Our editors will review what you’ve submitted and determine whether to revise the article Dschang, town located in northwestern Cameroon. It is situated on a forested plateau northwest of Yaoundé Volume 3 - 2024 | https://doi.org/10.3389/fpara.2024.1509261 Background: Resistance to antimalarial drugs remains a major obstacle to malaria elimination targeted amplicon sequencing is being adopted for surveilling resistance and dissecting the genetics of complex malaria infections genotyping of parasites and detection of molecular markers drug resistance in resource-limited regions requires open-source protocols for processing samples and rapid methods for processing numerous samples including pooled sequencing Methods: Plasmodium falciparum Streamlined Multiplex Antimalarial Resistance and Relatedness Testing (Pf-SMARRT) is a PCR-based amplicon panel consisting of 15 amplicons targeting antimalarial resistance mutations and 9 amplicons targeting hypervariable regions This assay uses oligonucleotide primers in two pools and a non-proprietary library and barcoding approach Results: We evaluated Pf-SMARRT using control mocked dried blood spots (DBS) at varying levels of parasitemia and a mixture of 3D7 and Dd2 strains at known frequencies showing the ability to genotype at low parasite density and recall within-sample allele frequencies We then piloted Pf-SMARRT to genotype 100 parasite isolates collected from uncomplicated malaria cases at three health facilities in Dschang Antimalarial resistance genotyping showed high levels of sulfadoxine-pyrimethamine resistance mutations including 31% prevalence of the DHPS A613S mutation No K13 candidate or validated artemisinin partial resistance mutations were detected but one low-level non-synonymous change was observed used to assess complexity of infections and parasite diversity and relatedness showed similar levels and patterns compared to molecular inversion probe (MIP) sequencing While there was strong concordance of antimalarial resistance mutations between individual samples and pools low-frequency variants in the pooled samples were often missed Pf-SMARRT is a robust tool for assessing parasite relatedness and antimalarial drug resistance markers from both individual and pooled samples Control samples support that accurate genotyping as low as 1 parasite per microliter is routinely possible Multiplex targeted deep sequencing can also help to understand how parasites are related to one another By targeting single nucleotide polymorphisms (SNPs) or microhaplotypes multiplex assays can be used to study overall population diversity or even to track transmission by leveraging metrics like identity-by-descent (IBD) an assay must be highly sensitive in genotyping low-parasitemia samples and easily implemented in resource-limited areas One way to achieve these goals is to design approaches for which materials are easily acquired through multiple commercial sources and detailed protocols are publicly available Recent reports (published 220-2024) of molecular markers of P falciparum antimalarial resistance mutations in Cameroon We also evaluated the performance of the assay in detecting and quantifying parasite genetic variation using pooled samples By leveraging previously published and validated primer pairs (Protocol 1 in Supplementary Data Sheet 1) as well as development of specific primers for the assay we targeted 15 antimalarial resistance polymorphisms as well as 9 regions of hypervariability that don't contain any length polymorphisms This resulted in a panel containing 24 target amplicons The major antimalarial resistance polymorphisms targeted by the assay include: 575-623 and 668-702; covering almost all current validated (F446I V568G) artemisinin partial resistance mutations ● CRT: amino acids 43-90; including K76T ● DHFR: amino acids 41-81 and 97-123; including Samples were sequenced with at least 2 replicates allowing for improved de-noising SeekDeep can examine each replicate individually limiting erroneous haplotypes with depth and frequency filters Replicates can be combined into a final sample call which offers the same depth and frequency filters but requires haplotypes to be in both replicates thereby minimizing false haplotypes and more accurately estimating within-sample haplotype frequency DNA from the mocked blood spots at varying levels of parasitemia (parasites per microliter: p/µL) (10,000 p/µL and 1 p/µL) was amplified and sequenced twelve times using two replicates per sample a mixture containing 88% 3D7 and 12% Dd2 genomic DNA (MRA-102G and MRA-150G VA) at a concentration of 1,000 p/µL was sequenced twelve times with two replicates per sample Final SeekDeep sample filtering was: 1) a minimum of 100 reads per sample; 2) a minimum of 100 reads per replicate; 3) amplicons were restricted to the exact expected length; 4) a minimum frequency within a sample for a haplotype of 1%; and 5) a requirement that haplotypes needed to pass thresholds in both replicates to be counted informed consent was obtained from a parent or guardian and assent from adolescents Molecular analysis of de-identified samples and data were deemed non-human subjects research by the University of North Carolina IRB To create a standard curve and determine estimated parasitemia for each sample mocked DBS containing whole blood spiked with known densities of cultured 3D7 P VA) were amplified alongside clinical samples The resulting Ct values of the standard dilutions were plotted against known parasite densities to form a standard curve then each sample’s Ct was plotted on the standard curve to calculate each sample’s parasitemia All runs included non-template control reactions Samples with low estimated parasitemia on initial real-time PCR runs were checked for DNA concentration using a Qubit fluorometer (Invitrogen California) and Qubit 1X HS dsDNA quantification reagent to ensure proper extraction occurred The workflow of clinical samples is shown in Figure 1 The 100 samples were