Please select what you would like included for printing: Copy the text below and then paste that into your favorite email application the daughter of Arnold and Vivian (Marquart) Breitenfeld Ellen graduated from Horace Mann High School in North Fond du Lac in 1980 After graduation Ellen participated in the Wisconsin Special Olympics in soccer and won a bronze medal Arnold Breitenfeld and his wife Dorothy; her sister Joyce Breitenfeld; and many cousins and friends Vivian Breitenfeld; her grandparents and aunts and uncles from 11:00 AM until 1:00 PM at Uecker-Witt Funeral Home A funeral service will follow the visitation at the funeral home at 1:00 PM Interment will take place at a later date at Estabrooks Cemetery A special thank you to Kelly Kohlmann for being a friend to Ellen and her sister Also special thanks to Lakeland Care District and to Lori from Generations Hospice for everything they have done Services are in the care of Uecker-Witt Funeral Home Additional information and guestbook can be found at ueckerwitt.com Enter your phone number above to have directions sent via text This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply Service map data © OpenStreetMap contributors Breitenfeld has realized the value of significant digitalization but also recognizes the increased cyber security risks that this transformation inevitably carries the need for digitalization on the store floor is increasing so the risk of production downtime due to ransomware or other attacks is also increasing," said Simon Pucher To protect the integrity and availability of its systems the organization needed to safeguard its production systems from cyber disruption Darktrace's Self-Learning AI supports the company's interests in visibility and detection of the most advanced threats Darktrace continuously learns the 'patterns of life' for the entire ecosystem and identifies subtle deviations indicative of a threat Breitenfeld trialed Darktrace's Industrial Immune System in 2017 and went directly from the Proof of Value (POV) period to contract "The POV setup was extremely fast and smooth It was only a few hours before we were seeing results," noted Pucher Breitenfeld recognizes that machine-speed attacks need to be met with machine-speed defense "AI fights against AI"" said Pucher "You can already see today that the majority of attacks are already fully automated so the defense must also be fully automated."When the time came to renew the contract 2020 Breitenfeld did a wider evaluation of their security products Pucher explained that ultimately it was their "high satisfaction with Darktrace as well as the ease of use" that naturally persuaded them to renew the contract Darktrace's ability to learn 'on the job' has helped the company identify threats in their earliest stages Breitenfeld has been able to detect anomalous activity before it has the opportunity to escalate into a crisis Darktrace proved critical in providing greater visibility for Breitenfeld early on when Breitenfeld was experiencing problems after an antivirus update Darktrace's Threat Visualizer allowed Breitenfeld to surface insights across their entire digital infrastructure and see the issue in detail "We activated an antivirus update on all clients which also had the impact of partially disabling TLS protocols We immediately detected an increase in TLS connection problems in the Advanced Search and were able to solve the problem within minutes before the update was rolled out to all clients This also prevented the network from being blocked and our internal customers from getting a bad experience" explained Pucher Breitenfeld no longer lacks visibility across their organization Darktrace illuminated the company's entire digital ecosystem and the complex relationships between them network traffic and associated visibility was a 'black hole.' Basically Now our network traffic is totally transparent and also secured by an additional pair of eyes from the SOC," commented Pucher "Security can only be optimized if you have transparency we are able to have transparency with minimal alerts."Seeing the effectiveness and accuracy of Self-Learning AI Breitenfeld has decided to upgrade to Antigena Darktrace's Autonomous Response technology that neutralizes fast-moving and unpredictable attacks in seconds Pucher explained: "We have been working with the system in order to now have the knowledge that everything fits and have taken the logical step for the activation of Antigena." Recognized that increased digitalization of infrastructure brings new cyber security risks Implemented Darktrace across the entire digital infrastructure Developed trust in AI detections and decision-making; now upgrading to Autonomous Response Sign up to receive the latest news and insights from the Darktrace newsletter – delivered directly to your inbox Darktrace is committed to protecting and respecting your privacy and we’ll only use your personal information to administer your account and to provide the products and services you requested from us we would like to contact you about our products and services as well as other content that may be of interest to you If you consent to us contacting you for this purpose please tick below to say how you would like us to contact you: you consent to allow Darktrace to store and process the personal information submitted above to provide you the content requested Check out this customer story from Darktrace: Breitenfeld: Darktrace AI Success Story As Missouri schools begin preparing for another year of student transfers the woman who brought the case all the way to the state Supreme Court is at the brink of bankruptcy and wondering where her daughters will get their education this fall Gina Breitenfeld is being sued by the Clayton School District for more than $24,000 in unpaid tuition She says that the financial toll of the case plus unpleasant comments about the transfers made within earshot of her daughters prompted her to pull them out of the Clayton schools toward the end of the just completed school year She also had to give up the Clayton apartment she had rented to establish residency so that Elle could continue in the district where they had attended class for several years as the transfer case wound its way through the courts Breitenfeld said in an interview Wednesday she is proud of what the case has brought and doesn’t regret anything that her family has gone through and in the beginning I had no idea it would take this long to come to a conclusion or solve the education problem I feel good about what we’ve accomplished as far as winning in the Missouri Supreme Court I can’t take advantage of that decision right now with my children.” a Saint Louis University professor married to a judge in St But by the time the case reached the Missouri Supreme Court for the second time, Turner’s sons had graduated and gone off to college. So the unanimous opinion that was issued on June 11 upholding the law and setting the transfer scramble in motion was handed down in what had become the Breitenfeld case which prompted both Turner and Breitenfeld to seek to take advantage of the transfer law the city schools had regained provisional accreditation meaning the law no longer applied to students living in the city Breitenfeld said that her daughters had begun attending Clayton schools when Savannah was in kindergarten and Elle was in second grade She is in legal talks to settle the debt for tuition for her daughters for the portion of the 2012-13 school year when the law no longer applied to them she and her children leased an apartment at Clayton on the Park for $1,950 a month to establish residency in the district plus the mortgage on the family’s home in St Louis Hills – what Breitenfeld said totaled more than $5,000 a month – became too much and she’s in the process of completing a bankruptcy filing “We just couldn’t do it,” she said of the financial load she took Savannah and Elle out of the Clayton schools toward the end of the year and home-schooled them She is still trying to figure out where they will be learning when the new school year begins Breitenfeld also talked of the burden on her divided family while her daughters went to school in Clayton so he had to keep living there while she and her daughters moved to the apartment “being in