A farmer (73) fell from a crane while making hay and died The accident occurred on Tuesday evening in Waldkirch SG a fatal accident occurred on a farm at Egelsee A 73-year-old man was using a crane to lift hay from the ground onto a hayloft he fell several meters to the ground from the hay crane cabin The casualty was a 73-year-old Swiss national In addition to several patrols and specialists from the St.Gallen cantonal police the rescue service with specialist medical personnel and the fire department were also deployed Sensirion does not see itself affected by US tariff policy New regulation is driving the sensor specialist’s business this year Mr. von Waldkirch, gas leakage sensors are expected to drive Sensirion’s growth. Are the plans on track in view of the turmoil in the USA? The basis for our confidence in these sensors lies in a US regulation that came into force at the beginning of the year: all new air conditioning systems in the US must be equipped with a more climate-friendly refrigerant – larger systems must also be fitted with a leakage sensor. The direct impact on our business is limited. We mainly supply our products to Mexico. The greater risk lies in the introduction of tariffs on Mexican products. This would make our customers’ air conditioning systems more expensive to import into the USA and thus affect demand. That depends heavily on future political developments – and is difficult to predict. The challenge lies precisely in this uncertainty. For us – in fact for the entire global economy – a lack of predictability is poison. This uncertainty has a direct impact on investment decisions. Could you expand Sensirion’s production in the USA? There are no plans to set up our own US production facility. Firstly, something like that takes time, and secondly, skilled workers are in short supply in the USA. The high local wages would compensate for the tariffs saved. It would be conceivable to work with a local partner for certain products that we do not manufacture ourselves. Sensirion grew by 19% last year. Mainly thanks to leakage sensors? Two thirds of sales growth in 2024 came from new business, with leakage sensors playing a particularly important role in the second half of the year. New business with a German car manufacturer also brought additional growth. Here, our particulate matter sensors measure the air quality in cars and control the ventilation system accordingly. Analysts expect leakage sensors to contribute around CHF 50 million to sales in 2025 alone. Is that realistic? We expect this new product category to make a strong contribution to growth this year. How high the contribution to sales will ultimately be depends on many factors; it will be somewhere between CHF 10 and 100 million. The contribution will depend in particular on further market development and the market share that can ultimately be achieved. Typically, our customers provide a forecast on the basis of which we plan. We are also in close contact with our most important customers. So far, everything is going as expected. «Some of our customers have become a little more cautious due to the expected tariffs.» Could there be similar requirements to those in the USA in other countries that Sensirion could benefit from? We do not expect this for the A2L refrigerant. But a climate-friendly refrigerant is currently being discussed in Europe, which could possibly trigger a demand for leakage sensors in the future. However, decisions on this are still pending. We have made a good start to the year. Some of our customers have become a little more cautious due to the expected tariffs. Overall, business is going well, but the uncertainty is noticeable. Probably mainly in the automotive sector, which will be heavily affected by the US tariffs. Some customers from the automotive market already reduced their sales forecasts last year. Yes, we are again expecting growth of 12 to 27% to CHF 310 to 350 million in 2025. In addition, our goal is to return to our normal profitability level this year, i.e. to achieve a margin in the mid to high teens at EBITDA level. Sensirion CEO Marc von Waldkirch confirms the guidance for 2025.How do you intend to achieve this? Primarily through growth: as a growth company, we invest 18 to 20% of sales in research and development. We also have high fixed costs for our cleanrooms. The best way for us to increase profitability is therefore to increase sales and thus our capacity utilization. This year, leakage sensors will be the growth driver. We also expect a recovery in the medical sector. The full warehouses after the pandemic have been cleared. We don’t talk about that in the early stages because our competitors are just as interested. «Yes, our product portfolio may expand in the future, that much I can say for sure.» But are we talking about the area of flow and environmental sensors – or something completely different? Yes, our product portfolio may expand in the future, that much I can say for sure. Acquisitions can play a supporting role: On the one hand, we acquire technologies; on the other, we use acquisitions to position ourselves in a market. There is nothing to report at the moment. How important is Switzerland as a location? Switzerland is and will remain our central location. Innovation is essential for us, and for this we not only need talent, but also a corporate culture that promotes creativity and collaboration across departments. Switzerland is also the location of our chip production. This proximity between research, development and production gives us a significant advantage. Are the plans to expand production in Stäfa still valid despite the uncertain global situation? Yes, we have to look at a time horizon of ten, fifteen or even twenty years when making such decisions – not a legislative period. With a long-term perspective, would you recommend a good friend to buy Sensirion shares? Of course I am. Since I joined the company almost twenty years ago, I have received every bonus in shares and have never sold shares since. Sensirion was a winner during the pandemic, but business slowed down afterwards due to full warehouses. Now the company could herald the next growth cycle thanks to environmentally friendly regulations in the US. The contribution of sensors that detect gas leaks in air conditioning systems will be «somewhere between CHF 10 and 100 million in 2025», explains Sensirion CEO Marc von Waldkirch in an interview with «Finanz und Wirtschaft». The specialist for flow and environmental sensors was founded in 1998 as a spin-off from ETH Zurich. Sensirion's sensors are world-class in many areas. For example, the company sees itself as the market leader for relative humidity and temperature sensors in the automotive industry and one of the leading manufacturers of flow measurements in medical technology, such as those required for ventilators. It is also active in the industrial and consumer goods markets. Sensirion's management responded to the crisis following the exceptional economic situation during the pandemic by cutting costs. As a result, costs fell by around CHF 9 million on an annualized basis last year. This was accompanied by strong organic sales growth of 22.1% to sales of CHF 276.5 million in 2024. The adjusted EBITDA margin amounted to 10.5%, rising to 15.5% in the second half of the year, which is already back within the company's medium-term target range. Hier wird Inhalt angezeigt, der zusätzliche Cookies setzt. An dieser Stelle finden Sie einen ergänzenden externen Inhalt. Falls Sie damit einverstanden sind, dass Cookies von externen Anbietern gesetzt und dadurch personenbezogene Daten an externe Anbieter übermittelt werden, können Sie alle Cookies zulassen und externe Inhalte direkt anzeigen. Volume 12 - 2018 | https://doi.org/10.3389/fncel.2018.00473 This article is part of the Research TopicSynaptic Loss and NeurodegenerationView all 14 articles Synapse loss has detrimental effects on cellular communication leading to network disruptions within the central nervous system (CNS) such as in Alzheimer’s disease (AD) AD is characterized by a progressive decline of memory function The two main neuropathological hallmarks are amyloid-β (Aβ) plaques and neurofibrillary tangles In the brain of AD patients and in mouse models of AD several morphological and functional changes such as microgliosis and astrogliosis around Aβ plaques as well as dendritic and synaptic alterations we will summarize the current literature on synapse loss in mouse models of AD and discuss current and prospective treatments for AD Although the cause of synapse loss has not yet been fully elucidated these results support the idea that soluble forms of Aβ are toxic to synapses we will focus in this review more on the role of microglia and astrocytes and synapse loss implicating microglia in sculpting synaptic connectivity Microglia might then be recruited to the Aβ tagged synapse and induce the removal of this complex Microglia and astrocytes cluster around amyloid-β (Aβ) plaques (white arrows) in the brains of amyloid precursor protein (APP) transgenic mice red) can be found closely associated with Aβ plaques (6E10 with dystrophic neurites appearing in the vicinity of Aβ plaques (GFP) red) can be found in close proximity to Aβ plaques (6E10 Further investigations of neuron-glia signaling pathways and their disruption in neurodegenerative diseases are necessary for the development of new successful therapies that are promising due to the early involvement of glia in the disease process Although our knowledge regarding the mechanism underlying AD pathogenesis has improved over the last decades, there is still no cure available. Moreover, open questions concerning memory and synapse loss, as well as gliosis and related neuronal damage, still remain (De Strooper and Karran, 2016) Further investigations on the function of tau in mouse models of AD will provide insights regarding the role of tau in AD Future research on the microglia function and dysfunction in CNS disorders regulating plasticity and neurogenesis will undoubtedly play a predominant role in the search for an effective cure Besides the physical degeneration of synapses in AD and other neurodegenerative diseases it is unclear which role glial cells play during the process of synapse loss Further research will hopefully provide more insights into the role of glial cells and their contribution to synapse loss in particular at earlier pre-depositing stages when synapses are already vulnerable Future preclinical treatment approaches should combine pharmacological non-pharmacological and behavioral studies SZ-W and MM-L contributed equally to this work This work was supported by the Deutsche Forschungsgemeinschaft (DFG) ME 3542/2-1 (MM-L) The article processing charge was funded by the German Research Foundation (DFG) and the University of Freiburg in the funding programme Open Access Publishing The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest Dark microglia: a new phenotype predominantly associated with pathological states γ-secretase inhibition reduces spine density in vivo via an amyloid precursor protein-dependent pathway Multiple events lead to dendritic spine loss in triple transgenic Alzheimer’s disease mice Dynamics of the microglial/amyloid interaction indicate a role in plaque maintenance Impairment of in vivo calcium signaling in amyloid plaque-associated microglia Quantitative decrease in synaptophysin message expression and increase in cathepsin D message expression in Alzheimer disease neurons containing neurofibrillary tangles Emerging roles of astrocytes in neural circuit development Natural oligomers of the amyloid-β protein specifically disrupt cognitive function Enhanced cognitive activity—over and above social or physical activity—is required to protect Alzheimer’s mice against cognitive impairment Homeostatic responses by surviving cortical pyramidal cells in neurodegenerative tauopathy D’Amore In vivo multiphoton imaging of a transgenic mouse model of Alzheimer disease reveals marked thioflavine-S-associated alterations in neurite trajectories Colony-stimulating factor 1 receptor inhibition prevents microglial plaque association and improves cognition in 3xTg-AD mice Excitability and synaptic communication within the oligodendrocyte lineage The cellular phase of Alzheimer’s disease Amyloid-β peptide oligomers disrupt axonal transport through an NMDA receptor-dependent mechanism that is mediated by glycogen synthase kinase 3β in primary cultured hippocampal neurons Disease-associated microglia: a universal immune sensor of neurodegeneration Metabotropic P2Y1 receptor signalling mediates astrocytic hyperactivity in vivo in an Alzheimer’s disease mouse model NG2-glia and their functions in the central nervous system Immunotherapy alleviates amyloid-associated synaptic pathology in an Alzheimer’s disease mouse model Pharmacological inhibition of BACE1 impairs synaptic plasticity and cognitive functions Analysis of the putative role of CR1 in Alzheimer’s disease: genetic association Absence of C1q leads to less neuropathology in transgenic mouse models of Alzheimer’s disease Genetic modulation of soluble Aβ rescues cognitive and synaptic impairment in a mouse model of Alzheimer’s disease Aβ oligomers inhibit synapse remodelling necessary for memory consolidation Dendritic pathology in prion disease starts at the synaptic spine β-secretase protein and activity are increased in the neocortex in Alzheimer disease Plaque-derived oxidative stress mediates distorted neurite trajectories in the Alzheimer mouse model Expression profile of transcripts in Alzheimer’s disease tangle-bearing CA1 neurons doi: 10.1002/1531-8249(200007)48:1<77::aid-ana12>3.3.co;2-1 Alzheimer’s disease risk gene CD33 inhibits microglial uptake of amyloid β