Hercules the hawk is infatuated with Andi Billerbeck\u2019s hair during a program at the Amana library last week Home / Hometown Current as the Iowa Raptor Project visited the Amana Community Library Wednesday director of UI WILD (University of Iowa Wildlife Instruction and Leadership Development) takes his team to three or four sites a week during the summer They also give tours at Macbride Nature Recreation Area including the three they showed at the Amana library introduced her audience in Amana to North America’s smallest falcon Falcons are some of the fastest mammals in existence The colorful American kestrel can reach 40 miles an hour or better but the peregrine falcon can reach a speed of nearly 240 mph Martensen said she had trouble finding kestrels when she looked for them during the summer but in the winter she can spot them as they sit on electrical lines scanning empty farm fields for prey The colorful bars on the kestrels’ heads make them identifiable as falcons rather than hawks The kestrel has long narrow wings and tail to increase its speed though the hawk on her glove was silent during the program “and they’ll go divebombing to that prey,” said Martensen They lay their eggs in existing nests rather than making nests of their own Only 50-60% of falcons live through their first year The falcon perched on her hand during the Amana presentation was hit by a car and has wing damage The next bird the Iowa Raptors team introduced the children to a licensed falconer and volunteer with the Iowa Raptor Project Owls’ wings have serrated edges which allow them to fly silently so their prey doesn’t hear them coming The owl can’t move its eyes like humans can so the owl turns its head — up to 270 degrees — to look for prey Owls have more rods in the back of their eyes than have other animals allowing them to draw in more light and see well at night The flat face of an owl directs sounds to its ears One ear is higher on its head than the other The owl has the best hearing almost any animal It can find a mouse in a total absence of light Last on the schedule was Hercules the red-tailed hawk is darker than the Eastern red-tailed hawks seen in Iowa this time of year Named for the mascot of the University of Iowa Hercules lost an eye when her parents stepped on her in the nest She was the first hawk to fly over the University of Iowa’s Kinnick Stadium (The University of Iowa’s tiger hawk logo looks more like a falcon than a hawk She looked at the paper spheres hanging from the ceiling preened and grabbed at Billerbeck’s hair with her beak “She’s gotten much better at programs,” said Billerbeck “They’ll eat whatever they can catch.” The birds have exceptional eyesight and find their prey from high in the sky and they look a little bit like vultures in the sky,” said Billerbeck the call accompanying bald eagles is actually that of hawks Red-tailed hawks have become the voice actors for bald eagles The Raptor Center at Macbride has a bald eagle that is turning 40 Billerbeck had to find a sponsor to mentor her for two years a falconer can gather equipment and build a mews The house must be at least eight feet by eight feet and it must be inspected by the Department of Natural Resources Then the falconer can trap a bird and train it Falcons don’t love you like a dog will “My personal red-tailed hawk trained faster than my dog did,” said Billerbeck UI WILD conducts wildlife camps during the summer and School of the Wild during the school year though the wildlife camps are also held in four Iowa DNR State Parks Macbride Natural Recreation Area is owned by the Army Corps of Engineers and managed by the University of Iowa It’s named in honor of former University of Iowa president and renowned naturalist Thomas Huston Macbride a raptor rehabilitation and education program began Enclosures were constructed at Macbride Nature Recreation Area for raptors that could not be released into the wild The Iowa Raptor Project is open for self-guided tours from 6 a.m.-9 p.m It’s located at Macbride Nature Recreation Area The Iowa Raptor Project is an outdoor facility Many paths are gravel and there are no modern restroom facilities This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page 11.55/Teams link opens for online attendees Ascomycete yeasts have evolved a large set of small protein toxins to compete against fungi in the environment Previous research revealed that these toxins exhibit diverse modes of action indicating that the yeast toxicome might constitute a rich source of functionally diverse but yet-untapped antifungals In my talk I will exemplify my group’s research on the molecular functioning of these yeast-derived toxins their modularity and engineerability towards applications in food mycorrhizas have been unearthed as key players in terrestrial carbon sequestration a major ecosystem service increasingly at risk  We are currently filling gaps in our knowledge of the diversity abundance and influence of different mycorrhizal fungi across ecosystems belowground Northeast/North Central Nebraska’s most trusted Jane Frances de Chantal Catholic Church in Randolph Joseph’s Catholic Church in Pierce and the Rev Roger Schmit of Kansas City will officiate with Deacon Doug Tunink Johnson Funeral Home in Randolph is in charge of the arrangements at Trinity Lutheran Church in Madison with the Rev Burial will be at Crown Hill Cemetery in Madison Stonacek Funeral Chapel is in charge of the arrangements at the United Methodist Church in Meadow Grove of Norfolk are pending at Stonacek Funeral Chapel in Norfolk are pending at Stonacek Funeral Chapel in Norfolk Home for Funerals in Norfolk is in charge of arrangements of Howells are pending at Minnick Funeral Home in West Point Burial will follow at New Lutheran Cemetery in Norfolk NORFOLK — A celebration of life for Robert J at First Baptist Church in Norfolk with the Rev Inurnment will be at the Prospect Hill Cemetery in Norfolk GRAND ISLAND — A celebration of life for Elaine L at Peace Lutheran Church in the life center in Grand Island with the Rev WeatherThreat.com Closings Your browser is out of date and potentially vulnerable to security risks.We recommend switching to one of the following browsers: Account processing issue - the email address may already exist Invalid password or account does not exist Submitting this form below will send a message to your email with a link to change your password An email message containing instructions on how to reset your password has been sent to the email address listed on your account The legendary Billerbeck Bakery was not the only business in Freeport that bore the Billerbeck stamp There are people around who recall the downtown Freeport Hardware Co which carried two generations of Billerbecks to wealth and prestige Kathryn Cramer has fond memories of Jay and Myrtle Billerbeck and the hardware store Jay ran following his father Kathryn worked in the store as well as in Jay and Myrtle’s home at 1545 W Kathryn said the enormous brick house sits up higher than the street by the brick pillars which flank the entry of the driveway daughter of William and Edna (Wachlin) Borchardt said she and her mother worked together cleaning the Billerbeck home weekly for quite a few years She recalls there being a large cedar closet finished basement with a game room complete with flashing lights The couple owned 24 or 25 other properties “Everything in the house reeked like cigar and cigarette smoke Kathryn was very fond of both the Billerbecks Some people thought Jay was hard to get along with Kathryn remembers at the store wearing half-aprons with pockets holding a variety of tools and wire She alternated weeks between working at the store and at the home with her mother Kathryn’s mother would invite the elderly couple out to their home “for a day in the country “He would get up at 5:30 in the morning to be ready to go.” “He was especially nice to us,” Kathryn said She said his hands were crippled with arthritis which made it difficult for him to feed himself She remembered how very grateful to her he was when she gave him a cup with a handle in which his deformed fingers would fit In their day they put on lavish entertainment for the social elite of Freeport Myrtle became a recluse and would see no one Their friends