sequenced with two replicates To evaluate initial amplification and determine if additional sequencing was needed we assessed each replicate separately using less stringent filtering criteria These filters were set to be as permissive as possible and required only a minimum of 25 reads per replicate samples were either: 1) considered complete (if the same haplotypes were found in both replicates and the difference in haplotype frequency between replicates was never greater than 10%); or 2) were re-amplified and resequenced with two new replicates (different haplotypes detected between replicates and/or >10% variation in haplotype frequency found between replicates for any haplotype) The replicate PCRs of one sample still showed an increase in the number of haplotypes with >10% variation in haplotype frequency upon resequencing and the original replicates for that sample were retained After repeat sequencing for samples requiring new replicates the final data was called at the sample level using the same filters applied to the controls One hundred samples from parasitemic participants were used in the study These samples were PCRed and sequenced with replicates (R1 and R2) and processed in SeekDeep we examined the replicates for each field sample Replicates that differed in the haplotypes reported or that showed >10% variation in haplotype frequency between replicates (N=35) were re-amplified and re-sequenced a subset of 50 samples also underwent MIP sequencing to allow for comparison of relatedness and antimalarial resistance frequency the 100 samples were used to generate non-overlapping pools of N=10 and N=100 to evaluate the utility of the assays for pooled sequencing All final sample (and pool) genotyping calls are based upon haplotypes that occurred in both replicates (R1 and R2) and within sample haplotype frequency was averaged between replicates A subset of 50 isolates were selected for MIP sequencing. Samples were captured and sequenced as previously described using capture probes for genome-wide single nucleotide polymorphisms and probes targeting known antimalarial resistance polymorphisms (Verity et al., 2020). Data was processed using MIP analyzer as previously described (Verity et al., 2020) A variant required at least 3 unique molecular identifiers to be called and a sample required a depth of 10 UMIs Pools of samples were generated with a range of pool sizes The number of pools available at each sample size was a function of the available number of nonoverlapping groups of samples Each pool was sequenced in triplicate with two replicates informing each pool The pools were analyzed in SeekDeep with the same clustering parameters as the individual samples except that in the pooled samples the minimum frequency within a sample for a haplotype was set to 0.5% on the assumption that more samples should give more power to confidently call low frequency haplotypes p-value comparisons across groups for categorical variables are based on the chi-square test of homogeneity whereas p-values for continuous variables are based on the Kruskal-Wallis test for median The program was run in the categorical method format: heterozygous call as 0.5 homozygous alternative allele as 1 and no call as −1 the probability that two amplicon haplotypes will be different was determined as 1-(p12+p22….+pn2) are the population frequencies of each haplotype and n is the total number of haplotypes in the population in that any of the amplicons would have different haplotypes was estimated as 1-((1-He1)*(1-He2)*…(1-Hei)) where He1 are the heterozygosities at each amplicon and i is the total number of amplicons To assess whether parasite populations within Cameroon clustered by their geographic origin we conducted principal component analysis (PCA) using SNPRelate function in R 4.2.1 software The results were visualized using ggplot2 or GraphPad Prism (v.10.2.3) Upon testing the 3D7/Dd2 mixture (ratio 88:12), we observed high concordance between measured and expected allele frequency for the diversity markers and antimalarial SNPs (Supplementary Figure S7; Supplementary Table S2) with one exception The DHPS 436/437 amplicon consistently underestimated the Dd2 SNP frequency when detected (mean 1.09% These controls were run separately from the clinical samples which routinely detected multiple haplotypes in the clinical samples it is unclear why this amplicon failed in the control reactions MDR1 SNP frequencies varied from expected due to the copy number variation in Dd2 which contains different point mutations in each copy There was no single amplicon that was the most reliable indicator of maximum haplotype count being associated with the maximum haplotype count in 50 samples while Heome-D was associated with the maximum haplotype count in 46 samples Estimated complexity of infection for each sample Red text denotes the mean (as a central dot) and standard deviation (as arms above and below the mean) Black dots represent COI values for individual samples (A) Depicts the COI for Pf-SMARRT based on the amplicon that provided the highest number of haplotypes (B) Shows the estimated COI determined by THE REAL McCOIL for Pf-SMARRT and MIPs Antimalarial resistance infection genotypes comparing Pf-SMARRT and MIPs Prevalence of antimalarial resistance polymorphisms by Pf-SMARRT vs MIP MIPs provided visually more clustered samples than Pf-SMARRT Principal component analysis of 100 Cameroonian samples collected from three hospitals in Dschang Samples are color coded by the patient's village/town/city of origin Diversity markers will have variable information for discerning strains in different populations. In Cameroon, these diversity markers had a range of heterozygosity from 0.32 to 0.95 (Table 4) AMA1 showed the greatest within-population heterozygosity There were between 5 and 37 haplotypes in the population depending on the loci Diversity of amplicon microhaplotypes in the population when a mutation was between 20% and 40% only 22/27 (81.5%) were detected and for polymorphisms less than 20% only 50/79 (63.3%) were detected Correlation of antimalarial resistance SNP frequency in pools compared to individual samples Weighted mean allele frequency for key resistance mutations was estimated across replicates for each pool group and compared with allele frequencies across replicates for the individual samples included in each pool Allele frequencies in the pool were weighted by parasite density of each individual sample included in the pool Pearson’s correlation values were high across all pool groups where ## is the pool size and # is the pool number We report the development and validation of a highly multiplexed amplicon deep sequencing tool Pf-SMARRT and successful application to field samples from Cameroon Our goal was to develop a simple assay that was freely available using standard reagents to characterize key antimalarial drug resistance genes and provide basic information on parasite COI The assay leverages no custom products and the necessary protocols are available within this publication Pf-SMARRT provided robust data across a range of parasitemias with most amplicons successfully genotyped at 1 p/µL The assay was then used to characterize antimalarial resistance profiles in Dschang with most samples genotyped at all amplicons successfully The assay was compared to MIPs in a subset of samples and showed an overall similar pattern of parasite population clustering based on PCA but demonstrated a higher rate of calling minor haplotypes resulting in higher prevalence for drug resistance molecular markers and higher COI estimates we evaluated the assay using pooled deep sequencing to characterize how well it would detect antimalarial resistance SNPs in sample pools of different sizes showing that increased pool size improves the accuracy of frequency calls but can result in missed low-frequency SNPs in the pool Pf-SMARRT has multiple advantageous characteristics which make it particularly useful for conducting MMS the DHFR A613S was found at a prevalence of 31.0% (CI: 21.6-40.1) When comparing Pf-SMARRT to MIPs, a higher number of mixed infections were found with Pf-SMARRT than with MIPs (Table 2). This resulted in an increased estimated prevalence for most markers (Table 3) Pf-SMARRT identified mixed genotypes in 12.5% (81/650) of loci whereas MIPs found mixed genotypes at 6.4% of loci (40/628) There are likely two reasons for this difference PCR amplicons may be more efficient than capture-based approaches like MIPs This is primarily due to the nature of PCR amplification ensuring that even minor clones within a sample are more likely to be captured and sequenced The inherent nature of PCR allows for amplification of low-abundance targets making it easier to detect mixed genotypes and rare variants The depth of sequencing is another critical aspect that enhances the performance of Pf-SMARRT Deeper sequencing of a smaller number of loci allows for a more finely articulated view of the genetic diversity within a sample making it possible to detect low-frequency alleles that may exist in mixed infections This increased depth is particularly valuable in malaria research where understanding the prevalence of minor variants can inform public health strategies and resistance monitoring While MIPs can achieve significant coverage they often operate at shallower sequencing depths (10X-50X coverage) This can lead to a loss of sensitivity in detecting low-density infections limiting their ability to uncover important genetic variants It is important to note that while MIPs can achieve similar sensitivity to Pf-SMARRT with increased coverage and depth the practical implementation of this is often constrained by factors such as cost and technical complexity Pf-SMARRT stands out as a more effective tool for detecting all clones and resistance mutations identified by MIPs while also capturing more minor clones and rare variants This enhanced sensitivity is vital in regions where malaria transmission declines and efforts shift toward pre-elimination strategies The ability to detect low-density infections and subtle genetic variations makes Pf-SMARRT particularly useful for MMS enabling researchers and public health officials to make informed decisions in the fight against malaria the ability to detect and determine the relative frequency of alleles occurring at moderate to high frequency was good especially in pools with higher numbers of samples the use of a pooling approach may be limited to monitoring known mutations after emergence Increasing throughput for individual sample sequencing by making the assay protocol straightforward and scalable is necessary for evaluating introduction or emergence of a mutation this version of the assay is missing coverage for several key drug resistance mutations including P574L in K13 the ligation based library preparation is more complicated and time consuming than a PCR-based library preparation Modifications to resolve these limitations are underway Despite sequencing in duplicate and using a relatively conservative minor frequency cut off of 1% we detected three false positive haplotypes in our control experiments although all false-positives occurred at the lowest parasitemia This represented only 3 of 288 loci genotyped in the 1 parasite/µL controls one amplicon (DHPS 437) failed to detect the Dd2 minor variant consistently It is unclear what happened during this experiment as no other indication of problems occurred with this amplicon in the other controls or clinical samples One of the diversity targets (Heome-H) was consistently under-sequenced relative to other amplicons but had sufficient coverage to allow calls in most samples (76/100) we did not directly test the assay in low-density infections from Cameroon (a single low density parasitemia of 0.5 p/µL successfully genotyped at 24/24 amplicons) thus we do not know how it will perform using such clinical samples although the mocked blood spots included whole