neurodegeneration and protein aggregation in the retina as ocular biomarkers for Alzheimer’s disease in the 3xTg-AD mouse model Abnormal phosphorylation of the microtubule-associated protein tau (tau) in Alzheimer cytoskeletal pathology Long-term two-photon transcranial imaging of synaptic structures in the living brain Long-term dendritic spine stability in the adult cortex TREM2 variants in Alzheimer’s disease Take five—BACE and the γ-secretase quartet conduct Alzheimer’s amyloid β-peptide generation Genome-wide association study identifies variants at CLU and PICALM associated with Alzheimer’s disease Models of amyloid seeding in Alzheimer’s disease and scrapie: mechanistic truths and physiological consequences of the time-dependent solubility of amyloid proteins Many neuronal and behavioral impairments in transgenic mouse models of Alzheimer’s disease are independent of caspase cleavage of the amyloid precursor protein Long-term potentiation depends on release of D-serine from astrocytes Environmental enrichment enhances cellular plasticity in transgenic mice with Alzheimer-like pathology Genome-wide association study of Alzheimer’s disease with psychotic symptoms Complement and microglia mediate early synapse loss in Alzheimer mouse models Plaque-independent disruption of neural circuits in Alzheimer’s disease mouse models BACE1 deletion in the adult mouse reverses preformed amyloid deposition and improves cognitive functions Amyloid-dependent and amyloid-independent stages of Alzheimer disease Dendritic function of tau mediates amyloid-β toxicity in Alzheimer’s disease mouse models GABA from reactive astrocytes impairs memory in mouse models of Alzheimer’s disease Variant of TREM2 associated with the risk of Alzheimer’s disease and PICALM as alzheimer disease risk loci and reveals interactions with APOE genotypes A unique microglia type associated with restricting development of Alzheimer’s disease Alzheimer’s disease: synapses gone cold Oligomeric amyloid β associates with postsynaptic densities and correlates with excitatory synapse loss near senile plaques The TREM2-APOE pathway drives the transcriptional phenotype of dysfunctional microglia in neurodegenerative diseases Synchronous hyperactivity and intercellular calcium waves in astrocytes in Alzheimer mice Phosphorylation of amyloid β (Aβ) peptides—a trigger for formation of toxic aggregates in Alzheimer’s disease Genome-wide association study identifies variants at CLU and CR1 associated with Alzheimer’s disease Environmental enrichment reduces Aβ levels and amyloid deposition in transgenic mice Channel-mediated tonic GABA release from glia A specific amyloid-β protein assembly in the brain impairs memory Soluble oligomers of amyloid β protein facilitate hippocampal long-term depression by disrupting neuronal glutamate uptake Soluble Aβ oligomers inhibit long-term potentiation through a mechanism involving excessive activation of extrasynaptic NR2B-containing NMDA receptors Neurotoxic reactive astrocytes are induced by activated microglia A peephole into the brain: neuropathological features of Alzheimer’s disease revealed by in vivo two-photon imaging Chronic γ-secretase inhibition reduces amyloid plaque-associated instability of pre- and postsynaptic structures Synaptic signaling between neurons and glia lowers Aβ levels in the cerebrospinal fluid and plasma in nonhuman primates CrossRef Full Text | Google Scholar Maletic-Savatic Rapid dendritic morphogenesis in CA1 hippocampal dendrites induced by synaptic activity Recent advances in the understanding of the role of synaptic proteins in Alzheimer’s disease and other neurodegenerative disorders Synaptic and neuritic alterations during the progression of Alzheimer’s disease Diffuse plaques do not accentuate synapse loss in Alzheimer’s disease Robust central reduction of amyloid-β in humans with an orally available non-peptidic β-secretase inhibitor Clustering of plaques contributes to plaque growth in a mouse model of Alzheimer’s disease A reporter of local dendritic translocation shows plaque- related loss of neural system function in APP-transgenic mice Rapid appearance and local toxicity of amyloid-β plaques in a mouse model of Alzheimer’s disease plasticity and environmental enrichment: structural changes and neurotransmitter dynamics in several areas of the brain High-level neuronal expression of aβ1–42 in wild-type human amyloid protein precursor transgenic mice: synaptotoxicity without plaque formation Bidirectional activity-dependent morphological plasticity in hippocampal neurons CD33 and EPHA1 are associated with late-onset Alzheimer’s disease Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo Amyloid-β deposits lead to retinal degeneration in a mouse model of Alzheimer disease Astrocytic adenosine receptor A2A and Gs-coupled signaling regulate memory Synaptic pruning by microglia is necessary for normal brain development Microglia promote learning-dependent synapse formation through brain-derived neurotrophic factor BACE1 inhibition more effectively suppresses initiation than progression of β-amyloid pathology Prince, M. J. (2015). World Alzheimer Report 2015: The Global Impact of Dementia. Available online at: https://www.alz.co.uk/research/world-report-2015 [Accessed on August 29 Reducing endogenous tau ameliorates amyloid β-induced deficits in an Alzheimer’s disease mouse model Complement activation and β-amyloid-mediated neurotoxicity in Alzheimer’s disease Microglia sculpt postnatal neural circuits in an activity and complement-dependent manner Quantitative assessment of cortical synaptic density in Alzheimer’s disease Neuropathological alterations in Alzheimer disease Differential relationships of reactive astrocytes and microglia to fibrillar amyloid deposits in Alzheimer disease Natural oligomers of the Alzheimer amyloid-β protein induce reversible synapse loss by modulating an NMDA-type glutamate receptor-dependent signaling pathway Biochemical and immunohistochemical analysis of an Alzheimer’s disease mouse model reveals the presence of multiple cerebral Aβ assembly forms throughout life Amyloid-β protein dimers isolated directly from Alzheimer’s brains impair synaptic plasticity and memory Complement activation by neurofibrillary tangles in Alzheimer’s disease Molecular dissection of reactive astrogliosis and glial scar formation Dendritic spine abnormalities in amyloid precursor protein transgenic mice demonstrated by gene transfer and intravital multiphoton microscopy Impaired spine stability underlies plaque-related spine loss in an Alzheimer’s disease mouse model Tau pathophysiology in neurodegeneration: a tangled issue An English translation of Alzheimer’s 1907 paper “Über eine eigenartige Erkankung der Hirnrinde” The complement system: an unexpected role in synaptic pruning during development and disease The classical complement cascade mediates CNS synapse elimination Oligomerization of Alzheimer’s β-amyloid within processes and synapses of cultured neurons and brain Physical basis of cognitive alterations in Alzheimer’s disease: synapse loss is the major correlate of cognitive impairment Preparation of mouse brain tissue for immunoelectron microscopy Fibrillar amyloid deposition leads to local synaptic abnormalities and breakage of neuronal branches BACE1 inhibitor drugs in clinical trials for Alzheimer’s disease Naturally secreted oligomers of amyloid β protein potently inhibit hippocampal long-term potentiation in vivo Postsynaptic target specific synaptic dysfunctions in the CA3 area of BACE1 knockout mice Spatial relationships between astrocytes and classical plaque components TREK-1 and Best1 channels mediate fast and slow glutamate release in astrocytes upon GPCR activation Microglia: dynamic mediators of synapse development and plasticity Adult mouse astrocytes degrade amyloid-β in vitro and in situ Inflammation in Alzheimer disease-a brief review of the basic science and clinical literature Targeting the β secretase BACE1 for Alzheimer’s disease therapy Large soluble oligomers of amyloid β-protein from Alzheimer brain are far less neuroactive than the smaller oligomers to which they dissociate Deficient neuron-microglia signaling results in impaired functional brain connectivity and social behavior β-site amyloid precursor protein cleaving enzyme 1 levels become elevated in neurons around amyloid plaques: implications for Alzheimer’s disease pathogenesis Consequences of pharmacological BACE inhibition on synaptic structure and function Ziegler-Waldkirch Seed-induced Aβ deposition is modulated by microglia under environmental enrichment in a mouse model of Alzheimer’s disease Ziegler-Waldkirch Environmental enrichment reverses Aβ pathology during pregnancy in a mouse model of Alzheimer’s disease Citation: Ziegler-Waldkirch S and Meyer-Luehmann M (2018) The Role of Glial Cells and Synapse Loss in Mouse Models of Alzheimer’s Disease Received: 18 September 2018; Accepted: 20 November 2018; Published: 11 December 2018 Copyright © 2018 Ziegler-Waldkirch and Meyer-Luehmann. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) distribution or reproduction in other forums is permitted provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited in accordance with accepted academic practice distribution or reproduction is permitted which does not comply with these terms *Correspondence: Melanie Meyer-Luehmann, bWVsYW5pZS5tZXllci1sdWVobWFubkB1bmlrbGluaWstZnJlaWJ1cmcuZGU= Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher 94% of researchers rate our articles as excellent or goodLearn more about the work of our research integrity team to safeguard the quality of each article we publish How might President Trump's sweeping tariffs affect Maine and the nation We'll discuss potential impacts and what they may mean for Maine consumers businesses and key industries—from agriculture to the marine economy to tourism Panelists:Emily Blanchard, faculty fellow and associate professor of business administration, Tuck School of Business, Dartmouth College; former chief economist, U.S. Department of StateAndreas Waldkirch, professor of economics, Colby College Elizabeth Lasseter Waldkirch and Jonathan Thomas Wooten were wed on Saturday at First Presbyterian Church in Wilmington The bride was escorted by her father and given in marriage by her parents Bridesmaids were Jessica Howard of Jacksonville all of Wilmington; and Brad Tripp of Raleigh A reception at Pine Valley Country Club followed immediately after the service The couple are on a honeymoon cruise in the Western Caribbean The family of the groom hosted a barbecue for the wedding party and out-of-town guests on Friday night following the rehearsal Friends and family entertained the bride and her attendants with a luncheon on Friday at Cape Fear Country Club The bride is a music therapist at Broughton Hospital in Morganton The groom is an operations assistant for Overton's Inc NILES — Esther “Pat” Johnson the daughter of John and Irene Gilbert Patton She was employed as the head of the Officer’s Club at the U.S reading and spending time with her family and friends Esther will be deeply missed by granddaughter Tracy (Anthony) Raschilla of Girard; nephew David (Teddi) Agues of Bristol; and many great-nieces and great-nephews Kathleen Agues and Geraldine McFarland; and brother-in-law Family and friends may pay their respects to Esther on Saturday with a memorial Mass of Christian burial to be held at 10 a.m. The family asks all those attending the Mass and any visitation or calling hours Arrangements have been entrusted to the professional care of the Peter Rossi & Son Memorial Chapel donations can be made to her two favorite charities Family and friends may visit www.peterrossandsonfh.com to view this obituary sign the guest book and send condolences to Esther’s family | https://www.tribtoday.com | 240 Franklin Street SE [NoHo Arts District CA] – The world premiere of Dan Waldkirch’s side-splitting comedy ROD HINCKLEY BREAKS THE GUINNESS WORLD RECORD FOR LONGEST USABLE GOLF CLUB directed by Denise Devin heads to Zombie Joe’s Underground Theatre March 21-April 5 So he builds the world’s longest usable golf club And his adventure begins – one that will take him from the murky recesses of the Columbus Ohio Sewer to being hunted by secret “G” men to being hurtled into the Cosmos directed by Denise Devin and produced by Zombie Joe Dan Waldkirch is a playwright and musician from Wauwatosa His musical The Unsackable Man – a retelling of Moby Dick on an NFL football field – lit up ZJU audiences in 2022; Denise Devin has been Lead Director at ZJU for over 20 years writing and creating many firsts for the theater including ten children’s plays editing and directing a dozen Shakespeare plays working with many new plays from other playwrights and now stepping into a directorial role with the signature ZJU Urban Death Halloween Haunt Devin’s second collaboration with Dan Waldkirch Featuring the World’s Most Rib-Tickling Cast: Jonica Patella and Elif Savas AND the World’s Most Pluckiest Band Duo: Dan Waldkirch and Andrew Miller (Contains some strong adult language) Wheelchair Accessible Please adjust your search criteria and try again Donald Trump’s inauguration as the 47th US president on January 20 marks the beginning of four potentially unpredictable years for American — if not global — investment and trade.  Knowing that fDi’s worldwide readership has diverse takeaways from Mr Trump’s first term and 2024 campaign promises we’ve spent the past month gathering your predictions for what lies ahead as he returns to the White House.  The responses are telling. According to readers, an uptick in US foreign direct investment (FDI) and Elon Musk’s influence on more than just cost-cutting are on the cards while the president’s tariff threats will be part of his broader strategy as a “super negotiator”.  