Thanks for visiting
The use of software that blocks ads hinders our ability to serve you the content you came here to enjoy
We ask that you consider turning off your ad blocker so we can deliver you the best experience possible while you are here
Our former tenant and current Associate Member, Dr Christina Lienen, recently published a peer-reviewed article in a Q1 academic journal
titled “French Islamophobia: How Orthopraxy Is Conceptualized as a Public Peril,” addresses issues of equality
focusing on the challenges faced by Muslims in France over the past two decades
policy and executive statements and argues that France’s approach to Islam involves a two-step process of institutionalized Islamophobia
the state redefines mainstream Islamic orthopraxy as “extreme,” positioning ordinary religious practices against averred Republican values
it promotes an alternative concept of “French Islam” that aligns with secular principles and strips the faith of its religious essence
This redefined version is presented as the only acceptable way for Muslims to practice their faith in France
The paper contends that this process is not about upholding laïcité or state neutrality
and provides a critical new perspective on the ongoing debate about religious freedom and secularism in France
She is currently Assistant Professor of Public Law and Head of the Bachelor of Law programme at the British University in Dubai
Get alerts about our latest news & upcoming events
Subscribe
Cornerstone Barristers regulated by the Bar Standards Board.
Website by Square Eye Ltd
After 11 days in Sotogrande in southern Spain
during the which the Boys in Brown continued their preparations for the second half of the season
head coach Ewald Lienen can look back on a positive training camp
"Thanks to the quality of the pitches and the weather we were able to work on a lot of different things in Sotogrande," said Lienen
"We needed to find the right balance between work and recuperation and so as well some high-intensity days with two sessions we also had some rest days
The warm-up matches are incredibly important
We wanted to be fresh going into them and so we reduced the workload accordingly on the day before the game"
And despite the two defeats in the final two games against FC Zurich (1-3) and Dynamo Kyiv (1-3)
Lienen was satisfied with the performances of his team as a whole
"I'm happy with the progress we've made here in Sotogrande," he said just before departing for Germany
After almost two months out with injury (MCL tear in his knee)
Christopher Buchtmann returned to the training ground in Spain
"Buchti's a key player for us," said Lienen
"He was out for a long time but the knee looks stable." Robin Himmelmann (muscle tear) resumed training as well
"He's also well on the way back to fighting fitness," a delighted Lienen added
Ewald Lienen had lots of positives to jot down in his notebook
had to leave Sotogrande early due to illness
"He'd worked so long to get back into the team so I fell really sorry for him," said Lienen of his defender
for whom the Stuttgart game will come too early
Lienen will also be without Aziz Bouhaddouz
Johannes Flum and Mats Møller Dæhli leave him in an optimistic mood: "I think we're well set
We have lots of alternatives and different options now
Who plays will be down to the attitude and physical condition of the players."
Asked whether his charges have the right mentality for the battle against relegation
Lienen said: "I see a squad that is highly motivated and understands what's at stake
They are extremely committed in everything they do
We got back on the right track before Christmas and we've continued along that path during the winter break."
German professional dressage rider Ann-Christin Wienkamp will be leaving Oliver Oelrich's yard
to set up her own independent business at a new base
Wienkamp has been working at Olivier Oelrich's yard in Lengerich for more than 10 years
The 32-year old Wienkamp began her career as a professional rider with the Egbers family in Haftenkamp after which she went to work for Oelrich
She has achieved over 10 medals at World Young Horse Championships and Bundeschampionships and qualified several horses for the Nurnberger Burgpokal finals
is the gold medal at the 2017 World Young Horse championships on Don Martillo
Besides the professional training of dressage horses
New base for Wienkamp at Löffeld yard in Lienen"The yard in Lengerich has simply become too small for two highly asked professionals
I'm very happy that I will not only have the perfect conditions for horse and rider at the newly constructed facility of the Löffeld family
but also that I can continue to collaborate with Oliver by staying close in the area," said Wienkamp
"Also Hans-Heinrich Meyer zu Strohen will continue to help me with the training of the horses."
Oelrich added," in the last few years Ann-Christin built up a big clientele so that our space has reached its limit
I'm very happy that Ann-Chrisin will have the best conditions at the Löffeld yard and we can continue to collaborate."
Stalls for Rent at Durondeau Dressage in Peer, Belgium
Exceptionally Well Located Equestrian Facility in Wellington, Florida
Well-built Equestrian Estate With Multiple Business Opportunities in Sweden
Stable Units for Rent at Lotje Schoots' Equestrian Center in Houten (NED)
For Rent: Several Apartments and Stable Wing at High-End Equestrian Facility
Stable Wing Available at Reiterhof Wensing on Dutch/German border
Real Estate: Well-Appointed Country House with Extensive Equestrian Facility in the U.K.
Rémi Blot
who were announcing the deal for 22-year-old Borussia Dortmund striker Marvin Ducksch
came up with the solution when Lienen missed the press conference
St Pauli then confirmed a body double had been used, tweeting a close up of the mask
The club released a quote from the real Lienen
who said: “With Marvin we get an attacker who has good technique and a very good end product.” Ducksch added: “I am looking forward to the challenge at St Pauli.”