Clayton with the kids without my husband He would come in for dinner once in a while during the week He would help with  the girls’ homework and things of that nature and it was just really hard on our family being apart.” Elle and Savannah have not experienced any adverse reaction about their family’s role in the transfer case But once details became public about the efforts by Clayton to obtain back tuition payments “then there were a couple of comments made to the children or within their hearing distance my husband and I decided we don’t want to subject them to any negativity so let’s just take them out of the school.” Has the case had any lasting effect on her daughters “It’s hard to determine how much a child is affected,” Breitenfeld said “I would say that once in a while they would make a comment that they didn’t want to go to school “It definitely affected my husband and me because we didn’t want them subjected to any negative comments or in any way be the cause of a problem as the legal and educational uncertainty surrounding the transfers continues Breitenfeld insists that the lengthy journey has been worth it for her family and for others who have been able to take advantage of the option to attend an accredited school “We knew all along that the city schools were not where we wanted our children,” she said “Once we were educated and learned what we could do to change things We paid high taxes in the city on our home We had no options for our children as far as a good education without going to a private or parochial school and paying that tuition let’s get them into a good school system where they can get the education that they deserve…. “I definitely have not stepped into anything reluctantly I have from the very beginning felt very strongly about what we are doing Jane’s children graduated and had to pay tuition through graduation day and were not able to take advantage of the things she fought for This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page passed away after a short illness on Sunday 2024 at Rose Senior Living where she resided for over two years.  to Karl Hermann Breitenfeld and Dora Emma Frenzl Breitenfeld They together raised her and her four brothers; Marvin (predeceased along with his wife Richard (Judy) and Robert (predeceased wife Pearl and Herman (predeceased) married on September 30 and traveled a great deal as Herman was a first baseman for the Milwaukee Braves Eric John (predeceased) and Peter Ross.  who iis in his final year of law school at IU McKinney Nick and his grandma spent countless hours talking sports and politics.  Pearl was a practicing Catholic and had attended Our Lady of Mt She also enjoyed her grandson's church Pearl loved to exercise and play golf and even when she couldn't play golf she played tennis at the Carmel Racquet Club regularly She was an avid reader until her eyesight no longer permitted an spent countless hours at the library Vincent North's hospital gift shop delivering flowers and moving patients Pearl loved to laugh with her friends and family.  please send any donations to Tunnel to Towers Foundation Jason Breitenfeld remembers the wispy miniature whirlwinds of snow crossing the road in front of his headlights He remembers the lights of the oncoming car as it swerved into his lane He remembers the helicopter ride from Woodruff to Ministry Saint Joseph’s Hospital in Marshfield “It was one of the coldest rides I ever experienced in my life,” he said “I couldn’t get warm in there.” It was past 2 a.m and Breitenfeld had lost nearly a gallon of blood Breitenfeld had been driving a cab for a company called Star Gazer Ride that Jan It was the week of the World Championship Snowmobile Derby in Eagle River and he was driving as a part of the state’s SafeRide program The drunken driver who crashed head-on into Breitenfeld’s cab The list of injuries Breitenfeld suffered in the crash is ridiculously long: shattered pelvis; left femur broken at the ball of the hip; four compound fractures in his right leg; both feet broken; left hip socket shattered His vehicle was mangled; it took EMTs nearly an hour to extract him today with the help of a cane he can walk — something his doctors told him he would never do But that night changed Breitenfeld’s life irrevocably. On his website he makes a somewhat dark joke about losing his “man card,” for example the ability to be a breadwinner for his wife and two stepsons Besides the limp and the lingering physical pain he also deals with post-traumatic stress disorder and anxiety But he’s also picked up a determination to tell his story and to serve as an advocate for changes to Wisconsin’s drunken driving laws and to try to reach out to those who need to hear about the real risks of driving drunk from someone who has borne the burden of another man’s decision to drink and drive has written a book about his experience: “The Dangers of Drinking and Driving: The Jason Breitenfeld Story,” that he will self-publish in coming months He works with Northwoods Restorative Justice a Woodruff-based nonprofit whose stated mission is to “acknowledge the impact of crime upon victims hold offenders responsible for their actions On Tuesday, Breitenfeld will speak in Wausau about his experience as part of a day-long summit on substance abuse and impaired driving at Northcentral Technical College that will be broadcast online He said his priority is "sharing my story and getting it out there whether that's speaking to offenders or groups of high school kids it’s all about preventing even one family or individual (from having) to endure what my family has gone through." has had: He is not the only person whose life was changed that night for the worse The man who swerved into Breitenfeld’s 2000 Chevy Venture that night outside of St a 38-year-old Bowler man with three previous drunken driving convictions already on his record He would plead no-contest to a fourth OWI in the crash He was sentenced to three years in prison and three years on probation "One of the things that really struck me: He had young kids," Breitenfeld said but he has young kids who have lost their father for at least three years when they hauled him off (after the sentencing) and his kids were crying." Doxtator at the sentencing said he had quit drinking that he understood the consequences of drinking and driving That's what Breitenfeld hopes others can take from his story "but I do believe there’s some hope out there." Robert Mentzer is storytelling coach and a columnist for Gannett Central Wisconsin Media. Contact: robert.mentzer@gannettwisconsin.com, 715-845-0604; on Twitter: @robertmentzer Register online: https://faucetofhope.leadpages.co/teleseminardec8thdui/  The online summit is free; donations are encouraged contact Linda Larson Schlitz at 715-212-2339 Visit www.jasonbreitenfeld.com for more information or to contact Jason Fraud and Identity Solutions Keir Breitenfeld is a Vice President of Product Management and Marketing Fraud and Identity Solutions as part of Experian�s Decision Analytics business unit His responsibilities include stewardship of Experian�s comprehensive suite of consumer and commercial authentication and fraud management products and services.�Keir has been with Experian for 9 years and brings with him a diverse set of experiences including fraud operations management and marketing.� His background prior to joining Experian in 2005 includes serving as a director of product management with both HNC Software and Fair Isaac Corporation and leading fraud detection and prevention units at Fleet/Advanta Credit Card Services and Capital One Financial Corporation.��Prior to entering the financial services arena Navy as a surface warfare officer aboard the guided missile frigate USS Estocin (FFG-15).