The majority (70%) of respondents expect Mr Trump’s policies to increase inbound FDI but the majority within that camp expect only a moderate uptick higher tariffs and the promise of deregulation are the most commonly cited drivers but this will be tempered by Trump’s “volatility” Investors’ desire for predictability is one reason why 19% of respondents expect inbound FDI to decrease as “the incoming administration is anything but predictable,” says Andreas Waldkirch in the US Another sceptic notes that Mr Trump’s “hostility to foreign business interests (other than his own) is significant”.  There is little consensus on his effect on outbound FDI. One-third of respondents expect a slight decrease, while 28% predict no impact at all. This reflects the broad alignment between Mr Trump and current president Joe Biden on reshoring and revitalising US industry, with less emphasis on spreading influence abroad For the 22% expecting outbound FDI to increase preservation of foreign market access in case of retaliation against Mr Trump’s trade policies is a big factor “US companies will have to invest more abroad to avoid the reciprocal tariffs on their exports that Trump will trigger,” says Charlie Robertson in the UK.  Mr Trump has promised to hike tariffs against China to 60% just 7% of respondents expect the president to follow through on this threat.  Most (54%) of them expect tariffs on just a handful of countries with the president using them as a bargaining tool “With his quintessential business-like thinking there would most likely be negotiations before tariffs are imposed,” says Adrian Tan in Singapore Others note his “tariff war will focus on selected products and services”.   Tariffs will continue to be a major determinant of Mr Trump’s relations with Beijing After overseeing the start of the US–China trade war in his first presidential term relations failed to improve under Mr Biden amid his China policymaking blitz.  More than three-quarters of you expect things to further deteriorate with just 22% of respondents expecting US–Sino relations to improve over the next four years. “Trump is US-centric and will not play nice … but China likely has more weight economically and can turn their back easier,” notes one respondent who expects relations to ‘drastically worsen’ Some believe Mr Trump’s China policy depends on whether he is influenced more by his advisor Elon Musk — given his huge operations in the country — or China hawks in his cabinet.  But readers are pessimistic about the Trump–Musk bromance with 43% expecting it to be over by mid-year Only 22% predict it will last the length of this White House term with clashing egos and Mr Musk’s “uncompromising” nature being the likely cause of relationship breakdown Several respondents also link the future of Inflation Reduction Act (IRA) incentives to the longevity of the Musk–Trump relationship the majority (56%) view is that IRA incentives will continue to run their course with no new green subsidies being offered “Trump has sold his soul to the petrochemical industry” and is “captive to conventional energy interests” 30% of respondents expect traditional energy to be among the biggest industry winners under the Trump presidency The next most-cited industry is manufacturing followed by aerospace and defence given the president’s desire “to strengthen US power” As for countries to reap trade and investment benefits under Mr Trump, the biggest winners are tipped to be Mexico and India. The former has the assurance of the US–Mexico–Canada trade agreement, although its scheduled renegotiation in 2026 engenders risks, and continued tariff-jumping by Chinese firms India will be buoyed by the “fraternal spirit” between Mr Trump and Narendra Modi Tied in third place are Canada and Vietnam which readers expect to benefit from firms diversifying production away from China to avoid US tariffs.  A more detailed analysis of the survey results will be in our next magazine Do you want more FDI stories delivered directly to your inbox? Subscribe to our newsletters. Make informed decisions, attract investment and stay competitive in the world of foreign and domestic direct investment. FirstBank has made a splash in the local construction lending market with the hiring of a seven-member team from BancorpSouth. Leading the group migrating to $2.3 billion FirstBank is Senior Vice President Bill Waldkirch, who for the past seven years led BancorpSouth's Franklin-based residential construction lending group. He is being joined by lenders Chuck Scruggs, Alex Hartz and Michael McClellan as well as portfolio manager Greg Templeton, office manager Nelda Vest and administrative assistant Brenda McKee. "Bringing this experienced group on board FirstBank immediately positions us as a leader in the Middle Tennessee residential lending market," said Chris Holmes, FirstBank's president and CEO. "It sends a clear signal that we are bullish on the Middle Tennessee economy and that we are establishing FirstBank to be a key player in the region's growth." The Waldkirch team will focus on lending to homebuilders and developers in Davidson, Williamson, Rutherford, Sumner, Wilson and Maury counties. "We look forward to bringing our experience and relationships to a bank with FirstBank's reputation and helping it continue growing in the Middle Tennessee market," said Waldkirch, who was a senior VP at Regions Bank before moving to BancorpSouth. "I have known many of FirstBank's executives for several years, and I'm thrilled that they are now my colleagues." Your browser is out of date and potentially vulnerable to security risks.We recommend switching to one of the following browsers: Lindsey Louise Waldkirch and Kevin Patrick Sullivan were wed on Saturday, July 19, at 5:00 p.m. at First Presbyterian Church in Wilmington, N.C. The Rev. Charles Dewitt Lee heard their vows. Music was provided by John Tablet, organist. Reader was Mark Double of Morgantown, W.Va. The bride is the daughter of Mr. and Mrs. Robert Bradley Waldkirch of Wilmington, and the granddaughter of Mr. and Mrs. Oscar Waldkirch, and Mrs. Thomas Wyatt Cobb and the late Mr. Cobb, all of Wilmington. The groom is the son of Mr. and Mrs. John Sullivan of Fairmont, W.Va., and the grandson of Mrs. Clyde Brookover and the late Mr. Brookover, and Mr. John Sullivan and the late Mrs. Sullivan, all of Fairmont, W.Va. The bride was escorted by her father and given in marriage by her parents. She chose her sister, Lasseter Waldkirch Wooten of Greenville, N.C., as her matron of honor. Bridesmaids were Elizabeth Hager, cousin of the bride of Winchester, Va., Meghan Bolling of Jamestown, N.Y., and Melissa Duckworth of Gassaway, W.Va. Sean Sullivan, brother of the groom of Fairmont, W.Va., served as best man. Groomsmen were Kyle Hoffman, cousin of the groom of Glade Springs, W.Va., Bryan Hamilton Smith of Fairmont, W.Va., and John James of Morgantown, W.Va. Ushers were Thomas Waldkirch, brother of the bride, and John Thomas Richardson, cousin of the bride, both of Wilmington, and Jonathan Wooten, brother-in-law of the bride, of Greenville, N.C. A reception at Pine Valley Country Club followed immediately after the service. The parents of the groom entertained the wedding party, family, and out-of-town guests with dinner at the Pilot House following the rehearsal Friday evening. Friends of the bride hosted the families and friends with a river cruise on the Winner Cruise Queen following the dinner. The bride and her attendants were guests at a bridesmaid luncheon on Wrightsville Beach on Friday. The couple are both music education graduates of the University of West Virginia and will make their home in Martinsburg, W.Va., following a honeymoon cruise. They both teach in the public schools of Hagerstown, Md.. Volume 16 - 2022 | https://doi.org/10.3389/fncel.2022.862918 This article is part of the Research TopicThe Role of Glial Cells in Neurodegenerative DiseasesView all 4 articles Alzheimer’s disease (AD) is characterized by the accumulation of amyloid-beta peptide (Aβ) and subsequent formation of the so-called Aβ plaques previous studies report white matter anomalies and corpus callosum (CC) atrophy in AD patients perturbations in the white matter can be observed years before expected disease onset suggesting that early stages of disease progression play a role in AD-associated loss of myelin integrity Through seed-induced deposition of Aβ we are able to examine alterations of central nervous system (CNS) integrity during the initial stages of plaque formation we investigate the impact of Aβ seeding in the CC utilizing various imaging techniques as well as quantitative gene expression analysis and demonstrate that Aβ deposits result in an imbalance of glial cells in the CC We found increased amounts of phagocytic microglia and reactive astrocytes while oligodendrocyte progenitor cell (OPC) numbers were reduced white matter aberrations adjacent to the Aβ seeding were observed together with an overall decline in callosal myelination This data indicate that the initial stages of plaque formation induce oligodendrocyte dysfunction which might ultimately lead to myelin loss Alzheimer’s disease (AD) is a neurodegenerative disorder during which patients suffer from progressing cognitive impairment and memory loss The most prominent neuropathological hallmarks are the extracellular aggregation of the amyloid-beta peptide (Aβ) in the form of amyloid plaques and intracellular neurofibrillary tangles While for decades the deposition of Aβ plaques is implicated with the onset of AD the abundance of Aβ-associated perturbations remains to be conclusively investigated reinforcing the relevance of (de-) myelination in the context of AD Strikingly, a magnetic resonance imaging (MRI) study from the Dominantly Inherited Alzheimer’s Network (DIAN) including autosomal dominant AD mutation carriers assessed white matter abnormalities years before expected disease onset (Lee et al., 2016) indicating the loss of myelin integrity at an early disease stage said white matter aberrations occurred simultaneously with decreasing Aβ42 levels in the CSF of mutation carriers implying a correlation between AD-associated demyelination and pre-symptomatic decline in CSF Aβ42 levels we report a pronounced astro- and microgliosis at the seeding site accompanied by a reduction of oligodendrocyte progenitor cells (OPCs) Aβ seeding led to myelin anomalies adjacent to the seeding area and MRI scans further revealed CC demyelination Our data thus provide one explanation for the reported early demyelination in AD patients by demonstrating that seed-induced diffuse Aβ can evoke the loss of white matter integrity before compact plaque formation All animal experiments were carried out in accordance with the policies of the state of Baden-Württemberg under the license number G16/060. We used heterozygous 5xFAD transgenic mice co-expressing human APPK670N/M671L (Sw) + I716V (Fl) + V717I (Lo) and PS1M146L + L286V under the control of the neuron-specific Thy-1 promoter (Oakley et al., 2006) We backcrossed heterozygous 5xFAD to C57BL/6 mice to generate heterozygous 5xFAD mice and wildtype (WT) littermates Only male mice were used in this study since female mice have a faster and earlier onset of Aβ plaque formation we wanted to minimize variability and reduce sample size Animals were group-housed under specific pathogen-free conditions 12-h dark cycle with food and water ad libitum Mouse brain homogenates were derived from 10-month-old plaque-bearing heterozygous 5xFAD transgenic mice and age-matched non-transgenic littermates Homogenates were obtained from the whole mouse brain Brain tissue samples were fresh-frozen and stored at −80°C until use Samples were homogenized in sterile phosphate-buffered saline (PBS) at 10% (w/v) and sonicated 3 × 5 s (30% amplitude The crude brain homogenate was centrifuged for 5 min (at 3,000 g 4°C) and the supernatant was stored at −80°C until use The animals were anesthetized via intraperitoneal injection with a mixture of ketamine (100 mg/kg body weight) and xylazine (5 mg/kg body weight) in saline. For bilateral stereotactic injections of brain homogenates, a Hamilton syringe was placed into the hippocampus (AP −2.3 mm; L 2.0 mm; DV −2.0 mm) of 7-week-old male 5xFAD and WT mice as described previously (Bachhuber et al., 2015) The animals were injected either with 5xFAD transgenic brain homogenate or WT brain homogenate (2.5 μl per hemisphere at an injection speed of 1.25 μl/min) or were left uninjected as control animals the needle was kept in place for an additional 2 min before it was slowly withdrawn The surgical site was cleaned with sterile saline and the incision was sutured Mice were monitored until recovery from anesthesia and incubated for 5 or 10 weeks Samples were separated by 4–12% NuPAGE Bis-Tris mini gels utilizing NuPAGE LDS sample buffer and NuPAGE MES SDS running buffer (Invitrogen) Proteins were transferred onto PVDF membranes (BioRad) and visualized using Clarity Western ECL Substrate (BioRad) The following peptides were used as standard and prepared (1:1 mixture) according to the manufacturer’s instructions: rPeptide Aβ1-40 HFIP (1 mg) (cat lot no.: 2061240H) and rPeptide Aβ1-42 HFIP (1 mg) ImageJ (National Institutes of Health freeware version 1.52a) was used for densitometric analysis Mice were transcardially perfused with 10 ml of ice-cold PBS followed by 10 ml of ice-cold 4% paraformaldehyde (PFA) in PBS (Roti-Histofix Brains were isolated and post-fixed in 4% PFA for 24 h followed by incubation in 30% sucrose (in PBS Frozen brains were cut into 25 μm thick coronal sections on a sliding microtome (SM2000R Sections were blocked in 5% normal goat serum (NGS) in PBS with 0.5% Triton X-100 Afterward slices incubated overnight at 4°C with antibodies diluted in PBS containing 1% NGS against the following: anti-Aβ (mouse Corresponding secondary antibodies conjugated to Alexa 488 or 555 (1:1,000) were used Sections were counterstained with DAPI (Sigma 1:10,000) and mounted with a fluorescence mounting medium (DAKO Fluorescence images of brain slices were taken using a Zeiss fluorescent microscope (Axio Imager M2M). Confocal micrographs were acquired with either an Olympus Fluoview FV1000 or a Zeiss LSM880 AiryScan confocal microscope. For analysis, every 10th brain section approx. 