A new conservation initiative has taken root in the Judean Hills, where Keren Kayemeth LeIsrael – Jewish National Fund (KKL-JNF) has partnered with the Butterfly Enthusiasts Israeli Association to protect one of Israel’s rarest butterflies The project centers on planting 60 seedlings of the large-fruited milk-vetch plant crucial for the survival of the protected Tomares Nesimachus butterfly among Israel’s 14 protected butterfly species faces a precarious future due to its complete dependence on the large-fruited milk-vetch plant as a place to lay its eggs While the plant typically thrives in northern Israel The initiative sparked when members of the Butterfly Enthusiasts Israeli Association discovered the butterfly on a milk-vetch plant in KKL-JNF’s Begin Park which covers approximately 2,000 acres in the heart of the Jerusalem Mountains This finding catalyzed a coordinated conservation effort: KKL-JNF collected seeds from existing plants and transferred them to its Eshtaol Nursery in the Judean foothills where 60 seedlings were successfully cultivated These young plants have now been reintroduced to the Begin Forest through a collaborative planting effort involving community volunteers “This initiative is a wonderful example of collaboration between communities and professional organizations,” says Nurit Hibsher head of the Forest Department at the KKL-JNF Central Region “Preserving biodiversity and protecting rare butterflies like the Tomares Nesimachus is an integral part of our vision for safeguarding nature and the environment in the Judean Hills.” head of the KKL-JNF Seed and Nursery Department said the organization “leads the way in rare plant species propagation and reintroduction Our comprehensive approach encompasses seed collection and field maintenance strategies to ensure successful reintegration of these precious species into our forest ecosystems.” By preserving both the rare butterfly species and its essential host plant this initiative demonstrates the vital connection between plant and animal conservation in maintaining healthy ecosystems Presentation of the L Festival Flamenco from Tomares at the IAF El Festival Flamenco City of Tomares, which is one of the oldest, most prestigious and long-standing in Andalusia, will celebrate its 50th anniversary in style, on Saturday, July 19 at 22 p.m., in Los Jardines del Conde del Tomares City Council with a luxury line-up: with two of the greatest young national singers and the director of the Andalusian Institute of Flamenco (IAF) Festival that this year celebrates a very special date half a century being a reference in the dissemination of deep art and beyond our borders through which almost all the greats of the flamenco -Camarón most prestigious and traditional in Andalusia An event that also included the president of the Peña Flamenco Culture of Tomares the president of the Provincial Federation of Seville of Flamenco Entities the delegate for Major Festivals of the Tomares City Council and some of the great figures who will be protagonists such as the singer María Terremoto the dancer Manuela Carrasco and the guitarist The Pearl "today is a reason for joy because we are presenting the lineup for the 50th edition of the Festival Flamenco Tomares in a privileged environment such as the one in which this beautiful building is located headquarters of the Andalusian Institute of FlamencoCongratulations to the director of the Andalusian Institute of Flamenco for how much and how well he is working for the flamenco in Andalusia I want to highlight the importance that Culture and the Flamenco something very much ours and we must give it the importance it deserves "It is not easy for a peña turns fifty years old nor does it have fifty editions of its festival flamenco"Every year we improve which has become a benchmark in the province of Seville “A great poster that I also want to thank the Peña Cultural Flamenca de Tomares but this great poster is the result of the work of the Peña It is not easy for a peña turns 50 years old nor does it have 50 editions of its Festival FlamencoEvery year we improve and we are on our way to becoming a benchmark Festival in Andalusia and that is thanks to everyone's work and yours I want to thank all the presidents who have passed through the peñaTimes have changed a lot a group of friends who love flamenco How did you manage to carry out this great project such as the Peña Flamenco Cultural of Tomares" the director of the Andalusian Institute of Flamenco has specified that "the IAF has been chosen for the presentation of the L Festival Flamenco defend and protect the flamencoCongratulations to the mayor of Tomares for the excellent lineup and luxurious programming you've designed for the 50th edition of this festival A lineup you've designed with the support of the local community with the Peña Cultural Flamenca de Tomares with the direct work you do with the others peñaYes because from the Tomares City Council I attest to the work you do for culture and flamenco because you care about generating new audiences and the emergence of new talents This work you do throughout the year and culminates in this Festival according to the critic of flamenco Antonio Ortega “with two artists who are at the top of the young ladder: María Terremoto and Israel Fernández they were talked about as two diamonds in the rough but now they are two young figures of the flamenco who are at the top And it's a great blessing that they'll be sharing the bill with someone for whom she is a great role model: Manuela Carrasco This means that those of us who will be taking to the stage on July 19th will not only bring our professionalism but also all the love and affection; it's going to be a very special day I don't want to forget to mention the amazing lineup that's been prepared for the finale The closing party of the L Festival Flamenco Tomares will be played by El Perla on guitar Tickets (25 euros) are on sale online at www.giglon.com and in person at the Peña Flamenco Cultural of Tomares (C/ José Monje Cruz Camarón de la Isla email and website in this browser for future purposes Privacy Policy Agreement * I agree to the terms and conditions of the Privacy Policy. The Voice of the Flamenco in the World.  Please enter your username or email address to reset your password Students in the McCourt School’s client-based capstone course have analyzed hundreds of decades-old case files to advance research on exoneration The Making an Exoneree program has amassed a database of more than 300 cases for consideration over the past six years PJI partnered with graduate students in the McCourt School’s client-based capstone course to analyze the case files and provide guidance on data collection and organization for the future of the Making an Exoneree program PJI previously partnered with McCourt School capstone students on program evaluation for its two re-entry programs: the Pivot Program and the MORCA-Georgetown Paralegal Program PJI receives hundreds of cases that are reviewed by a small group of staff and students Each case provides detailed information about the life and wrongful conviction of the potential exoneree “We learn so much that is upsetting and unknowable,” said Danny Tomares (MPP‘23) “There are counties and states where we don’t have any way of getting information and people whose stories will never be known.” The McCourt students conduct extensive research and data analysis to assess different factors that impact the likelihood of a wrongful conviction identifying commonalities or trends in PJI’s data and how it compares to national databases “Finding patterns and understanding how they break down by age the more patterns will tell you,” said Shira Davidson (MPP’23) “It is strange to be brushing up against something so relevant,” said Tomares who are so close to a case that is being discussed nationally is an incredible opportunity that grounds innocence work in the real people who are behind the text and numbers on a screen.” Building on the research conducted by the McCourt students PJI hopes to increase its own understanding and scholarship of “a field where further research and collaboration are so important to bringing more justice to people who have been wrongfully convicted,” said Davidson Metrics details Bacterial Ribonucleoprotein bodies (BR-bodies) play an essential role in organizing RNA degradation via phase separation in the cytoplasm of bacteria BR-bodies mediate multi-step mRNA decay through the concerted activity of the endoribonuclease RNase E coupled with the 3′-5′ exoribonuclease Polynucleotide Phosphorylase (PNPase) studies indicated that the loss of PNPase recruitment into BR-bodies led to a significant build-up of RNA decay intermediates in Caulobacter crescentus it remained unclear whether this is due to a lack of colocalized PNPase and RNase E within BR-bodies or whether PNPase’s activity is stimulated within the BR-body We reconstituted RNase E’s C-terminal domain with PNPase towards a minimal BR-body in vitro to distinguish these possibilities We found that PNPase’s catalytic activity is accelerated when colocalized within the RNase E biomolecular condensates partly due to scaffolding and mass action effects disruption of the RNase E-PNPase protein–protein interaction led to a loss of PNPase recruitment into the RNase E condensates and a loss of ribonuclease rate enhancement We also found that RNase E’s unique biomolecular condensate environment tuned PNPase’s substrate specificity for poly(A) over poly(U) reduces RNase E phase separation both in vitro and in vivo This regulatory feedback ensures that under limited phosphate resources PNPase activity is enhanced by recruitment into RNase E’s biomolecular condensates a small domain (SD) and an intrinsically disordered region organized as a set of charged blocks these in vivo experiments could not distinguish whether exoribonuclease recruitment to BR-bodies was responsible for the mRNA decay fragment build-up or whether exoribonucleases have enhanced catalytic activity within BR-bodies here we provide direct in vitro evidence to show PNPase’s localization within RNase E biomolecular condensates stimulates the catalytic rate of PNPase and regulates the RNA substrate specificity of PNPase We then uncover how levels of a key PNPase enzymatic reactant and nutrient regulate phase separation This logical wiring of