blood spiked with culture grown parasites and Pf-SMARRT represents a valuable tool for conducting MMS providing detailed genotyping of 24 amplicons in an open source platform Control experiments using mocked blood spots and known mixtures of different strains provide support that the assay should accurately report within-sample frequency and reliably detect genotypes in low density infections The assay had a high success rate in samples with what should be RDT-detectable parasitemia from Dschang It proved sensitive for minority clones in infections and had a low false positivity rate which occurred only at very low parasitemia While pooled sequencing had overall good correlations to individual samples in terms of antimalarial resistance frequency the lowest frequency alleles were commonly missed in the pools This suggests that pools may not be appropriate for surveillance of rare alleles in a population but could help monitor the frequency of common alleles the insights provided by Pf-SMARRT will be useful for understanding and monitoring the transmission dynamics of P as more labs and control programs gain access to next generation sequencers in Africa the use of MMS to monitor malaria populations will only increase Pf-SMARRT represents a tool that uses easy to access materials and can easily be implemented across any Illumina platform to provide robust characterization of antimalarial resistance polymorphisms and parasite relatedness The original contributions presented in the study are publicly available. This data can be found here: NCBI SRA, accession PRJNA1171111 and https://github.com/bailey-lab/pfsmarrt_cameroon_7-6-24 The studies involving humans were approved by Cameroon Baptist Convention Health Board (FWA00002077) The studies were conducted in accordance with the local legislation and institutional requirements Written informed consent for participation in this study was provided by the participants’ legal guardians/next of kin The author(s) declare financial support was received for the research This project was funded by the National Institutes for Allergy and Infectious Diseases (R01AI156267 to JB and JJ This work was partially supported by the Bill & Melinda Gates Foundation (INV-050353 to JP) and the EDCTP2 career development fellowship (TMA2020CDF-3171 to IA) Under the grant conditions of the Foundation a Creative Commons Attribution 4.0 Generic License has already been assigned to the Author Accepted Manuscript version that might arise from this submission This work was also partially supported by the Yang Biomedical Scholars Fund from the University of North Carolina We thank the laboratory and clinical staff of the Dschang Regional Hospital Annex Vincent Catholic Hospital and the Catholic Hospital in Batsingla for their assistance in sample collection for this study The following reagent was obtained through BEI Resources NIH: Genomic DNA from Plasmodium falciparum JP reports research support from Gilead Sciences non-financial support from Abbott Laboratories The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest The author(s) declare that no Generative AI was used in the creation of this manuscript All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpara.2024.1509261/full#supplementary-material A snapshot of the prevalence of dihydropteroate synthase-431V mutation and other sulfadoxine-pyrimethamine resistance markers in Plasmodium falciparum isolates in Nigeria Arboviruses as an unappreciated cause of non-malarial acute febrile illness in the Dschang Health District of western Cameroon Aranda-Díaz A. 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This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) distribution or reproduction in other forums is permitted provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited in accordance with accepted academic practice distribution or reproduction is permitted which does not comply with these terms *Correspondence: Jonathan J. Juliano, amp1bGlhbm9AbWVkLnVuYy5lZHU= †These authors share first authorship ‡These authors share senior authorship Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher 94% of researchers rate our articles as excellent or goodLearn more about the work of our research integrity team to safeguard the quality of each article we publish image: This image illustrates the impact of Ruspolia nitidula (grasshopper) and Clupea harengus (fish) meals on rat health Rats fed with grasshopper meal showed optimal improvements in libido whereas those fed with fish meal exhibited moderate enhancements in similar health markers As the global population grows and traditional livestock production increasingly strains environmental resources there is a rising interest in alternative protein sources are abundant in regions like Cameroon and provide essential nutrients Addressing these challenges calls for in-depth studies on the nutritional benefits of insects such as Ruspolia nitidula Conducted by the University of Dschang, Cameroon, and published (DOI: 10.26599/FSAP.2024.9240068) in the journal Food Science of Animal Products on August 30 this study examined the effects of substituting traditional Clupea harengus fish meal with Ruspolia nitidula grasshopper meal in rat diets researchers evaluated how this dietary change impacted libido assessing the insect meal's potential as a viable alternative protein source The study demonstrated that replacing fish meal with Ruspolia nitidula grasshopper meal resulted in significant health improvements in rats Those on the grasshopper diet exhibited enhanced libido with increased intromissions and ejaculations compared to rats on fish meal or protein-deficient diets with 94.58% of hairs in optimal condition in the grasshopper-fed group compared to just 5.55% and 0.27% in the fish meal and protein-deficient groups the grasshopper-fed rats showed greater body weight gain indicating overall better health and nutrition These findings underscore the grasshopper meal's potential as a