1 mm anterior and posterior to the injection site were immunostained, with an interval of 250 μm per section. The definition of the CC was based on the mouse brain atlas (Franklin and Paxinos, 2019) Total Aβ load was determined by calculating the relative areal fraction occupied with Aβ-positive staining (in %) in the CC using the image analysis software ImageJ (National Institutes of Health freeware immunoblot analysis of injected brain homogenates) About four to seven animals per group and four to five sections per animal were analyzed Cell number was quantified by counting the number of positively labeled cells in the CC of the animals This was mostly done in a semi-automated manner using custom-written macros Since GFAP exclusively stains the processes of reactive astrocytes the assessed cell number was substituted by the relative areal fraction occupied with the GFAP-positive signal (in %) six to seven animals per group and three to five sections per animal were analyzed All analyses were conducted in a blinded manner We collected the CC tissue and stored it immediately at −80°C the tissue was homogenized in RLT lysis buffer by mechanical dissociation (RNeasy Mini Kit RNA was isolated with the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol Reverse transcription and real-time PCR analysis were performed using the High-Capacity cDNA Reverse Transcription Kit and Gene Expression Master Mix reagents (Applied Biosystems) according to the manufacturer’s recommendations qPCRs were analyzed with a LightCycler 480 (Roche) we used the following TaqMan Gene Expression Assays: Actb (Mm01205647_g1) Mice were transcardially perfused with 20 ml of ice-cold PBS followed by 20 ml of ice-cold 4% PFA with 0.1% Glutaraldehyde in PBS Brains were isolated and post-fixed in 4% PFA + 0.1% Glutaraldehyde for 24 h 50 μm coronal sections of the CC were obtained using a vibratome (Leica VT1000 S) Sections were post-fixed in 1% OsO4 in 0.1 mM phosphate buffer (PB) for 40 min slices were dehydrated in an ascending ethanol series (50% 100%) and treated with propylene oxide (2 × 10 min) followed by a 1:1 mixture of propylene oxide and durcupan (1 h) and ultimately with pure durcupan (overnight) To initialize durcupan polymerization and finalization of the embedding procedure the tissue was placed in a 55°C oven for 48 h Ultrathin 40 nm thick sections of the region of interest were cut utilizing an ultramicrotome (Leica UC6 Reichert-Jung) before transfer onto a copper slot grid for transmission electron microscopy (TEM) (Philips CM100) the axonal diameter and the corresponding diameter of axon + myelin sheath were measured using ImageJ the diameter of the axon was divided by the entire fiber (g-ratio = diameter of the axon/diameter axon + myelin sheath) two g-ratios were assessed (orthogonally to each other) and the mean was determined About 5 and 10 weeks post-intracerebral stereotactic injections MRI experiments were performed while continuously monitoring body temperature and respiratory rate for maintenance of constant physiological levels (body temperature: 35.5 ± 1.5°C; respiratory rate: 50–60 breaths/min) For the morphological respiratory-gated T2-weighted and diffusion tensor imaging (DTI) acquisitions anesthesia was induced with 4.0% and maintained with 1.5% isoflurane (Forene; Abbvie Deutschland GmbH & Co The total scanning time ranged from 45 to 50 min animals were allowed to spontaneously recover High-resolution T2-weighted morphological images were acquired using a RARE sequence (TE/TR = 40 ms/4,000 ms) An acquisition matrix of 256 × 196 and a FOV of 1.3 × 1 cm2 led to a spatial resolution of 0.051 × 0.051 × 0.3 mm3 30 unique sampling orientations are more than sufficient for a robust estimation of anisotropy and tensor orientation The method used the continuity information contained in the fibers reconstructed during the global tracking procedure to introduce subvoxel information based on supporting information from neighboring voxels After the generation of the sufficient number of fibers passing a voxel at different spatial locations their density was used as intravoxel information to construct the FD map The directionality of the fibers was incorporated into the FD by assigning red/green/blue color to different spatial directions: red: mediolateral and blue: rostrocaudal orientation; thus generating highly resolved spatial histograms of diffusion orientations referred to as high-resolution fiber map GraphPad Prism 6 was used for statistical analysis Data sets were tested for normality (D’Agostino-Pearson Omnibus K2 normality test; significance level p = 0.05) before employing the appropriate parametric or non-parametric statistical comparison test Applied statistical tests are depicted in the respective figure legends Reported values represent the means ± SEM Significance level α was set to 0.05 *p < 0.05; **p < 0.01; ***p < 0.001 Intrahippocampal injections of amyloid-beta peptide (Aβ)-containing brain homogenate induces Aβ seeding in the corpus callosum (CC) (A) Timeline for the Aβ seeding experiments (B) Representative immunoblot from whole brain extracts of aged 5xFAD and WT mice compared to synthetic (synth.) Aβ (mixture of synthetic Aβ1-40 and Aβ1-42) Immunoblot was probed with the 6E10 antibody (C) Illustration of the region of interest (CC; indicated in red) used for subsequent image analysis (D) Fluorescence microscopy visualizing Aβ-material (6E10; red) of 4-month-old WT and 5xFAD mice which were either uninjected (uninj.) or injected with Aβ-containing brain homogenate of an aged 5xFAD mouse (+5xFAD hom.) Dashed line indicates the Aβ seeding area (E) Quantification of the Aβ load relative to the CC n = 6–7 mice with n = 5 sections analyzed per animal Each symbol represents data from one animal Kruskal–Wallis test followed by Dunn’s multiple comparison test Enhanced microglia density and phagocytic activity in the corpus callosum (CC) of amyloid-beta peptide (Aβ)-seeded mice (A) Immunofluorescent Iba1 (green) and 6E10 (red) staining of 4-month-old WT and 5xFAD mice which were either uninjected or injected with 5xFAD brain homogenate Scale bar for overview and inset 100 μm (B) Respective quantification of Iba1-positive cells in the CC n = 6–7 mice with n = 4–5 sections analyzed per animal Each symbol represents data from one mouse (C) Quantification of the ratio between Iba1 single-positive and Iba1 and CD68 double-positive cells in the CC of 5xFAD animals either uninjected or injected with either WT or 5xFAD homogenate n = 4–5 mice with n = 5 sections analyzed per animal two-way ANOVA followed by Tukey’s multiple comparison test ****p < 0.0001 either not injected or injected with 5xFAD brain extract Arrowheads indicate microglial CD68 expression adjacent to Aβ deposits as a response to the seed-induced Aβ material pro-inflammatory cytokine expression was enhanced and microglia as well as astrocytes became activated Reactive astrogliosis in the corpus callosum (CC) adjacent to the amyloid-beta peptide (Aβ) seeding (A) Representative fluorescence micrographs of GFAP (green) and 6E10 (red) of 5xFAD brain homogenate-injected or uninjected 4-month-old WT and 5xFAD mice (B) Analysis of the GFAP signal relative to the CC area (C) Pearson correlation plots of the amount of Aβ seeding (%) relative to GFAP signal (%) in 5xFAD mice injected with 5xFAD homogenate Each symbol represents data from one section and each color represents one mouse (D) RT-qPCR of the astrocyte-associated genes Gfap and Emp1 in 5xFAD mice injected with either WT (n = 5) or 5xFAD (n = 5) homogenate relative to 5xFAD uninjected mice (n = 5) Data were normalized to the housekeeping gene Actb unpaired non-parametric t-test (Mann–Whitney) (E) Gene expression of the chemokines Cxcl10 and Ccl5 as well as Apoe in 5xFAD animals with WT (n = 5) or 5xFAD (n = 5) homogenate relative to uninjected 5xFAD mice (n = 5) (F) Quantification of the ratio between GFAP signal and GFAP and C3 co-localizing signal in the CC of 5xFAD animals either uninjected or injected with WT or 5xFAD homogenate n = 4–6 mice with n = 4–5 sections analyzed per animal Reduced oligodendrocyte progenitor cell (OPC) number and disrupted white matter integrity in the corpus callosum (CC) exhibiting amyloid-beta peptide (Aβ) deposits (A) Representative immunofluorescent staining of PDGFRα (green) and Aβ (red) of 4-month-old WT and 5xFAD mice (B) Respective analysis of PDGFRα-positive cells in the CC n = 6–7 mice analyzing n = 4–5 sections per animal (C) Confocal microscopy of MBP (green) and Aβ (red) of 4-month-old 5xFAD mouse injected with 5xFAD brain homogenate Dashed line indicates focal absence of MBP signal (D) Graphic representation of the region of interest (indicated as a white line) in the CC (red) used to quantify the CC thickness (E) Fluorescence microscopy visualizing MBP (green) and Aβ (red) of 4-month-old WT and 5xFAD mice (F) Respective quantification of the CC thickness n = 6–7 mice with n = 3–5 sections analyzed per animal Kruskal–Wallis test followed by Dunn’s multiple comparison test was used comparing the Aβ seeded animals to the other 5xFAD groups indicating reduced axonal myelination in those animals We hence recapitulate that seed-induced Aβ deposits in 5xFAD transgenic mice elicited diminished OPC levels concomitant with a reduction in callosal thickness and myelin aberrations in the white matter leading to the conclusion that induced Aβ seeding in the CC of 5xFAD animals can interfere with white matter integrity Seeding-associated demyelination in the corpus callosum (CC) (A) Timeline for the amyloid-beta peptide (Aβ) seeding experiments including the performed magnetic resonance imaging (MRI) scans 5 and 10 weeks post-injection (p.i.) (B) Representative fluorescent micrographs of the Aβ signal (red) of 4-month-old 5xFAD mice 5 and 10 weeks after the intrahippocampal inoculation of Aβ-containing brain homogenate (C) Analysis of the Aβ load relative to the CC n = 4–7 mice with n = 5 sections analyzed per animal (D) Representative visualization of the fiber density (FD) and high-resolution fiber map of 4-month-old 5xFAD mice 10 weeks p.i with age-matched WT homogenate or 5xFAD homogenate and an uninjected control (E) Respective analysis of the callosal fractional anisotropy (F) Comparison of the fractional anisotropy from seeded 5xFAD mice 5 and 10 weeks p.i n = 6 mice; Each symbol represents data from one animal corroborating the relevance of myelin formation in an Alzheimer’s disease context and suggesting a potential beneficial effect of pro-myelinating strategies for patients suffering from AD Although human studies would be indispensable to evaluate the downstream effects of demyelination in AD the present study reiterates the harmful consequences of Aβ accumulation on myelin integrity already at an early disease stage Since our seeding model resembles the early stages of Aβ plaque deposition we hypothesize that the Aβ seed-induced reduction in OPCs reflects the early stages of AD-associated alteration in oligodendrocyte dynamics Although further examination of our seeding model would be essential to solidify our hypothesis our data so far indicate myelin aberrations as a consequence of seed-induced Aβ deposits Additional indication for the activation of glial cells upon callosal Aβ seeding is the expression of pro-inflammatory cytokines important to mention that the experimental setup does not allow to distinguish the origin of chemokine overexpression diffuse Aβ material can evoke focal myelin degeneration before dense-core plaque formation Aβ seeding led to significant callosal atrophy and MRI scans confirmed disturbed myelin integrity of the white matter these white matter aberrations were not detectable in the first scan 5 weeks p.i. indicating that the white matter aberrations are linked to Aβ seeding this study demonstrates that seed-induced Aβ deposits in the CC of 5xFAD mice provoke pronounced reactive astrogliosis and increased numbers of phagocytic microglia focal demyelination adjacent to the Aβ material and overall corruption of myelin integrity in the white matter was visible More efforts will be crucial to comprehend the impact of Aβ-associated white matter abnormalities and demyelination as well as to evaluate pro-myelinating approaches to combat myelin loss our data provides valuable insights into the glial response during the early phase of Aβ pathogenesis in the white matter The original contributions presented in the study are included in the article/Supplementary material further inquiries can be directed to the corresponding author The animal study was reviewed and approved by the State of Baden-Württemberg VA and MM-L conceived and planned the experiments VA contributed to all aspects of the experiments and data analysis DL and OK performed electron microscopy experiments WR and DvE provided expertise in diffusion tensor imaging and magnetic resonance imaging WR performed the MRI scans and data analysis MM-L supervised the project and coordinated the study VA was supported by the Fill in the Gap fellowship (Medical Faculty Freiburg) MM-L was supported by the German Research Foundation (DFG) (ME3542/2-1 to MM-L) and the Alzheimer Forschung Initiative e.v We thank the Lighthouse Core Facility staff of