This website is using a security service to protect itself from online attacks
The action you just performed triggered the security solution
There are several actions that could trigger this block including submitting a certain word or phrase
You can email the site owner to let them know you were blocked
Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page
By dallasnews Administrator
Margaret Von Lienen was 5 years old when her father
was shot down while flying an A-4 Skyhawk on a night mission over North Vietnam
“His wingman saw the explosion,” said the Plano woman
who will honor the father she barely knew on Sunday at a special Father’s Day ceremony in Washington
Breaking NewsGet the latest breaking news from North Texas and beyond
GoogleFacebookBy signing up you agree to our Terms of Service and Privacy Policy
The event marks the 50th anniversary of American ground combat operations in Vietnam
It’s also the 25th anniversary of Sons and Daughters In Touch (SDIT)
a nationwide organization that unites children of service members who never returned from the Vietnam War
“He was proud of his country and proud of serving his country,” Von Lienen said of her father
Von Lienen will wash the section of the Vietnam Veterans Memorial Wall where her father’s is one of 58,000 names etched in the black granite surface
She performed the healing act five years ago when she attended her first SDIT ceremony
“I felt like I was able to do something for my dad for the first time,” recalled Von Lienen
Saavedra’s remains have never been recovered and he’s been listed as missing in action since his plane crashed on April 28
her family didn’t talk much about her father or the sacrifice he made for his country
“You didn’t want to talk about it because you didn’t know what they would say,” she said
a friend told her about the organization for children of fallen or missing service members
explaining her readiness to meet others who had lost fathers in Vietnam
But she also yearned to know more about the man she remembered through “a few snapshots in my head.”
After posting information about her dad on the online Vietnam Veterans Virtual Memorial Wall
she was pleased to see responses from his childhood friends and others who knew him
“I’d never heard any of his childhood stories,” said Von Lienen
She and her husband attended the 20th anniversary of the SDIT organization at The Wall in the nation’s capital
she got a chance to talk to others who had experienced the same kind of loss
“I started wanting to talk about him so he’s remembered and the sacrifice he’s made is remembered.”
the organization has become the support group she never had when she was growing up
And she’s pleased that events like the Father’s Day ceremony provide a way to give belated thanks to the unsung heroes of the war
“You just don’t want them to be forgotten,” Von Lienen said
Metrics details
Non-aureus staphylococci are commonly found on dairy farms
Two rarely investigated species are Staphylococcus (S.) cohnii and S
they may serve as an antimicrobial resistance (AMR) gene reservoir for harmful staphylococcal species
urealyticus isolates from German dairy farms were analyzed by whole-genome sequencing and AMR testing
The isolates harbored various AMR genes (aadD1
qacG/H) and exhibited non-wildtype phenotypes (resistances) against chloramphenicol
they showed reduced susceptibility to a number of beta-lactam antibiotics including cefoxitin (MIC 4–8 mg/L) and penicillin (MIC 0.25–0.5 mg/L)
The specificity of cefoxitin susceptibility testing for mecA or mecC gene prediction in S
A comparison with penicillin-binding protein (PBP) amino acid sequences of S
aureus showed identities of only 70–80% with regard to PBP1
urealyticus from selected German dairy farms show multiple resistances to antimicrobial substances and may carry unknown antimicrobial resistance determinants
The aim of our study was to evaluate the antimicrobial resistance potential of S
urealyticus isolates from German dairy farms
The isolates were analyzed by whole-genome sequencing (WGS) and AMR testing
AMR gene prediction was linked to the respective phenotypic resistance pattern
A special focus was set on beta-lactam antibiotic resistance and related AMR genes
since beta-lactams are commonly used to treat infections such as mastitis in livestock
Since NAS might serve as an AMR gene reservoir for S
which is a major animal and human pathogenic species
monitoring of multidrug-resistant NAS is of high importance
Phylogenetic analysis by TYGS of 15 presumptive S
cohnii isolates from German dairy farms reveals two main clusters of relationship
urealyticus DSM6718 indicates assignment of these isolates to the species
The isolates harbored various AMR genes with a variable distribution among the isolates
Resistance to the antibiotic classes aminoglycosides (aadD1
pleuromutilin–lincosamide–streptogramin (lnuA)
fosfomycin (fosB6) and to quaternary ammonium compounds (qacG/H) was predicted
The number of detected AMR genes per isolate varied between eight (1/15)
The fusF and dfrC genes were detected in all S. cohnii and S. urealyticus isolates (Table 1)
cohnii isolates) genes were only detected in some isolates or in only one of the two species
urealyticus) genes were each present in only one isolate
The mecA gene of isolate SC7 was located on a SCCmec type V
all mecA/mecC negative isolates were susceptible to oxacillin
cohnii isolate SC7 exhibited a non-wildtype phenotype to all beta-lactam antibiotics
Only the femA and gdpP genes were detected
Neighbor-joining phylogenetic tree with reference S
PBP2 and PBP3 amino acid sequences in comparison to PBPs of the investigated S
Amino acid sequence identity of PBPs from S
but apparently only leads to a reduced susceptibility to kanamycin
the 44% (4/9) or 33% (2/6) tetracycline resistance frequency of the S
urealyticus isolates in our study is rather low
Due to the limited data in public databases with respect to rpoB genes from S
urealyticus as well as to antimicrobial resistant S
it is difficult to link mutations in the rpoB gene of the isolates from our study to the manifestation of rifampicin resistance
urealyticus isolate SC11 harbored tetL without dfrK
data availability regarding AMR and its respective genes in S
urealyticus is extremely limited and for this reason it is unclear how reliable the annotation and function of the so far published S
Due to the lack of comparable public data from susceptible and non-susceptible strains
the association of AMR with specific gene alterations currently cannot be proven
the specificity of cefoxitin testing for the detection of mecA or mecC genes and respective methicillin resistance in S
but this needs to be confirmed on a larger strain collection
our findings provide further impetus for the investigation of antimicrobial resistance in NAS
since these organisms have been thus far largely overlooked in this respect
Ethical review and approval was not required for the study because sampling of quarter milk samples was carried out in accordance with German legislation within the framework of diagnostic investigations on the dairy farms
No ethical approval from the Institutional Ethics Committee or the National Animal Experimentation Council was required
Samples were collected by a trained veterinarian with consent from the owners of the animals
For evaluation of within farm genotypical differences
cohnii isolates were analyzed from two farms
DNA from one 1 µl inoculation loop filled with colonies was extracted using the Qiagen DNeasy Blood and Tissue Kit (Qiagen
Germany) according to the manufacturer´s protocol modified by a 10 µl lysostaphin addition to the lysis buffer
The DNA library was prepared using an Illumina Nextera DNA Flex kit (Illumina Inc.