sustainable and nutritionally superior alternative protein source "Our findings highlight the significant potential of edible insects like Ruspolia nitidula as alternative protein sources The grasshopper meal not only meets nutritional needs but also offers substantial health benefits with far-reaching implications for both animal and human diets." This research emphasizes the potential of Ruspolia nitidula as a sustainable the findings suggest that grasshopper meal could play a role in addressing human malnutrition With ecological advantages and health benefits edible insects present a compelling solution for future food security and dietary enhancement The North Cameroon Association for Ecological and Food Transition (ABC-ECOLO) for funding this study About Food Science of Animal Products Food Science of Animal Products, sponsored by Beijing Academy of Food Sciences, published by Tsinghua University Press and exclusively available via SciOpen, is a peer-reviewed open access international journal that publishes the latest research findings in the field of animal-origin foods The research scope includes the quality and processing characteristics of food raw materials the relationships of nutritional components and bioactive substances with human health product flavor and sensory characteristics the control of harmful substances during processing or cooking storage and packaging; microorganisms and fermentation illegal drug residues and food safety detection; authenticity identification; cell-cultured meat About SciOpen  SciOpen is an open access resource of scientific and technical content published by Tsinghua University Press and its publishing partners SciOpen provides end-to-end services across manuscript submission and expert advice to ensure each journal’s development 10.26599/FSAP.2024.9240068 Nutritional and health benefits of Cameroonian grasshopper (Ruspolia nitidula) meal: effects on libido are not responsible for the accuracy of news releases posted to EurekAlert by contributing institutions or for the use of any information through the EurekAlert system Copyright © 2025 by the American Association for the Advancement of Science (AAAS) (Business in Cameroon) - Dschang cliff collapse halted traffic between West and Littoral regions CFA3 billion allocated in 2025 public investment budget for repairs Chinese company CFHEC to complete reconstruction in four months Cameroon’s Ministry of Public Works has set aside over CFA3 billion to rebuild the collapsed road along the Dschang cliff in the West region The project is listed in the 2025 public investment budget and will be carried out by China First Highway Engineering Corporation (CFHEC) Work is expected to last around four months killing at least five people and causing major property damage traffic between the West and Littoral regions has been cut off engineers recommended a new 367-meter route to bypass the collapsed section Although the final contract has not yet been signed Public Works Minister Emmanuel Nganou Djoumessi officially launched the project on March 15 CFHEC has started setting up at the site and moving in equipment An alternative route is currently being used to maintain limited traffic flow including clearing and grading the future road Kribi Bitumen Plant Set to Start Construction in 2025 with Government Backing CEMAC Bond Market Hits CFA 8.45 Trillion in March 2025, Interest Rates Drop Cameroon’s Timber Output Projected to Rise in 2025 Despite Higher Export Taxes Central Africa Stock Exchange Sees 98% Drop in Trading Value in Q1 2025 Every week the economy and investment news from Cameroon Mboa Paris Trains 30 Young Cameroonians to Boost Tech and Entrepreneurship Cameroon Audit Targets Former Officials for Mismanagement in Agricultural Project Camwater Seeks Global Bids to Launch Bottled Water Lines in Five Cities Bafoussam Workshop Highlights Benefits of Cameroon-EU Trade Agreement Cameroon Could Reach 350,100 Tons of Cotton in 2025 (Beac) Paul Biya Appoints Johnny Razack as Chair of Cameroon’s National Investment Company Cameroon Refuses Work Visa Renewal for Casino and Super U Boss Over Toxic Workplace Claims Cameroon Joins Global Charter to Fight Illegal Fishing Rescue workers have found 12 bodies after landslides buried a road in western Cameroon stating that there is no expectation of locating any survivors State television CRTV relayed the statement made by Augustine Awa Fonka “In our opinion there is no longer any possibility of finding survivors,” he told the station “Only 12 bodies had been recovered from the site of the disaster “Dozens more people are still missing and the search for bodies is still continuing” Two landslides occured at the Dschang cliff road on Tuesday the second struck as emergency workers used heavy machines to clear the road vehichles hit by the landslides include three 20-seater coaches five six-seater vehicles and several motorbikes According to the latest data from the World Health Organisation(WHO) Cameroon’s roads are notoriously dangerous with almost 3,000 deaths each year in accidents or more than ten deaths per 100,000 people a tractor-trailer carrying passengers plunged off a cliff road into a ravine near the town of Dschang killing eight people and injuring 62 others Chinomso Sunday is a Digital Content Writer at News Central contributing her combined skills to her professional endeavours a former close associate of Senator Rabiu Musa Kwankwaso and a notable… Home › Wellness By StudyFinds Staff Reviewed by Steve Fink Cameroon — Forget kale and quinoa – the next superfood might be crawling around in your backyard Researchers in Cameroon have uncovered the surprising health benefits of a grasshopper-based diet suggesting these insects could be the key to better sleep The study published in the journal Food Science of Animal Products reveals that rats fed a diet of Ruspolia nitidula a type of edible grasshopper common in Cameroon experienced significant improvements in several health markers compared