the Medical Center University of Freiburg for their help with confocal microscopy resources and excellent support We thank the Core Facility Preclinical Imaging Research Center (AMIR) Department of Radiology – Medical Physics of the University Hospital Freiburg for support in acquisition (i.a All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fncel.2022.862918/full#supplementary-material Supplementary Figure 1 | Myelin aberrations and reduced g-ratio in the corpus callosum of Aβ-seeded 5xFAD mice (A) Electron microscopy of the corpus callosum from 4-month-old WT and 5xFAD mice which were either uninjected or injected with either age-matched WT or 5xFAD brain homogenate Examples for myelin alterations such as excess cytoplasm in the inner loop (arrow) demyelinated axons (arrowhead) and myelin compaction deficits (asterisk) are indicated (B) Quantification of white matter integrity by assessing the ratio of intact and aberrant myelin (in percent) Changes visible in the Aβ seeded 5xFAD group were not significant compared to the other groups (C) g-ratio analysis of the electron micrographs n = 2 mice with n = 184–329 measured axons per animal Kruskal–Wallis test followed by Dunn’s multiple comparison test was utilized comparing the Aβ seeded animals to the others groups Supplementary Figure 2 | No evident changes in white matter myelination when Aβ seeding is still absent (A) Visualization of the fiber density and high-resolution fiber map of 5xFAD mice 5 weeks post-injection of age-matched WT homogenate (+WT hom.) or 5xFAD homogenate and an uninjected control (B) Respective analysis of the callosal fractional anisotropy Inhibition of amyloid-β plaque formation by α-synuclein Dynamic changes in myelin aberrations and oligodendrocyte generation in chronic amyloidosis in mice and men Super-resolution track-density imaging studies of mouse brain: Comparison to histology Enhancing myelin renewal reverses cognitive dysfunction in a murine model of Alzheimer’s disease Microglia contribute to the propagation of Aβ into unaffected brain tissue Induction of cerebral β-amyloidosis: Intracerebral versus systemic Aβ inoculation Google Scholar Quantitative evaluation of 10 tractography algorithms on a realistic diffusion MR phantom Paxinos and Franklin’s the mouse brain in stereotaxic coordinates Google Scholar Remyelination in the CNS: From biology to therapy Aβ seeding as a tool to study cerebral amyloidosis and associated pathology Distinct Aβ pathology in the olfactory bulb and olfactory deficits in a mouse model of Aβ and α-syn co-pathology Methotrexate chemotherapy induces persistent tri-glial dysregulation that underlies chemotherapy-related cognitive impairment Microglia contribute to normal myelinogenesis and to oligodendrocyte progenitor maintenance during adulthood Corpus callosum atrophy is a possible indicator of region– and cell type–specific neuronal degeneration in Alzheimer disease: A magnetic resonance imaging analysis Mapping remodeling of thalamocortical projections in the living reeler mouse brain by diffusion tractography Amyloid β1–42 oligomer inhibits myelin sheet formation in vitro The connectomics of brain demyelination: Functional and structural patterns in the cuprizone mouse model The effect of gradient sampling schemes on measures derived from diffusion tensor MRI: A Monte Carlo study Self-propagation of pathogenic protein aggregates in neurodegenerative diseases Evidence for seeding of β-Amyloid by intracerebral infusion of Alzheimer brain extracts in β-Amyloid precursor protein-transgenic mice Aβ oligomers trigger and accelerate Aβ seeding A Unique microglia type associated with restricting development of Alzheimer’s disease White matter hyperintensities are a core feature of Alzheimer’s disease: Evidence from the dominantly inherited Alzheimer network: White Matter hyperintensities in familial AD Developmental heterogeneity of microglia and brain myeloid cells revealed by deep single-cell RNA sequencing Astrocyte-microglia cross talk through complement activation modulates amyloid pathology in mouse models of Alzheimer’s disease Activated microglia drive demyelination via CSF1R signaling Single-cell transcriptomic analysis of Alzheimer’s disease Motor skill learning requires active central myelination Myeloid Cells in Alzheimer’s disease: Culprits Exogenous induction of cerebral ß-amyloidogenesis is governed by agent and host Focal demyelination in Alzheimer’s disease and transgenic mouse models White matter changes in Alzheimer’s disease: A focus on myelin and oligodendrocytes Fractalkine-dependent microglial pruning of viable oligodendrocyte progenitor cells regulates myelination and neuron loss in transgenic mice with five familial Alzheimer’s disease mutations: Potential factors in amyloid plaque formation Preservation of a remote fear memory requires new myelin formation Loss of TREM2 function increases amyloid seeding but reduces plaque-associated ApoE Effects of aging on myelinated nerve fibers in monkey primary visual cortex Google Scholar Global fiber reconstruction becomes practical PDGFRA/NG2 glia generate myelinating oligodendrocytes and piriform projection neurons in adult mice Cdc42 and Rac1 signaling are both required for and act synergistically in the correct formation of myelin sheaths in the CNS Myelin degeneration and diminished myelin renewal contribute to age-related deficits in memory Ziegler-Waldkirch Seed-induced Aβ deposition is modulated by microglia under environmental enrichment in a mouse model of Alzheimer’s disease Ziegler-Waldkirch deposition alters neuronal function and impairs olfaction in a mouse model of Alzheimer’s disease Psychiatry doi: 10.1038/s41380-022-01686-5 von Elverfeldt D and Meyer-Luehmann M (2022) Seed-induced Aβ deposits in the corpus callosum disrupt white matter integrity in a mouse model of Alzheimer’s disease Copyright © 2022 Aires, Ziegler-Waldkirch, Friesen, Reichardt, Erny, Loreth, Harborne, Kretz, von Elverfeldt and Meyer-Luehmann. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) Glimpses of the Past: March 8-14USA TODAY NETWORK-WisconsinMarch 8 1983A citizen committee will be appointed by the Green Bay Plan Commission to review controversial billboard restrictions proposed by the city’s planning staff Commissioners decided Monday to create the citizen panel — including sign company representatives — to iron out problems between the sign industry and the city Committee members will be selected by the commission at a later date The City Council last week refused to adopt a set of restrictions banning new billboards from the central city and imposing other controls on the placement of the signs Commissioners have wrestled with the issue since Dec 13. Planners suggested the downtown sign ban to protect a $55 million public and private redevelopment investment form visual clutter Other curbs were designed to keep the signs away from waterfronts Annexation of a 31-acre tract in Bellevue to the Green Bay Metropolitan Sewerage District was opposed by De Pere Alderwoman Joan Mills at an MSD Commission hearing Monday Proposed for residential development and including an 11-acre park the tract is located along Town Hall Road near the proposed Interstate 43 Bellevue interchange Mills contended that there is no demonstrated need for the annexation stating that Bellevue already has plenty of land within the sewerage district which has not been developed. She noted also that the petitioning area is not part of the Brown County comprehensive plan for residential development 1947Photography’s growing popularity became evident Sunday afternoon as 200 visitors one of the largest crowds that ever viewed an opening attended the second annual exhibition of the Green Bay Camera club in the north gallery of the Neville Public Museum The collection of 97 prints by 16 exhibitors was divided into 12 classifications with prizes awarded in each group by the judges won the grand prize for “Pals,” a poetic landscape.  Ralph Pratt won the most ribbons with three firsts and four honorable mentions Development of a modern shopping center in De Pere is in prospect with the announcement of the sale of two well-known business properties to Dr Waldkirch has purchased the Lee Brothers Company store at 124 N Broadway and the James Street Garage property at Wisconsin and James Streets formerly owned and operated by Peter Van Ooyen Transfer of the two properties to new ownership constitutes one of the largest transactions in De Pere’s commercial history The new owner contemplates improvement of the store properties and enlargement of stocks and facilities so as to provide a modern shopping center to serve the needs of De Pere and surrounding territory Some of Saddam Hussein’s most helpless victims — the sick and disabled — are getting relief from one of Green Bay’s Army Reserve units Volunteers from the 432nd Civil Affairs Company are washing windows and feeding patients at the Handicapped Care Home in the al-Omariya suburb of Kuwait City Most of the 125-soldier company arrived in Saudi Arabia in mid-January after being called to active duty Jan 3. Eighteen members were called up in early December and were in Saudi Arabia by Dec most of the hospital’s 250 staff fled 18 nurses and a few Palestinian volunteers to care for 850 patients.  Enter the 432nd. A civil affairs company’s job is to help restore services to a war-torn area. It appeared that the services of the Green Bay area soldiers were needed here more than anywhere else Would You Pay 50 cents to keep the Packers Or … Are the Packers worth a couple of packs of smokes a half-pound of coffee or any other four-bit item…to you The figure seems rather fantastic when you place it alongside the price of a new stadium co-chairman of the Stadium Ward committee along with Councilman Roman Denissen explained it at an informal meeting of city councilmen Clarence Nier and Packer officials at the Northland Hotel last night: “The City of Green Bay’s cost in building the stadium will be approximately $600,000 in 20 years — the original $480,000 plus interest Our population should average 60,000 over the next 20 years and probably will go higher the average cost per person for the city’s share would be $10 for 20 years.” Citizens of Green Bay will vote April 3 on a referendum to decide whether or not the City of Green Bay should issue $960,000 in bonds for the construction of a new stadium 1991Mandatory curbside recycling could come to one-third of Green Bay homes by July The department of public works has recommended the city start phasing in a curbside recycling program in the summer Two-thirds of residences would be required to separate recyclables from trash by the end of the year. All residences would take part by April 1992 The proposal calls for residents to put recyclables into an 18-gallon bin to be provided by the city. Materials to be mixed within the bin would include glass The city expects to recycle 7,033 tons of material annually based on results of the city of Milwaukee recycling program. That would be 16 percent of city refuse taken to landfills in 1990 Full Screen1 / 9Previous photoNext photoKit Kats from Japan are displayed for sale at Economy Candy in New York's Lower East Side (AP Photo/Richard Drew)Kit Kats from Japan are displayed for sale at Economy Candy in New York's Lower East Side (AP Photo/Richard Drew)Snickers bars from Brazil are displayed for sale at Economy Candy in New York's Lower East Side (AP Photo/Richard Drew)A shopper looks at Haribo candies from Germany at Economy Candy on New York's Lower East Side (AP Photo/Richard Drew)A shopper chooses Haribo candy from Germany (AP Photo/Richard Drew)Milka chocolate bars from Germany are displayed for sale at Economy Candy in New York's Lower East Side (AP Photo/Richard Drew)A shopper at Economy Candy chooses Skittles from Japan (AP Photo/Richard Drew)Candies from around the world are displayed for sale at Economy Candy in New York's Lower East Side (AP Photo/Richard Drew)Shoppers visit Economy Candy in New York's Lower East Side (AP Photo/Richard Drew)Copyright 2025 The Associated Press Kit Kats from Japan are displayed for sale at Economy Candy in New York's Lower East Side NEW YORK – Economy Candy’s shelves brim with sweets from around the world – gummies from Germany chocolates from Japan and a panoply of candies from across the U.S Standing amid it all, columns of bright jellybeans to his left and exotic Kit Kats to his right, owner Mitchell Cohen is quick with his assessment of how many of this shop’s 2,000-plus items are affected by the historic round of tariffs announced by President Donald Trump “I think all of them,” Cohen says at his store on New York's Lower East Side Few corners of the American economy are untouched by the sweeping tariffs being imposed by Trump Cohen had just begun to feel a barrage of inflation-driven price increases from suppliers ease when the tariff threats arrived. For a business with a name like Economy Candy, he wants to remain affordable but fears how high some prices may have to climb in the coming months “I think it’s gonna be another round of this hyperinflation on some items,” says 39-year-old Cohen Stepping into Economy Candy feels like a time warp Its name is emblazoned on a sign in a vintage and crossing below its green-and-white striped awning butterscotches and Lemonheads in the front window oldies music sounds overhead and customers mill around stacks of candy bars they forgot still existed It represents just a blip in the country’s $54 billion candy industry But it was already feeling the weight of surges in prices of cocoa and other ingredients before tariffs were layered on Candy and gum prices are up about 34% from five years ago and 89% from 2005, according to Consumer Price Index data. Price, according to the National