� He holds an MBA Degree from Duke University�s Fuqua School of Business and a BS Degree in Criminology from Auburn University Make sure you're getting it all Accounting Today is a leading provider of online business news for the accounting community Doctoral students at Boston University could be forced to take a leave of absence and lose their health insurance if they do not comply with new guidelines requiring them to return to campus in the fall mandate that “continuing students with teaching service appointments for fall 2020 who are unable to return to Boston University by September 1 should be expected to take a leave of absence,” according to a memo released last week by University Provost Jean Morrison and Associate Provost for Graduate Affairs Daniel Kleinman at the discretion of the program and school/college the student may attend classes remotely without stipend support.” PhD student Lena Brucia Breitenfeld now has to make a decision: come to campus in-person and continue work as a teaching fellow “This policy is threatening to leverage people's insurance coverage against them and forcing them to choose between their safety and their health care coverage,” she said really dangerous to make people choose between life saving health care and their safety.” this could mean a choice between continuing to have access to life-affirming hormones and other medical needs or giving up health insurance and exposing herself to the public during a pandemic she has until August 1 to let her program know if she will be returning which she said could affect her housing situation too “A couple of my possible living situations are to live with a friend of mine who is immunocompromised one of whom is a senior citizen,” Breitenfeld said “I don't know if it's going to be safe at all for me to go in and thus expose members of my family and my friends.” “Many of the service responsibilities of PhD students particularly those with teaching service appointments cannot be readily done remotely,” the post reads “BU cannot provide stipends to students who are unable to complete their service responsibilities.” a spokesperson for the university said there are some exceptions: namely if a student is able to enroll in courses remotely the university will continue to cover their health insurance but not their salary “BU cannot pay PhD students who are unable to do their 'service;' generally PhD students must be in the Boston area and able to be on campus in order to complete their service responsibilities,” the spokesperson said we expect PhD students to be on campus to receive stipends… For a PhD student who cannot return to campus in the fall and who believes they can effectively do service work off campus they may contact their program director of director of graduate studies to discuss what options are available.” a PhD student in the university’s Classics department and has applied for a “workplace readjustment” in the hopes that she will be able to continue her work remotely as an exception to the new rule “If I'm not granted it and I have to go into campus I would probably have to do that,” she said “in order to keep myself insured and have the ability to buy insulin and other medical supplies I need.” The policy also means international students who are unable to travel to campus by September will also forgo their insurance and salaries tax laws and payroll surcharges in both the United States and within a student’s home country make it financially and operationally infeasible to provide stipend support to international PhD students who are not able to come to the U.S.,” the school’s website reads “There are additional regulatory complexities with respect to paying new international students who are unable to come to the U.S a doctoral student in Education and Human Development said he’s particularly concerned about the impact this policy will have on Black and Indigenous people of color who have been disproportionately affected by COVID-19 “This policy is forcing these students to choose between being able to financially survive and have medical insurance during a global pandemic and protecting their physical well-being against a disease that disproportionately affects these underserved communities," he said “This is not ethical to force us to make that choice,” Chandler-Campbell continued “BU talks a great deal about supporting us — we literally want them to put their money where their mouth is.” said she finds the communication around the policy to be vague and confusing “They haven’t answered questions about how proper ventilation in classrooms will be provided are they just expecting us to trade off duties I don't relish the idea of having to go back to a classroom especially when the administration isn't providing personal protective equipment to the students or to the teaching staff.” Students and teachers will be expected to wear face coverings while on campus and required to provide their own personal protective equipment “They've specifically refused to provide PhD students who are working as staff with any type of PPE,” he said “And what am I going to do if a student forgets to or refuses to wear a mask?” not just for the PhD students returning to campus but for the larger community surrounding the school staff and students at Boston University at risk it's also putting the general community of Boston at risk because the university is asking us to go out into the world,” she said “This isn't just a concern for people in the Boston University community It's it's a concern of everybody that lives nearby.” CORRECTION: An earlier version of this article stated that the policy applies to graduate students Uncategorized Savannah and Elle) was one of the parents on the list Get news and recommendations from the local arts scene sent to your inbox weekly Start your mornings with a fresh take on the day’s top local news from Sarah Fenske and Ryan Krull Our award-winning content brings readers revealing profiles Start your mornings with a fresh take on the day’s top local news BISMARCK — Gary and Donna Ausley and their grandson and his wife are the Hot Spring County Farm Family of the Year This document may not be reprinted without the express written permission of Arkansas Democrat-Gazette Material from the Associated Press is Copyright © 2025 audio and/or video material shall not be published rewritten for broadcast or publication or redistributed directly or indirectly in any medium Neither these AP materials nor any portion thereof may be stored in a computer except for personal and noncommercial use The AP will not be held liable for any delays errors or omissions therefrom or in the transmission or delivery of all or any part thereof or for any damages arising from any of the foregoing Get our local education coverage delivered directly to your inbox E-edition PLUS unlimited articles & videos Personalized news alerts with our mobile app *Refers to the latest 2 years of stltoday.com stories Please subscribe to continue reading… Gina Breitenfeld, who has two children in Clayton schools, is the lone plaintiff in Turner vs. School District of Clayton. She's shown in St. Louis County Circuit Court as the case was argued on March 5, 2012. Breitenfeld has two children in Clayton Schools. Photo by Robert Cohen, rcohen@post-dispatch.com Louis mother whose lawsuit triggered the mass exodus of children from two failing districts is now fighting a different court battle that could draw St Louis Public Schools into the school transfer saga In June, Gina Breitenfeld prevailed in a landmark Missouri Supreme Court case that affirmed the right of children like hers to attend schools in higher-performing districts at no cost to their parents more than 2,000 students in the unaccredited school districts of Normandy and Riverview Gardens have transferred Now Breitenfeld and her attorney argue the law should also apply to students in provisionally accredited school districts such as St “It’s something the Supreme Court should consider,” said her attorney Breitenfeld’s dispute focuses on unpaid tuition payments for her two children Louis but attended Clayton schools for four years Clayton officials are trying to collect about $102,000 in Breitenfeld’s unpaid tuition from the St Louis district for three of the years when city schools were unaccredited They’re also trying to collect $24,000 in tuition from Breitenfeld herself for part of 2012-13 Louis schools’ accreditation status was bumped up to provisional Louis Public Schools are getting out their checkbooks their attorneys are scheduled to fight Clayton’s collection attempts before St Louis County Judge David Lee Vincent III on May 27 Both are raising questions related to the law such as how receiving districts calculate tuition and whether “provisional accreditation” is the legal equivalent of “accredited” in the state’s