski7p proteins in 3′-to-5′ degradation of mRNA Recent structural studies on Dom34/aPelota and Hbs1/aEF1alpha: important factors for solving general problems of ribosomal stall in translation Ribosomal Stalling During Translation: Providing Substrates for Ribosome-Associated Protein Quality Control Annual review of cell and developmental biology 33 A convergence of rRNA and mRNA quality control pathways revealed by mechanistic analysis of nonfunctional rRNA decay Post-meiotic transcription in Drosophila testes Translational repression by MSY4 inhibits spermatid differentiation in mice don juan and don juan like encode proteins of the spermatid nucleus and the flagellum and both are regulated at the transcriptional level by the TAF II80 cannonball while translational repression is achieved by distinct elements A conserved element in the leader mediates post-meiotic translation as well as cytoplasmic polyadenylation of a Drosophila spermatocyte mRNA A new translational repression element and unusual transcriptional control regulate expression of don juan during Drosophila spermatogenesis Post-transcriptional regulation of the meiotic Cdc25 protein Twine by the Dazl orthologue Boule Translational control of meiotic cell cycle progression and spermatid differentiation in male germ cells by a novel eIF4G homolog couples translational control to meiosis and differentiation in Drosophila spermatocytes Loss-of-function analysis reveals distinct requirements of the translation initiation factors eIF4E eIF4G and eIF4G2 in Drosophila spermatogenesis A discrete transcriptional silencer in the bam gene determines asymmetric division of the Drosophila germline stem cell The highly conserved LAMMER/CLK2 protein kinases prevent germ cell overproliferation in Drosophila A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method The CPEB Protein Orb2 Has Multiple Functions during Spermatogenesis in Drosophila melanogaster Download references Vienna Drosophila RNAi Center and Developmental Studies Hybridoma Bank for fly stocks and reagents This work was supported by grants from the Chinese Ministry of Science and Technology (2017YFA0103602 is supported by a National Youth Fund (31601059) from National Nature Science Foundation of China Present address: Genomics Institute of the Novartis Research Foundation Tsinghua Institute of Multidisciplinary Biomedical Research performed data analysis and wrote the paper Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41598-019-39530-6 Sign up for the Nature Briefing newsletter — what matters in science