fell away and they eventually “down-sized” their living quarters moving from their big house to an apartment above the building at the northwest corner of the intersection of Main Street and Galena Avenue That building had been the site of Billerbeck Bakery patronized by customers throughout northwestern Illinois When Kathryn Borchardt married Harry Cramer She was happy to have realized those dreams She said Jay and Myrtle Billerbeck were very supportive in her plans for the beautiful ceremony Myrtle bought her a pair of white net gloves and lent her the use of her silver service and white linen tablecloth Kathryn was very nervous about using the valuable items She still has the white gloves sentimentally stored away with other wedding memorabilia Kathryn made her own wedding gown which she said was of princess style made of white satin brocade with a rose design as well as the dress her mother wore for her and William’s 25th wedding anniversary Billerbeck also wished to contribute something to make her wedding special so he came up with a plan Kathryn’s mother wished to play her electric organ for the ceremony at Immanuel Lutheran Church in Lena but getting the organ there seemed to be impossible until Jay Billerbeck offered to transport it to the church and back in one of his trucks Kathryn’s mother was able to furnish the music for her daughter’s wedding These kindnesses from the Billerbecks remain etched in her memories to complement all those souvenirs she has tucked away The illustrious Billerbeck clan got its start in Freeport with the bakery Kathryn recalls her father remarking that no trip to Freeport as a child with his parents would have been complete without a stop at the Billerbeck Bakery for coffee and a roll    Freeport’s most famous bakery was begun about 1860 by John Billerbeck as a sideline to his small grocery store states the “History of Stephenson County 1970.” There was also a small restaurant associated with the bakery John Billerbeck had served an apprenticeship in Germany as a cracker baker and immigrated to Galena by way of New Orleans he came to Freeport and started a grocery business and small bakery continued to build up the business until they had one of the largest wholesale businesses in the region It is believed the Billerbeck Bakery instituted the production of pretzels which became the trademark for the city and the athletic teams of Freeport High School The bakery delivered its bread and other products throughout this northwest region Kathryn remembered the basement of the bakery building had long tunnels underground where the baking had taken place An excerpt from the 1970 history dates its beginning in 1872 by Arndt and Leemhuis Anton Billerbeck who had been a rival dealer joined the firm as manager about 1900 and soon bought out Dr Under his management and later that of his son the store expanded and prospered until 1968 when Jay Billerbeck retired due to ill health “liquidating the huge stock at the store drew customers from great distances,” the history states Writer’s Note: Today Kathryn keeps busy filling her many bird feeders daily year-round She loves to keep fancy chickens and has a dog Kathryn loves to watch her soaps and feels fortunate that her friends take her out to eat and to movies as well as to get groceries and pay bills She saw one film recently which she described as a “three tissue” experience She has her DVDs and her CDs and is nobody’s dummy Kathryn Cramer is sharp as a tack and a delight to talk with Her memories – like those about the Billerbecks – add fascinating color to the lives of our historic ancestry Metrics details An Author Correction to this article was published on 29 January 2019 This article has been updated Engineering multicellularity is one of the next breakthroughs for Synthetic Biology A key bottleneck to building multicellular systems is the lack of a scalable signaling language with a large number of interfaces that can be used simultaneously intercellular signaling language in yeast based on fungal mating peptide/G-protein-coupled receptor (GPCR) pairs harnessed from nature we assemble 32 functional peptide-GPCR signaling interfaces with a range of dose-response characteristics we demonstrate that these interfaces can be combined into two-cell communication links which serve as assembly units for higher-order communication topologies which we use to assemble three- to six-member communication topologies and a three-member interdependent community our peptide-GPCR language is scalable and tunable by genetic encoding and should be massively scalable by further application of our genome mining pipeline or directed evolution and inspired our effort to build an extensible communication language scaling the number of unique AHL ligand/receptor pairs by laboratory evolution requires the concerted engineering of AHL biosynthesis and receptor specificity SynNotch receptors are contact-dependent and therefore are only suitable for short-range communication which is conceptually different from long-range communication through diffusible signals we hypothesized that the peptide/GPCR-based mating language of fungi could be harnessed as an ideal source of modular parts for a scalable communication language the potential of leveraging the vast number of naturally evolved mating peptide-GPCR pairs as a scalable signaling language remains untapped c GPCRs are naturally orthogonal across non-cognate synthetic peptide ligands A 30 × 30 orthogonality matrix was generated by testing the response of 30 GPCRs across all 30 peptide ligands The test concentration was set at 10 µM of a given peptide ligand The fluorescence signal for maximum activation of each GPCR (not necessarily its cognate ligand) was set to 100% activation and the threshold for categorizing cross-activation was set to be ≥ 15% activation of a given GPCR by a non-cognate ligand GPCRs are organized according to a phylogenetic tree of the protein sequences d Orthogonality of peptide-GPCR pairs when peptides are secreted The 15 best performing pairs (marked in red in panels a–c) were chosen for secretion Experiments were performed by combinatorial co-culturing of strains constitutively secreting one of the indicated peptides and strains expressing one of the indicated GPCRs using GPCR-controlled fluorescent as read-out Experiments were performed in triplicate and results represent the mean While some GPCRs retained stable response parameters across a variety of peptide ligands most GPCRs’ response parameters could be modulated when exposed to these peptide variants these results imply the exciting opportunity to tune the response characteristics of a given GPCR by simply recoding the peptide ligand instead of engineering the receptor itself—a feature that can be exploited in future efforts The 15 peptides were chosen based on the favorable dose–response characteristics (low EC50 and high fold change) of the corresponding peptide-GPCR pairs c Overview of the implemented communication topologies Gray nodes: cells are able to process one input (expressing one GPCR) giving one output (secreting one peptide) Blue nodes: cells are able to process two inputs (OR gates expressing two GPCRs) giving one output (secreting one peptide) Orange nodes: cells constitutively secrete the peptide for the next clockwise neighbor and report on ring closure via a fluorescent read-out upon receiving a peptide signal from the counter-clockwise neighbor Red nodes: cells are able to receive a signal and respond via a fluorescent read-out d Ring topologies with an increasing number of members were established and error bars represent standard deviations The fold-change in fluorescence between the full-ring and the interrupted ring is indicated for each topology f A three-yeast bus topology (e) and a six-yeast branched tree-topology (f) were implemented (panel c) Fluorescence was measured after induction with all possible combinations of the three input peptides (zero The numbers above the bars indicate the fold-change in fluorescence over the no-peptide induction value Only the last yeast cell encoded a peptide-controlled fluorescent readout enabling measurement once information traveled successfully through the topology by comparing the fold change in fluorescence compared with