USA) and the 150 bp paired-end sequencing run was performed on an Illumina NextSeq 500 instrument
cohnii complete circular genomes (NCBI accession numbers UHDA01000001.1
which were retrieved by NCBI Nucleotide search for “penicillin binding protein” and “Staphylococcus cohnii”
were extracted into an in-house database and S
urealyticus assembled sequences from our study were searched by NCBI blastn against these known PBP genes
Translation of DNA to amino acid sequences and alignment to PBP1-3 of S
aureus extracted from NCBI as well as among the different PBPs of the detected S
urealyticus isolates was conducted using MEGA X version 10.1.7 and ClustalW
A neighbor-joining phylogenetic tree was subsequently calculated comparing the PBP1-3 amino acid sequences from S
mgrA (AP017922.1) and mprF (HM140977.1) genes was checked by individual NCBI BLAST against S
aureus or Macrococcus caseolyticus (mecB and mecD) reference genes
aureus isolates ATCC 29213 and ATCC 25923 were used
The assembled sequences of all isolates in our study are deposited in NCBI under the BioProject PRJNA641762
Mahmmod, Y. S., Klaas, I. C., Svennesen, L., Pedersen, K. & Ingmer, H. Communications of Staphylococcus aureus and non-aureus Staphylococcus species from bovine intramammary infections and teat apex colonization. J. Dairy Sci. 101, 7322–7333. https://doi.org/10.3168/jds.2017-14311 (2018)
Condas, L. A. Z. et al. Distribution of non-aureus staphylococci species in udder quarters with low and high somatic cell count, and clinical mastitis. J. Dairy Sci. 100, 5613–5627. https://doi.org/10.3168/jds.2016-12479 (2017)
De Visscher, A., Piepers, S., Haesebrouck, F., Supre, K. & De Vliegher, S. Coagulase-negative Staphylococcus species in bulk milk: Prevalence, distribution, and associated subgroup- and species-specific risk factors. J. Dairy Sci. 100, 629–642. https://doi.org/10.3168/jds.2016-11476 (2017)
Wuytack, A., De Visscher, A., Piepers, S., Haesebrouck, F. & De Vliegher, S. Fecal non-aureus Staphylococci are a potential cause of bovine intramammary infection. Vet. Res. 51, 32. https://doi.org/10.1186/s13567-020-00761-5 (2020)
Mendoza-Olazaran, S. et al. Draft genome sequences of two opportunistic pathogenic strains of Staphylococcus cohnii isolated from human patients. Stand. Genomic Sci. 12, 49. https://doi.org/10.1186/s40793-017-0263-1 (2017)
Garza-Gonzalez, E. et al. Microbiological and molecular characterization of human clinical isolates of Staphylococcus cohnii, Staphylococcus hominis, and Staphylococcus sciuri. Scand. J. Infect. Dis. 43, 930–936. https://doi.org/10.3109/00365548.2011.598873 (2011)
Cave, R., Misra, R., Chen, J., Wang, S. & Mkrtchyan, H. V. Whole genome sequencing revealed new molecular characteristics in multidrug resistant staphylococci recovered from high frequency touched surfaces in London. Sci. Rep. 9, 9637. https://doi.org/10.1038/s41598-019-45886-6 (2019)
Loncaric, I. et al. Prevalence of methicillin-resistant Staphylococcus sp. (MRS) in different companion animals and determination of risk factors for colonization with MRS. Antibiotics (Basel) 8, 1. https://doi.org/10.3390/antibiotics8020036 (2019)
Nobrega, D. B. et al. Prevalence and genetic basis of antimicrobial resistance in non-aureus staphylococci isolated from Canadian dairy herds. Front. Microbiol. 9, 256. https://doi.org/10.3389/fmicb.2018.00256 (2018)
Seni, J. et al. Draft genome sequence of a multidrug-resistant caprine isolate of Staphylococcus cohnii subsp. urealyticus from Tanzania encoding ermB, tet(K), dfrG, fusF and fosD. J. Glob. Antimicrob. Resist. 18, 163–165. https://doi.org/10.1016/j.jgar.2019.07.006 (2019)
Cui, L. et al. Cfr-mediated linezolid-resistance among methicillin-resistant coagulase-negative staphylococci from infections of humans. PLoS ONE 8, e57096. https://doi.org/10.1371/journal.pone.0057096 (2013)
Chen, H. J. et al. A novel fusidic acid resistance determinant, fusF, in Staphylococcus cohnii. J. Antimicrob. Chemother. 70, 416–419. https://doi.org/10.1093/jac/dku408 (2015)
Fishovitz, J., Hermoso, J. A., Chang, M. & Mobashery, S. Penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus. IUBMB Life 66, 572–577. https://doi.org/10.1002/iub.1289 (2014)
Peacock, S. J. & Paterson, G. K. Mechanisms of methicillin resistance in Staphylococcus aureus. Annu. Rev. Biochem. 84, 577–601. https://doi.org/10.1146/annurev-biochem-060614-034516 (2015)
Lakhundi, S. & Zhang, K. Methicillin-resistant Staphylococcus aureus: Molecular characterization, evolution, and epidemiology. Clin. Microbiol. Rev. https://doi.org/10.1128/CMR.00020-18 (2018)
Shore, A. C. & Coleman, D. C. Staphylococcal cassette chromosome mec: Recent advances and new insights. Int. J. Med. Microbiol. 303, 350–359. https://doi.org/10.1016/j.ijmm.2013.02.002 (2013)
Chen, X. P. et al. Extreme diversity and multiple SCCmec elements in coagulase-negative Staphylococcus found in the Clinic and Community in Beijing, China. Ann. Clin. Microbiol. Antimicrob. 16, 57. https://doi.org/10.1186/s12941-017-0231-z (2017)
Zong, Z. & Lu, X. Characterization of a new SCCmec element in Staphylococcus cohnii. PLoS ONE 5, e14016. https://doi.org/10.1371/journal.pone.0014016 (2010)
Humphries, R. M. et al. Evaluation of surrogate tests for the presence of mecA-mediated methicillin resistance in Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus capitis and Staphylococcus warneri. J. Clin. Microbiol. https://doi.org/10.1128/jcm.02290-20 (2020)
