Metrics details
Ivermectin-based preventive chemotherapy (PC) is distributed annually to all at-risk populations eligible for ivermectin treatment to control and/or eliminate onchocerciasis
Information on the impact of mass ivermectin administration on onchocerciasis transmission is scanty
and it is tricky to appreciate the progress towards elimination and engage corrective measures
To fill that gap in the Centre Region in Cameroon
the current onchocerciasis endemicity level in the Ndikinimeki Health District after about two decades of mass treatments was assessed
A cluster-based cross-sectional survey was carried out in the Ndikinimeki Health District and all volunteers aged ≥ 5 years were (i) interviewed on their compliance to ivermectin over the past five years and (ii) underwent clinical (nodule palpation and visual search for onchocercal lesions) and parasitological examinations (skin snip) for onchocerciasis
The overall Onchocerca volvulus prevalence was 7.0% (95% CI: 5.2–9.3%)
The prevalence of the disease was significantly higher in the communities Kiboum 1 and Kiboum 2 compared to the other communities (highest prevalence in Makénéné Town Water: 8.5%; 95% CI: 2.3–20.4%) (χ2 = 51.314
The proportion of systematic non-compliers to ivermectin was 23.3% (95% CI: 19.9–27.1%) among individuals interviewed
In the sentinel sites (Kiboum communities)
onchocerciasis prevalence decreased from 95.2% (95% CI: 88.3–98.1%) to 23.7% (95% CI: 14.7–36.0%)
This study has revealed that the Ndikinimeki Health District is hypo-endemic for onchocerciasis after about two decades of preventive chemotherapy
with potential hotspots in the Kiboum 1 and Kiboum 2 communities
which are known as first-line communities (closest to the breeding sites of the vector)
Alternative or complementary strategies to annual ivermectin appear compulsory to accelerate the momentum towards onchocerciasis elimination
The pathogenesis of the diseases is essentially due to microfilariae
while adult worms usually induce no pathology or simply stimulate the development of characteristic subcutaneous nodules
In order to improve current control approaches and define a more pinpointed strategy
it is worth carrying out regular surveys to assess the impact of control approaches on the disease epidemiology
this study assessed onchocerciasis endemicity in the Ndikinimeki Health District after about two decades of CDTI
using a district-wide rather than sentinel site approach
A cross-sectional survey was carried out in the Ndikinimeki Health District following a cluster sampling approach
Clusters were selected using the probability proportionate to estimate size (PPES) strategy
In order to ensure that all clusters have the same probability of selection
communities (clusters) were organized into Health Areas prior to the PPES procedure to take into account the difference in the number of communities per Health Area and the difference in community size
A total of 12 clusters were selected by the PPES procedure
Considering the onchocerciasis prevalence (54.7%) in the Ndikinimeki Health District according to the most recent impact assessment survey (2011) by the National Onchocerciasis Control Programme (NOCP)
a minimal sample size of 381 was needed to estimate the true prevalence of onchocerciasis in 2019 in the Ndikinimeki Health District with 5% precision
Eligible participants were both males and females
and who had been living in the selected clusters for at least 5 years
and adherence to ivermectin treatments during the past 5 years preceding the survey
were collected from eligible participants using a structured questionnaire
individuals aged < 10 years were excluded from history and adherence to CDTI since they are less likely to provide accurate enough answers to such questions
All the individuals who accepted to participate underwent clinical and parasitological examinations
follow-up of the trend in onchocerciasis prevalence over three decades (or 25 years of annual mass ivermectin treatment) was carried out only in the Boutourou Health Area where a sentinel site (Kiboum communities) was identified during mapping exercise
based on the availability of baseline data
Both the baseline (1991) and the follow-up (2011) surveys were carried out by the NOCP
The number of microfilariae in the fluid was counted (when positive) and the individual microfilarial densities were computed as the arithmetic mean number of microfilariae in the two skin snips (mf/ss)
especially after multiple rounds of ivermectin treatments
Mann-Whitney and Kruskal–Wallis tests were used to compare onchocerciasis prevalence and mean microfilarial density between clusters
The geographical coordinates of each community visited were recorded using a high sensitivity global positioning system [GPS eTrex; Garmin (Europe) Ltd
A thematic analysis was performed using a geographical information system (GIS) software (ArcGIS
of onchocerciasis point prevalence in the Ndikinimeki Health District; three main layers (roads
the latest representing potential blackfly breeding sites) were used to create/illustrate the map
A total of 603 participants aged 5 to 88 (median: 32; interquartile range (IQR): 42) years were examined in 12 clusters (communities) of the six Health Areas of the Ndikinimeki Health District
The sex ratio (M/F) was 1.04 (proportion of males: 50.9%)
Onchocerca volvulus infection rate in the different communities surveyed in the Ndikinimeki Health District
The overall prevalence of palpable nodules was 0.3% (95% CI: 0.1–1.2%)
ranging between 0–0.6% but the difference was not significant between Health Areas (χ2 = 1.416
The overall prevalence of skin depigmentation and rashes was 3.0% and 0.3%
and no significant difference (P > 0.330) was found between Health Areas (χ2 = 5.762
The difference was also not significant between gender groups (χ2 = 0.154