not adding starting peptide which drastically limits the number of members that can be rapidly added to such a microbial community and suffer from a dependence on cross-feeding metabolically expensive molecules needed at substantial molar concentrations Our peptide–signal-based interdependence is conceptually different from cross-feeding metabolites as we use interfaces that are orthogonal to the cellular metabolism which allow scaling the number of community members by peptide–GPCR gene swapping and which are sensitive enough to function at low nanomolar signal concentrations The synthetic communication language enables construction of an interdependent microbial community a Illustration of the interdependent microbial communities mediated by the peptide-based synthetic communication language Peptide-signal interdependence was achieved by placing an essential gene (SEC4) under GPCR control and c3 secret the peptide needed for growth of the cx-1 member of the ring Peptides are secreted from the constitutive ADH1 promoter c Growth of the three-membered interdependent microbial community over > 7 days Communities with one essential member dropped out collapse after ~ 2 days (c) Three-membered communities were seeded in a 1:1:1 ratio controls were seeded using the same cell numbers for each member as for the three-membered community All experiments were run in triplicate and error bars represent the standard deviation d The composition of the culture was tracked over time by taking samples from one of the triplicates at the indicated time points plating the cells on media selective for each of the three component strains Inspired by the early impact of bacterial QS on our ability to engineer cell–cell communication and complex behavior we repurposed fungal mating peptide-GPCR pairs into a signaling language with a scalable number of orthogonal interfaces We demonstrate that the fungal pheromone response pathway naturally provides a large pool of unique signal and receiver interfaces that can be harnessed to build a modular these interfaces are readily accessible by genome mining as both the peptides and the GPCRs are genetically encoded and can be implemented by simple gene cloning and expression Genome mining alone yields a high number of off-the-shelf orthogonal interfaces whose component diversity can potentially be further scaled and tuned by directed evolution to exploit the full information density of the 9–13 amino acid peptide ligands (sequence space > 1014) the language can be tuned by ligand recoding as small changes in the sequence of a given peptide ligand alters the response behavior of a given GPCR changing the ligand sequence can be achieved by simple cloning and does not require receptor or metabolic engineering peptides are technically ideal as a signal Peptides are stable and rich in molecular information and virtually any short peptide sequence is readily available through commercial solid-phase synthesis allowing for the rapid characterization and evolution of new peptide-sensing mating GPCRs Escherichia coli was grown in Luria Broth (LB) media carbenicillin (Sigma-Aldrich) or kanamycin (Sigma-Aldrich) were used at final concentrations of 75–200 µg/ml and 50 µg/ml Agar was added to 2% for preparing solid yeast media clear-bottom 96-well microtiter plates were obtained from Corning (Corning Inc.) The custom code that was used for the programmatic retrieval of taxonomic information can be obtained from the authors upon request with no restrictions mating pheromone precursor genes have a relatively conserved architecture Genes encode for an N-terminal secretion signal (pre-sequence at the amino acid level) followed by repetitive sequences of the pro-peptide composed of non-homologous pro-sequences homologous sequences belonging to the presumptive signal peptide and protease processing sites the actual sequence of the secreted peptide ligand can be predicted from the precursor sequence Alignment with reported functional pheromone precursor sequences (from S We used the constitutive ADH1 promoter or the ligand-dependent FUS1 and FIG1 promoters to drive peptide expression For engineering yeast using the Cas9 system cells were first transformed with the Cas9 expressing plasmid followed by co-transformation of the gRNA carrying plasmid and a donor fragment Clones were then verified using colony PCR with appropriate primers strains were cured from the gRNA vector and directly used for deleting the next gene GPCR activity and response to increasing the dosage of synthetic peptide ligand was measured in strain JTy014 using the genomically integrated FUS1-promoter-controlled coRFP as a fluorescent reporter JTy014 strains carrying the appropriate GPCR expression plasmid were assayed in 96-well microtiter plates using 200 μl total volume Cells were seeded at an A600 of 0.3 (note: all herein reported cell density values are based on A600 measurements in 96-well plates of a 200 μl volume of cultures with a path length of ~0.3 cm performed in an Infinite M200 plate reader from Tecan) in SC media lacking uracil (selective component) All measurements were performed in triplicates emission: 620 nm) and culture turbidity (A600) were measured after 8 h using an Infinite M200 plate reader (Tecan) Since the optical density values were outside the linear range of the photodetector all optical density values were first corrected using the following formula to give true optical density values: Dose–response was measured at different concentrations (11 fivefold dilutions in H2O starting at 40 μM peptide H2O was used as no peptide control) of the appropriate synthetic peptide ligand All fluorescence values were normalized by the A600 and plotted against the log(10)-converted peptide concentrations Data were fit to a four-parameter non-linear regression model using Prism (GraphPad) in order to extract GPCR-specific values for basal activation Fold-activation was calculated for each GPCR as the maximum A600-normalized fluorescence of peptide-treated cells divided by the A600 normalized fluorescence value of water-treated cells GPCR activation was individually measured in 96-well microtiter plates in triplicate using each of the synthetic peptides (10 μM) Cells were seeded at an A600 of 0.3 in 200 μl total volume in 96-well microtiter plates Endpoint measurements were taken after 12 h Percent receptor activation was calculated by setting the A600-normalized fluorescence value of the maximum activation of each GPCR (not necessarily its cognate ligand) to 100% and the value of water-treated cells to 0% JTy014 was transformed with the appropriate GPCR expression plasmid and resulting strains were used as sensing strains yNA899 was transformed with the appropriate peptide secretion plasmids and used as secreting strains Sensing strains for all 16 peptides were individually spread on SC plates 0.5% agar was melted and cooled down to 48 °C cells are added to an aliquot of agar in a 1:40 ratio (100 μL of cells into 4 mL of agar for a 100 mm petri dish and 200 μL of cells into 8 mL of agar for a Nunc Omnitray) and poured on top of a plate containing solidified medium A 10 μL dot of each of the secreting strains was spotted on each of the sensing strain plates Plates were incubated at 30 °C for 24–48 h and imaged using a BioRad Chemidoc instrument and proper setting to visualized RFP signal (light source: Green Epi illumination and 695/55 filter) We examined peptide secretion in liquid culture by co-culturing a secreting and a sensing strain (expressing the cognate GPCR) and measuring fluorescence of the induced sensing strain Peptide secretion was under control of the constitutive ADH1 promoter Secretion strains for each peptide were constructed by transforming yNA899 with the appropriate peptide expression construct (pRS423-ADH1p-xy.Peptide) along with an empty pRS416 plasmid Sensor strains were constructed by transforming JTy014 with the appropriate GPCR expression construct (pRS416-TDH3p-xy.Ste2) along with an empty pRS423 plasmid Matching the auxotrophic markers of the secretion and sensor strains allowed for robust co-culturing Secreting and sensing strains were seeded in a 1:1 ratio each at an A600 of 0.15 and A600 and red fluorescence were measured after 12 h An unpaired t test was performed for