Huse, H. K. et al. Evaluation of oxacillin and cefoxitin disk diffusion and MIC breakpoints established by the clinical and laboratory standards institute for detection of mecA-mediated oxacillin resistance in Staphylococcus schleiferi. J. Clin. Microbiol. https://doi.org/10.1128/jcm.01653-17 (2018)
Wu, M. T. et al. Evaluation of oxacillin and cefoxitin disk and MIC breakpoints for prediction of methicillin resistance in human and veterinary isolates of Staphylococcus intermedius group. J. Clin. Microbiol. 54, 535. https://doi.org/10.1128/JCM.02864-15 (2016)
Scholtzek, A. D. et al. Molecular characterization of equine Staphylococcus aureus isolates exhibiting reduced oxacillin susceptibility. Toxins (Basel) https://doi.org/10.3390/toxins11090535 (2019)
De Visscher, A. et al. Further evidence for the existence of environmental and host-associated species of coagulase-negative staphylococci in dairy cattle. Vet. Microbiol. 172, 466–474. https://doi.org/10.1016/j.vetmic.2014.06.011 (2014)
Bean, D. C., Wigmore, S. M. & Wareham, D. W. Draft genome sequence of Staphylococcuscohnii subsp. urealyticus isolated from a healthy dog. Genome Announc. https://doi.org/10.1128/genomeA.01628-16 (2017)
Schnitt, A., Lienen, T., Wichmann-Schauer, H., Cuny, C. & Tenhagen, B. A. The occurrence and distribution of livestock-associated methicillin-resistant Staphylococcus aureus ST398 on German dairy farms. J. Dairy Sci. https://doi.org/10.3168/jds.2020-18958 (2020)
Tenhagen, B. A. et al. Methicillin-resistant Staphylococcus aureus in cattle food chains—Prevalence, diversity, and antimicrobial resistance in Germany. J. Anim. Sci. 92, 2741–2751. https://doi.org/10.2527/jas2014-7665 (2014)
Tenhagen, B. A. et al. Short communication: Methicillin-resistant Staphylococcus aureus in conventional and organic dairy herds in Germany. J. Dairy Sci. 101, 3380–3386. https://doi.org/10.3168/jds.2017-12939 (2018)
Feßler, A. et al. Small antimicrobial resistance plasmids in livestock-associated methicillin-resistant Staphylococcus aureus CC398. Front. Microbiol. 9, 2063. https://doi.org/10.3389/fmicb.2018.02063 (2018)
Erythromycin resistance by ribosome modification
Mechanisms of resistance to macrolides and lincosamides: Nature of the resistance elements and their clinical implications
Ross, J. I., Eady, E. A., Cove, J. H. & Baumberg, S. Identification of a chromosomally encoded ABC-transport system with which the staphylococcal erythromycin exporter MsrA may interact. Gene 153, 93–98. https://doi.org/10.1016/0378-1119(94)00833-E (1995)
Feßler, A. T. et al. Characterization of methicillin-resistant Staphylococcus aureus CC398 obtained from humans and animals on dairy farms. Vet. Microbiol. 160, 77–84. https://doi.org/10.1016/j.vetmic.2012.05.005 (2012)
Argudín, M. A., Vanderhaeghen, W. & Butaye, P. Diversity of antimicrobial resistance and virulence genes in methicillin-resistant non-Staphylococcus aureus staphylococci from veal calves. Res. Vet. Sci. 99, 10–16. https://doi.org/10.1016/j.rvsc.2015.01.004 (2015)
Ida, T. et al. Identification of aminoglycoside-modifying enzymes by susceptibility testing: Epidemiology of methicillin-resistant Staphylococcus aureus in Japan. J. Clin. Microbiol. 39, 3115. https://doi.org/10.1128/JCM.39.9.3115-3121.2001 (2001)
Granados-Chinchilla, F. & Rodriguez, C. Tetracyclines in food and feedingstuffs: From regulation to analytical methods, bacterial resistance, and environmental and health implications. J. Anal. Methods Chem. 2017, 1315497. https://doi.org/10.1155/2017/1315497 (2017)
Kadlec, K., Entorf, M. & Peters, T. Occurrence and characteristics of livestock-associated methicillin-resistant Staphylococcus aureus in quarter milk samples from dairy cows in Germany. Front. Microbiol. 10, 1295. https://doi.org/10.3389/fmicb.2019.01295 (2019)
Frey, Y., Rodriguez, J. P., Thomann, A., Schwendener, S. & Perreten, V. Genetic characterization of antimicrobial resistance in coagulase-negative staphylococci from bovine mastitis milk. J. Dairy Sci. 96, 2247–2257. https://doi.org/10.3168/jds.2012-6091 (2013)
Campbell, E. A. et al. Structural mechanism for rifampicin inhibition of bacterial RNA polymerase. Cell 104, 901–912. https://doi.org/10.1016/S0092-8674(01)00286-0 (2001)
Kadlec, K., Feßler, A. T., Hauschild, T. & Schwarz, S. Novel and uncommon antimicrobial resistance genes in livestock-associated methicillin-resistant Staphylococcus aureus. Clin. Microbiol. Infect. 18, 745–755. https://doi.org/10.1111/j.1469-0691.2012.03842.x (2012)
Fu, Z. et al. Characterization of fosfomycin resistance gene, fosB, in methicillin-resistant Staphylococcus aureus isolates. PLoS ONE 11, e0154829. https://doi.org/10.1371/journal.pone.0154829 (2016)
Wassenaar, T. M., Ussery, D., Nielsen, L. N. & Ingmer, H. Review and phylogenetic analysis of qac genes that reduce susceptibility to quaternary ammonium compounds in Staphylococcus species. Eur. J. Microbiol. Immunol. (Bp) 5, 44–61. https://doi.org/10.1556/EUJMI-D-14-00038 (2015)
Bjorland, J. et al. Widespread distribution of disinfectant resistance genes among staphylococci of bovine and caprine origin in Norway. J. Clin. Microbiol. 43, 4363–4368. https://doi.org/10.1128/JCM.43.9.4363-4368.2005 (2005)
Couto, N., Belas, A., Kadlec, K., Schwarz, S. & Pomba, C. Clonal diversity, virulence patterns and antimicrobial and biocide susceptibility among human, animal and environmental MRSA in Portugal. J. Antimicrob. Chemother. 70, 2483–2487. https://doi.org/10.1093/jac/dkv141 (2015)
Methicillin resistance in staphylococci: Molecular and biochemical basis and clinical implications
Argudín, M. A. et al. Genetic diversity among Staphylococcus aureus isolates showing oxacillin and/or cefoxitin resistance not linked to the presence of mec genes. Antimicrob. Agents Chemother. 62, e00091-e118. https://doi.org/10.1128/AAC.00091-18 (2018)