PNPase biochemistry with RNase E phase separation provides a way to regulate RNA decay processes in fluctuating phosphate nutrients These protein constructs allow us to consider how RNase E’s phase separation properties impact PNPase activity We visualized each mixture via phase contrast and fluorescence microscopy imaging to determine if the purified RNase E CTD-YFP could recruit PNPase-mcherry as a client We then calculated the fluorescence intensity ratio in the concentrated versus the dilute phase for each assembly Differences in the degree of protein enrichment in the dense phase directly vary the fluorescence intensity the unique chemical environment or these assemblies may alter the refractive index or impact the quantum yield of fluorescent proteins the crowding of fluorescent proteins nearby may lead to quenching the fluorescence signal the PR reflects the combinations of these effects PNPase partitions into RNase E biomolecular condensates by interacting with a C-terminal binding site on RNase E-CTD We found that PNPase enrichment into RNase E condensates was mediated by a specific protein–protein interaction (a) Phase-contrast and fluorescence microscopy images of RNase E biomolecular condensates RNase E and RNase E-∆PNP-BS contained a C-terminal eYFP tag and were present at 20 µM and PNPase-ASM (active site mutant) contained a C-terminal mCherry tag and was at a concentration of 1 µM (b) Average partitioning ratios and standard deviations are presented for RNase E There is no statistically significant difference between any PRs (p > 0.05) (c) Average partitioning ratios and standard deviations are presented for PNPase PNPase is only significantly recruited into RNase E biomolecular condensates (p < 0.001) PNPase does not significantly partition into RNase E biomolecular condensates (p > 0.05) (d) Average droplet circularity and standard deviations See the methods section for the droplet circularity calculation formula Data represent the average and standard deviation of n > 300 droplets from three replicates of images PNPase requires the 10 C-terminal residues of RNase E for enrichment within RNase E condensates This suggests weak fluorescent protein interactions can contribute to RNase E condensate morphology changes (b) Phase-contrast and fluorescence microscopy images of RNase E biomolecular condensates mixed with PNPase-ASM-mCherry or the PNPase-V104A/E224A/F233A-mCherry variant (c) Average partitioning ratios and standard deviations are presented for PNPase and the PNPase-V104A/E224A/F233A-mCherry variant The PNPase-V104A/E224A/F233A-mCherry variant does not significantly partition into RNase E biomolecular condensates (p > 0.05) Data represent the average and standard deviation of three replicates These results indicate that the GWW peptide outcompetes the C-terminus of RNase E for binding with PNPase lowering the amount of PNPase associated with the RNase E condensates These results suggest the potential of short peptides functioning as inhibitors to disrupt PNPase client recruitment into RNase E condensates These results suggest that poly(A) has a poor capacity to recruit PNPase and that the protein–protein interaction with the C-terminus of RNase E is likely the main driver of PNPase recruitment into RNase E condensates RNase E biomolecular condensates stimulate the ribonuclease functions of PNPase Here we found that PNPase recruitment into the RNase E condensates stimulated PNPase’s degradation of poly(A) substrates we found the addition of the maltose-binding protein (MBP) tag to RNase E solubilized the RNase E condensates We found that MBP-RNase E did not stimulate PNPase ribonuclease functions to the same level suggesting a role of RNase phase-separated environment upon PNPase stimulation (a) Rate of ribonuclease activity towards 25 ng/µL poly(A) for 5 µM PNPase alone 5 µM PNPase and 20 µM RNase E-∆BS (RNase E lacking the 14 C-terminal amino acids to which PNPase binds) and 5 µM PNPase and 20 µM MBP-RNase E (Maltose Binding Protein-RNase E fusion which cannot phase separate) (b) Ribonuclease activity of PNPase-V104A/E224A/F233A-mCherry (PNPase triple mutant lacking the ability to bind RNase E) and PNPase-V104A/E224A/F233A-mCherry mixed with RNase E No significant rate increase was observed when RNase E was added (p > 0.05) (c) Phase contrast and fluorescence microscopy images of RNase E CTD-YFP versus RNase E MBP-CTD-YFP Fusion of maltose-binding protein (MBP) significantly reduced the phase separation properties of RNase E (d) Ribonuclease activity towards 25 ng/µL poly(U) for five µM PNPase alone No significant difference in rate was observed (p > 0.05) (e) Ribonuclease activity of 5 µM PNPase after incubation with 10 µM or 100 µM GWW peptide (f) RNase E droplet formation stimulates PNPase polymerase activity The rate of PNPase polymerization of ADP into poly(A) is faster in the presence of RNase E droplets than with PNPase alone PNPase activity is not enhanced when the recruitment of PNPase to RNase E droplets is diminished (RNase E CTD∆BS) or when RNase E cannot form droplets (RNase E MBP-CTD) Data represent the average and standard deviation of at least three trials We next assessed how the phase-separated environment of RNase E impacted PNPase function. The PNPase-RNase E co-assembly degraded poly(A) at a rate of 274 ± 60 µg min−1 (mg PNPase)−1. This corresponds to a 3.4-fold enhancement of the rate of poly(A) degradation over PNPase alone (p < 0.001) (Fig. 4a) This result indicates that a direct interaction between RNase E and PNPase is required for the 3.4-fold rate enhancement of poly-A degradation In summary, we have considered the impact of the disruption of the RNase E-PNPase interaction through both RNase E variants (Fig. 4a) and PNPase variants (Fig. 4b) Both variants lead to a loss of PNPase recruitment into the RNase E condensates and a failure to stimulate PNPase’s breakdown of poly (A) These results suggest that the specific protein–protein interaction of RNase E’s C-terminal residues enhances One consideration in these assays was the ratio of RNase E:PNPase the copy numbers of RNase E range from 10 to 20 µM We examined assays at a 20:5 ratio of RNase E: PNPase to bias PNPase to be in its condensate-bound form lower PNPase concentrations ensure that the initial reaction rates are sufficiently slow for enzymatic characterization to distinguish condensate bound and free forms of PNPase We anticipate varied stoichiometry of this complex will also lead to optimizing this rate enhancement to ensure each RNase E scaffold engages one RNA substrate and one PNPase client crescentus PNPase alone has no substrate preference for poly(A) or poly(U) This increased enrichment suggests that the substrate selectivity of PNPase in the presence of RNase E is impacted by the increased poly(A) enrichment relative to poly(U) This suggests that there may also be contributions in how poly(A) and poly(U) impact the viscosity of the RNase E-PNPase condensates we considered if PNPase’s polymerase activity could be stimulated by RNase E CTD variants that do not colocalize with the RNase E condensates We found that PNPase polymerized ADP at a rate of 110 ± 6 mmol min−1 mg PNPase−1 in the presence of condensates formed from RNase E-∆PNP BS which was not significantly different from the polymerase activity of PNPase alone (p = 0.66) PNPase polymerized ADP at a rate of 106 ± 12 mmol ADP min−1 mg PNPase−1 and is not significantly different from PNPase alone (p = 0.87) the phase-separated environment has a mild 1.5-fold impact on PNPase polymerase activity These data suggest that the observed RNase E biomolecular condensates in cells are sites of RNA degradation instead of sites of polymerase activity We next considered three mechanisms for how RNase E could stimulate PNPase phosphorylase activity The first is that RNase E’s C-terminal binding site allosterically activates PNPase RNase E brings PNPase and poly(A) nearby via scaffolding stimulating enhanced exoribonuclease activity a third model considers the unique chemical environment of biomolecular condensates that concentrates both poly(A) and PNPase This third model builds upon the scaffolding effect to include the impact of a higher concentration of PNPase and poly(A) in the RNase E biomolecular condensates thereby increasing the kinetics of PNPase through mass action To test allostery without phase separation and scaffolding, we incubated PNPase with the GWW peptide from RNase E for 30 min before measuring exonuclease activity against poly(A). There was no significant difference in PNPase activity when 10 or 100 µM of the peptide was added (p > 0.05) (Fig. 4e) indicating that the RNase E GWW peptide does not allosterically activate PNPase While this known direct interaction between the GWW peptide and PNPase does not stimulate PNPase function the full-length RNase E may make additional unknown contacts with PNPase that mediate allosteric regulation Analysis of these constructs suggests that scaffolding in the dilute phase increases PNPase activity twofold one caveat for using a MBP fusion is that the bulky MBP tag may exert other steric effects upon the RNase E-PNPase complex in addition to diminished phase separation High sodium phosphate or low magnesium chloride levels dissolve the RNase E biomolecular condensates Caulobacter crescentus lives in diverse environmental conditions with fluctuating nutrients such as magnesium and phosphate We found that low phosphate levels stimulate RNase E phase separation in vivo and in vitro whereas high levels of phosphate lead to the dissolution of the RNase E biomolecular condensates (a) We considered the logic of how PNPase’s biochemistry is connected to RNase E’s phase separation PNPase utilizes a magnesium ion cofactor to catalyze the phosphorolysis of a single nucleotide (AMP) by adding inorganic phosphate to release nucleoside diphosphates (ADP) (b) Phase-contrast images of RNase E biomolecular condensates in 0 mM or 10 mM sodium phosphate (c) Turbidity measurement at 340 nm of 20 µM RNase E in a titration of magnesium chloride or sodium phosphate (d) Phase-contrast images of RNase E biomolecular condensates mixed with 1 mM poly(A) 5-mer (e) Rate of ribonuclease activity towards 25 ng/µL poly(A) for 5 µM PNPase alone or 5 µM PNPase and 20 µM RNase E in 10 mM sodium phosphate that does not form phase-separated assemblies (f) Effect of increased