to rats on other diets As the world grapples with food security issues and a growing population, scientists are increasingly turning to unconventional protein sources. Insects with their high nutritional value and low environmental impact led by researchers at the University of Dschang in Cameroon adds weight to the argument that insects could be more than just a sustainable food source – they might also offer unique health benefits compared the effects of three different diets on rats: one based on Ruspolia nitidula meal (made from ground-up grasshoppers) another using traditional fishmeal from herring and a third diet lacking in animal protein they monitored various health indicators in 216 rats The results were striking. Rats on the grasshopper diet showed marked improvements across several health measures. Perhaps most notably, these rats experienced a significant boost in libido, with an increased frequency of sexual behaviors compared to rats on other diets The grasshopper-fed rats also enjoyed better sleep gaining nearly 12 hours of extra sleep per week compared to rats on the protein-deficient diet The study found that an astonishing 94.58% of hairs examined from grasshopper-fed rats were in excellent condition – lying flat and flexible to the touch only 5.55% of hairs from fishmeal-fed rats and a mere 0.27% from protein-deficient rats showed similar quality with grasshopper-fed rats maintaining a healthy average weight of 180.10 grams slightly higher than the fishmeal group at 172.76 grams Rats on the protein-deficient diet lagged significantly behind at 146.15 grams “Our findings highlight the significant potential of edible insects like Ruspolia nitidula as alternative protein sources,” Abdoul says in statement “The grasshopper meal not only meets nutritional needs but also offers substantial health benefits with far-reaching implications for both animal and human diets.” While the idea of chomping on grasshoppers might not appeal to everyone this research opens up exciting possibilities for nutrition and health As we face global challenges in food production and seek ways to improve human health unconventional solutions like insect-based diets could play a crucial role The researchers divided 216 rats (108 males and 108 females) into three groups of 72 each Each group received a different diet: grasshopper meal the team measured various health indicators monitored sleep patterns using special sensors and observed sexual behaviors through video recordings They also tested urine and fecal pH to assess digestive health The grasshopper diet led to significant improvements across multiple health measures Rats on this diet showed increased sexual activity with more frequent mounting and intromission behaviors gaining about 12 extra hours of sleep per week compared to the protein-deficient group with over 94% of hairs examined showing ideal characteristics and digestive health indicators were more balanced It’s important to note that this study was conducted on rats and results may not directly translate to humans The study duration of 12 weeks provides insight into short-term effects the study focused on a single species of grasshopper and effects may vary with other insect species The researchers suggest that the unique nutritional profile of Ruspolia nitidula contributes to the observed health benefits They highlight the potential of insect-based diets as a sustainable and nutritious food source especially in regions where these insects are already part of traditional diets The study opens up new avenues for research into insect-based nutrition and its potential health applications from improving sexual health to addressing hair loss and sleep issues The study was funded by the North Cameroon Association for Ecological and Food Transition (ABC-ECOLO) The researchers declared no conflicts of interest It’s worth noting that while this funding source suggests local interest in sustainable food solutions readers should always consider potential biases in research funding StudyFinds sets out to find new research that speaks to mass audiences — without all the scientific jargon summarized versions of research that are intended to inform the reader as well as stir civil but always thoroughly reviewed and edited by a Study Finds staff member transparent research summaries that are intended to inform the reader as well as stir civil educated debate. We do not agree nor disagree with any of the studies we post we encourage our readers to debate the veracity of the findings themselves. All articles published on StudyFinds are vetted by our editors prior to publication and include links back to the source or corresponding journal article become bald and remember the good sex from the past and dismiss the future of such fun ©2025 Study Finds. 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Privacy Policy • Disclosure Policy • Do Not Sell My Personal Information Unveiling Tomorrow's Cameroon Through Today's News that disrupted traffic along the Dschang-Santchou route the governor confirmed that the second landslide resulted in four fatalities and three heavy machines that were clearing the initial landslide “Rescue operations are ongoing to recover the bodies of other potential victims,” said Governor Augustine Awa Fonka The Dschang cliffside has long been recognized as a high-risk zone is one of the first to result in such severe casualties and damage Dschang cliff, an area prone to landslides and the site of many fatal accidents This landslide buried a section of the regional road leading to the deaths of over 12 people and disrupting traffic between Dschang and Santchou has announced the reconstruction of the road The information in contained in a release issued on 18th February According to the release a new design will be incorporated which includes a drainage system and a hydraulic structure which will manage rainwater and prevent future landslides travelers are recommended toq continue using the