Ba, X. et al. Novel mutations in penicillin-binding protein genes in clinical Staphylococcus aureus isolates that are methicillin resistant on susceptibility testing, but lack the mec gene. J. Antimicrob. Chemother. 69, 594–597. https://doi.org/10.1093/jac/dkt418 (2014)
Chen, S., Zhou, Y., Chen, Y. & Gu, J. fastp: An ultra-fast all-in-one FASTQ preprocessor. Bioinformatics 34, i884–i890. https://doi.org/10.1093/bioinformatics/bty560 (2018)
Ondov, B. D. et al. Mash: Fast genome and metagenome distance estimation using MinHash. Genome Biol. 17, 132. https://doi.org/10.1186/s13059-016-0997-x (2016)
Mikheenko, A., Prjibelski, A., Saveliev, V., Antipov, D. & Gurevich, A. Versatile genome assembly evaluation with QUAST-LG. Bioinformatics 34, i142–i150. https://doi.org/10.1093/bioinformatics/bty266 (2018)
Meier-Kolthoff, J. P. & Göker, M. TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy. Nat. Commun. 10, 2182. https://doi.org/10.1038/s41467-019-10210-3 (2019)
Feldgarden, M. et al. Validating the AMRFinder tool and resistance gene database by using antimicrobial resistance genotype–phenotype correlations in a collection of isolates. Antimicrob. Agents Chemother. https://doi.org/10.1128/AAC.00483-19 (2019)
European Food Safety Authority. Technical specifications on the harmonised monitoring and reporting of antimicrobial resistance in methicillin-resistant Staphylococcus aureus in food-producing animals and food. EFSA J. 10, 1. https://doi.org/10.2903/j.efsa.2012.2897 (2012)
Download references
Karin Eulenberger and Natalie Morgenstern for supporting the identification of study farms
Pascal Witt and Ylanna Kelner-Burgos for NGS preparation and Heidi Wichmann-Schauer for help with study conception
We also thank Carlus Deneke and Simon Tausch for bioinformatics analyses
we thank the German Federal Ministry of Education and Research (BMBF) for funding our research in the framework of the project #1HealthPREVENT (Grant No
Open Access funding enabled and organized by Projekt DEAL
German Federal Institute for Risk Assessment (BfR)
Sven Maurischat & Bernd-Alois Tenhagen
T.L.: conceptualization and design of the study
A.S.: conceptualization and design of the study; samples collection; laboratory work; critically reviewing the manuscript
J.A.H.: laboratory work; critically reviewing the manuscript
critically reviewing the manuscript; S.M.: data analysis
critically reviewing the manuscript; B.-A.T.: conceptualization and design of the study
All authors read and approved the final manuscript
The authors declare no competing interests
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations
Download citation
DOI: https://doi.org/10.1038/s41598-021-85461-6
Anyone you share the following link with will be able to read this content:
a shareable link is not currently available for this article
Sign up for the Nature Briefing newsletter — what matters in science
As the German foal selection show circuit is in full swing
six events were scheduled for the weekend of 23 - 26 June 2011
Floriscount and Licotus sired the champion colts in Westerwald/Taunus
Instertanz and Quaterback were the sires of the champion fillies
On 23 June 2011 the foal selection show organized by breeders' group Lahn-Dill in Westerwald-Taunus near Niederzeuzheim
produced four foals that were selected for the German Foal Championships in Lienen
In the colts' category there were two winners
Gisela Strecker's Sandro Hit x Frisbee colt tied for first place with Bianca and Jorg Opper's Ampere x Fidertanz colt
The winning filly was a Stoiber x Damon Hill offspring
Two days later on 25 June a big selection show took place in Isterberg
organized by the breeders' group Grafschaft Bentheim - Emsland
They split up the colts' category in dressage and show jumping
Nicole Wanning's amazing dressage bred colt Flatrate (by Floriscount x Metternich) took highest honours receiving 9s and 10s from the judges for gaits and conformation
Hermann Bengfort's Apache x Furst Piccolo colt ranked second
In the filly category Gestut Letter Berg's Instertanz x Laureus mare bested the field and left a Feuerspiek x Latimer filly behind her
the foal selection show held at Gestut Bon Homme in Werder
proved that the "older" stallion are still in fashion and scoring well
Two colts were selected for Lienen and the winner was Paul Paries' Licotus x Quando Quando
Maik Katzke's Damon Hil x Quando Quando was second
The filly division was dominated by Quaterback offspring taking first and second position
The winning filly was one out of a Donnerhall dam (bred by Silke Pelzer)
The runner up is out of a Lauries Crusador xx broodmare
Photo © Johannes Krage - courtesy Wanning
Foal Selection Show - Westerwald/Taunus 2011
The 5-year old Global Player and the 6-year old Feingefühl topped the board in the Bundeschampionate qualification classes at the regional show in Lienen on Thursday 6 May 2021
A field of 21 competitors gathered in rainy weather in Lienen to earn a ticket to Warendorf and no less than 8 pairs achieved the required minimum standard of 8.0 or higher
Eva Möller and Helgstrand & Schockemöhle's Oldenburg stallion Global Player (by Grand Galaxy Win T x Don Schufro) aced the division with a whopping 9.0 total score
Esther Lobeck and Katja Westendarp rewarded the Oldenburg licensing champion stallion with 8.5 for walk
and 9 for submission and general impression
The runner-up was Anja Wilimzig aboard the Warendorf State Stud's Westfalian stallion Felissimo (by Franziskus x Belissimo M)