phosphate on BR-bodies in vivo (g) The average number of foci when cells are grown in Higgs medium supplemented with 30 µM and 1 mM phosphate Error bars represent the standard deviation from three biological replicates We proposed that protein–protein interactions between RNase E’s positively and negatively charged blocks play a key role in RNase E’s homotypic phase separation Whereas RNase E’s positively charged block interaction with negatively charged RNA regulates its RNA-stimulated phase separation we speculate that the negatively charged phosphates interact with the positively charged blocks in a way that competes with and disrupts key multivalent interactions that drive RNase E’s phase separation Phosphate ions may also directly compete for binding to the same interaction motifs or phosphate ions may alter RNase E’s conformation to prevent multivalent interactions Delineation of these models will require future structural studies to identify the multivalent interaction that mediates RNase E phase separation While 6 mM sodium phosphate was sufficient to drive the dissolution of RNase E droplets the equivalent ionic strength of sodium chloride was not sufficient to dissolve RNase E droplets this effect is not generally applicable to all divalent anions since sodium sulfate's equivalent ionic strength was insufficient to dissolve RNase E droplets To further decouple the effect of phosphate from sodium we titrated the potassium salts of chloride potassium phosphate drove the dissolution of RNase E droplets while the equivalent ionic strength of potassium chloride or potassium sulfate did not Given the impact of high phosphate on the dissolution of RNase E biomolecular condensates, we also hypothesized that RNase E would no longer stimulate PNPase’s activity under high phosphate conditions. Indeed, in 10 mM sodium phosphate, we found that the activity of PNPase was unaltered by adding RNase E (Fig. 5e) This adds an additional line of evidence that RNase E’s phase separation properties are critical to its ability to enhance PNPase functions the sensitivity of RNase E’s phase separation and PNPase regulation capabilities suggest that cytosolic phosphate concentrations may impact BR-body formation in vivo This indicates that ADP products of PNPase nuclease activity do not directly regulate RNase E’s phase separation The ability to avoid biomolecular condensate dissolution at high levels of ADP may be critical to maintaining the enhanced RNA decay within these assemblies The addition of these short poly(A) oligos did not cause the dissolution of RNase E droplets These results indicate that the ribonuclease activity products of PNPase do not cause RNase E biomolecular condensates to dissolve The logical wiring of RNase E condensate phase separation with PNPase enzymatic functions Our studies found that RNase E phase separation is sensitive to a critical reactant High phosphate nutrients dissolve RNase E CTD condensates in vitro Low phosphate nutrients stimulate phase separation and PNPase activity when the concentration of this critical phosphate nutrient is low Thus the availability of phosphate nutrients provides a negative feedback loop upon the regulation of RNase E condensate formation and function Our previous work has shown that RNA substrate availability stimulates BR-body formation RNA substrates provide a positive feedback loop that provides on-demand highly active RNase E condensates Our current simplified system of RNase E’s CTD and PNPase has allowed us to examine the effects of RNase E upon PNPase our studies are limited by the simplicity of the RNase E-CTD-PNPase assemblies versus the full compositional of BR-bodies in vivo the full-length RNase E additionally includes the N-terminal endoribonuclease domains and a binding site for the DEAD-box RNA helicase RhlB This full-degradosome brings the capacity to the breakdown of structured RNA that require RNase E’s endoribonuclease activity followed by the exoribonuclease activity of PNPase we expect the degradation rates of structured RNAs by PNPase alone to be very low adding full-length RNase E and RhlB’s capacity to unwind structured RNA substrates would likely lead to significantly higher rate enhancements in the context of structured RNA substrates that require a two-step decay process we suspect that co-localization of RNase E and PNPase is critical to channel the RNA intermediates from RNase E to PNPase and minimize RNA intermediate half-life In the absence of full-length RNase E localization with PNPase RNA decay intermediates that RNase E has initially degraded are expected to have a longer half-life before encountering the downstream PNPase enzyme The addition of the remaining clients (RNase D and RhlB) will very likely alter some of the protein–protein interactions within the condensate and may introduce new interactions The full degradosome's altered multivalent interaction networks may have a different chemical environment than the CTD alone This full degradosome chemical environment may impact viscosity increase crowding due to fully populated degradosome access RNA substrates to the ribonuclease enzymes and could further refine substrate selectivity Future comparison of unoccupied RNase E biomolecular condensates to BR-bodies with partial client occupancy to full client occupancy will yield considerable insights into the systems biology of BR-bodies This may suggest that long contiguous stretches of adenosine display preferences over isolated adenosine bases amongst oligoribonucleotide substrates results suggest that BR-bodies can fine-tune the half-lives of RNAs in cells and that polyadenylation may shape the available transcriptome future studies will be needed to examine the interplay of polyadenylation and BR-body functions in vivo and in vitro The coupling of enzyme recruitment and enzyme regulation enabled the spatial regulation of PleC function Our studies suggest that systematically evaluating biomolecular condensate effects through selective scaffolding and allostery can reveal how biomolecular condensates fine-tune enzyme functions Changes in the media’s phosphate concentrations may also increase PNPase activity clearing RNAs from cells crescentus in high phosphate would result in more ribosomes competing with BR bodies phosphate nutrient availability regulates BR phase separation and access to RNA substrates PNPase activity may be too low to drive the decay of RNAs low phosphate conditions in the presence of nucleotide diphosphates may bias PNPase's function as a polymerase This presents significant evolutionary constraints on microorganisms that live in low phosphate nutrient conditions This critical phosphate-BR-body feedback loop ensures robust PNPase activity as Caulobacter crescentus endures fluctuating availability of phosphate nutrients in the environment The mechanism of how phosphates dissolve RNase E condensates will require understanding the RNase E phase separation mechanism We currently propose that protein–protein interactions between RNase E’s positively and negatively charged blocks play a key role in RNase E’s homotypic phase separation Whereas in RNA-stimulated phase separation RNase E’s positively charged block interacts with negatively charged RNA This competition could be direct competition for binding to the same interaction motifs where salts may alter RNase E’s conformation to prevent multivalent interactions Delineating these models will require understanding the multivalent interaction mediating RNase E phase separation PNPase shares similarities to this example as its key substrate (phosphate) also attenuates RNase E phase separation at high concentrations RNA substrate availability is also required to form BR-bodies in vivo This collectively suggests that substrate-mediated positive and negative feedback loops may commonly regulate phase separation in cells both RNase E and SpmX biomolecular condensates are sensitive to phosphate nutrient availability RNase E phase separation is directly sensitive to phosphate levels whereas the SpmX condensates are sensitive to cellular ATP levels These studies highlight the intricate logical wiring of biomolecular condensate biochemistry and material properties with nutrient availability Plasmid pMJC0095 was constructed to express PNPase from C crescentus with an N-terminal 6x-His-tag and a C-terminal mCherry Plasmid pTEV5-PNPase-mCherry was transformed into chemically competent Rosetta (DE3) cells and plated onto LB-Miller plates supplemented with 50 mg/mL chloramphenicol 100 mg/mL ampicillin and incubated overnight at 37 °C an overnight 60 mL LB-Miller culture (30 mg/mL chloramphenicol 50 mg/mL ampicillin) was inoculated and incubated at 37 °C 6 L of LB-Miller media (30 mg/mL chloramphenicol 50 mg/mL ampicillin) was inoculated with 6 mL of the saturated culture and grown to mid-log phase (~ 0.5 OD600) Expression of PNPase-mCherry was induced with 333 µM isopropyl-β-d-1-thiogalactopyranoside (IPTG) for 4 h at 25 °C The cells were collected by centrifugation at 4 °C The resulting pellet was washed with 60 mL resuspension buffer (50 mM Tris pH 7.5 500 mM NaCl) before being pelleted again at 4 °C The cell pellet was thawed on ice and then resuspended in 10 mL lysis buffer per liter of culture (20 mM Tris HCl pH 7.5 200 U benzonase) supplemented with SigmaFast protease inhibitor tablets (Sigma) The cell suspension was lysed by continuous passage through an Avestin Emulsiflex-C3 at 15,000 psi for 15 min at 4 °C Cell debris was pelleted by centrifugation at 29,000g for 45 min at 4 °C The supernatant was loaded onto a HisTrap FF column (GE Healthcare) and washed with 20 column volumes of wash buffer (20 mM Tris HCl pH 7.5 Then was eluted with elution buffer (20 mM Tris HCl pH 7.5 Fractions containing PNPase were supplemented with 50 mM sodium phosphate (pH 7.5) at 37 °C for 1 h to drive phosphorolysis of co-purifying RNA The fractions were loaded onto a G-Sep™ 6–600 kDa Size Exclusion Columns (G-Biosciences) and eluted with storage buffer (20 mM Tris HCl pH 7.5 Fractions containing PNPase were concentrated using 50,000 MWCO Amicon centrifugal filters to 15.5 mg/mL and flash-frozen in liquid nitrogen and stored at − 80 °C Cells were thawed on ice and then resuspended in 10 mL lysis buffer per liter of culture (20 mM Tris HCl pH 7.5 0.1% Triton X-100) supplemented with SigmaFast protease inhibitor tablets (Sigma) the