Cepsa will supply 100% renewable electricity to these facilities
IONITY has launched its first high-power charging station in Spain
the new site is equipped with 4 high power charging points each with a capacity of 350 kW
These chargers are compatible with the European standard Combined Charging System (CCS) and reduce charging times for current and future EVs significantly
Offering 1,2 Mega Watt of power for the 4 high power chargers
the IONITY station is among the fastest in Spain
IONITY has prepared the station for 2 additional high-power chargers that can be added depending on the demand
Cepsa will supply 100% renewable energy to these facilities
This first station in Spain is the latest chapter in the expansion of IONITY’s pan-European charging network
The objective is to make long distance EV travel a reality
Pallejà is also one of 340 ultra-fast charging stations being deployed within the Europe-E project
covering main routes across 13 EU Member States and along all 9 TEN-T Core Network Corridors and the Core Network
The EUROP-E project is co-financed by the Connecting Europe Facility of the European Union
In July 2018, Cepsa signed a collaboration agreement with IONITY
the European high-performance charging network formed by the BMW Group
for the installation of electric charging points at its service stations in Spain and Portugal
Cepsa currently has an extensive network of close to 1,800 service stations in Spain and Portugal
The Cepsa management team is focused on offering a clear model of customer orientation
Cepsa sells top quality fuels at its Service Stations
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gut microorganisms harbour and exchange antibiotics resistance genes
Using shotgun sequencing-based metagenomics
we analysed the partial eradication and subsequent regrowth of the gut microbiota in 12 healthy men over a 6-month period following a 4-day intervention with a cocktail of 3 last-resort antibiotics: meropenem
Initial changes included blooms of enterobacteria and other pathobionts
such as Enterococcus faecalis and Fusobacterium nucleatum
and the depletion of Bifidobacterium species and butyrate producers
The gut microbiota of the subjects recovered to near-baseline composition within 1.5 months
which were present in all subjects before the treatment
remained undetectable in most of the subjects after 180 days
Species that harbour β-lactam resistance genes were positively selected for during and after the intervention
Harbouring glycopeptide or aminoglycoside resistance genes increased the odds of de novo colonization
the former also decreased the odds of survival
Compositional changes under antibiotic intervention in vivo matched results from in vitro susceptibility tests
Despite a mild yet long-lasting imprint following antibiotics exposure
the gut microbiota of healthy young adults are resilient to a short-term broad-spectrum antibiotics intervention and their antibiotics resistance gene carriage modulates their recovery processes
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The high-quality reads have been deposited in the European Nucleotide Archive with accession number ERP022986. Relative abundances of taxa and functional features can be downloaded at http://arumugamlab.sund.ku.dk/SuppData/Palleja_et_al_2018_ABX/
The human intestinal microbiome in health and disease
Comparative metagenomic study of alterations to the intestinal microbiota and risk of nosocomial Clostridum difficile-associated disease
An obesity-associated gut microbiome with increased capacity for energy harvest
Disentangling type 2 diabetes and metformin treatment signatures in the human gut microbiota
Gut metagenome in European women with normal
A metagenome-wide association study of gut microbiota in type 2 diabetes
Inflammation drives dysbiosis and bacterial invasion in murine models of ileal Crohn’s disease
Microbiota modulate behavioral and physiological abnormalities associated with neurodevelopmental disorders
Consensus report: faecal microbiota transfer—clinical applications and procedures
Towards a more comprehensive concept for prebiotics
Effects of probiotics on gut microbiota: mechanisms of intestinal immunomodulation and neuromodulation
glucose metabolism and body weight regulation: a review of the literature
Metabolic and metagenomic outcomes from early-life pulsed antibiotic treatment
The human microbiome: at the interface of health and disease
Cognitive impairment by antibiotic-induced gut dysbiosis: analysis of gut microbiota-brain communication
Intestinal microbiome is related to lifetime antibiotic use in Finnish pre-school children
Association of antibiotics in infancy with early childhood obesity
Antibiotic exposure in infancy and risk of being overweight in the first 24 months of life
Association between the use of antibiotics and new diagnoses of Crohn’s disease and ulcerative colitis
Infant antibiotic exposures and early-life body mass