submission and general impression to finish second with 8.5
Möller also steered Schockemöhle & Helgstrand's Hanoverian stallion Danciero (by Dancier x Floriscount) to a third place with 8.3
7.5 for submission and 8.5 for general impression
Fourteen pairs contested the 6-year old qualifier which was judged by Katja Westendarp
Esther Lobeck and Heinrich Plaas-Beisemann
German professional Sandra Kötter and Gaby Heye-Hammerlage's Hanoverian mare Feingefühl (by Fürstenball x Farewell III) aced the class with 8.2 points
8.5 for submission and 8 for general impression
Two pairs tied in second place with 8.1 points
Former German youth rider Kristin Biermann steered Gestut Sprehe's Oldenburg stallin Delano (by Diamond Hit x Fielmann) to that second place score
7.5 for submission and 8 for general impression
Polish Beata Stremler also placed second with the Hanoverian gelding For Magic (by For Romance x A Jungle Prince)
7.5 for canter and submission and 8 for general impression
A Furstenball colt and a Surprice filly were proclaimed champions at the 2011 German Foal Championships in Lienen
Twenty-two foals in total made it into the end ring where the judges picked the national champion and ranked the other foals
Twelve colts made it to the final championship ring on Saturday 16 July. Bernd Ilting-Reuke's Furstenball I x Sir Donnerhall colt scored a staggering total of 98 points and was a league of his own in the colt category
The bay referred Bianca and Jörg Opper's Ampere x Fidertanz colt to a second place with 92,50 points
Hermann Peter Rabeler's Furst Nymphenburg x Donnerhall was third with 92.000 points
On Sunday 17 July 2011 ten fillies were picked to battle it out for the national championship title
Petra Fockenberg's in-bred filly by Surprice (by Sir Donnerhall I x Prince Thatch xx) offspring out of a Samarant (Sandro Hit x Brentano II) dam scored 93,5 points to best the filly division at this year's national championships
She was followed very closely by the runner up
Norbert Schmidt's Quaterback x Lauries Crusador xx finished third with 92,50
Photo © Kiki Beelitz
Pauli and Stoke City Football Club kicks off with a friendly match on Tuesday evening
A few hours before kick-off officials of both sides - Tony Scholes and Mark Cartwright for Stoke and Andreas Rettig and Ewald Lienen for FCSP – explained the reasoning behind the cooperation
which came about very quickly and professionally," said FCSP commercial director Andreas Rettig
"The motivation for us is to keep improving all the time
and in Stoke we've found a partner who can help us do that."
Though sport will form the focus of the partnership
there are opportunities in other areas as well
"We're also hoping to learn from each other in the area of marketing," said Rettig
"Some of our partners are looking to expand their presence in the UK market and vice-versa
The UK is one of our most important markets as far as merchandise is concerned and we also hope to benefit from Stoke's expertise in the areas of social media and club TV."
Rettig continued: "It makes sense to look for a partner in England because the Premier League is the biggest league in the world in commercial terms
In Stoke City we've found a club with a long tradition for whom the fans are extremely important
Stoke haven't put their ticket prices up in ten years and have also funded free away travel for the fans for quite some time now."
Stoke CEO Tony Scholes is also very much looking forward to the partnership
"We quickly realised that the two clubs have a lot in common," he said
Pauli and are convinced we can learn a lot from each other – both on and off the pitch."
Scholes and the rest of the Stoke City delegation arrived in Hamburg yesterday lunchtime and got to know St
Pauli on an extensive three-hour tour of the district
In addition to many similarities he has noticed quite a few differences: "There'll be some things we can't put into practice at home but there are others we'll certainly benefit from," said Scholes
Pauli as a "unique club" before issuing an invitation to FCSP fans to visit Stoke to get to know the club
Stoke City technical director Mark Cartwright will cooperate very closely with his FCSP counterpart Ewald Lienen in future
Cartwright visited the Millerntor for the first time for the goalless draw against SV Sandhausen in early April
The game itself didn't bowl him over but the hospitality certainly did
"The welcome from Andreas and his team was exceptional," he said before adding: "It soon became clear that there's a lot we can do together
From the sport perspective we think the partnership offers great opportunities for working together
The style and intensity of our play are similar
We also see big chances in the area of player development."
Ewald Lienen also believes the partnership offers great opportunities
"Sport will be at the heart of the cooperation," the 63-year-old said
"We're obviously interested in building a really good squad full of talented young players
while Stoke are looking to loan out young players to allow them to gain match practice in a high-quality league."
"The two clubs also have an opportunity to coordinate their squad planning," Lienen continued
"It's not merely a question of taking players on loan
Stoke can help us sign players who in two or three years' time may have progressed enough to play for them
For us it's an opportunity to get players we couldn't otherwise afford
Stoke have the chance to let players play here who at some point will have the quality needed to play for them."