each peptide with an alpha value=0.05 to determine if differences in secretion between constructs containing or not containing the Ste13 processing site were significant A single asterisk indicates a P-value < 0.05; a double asterisk indicates a P-value < 0.01 The same sensing and secreting strains as described for the “Peptide secretion liquid culture assay” (above) were used to confirm orthogonality of secreted peptide in co-culture Only the constructs that retained the Ste13 processing site were used each of the 16 constructed secretion strains were co-cultured 1:1 each at an A600 of 0.15 with the corresponding sensor strains to test for GPCR activation by non-cognate peptide and A600 and red fluorescence were measured after 14 h Percent activation of the sensor strain was normalized by setting the maximum observed activation of the sensor strain (not necessarily by the cognate ligand) to 100% and setting the basal fluorescence from co-culturing each sensor strain with a non-secreting strain to 0% activation yNA899 with the appropriate GPCR integrated into the Ste2 locus using the CRISPR system (described above) was transformed with the appropriate peptide secretion plasmid (pRS423-FIG1p-xy peptide retaining the Ste13 processing site) and the resulting strains were used as cell 1 (c1 JTy014 was transformed with the appropriate GPCR expression plasmid (pRS416-TDH3p-xy.Ste2) and used as cell 2 (c2 As c1 and c2 didn’t have the same auxotrophic markers validated strains were grown overnight in selective media and then seeded at a 1:1 ratio each at an A600 of 0.15 in SC media Cells were cultured in a total volume of 200 μl in 96-well microtiter plates and c1 was induced with the appropriate synthetic peptide at 2.5 nM Red fluorescence and A600 were measured after 12 h c2 was co-cultured with a non-secreting strain carrying an empty pRS423 plasmid and induced with the appropriate synthetic peptide at the concentrations listed above Communication loops were designed so that a single fluorescent measurement would indicate signal propagation through the full ring topology An initiator strain was constructed by integrating the Ca.Ste2 into JTy014 and transforming it with a constitutive Kp peptide secretion plasmid (pRS423-ADH1p-Kp.Peptide) Linker strains from the transfer functions experiment (without a fluorescent readout) were used to complete each communication ring Communication rings were seeded in triplicate at equal ratios (A600 = 0.02 each) in 10 mL selective 2x SC–His medium and incubated at 30 °C with 250RPM shaking for 36 h 200 μL samples were taken for a fluorescent measurement of red fluorescence (588 nm/620 nm excitation/emission) in technical triplicate in a 96-well black clear-bottom plate and normalized by A600 To demonstrate that communication is contingent on a complete ring topology a control with the first linker yeast strain in each ring dropped out was performed in parallel The panels compare the normalized red fluorescent signal for each ring to the dropout control with the fold-change induction of the completed ring indicated Fold change compared with no added peptide is indicated Cells were exposed to the indicated peptide concentrations and cultured for 12 h in 96-well microtiter plates in a total volume of 200 μl at 30 °C and 800 rpm shaking 50,000 cells were analyzed using a BD LSRII flow cytometer (excitation: 594 nm The fluorescence values were normalized by the forward scatter of each event to account for different cell size using FlowJo Software and ySB188 were maintained on SD agar plates supplemented with 1 µM of Ca For assaying their peptide-dependent growth response strains were cultured overnight in the presence of 100 nM peptide in SC–His Cells were washed five times with one volume of water Cells were then seeded in 200 μl SC (no selection) at an A600 of 0.06 and cultured at 30 °C and 800 rpm shaking Cells were exposed to different concentrations of peptide (seven 10-fold dilutions starting from 1 μM water was used for the “no-peptide” control) A600 was determined at various time points over the course of 24 h The 24 h-data points were plotted against the log10 of the peptide concentrations Data were fit to a four-parameter non-linear regression model using Prism (GraphPad) to extract values for peptide/growth EC50 serial 10-fold dilutions of overnight cultures of ySB270 and ySB265 were spotted on SD agar plates supplemented with or without 1 μM peptide and incubated at 30 °C for 48 h and ySB188 were transformed with the appropriate peptide secretion vectors (Bc or Vp1) featuring peptide expression under the constitutive ADH1 promoter the resulting peptide-secreting strains (treated with peptide and washed as described above) were seeded in the appropriate combination in a 1:1 ratio in 200 μl SC–His at an A600 of 0.06 (0.03 each) and cultured at 30 °C and 800 rpm shaking The same cell number of single strains was seeded alone and cultured in parallel as control A600 measurements were taken at the indicated time points and cultures were diluted into fresh media when the culture reached an A600 of 0.8 -1 the appropriate peptide secreting strains (c1 and c3) were inoculated in a ratio of 1:1:1 in 200 μl SC–His media at an A600 of 0.06 (0.02 each) in a 96-well plate cultured at 30 °C and 800 rpm shaking All three combinations of controls lacking one essential member (c1 omitted A600 measurements were taken at the indicated time points and cultures were diluted 1:20 into fresh media approximately every 12 h the dilution rate was reduced to 1:20 every 24 h Samples were used to determine the co-culture composition and the peptide concentration Deconvolution of strain identity: aliquots of the culture were plated on three different plate types Each strain can only grow on plates containing its cognate peptide ligand The co-culture composition was than determined by colony counting Peptide concentration: We used JTy014 transformed with the appropriate GPCR as peptide sensor The linear range of the GPCR dose response was used for peptide quantification The authors declare that all the data supporting the findings of this study are available within the paper and its supplementary information files or from the authors upon reasonable request The original version of this Article omitted a declaration from the Competing Interests statement which should have included the following: ‘J.D.B is a founder and Director of the following: Neochromosome the Center of Excellence for Engineering Biology and serves on the Scientific Advisory Board of the following: Modern Meadow This has now been corrected in both the PDF and HTML versions of the Article The origins of multicellularity and the early history of the genetic toolkit for animal development The involvement of cell-to-cell signals in the development of a bacterial biofilm Quorum sensing controls biofilm formation in Vibrio cholerae Bacteria-host communication: The language of hormones Multi-species biofilms: living with friendly neighbors An integral program for tissue renewal and regeneration: Wnt signaling and stem cell control Quorum sensing: cell-to-cell communication in bacteria Cellular control of the synthesis and activity of the bacterial luminescent system A synthetic multicellular system for programmed pattern formation Programmable cells: interfacing natural and engineered gene networks Robust multicellular computing using genetically encoded NOR gates and chemical ‘wires’ Programmed population control by cell-cell communication and regulated killing Synchronized cycles of bacterial lysis for in vivo delivery Emergent genetic oscillations in a synthetic microbial consortium Use of bacterial quorum-sensing components to regulate gene expression in plants eukaryotic gene expression system based on the quorum-sensing transcription factor TraR (vol 4 Quorum sensing communication modules for microbial consortia Refinement and standardization of synthetic biological parts and devices Can the natural diversity of quorum-sensing advance synthetic biology Dual selection enhances the signaling specificity of a variant of the quorum-sensing transcriptional activator LuxR (vol 24 Synthetic quorum sensing and cell-cell communication in Gram-positive Bacillus megaterium Altering the communication networks of