purification was eluted with elution buffer (20 mM Tris HCl pH 7.5 Fractions containing RNase E were loaded onto a G-Sep™ 6–600 kDa Size Exclusion Columns (G-Biosciences) and eluted with storage buffer (20 mM Tris HCl pH 7.5 Fractions containing RNase E were concentrated using 50,000 MWCO Amicon centrifugal filters to 18.6 mg/mL flash-frozen in liquid nitrogen and stored at − 80 °C MBP-RNase E-CTD-eYFP was purified similarly to RNase E-CTD-eYFP through the nickel affinity chromatography step After MBP-RNase E-CTD-eYFP was eluted from the HisTrap column the protein was desalted using a PD-10 column (GE healthcare) with heparin column binding buffer (20 mM Tris HCl pH 7.5 10% glycerol) before passage over a heparin column (Cytiva) MBP-RNase E-CTD-eYFP was eluted with heparin elution buffer (20 mM Tris HCl pH 7.5 Protein was then buffer exchanged into storage buffer (20 mM Tris HCl pH 7.5 concentrated using 50,000 MWCO Amicon centrifugal filters to 23.5 mg/mL and flash-frozen in liquid nitrogen before storage at − 80 °C to which was added protein and poly(A) at the necessary concentrations specified in each experiment Imaging samples were pipetted into a 1 mm well formed by an adhesive spacer (Electron Microscopy Sciences) affixed to a microscope slide (VWR) and sealed with a glass coverslip (VWR) Slides were inverted and allowed to sit at room temperature for 30 min before imaging on a Nikon Eclipse Ti-E inverted microscope with a Plan Apo-(lambda) 100×/1.45 oil objective and 518F immersion oil (Zeiss) Excitation filter cubes CFP/YFP/mChy (77074157) and Cy5 (77074160) from Chroma and emission filter sets CFP/YFP/mChy (77074158) and Cy5 (77074161) from Chroma were used for fluorescence imaging with a Spectra X light engine from Lumencor Images were taken with an Andor Ixon Ultra 897 EMCCD camera Images were analyzed with Fiji using a gaussian blur image subtraction and Renyi Entropy threshold method to find droplet boundaries The mean signal from each droplet area was divided by the mean signal of the non-droplet area to give a partitioning ratio for each droplet which is averaged to give a partitioning ratio for each experimental condition Droplet circularity was measured in Fiji using the formula circularity = 4 * π * (area/perimeter2) the tolerance and Z-score were set to 160 and 18 The ionic strength of solutions was calculated using the following equation: Turbidity reaction mixtures contained 20 mM Tris pH 7.5 The magnesium titration trial contained 4 mM PO4 pH 7.5 and the phosphate titration trial contained 20 mM MgCl2 The absorbance at 340 nm of triplicate samples was recorded with a Tecan M200Pro microplate reader (Tecan Group Ltd.) A background sample containing only buffer was collected and subtracted from each sample Fluorescent polynucleotides were generated using 5 µM PNPase with 99 µM NDP and 1 mM ADP-Cy5 in a buffer of 20 mM Tris pH 7.5 Reactions were run for 2 h at room temperature Fluorescent polynucleotides were purified using silica gel spin columns and frozen at − 80 °C for future use RNA degradation assays were performed at room temperature in 20 mM Tris–HCl (pH 7.5) and 0.5 mM DTT with 5 µM purified PNPase and 20 µM purified RNase E Reactions were initiated by adding 25 ng/µL poly(A) RNA aliquots were withdrawn and quenched in 100 mM EDTA Samples were denatured in 1.5 volumes of 2 × RNA loading buffer containing 95% formamide and 0.025% SDS and incubated at 95 °C for 3 min Quenched ribonuclease reaction aliquots were loaded onto a pre-run 6% acrylamide gel containing 7 M urea The gel was run in 1 × TBE (89 mM Tris base 89 mM boric acid) at 250 V at room temperature to separate RNA the gel was rinsed in Milli-Q water for 5 min and stained for 20 min with 1 × SYBR gold nucleic acid stain (Invitrogen) in 1 × TBE Each gel assay included RNA-only and protein-only controls Gels were imaged with BioRad ChemiDoc™ MP imager using SYBR gold settings and quantified using the BioRad ImageLab software package The intensity of the protein-only lane was subtracted from each timepoint lane intensity and plotted against time The degradation rate was calculated in relation to a known amount of RNA added in an RNA-only control lane and divided by the amount of protein to give rates in µg min−1 (mg PNPase)−1 nuclease activity cannot be reported on a molar scale The 10 C-terminal residues of Caulobacter crescentus RNase E (EKPRRGWWRR) (GWW peptide) were synthesized by GenScript with C-terminal amidation and dissolved in Milli-Q water and frozen at − 80 °C until further use In RNA-degradation assays containing GWW peptide the peptide was added to the reaction tube with buffer and PNPase and incubated at room temperature for 30 min to allow the peptide to associate with PNPase Reactions were otherwise carried out as described previously Graph-pad Prism 9 software for Mac was used for statistical analysis The analysis of variance (ANOVA) test was used to evaluate the significant differences between conditions where a p ≤ 0.05 was considered significant Data for enzyme rate analysis represents the mean and standard deviation of three replicates Data for partition ratio analysis represents the mean and standard deviation of at least 18 images taken from at least three replicates Tukey’s multiple comparisons test was used to determine significant differences between groups The datasets used and/or analyzed in this study are available from the corresponding author on reasonable request Fare, C. 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Science 356, 753–756. https://doi.org/10.1126/science.aaf6846 (2017) Download references wrote the manuscript in consultation with J.M.S The authors declare no competing interests Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41598-023-39565-w Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing newsletter — what matters in science Talent agency makes promotions in TV and sports departments Creative Artists Agency announced the promotion of four to agents or executives on Friday Brandon Lawrence has been promoted to agent in CAA’s TV department while Ashley Feagan has been promoted to agent in CAA Sports’ Talent Sales group where she will work closely with the agency’s Basketball clients Robbie Cohen and Jesse Tomares have been promoted to executives in CAA Sports Consulting manages and activates sponsorship rights deals on behalf of 30 global brands and Tomares are based in the agency’s New York office Counties across the country are now "open for solar business." This phrase sums up the Department of Energy-funded SolSmart program's core mission to help local governments adopt the most efficient policies so the booming solar industry can deliver to our communities The changing industrial and technological landscape has driven down the costs of solar electricity generation in recent years but “soft costs” to pay for business processes or administrative delays make up approximately 64 percent of these expenses Reducing these costs by streamlining bureaucratic requirements can save time and money for community members NACo has written before about SolSmart’s cost-free technical assistance driven by a dedicated support team and templates for adopting best practices the team helps our counties to find the appropriate pathway and to map the needed steps to carry your community to Bronze Gold and - newly – Platinum designations reflective of the level of simplified administrative burdens Every SolSmart designee develops a solar landing page that provides all the relevant resources and information for solar installation community members will not do their best work in the dark These pages greatly simplify the work of studying permitting For another example, you can visit the Chester County, Pa. site here Chester County staff have done commendable work reviewing zoning ordinances offering planning and zoning training and making available resources that include a map showing over 2,000 solar installations by location and municipality If you're interested in joining them and beginning, resuming or continuing your county’s SolSmart journey, click here or connect directly with NACo's team at SolSmart@naco.org.  Commissioner Melody Villard and Economic Recovery Corps Fellow Sasha Nelson discuss the transition Moffat County planned to diversify its economy after the closure of coal mines and a power plant decimated its property tax revenue Rural counties are working with their youth to create economic diversification plans and build a community that young people want to stay in — communities that have historically been reliant on coal production — involved high school students in their work with NACo’s Building Resilience in Coal Communities initiative which helps rural counties revitalize and diversify their economies amid a declining mining industry Contact Us Biomolecular condensates are membraneless hubs of condensed proteins and nucleic acids within cells which researchers are realizing are tied to an increasing number of cellular processes and diseases Studies of biomolecular condensate formation have uncovered layers of complexity including their ability to behave like a viscoelastic material the molecular basis for this putty-like property was unknown Through a multi-institution collaboration, researchers at Washington University in St. Louis, St. Jude Children’s Research Hospital and State University of New York at Buffalo examined the interaction networks within condensates to better define the rules associated with their unique material properties. Published in Nature Physics the results quantify the timescales associated with these interactions explaining why condensates act like a molecular putty and how they can “age” into a viscoelastic solid more akin to a rubber ball “Condensates have often been described as liquid-like, but their material properties can actually vary quite a bit,” explained Tanja Mittag, of the Department of Structural Biology at St. Jude, who collaborated on this research with WashU’s Rohit Pappu “That depends on the sequences of the proteins within them and the lifetime of the interactions being formed.” Beare Distinguished Professor at the McKelvey School of Engineering Jude and partners at Buffalo to establish how condensates act as reaction hubs to organize biomolecules in cells spatially Current efforts build upon years of research into the “molecular grammar” of biomolecular