school rating system Attorneys for city schools accuse the Clayton district of charging “inflated amounts of tuition far above the amounts it contracted to receive from Gina Breitenfeld.” They also argue the St Louis district is not responsible for tuition because the Breitenfeld children did not enroll in Clayton schools under the transfer statute Kistner argues that she shouldn’t be billed for any tuition because city schools are not fully accredited — and the law doesn’t implicitly give relief to districts with provisional accreditation status The law states that any district that “does not maintain an accredited school” must pay the tuition and transportation costs of resident children who wish to transfer a footnote says the school transfer law became inapplicable to St Louis Public Schools the date it gained provisional accreditation Clayton officials say this reference couldn’t be more clear “I’m not sure what higher authority of state law you can get than the Missouri Supreme Court,” said Chris Tennill The dispute over unpaid tuition is a continuation of the six-year court fight that began as Turner vs After the case went to the Supreme Court twice the high court sent one part of it back to St Louis County Circuit Court — the part to determine what St Louis Public Schools would be billed for back tuition payments for the two Breitenfeld children Tennill indicated district officials gave no consideration to walking away from the unpaid bills “We’ve always held all along we would come back and clean up the tuition issues with St Louis Public Schools and the clients that were left in the case,” he said Email notifications are only sent once a day The Missouri Board of Education votes to extend the panel But data suggest families in districts like Normandy who have opted for transfers likely have more resources than those who did not One has to fight like mad to keep ridiculously ill-conceived practices and policies from penetrating classrooms Regarding “Battle over school choice looms over Legislature” (April 28): District accuses receiving districts of charging excessive tuition and the Legislature failed to fix the situation A lawsuit seeking unpaid tuition against the mother in the lawsuit behind the student transfer law has been dismissed -- for now Louis Post-Dispatch articles on the transfer issue Get up-to-the-minute news sent straight to your device That's what more than 1,000 Fort Bragg soldiers learned recently as they jumped under the direction of German soldiers Operation Federal Eagle is an annual exercise that builds partnerships and promotes friendships between the German army's airborne forces and Fort Bragg the airborne exercise is an opportunity for local paratroopers to earn coveted foreign jump wings But it's also helping the 82nd Airborne Division achieve its goal of bridging the gaps between friendly paratroopers around the globe Federal Eagle is one of more than 30 international exercises visits or exchanges conducted in the past 12 months according to a spokesman for the 82nd Airborne including the weeks-long training with British forces earlier this year the foreign engagements have involved at least seven allies They are building toward a huge airborne operation to be led by Fort Bragg forces in Germany later this year approximately 1,200 paratroopers got a taste of Germany over the course of the two-day exercise Federal Eagle would take place in German C-160s The 82nd Combat Aviation Brigade ferried dozens of soldiers over the drop zone letting the paratroopers loose one at a time with the familiar scene of canopies in the sky following German jumpmasters shouted commands that would have been familiar were it not for the language "The result is the same," Breitenfeld said you jump out and you try to keep your legs together." Paratroopers said even the German jumpmaster hand signals were a little different "We're getting exposed to the way they conduct business in the air," said 2nd Lt a paratrooper with the 82nd's 1st Brigade Combat Team That could come in handy should the two nation's paratroopers need to work together you may be less comfortable now," Jenson said it's understanding the capability of our allies and them understanding our capabilities." "It's important for us to exchange our procedures and learn from each other." they pinned the German jump wings to the chests of the paratroopers While not all of the paratroopers jumping will attend the Swift Response exercise later this year Breitenfeld said he looked forward to seeing them all the same The 82nd Airborne Division is popular in Germany His paratroopers look up to the All Americans and respect their expertise Swift Response will involve Fort Bragg's 18th Airborne Corps and the Europe-based 173rd Airborne Brigade Combat Team At least seven nations will participate in the exercise which will involve parachuting into southern Germany with a large international force Perhaps to help those attending that future training Breitenfeld ended each short ceremony with a language lesson of sorts He taught the paratroopers the German version of the familiar "Airborne!" response that is ubiquitous among American paratroopers: "Glück ab!" Military editor Drew Brooks can be reached at brooksd@fayobserver.com or 486-3567 Letter: They don't fix the vendor problem because they don't careNorthwest Florida Daily NewsFor those who continue to wonder why the Destin City Council persists in allowing beach vendors to take over the beaches the answer is very simple — they just don't care however — The Destin City Council does not care Metrics details thermal waters (TW) are all-natural mineral waters that emerge inside a thermal resort and have therapeutic applications Their beneficial effect has been empirically recognized for centuries being indicated for symptom alleviation and/or treatment of several diseases an anti-inflammatory effect has been attributed to many different Portuguese TW but there is no scientific validation supporting this empiric knowledge we aimed to investigate the anti-inflammatory properties of 14 TW pertaining to thermal centers located in the Central Region of Portugal and grouped according to their ionic profile Mouse macrophage cells stimulated with lipopolysaccharide (LPS) were exposed to culture medium prepared in TW inducible nitric oxide synthase (iNOS) expression levels and the scavenging capacity of TW 11 out of 14 TW reduced NO production and/or iNOS expression The sulphated/calcic TW did not show any effect on at least one of the inflammatory parameters evaluated Two sulphurous/bicarbonate/sodic TW and the sulphurous/chlorinated/sodic TW promoted an increase in NO production and/or iNOS expression the anti-inflammatory properties of Portuguese TW supporting their therapeutic use in the treatment of inflammation-related diseases and promoting their putative application in cosmetic products and medical devices described in detail in Supplementary data online the respective main chemical composition and general therapeutic indications These diseases are associated with an overproduction and release of proinflammatory mediators by macrophages during inflammation biological scientific data supporting the health benefits of Portuguese TW use remain insufficient the present study aimed to evaluate the anti-inflammatory properties of these waters in vitro we analyzed key inflammatory parameters in a mouse macrophage cell line stimulated with LPS NO production and expression levels of iNOS and the scavenging capacity of TW in removing NO Our work showed that among the 14 TW used in this study and five exhibited scavenging activity in LPS-exposed macrophages supporting the anti-inflammatory properties of Portuguese TW Thermal waters effect on macrophages metabolic state Cells were plated and exposed to Ctrl medium or SBS (a) BMF (e) and SC (f) thermal waters (TW) for 24 h Alamar blue assay was further performed to assess cell metabolism Data correspond to the means ± SEM of at least three independent experiments and are represented as % of control cells (Ctrl Statistical analysis: Unpaired t-test; p < 0.05 was considered significant: * p < 0.05 SBSF sulphurous/bicarbonate/sodic/fluoridated suggesting that cells might need an adaptation period to these TW Thermal waters effect on macrophage NO production