multispecies microbial systems using a diverse toolbox of AI-2 Analogues Bacterial interference caused by autoinducing peptide variants The LuxS family of bacterial autoinducers: biosynthesis of a novel quorum-sensing signal molecule Developing next generation antimicrobials by intercepting AI-2 mediated quorum sensing Interference with AI-2-mediated bacterial cell-cell communication Insightful directed evolution of Escherichia coli quorum sensing promoter region of the lsrACDBFG operon: a tool for synthetic biology systems and protein expression Engineering customized cell sensing and response behaviors using synthetic Notch receptors A modular yeast biosensor for low-cost point-of-care pathogen detection Fungal mating pheromones: choreographing the dating game Magnificent seven: roles of G protein-coupled receptors in extracellular sensing in fungi A yeast pheromone-based inter-species communication system Secreting and sensing the same molecule allows cells to achieve versatile social behaviors Distributed biological computation with multicellular engineered networks Mutagenic mapping of helical structures in the transmembrane segments of the yeast alpha-factor receptor Sequences in the intracellular loops of the yeast pheromone receptor Ste2p required for G protein activation A new purple fluorescent color marker for genetic studies in Saccharomyces cerevisiae and Candida albicans Engineering cell sensing and responses using a GPCR-coupled CRISPR-Cas system chemical sensors for medium-chain fatty acids Yeast alpha mating factor structure-activity relationship derived from genetically selected peptide agonists and antagonists of Ste2p Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker’s yeast Synthetic microbial consortia enable rapid assembly of pure translation machinery Principles for designing synthetic microbial communities Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production Austin, H. P. et al. Characterization and engineering of a plastic-degrading aromatic polyesterase. Proc Natl Acad Sci USA (2018), https://doi.org/10.1073/pnas.1718804115 Low escape-rate genome safeguards with minimal molecular perturbation of Saccharomyces cerevisiae Shaw, W. M. et al. Engineering a model cell for rational tuning of GPCR signaling. Preprint at https://www.biorxiv.org/content/early/2018/08/13/390559 Production of the antimalarial drug precursor artemisinic acid in engineered yeast Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems Methods in yeast genetics: a Cold Spring Harbor Laboratory course manual The InterPro protein families database: the classification resource after 15 years scalable generation of high-quality protein multiple sequence alignments using Clustal Omega Causes and consequences of variability in peptide mating pheromones of Ascomycete Fungi Enzymatic assembly of DNA molecules up to several hundred kilobases Yeast Golden Gate (yGG) for efficient assembly of S Tunable and multifunctional eukaryotic transcription factors based on CRISPR/Cas and red fluorescent protein tagging vectors for S A synthetic biology framework for programming eukaryotic transcription functions Download references This research was partly funded by DARPA award HR0011-15-2-0032 and NIH award 5R01AI110794 was supported by a Simons Junior fellow award from the Simons Foundation were supported by NSF Graduate Research Fellowships (DGE 16–44869) Part of the research reported in this publication was performed in the CCTI Flow Cytometry Core at Columbia Medical Campus supported in part by the NIH Office of the Director The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH Present address: The Koch Institute for Integrative Cancer Research These authors contributed equally: Sonja Billerbeck Institute for Systems Genetics and Department of Biochemistry and Molecular Pharmacology conceived and conceptualized the idea that the fungal peptide-GPCR pairs could be a scalable language and provided preliminary data for the scalable language performed programmatic retrieval of GPCR and peptide genes and extracted candidate signal peptide sequences performed the multi-yeast communication experiments performed peptide-signal dependent co-culture experiments designed the experiments and analyzed the data wrote the paper with the help of all authors A provisional patent application (62/516,383) was filed June 7 as inventor and assigned to The Trustees of Columbia University in the City of New York The remaining authors declare no competing interests Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41467-018-07610-2 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology The Portland chip company has largely missed out on the chip industry's robust growth since the Great Recession .st1{fill-rule:evenodd;clip-rule:evenodd;fill:#2a2a2a}By Mike Rogoway | The Oregonian/OregonLiveDarin Billerbeck Less than a week after activist investors placed new directors on Lattice Semiconductor's board the Portland company said longtime chief executive Darin Billerbeck will retire moving Lattice from its roots in Hillsboro to new headquarters in downtown Portland The company makes programmable computer chips used in everything from smartphones and digital cameras to communications equipment Lattice is Portland's biggest tech company but is relatively small by chip industry standards It reported $386 million in revenue last year The company has lost money in each of the past three years Lattice's fortunes improved modestly under Billerbeck but the company largely missed out on the broad run-up in tech stocks since the Great Recession Lattice's share price rose 32 percent during Billerbeck's tenure; during the same period an index of 30 other chip stocks (not including Lattice) rose nearly 240 percent Lattice's shares fell 8 cents in early trading Tuesday to $6.40 Global attention focused on Lattice in 2016 and 2017 as Chinese-backed investors bid $1.3 billion for the company The deal became a test of the U.S government's appetite for Chinese investment in the tech sector and President Donald Trump ultimately blocked the transaction activist investors with Lion Point Capital acquired a 7 percent stake in the company and began agitating for a board shakeup Lattice agreed to add three directors from Lion Point to its eight-member board Lattice vice president Doug Hunter said Billerbeck's exit is unrelated to Lion Point's involvement While Billerbeck had originally been planning to step down late next year Hunter said that thinking changed recently as Lattice began to push into new networking technologies and embedded devices known as the Internet of Things Hunter said Billerbeck and his family decided this was a natural time for a transition "Darin's been working hard and traveling hard for the past 30 years," Hunter said "We're just at a place where I think he said Susquehanna International Group analyst Christopher Rolland wrote that he suspects there's more to the story "We can imagine that 'rebooting' Lattice while simultaneously managing an activist investor could be a challenging process and one in which Mr Billerbeck was unwilling to accept," Rolland wrote "This moment was as good as any to negotiate an exit and announce retirement." Despite the failed Chinese bid for Lattice Rolland wrote that Lattice remains a good candidate for a buyout Folding Lattice into a larger company could reduce expenses associated with being a small publicly traded chip company and double its earnings power Rolland said Lattice is unlikely to attract a bid close to the $8.30 per share that Chinese investors had offered Billerbeck will formally retire as CEO and member of the board on Friday though he will remain an executive until the end of May Lattice said it will pay him $1 million in severance immediately vest 35,000 units of restricted stock and pay for up to a year of health care Lattice named Chief Operating Officer Glen Hawk its interim CEO and said it will pay him an additional $15,000 a month while he remains in that role Hawk lives in California and works at a Lattice office there and Hunter said he will not move to Portland - but will likely be spending more time here The Portland company said it hired a search firm Hunter said that Lattice had recruited Hawk as a potential successor to Billerbeck and that Hawk