condensates the rules that dictate how molecules organize themselves through the process of phase separation The group further probed how condensates age changing their material properties over time Visit the St. Jude Children’s Research Hospital website to learn more Alshareedah I, Borcherds WM, Cohen SR, Singh A, Posey AE, Farag M, Bremer A, Strout GW, Tomares DT, Pappu RV, Mittag T, Banerjee PR. Sequence-specific interactions determine viscoelasticity and ageing dynamics of protein condensates. Nature Physics, July 2, 2024. https://doi.org/10.1038/s41567-024-02558-1 This work was supported by the US National Institutes of Health through grants R01NS121114 (T.M Jude Children’s Research Collaborative on the Biology and Biophysics of RNP Granules (P.R.B. acknowledges support from the US National Institutes of Health (T32 EB028092) We thank George Campbell from the Cell and Tissue Imaging Center at SJCRH which is supported by SJCRH and NCI (grant P30 CA021765) for assistance with the DIC and confocal microscopy We acknowledge the Washington University Center for Cellular Imaging (WUCCI) which is supported by the Washington University School of Medicine The Children’s Discovery Institute of University and St Louis Children’s Hospital (CDI-CORE-2015-505 and CDI-CORE-2019-813) and the Foundation for Barnes-Jewish Hospital (3770 and 4642) Research Wire Aging Biology Medicine & Health Neuroscience & Memory Read more stories from McKelvey School of Engineering Visit McKelvey School of Engineering Child tuition benefit webinar offered Flags lowered in memory of Pope Francis WashU moves training to Workday Learning Biology students win annual awards McKelvey Engineering honors 2025 distinguished alumni Student Life wins best newspaper honor at Missouri College Media awards Brad Warner, professor of pediatric surgery, 66 Julian Fleischman, associate professor emeritus, 91 William D. Owens, MD, professor emeritus of anesthesiology, 85 Understanding genetic factors behind a pediatric brain tumor Study examines overlap in causes of cancer, neurodevelopmental disorders Pollina honored for innovations in neuroscience 05.05.25 04.28.25 04.14.25 Wilmetta Toliver-Diallo Jhan Carlos Salazar Juliane Bubeck Wardenburg Who Knew WashU? 1.27.21 Who Knew WashU? 1.13.21 Who Knew WashU? 12.9.20 Defending champion Primoz Roglic suffered a nasty fall as Denmark's Mads Pedersen won stage 16 of the Vuelta a Espana at Tomares in Andalusia on Tuesday.  Aiming for a fourth consecutive Vuelta win Roglic launched an attack on the flat stage hoping to pick up bonus seconds to close the gap on race leader Remco Evenepoel less than 3km out Evenepoel was hit by a puncture as Roglic looked set to eat into his 1min 34sec overall advantage.  But one hundred metres from the finish line Roglic swerved and fell hard with his left knee and hand covered in blood Evenepoel suffered a stressful few minutes awaiting a decision on timing and expressed relief as Roglic gained only eight seconds.  "I hope Primoz can carry on," said the Belgian Vuelta rookie "I knew he would try and I'm sad for him." The 22-year-old enjoyed a lead of almost three minutes at one point earlier in the race but Roglic took back time on two mountain stages over the weekend Pedersen's win extends his large advantage in the race for the sprint green jersey on a day when the peloton raced at slower than 40kph on a sweltering day in southern Spain.  Pedersen also expressed concern for Roglic after seeing him stricken on the road just behind the finish line.  "I wanted to go to him here and I saw he was with destroyed clothing," he said I hope it isn't too bad so he can continue competing for the victory of the Vuelta."  Stage 18 and 20 feature multiple mountains when Roglic and Enric Mas Tuesday's stage ends with a 10km climb at a five percent gradient to an altitude of 2,512m which creates the possibility of a mini shake-up Get the most important Cycling stories delivered straight to your inbox By Hubert Walker for CoinWeek … construction workers in the town of Tomares a suburb of the southern Spanish city of Seville discovered a hoard of almost 1,323 pounds (600 kg) of ancient Roman coins workers were performing routine work on water pipes near Zaudín Park in Tomares when they noticed “irregular terrain” in one of the ditches they had excavated At a depth of about one meter (slightly more than one yard) the workers uncovered 19 amphorae – clay vessels used to carry or store grain olive oil and a wide variety of other products – of a small type that was specifically used to store treasure The vessels–10 of which were intact–were brimming with coins a spokesman for the Andalusian Department of Culture said that the coins and their containers were “deliberately concealed underground with a few bricks and a ceramic filter” Much like other nations rich in cultural and historical artifacts Spanish authorities follow a protocol when such a discovery is made and archaeologists assisted by the Andalusian government began to investigate and remove the amphorae from the site The Archaeological Museum of Seville (Museo Arqueológico de Sevilla) is now in charge of the find The Romans maintained a presence in Spain and the Iberian Peninsula for approximately 600 years from the beginning of the Second Punic War in 218 BCE to the early fifth century CE when a Germanic tribe known as the Visigoths succeeded in forcing a Roman withdrawal the country of Spain gets its name from the Roman province of Hispania The coins recovered last week–unusually homogenous in type for such a large find–date to the late third and early fourth centuries CE Most (but not all) feature the emperors Maximian (ruled 286-305) and Constantine I (“the Great”; ruled 306-337) on the obverse Each emperor’s coinage would have found its way to the Iberian provinces for different reasons Maximian led Roman expeditionary forces into Spain against North African raiders between 296 and 298 but later retired to Massalia (modern-day Marseilles) in Gaul where he died under suspicious circumstances in 310; Constantine reunited the Eastern and Western portions of the Empire after a period of rebellion A variety of images of a mostly allegorical nature grace the coins’ reverses The money is believed to have been intended either to pay soldiers and civil servants or to pay imperial taxes The coins–almost all of them covered in a green coppery patina–are in exceptional condition considering their age The lack of wear leads experts to conclude that they were freshly minted and had yet to enter circulation They were most likely minted in the eastern part of the Empire (in Greece The majority of the coins are made of bronze head of the Archaeological Museum in Seville said that the find is “unique for Spain” and possibly the world she has consulted with colleagues from institutions in Britain who all emphasize the importance of the find for the academic study of the historical period under consideration The museum’s Cultural Department says that no coins like the ones recovered last week already reside in their collection Once the site investigation is complete and the coins have been processed they will go on display in a permanent exhibit Navarro would not give a more precise estimate she was willing to state that the “priceless” treasure was worth at least “several million euros” The exact number of coins has yet to be counted http://www.abc.net.au/news/2016-04-29/roman-coin-cache-found-in-southern-spain/7369272 http://www.wsoctv.com/news/spain-park-workers-hit-on-huge-roman-coin-trove-by-accident/248506046 http://www.smithsonianmag.com/smart-news/spanish-ditch-diggers-unearth-1300-pounds-roman-coins-180958958/?no-ist http://www.thelocal.es/20160428/building-work-unearths-600kg-of-roman-coins-in-spain https://www.washingtonpost.com/news/morning-mix/wp/2016/04/29/construction-workers-in-spain-unearth-huge-trove-of-ancient-roman-coins/ http://elpais.com/elpais/2016/04/28/inenglish/1461850815_152135.html http://www.dailymail.co.uk/sciencetech/article-3565350/Spain-Park-workers-hit-huge-Roman-coin-trove-accident.html and website in this browser for the next time I comment This site uses Akismet to reduce spam. Learn how your comment data is processed. Image courtesy of Consejeria Cultura de la Junta de Andalucia You never know when an everyday situation can turn historic Such an event occurred in April in Tomares Spain — a small town about five miles from Seville — when a team of construction workers installing electricity in a park unearthed an incredibly large haul of Roman coins More than 50,000 coins were found in 19 jug-like vessels called amphoras The actual weight of the haul was over 1,300 pounds (590 kilograms) The workers halted their project when they realized what they had come across It is believed that the machines used during the construction caused some of the breakage among the 10 shattered amphoras but feature a small percentage of silver — less than 4 percent in very good condition of conservation,” Ana Navarro director of the Museo Arqueológico de Sevilla When were the coins stashed away underground “We have been able to read for sure inscriptions for Diocletianus Maximianus and Maxentius; almost certainly for Constantius I and probably for Severus II we could date this hoard to Tetrarchic times a political system established by Diocletian [in] 293 to govern the vast Roman Empire that [lasted] until [the early third century] … when Constantine I defeated Majentius at the battle of the Milvian Bridge.” These bronze coins were of the follis denomination they appear to have a higher silver content than they actually do and still gives a shinier look from certain angles There’s still plenty to learn about the history behind these coins What we do know is that this was a unique time of Rome’s rule The Tetrarchy period saw four men rule with equal amounts of power The thought behind this method was that the government would be able to handle issues from all corners of the empire without a ton of strain on one ruler During this era, millions of coins were struck, under the names of 15 different people. Coins like the ones found in Tomares are readily available. They feature simple art, and are neatly struck. Most collectors liken them to a fine wine of sorts; they’re an acquired taste. Navarro said that “only hypothesis and mere conjectures” can be offered as to the reason for