upon an inflammatory stimulus in the presence or absence of LPS (1 µg/mL) Griess assay was performed to assess nitrites levels in the supernatant Data correspond to the means ± SEM of at least three independent experiments and are represented as % of control (Ctrl) cells exposed to LPS (Ctrl LPS) Statistical analysis: two-way ANOVA with Tukey’s multiple comparison test; p < 0.05 was considered significant: * p < 0.05 Thermal waters effect on iNOS expression levels of macrophages exposed to an inflammatory stimulus iNOS and tubulin (Tub) expression levels were determined by Western blotting iNOS levels were normalized to the loading control Tub Representative images of western-blots (cropped from different parts of the same/or different gels) are shown Full-length blots/gels are presented in the supplementary data 2—“Supplementary blots” 300 µM) was added to Ctrl medium or medium prepared with SBS (a) Data correspond to the means ± SEM of at least three independent experiments and are represented as % of Ctrl medium with SNAP (Ctrl) The present study was aimed at conferring scientific validation to the empirically recognized anti-inflammatory effects of Portuguese TW the anti-inflammatory properties of TW from 14 Thermal Centers located in the Central Region of Portugal were validated iNOS expression levels and the NO scavenging capacity of TW were investigated in an in vitro model of inflammation consisting of a mouse macrophage cell line stimulated with the Toll-like receptor 4 agonist 11 promoted a reduction in NO production and/or iNOS expression and/or exhibited NO scavenging activity in macrophages exposed to LPS supporting their anti-inflammatory properties supporting the use of this TW in regenerative skin processes This also could explain the down-regulation of iNOS in cells exposed to SBS TW 1 the results suggest that these TW hold strong anti-inflammatory properties and should be indicated for the treatment of inflammatory diseases It is important to emphasize that the present study focused on relevant but not all inflammation-related mediators and molecular events hence not disclosing the existence of others being affected by TW exposure The results herein presented intended to scientifically justify the anti-inflammatory effect attributable to Portuguese TW without scrutinize the associated mechanisms a deeper investigation about the molecular and cellular pathways associated to TW anti-inflammatory properties using other cell lines and more physiologic cellular models (e.g based on a methodology specifically designed to the study of a particular disease one cannot exclude other factors contributing to TW effects our results greatly contributed to the scientific validation of the anti-inflammatory properties of Portuguese TW supporting their therapeutic use for the treatment of inflammatory-related diseases and further application in cosmetic products and medical devices Water containers were purchased from VWR (Alfragide Raw 264.7 cell line was acquired from American Type Culture Collection (ATCC-TIB-71) and kindly supplied by Dr Otília Vieira (Center for Neuroscience and Cell Biology Dulbecco's Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were purchased from Thermo Fisher Scientific (Massachusetts LPS from Escherichia coli (serotype 026:B6) was obtained from Sigma-Aldrich Corp The protease and phosphatase inhibitor cocktails were obtained from Roche (Mannheim Acrylamide was purchased from Promega (Madison USA) and the Polyvinylidene difluoride (PVDF) membranes were from Millipore Corp The primary antibody mouse anti-iNOS was from R&D Systems (Minneapolis USA) and mouse anti-β-Tubulin was obtained from Sigma-Aldrich Corp The anti-mouse Horseradish Peroxidase conjugated secondary antibody was purchased from Santa Cruz Biotechnology (Heidelberg Enhanced chemiluminescence (ECL) substrate was obtained from Bio-Rad (Hercules All other reagents were purchased from Sigma-Aldrich Corp a mouse leukaemic monocyte macrophage cell line low glucose with pyruvate and L-Glutamine) supplemented with 10% (v/v) of non-inactivated FBS 100 U/ml penicillin and 100 μg/ml streptomycin D-Glucose (up to the final concentration of 4.5 g/L) and sodium bicarbonate (1.5 g/L) at 37 °C in a humidified atmosphere of 95% air and 5% CO2 the cells were passaged using a cell scrapper to detach the cells which were further subcultured in fresh culture media according to ATCC recommendations To determine the effects of each TW on cells culture medium was prepared using TW instead of ultrapure water (control) After the culture medium components were dissolved the medium was filtered with a sterile 0.2 μm membrane TW-containing media was prepared from a newly opened flask each time needed cell duplicates were plated in a density of 2.50 × 105 cells/mL in a 96-well plate with a final volume of 0.2 mL/well 50 µM of resazurin solution (in sterile PBS) was added to each well for 4 h Absorbance was read at 570 and 620 nm with a Synergy HT multi‐mode microplate reader (BioTek Metabolically active cells reduce resazurin (a non-fluorescent blue dye) into resorufin (pink colored and fluorescent form) and their number correlates with the magnitude of dye reduction Results were expressed as a percentage of control Cells were plated at a density of 2.50 × 105 cells/mL in a 6-well plate in a final volume of 2 mL/well Cells were detached using a scrapper and 0.01 mL of 0.4% (w/v) trypan blue stock solution in PBS was added (1:1 ratio) The number of viable cells was counted manually using a Neubauer chamber equal volumes of cell culture supernatants and Griess reagent [1% (w/v) sulphanilamide in 5% (w/v) phosphoric acid and 0.1% (w/v) N-(1-naphthyl)-ethylenediamine dihydrochloride] were mixed and incubated at room temperature (RT) for 30 min The absorbance was measured at 550 nm in a Synergy HT multi‐mode microplate reader (BioTek Nitrite concentration was calculated through a regression analysis of a sodium nitrite standard curve Cells were lysed with RIPA buffer (150 mM NaCl; 50 mM Tris–HCl pH 8.0; 1% Nonidet P-40; 0.5% v/v sodium deoxycholate; 0.1% v/v SDS; 2 mM EDTA) freshly supplemented with DTT (1 mM) and protease and phosphatase inhibitor cocktails the cells were centrifuged (12,000 g for 10 min at 4 °C) and protein concentration was calculated using the bicinchoninic acid method for 5 min (in sample buffer: 0.125 mM Tris pH 6.8; 2% SDS (w/v); 100 mM DTT; 10% glycerol (v/v) and bromophenol blue) 30 µg of protein were separated on a 10% (v/v) sodium dodecyl sulfate (SDS) polyacrylamide gel and transferred to a PVDF membrane Membranes were further blocked with 5% (w/v) fat-free dry milk in TBS-T (Tris-buffered saline/0.1% Tween 20 (v/v)) at RT and incubated O/N at 4 °C with the primary antibody anti-iNOS (1:1000) the membranes were incubated with anti-mouse horseradish peroxidase conjugated secondary antibody (1:40,000) Membranes were also incubated with a mouse anti-β-tubulin antibody (1:20,000) as a loading control Blots were visualized by chemiluminescence using ImageQuant LAS 500 (GE Healthcare USA) and analyzed using TotalLab TL120 software NO scavenging activity was evaluated by adding the NO donor S-nitroso-N-acetylpenicillamine (SNAP Nitrite levels in the medium were then quantified by Griess method The results are presented as mean ± SEM of the indicated number of experiments and were analyzed with t-test or two-way ANOVA with Tukey’s multiple comparision test, using GraphPad Prism (GraphPad Software, La Jolla California USA; www.graphpad.com) Directive 2009/54/EC of the European Parliament and of the Council APIAM - Associação Portuguesa dos Industriais de Águas Minerais Naturais e de Nascente Águas Minerais Naturais e Águas de Nascente – Livro Branco Manual de Boas Práticas dos Estabelecimentos Termais Natural mineral waters: Chemical characteristics and health effects Guia de Análise Química das Águas Potáveis Minerais e para a Indústria (Associação dos Estudantes do IST Peloids and pelotherapy: historical evolution Efectos secundarios y respuestas anormales in Técnicas y Tecnologías en Hidrología Médica e Hidroterapia (ed Agencia de Evaluación de Tecnologías Sanitarias Balneotherapy (or spa therapy) for rheumatoid arthritis An abridged version of cochrane systematic review Balneotherapy in rheumatoid arthritis: a systematic