Confectioners Association, has become the top factor in consumers’ candy purchase decisions About a third of Economy Candy’s products are imported crowded on shelves and tables near the store’s rear There aren’t just “more German Haribo varieties than the Haribo store in Germany,” as Cohen claims They have every Milka bar they can find in Switzerland every type of Leone hard candies that Italy churns out and as many exotic Kit Kats from Japan as they can fit while passion fruit mousse Snickers are from Portugal But even an American-made Snickers isn’t immune While the bars may roll off conveyors in Texas, they rely on ingredients from around the globe says Snickers bars include chocolate from Guyana and sugar from Brazil and are wrapped in packaging from Canada All are now subjected to varying levels of tariffs “There’s a lot of ingredients in there that have to come from other countries,” says Andreas Waldkirch an economics professor at Colby College who teaches a class on international trade “Unless you’re talking about something very simple from your local farmers market almost every product relies on ingredients from elsewhere Those indirect costs are really what’s going to drive up prices.” The story repeats with American candies across the store – the boxes of Nerds and bags of Sugar Babies and rolls of Smarties are all inextricably tied to the global supply chain A table teeming with those domestic delicacies takes center stage near Economy Candy’s entrance Cohen took over the store from his parents who took it over from their parents before everything on the store’s centerpiece table of American treats cost 59 cents but customers who bought a whole box paid a discounted rate of $1 per piece Cohen can’t even get them wholesale at that price Cohen calls the selection a “loss leader” but thinks it's important to showcase his store's affordability he’s not sure he’ll be able to put off price increases “When your margins are coming down and your dollar doesn’t go as far at the end of the day “But I don’t want anyone to come into Economy Candy and not think that it’s economical.” The biggest-ticket implications of the tariff blitz understandably gain the most attention – the thousands of dollars a car’s price tag may grow the tens of thousands that disappear from a retirement account in a single day But here among the root beer barrels and licorice strands you're reminded that small-dollar items are affected too the business Cohen’s grandfather started focused on shoe and hat repairs when few in a neighborhood of crowded tenements had money for such fixes business hasn't always been Chuckles and Zagnuts 11 attacks kept tourists away and had sales sagging and the pandemic closed the store and forced it to pivot to online sales He sells products that aren’t made in America and he sells American products made with ingredients from across the globe He had just been making headway on beginning international sales but the web of tariff rules may make it impossible tariff could rise to nearly 25% if the import taxes Trump put on goods from dozens of countries are fully implemented Wednesday That would be the highest rate in more than a century including tariffs widely blamed for worsening the Great Depression Trump said imposing the tariffs amounted to a “liberation day” for a country that has been “looted raped and plundered” by friend and foe alike Cohen isn't sure how that can be true for a business like his “I can understand bringing manufacturing and bringing things back to America we rely on raw materials that just aren’t native to our country,” he says “And it’s not like I can get a green tea Japanese Kit Kat from an American company.” As Cohen stood before mounds of strawberry candies in shiny wrappers and little cubes of caramel in cellophane the first word of the tariff’s concrete impact on him arrived A French supplier emailed saying it was immediately imposing a 5% surcharge due to the tariffs expressing regret for the move and hope that “the situation will be resolved swiftly.” He wants this to be a happy place for visitors “You travel back to a time when nothing mattered,” Cohen says Matt Sedensky can be reached at msedensky@ap.org and https://x.com/sedensky rewritten or redistributed without permission TV Listings Email Newsletters RSS Feeds Closed Captioning / Audio Description Contact Us Careers at WPLG Terms of Use Privacy Policy Public File FCC Applications EEO Report Do Not Sell My Info 1.0 Host Exhibit Copyright © 2025 Local10.com is published by WPLG INC., a Berkshire Hathaway company. Join the conversation You can save this article by registering for free here. Or sign-in if you have an account A group of Vancouver residents gathered in Douglas Park on Saturday to urge city council to ease up on what’s required for daycare operators to break ground in new spaces Subscribe now to read the latest news in your city and across Canada Create an account or sign in to continue with your reading experience Peter Waldkirch joined members of Abundant Housing Vancouver an advocacy group that typically raises the issue of the city’s lack of affordable housing “It is the same sort of broken municipal systems that led to our city’s housing crisis that’s also causing this shortage of child care spots,” said Waldkirch “Licensing regulations are unnecessarily strict and Vancouver is stuck in a cycle of tremendous inertia when it comes to political leaders pushing forth any solutions.” Stay on top of the latest real estate news and home design trends By signing up you consent to receive the above newsletter from Postmedia Network Inc The next issue of Westcoast Homes will soon be in your inbox Interested in more newsletters? Browse here. After a refusal in May due to objections mostly related to parking McCormick appealed to the board of variance but the licence was struck down a second time this month City representatives said the decision was not based on concerns about the daycare’s operator or facility but “It just doesn’t make sense for Vancouver to be turning down daycare spots because of neighbours’ noise complaints during a housing and childcare crisis,” Waldkirch said “I understand that change in your neighbourhood can be difficult to embrace when you buy a home in a wealthy neighbourhood you’re not buying the neighbourhood itself.” Waldkirch is among those pushing the city to expedite licensing processes for child care spaces by reducing what’s required for approval “Requirements to open up a daycare in Vancouver are much stricter than in B.C.,” he said children enrolled younger than four must get two to three hours of direct sunlight every day — but even Health Canada doesn’t advise this.” She expects a staff report on the matter later this year Vancouver currently has an estimated shortage of almost 15,000 licensed child-care spaces saying they are impeding housing construction and said he has introduced legislation aimed at ensuring municipalities approve enough homes “Where community members complain that eight more children will make noise and we can’t have it in our community transmission or republication strictly prohibited This website uses cookies to personalize your content (including ads), and allows us to analyze our traffic. Read more about cookies here. By continuing to use our site, you agree to our Terms of Use and Privacy Policy You can manage saved articles in your account A Nashville neighborhood will retrace its roots Saturday thanks to a connection made between one of the area's newest homeowners and one of its founding families named after the matron of the family that created a 60-home subdivision in the early 1900s — will stand for historic preservation While she didn't at first know the home's significance Rose had a hunch she should look into its history 'If only these walls could talk,' " she said But Rose didn't have to rely on the walls. She tracked down 91-year-old William Waldkirch II, son of the man who developed homes on three blocks and whose family name still appears on property deeds identifying the Waldkirch Subdivision The history testifies to a much different era of development a time when the European immigrant family subdivided its 18 acres but still built several homes for relatives nearby — an "unwavering love and family commitment," Rose said Waldkirch and Rose will place a bronze plaque at the home at 11 a.m. Saturday naming it "Caroline House" in honor of the man's grandmother. It also will kick off the neighborhood's annual home tour which features historic homes and raises money for local beautification Rose bought the home a year ago while away on vacation, acting quickly based only on pictures. But she pursued more information at the Tennessee State Library and Archives, eventually filling in the Waldkirch family history back to the end of the Civil War she'd take her findings to William Waldkirch who was born in 1923 in the home next to hers "He would fill in the gaps for me," she said The large family arrived in Nashville by 1867 and eventually developed Lawrence Waldkirch and — what is now — Bradford avenues But Bradford was initially Caroline Avenue until about 1941 when the city changed it to match the stretch that extends to the east Waldkirch last visited his childhood street during a family reunion about 10 years ago The driveway he remembered as being steep as a child — like the Big Dipper ride at the fairgrounds — seemed rather normal away from a place where extended families live together "is the people who are there now have kept those places beautifully." Reach Tony Gonzalez at 615-259-8089 and on Twitter @tgonzalez The 12th annual neighborhood home tour includes seven houses and one garden Proceeds benefit the nonprofit 12South Neighborhood Association and its alley cleanups Saturday; the commemorative ceremony at 906 Bradford Ave Tickets: $10 in advance at www.12southtour.com or $12 that day available in the parking lot of 12South Yoga at 2814 12th Ave An external investigation has cleared an NPA Vancouver city councillor of conflict of interest Lawyer Henry Wood dismissed pro-density activist Peter Waldkirch’s Jan finding no evidence that the 2018-elected councillor used her PlaceSpeak.com civic engagement company improperly Waldkirch’s complaint to city manager Sadhu Johnston took issue with a page on the PlaceSpeak website showing Hardwick and an image of city hall with the headline “You don’t need to wait for an election to have your voice heard – I’m listening.” Waldkirch alleged that Hardwick was confusing citizens and using public office to benefit her private company “I do not believe it is appropriate for her to use it for city business and there is at least the appearance of this,” Waldkirch wrote in the email to Johnston just over 30 minutes after his initial complaint to Johnston He publicly accused Hardwick of “attempted privatization” of public engagement and called PlaceSpeak a “black box.” Waldkirch’s social media messaging aligns with Abundant Housing Vancouver the coalition of Liberal and NDP activists lobbying city council to rubber-stamp rezonings of single-family houses to make way for townhouses and apartment buildings AHV associates have frequently targeted Hardwick on social media and in newspaper commentaries since her election on a platform favouring a citywide plan and better consultation with neighbourhoods In his May 5 report to Mayor Kennedy Stewart Wood found no evidence of any contravention of the City of Vancouver Code of Conduct or Vancouver Charter There was nothing to contradict Hardwick’s assertions that she will give due consideration to all public input and that the data architecture used by PlaceSpeak ensures the privacy of respondents’ personal data Whether the website presentation implies official city status is beyond city hall’s code and charter As for Hardwick’s financial interest in PlaceSpeak Wood concluded that Waldkirch’s concern over financial gain was “prospective and speculative.” “We are dealing with a soft launch of a consultation site on which the only question posed was ‘Do you think that you are being heard by the City of Vancouver?’ There is no obvious potential conflict arising from the substance of that issue,” Wood wrote Also in Hardwick’s favour was her purpose for the initial exposure of the page: she was seeking input on potential conflict of interest concerns from the city’s director of legal services The page in question was taken down after Waldkirch complained and not reactivated during Wood’s investigation Wood offered a “final cautionary note,” after seeing a page on PlaceSpeak seeking feedback on the Broadway Subway extension to the University of B.C He suggested there is a risk that if PlaceSpeak hosts a topic of significant interest in Vancouver that “the mythical reasonable elector might become concerned if Coun Hardwick were to vote in support of that proponent’s position and that he/she might conclude that her motivation was tainted by the business relationship.” Hardwick might choose to declare an apparent conflict and withdraw from discussion or voting Wood also wrote that constituents expect their chosen representatives to avoid potential conflicts so that they can fully participate and vote on matters of civic performance Stewart’s May 13 letter accepted Wood’s dismissal of the complaint and deemed the matter closed she said she is “happy to let the report speak for itself.” Stewart apparently took Wood’s report as a signal that he could embark on his own type of digital engagement separate from official city hall communications channels Stewart sent invitations to those on his 2018 campaign email list to join a new “Van-News Team email group” so he could seek public input advice and support from both Vancouverites and non-Vancouverites you will receive a single monthly email update and up to three invitations every four weeks to attend events take part in online actions or other activities that will help us build a Vancouver that works for everyone,” said the June 6 email appeal attendees of the civic-supported Khatsahlano Street Party on West 4th Avenue noticed posters urging citizens to fill-out a questionnaire on Stewart’s campaign website The posters recycled a photograph from independent Stewart’s 2018 union-supported mayoral campaign and read: “Have Your Say on Housing: Mayor Kennedy Stewart wants to hear from you.” The posters did not include any of Stewart’s city hall contact information Stewart’s posters and website are a common tactic used by politicians to build or update voter and donor-targeting databases a digital ad agency that also worked on his 2015 federal NDP campaign in Burnaby South The next scheduled civic election is three-and-a-quarter years away Support theBreaker.news for as low as $2 a month on Patreon. Find out how. Click here. theBreaker.news and theBreaker.news Podcast: your source for news opinion and analysis about British Columbia issues Learn more about our work across the world Solving the world's greatest problem—lostness Learn how you can get involved in the Great Pursuit an International Mission Board missionary emeritus who shared the gospel among East Asian Affinity Peoples in Japan where Maddox grew up on Waldkirch Avenue surrounded by neighbors who soon became friends She graduated from West End High School there in 1948 Dorothy left Nashville to attend Carson-Newman College (now University) Her time in college was cut short when her mother died unexpectedly and she went home to care for her father and to work They married in 1956 and began their life’s work in Christian ministry Dorothy supported Wayne in his work at First Baptist Church in Fort Walton Beach Dorothy and Wayne felt God’s call to move to the island of Okinawa The Foreign Mission Board (now International Mission Board) appointed them missionaries in 1969 Dorothy said that God put her favorite places—the mountains of Tennessee and the beaches of Florida—together and called it “Okinawa.”   The Maddoxes arrived in Okinawa in 1970 and stayed in Japan until they retired in 1990 where they continued their life in ministry Dorothy and Wayne began assisting those in need through the Samaritan Hands Ministry Dorothy also volunteered as a counselor with Habitat for Humanity In 2013 she moved to Tennessee to be close to her daughters and grandchildren There she became a member of First Baptist of Jefferson City Maddox was preceded in death by her husband of 45 years Millie Maddox Ellis (Bill) and Barianne Maddox Wilson six grandchildren and five great-grandchildren Read an obituary here SBC | The Lottie Moon Christmas Offering® is a registered trademark of Woman's Missionary Union You are using an outdated browser. Please upgrade your browser or activate Google Chrome Frame to improve your experience Power provider Stadtwerke Waldkirch has built a 264 kW PV system in Germany with Sunman’s glass-free modules as a titanium rooftop at the project site made it impossible to use conventional modules Germany's Stadtwerke Waldkirch commissioned a PV system with a nominal output of 245 kw in February on the roof of one of its buildings Chinese manufacturer Sunman supplied glass-free crystalline modules for the installation and now wants to bring these products to the broader European market Thanks to the use of glass fibre-reinforced plastic (GRP) the panels only placed an additional load of 4 kilograms per square meter on the roof Stadtwerke Waldkirch said that it has been planning to install a PV system on the rooftop since 2011 the titanium roof could not accommodate conventional solar modules as they would place an additional load on it of at least 15 kilograms per square meter its PV panels are particularly lightweight because of patented composite materials it uses to replace the glass panels on the front side The modules are only about 2 millimeters thick and can be brought to the roof with a total load of 3.5 to 5 kilograms per square meter The modules are thinner and lighter than common glass models but offer the same durability and performance as standard modules with crystalline silicon cells In addition to the protective layers on the front and back sides the panels are laminated on both sides of the solar cells with multiple layers of waterproof polymer composites They offer a conversion efficiency of around 18% nonresidential rooftops throughout Europe with surface areas of ​​about 360 million square meters The company estimates that around 60% of these industrial rooftops cannot reliably support conventional solar modules that weigh between 15 and 20 kilograms per square meter “All industrial and commercial enterprises can provide their roofs with solar modules and cover a large part of their electricity needs with solar energy itself,” said Zhengrong Shi A solar pioneer, he was the founder of Suntech, which became the largest module manufacturer in the world in 2012. But the company subsequently ran into financial difficulties and was acquired by Shunfeng International Clean Energy in 2014 Shi was CEO of the company until August 2012 Sunman has already introduced its glass-free modules in Asia More articles from Petra Hannen 150watt solar panel that produces 20v but can not charge battery what do I do to this challenge issues will arise for recycling of such modules with composite material (same issues we are facing when it comes to unrecyclable plastics) Manufacturers should think of the complete lifecycle of their products may be more useful in covered cultivation but what is the cost Please be mindful of our community standards and website in this browser for the next time I comment Δdocument.getElementById( "ak_js_1" ).setAttribute( "value" By submitting this form you agree to pv magazine using your data for the purposes of publishing your comment Your personal data will only be disclosed or otherwise transmitted to third parties for the purposes of spam filtering or if this is necessary for technical maintenance of the website Any other transfer to third parties will not take place unless this is justified on the basis of applicable data protection regulations or if pv magazine is legally obliged to do so You may revoke this consent at any time with effect for the future in which case your personal data will be deleted immediately your data will be deleted if pv magazine has processed your request or the purpose of data storage is fulfilled Further information on data privacy can be found in our Data Protection Policy Δdocument.getElementById( "ak_js_2" ).setAttribute( "value" This website uses cookies to anonymously count visitor numbers. View our privacy policy. × The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this Close Die Publikation der Informationen von dieser Website ist ohne vorherige Zustimmung von Lesprom strengst verboten Softwarelizenzvertrag und Datenschutzrichtlinie Looking to access paid articles across multiple policy topics Interested in policy insights for EU professional organisations Extended reality has the potential to revolutionise the visualisation and planning of surgeries medical experts already working with such tools told Euractiv in an interview This article is part of our special report Metaverse sees first applications to reimagine reality Underwritten Produced with financial support from an organization or individual yet not approved by the underwriter before or after publication This article is part of our special report Metaverse sees first applications to reimagine reality The Spanish presidency of the EU Council wants an opt-out option for the primary use of health data in emergency situations and two mandatory ‘harmonised components’ for health record systems (EHRs) in the EU's health data regulation according to a position paper obtained by Euractiv We want to hear what you think about this article. Submit a letter to the editor or write to letters@theatlantic.com A collection of winning and honored images from this year’s nature-photo competition A collection of amazing recent images made with the Hubble Space Telescope Mourners of Pope Francis gathered at the Vatican scenes from the the second weekend of Coachella 2025 and landscapes of the Earth’s arctic and subarctic regions Mermod Freres-Ste Croix Suisse interchangeable cylinder music box Limonaire Freres Waldkirch fairground organ Circa 1876 Renaissance Revival Centennial center table with inlay of George Washington’s head flanked with dates 1776 and 1876 More than 450 registered bidders from 26 states and two countries packed the Sharonville Convention Center for the recent George E Several auction records in mechanical music were broken by auctioneers Dave and Frank Forsythe of Forsythe Auctions a native Cincinnatian who passed away in July 2003 had amassed a premier collection of mechanical music and other antiques that was known around the world Top lot of the day was a Bremond interchangeable cylinder orchestral music box with 12 19-inch cylinders; it sold for a record price of $82,500 Other mechanical music included a Mermod Freres Interchangeable Cylinder music box with three 241/2-inch cylinders that was hammered down for $49,500; a Symphonion Eroica hall clock went for $46,750; a Symphonion three-disc upright music box was $38,500; a Troll & Baker large interchangeable cylinder box with six 16-inch cylinders also hit $38,500 A Vaucher fils Paillard Revolver cylinder music box was $27,500; a John Manger large cylinder music box hit $33,000; a singing bird cylinder box $30,250; and a Sublime-Harmony-Piccolo-Bells interchangeable cylinder music box sold for $22,000 Other cylinder boxes included rare Nicole Freres Grand Overture cylinder music box $20,300; Orchestral cylinder box with 14-inch cylinder $10,450; Troll & Baker interchangeable box with table $6,600; a large 25-inch cylinder box marked BAB $20,900; and a Bremond Mandoline Organocleide box Other disc boxes included Symphonion style 25C $9,900; Imperial Symphonion 15-inch disc box $4,950; Regina Style 50 with serpentine case $7,150; Stella Concert 151/2-inch box $7,700; Regina 151/2-inch with carved case A Limonaire fairground organ found a new home at $24,750; an Arthur Bursens band organ fetched $16,500 and a large Arburo dance organ reached $17,600 Other interesting rdf_Descriptions included a small Symphonion disc box with lithograph animation that went for $7,150 $7,700; hand crank automaton box with monkey $2,200; folding table-top stereographoscope $7,700 and $6,050; Grand Roller organ with 24 rollers $7,150; Wurlitzer 103 Military Band Organ which included a rare Zon-O-Phone that sold for $8,250; Columbia Graphaphone BS $770; Talk-A-Phone disc phonograph with carving Some of the highlights of the clocks was a French conical pendulum clock for $4,400; mechanical bird clock $3,575; Gustave Becker wall clock with music box $2,310; and a mahogany English grandfather clock Theders also loved toys and a few examples included a 48-inch horse-drawn fire ladder wagon at $10,450 and Fleischmann ocean liners at $2,420 and $1,705 Prices include a ten percent buyer’s premium SunMan is introducing an innovative photovoltaic solution for lightweight roofs in Europe: a glass free solar module of less than 2mm thickness and two-thirds less weight with a yield comparable to glass-based solar modules Industrial buildings have plenty of roof space for photovoltaic systems but are frequently not structurally designed to bear the heavy weight of conventional glass solar modules SunMan from China has developed an innovative photovoltaic module without glass branded as “eArche” eArche modules are much thinner and lighter than conventional glass solar modules while at the same time offer the same weather resistance and performance as conventional modules with crystalline silicone cells The initial projects in Waldkirch in south Baden Switzerland and Spain have delivered good results “Thanks to a patented combination of materials our new style glass free solar modules function well and are easy to bond to the roof,” explains  Zhengrong Shi all industry and commercial rooftops can be covered with solar modules and supply a large part of the electricity consumption through solar energy.” The company will be showcasing this new technology at the Intersolar in Munich from 15 to 17 May 2019 Visitors will find the stand in Hall A1 (booth 612) Industrial companies usually have high energy requirements and correspondingly high electricity costs they could cover a large proportion of these needs themselves through photovoltaics (PV) on the roofs of their factory halls and other buildings According to a study by the Fraunhofer Institute for Wind Energy and Energy System Technology the overall flat roof surface area for non-residential buildings in the European Union totals 360 million square metres (2012) we assume that around 60 per cent of these industrial roofs cannot permanently support conventional solar modules weighing between 15 and 20 kilograms per square metre If the roof structure only permits minor additional loads there have hardly been any opportunities until now for exploiting the PV potential,” says Dr Zhengrong Shi How much weight can be loaded on roofs also depends on the local wind and snow conditions the greater the chance of installing PV modules over the entire roof area,” Shi continues SunMan has developed an innovative technology to make ultra-light PV modules that with a total weight of 3.5 to 5 kilograms per square meter (kg/m²) This makes the PV modules 10 to 15 kg/m² lighter than conventional solar modules The light eArche modules offer numerous benefits to installers The low weight and thin structure make it easy and inexpensive