will be a candidate to take over the job in the long term "The board said that they're committed to finding the best candidate they can," Hunter said "and that would include internal and external candidates." Correction: This article has been corrected to indicate that Glen Hawk is Lattice's chief operating officer This article has been updated with additional comment -- Mike Rogoway; twitter: @rogoway; 503-294-7699 Use of and/or registration on any portion of this site constitutes acceptance of our User Agreement, (updated 8/1/2024) and acknowledgement of our Privacy Policy, and Your Privacy Choices and Rights (updated 1/1/2025) © 2025 Advance Local Media LLC. All rights reserved (About Us) The material on this site may not be reproduced except with the prior written permission of Advance Local Community Rules apply to all content you upload or otherwise submit to this site YouTube's privacy policy is available here and YouTube's terms of service is available here Ad Choices NEW BERLIN — New Berlin High School will host Pawnee in a non-conference baseball game today Pawnee is among 24 Illinois high schools that carry the “Indians” nickname two schools sharing one of the nation's most distinctive nicknames were on the baseball field at the same time “There’s a lot of pride in that name,” said Don Parker pitch for Freeport in a 10-4 win over New Berlin I didn’t know there was another one until they set up this game.” The communities are more than 200 miles apart is in Stephenson County in northwestern Illinois According to “Why Mascots Have Tales,” a book written by Fred Willman and published in 2005 New Berlin and Freeport are the only schools in the United States whose nickname can be crunchy or soft Freeport first baseman Travis Buckwalter said he'd never trade his identity as a Pretzel — or “Pretz” as Freeport’s teams are called up north “I’ve talked to a lot of people who’ve graduated (from FHS) and they say they’ll always be Pretzels,” said Buckwalter Freeport’s Pretzel story is more definitive than New Berlin’s although the latter town’s name alone reveals its German heritage A local newspaper dubbed Freeport “The Pretzel City” in the 1890s due in part to the city’s German population and the presence of Billerbeck Bakery which was the city’s biggest producer of pretzels “There were breweries and a pretzel factory; it was a German town,” Parker said “I guess they chose Pretzels instead of Brewers.” The most accepted story on the birth of the New Berlin Pretzels centers around a New Berlin basketball game in the 1920s or '30s and they were tossed to the players on the bench A sports writer or game announcer called them “pretzel tossers,” and the rest is salty history “The nickname’s definitely different,” said New Berlin coach Joe Kindred Kindred said Fegan contacted New Berlin about the possibility of a game this spring and Freeport already was planning a trip “down south.” Freeport played on Monday at Lenz Field in Jacksonville “We’ve been bumping up our schedule with bigger schools,” Kindred said “Add in the nuance of them and us both being Pretzels I jokingly called it the Pretzel National Championship.” New Berlin gave it the big-game treatment Tuesday and all of the players were introduced before the game brought a trophy — which it displayed when both teams gathered for a group picture in center field after the game Fegan referred to it as a “traveling trophy,” a hint that the schools could meet on an annual basis although he said logistics and weather could pose challenges  New Berlin senior outfielder Jake Hunt was grateful to face off against the northern Illinois half of the Pretzel brotherhood in his final season you think you’re the only ones in the world because it’s so unique,” Hunt said “Knowing there’s only one other Pretzel team besides us and being able to wear the Pretzel uniform for four years — A man died in a motorcycle crash Wednesday when the driver had a medical emergency according to the Allegan County Sheriff's Office It happened at 58th Street near 130th Avenue north of Fennville around noon was driving north on 58th Street and rounding a curve when a witness said he ran off the road The preliminary investigation indicates Billerbeck may have suffered a medical event before the crash The Allegan County Sheriff’s office reconstruction team is currently investigating the crash The Sheriff’s office was assisted on scene by Life EMS Michigan State Police and Fennville Police Grandparents are Camron and the late Lori Means of Norfolk and Michael and Becky Lechner of Norfolk Great-grandparents are Dick and Judy Danielson of … The family of Marilyn Glaser would like to honor her with a card shower for her 85th birthday on Tuesday her husband of 65 years; son Brad Glaser (Gina) of Norfolk; daughter Diana (Bryce) Williams of Eddyville; son Monte Glaser (the late Phyllis G… We accept many different kinds of announcements Just click on the button below and submit a form Please select what you would like included for printing: Copy the text below and then paste that into your favorite email application NE.  She attended Walthill Public School then went to East High School in Sioux City for her junior year.  Donna returned to Walthill High School her senior year and graduated with the Class of 1951.  In October of 1951 she started working as a switchboard operator/supervisor at Northwestern Bell.  In 1953 Donna survived the Floyd River Flood in Sioux City Donna met Wilbur Eugene Lieber at the Tomba Ballroom in Sioux City.  They were united in marriage on February 19 1955 at Redeemer Lutheran Church and this union was blessed with five children.  In 1962 Donna left her job with the telephone company to raise her family on the family farm near Sioux City.  From 1990 to 2000 she worked at JCPenney in the catalog and credit department    Donna’s memberships included Calvary Lutheran Church and Willing Workers Neighborhood Club.  She enjoyed sewing and canning.  Donna was an excellent cook and enjoyed making brownies and apple pies for her grandchildren.  In addition she baked and decorated many cakes for different celebrations.  She also enjoyed going on several trips with Wilbur and going every Friday to get her hair done.  Above all she cherished her family.  She had a great memory and would acknowledge all of her family with birthday and holiday cards    Donna is survived by five children: Richard (Debbie) Lieber of Lawton Roger (Holly) Lieber of Lawton and Renee (Kory) Eyres of Lawton; 12 grandchildren: Dustin (Melissa) Lieber Allie Lieber and Rylee Eyres; six step grandchildren: Iris Margellos Sam Billerbeck and Zach Rysavy; 16 great grandchildren: Adrian and Jadynn Lieber and Liam Speulda; and many other relatives and friends    She was preceded in death by her parents; husband of 65 years This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply Service map data © OpenStreetMap contributors By 2024-04-22T11:50:00+01:00 Source: Manfred Werner (licensed under CC BY-SA 3.0) / Harald Krichel (CC BY-SA 3.0) April 22) in Hamburg on Fatih Akin’s Second World War drama Amrum Laura Tonke and Diane Kruger leading the cast Beta Cinema has boarded the film and will launch international sales in Cannes next month The film is produced by Akin’s own company bombero international with Warner Bros Film Productions Germany Warner Bros Pictures will release the film in Germany in September 2025 Written by Akin and his In The Fade co-writer Hark Bohm Amrum is set on the eponymous German island in spring 1945 as a 12-year-old boy helps his mother feed their family in the final days of the Second World War until the arrival of peace brings new conflicts The story is based on German filmmaker Bohm’s own memories Billerbeck plays the young boy with Tonke as his mother and Kruger as the wife of a local farmer Screen was first to report on the project in 2022; it is the first title under Akin’s multi-year first-look deal with Warner Bros Backers include the German Federal Film Board Moin Filmforderung Hamburg Schleswig-Holstein the Federal Government Commissioner for Culture and Media MFG Baden-Wurttemberg and the Bavarian Film Prize Akin’s previous productions through bomber international include Venice 2014 title In The Cut and Cannes 2016 entry In The Fade for which Kruger