The antibiotic resistome: the nexus of chemical and genetic diversity
Country-specific antibiotic use practices impact the human gut resistome
Determining the long-term effect of antibiotic administration on the human normal intestinal microbiota using culture and pyrosequencing methods
Same exposure but two radically different responses to antibiotics: resilience of the salivary microbiome versus long-term microbial shifts in feces
Effects of gut microbiota manipulation by antibiotics on host metabolism in obese humans: a randomized double-blind placebo-controlled trial
Microbial diversity in individuals and their household contacts following typical antibiotic courses
The initial state of the human gut microbiome determines its reshaping by antibiotics
Selective decontamination of the oropharynx and the digestive tract
and antimicrobial resistance: a 4 year ecological study in 38 intensive care units in the Netherlands
Identification and assembly of genomes and genetic elements in complex metagenomic samples without using reference genomes
Metagenomic species profiling using universal phylogenetic marker genes
function and diversity of the healthy human microbiome
Temporal and technical variability of human gut metagenomes
Updates on the sporulation process in Clostridium species
Extensive impact of non-antibiotic drugs on human gut bacteria
An integrated catalog of reference genes in the human gut microbiome
CARD 2017: expansion and model-centric curation of the comprehensive antibiotic resistance database
Improved annotation of antibiotic resistance determinants reveals microbial resistomes cluster by ecology
Carbapenemases: the versatile β-lactamases
Acquired vancomycin resistance in clinically relevant pathogens
a resistance-nodulation-cell division pump effluxing multiple antibiotics in Acinetobacter baumannii
Study of the correlation of imipenem resistance with efflux pumps AdeABC
AdeDE and AbeM in clinical isolates of Acinetobacter baumannii
knowledge and principle: back to metabolism in KEGG
Enhanced efflux activity facilitates drug tolerance in dormant bacterial cells
Gut inflammation provides a respiratory electron acceptor for Salmonella
Depletion of butyrate-producing Clostridia from the gut microbiota drives an aerobic luminal expansion of Salmonella
Precision editing of the gut microbiota ameliorates colitis
Integration and visualization of host-pathogen data related to infectious diseases
Bacterial quorum sensing: its role in virulence and possibilities for its control
Microbiota-mediated colonization resistance against intestinal pathogens
Fecal microbiota transplantation for Clostridium difficile infection: systematic review and meta-analysis
Gastrointestinal microflora studies in late-onset autism
Real-time PCR quantitation of Clostridia in feces of autistic children
Distinct microbiome-neuroimmune signatures correlate with functional abdominal pain in children with autism spectrum disorder
Intestinal microbial variation may predict early acute rejection after liver transplantation in rats
Richness of human gut microbiome correlates with metabolic markers
Systemic absorption of oral vancomycin in patients with Clostridium difficile infection
Absorption of oral aminoglycosides following bone marrow transplantation
MOCAT: a metagenomics assembly and gene prediction toolkit
A genomic update on clostridial phylogeny: Gram-negative spore formers and other misplaced clostridia
Fast and accurate short read alignment with Burrows-Wheeler transform
Search and clustering orders of magnitude faster than BLAST
Proportionality: a valid alternative to correlation for relative data
Normalization and microbial differential abundance strategies depend upon data characteristics
The statistical analysis of compositional data
Roux-en-Y gastric bypass surgery of morbidly obese patients induces swift and persistent changes of the individual gut microbiota
Download references
This work was funded by an international alliance grant from The Novo Nordisk Foundation Center for Basic Metabolic Research
which is an independent Research Center at the University of Copenhagen partially funded by an unrestricted donation from the Novo Nordisk Foundation (grant no
Our work was also funded by the TARGET research initiative (Danish Strategic Research Council [0603–00484B])
the Danish Diabetes Academy supported by the Novo Nordisk Foundation
the Danish Council for Independent Research (Medical Sciences)
was funded by FP7 METACARDIS HEALTH-F4-2012-305312
These authors contributed equally: Albert Palleja
Novo Nordisk Foundation Center for Basic Metabolic Research
Charité-Universitätsmedizin Berlin and Max Delbruck Center for Molecular Medicine
Max Delbruck Center for Molecular Medicine in the Helmholtz Association
Freie Universität Berlin Humboldt-Universität zu Berlin and Berlin Institute of Health
University of Heidelberg and European Molecular Biology Laboratory
Beijing Advanced Innovation Center for Food Nutrition and Human Health