to transport and store large quantities of the modules These features also ensure fast installation and in most cases enable the modules to be attached to the roof using simple bonding technology It is not necessary to install a heavy mounting system and no penetration is required This protects the roof from being damaged and ensures that the roof remains waterproof once the solar power system has been installed The low weight of the PV modules is due to patented composite materials that replace the glass pane on the front side of conventional solar modules we can now also produce green power on the roof of the town hall in Waldkirch and further expand our renewable generation portfolio,” says Thorsten Ruprecht “It wasn’t possible to install heavier solar modules with glass panels on the titanium zinc roof We’re now in a position to implement our planned projects that were previously thwarted by the structural loading problems.” The ultra-light PV modules with an installed capacity of 190 kilowatts A further 55 kilowatts will soon be added on the foyer roof “We’re now able to provide between 80 and 100 households with solar power,” Ruprecht continues 34 kilowatt eArche modules were installed on the trapezoidal sheet roof of the water treatment plant in Küsnacht So far a yield of 1,065 kilowatt hours per installed kilowatt peak has been achieved the eArche panels are just as robust and durable as conventional solar modules This is due to the weatherproof properties of the composite materials being used in both cases the durability of the module is not determined by glass but rather by the polymer materials (EVA and fluorine layers coated PET) laminated on the backside of the module there are multiple layers of waterproof polymer composite materials being laminated on both sides of crystalline silicon solar cells apart from the protection layers on the front and back surfaces of the solar module This structure further enhances the protection of the solar cells from the hazardous environment comparing to the conventional modules As crystalline silicon solar cells are used in the module eArche modules have similar electrical performance to that of conventional modules around 18% much higher than the efficiency of thin-film solar modules Following the successful market entry in Asia SunMan is now introducing the modules throughout Europe “The power generated is intended mainly for self-consumption,” Shi says industrial and commercial companies have a decisive advantage: their energy requirements are highest during the sunny hours of the day when the solar power system generates the most electricity By consuming the solar power generated themselves industrial companies therefore save on a large proportion of the more expensive industrial and commercial power but are also an economically astute investment The more solar power is used by the companies themselves “In future it will also be possible to put these advantages to practical use for companies with lightweight roofs,” Shi explains Stay informed, get our free newsletter twice a week. Register here Join our pv Guided Tours at The smarter E Europe 2019 (Intersolar Europe, ees Europe, Power2Drive, EM-Power), register here Looking to stay on top of all relevant industry and business news? Click here to subscribe to our free twice-weekly pv Europe newsletter. A podcast for investors on the opportunities and risks of the solar market The pv Europe editorial team offers their own analysis and discusses current topics with experts Experts say the provincial government’s proposed legislation aimed at restricting short-term rentals is a welcome first step — but it doesn’t go much further than that says the increased restrictions on short-term rentals but it’s only a smidgen of hope in the greater picture of the province’s housing crisis “The amount of homes that can be returned to the market by cracking down on Airbnb is only a drop in the bucket of Vancouver’s and other cities’ housing needs,” Waldkirch said it’s really only a tiny amount of what we need.” On Monday, the B.C. government proposed a crack down on short-term rentals by tabling legislation to create more “long-term homes.” The legislation will include increasing fines and strengthening tools for local governments; returning more short-term rentals into long-term homes for people; and establishing provincial rules and enforcement the provincial government says 40 to 50 per cent of short-term rental listings are non-compliant with its new legislation “People will no longer be able to buy multiple properties and use them for STR platforms to make huge profits while taking away homes for people who desperately need them,” Housing Minister Ravi Kahlon said But Waldkirch says the legislation is not nearly enough to make a difference when considering the number of homes Vancouver and B.C He refers to ending Vancouver’s “apartment ban,” or single-family zoning rules as an action that could have a more measurable impact in alleviating some of the pressure on the city’s housing stock Waldkirch says the strengthening of enforcement and information gathering tools for local governments across the province is the “most welcome part” of the legislation and he’s excited to see smaller communities benefit from this influx of data director of the city program at Simon Fraser University says he’s also excited for the information and data on short-term rentals that’s expected to come out of this new legislation because he thinks it will help ensure accountability and transparency is a part of this proposed legislation Yan says it’s important that this new legislation didn’t involve an outright ban on short-term rentals He says its targeting of properties bought specifically for short-term rentals rather than a laneway home or basement unit that’s part of a permanent residence “Short term rental does serve a function especially in smaller communities that might not have the tourist infrastructure,” Yan said Waldkirch adds that there’s also a large demand for short-term rentals in Vancouver because of its “desperate shortage of hotel space” which harms the city’s economy Waldkirch says he thinks British Columbians should manage their expectations of this proposed legislation if it’s rolled out “This isn’t something that’s going to make a major difference to the housing crisis,” he said The bill will move to a second reading in the legislature on Tuesday -With files from Charlie Carey and Liza Yuzda The federal council and the NDP caucus decided on the local MP Jendhel May Sico is described as a person who lived her life to the fullest she was celebrating the Lapu-Lapu Day festival with those she loved most -- her cousin President Donald Trump’s latest trade threat has the Canadian film industry bracing for impact but nobody is quite sure what that impact would be Killed in a car-ramming attack on Saturday she was celebrating the Lapu-Lapu Day festival with those she loved most -- her cousin speaks to reporter Kier Junos about the day their lives changed forever Huge crowds lined the streets of Vancouver to cheer on 25,000 runners making their way around the city for the 53rd annual BMO Marathon on Sunday The accused person in the deadly car attack at the Lapu-Lapu Day block party in Vancouver on April 26th appeared at the provincial Court of British Columbia 30-year-old Kai Ji Adam Lo appeared via video in court Four people remain in critical condition in hospital and another two remain in serious condition five days after the deadly attack at the Lapu-Lapu Day festival in Vancouver on April 26 Jack Rabb has more on the efforts to support the victims Juror illness led to an early adjournment Thursday with NHL player Taylor Raddysh expected to continue his witness testimony Friday Michelle Mackey has the latest in the sex assault trial of five ex-world junior hockey players Listen to NewsRadio Vancouver live anytime and get up-to-the-minute breaking-news alerts weather and video from CityNews Vancouver anywhere you are – across all Android and iOS devices has approved prefabricated housing designs so developers don’t have to wait for a lengthy approval process before they can start building but some housing experts say this isn’t enough The provincial government says the designs apply to small-scale multi-unit housing types and are free for the public to use building codes and are customizable for different lot sizes and configurations “Most of the designs are based on ‘building blocks’ that can be mixed and matched to add features such as a garage or bedrooms and stacked up to three storeys high,” the government said in a news release Thursday a design and build company that creates custom homes He says developers still face a lot of hurdles when creating pre-approved designs like the fact that each municipality has different zoning rules and regulations “I’ve been building laneway houses for 15 years and every single city that you go to has different rules,” he said “Some of these municipalities pile on these development permits that require you to jump through all sorts of hoops.” a standard design in Vancouver might have to be modified in Burnaby and not allowed at all in North Vancouver Peter Waldkirch with Abundant Housing Vancouver says this latest move is a good step by the province but he says the province needs to consider all residential building types “We need standardized designs and zoning for apartment buildings so that we can build the homes we need,” Waldkirch said housing demand was more than just multiplexes,” he said government says there are also designs for accessory dwelling units Home   News   Article SAM'S latest sofa gives the people of Wick a place to take a seat and rest their weary feet This new arrival at Sams Furniture is made from a solid piece of wood and has been donated to the town as a thank-you for "continuous support" It was put in place outside the High Street shop last week and already has been the subject of much admiration with around 1000 likes on the store's Facebook page More than 150 social media comments have been made about this new feature which is proving to be a popular focal point and has been widely photographed as everyone tries it out Business owner Sam Salim explained: "Local councillors were asking retailers to find quirky ways to make the town more appealing "The sofa has gained a tremendous response on Facebook – it is kind of overwhelming The town has been good to me and this is a thank-you." He added: "I have seen tourists taking a seat on it and having their pictures taken While enjoying a takeaway coffee on the new seat on Monday morning although it wasn't quite as comfortable as a pocket-sprung sofa Der Prozess gegen TikTok-Star Maurii Pastore wird an einem anderen Tag fortgesetzt Freiburgs Polizeipräsident sieht sich in einem Video von Pastore verunglimpft Vor dem Amtsgericht Waldkirch (Kreis Emmendingen) hat am Mittwoch der Prozess gegen den TikToker Maurii Pastore begonnen soll in einem seiner Sketche auf der Videoplatform "TikTok" den Freiburger Polizeipräsidenten und dessen Assistentin verunglimpft haben Darin ging es unter anderem um das Thema Karriere gegen Sex Der Prozess gegen Maurii Pastore ist am Mittwoch circa eine Stunde nach seinem Beginn auf unbestimmte Zeit vertagt worden Vorher war der Prozess zweimal unterbrochen worden weil der Verteidiger von Maurii Pastore einen Befangenheitsantrag gegen die Richterin gestellt hatte Der Verteidiger sah die Richterin als befangen weil diese in unüblichem Ausmaß Auskünfte über die Finanzen des Angeklagten eingeholt haben soll Laut der Richterin und der Staatsanwaltschaft ist das Vorgehen so üblich um den Tagessatz im Falle einer Verurteilung festlegen zu können Das Amtsgericht Waldkirch hatte zunächst einen Strafbefehl samt einer Geldstrafe von 4.800 Euro gegen den TikToker verhängt Maurii Pastore hat dagegen Einspruch eingelegt Sein Verteidiger argumentiert Medienberichten zufolge damit dass das Video satirisch gemeint und damit von der Kunstfreiheit geschützt sei Das Video von Maurii Pastore findet sich auf seinen Kanälen unter anderem auf Youtube und TikTok und wurde mittlerweile fast 1,5 Millionen mal aufgerufen So berichtete SWR Aktuell im Fernsehen im Januar 2023 über das Leben von TikToker Maurii Pastore Vor seiner Karriere als sogenannter "Creator" auf dem Videoportal TikTok arbeitete Pastore bei der Autobahnpolizei in Weil am Rhein (Kreis Lörrach) In einer Mischung aus Saarländisch und Alemannisch schlüpft der Elztäler mit italienischen Eltern in verschiedene Rollen - die "Muddi" Mit einfachen Hilfsmitteln wie Perücken und seiner Handykamera dreht er die Kurzvideos die sich vor allem junge Menschen unter 25 Jahren anschauen Nach kurzer Zeit gingen seine Videos viral und er hängte seinen Polizistenjob für TikTok an den Nagel VancouverNewsVancouver council approves mayor’s proposed freeze on new supportive housingBy Regan Hasegawa and Andrew WeichelPublished: February 26, 2025 at 9:10AM EST Twitter feed ©2025 BellMedia All Rights Reserved Unter Umst�nden sammelt BotTalk personenbezogene Daten f�r eigene Zwecke und verarbeitet diese in einem Land mit nach EU-Standards nicht ausreichenden Datenschutzniveau Durch Klick auf "Akzeptieren" geben Sie Ihre Einwilligung f�r die Daten�bermittlung die Sie jederzeit �ber Cookie-Einstellungen widerrufen k�nnen Wenn Sie auf diesen Artikel von badische-zeitung.de verlinken m�chten k�nnen Sie einfach und kostenlos folgenden HTML-Code in Ihre Internetseite einbinden: Lost Gravity in Walibi Holland – Der Big Dipper Prototyp Fotos © Eric Christopher Staube (Screenshots) via ZDFmediathek und CoasterTopia via CC4.0 Cultural Studies (BA Scenic Arts) in theatre and film direction media and pop culture as well as staging dramaturgy and processes Copyright © 2014. Created by Meks. Powered by WordPress.