Molong-Kekem-Bafang-Bandja-Bandjoun section of National Road No The minister has urged residents to help preserve hydraulic structures he equally assured the public that the selected company’s expertise will confirm quality work full reconstruction will commence after the validation of execution studies conducted by the company in charge restoring safe and faster travel between Dschang and Santchou les actualités camerounaises et la revue de presse Lebledparle.com à ne pas manquer Δdocument.getElementById("ak_js_1").setAttribute("value",(new Date()).getTime()) Actualité du Cameroun ce matinDschang cliff Lebledparle.com is a site dedicated to news in Cameroon news from Cameroon and all useful information analyzes and all the news in real time and continuously Two landslides claimed the lives of at least four people and trapped others in their cars in western Cameroon on Tuesday according to a statement from the local governor “The provisional death toll stands at four” and “searches are continuing on the ground to find the bodies of other victims of this natural disaster” said West regional governor Augustine Awa Fonka Awa Fonka reported that the first landslide occurred around mid-morning on the Dschang cliff road a section known for accidents that run through a mountainous region of the central African country The governor said this incident briefly disrupted traffic and emergency responders used heavy construction equipment to clear the road A second “large-scale” landslide that occurred during the work buried “three heavy machines involved in the clearing” Authorities halted traffic and advised local residents to avoid the area Cameroon’s highways are known to be dangerous with nearly 3,000 fatalities reported annually due to accidents, equating to over ten deaths for every 100,000 residents, based on the most recent data from the World Health Organisation a tractor-trailer filled with passengers fell off a cliff road into a ravine close to the town of Dschang resulting in the deaths of eight people and injuring 62 others Geology and geography experts from the University of Dschang have issued a warning of a possible third landslide following two deadly collapses on November 5 These landslides have already claimed five lives academic researchers conducted a field study to assess the area Their analysis suggests that the two previous landslides were part of a large-scale collapse in two phases they have cautioned that a third landslide could still occur the experts have recommended expanding the safety perimeter to keep residents away and prevent further casualties The geography team emphasized the need to protect the local population by securing the area and implementing a sustainable management plan to reduce the risk of similar incidents in the future researchers are urging authorities to restrict public access and temporarily ban traffic along the route These recommendations align with the government’s response as Western Regional Governor Awa Fonka Augustine has enforced a temporary closure of the Dschang-Santchou road and restricted access to the danger zone Minister of Transport Emmanuel Nganou Djoumessi has announced the identification of alternative routes declaring that the Dschang-Santchou road is no longer viable These actions aim to safeguard lives and prevent future tragedies in this increasingly unstable area Rescue workers have recovered 12 bodies after landslides devastated a road in western Cameroon with officials indicating there is little chance of finding survivors “There is no longer any possibility of finding survivors,” according to broadcaster CRTV The landslides struck the Dschang cliff road on Tuesday with a second landslide hitting as emergency teams used heavy equipment to clear debris Vehicles affected included three passenger coaches The last body was recovered on Saturday morning though the search continues for dozens still unaccounted for Cameroon’s roads are known to be treacherous, with an estimated 3,000 traffic-related deaths annually. The latest landslide disaster follows a tragic accident in early September when a tractor-trailer carrying passengers plunged into a ravine near Dschang, resulting in eight deaths and dozens of injuries A tragic road accident in western Cameroon has resulted in the deaths of eight people The accident occurred overnight on the outskirts of Dschang along a notorious stretch of road known as “the cliff,” which has a grim reputation for fatal crashes The large vehicle involved was traveling toward Douala when it reportedly malfunctioned and veered into a ravine Dschang regional hospital received the injured and confirmed the fatalities according to hospital director Michel Noubom though local public health official Daouda Chimoun stated that their conditions are no longer life-threatening This accident follows a pattern of deadly incidents on this road four people lost their lives in a similar accident at the Dschang “cliff,” and in January 2018 21 people were killed in another crash on the same stretch Cameroon’s roads are among the most dangerous in Africa, with over 6,000 people dying annually in traffic accidents according to the World Bank and the World Health Organization This translates to a rate of more than 30 deaths per 100,000 people placing Cameroon among the continent’s deadliest countries to drive in Cameroonian government figures are significantly lower with a transport ministry report indicating 963 road deaths in 2021 secretariat@globalinitiative.net Avenue de France 23 – Geneva, CH-1202 – Switzerland Nguiffeu Tajouo is a lecturer and researcher at the University of Dschang in Cameroon and a consultant to international and regional organizations such as UNODC He works on issues related to organized crime money laundering and the financing of terrorism After participating in expert workshops for the approval of the E4J University Modules he assisted the UNODC and ERSUMA (The Higher Regional School of Magistracy) in organizing the first summer