Lienen has also identified opportunities for the clubs to improve their scouting operations and learn from each other's academy work
"Youth coaches could spend time learning from their counterparts at the other club and our youth teams could fly over for two or three weeks during the holidays to gain valuable experience" he said
"There are so many possibilities for us to talk about
but that's all in the future," he concluded
The two technical directors Ewald Lienen (left) and Mark Cartwright (right) will work together very closely in future
"HORSE24 Champions" - what could be more apt and appropriate for this auction premiere following the HORSE24 German Foal Championships last weekend in Lienen than this name
All nine hand-picked foals put up for the online auction from 20 to 23 July were awarded gold
silver or bronze or showed up in the finals
Among them are the amazing winner in the riding pony division and the winner of the Goldfever-class
thanks to his particularly supple movements
even made it to the final of the best dressage foals
Invincible (1) is the celebrated winner in the dressage colt division
is half-brother to Patrik Kittel's up-coming star Sir Floresco
Fiero (by Fynch Hatton x San Amour)Vice-Champion Fiero (4)
a son by the Champion Stallion and Federal Champion Fynch Hatton OLD
also displays qualities of a true future stallion personality
The silver medallist in the filly division descends from the line of Olympic team bronze medallist Rainier with his American rider Robert Dover and Isabell Werth's Grand Prix Champion Aleppo-S: Flawless (5)
Her sire For Gold carries the valuable performance blood of Olympic Champion and three-time European Champion Weihegold OLD
The winning trio is completed by bronze medallist Belle (6)
who even earned three times the maximum score of 10 for her outstanding movements
The gorgeous daughter by Bond is out of the same line than World Cup show jumping winner Arielle under the British rider William Whitaker
Flawless (by For Gold x Florencio)For all important details on the nine "HORSE24 Champions"- foals, click on the auction page of www.horse24.com where free registration will also be possible
Placing bids will be possible as of Thursday
Please contact HORSE24 founder Simon Kohlenbrenner should you require additional information and personal advice
Public Law and Judicial Review
Associate Member Dr Christina Lienen will speak on 19th April 2024 at the Third Critical Perspectives on Human Rights Conference
organised by The City College of New York’s Division of Interdisciplinary Studies from 17-19 April 2024
She will be part of the panel “History
and Human Rights” alongside academic colleagues from the UK and the United States
Her talk – “Human Rights as a Vehicle to Project Rather than to Protect” – adds to the growing discourse offering a critical examination of both the legacy and the present state of international human rights
she argues thar human rights have to a large extent been used as a vehicle to project a certain image on the purported benefactors of human rights
and to keep said benefactors in positions of control
rather than to achieve the equitable protection of universal values
Website by Square Eye Ltd.
The ‘cult’ football club's behaviour has made headlines again
I would like to be emailed about offers, events and updates from The Independent. Read our Privacy notice
St Pauli, the famous ‘cult’ football club based in Hamburg, have made headlines once again after announcing a new signing in a peculiar fashion.
The 2. Bundesliga side, known for their brown kit, unofficial skull and crossbones emblem and adoption of left-leaning politics, presented Marvin Ducksch to media on Wednesday morning.
However, Ewald Lienan, the club’s manager, could not make the unveiling – so, in his place, a unidentified man wore a mask of the 62-year-old coach.
Ducksch, a 22-year-old striker signed from Borussia Dortmund, looks rather nonplussed by the stunt in the photograph, despite saying that he is “looking forward to the challenge at St Pauli.”
After the image prompted questions from fans on social media regarding the whereabouts of Lienen, St Pauli confirmed that a mystery man in a mask had stood in for their manager and described it as “a one-off”.
The club later issued a reaction from Lienen to the signing; he said: “With Marvin we get an attacker who has good technique and a very good end product.”
Join thought-provoking conversations, follow other Independent readers and see their replies
A German firefighter was killed and another was seriously injured in a gas explosion Saturday
The blast occurred at about 11:30 a.m. in an apartment building in Lienen in the country's Steinfurt district, German news outlet Bild reports
and they alerted firefighters as they left the building
Crews were trying to determine the source of the gas smell when the explosion happened
Firefighters also were struck by debris in the blast
was killed after being buried beneath the rubble
Another firefighter was pulled from the wreckage and suffered serious injuries
Dr Christina Lienen, Associate Member of Chambers, has published a new book, Shaped by the Nuanced Constitution: A Critique of Common Law Constitutional Rights
which is published by leading publisher Hart Publishing (Bloomsbury)
analyses significant recent public law judgments
R (Privacy International) v Investigatory Powers and R (Begum) v Special Immigration Appeals Commission
It argues that neither common law constitutionalism nor political constitutionalism adequately captures the nature of public law litigation because neither is fully able to account for the co-existence and interplay between parliamentary sovereignty and the rule of law
Advancing the idea of a ‘nuanced’ constitution instead
the book provides an in-depth analysis of common law constitutional rights
The book is available as an e-book and in hardback via the publisher’s website.
56th annual event rolls into Calgary's Stampede Park April 5-7 showcasing classics, collectibles and celebrities
Join the conversation You can save this article by registering for free here. Or sign-in if you have an account
“Other than some of the bodywork and paint
everything on this Camaro was done in my garage,” Lienen says
It’s also something of a social activity and offers a way to spend time together.”
presents an opportunity for enthusiasts to stay current on custom vehicle trends
except for the COVID years of 2021 and ’22
And he’s no stranger to showing a vehicle at the World of Wheels
he displayed a custom 1972 Nova but did not enter the vehicle in competition
Lienen has entered his 1967 Camaro SS in in the 26th annual Jim Leslie Memorial Award Competition
Remove Vehicle NEXTArticle content“I’ve been into cars my whole life,” Lienen says
“My dad was a car guy who was into hot rods and cool cars and he built a 1970 Chevy Nova SS
He’d tinker in the garage and both my older brother and I would help him.” Originally from Montreal
Lienen’s family moved to Edmonton when he was 12 years old
In that Alberta city Lienen built his first custom car
one I’d take to work,” Lienen says of his El Camino
built with a 421 cubic-inch engine and 4-speed transmission on nitrous that would run in the 9s out at Castrol Raceway.”