review Evaluation of the role of balneotherapy in rehabilitation medicine Biological effects of thermal water-associated hydrogen sulfide on human airways and associated immune cells: implications for respiratory diseases Characterization of Portuguese thermo-mineral waters to be applied in peloids maturation Physicochemical fingerprinting of thermal waters of Beira Interior region of Portugal Os recursos geotérmicos de baixa entalpia em Portugal Continental e seu tipo de aproveitamento Indicações terapêuticas dos estabelecimentos termais portugueses Human disorders associated with inflammation and the evolving role of natural products to overcome water of Nagybaracska on patients with knee joint osteoarthritis—a double blind study Therapeutic effect of spa therapy and short wave therapy in knee osteoarthritis: a randomized Bath thermal waters in the treatment of knee osteoarthritis: a randomized controlled clinical trial Sulphurous mineral waters: new applications for health Afecciones Dermatológicas y Cosmética Dermotermal in Técnicas y Tecnologías en Hidrología Médica e Hidroterapia (ed Quality of Life of Psoriasis Patients before and after Balneo-or Balneophototherapy Thermal waters as cosmeceuticals: La Roche-Posay thermal spring water example Immunomodulation induced by Avène spring water on Th1- and Th2-dependent cytokine production in healthy subjects and atopic dermatitis patients Eau thermale d’Avène et dermatite atopique: Avène’s thermal water and atopic dermatitis Gene and protein expression of CXCR4 in adult and elderly patients with chronic rhinitis pharyngitis or sinusitis undergoing thermal water nasal inhalations Sulphurous water inhalations in the prophylaxis of recurrent upper respiratory tract infections Curas balnearias como agentes terapéuticos Bases biológicas in Técnicas y Tecnologías en Hidrología Médica e Hidroterapia 27–32 (Agencia de Evaluación de Tecnologías Sanitarias Impact of Different Spa Waters on Inflammation Parameters in Human Keratinocyte HaCaT Cells Inhibition of TNF-alpha induced-adhesion molecules by Avène Thermal Spring Water in human endothelial cells Avène thermal spring water: an active component with specific properties Effect of Avène spring water on the activation of rat mast cell by substance P or antigen Inhibitory effect of Avène spring water on vasoactive intestinal peptide-induced inflammation in surviving human skin Effect of São Pedro do Sul thermal water on skin irritation Discrepancies between metabolic activity and DNA content as tool to assess cell proliferation in cancer research In Vitro Cytotoxicity and Cell Viability Assays: Principles and Disadvantages in Genotoxicity – A Predictable Risk to Our Actual World (eds Inducible nitric oxide synthase: Regulation Nitric oxide synthases: regulation and function Nitric oxide therapy for dermatologic disease Sulphurous thermal water increases the release of the anti-inflammatory cytokine IL-10 and modulates antioxidant enzyme activity Chemical signature and antimicrobial activity of Central Portuguese Natural Mineral Waters against selected skin pathogens Hydrogen sulfide modulates the release of nitric oxide and VEGF in human keratinocytes Nitric oxide suppresses inducible nitric oxide synthase expression by inhibiting post-translational modification of IkappaB Aquaphilus dolomiae extract counteracts the effects of cutaneous S aureus secretome isolated from atopic children on CD4+ T cell activation Zinc: an antioxidant and anti-inflammatory agent: role of zinc in degenerative disorders of aging The role of magnesium in different inflammatory diseases Standard Methods for the Examination of Water and Wastewater (American Public Health Association Investigation of the Alamar blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity Respiratory sensitizer hexamethylene diisocyanate inhibits SOD 1 and induces ERK-dependent detoxifying and maturation pathways in dendritic-like cells Download references This work was financed by the European Regional Development Fund (ERDF) through the Centro 2020 Regional Operational Programme and Economic Enhancement Program for Endogenous Resources—3rd phase—Anchor Projects for the Implementation of Collective Efficiency Strategies (PROVERE): project CENTRO-04-3928-FEDER-000010—Valorization of the Thermal resorts of the Centre Region—Characterization of the bioactive potential of the thermal water resources of the Region—"Study of the therapeutic properties of thermal waters—dermocosmetic" We would also like to thank ‘Termas de Portugal’ and all the Portuguese thermal centers that accepted to participate in this study Health Sciences Research Centre (CICS-UBI) Labfit–Health Products Research and Development Lda Centre for Innovative Biomedicine and Biotechnology (CIBB) analysed and interpreted the data and wrote the main manuscript text; A.S.O performed experiments and analysed the data; S.C. contributed as consultants regarding data interpretation; A.P.O The authors declare no competing interests Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Reprints and permissions Download citation DOI: https://doi.org/10.1038/s41598-020-79394-9 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article International Journal of Biometeorology (2025) Environmental Science and Pollution Research (2022) International Journal of Biometeorology (2022) Sign up for the Nature Briefing newsletter — what matters in science Please enable JS and disable any ad blocker Stay up-to-date on the latest in local and national government and political topics with our newsletter Repair technician Jared Rieger works on fixing a radiator hose leak on a 2000 refuse truck at the city vehicle maintenance building on Thursday, Aug. 16, 2018. Fourteen trucks filled the repair bays Thursday, a number that varies as technicians staff the facility six days a week to keep the aging vehicles running. Photo by Robert Cohen, rcohen@post-dispatch.com LOUIS — The city trash commissioner on Wednesday told aldermen the budget they are considering won’t pay for enough truck drivers to pick up all the trash Refuse Commissioner Randy Breitenfeld said in budget hearings that next year’s spending plan includes nine fewer trash truck drivers than the city needs “I would not be able to cover all my routes,” Breitenfeld said The statement set off alarms among aldermen. Trash pickup is one of the city’s bread-and-butter functions, and aldermen have been getting an earful from constituents in the last few years as a short-staffed refuse department has struggled to maintain consistent service “That is a pretty big omission,” said Spencer who as a member of the city's Estimate Board gave the city's entire $1.3 billion budget initial approval last month said in an interview that the part Breitenfeld objects to should probably change “We should be aspiring to have the workforce to pick up every single route every single day,” she said said in an emailed statement that it is important to her that the refuse department has sufficient staff and that she will support budget requests for sufficient staff who also gave initial approval to the budget said he talked to Streets Department Director Betherny Williams on Wednesday assured him that the new budget would have enough drivers to cover all of the routes even if it would be a little tight: The division will have to continue picking up seven days per week instead of the traditional four Breitenfeld described things differently in Wednesday’s committee hearing has been working at full-tilt since the pandemic when staffing got squeezed by a hiring freeze and people leaving for better-paying jobs in the private sector seven-day schedule since at least the summer of 2022 when they restarted recycling pickup and the system collapsed Breitenfeld acknowledged that vacancies remain — there are 52 positions filled out of 66 budgeted for the current year — and said that’s probably why some of the positions were cut The city is trying to be conservative heading into the next fiscal year: Officials are concerned about losing $26 million in income taxes amid challenges from the state and various lawsuits The city doesn’t pay as much as private trash haulers Some applicants can’t pass federally mandated drug tests or don’t show up to interviews But he said he had hoped this would be the year he got