won best actress on the Croisette Akin said Amrum “began as a Hark Bohm film [and] now becomes my 12th feature film and an extraordinary mission” EXCLUSIVE: Film will release in cinemas this summer Screen reveals a snapshot of the latest high-end TV and film productions shooting in the UK for the big studios and streamers EXCLUSIVE: Film shoots in Dublin and Dundalk this summer Bookmark this page and keep track of the latest film release dates in the UK & Ireland Bookmark this page to keep track of all the latest festival dates EXCLUSIVE: Eva Victor’s comedy drama world premiered at Sundance and will play at Cannes in Directors’ Fortnight Screen International is the essential resource for the international film industry access to the Screen International archive and supplements including Stars of Tomorrow and World of Locations Site powered by Webvision Cloud The only instruction aid most golfers would ever need is three pieces of hard-stock paper bound together by one fastener that has tips from one of the greatest players of all-time It was released in 1966 under the name “Team up with Arnold Palmer.” whose stint as the physical education teacher at Gibraltar High School preceded that of my father Billerbeck was a local baseball legend who also loved golf working in a pro shop and grinding on his game during the summer When my dad moved into Billerbeck’s old office he found a small box of golf goods that were left over tees — the usual stuff — and one more thing: an instruction slide wheel from Arnold Palmer Enterprises It offers 360 degrees of tips on 36 different shots (plugged lie as well as causes and corrections for 10 of your most typical screw-ups (slice For the golfing individual who doesn’t want lessons doesn’t think they need lessons or cannot afford lessons it was AND IS a legitimately useful instrument to bring to the range but be sure to contact ball before hitting the bottom That’s just three of the clues tucked within Flip the diagram around and you’ve got fixes for potentially bigger issues in your game As someone who recently hit a perfect draw one day after receiving a lesson on how to do so I have rarely felt more powerful on the course I proceeded to ignore the game for two months (winter problems) and forgot most of the aspects that helped me hit the draw To hit a low shot: “Play ball farther back To hit a high shot: “Play ball forward My result: A 7-iron that soared straight and dropped down at 154 yards Followed by How to Hook and the other once- or twice-a-round miracle shots you’d love to pull off with more regularity the issue isn’t as much the action involved with shaping shots or fixing my swing on the fly It’s more about remembering exactly what is important Arnie’s diagram is simply an adult version of the mental math flashcards we did in elementary school At its core, the dial is a memory device. Mastery of the shots is entirely optional and entirely up to you. If you’re quick enough, there are a couple available on eBay To receive GOLF’s newsletters, subscribe for free here All of our market picks are independently selected and curated by the editorial team You are using an outdated browser. Please upgrade your browser to improve your experience The Athens County Historical Society and Museum will be a winter wonderland throughout December she saw a documentary for a gingerbread competition and noticed how people’s faces lit up when looking at the entries “How would that idea work in Athens?” she wondered Hodson then teamed up with her childhood friend Joyce Mullins to kick off the Uptown for the Holidays Gingerbread House Display and Competition Hodson and Mullins have added categories to accommodate participants and their ideas they separated a professional category from a non-professional category so that local chefs and bakers would not have any unfair advantages they added categories so that those who do not wish to submit an Athens building replica — as winners were required to do — can still participate Hodson has seen replicas of buildings such as Cutler Hall and fictional structures such as Hagrid’s Hut from Harry Potter and a pirate ship the gingerbread houses are being displayed in one place rather than various uptown shops and businesses The Athens County Historical Society and Museum is featuring all of the houses through Dec Seventeen contestants dropped off their gingerbread houses on Tuesday and a panel of judges selected the winners: • Winner of the $500 Grand Prize Professional Category: Nancy Mingus • Winner of the $500 Grand Prize Non-Professional Category: Sarah Boumphrey • Child First Place: Sam Billerbeck Popoae The Athens County Convention and Visitors Bureau donated one of the grand prizes of $500 and gave Athens gift baskets to all second place winners gingerbread houses are associated with Christmas but Hodson said that doesn’t have to be the case which is why Kristin Miller created Chateau Shalom the Group First Place winner with her three-year-old son For Jack’s first gingerbread house experience Miller said she wanted him to learn more about Hanukkah because Jack’s father’s side of the family is Jewish “We just thought we would take it in a different direction,” Miller said “We already had our Christmas tree up…(Chateau Shalom) helps to celebrate Hanukah.” since their Jewish side of the family is in Phoenix and they wouldn’t be visiting this year they found a way to celebrate together at home some contestants pick up their creations to take home while others have them thrown out she and Jack would love to bring Chateau Shalom home Another house that will come home is a Wizard of Oz-themed gingerbread display a teacher at the Ohio University Child Development Center entered individually with a replica of the Ohio University President’s Residence but also entered her preschool students because they all had a knack for baking and have shown a great deal of enthusiasm for Gingerbread Man stories split up into groups to make different parts of Oz Mazzeo Barron said the children were “almost unanimous” on the Oz replica after the class had read the book and listened to the music together roughly 18 inches by 18 inches — and every inch is covered — includes the Emerald City which has fallen on the Wicked Witch of the East “We’re definitely going to bring it back and have it in our school along with some photos of every step of the process,” said Mazzeo Barron “Every child was really involved in multiple steps of the process.” Hodson is continually impressed with the community contributions to the event “These entries are just amazing every year and that’s the fun of it…the creativity involved and the hard work,” Hodson said “It’s really fulfilling for me to see that.” With Memorial Day weekend comes an influx of campers boaters and visitors to Johnson County’s Lake Macbride and Coralville Lake Home / News boaters and visitors to Johnson County's Lake Macbride and Coralville Lake But if you're heading out and plan to imbibe you might want to check the alcohol rules before cracking open a cold one Because a number of different agencies — including county state and federal — have jurisdiction over different areas of these popular hang-outs the rules pertaining to alcohol can literally change by walking a few feet from one place to another it's OK to drink a beer in the day-use area of Sandy Beach at Coralville Lake You can drink wine with less than a 17-percent alcohol content at the Lake Macbride campgrounds And if you're recreating at West Overlook at Coralville Lake you are not allowed to drink on land no matter where you are Lake Macbride park ranger with the Iowa Department of Natural Resources says officers plan to be on the lookout for offenders 'We're going to be out in full force this weekend,' said Rocca 'It just helps things move smoothly and helps make sure everybody is safe and also following all the rules of the park.' The Army Corps of Engineers owns the nearly 25,000 acres of land and water that makes up Coralville Lake and its surrounding public use areas said the Corps is not an enforcement agency but officials can ask unruly guests to pour out their alcohol or leave and issue trespassing citations if necessary the Corps defers to the Johnson County Sheriff's Office to enforce laws The federal entity contracts