but in 2014 Lienen saw a Craigslist ad offering the 1967 Camaro for sale in Surrey
but it was an original Canadian Camaro that had sold new out of Dueck Chevrolet in Vancouver
Lienen and his friend Jerry Butler drove out with a trailer and bought the project
because the guy I purchased it from had been planning to restore the Camaro and had been acquiring new old stock parts for it for years,” he says
took on the job of cutting out and replacing rusted quarter panels and floors
Lienen began restoring and detailing many of the Camaro’s components
“I had that motor from back in the day,” Lienen says
“I ran it in a 1968 Beaumont but had pulled it and set it aside
Get your instant cash offer in under 2 minutes
Full Screen is not supported on this browser version
You may use a different browser or device to view this in full screen
the motor was connected to a 4-speed Muncie transmission
“Things started to get out of control.” Lienen continues
with leaf spring rear suspension and rally wheels
But then I spent a little more money and changed the suspension to a 4-link system and bought aftermarket wheels
all of the hardware is ARP (Automotive Racing Products) stainless steel and the transmission was upgraded to a Tremec T56 Magnum 6-speed.”
Greg Williams is a member of the Automobile Journalists Association of Canada (AJAC)
Contact him at 403-287-1067 or gregwilliams@shaw.ca
you will need these must have tools and accessories
Looking to make car ownership more comfortable
Here are our top under-$100 accessories for your car
Here are the best windshield wipers on the market right now—these windshield wipers are highly rated both by users and testers
You don't need to buy a new car to add Apple CarPlay or Android Auto
There's lots of aftermarket gear out there
but get these add-ons first—they'll make your daily drive more comfortable and safe
transmission or republication strictly prohibited
This website uses cookies to personalize your content (including ads), and allows us to analyze our traffic. Read more about cookies here. By continuing to use our site, you agree to our Terms of Use and Privacy Policy
You can only pick 5 vehicles to compareEdit your picks to remove vehicles if you want to add different ones
You can only add up to 5 vehicles to your picks
Cornerstone Climate
full-time academic and Associate Member of Cornerstone
lists her key takeaways from Day 6 of COP28
I attended the 2023 Climate Law & Governance Day
co-hosted by different educational institutions and other organisations
the University of Cambridge and the Centre for International Sustainable Development Law
Cornerstone Climate were proud to be among the sponsors for today’s event
which featured a wide range of topics addressing climate law
Here are a seven highlights and insights from the different panel sessions of today’s event
which I hope will be of interest to environmental lawyers
lawyers with different specialisations and non-lawyers alike:
A Franziskus colt and a Quitender filly were proclaimed 2012 German Foal Champions at the National Foal Show on 13 - 15 July 2012
For the 13th time the German Foal Championships took place in the Westfalian town of Lienen
A collection of high quality foals was invited to compete in Lienen after a string of selection trials all over Germany during May and June
For the first time in history a show jumping bred foal won the show jumping and overall foal title at the Nationals
Anja van Horn's Quintender x Compliment filly became the champion
Frank Grote's filly by Sir Donnerhall x Compliment was named reserve champion
while Breeders' Group Werth bred the third placed filly by Quaterback x Sandro Hit
Photos © LL-foto.de
Related LinksFurstenball and Surprice Sire Champions at 2011 German Foal Championship in Lienen
Browse over 5,400 law jobs. Get jobs by email
Our Privacy Policy has changed. Please click here to read about how we process your data in compliance with the General Data Protection Regulation (GDPR)
By James E Hurford2025-02-23T00:34:00+00:00
The UK’s constitution has much in common with a bacterial flagellum
Flagella are sophisticated little organs that rotate like outboard motors and allow organisms to move around
They are assembled from dozens of separate elements that originally had different functions and which have been co-opted to form part of the new system
which represents numerous parts co-opted for new purposes
an extension of judicial distaste for ouster clauses there
and something new and apparently coherent emerges
One consequence is a debate about the essential character of the constitution
Some commentators and judges argue it is a political constitution
whereby the sole restraint on parliament’s legislative power is the political consequences of its actions (the classic Diceyan position)
Others argue it is a common law constitution
employing fundamental common law principles
can place substantive limits on parliament
Lienen argues for a third way – which she calls ‘nuanced’ – which is created by judges ‘engaging with substantive arguments about values and interests which are given legal status at the same time as according a fundamental status
she claims the courts are increasingly acknowledging various common law constitutional rights
require more intense scrutiny of any legislation that may adversely affect those rights
even – theoretically – to the point of disapplying the offending legislation
Her argument is fascinating and occasionally compelling
I am unconvinced that ‘nuanced’ represents a new understanding of the constitution
courts have been willing to read legislation more restrictively where some key right is engaged
No court has shown willingness to challenge sovereignty
notwithstanding the dicta of lords Steyn and Hope
and Baroness Hale in Jackson v Attorney General two decades ago
it is hard to see the constitution as anything other than political
James E Hurford is a solicitor at the Government Legal Department
Paul Bennett reviews Snell’s Equity (35th edition) by Steven Elliott KC
Julian Phillips reviews Tom Gaisford's 'Sanctuary'
James E Hurford reviews 'Law at the Frontiers of Biomedicine: Creating
Browse over 3,200 law jobs. Get jobs by email
Michael Freeman reviews 'Handbook on Crime and Inequality'
Patrick Keane reviews 'Beauty and the Law' by Mark Fowler
Judicial Individuality on the UK Supreme Court Lewis Graham £85
Bloomsbury Supreme court justice gown Socrates is supposed to have said that a judge’s duties are ‘to hear courteously; to answer wisely; to consider soberly; and to decide impartially’
Charity Explorer provides a reputable reference tool for solicitors
will-writers and their clients who want to leave a legacy or charitable gift
Whether you are looking for legal expert witnesses
barristers chambers or any other general legal service
the Legal Services Directory will provide a suitable option
Site powered by Webvision Cloud