the proportion of individuals affected was significantly higher in the Nyokon Health Area (26.9%; 95% CI: 16.8–40.3%) compared to the other Health Areas (χ2 = 21.925
the difference was also significant when considering clusters (χ2 = 92.731
P < 0.0001) and age groups (χ2 = 18.498
but the difference was not significant when considering gender (χ2 = 1.436
74.8% (95% CI: 71.2–78.1%) of participants reported that they have swallowed ivermectin at least once during the past 5 years
The proportion of individuals who reported that they have ingested ivermectin every year during the past 5 years was 34.5% (95% CI: 30.8–38.4%)
comparable between males (36.2%; 95% CI: 31.0–41.7%) and females (32.8%; 95% CI: 27.7–38.3%) (χ2 = 0.76
A significant increase in the trend of compliance with ivermectin treatment was observed between the age groups (χ2 = 64.08
P < 0.0001); 22.6% (95% CI: 16.3–30.4%) of participants aged 10–19 years (individuals < 10 years-old excluded)
20.4% (95% CI: 13.5–29.7%) of participants aged 20–34 years
44.0% (95% CI: 35.1–53.4%) of participants aged 35–49 years
and 60.3% (95% CI: 53.1–67.1%) of participants aged ≥ 50 years declared having taken ivermectin tablets during the past 5 years
The proportion of systematic non-compliers
that is those individuals who never ingested ivermectin tablets during the past 5 years
slightly higher in females (26.3%; 95% CI: 21.3–31.9%) than in males (20.4%; 95% CI: 15.9–25.7%) although statistically non-significant (χ2 = 2.5
a significantly higher proportion of non-compliance with ivermectin treatment was observed between the age groups (χ2 = 24.59
Participants aged < 34 years exhibited a higher proportion of non-compliance with ivermectin treatment compared to their older counterparts (χ2 = 64.08
31.6% (95% CI: 24.3–39.9%) of participants aged 10–19 years (participants < 10 years-old excluded)
36.6% (95% CI: 27.5–46.7%) of participants aged 20–34 years
15.6% (95% CI: 10.0–23.6%) of participants aged 35–49 years-old
and 15.2% (95% CI: 10.7–21.1%) of participants aged ≥ 50 years declared that they have never swallowed ivermectin tablets during the past 5 years
A significant decrease in the prevalence of onchocerciasis was observed, between the present findings (2019) and both baseline data (1991) (χ2 = 41.59, df = 1, P < 0.0001), and the first decade trend (2011) (χ2 = 15.25, df = 1, P < 0.0001) (Fig. 2).
Trends in Onchocerca volvulus infection rates between 1991 (baseline) and 2019 (follow-up) in the Ndikinimeki Health District
Both the baseline (1991) and the follow-up (2011) surveys were carried out by the National Onchocerciasis Control Programme (NOCP)
with the support of the African Programme for Onchocerciasis Control (APOC) (NOCP
onchocerciasis is known to be naturally cumulative
but treatments administered for 18–21 years might have disrupted this relationship
thus favoring the shift in prevalence peak among individuals highly exposed and poorly compliant with ivermectin mass treatments
In the current situation with the persistence of onchocerciasis in a restricted hotspot area
implementation of multiple annual rounds of CDTI complemented with localized vector control by using for example ground larviciding might help boost the momentum towards the elimination of onchocerciasis in the Ndikinimeki Health District
and serve as a proof of concept for strategy improvement for countrywide elimination of onchocerciasis
This study reveals that the Ndikinimeki Health District is hypo-endemic for onchocerciasis after about two decades of CDTI
Despite the low prevalence and intensity of the disease in the study area
hotspots for onchocerciasis transmission was found in the Boutourou Health Area (around Kiboum 1 and Kiboum 2 communities) where alternative treatment strategies might be useful to prompt the elimination of onchocerciasis
All data generated or analyzed during this study are included in this published article and its additional file
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The authors are thankful to the local authorities for their assistance during the implementation of this study
and to the population who willingly accept to participate in this study
This work was supported by authors’ personal funds; fees related for field work were covered by RAA
Centre for Research on Filariasis and other Tropical Diseases (CRFilMT)
RAA conceived the study and designed the experiments
analyzed the data and drafted the manuscript
LS conceived the study and designed the experiments
supervised and coordinated the study and helped to draft the manuscript
NHN conceived the study and designed the experiments and helped to draft the manuscript
JB collected field data and contributed to the preparation of the manuscript
RHB collected field data and contributed to the preparation of the manuscript
HCND conceived the study and designed the experiments
All authors read and approved the final manuscript
Ethical clearance was granted by the Centre Regional Ethics Committee for Human Health Research (N°667/CRERSH/2019) and Administrative authorization was granted by the Regional Delegation of Public Health and the Ndikinimeki Health District
After approval of the local administrative and traditional authorities
the objectives and schedules of the study were first explained to community leaders and to all eligible individuals
informed consents were obtained from participants or their parents or guardians (for minors) who agreed to participate
An individual barcode was attributed to each participant for anonymous data analysis
The authors declare that they have no competing interests
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations
Prevalence and intensity of onchocerciasis in the different clusters of the Ndikinimeki Health District
unless otherwise stated in a credit line to the data
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DOI: https://doi.org/10.1186/s13071-020-04387-6
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