university session on organized crime in French-speaking Africa in 2019 Nguiffeu Tajouo also wrote a report for the working group responsible for studying money laundering in the real estate sector in Central Africa with GABAC He has been working with SHERLOC/UNODC and CSU/UNODC for two years and is the author of several scientific publications National Research Institute of World Economy & International Relations (IMEMO) Programme Directior of Latin America and the Carribean secretariat@globalinitiative.net Avenue de France 23 – Geneva, CH-1202 – Switzerland Design e Creative Coding by Café An official website of the United States government A .gov website belongs to an official government organization in the United States ) or https:// means you’ve safely connected to the .gov website Share sensitive information only on official Emergency Information for American Citizens Keynote Speech by Ambassador Christopher J Members of the University Board of Directors in this beautiful amphitheater of the University of Dschang Thank you to Vice Chancellor Tsafack for the invitation It’s always so energizing for me to engage with students and faculty I’ve been fortunate to have visited the Universities of Buea and Maroua and the Cameroonian International Relations Institute (IRIC) but being here at the University of Dschang is particularly special given the close relationship between this institution and the U.S The University of Dschang has a long tradition of academic excellence and we are proud to have contributed to the creation of this remarkable institution through a decade-long partnership between USAID and the Government of the Republic of Cameroon USAID strove to more closely link university instruction with the real-world needs of Cameroonian farmers continue to play a critical role in today’s complex and rapidly changing environment and prepare members of the community to tackle local are the result of ingenious ideas and research born from these very classrooms and laboratories because we believe this is the best way to invest in the future where together our partnership is helping to build a deeper mutual understanding and saving lives I look forward to an interactive question-and-answer session afterwards The United States is a longtime friend and partner of Cameroon We continue to cooperate with Cameroon on a broad range of issues including health; trade and business growth; fighting piracy We help foster collaboration and strengthen linkages between U.S We provide access to free digital library resources to assist students and faculty to get the information they need We support projects to empower youth and develop their leadership skills across Cameroon every year close to 100 Cameroonian students and young professionals travel to the United States for educational and professional exchanges sponsored by the U.S who are giving back to their communities upon their return sharing what they learned in the United States and exemplifying the enduring partnership between our two countries We’re pleased that the University of Dschang has also taken advantage of these exchanges by welcoming American scholars and envoys in return Andrea Drager worked with Professor Marie Louise Avana and University of Dschang students on cutting-edge research in agroforestry American film director and producer Mark Warshaw visited the university’s Fine Arts Institute in Foumban and had a rewarding exchange with the students and faculty I’m particularly pleased to learn that the University of Dschang has partnerships with several U.S academic institutions including the University at Buffalo and the Kentucky Institute for International Studies It’s my hope that these partnerships continue to grow for the mutual benefit of Cameroonian and American students and scholars These exchanges and partnerships embody the U.S commitment and long-term work to invest in Africa’s tremendous human capital and in its institutions As President Biden said at the U.S.- Africa Leaders Summit last year: “African voices African innovation all are critical to addressing the most pressing global challenges.”  Important breakthroughs in medicine and climate change…  Amazing innovations in information technology and other 21st Century industries…  Tremendous entrepreneurship among women and young people…  These are already occurring across the continent and here in Cameroon and everyday citizens to facilitate this in countless ways Turning now to health…  Helping to improve health outcomes is a top priority for the United States and civil society spans all 10 regions of Cameroon this year we’re celebrating the 20th anniversary of PEPFAR—the United States President’s Emergency Plan for AIDS Relief the United States has invested more than 385 billion Francs CFA through PEPFAR making tremendous progress in the fight against HIV/AIDS and strengthening Cameroon’s health system This longstanding partnership also laid a critical foundation that helped Cameroon prepare and respond to other pandemics we support the Dschang Regional Hospital Annex to provide HIV testing the United States has invested more than 80 billion Francs CFA in Cameroon since 2017 helping to decrease child mortality by more than 35 percent a powerful example of the longstanding friendship and partnership between our two countries—Peace Corps Last September marked the 60th anniversary of Peace Corps Cameroon has seen more than 3,900 volunteers come here Many Cameroonians have told me about remarkable Peace Corps Volunteers who touched their lives and many remarkable Cameroonian communities have shaped the lives of Volunteers in return everything I’ve been talking about this morning is really about people Government might be — and has been — a positive actor and catalyst for that I would like to thank the university community for your commitment to excellence in your respective fields You can find more information here You can find more information 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