the hoard An “in-depth archaeological and numismatic study” will hopefully turn up more info World Coins Roglic attacks but crashes as Evenepoel punctures in final 3km Behind, red jersey Remco Evenepoel (QuickStep-AlphaVinyl) suffered a puncture meaning that he would be handed the same time as the main peloton when the commissaires reviewed the finish Roglič led the five-man move all the way through the finale in Tomares dragging them into the finishing straight with eight seconds in hand on the bunch what looked like a successful day’s work for Roglič took on a different guise in the closing metres when he crashed heavily after a touch of wheels with Wright but he sat at the roadside in some distress after the finish He was still there when Evenepoel came home almost three minutes later following his puncture and the youngster went to check on Roglic before making his way to the podium After a wait, the commissaires confirmed that Evenepoel had been awarded the same time as the peloton, meaning that he limited his losses on Roglic to eight seconds. In the overall Vuelta standings “I wasn't in the best position because we did a recon of the final yesterday in the last 4 or 5km so I lost some positions and I wanted to move up on a steep bump I felt I had a flat tyre,” Evenepoel said “I'm happy that the 3km rule exists otherwise I would have lost a lot of time today I heard that Primož crashed so I really hope that he's OK and that he can continue the race.” was a resounding winner in the sprint for the stage The Dane has all but secured the green jersey thanks to his second stage win of the race even if the day will be remembered for the late drama involving Roglic Pedersen had not anticipated Roglic’s move “Ackermann was straight on his wheel I hope it’s not too bad so he can keep contending for the victory of the Vuelta.” After the final rest day of the race, the Vuelta caravan assembled on the left bank of the Guadalquivir River for the start of stage 16 in the sherry-producing town of Sanlúcar de Barrameda. Maxim Van Gils (Lotto Soudal) was absent after testing positive for COVID-19 on Monday while Esteban Chaves (EF Education-EasyPost) also withdrew before the start but the rest of the 142 riders in the peloton were given a clean bill of health to continue in the race Not many riders were much inclined to race in the opening kilometres of a long and largely flat stage at this juncture in the Vuelta Ander Okamika (Burgos-BH) and Luis Angel Maté (Euskaltel-Euskadi) attacked more or less as soon as the flag was dropped outside of Sanlúcar and they were given plenty of early leeway by the bunch They quickly built a lead of four minutes before Cofidis and Trek-Segafredo set about policing the head of the peloton on behalf of Coquard and Pedersen There was precious little change in that status for most of the long where the only statistical interest was provided by Maté who has pledged to plant a tree for every kilometre he spends off the front on this Vuelta The break’s lead dropped to a minute or so by the time they reached the intermediate sprint in Alcala del Río with 28km to go where Pedersen cruised through in third place to add another 15 points to his advantage in the green jersey standings Okamika and Maté’s day-long adventure came to an end with 14km or so remaining just as the terrain grew a little hillier on the approach to Tomares Evenepoel’s QuickStep-AlphaVinyl squad positioned the red jersey towards the front from there on with Ineos Grenadiers and Jumbo-Visma also moving up The pace on the short unclassified ascent with 12km to go was brisk but not intense enough to burn off the fast men, though Kaden Groves (BikeExchange-Jayco) saw his hopes of victory torpedoed when he punctured over the other side The stage took on altogether different guise inside the final 3km The combination of that surprise effort and Evenepoel’s puncture seemed to be tilting the balance of this race back in Roglic’s favour but the picture is muddled after his crash in the finishing straight who had led the break into the closing metres was drifting back when he appeared to make contact with Fred Wright I was blocked by Primoz and I think that’s what caused the crash,” Wright said “I think it was just a racing incident I was coming out and around and he was coming backwards Results powered by FirstCycling Doctors recommend against staring directly at the sun but the latest news in solar power may be worth a look While costs associated with solar power are declining growing government and private sector investment is helping manufacturing scale up and contribute to technological breakthroughs Those successes are driving more investment and more research the cost of electricity from solar has dropped by 87% and the cost of battery storage has dropped by 85% Prices today are already lower than two-year-old predictions for 2030 are responding to this growing affordability Thurston County’s senior program manager for climate mitigation was optimistic about integrating solar capacity into county property Panels have already been installed at the county’s public works facility and the county is considering other potential solar projects “There are a lot of opportunities for how a local government can use its own space — including the land and rooftops we own and control — to maximize the benefits of clean Those benefits go beyond reduced carbon emissions Embracing solar power can bring new jobs to a community lower residents’ energy bills and offer greater energy security by diversifying power sources Department of Energy SolSmart program offers support to counties exploring solar power SolSmart offers no-cost technical assistance to reduce costs and barriers for solar installations for local governments Their training and resources are available to all communities whether they have experience with solar power or not counties are learning how to reduce non-hardware “soft costs,” which can account for two-thirds of the costs of going solar Their expert guidance helps counties effectively streamline processes cut red tape and reduce installation delays and expenses Meeting targets for cost-reduction standards can see a community designated “bronze gold or platinum” which pertains to each community’s level of solar commitment SolSmart has already designated 506 communities achieving a silver designation earlier this year and Harvey attributed guidance and support from SolSmart for that achievement SolSmart’s support in setting and achieving goals built up internal knowledge and confidence building momentum for the county’s next steps “[SolSmart] really does make it easy,” Harvey noted They have developed the program to be clear and prescriptive in telling you what steps to do but also open enough that if you have unique stories to tell or unique situations in your jurisdiction Sharing information within the county and across jurisdictions has been a key to Thurston County’s success.  “One of the [SolSmart] requirements to achieve designation is to develop a solar landing page on your website,” Harvey shared it’s very easy to share information such as state laws Thurston County and three of its cities — Olympia Tumwater and Lacey — collaborated to produce a multijurisdictional climate mitigation plan avoiding duplication of effort and prioritizing proven cost- and time-saving measures Two of those cities are themselves SolSmart designated Olympia receiving Gold in 2022 and Tumwater Silver in 2023 For help getting started with solar power, contact NACo’s Resilient Economies and Communities team at SolSmart@naco.org.  SolSmart is a national program that helps cities counties and regional organizations become solar energy ready Communities rose all over the country to help build modern America those places became home for the people who made that work possible In the modest Sevillian suburb of Tomares, a three-year-old Juan Larios decides he wants to play football against the wishes, initially at least It does not take long for him to become utterly engrossed Larios begins setting up a makeshift goal inside the family home he would see his older cousins already playing for the local club He soon wanted to go with them to practice Despite his tiny frame — he is only 5ft 5in (165cm) now — Larios started out as a goalkeeper of his larger-than-life character from an early age He would dive around with unrelenting enthusiasm Even if his love of football refused to dim Especially after banging his head on a post countryman Jordi Alba was beginning to emerge as Europe’s best in that position winning Champions Leagues and European Championships Generation Z footballers who moved to England as teenagers in the summer of 2020 The Spaniard’s switch to left-back was transformative Larios was scouted and invited to train with Sevilla’s academy at the age of seven He would stay at his hometown club for four years before the giants of Barcelona spread their net east The thought of moving 10 hours away from the family home and more than 950 kilometres admittedly came with trepidation the overriding feeling was that he would adapt Larios was and still is an extreme extrovert popular and talkative with those who come into conversation with him there were few doubts he could make new friends and flourish in the eminent La Masia academy He lived in a dormitory on his own within La Masia despite the grounds housing around 60 players remaining at left-back but developing the technical and tactical aptitude required to play at the highest level He was courted by Spain’s youth teams at under-15 level before stepping up to their under-16 and under-19 sides His understanding of the position tallied with a maturity beyond his years “It (moving away from home aged seven) leads to a very quickly grown person,” Southampton manager Ralph Hasenhuttl tells The Athletic. “He has a character that you can feel there are a lot of things happening in his life since being here and in a positive way This is what you can feel when you talk to him They beat competition from Manchester rivals City signed Larios for a fee worth in the region of £400,000 ($450,920) The deal to bring Larios to England was negotiated through intermediaries and finally completed in September 2020 following protracted discussions throughout the transfer window His family recognised his determination and were reluctant to stand in his way No