with the county office for a regular presence in the area 'If the Corps of Engineers gets a complaint of alcohol on the beach we would assist them and explain to the people that they need to remove the alcohol or leave,' Johnson County Sheriff Lonny Pulkrabek said The sheriff's office also deploys a patrol boat for added coverage on the water during busy weekends Alcohol has been banned on the lake's three main beaches — West Overlook Sandy Beach and Sugar Bottom — since 2004 drunken parties at West Overlook's day-use area and banned alcohol beyond the beaches to include the entire West Overlook area 'It was truly a line in the sand,' Goldman said of the alcohol-free zone 'We started to see a lot more problems growing with larger groups of people that would be off the sand Alcohol still is allowed off the sand — in the nearby campgrounds lodges and parks — at Sugar Bottom and Sandy Beach Just to the east of Coralville Lake is Lake Macbride Rules at the surrounding state park are enforced by park rangers and conservation officers with the Iowa Department of Natural Resources Hard alcohol is prohibited in all Iowa state parks and only beer is allowed on Lake Macbride's beaches Beer and wine — no more than 17 percent alcohol — is allowed in the park's campgrounds said a recent collection of public input and citation data found 'a myriad of concerns' of issues — particularly concerning alcohol use — on a handful of state beaches 'We have a couple areas that have a proclivity for abnormal behavior or unsafe behavior,' he said Coffelt said state committees now are discussing the possibility of banning all alcohol on those beaches The process of reaching a final decision on the matter could take several months 'There's a whole host of steps that have to take place,' he said 'It could take the better part of 180 days to get that done.' All rules pertaining to alcohol consumption on water are defined by state law Operating a boat while over the legal limit — .08 blood alcohol content — can result in a serious misdemeanor boating while intoxicated charge said it has been a challenge in recent years to change the mentality on boating and alcohol 'People just assume that drinking goes with boating 'We've really hit it hard for the past probably five or six years I think people are starting to get the message finally.' but the main focus on safety — particularly when mixing alcohol and water — is uniform across all jurisdictions The Center for Disease Control and Prevention cites alcohol among the factors that increase drowning risk an average of 10 people died each day in non-boat related unintentional drownings An added 332 people died each year from boat-related drownings alcohol use is involved in up to 70 percent of deaths associated with water recreation and about one in every five reported boating deaths 'A lot of our water safety revolves around 'Don't drink and boat and don't drink and swim,'' he said we want to make these family friendly places You can have family friendly activities without alcohol.' The Gazette has been informing Iowans with in-depth local news coverage and insightful analysis for over 140 years independent journalism with a subscription today © 2025 The Gazette | All Rights Reserved Print Leo Kirch who turned his one-man film distribution company into Germany’s second-biggest media business before losing control of it after a gamble on pay television but Kirch had suffered from diabetes and near-blindness for several years At its height, Kirch’s media group was valued at $5 billion It held Germany’s biggest film-licensing library the nation’s only pay-television channel and rights to two World Cup soccer tournaments his companies were under court protection from creditors the biggest bankruptcy filing in Germany since World War II Kirch took time off from teaching economics at Munich University in 1956 He drove to Italy in search of filmmakers and found Federico Fellini who had just directed “La Strada.” Kirch bought the German rights to distribute the movie “La Strada” was popular with German audiences and ultimately considered a classic Kirch kept buying until he had the largest film library outside the United States and the Howard Hughes/RKO library with “King Kong” and “Citizen Kane.” When German Chancellor Helmut Kohl ushered in private television in the 1980s Kirch moved to assemble television properties Kirch began pouring money into a new venture: pay TV In four years he spent more than $3 billion building Premiere World German viewers who already had a wide choice of channels didn’t feel the need to pay for what Premiere World had to offer an acclaimed choreographer whose creations dazzled audiences from Paris to Hollywood Petit took his first dance steps at 9 at the Paris Opera’s School of Dance While opening several ballet companies in Paris after its liberation from the Nazis His reputation grew well beyond France in the 1950s during a four-year stint in Hollywood collaborating with Orson Welles on the 1953 ballet “The Lady in the Ice” and choreographing such film classics as 1952’s “Hans Christian Andersen” with Danny Kaye 1955’s “Daddy Long Legs” with Fred Astaire and Leslie Caron and 1956’s “Anything Goes” with Bing Crosby and Petit’s wife Petit choreographed for Rudolf Nureyev and Margot Fonteyn during an eclectic career with the Paris Opera Casino de Paris and what is now known as National Ballet of Marseille-Roland Petit he began traveling the world to create new ballets or mount old works with the likes of the San Francisco Ballet the Asami Maki Ballet of Tokyo and the National Ballet of China Cal Montney, 91, a longtime staff photographer for the Los Angeles Times whose assignments included the Watts riots died July 4 of congestive heart failure at his home in San Jacinto Montney spent more than 30 years at The Times and worked before that at the Los Angeles Mirror “He had a full range of talents,” said former Times photographer Rick Meyer “He could shoot a fire one day and visit British royalty the next.” and became interested in photography in high school He came to California with his wife and daughter in 1940 then served in the Navy in Texas and Florida from 1944 to 1946 a veteran television announcer for “What’s My Line?” and other game shows died July 9 at a North Hollywood convalescent hospital of complications from a fall and pneumonia Besides appearing on “What’s My Line?” in the 1950s he also was the announcer for “Hallmark Hall of Fame,” “The Name’s the Same,” “Password All-Stars” and other TV programs He had occasional acting roles on TV including on “The Mary Tyler Moore Show.” British actress known for ‘The Lady Vanishes’ a British actress best known for her role in Alfred Hitchcock’s “The Lady Vanishes,” died Friday at her home in Sydney Born Georgette Lizette Withers in what was then British India she was given her nickname by her Indian nanny She was a dancer in a West End production in London when she was offered work in 1935 as a film extra in “The Girl in the Crowd.” Withers appeared in dozens of films in the 1930s, ‘40s and ‘50s, including her role as Blanche in “The Lady Vanishes” playing opposite Margaret Lockwood and Michael Redgrave Among her other films were “On Approval,” “It Always Rains on Sunday” and “Night and the City.” Withers moved to Australia with her husband Her last role was in the 1996 Australian movie “Shine.” a character actor who took the stage name of Billy Beck and appeared in such movies as Billy Wilder’s “Irma la Douce” and “The Fortune Cookie” in the 1960s and 2005’s “Just Like Heaven” with Reese Witherspoon and a slew of TV roles from the 1950s to the 2000s died of congestive heart failure June 29 at a Glendale nursing home news.obits@latimes.com Television Sports Entertainment & Arts Obituaries Sports Entertainment & Arts World & Nation Music Subscribe for unlimited accessSite Map Die Gäste waren abgezockter und cleverer im Abschluss Anna Hettwer und Maren Schulte konnten ihre Chancen nicht verwerten Auf der anderen Seite traf Pakize Gözde Dökel in der 27 Minute mit einem flott vorgetragenen Konter erzielt hatte Ihre Gegenspielerinnen kam im Billerbecker Sechzehner zu Fall Schiri Ümit Ekinci zeigte Rot wegen Notbremse und gab Elfmeter FFC Recklinghausen 0:4; Tore: 0:1 Pakize Gözde Dökel (27.)