the division back to some semblance of normalcy The city has raised salaries for its trash truck drivers and some of those who left have started coming back He said he’d need more than just the 57 positions in the proposed budget for next year and an average of 10 people out each day for medical leave “I have to have some fluff in there,” he said Aldermen will be reviewing the budgets of all city departments over the next few weeks and they can propose additions to the Estimate Board The budget must be finally approved by June 30 Louis through the Post-Dispatch photographers' lenses The mayor announced an end to limits on hiring in city government citing the state Legislature’s inaction on bills that would have cut into c… heavy workloads and poor treatment from management Only about a third of what’s put into recycling bins has actually been processed in recent years The Estimate Board will meet virtually at 2 p.m Louis mayors have been defending the 1% earnings tax since its inception in the 1950s WI -- Spooner Health made history with hospitals around the country by receiving its first supply of the Pfizer COVID-19 vaccine It was delivered for distribution to frontline healthcare workers on December 17 The first recipients of the vaccine include Mark Van Etten “The arrival of the COVID-19 vaccine is a great first step in the timeline of recovering from this pandemic and I could not be more thrilled that the first supply that we received covers all of our frontline workers here at Spooner Health It is only a matter of time before our friends family and community members are also receiving the vaccine and hope beyond this pandemic is achieved.” The impact of COVID-19 for Washburn County and beyond has been huge Healthcare workers faced unknown challenges daily as numbers continued to rise alarmingly high in Washburn County these challenges will not immediately go away with the arrival of the COVID-19 vaccine “We must remain vigilant as a community with continued support of public health policies which include wearing masks avoiding large gatherings and staying home when ill This is an important milestone and together with the arrival of the vaccine there is even greater confidence that this pandemic will end and as a community For more information on the COVID-19 vaccine, please visit www.SpoonerHealth.com/COVID19 Director of Pharmacy; Front L-R: Mark Van Etten Medical Director of Spooner Health; Jill Andrew Submit A Story or Press Release: DrydenWire@gmail.com Advertising QuestionsGeneral QuestionsDrydenWire Insider Questions Science fiction novelist Richard Fox’s second novel in his The Ember War Saga will be receiving a graphic novel adaptation The adaptation is currently being crowdfunded on Kickstarter It is being adapted by comic book writer and fellow novelist Jon Del Arroz with artwork by Oliver Borges who previously worked on Bloodshot: Rising Spirit and Zenescope’s The Courier Liberty and Death Del Arroz previously adapted the first book in the saga RELATED: As Marvel And DC Comics Get Trounced By Manga, Jon Del Arroz Launches Manga-Inspired Crusades Isekai The official description for the series states “An ancient holocaust holds the key to humanity’s survival Only a sliver of mankind survived the Xaros invasion nothing can stop the aliens’ inevitable return.” “Hope arrives from a cryptic message from a long-lost alien race promising the means to rebuild the shattered space fleet Captain Valdar takes one of the last strike carriers and his shell-shocked crew on a desperate mission to the dead world humans aren’t the only power that received the ancient message…” If you are unfamiliar with The Ember War, the official description for the nine book saga on Amazon states “A merciless alien race bent on human extermination has pointed its armada towards Earth The countdown to their arrival begins.” “A mysterious intelligence warns only a young scientist the two create a plan for humanity to survive the invasion Even with the aid of advanced new technology The plan—if it works—can only save a fraction of us,” it continues How can humanity win the battle against almost certain annihilation With what will only be an ember of humanity left will it be enough to rekindle our chance for survival and strike back at the Xaros?” RELATED: 5 Properties That Can Replace Star Wars Fox describes the series as “Battlestar Galactica meets Mass Effect It is a story of first contact with galactic empires others that see us as an infestation to be wiped out.” heroes and villains you’ll never forget and just the right amount of humor to make you bust out laughing while you’re reading in public.” The Ember War: The Ruins of Anthalas graphic novel will feature the option to purchase a variant cover by Joe Bennett The Kickstarter campaign also has a $30,000 stretch goal that includes the adaptation of the short story “Iron Hearts.” The short story was originally published in Ember War Tales alongside “Vent Rats,” “The Anvil,” “Iron Within,” “Schism,” and “Going Dark.”  The official description for “Iron Hearts” states soldier of the Iron Hearts and pilot of a mechanized suit of armor His mind trapped within the prison of his failing body With no other option but to watch their friend wither away his fellow Iron Hearts concoct a dangerous plan to save him.” The campaign also includes the option to purchase the first graphic novel adaptation if you missed out on that campaign. You can also read the first volume digitally on Arkhaven.com As of writing the Kickstarter campaign has 13 backers and has raised $646 Do you plan on backing The Ember War: Ruins of Anthalas NEXT: Graham Nolan’s ‘Giant-Size Two-Fisted Manly Tales’ Promises Multiple Tales Of “Toxic Masculinity” More About: Subscribe to BuzzFeed Daily NewsletterCaret DownAll The Best YA Book Covers Of 2021You are fully welcome to judge books by their cover in this post Moth is searching for something to ground her the two set off on a road trip to find the past and begin to move forward Becca has grown up in a community of doomsday preppers so when a devastating accident rocks her family it's no surprise that many of them retreat farther inward into their doomsday ideology it means her plan to earn a full ride to college and move away is thrown into jeopardy the boy the community was planning to set her up with Xiang is desperate to prove herself to the mother that never has enough time for her She unexpectedly gets a chance at adventure when her pendant the one memento of her father who died at sea before she was born But Anh returns after realizing there is a tiny map scroll hidden inside that she needs Xiang to help decode and the two set off on a journey toward fabled treasure and her father's secrets Shortly after Devon and Chiamaka are selected to be among the senior class prefects at Niveus Private Academy anonymous text messages sent by an unknown figure who goes by the name "Aces" begin going out to students These texts reveal secrets about Devon and Chiamaka's lives — secrets that threaten every aspect of their carefully planned future and the conspiracy turns out to have much deeper roots than either imagined Cover art and design by Natasha Cunningham. Colbert's searing nonfiction debut covers the Tulsa Race Massacre of 1921 seen through the history and development of the area and the attitudes of the people as well as how and what happened and the legacy it left Colbert chronicles one of the most devastating acts of racial violence in US history Cover art by Jenn Manning; cover design by Erin Fitzsimmons and Amy Ryan Marigold’s newly blended family has just moved to the Midwestern city of Cedarville for her mom’s new job — a job that comes complete with a free But there are secrets in their home: Things have been vanishing keeps talking about her friend who wants Mari gone.. 18-year-old Daunis has been dreaming of a fresh start in college since she's never felt like she fit in her hometown or on the nearby Ojibwe reservation a new recruit on her brother's hockey team she's pulled into an FBI investigation of a lethal new drug and ends up undercover to track down the source living in a skydome — supposedly a sanctuary for those committed to planetary protection and the last unpolluted place on Earth Though she's expected to use science to help humanity she can't