aspect of the move was thought to have fazed Larios — even in the isolated circumstances of relocating to a different country during the restrictive times of COVID-19 he was used to spending long stretches on his own and away from family Larios initially joined City’s under-18s before moving to their Elite Development Squad (EDS) at the start of the 2021-22 campaign He maintains a good relationship with Pep Guardiola and credits him for playing a key role in his development Larios transitioned to right-back on occasions including the game against Mansfield Town in the Papa John’s Trophy two days before joining Southampton as well as his ability to counteract his physical disadvantage — such as his height — through increased aggression and confidence will only be positive for his future career possibly more than any other manager in modern football has shaped the role of a modern full-back and how they are perceived The City manager has given the position increased responsibility to where the onus is on a full-back to influence games from an attacking standpoint They are tasked with taking up inverted positions in midfield and performing within rotational patterns of play Larios can only have benefitted from such principles Larios spoke to Lavia before deciding to sign who has established himself as the poster boy of Southampton’s distinctive youth model expressed the positives of making the move The teenager subsequently signed a five-year deal on deadline day along with City team-mate Samuel Edozie They were Southampton’s third and fourth summer signings from the Etihad with Edozie spending three seasons in Manchester was one of the men responsible for bringing the winger to the club having first started tracking him at the age of 12 The combined fee for Larios and Edozie was £16million as City inserted various contingents such as a buy-back clause sell-on clause and matching rights — where they can match any offer Southampton receive for either player Larios has acclimatised well to life on the south coast who was in England in the early weeks after signing to help his son get settled to frequently commute between the family home in Seville and Southampton Dani has been married to Larios’ mother for most of her son’s life so he has been a key part of his childhood ⚡️ @juan_larios7 pic.twitter.com/ushomTfpeY — Southampton FC (@SouthamptonFC) September 7, 2022 Dani was in attendance for Larios’ Premier League debut against Aston Villa where there was an element of surprise at seeing him come on at half-time and play the second period such as his fondness of the British weather Though comparisons between Seville and Southampton are scarce the two cities are similar in where they are situated in their countries Both are on the coastline and southernly — Larios can continue to enjoy living near the sea and a general sense of tranquillity The benefits of moving to Southampton are thought to have outweighed a notoriously busy Manchester city centre where his outgoing but level-headed personality can easily steer clear of distraction Larios returns to the club he left a little more than a month ago Both Southampton and City continue to think highly of him with his current boss likening the 18-year-old to illustrious company “He reminds me of a very famous player in Germany,” says Hasenhuttl “I played with him when he was 18 or 19 in the second team of Bayern Munich — it was Philipp Lahm He (Larios) is a little bit similar to him He is very clear in his mind and very clear in what he wants to do and what he wants to achieve This is what you need in modern football.” (Top photo: Matt Watson/Southampton FC via Getty Images) Jacob is a football reporter covering Aston Villa for The Athletic. Previously, he followed Southampton FC for The Athletic after spending three years writing about south coast football, working as a sports journalist for Reach PLC. In 2021, he was awarded the Football Writers' Association Student Football Writer of the Year. Follow Jacob on Twitter @J_Tanswell Construction workers working on water pipes in Seville stumble upon ‘unique’ collection of bronze coins said to be worth millions of euros Construction workers have found 600kg (1,300lb) of ancient Roman coins while carrying out routine work on water pipes in southern Spain, local officials have said “It is a unique collection and there are very few similar cases,” Ana Navarro, head of Seville’s archeology museum, which is looking after the find, told a news conference. Dating back to the late third and early fourth centuries, the bronze coins were found on Wednesday inside 19 Roman amphoras, a type of jar, in the town of Tomares near Seville. Navarro declined to give a precise estimate for the value of the haul, saying only that the coins were worth “certainly several million euros”. Read moreThe coins are stamped with the inscriptions of emperors Maximian and Constantine and they appeared not to have been in circulation as they show little evidence of wear and tear It is thought they were intended to pay the army or civil servants “The majority were newly minted and some of them probably were bathed in silver because the value they really have is historical and you can’t calculate that.” Local officials have suspended the work on the water pipes and plan to carry out an archaeological excavation on the site The Romans conquered the Iberian Peninsula in 218 BC 'Bizarre' Roglič crash leaves Vuelta future in question Giro d’Italia and Vuelta a España.Get the latest with our sport podcasts on your favourite podcast apps.Watch on SBS SportSport News Fishback was nominated for “Outstanding Performance In A New Series,” following her depiction of her character Dre in “Swarm.” The dress she wore to the awards show didn’t disappoint The train on the number cascaded perfectly and didn’t overpower the rest of the look The neckline featured one-shoulder detailing exposing her shoulder tattoo and part of her decolletage The rest of the dress appears as if it was reconstructed with panels of black glistening fabric pieced together by diamond-clad ringlets Her hair was in an elevated bun and wispy bangs on the sides For jewelry Tomares opted for a dainty necklace on her neck and on her feet Last night’s Givenchy look reminds us that Fishback is a red carpet darling and one to watch when it comes to fashion her role in “Swarm” was still one of the year’s most impressive television performances Primeras imágenes del hallazgo de monedas romanas de Tomares en el #MuseoArqueologico de Sevilla pic.twitter.com/5aK5fovNjj a Spanish construction crew that was installing electricity in a park in Tomares — a town in the province of Seville — made an incredible discovery.  CNN reports that while digging the crew banged into a collection of ancient Roman bronze coins that date back to the third century.  Tens of thousands of coins were found in 19 amphoras According to the Spanish newspaper El Pais the initial hypothesis is that these coins were stashed away to pay taxes or army levies.  There's no initial information regarding the overall value of this hoard.  Scottish novelist and poet Nan Shepherd and scientist Mary Somerville will grace two new Scottish notes from the Royal Bank of Scotland starting in 2017.  "The bank says this is the first time the two notes will feature women," writes Arthur L "The theme for both notes is 'the Fabric of Nature.' They will be printed on De La Rue’s Safeguard polymer substrate and will contain a variety of security features that De La Rue says will make them hard to counterfeit but easy to authenticate." The Professional Numismatists Guild made a public service announcement recently warning the public about thecounterfeits that are running rampant right now.  If you're a serious collector, you'll want to read this Kitco.com lists the following prices per ounce Monday at 9:05 a.m Check out three interesting stories from the last couple of days: El flamenco It is made up of diverse geographies We cannot understand our culture without that uniqueness that comes from the land Some of which have a very marked distinctive feature From the San Miguel and Santiago neighborhoods of Jerez de la Frontera come Jesus Mendez y Pepe del Morao Andevalean style Peña flamenca Smuggling Almost 250 kilometres crossed from south to north of western Andalusia and in between a host of different events with marked personalities I have just returned from Paymogo and I want to tell you about the good taste that both artists have left in our mouths It was clear that Méndez and Morao had come to put their hearts into it and from the first note they have established a special communion with the audience Pepe del Morao played the sonanta por soleá so that Jesús Méndez could lead us with his usual elegance to the enjoyment of the cante great What a pain in the ass my mother / when am I going to have a mother / like the one I had the Jerez native finished his performance with soleá Rescuing the airs of The Twin y Chacon He gives us two malagueñas with no more decoration than what he himself cante requires The powerful voice of Jesus is tempered and envelops us to pinch us in one of the best moments of the night Perfectly escorted by a guitarist who oxygenates and carries the singer on his shoulders until delivering the right melodic sounds so that he finds the space and executes the cante in a remarkable way Tientos and tangos bring us to the close of the first part of the recital The siguiriya that Jesús has given us is very good The siguiriya sounds of Jerez de la Frontera appeared in his throat and the siguiriya became even richer on Pepe's strings They are feeling the stones My fatigues I wear them day and night A magical heartbeat that has moved us and made us feel Just what should be demanded of cante by siguiriyas «Pepe del Morao played the sonanta por soleá so that Jesús Méndez could take us with his usual elegance to the enjoyment of the cante "big my mother / when will I have a mother / like the one I had?'" There is no room for the slightest misstep The enormous voice of Jesus and the accompaniment of Pepe take us to the land of wine and horses and they even put the rhythm into our skin The night will be rounded off by a range of fandangos ending with an audience grateful for the dedication of a singer who as he himself warned at the beginning of the night It is difficult to find recitals by