College of Food Science and Nutritional Engineering
State Key Laboratory of Quality Research in Chinese Medicine/Macau Institute for Applied Research in Medicine and Health
Macau University of Science and Technology
participated in the protocol design and application and in the participant recruitment and selection
performed sample collections and carried out patient phenotyping
performed shotgun metagenomics sequencing and taxonomic profiling
All authors contributed to data interpretation
All authors read and approved the final manuscript
The authors declare no competing interests
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations
legends for Supplementary Tables and Supplementary Datasets
Comparison between taxa and KEGG function abundances at baseline (D0) and at subsequent time points (D8
D180) using a two-sided Wilcoxon signed-rank test
Sample information and read quality control statistics
Predicted and manually curated gene assignments (taken from GenBank) for 3 well-characterized species such as Salmonella typhimurium
Species that differentially changed their abundance (two-sided Wilcoxon signed-rank test) following antibiotic treatment (from Supplementary Table 1) contrasted with their extent of relative growth inhibition from Maier et al.38
Extent of enrichment (significantly higher number of genes) of ARGs for the drugs used in this study (and multidrug efflux pumps) in species enriched under intervention versus not (from Supplementary Table 1)
Gene-level differentially abundant ARGs under intervention
relative to their significantly differential prevalence in genomes in enriched versus depleted species among those differentially abundant under intervention (from Supplementary Table 1)
Associated data for Supplementary Figure 6
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DOI: https://doi.org/10.1038/s41564-018-0257-9
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Checkers and Rally's has opened a corporate unit in Orlando at 10793 South John Young Parkway
"The Orlando location stands as a testament to the brand's enduring ambition," Alvaro de Palleja
chief restaurant officer at Checkers Drive in Restaurants
"Checkers and Rally's model has proven itself in the QSR and drive-thru space and we are well-versed in the operations involved
We're eager to bring this new prototype to the Orlando market
providing more opportunities for job creation and business growth to the area."
The location features an updated prototype
marking the brand's first corporate store with the new look
"We view this opening as a reiteration of our unwavering commitment to growth and progress," Kris McDonald
VP of development at Checkers and Rally's Drive in Restaurants
"This expansion marks a pivotal step forward in our brand's strategic development plan
community engagement and culinary excellence
the new restaurant promises to elevate the taste preferences of Orlando residents and visitors alike
Checkers and Rally's brings aggressive plans to develop and grow our presence in Florida."
Checkers and Rally's merged in 1999 to form one concept
The brand is targeting markets in Wisconsin
Get the latest news and resources from QSR Web
often boosting the capacities of thousands of people
taking on the role of modern landmarks alongside stadiums
there are small-scale multi-purpose sports halls
or present in rural areas with limited access to other public amenities
these venues embrace and expand their multi-purpose role by offering opportunities to play
Arena do Morro - Herzog & de Meuron. Image © Iwan BaanIn many rural and suburban areas, these halls are the largest interior spaces publicly available. From sports competitions and physical education classes to community fairs and town meetings, these spaces show versatility, accommodating a wide range of activities on a daily basis. With a relatively simple architectural image, they fulfill a complex and noble social function as an active and representative public building.
Arena do Morro - Herzog & de Meuron. Image © Iwan BaanDesigning such venues involves negotiating between limited resources
and the need to create a representative image
The same duality is reflected in the urban aspect of such halls: while their volume and dimensions stand in contrast to the surrounding urban fabric
Their representational role requires careful urban integration
ensuring accessibility for the entire community
Related Article Architecture Beyond Humanity: Designing for Non-Human Species
Kura Kura Badminton Courts - IBUKU + Studio Jencquel. Image © Tommaso RivaTo further strengthen the community's connection to the space, local resources, and vernacular craftsmanship can be integrated into the design of these facilities, preserving the area's cultural heritage. Through all of these combined elements, sports halls hold the ability to reflect and solidify a community's identity and sense of place, anchoring a wide variety of social activities.
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