help thinking of its failure when it came to her sister Though Kasey has never fully believed that her sister is dead as everyone says she doesn't know the truth: Three years prior where she had an old android and a single memory: that of a sister Cover art by Val Wise Antisocial lesbian Annie needs to round out her college applications (and make a few friends BeeBee is trying to keep her parents happy and in the process keep their support of her transition Designed by Baily Crawford Gio has just started to get his life together when his birth mom returns and he hasn't heard from her in eight years Now he's not sure he's willing to let her back into his life there's a new guy on the basketball team that he's been hanging out with and Gio is not sure if he wants to be in a relationship right now The world seems to have ideas about what Charlie Vega should look like, which can make it hard for her to have a good relationship with her body. But Charlie's best friend which is especially important as Charlie's mom keeps passive-aggressively leaving weight loss shakes on her dresser When Charlie starts a relationship with Brian But everything gets messier when she discovers that he asked Amelia out first Cover Illustration by Ryan Garcia; art direction by Kathleen Oudit Wyatt is a witch in a world named Asalin where fae rule is not only Wyatt's best friend but also his betrothed But Wyatt fled to the human world after losing control of his magic...and Emyr has now hunted him to this world Though the now-cold Emyr states that the throne is lost if they don't marry now Wyatt strikes a deal with the enemy in hopes of leaving Asalin behind forever After Liam Cooper's older brother is killed in a hit-and-run Liam feels more alone and isolated than ever It doesn't help that the relationship with their two best friends seems to be fading away But as he starts spending time with Marcus Liam finally finds someone that seems to know exactly what they are going through Sisters Dani and Eden Rivera have taken wildly different approaches to life as dragon slayers While Dani prioritizes her high school normalcy But when Dani comes face to face with a dragon named Nox and forms a rare magical bond with him she comes to realize that everything she's been taught about dragons like the kill-or-be-killed instincts they've been trained to have the ceremony that determines whether she will become a member of her village And Deka knows she will face the consequences But that's before a mysterious woman offers her a choice: Stay in the village to face the backlash or join an army of girls like her — they're called alaki near-immortals with rare gifts — who fight for the emperor And as she journeys to the capital for training she will discover that nothing and no one are quite what they seem to be Amelia and Jenna were brought together by the Orman Chronicles series When the pair get a chance to attend a book fest Endsley will be at what starts as a dream quickly goes wrong: Jenna gets a chance to meet the author But a mysterious rare edition of the Orman Chronicles makes its way to Amelia and she tracks it to a small bookstore in Michigan a Cottonmouth kid from the land of spirits and monsters decide to help each other after catastrophe strikes and collides their worlds together despite those who would kill to keep them apart Agender teen Syd works at the Proud Muffin, a queer bakery and community space in Austin. But after being dumped, Syd's baking, which has always been an outlet for big feelings, quite literally takes on the feelings Syd was trying to bake away: Everyone who eats Syd's breakup brownies breaks up to fix things...including the magical brownie-induced breakup of Vin and Alec Jacket art by Zheng Wei Gu; jacket design and lettering by Catherine Lee Ahn is a peasant girl who accidentally reveals her deadly magic while Altan is a lost heir searching for revenge they see the thing they most want: Ahn sees a path to her past and her magic and Altan sees a path to his future and his throne Jules was going to lay low for the last 10 months of high school in Corpus Christi and discovering that he came out on Twitter between tequila shots Not only does this lead to a DM from Mat (an openly gay Twitter mutual who is supportive cute...and unfortunately three states away) but it also means that he'll have to face coming out to his homophobic father When 17-year-old journalist Josie wins a contest to write a celebrity profile The fact that the subject of the profile is the young up-and-comer Marius Canet certainly contributes to that she ends up face-to-face with a young actor who lets her in on a terrible secret about a Hollywood mainstay leading to more and more accounts from other women Josie has to figure out if she's willing to speak up and expose him and not let down those who entrusted her with their stories the early fears of Mateo and his sister Sophie – that their parents could be deported – come crashing back to the surface Mateo returns from school one day to discover that his parents have been taken by immigration officers and he has to not only take care of Sophie as he waits to learn his parents fate but he must also face questions of his identity in a country that rejects his family Izumi isn't expecting to find out that the father she never knew is actually the Crown Prince of Japan it leads her on a whirlwind trip involving cousins she was never "American enough," and now in Japan she must prove she is "Japanese enough." Will she be able to live out the fairy tale Zetian intends to assassinate the pilot responsible for her sister’s death she offers herself up as a concubine-pilot paired up with a boy of Huaxia to pilot giant transforming robots called Chrysalises that battle the mecha aliens beyond the Great Wall After she murders him and emerges from the cockpit unscathed a female pilot who can sacrifice boys to power up a Chrysalis she will leverage her might to figure out exactly why the pilot system is set up the way that it is who by night is a legendary young mercenary and by day nothing more than bones He'll help train her in exchange for her help breaking his curse finding the death that has been prophesied for him even as she slowly grows to understand (and possibly love) him The March sisters live in the Freedmen’s Colony of Roanoke Island a haven for the recently emancipated in 1863 Jo writes nonfiction about being Black in America and is questioning the stipulations of a publishing offer searching for a purpose and dealing with a mysterious illness wanting to explore life outside their home Jacket Illustration by Raymond Sebastien; jacket design by Jeanette Levy and Jet Purdie Briseis can grow plants from seeds to blooms with a single touch, but she doesn't quite know how to control her gift. When her aunt dies and leaves her a dilapidated, rural New York estate and a garden filled with some of the world's deadliest botanicals Bri discovers that not only does she have a talent for making them but that there is also more to her inheritance than she ever could have imagined Holly Liddell was promised eternal love by Elton Irving Holly is still a 16-year-old (albeit a vampiric But things change when she meets Rose and Ida both also turned into vampires by Elton and then cast away in 1954 and 1921 respectively And they plan to kill Elton and want Holly's help when she meets and begins to fall for a girl named Parker loses control of her forbidden magic on the morning of the betrothal ceremony she never wanted her stepmother Raikama banishes her — turning her brothers into cranes and making it so that every word that escapes Shiori's lips will cost a brother his life she realizes that she has to place her trust in the boy she fought so hard not to marry stuck in a loop where they relive their death over and over again every rule about ghosts is thrown out when Sawyer — who shot and killed 16 kids at a local high school last year before taking his own life — arrives as bodies begin turning up in his neighborhood The Eight Courts of Folk have peacefully lived but there's war brewing between the Mortal and Immortal realms thrown to the forefront after a series of ritualistic murders in Toronto Four queer teens — a half-fae outcast of a royal family and the prince's guardian — are trying to track down the killer