relevant artists That is why we must highlight when professionals of this stature put the spotlight on the role of flamenco associations As the artist has repeatedly said during his performance We must also acknowledge when artists of this stature visit our entities not leaving even an iota of professionalism off the stage Recital by Jesus Mendez and Pepe del Morao Peña Flamenca Smuggling 2025 Cante: Jesus Mendez Touch: Pepe del Morao I was born in Flamenco at a table of cabales of the Peña Flamenca from Huelva I do so from the heart and looking at the people Quick-Step Floors have won 15 Grand Tour stages this season a performance only five other teams have attained throughout history Matteo Trentin and Quick-Step Floors continued their incredible dominance at the Vuelta a España nabbing a fifth victory in 13 days and thus matching the fantastic achievements set earlier this year at the Giro d'Italia and the Tour de France After winning in Tarragona and Alhama de Murcia a small municipality located just a few kilometers from Sevilla Quick-Step Floors were again at the head of proceedings in the long and hot stage 13 which took the peloton from Coin to Tomares (198.4 kilometers) but Belgian powerhouse Tim Declercq did once again a tremendous job at the front keeping the quintet on a leash and making sure the gap didn't go north of 3:30 Declercq – a Grand Tour debutant – rode a relentless tempo at the front and helped at times by Eros Capecchi and neo-pro Enric Mas successfully nullified the breakaway inside the final ten kilometers Former red jersey Yves Lampaert then moved to the front and softened the peloton who stretched out the pack before the final three kilometers which included a small but leg-sapping uphill drag The penultimate Quick-Step Floors rider in the lead-out train was Bob Jungels and the Giro d'Italia maglia bianca really did some huge damage in the pack leaving around 20 riders in the main group stage 8 winner Julian Alaphilippe took over the same role he had on stage ten shutting down a late attack and positioning Matteo Trentin in the technical run-in who unleashed a ferocious sprint with 200 meters to go beating Gianni Moscon (Team Sky) and Soren Kragh Andersen (Team Sunweb) and adorning his palmares with another Grand Tour stage victory it wasn't a finish which suited me; what was on the road was different and harder than what we had in the roadbook The guys were again incredible today and rode an amazing race pushing a really hard tempo in the closing kilometers and guiding me in the final; when you have such a marvelous team by your side you have to win and I'm happy for having finished off the job today" who extended his cushion at the top of the points classification after his success in the Andalusian town Trentin – the first Italian in ten years to take at least three stage victories at a single Vuelta a España edition – also referred to Quick-Step Floors historical run this year in the Grand Tours: "I came to this race with confidence but I wasn't expecting such an incredible string of victories You don't see every day a team win 15 Grand Tour stages in a season and what we have done so far this year is something which will stand forever What is even sweeter is that the race is far from being over and we'll have more opportunities to notch up other strong results." David De La Cruz continues to be the highest-place Spaniard in the general classification of the season's final Grand Tour who posted a podium and two-10 finishes so far since the start in Nimes is fifth overall and will go into Saturday's tough stage to Sierra de la Pandera ready for what promises to be another spectacular and exciting fight in the mountains to know how many times a particular page is read We only use this information to improve the content of our website These cookies are only placed after you have given your consent the dance master passed away at the age of 82 Eduardo Serrano he earned his stage name for his dark complexion and small size He left an indelible mark of his mastery in the thousands of hours of teaching at the legendary Madrid school on Calle Love of God today on the upper floors of the Antón Martín Market run by his close friend and companion of many years Joaquin Sanjuan She began to take her first steps in dance at the Madrid academy of Antonio Marin where another large one that disappeared a few days ago was also formed the chungaBut her career took off when she joined the Ballet of the great teacher Pilar Lopez with fourteen years! Antonio Gades and Farruco (that's nothing) Eduardo always considered the sister of the little argentinian as his teacher who according to him taught him everything that made him become a great dancer flamencoWith it he won the award for best dancer of the season in 1959 at the Theatre of Nations in Paris I had the good fortune to meet him and chat with him on several occasions Patas HouseBut the day that I was a sworn judge of the Cordoba Competition Jose Manuel Gamboa and server in the The Blason Restaurant from Cordoba (El Sablon during which the two teachers told each other anecdotes about Doña Pilar’s ​​ballet as they referred to their teacher (she referred to them as “my children”) Güito boasted of arriving at the theatre five minutes before the performance while Mario needed to warm up for an hour and a half to which the Cordoban teacher replied sarcastically: “Come on you’ve been doing the same soleá for thirty years.” To which Eduardo replied: “I’ve changed some steps.” Hahaha I still crack up every time I remember that unforgettable moment Between 1971 and 1975 he formed the Madrid Trio along with Mario Maya and Carmen Mora. That remains for history. buleria por soleá that Mario and he dance close to each other Gades incorporated him into the cast of the National Ballet when he created it in 1978 hence Güito appears in the film Saura Blood Wedding His most emblematic choreography that has gone down in history is none other than his very personal dance solea with music Emilio de Diego the guitarist with more than twenty years of experience alongside Antonio Gades The slowness with which he carries the air has forever marked a way of measuring that some even call “soleá de Madrid” The illustration for this brief obituary consists of sketches drawn by the versatile French artist Jean Cocteau When Eduardo was part of the company of Manolo Caracol in Malaga and which have been preserved thanks to the fact that Güito's mother put them in a safe place The dance flamenco of man loses one of its most distinguished figures, with him a glorious stage of dance closes, unrepeatable. Thanks to Gamboa today we can enjoy reading his life, which he masterfully captured in the book El Güito The head of the flamenco! Facts and deeds of the masterMay this true great of dance rest in peace With a degree and a master's degree in musicology from the University of Vienna he has given courses and seminars all over the world he has been musical director of the Antonio Gades Company and president of its Foundation In the nineties he was director of the Deutsche Grammophon label Author of numerous educational and scientific books on flamenco He is the author of the website www.flamencopolis.com Record producer and professor at the Aula de Flamencology of the University of Cadiz of the Master of the Higher School of Music of Catalonia and until September 2017 he was Professor of flamenco from the Conservatory of Music of Córdoba He currently resides in his hometown where he continues his work as a teacher and lecturer and not like those who proliferate who seem to have been electrocuted.. I feel grateful to have enjoyed the master I recently acquired his biography written by Gamboa The English translation of this tribute must have been produced by AI and left long unedited by human agency AcademyUD Almería U19 'A' cannot drop points in race to top the table After setback against UD Tomares U19, the UDA Academy prospects host CD Vázquez Cultural this Sunday at the Francisco Pomedio ground (KO 12:30pm CEST) SPAIN - SEPTEMBER 06: (L-R) Primoz Roglic of Slovenia (injured after being involved in a crash in the final sprint) and Mike Teunissen of Netherlands and Team Jumbo - Visma cross the finish line during the 77th Tour of Spain 2022 Stage 16 a 189,4km stage from Sanlúcar de Barrameda to Tomares / #LaVuelta22 / #WorldTour / on September 06 (Photo by Justin Setterfield/Getty Images) Credit: Justin Setterfield/Getty Images Vuelta 'sh*t' without Roglič but Mas vows to take fight to Evenepoel Presentation of the IX Art Biennial Flamenco from Malaga The President of the Provincial Council of Malaga, Francis Salad, presented on the morning of Wednesday, April 2, the IX Art Biennial Flamenco from Malaga which takes place from May 27 to August 2 in 26 municipalities in the province "which demonstrates the economic effort of the Provincial Council to bring the flamenco to the greatest number of citizens,” he stressed Salado was accompanied by the director of the Andalusian Institute of Flamenco, Christopher Ortega; the Vice President and Deputy for Culture, Manuel Lopez Mestanza; the territorial delegate of the Ministry of Culture and Sport, Carlos Garcia; and several participating artists, such as Luisa Palicio, Duquende, Francisco Bonela, Isabel Guerrero, Carmen Camacho, and Rubén Lara. The graphic design of the Biennial has been entrusted to the Malaga company fussy, which has delved into the history of old festivals to rescue that typeface, now in disuse, to highlight it in the posters and graphic material for this ninth edition. The program is broad and varied, combining youth and experience. It is estimated that nearly two hundred artists will be participating in the Biennial, including Manuela Carrasco, Farruquito, Aurora Vargas, Duquende, Pedro El Granaíno, Antonio Reyes, Jesús Méndez, José de la Tomasa, Luisa Palicio, La Lupi, Bonela Hijo, and Pastora Galván, among a long list of artists. The President of the Provincial Council highlighted the four major shows that make up the main focus of the program. The Biennial will open on May 27 at the Cervantes Theater with the world premiere of The Maharani, in charge of the company of Luisa Palicio. It features guest artists such as Passion Vega, La Macanita, Carrete, Chato de Málaga and El RemacheAt this opening gala, the Biennial award will be given to the flamencologist Gonzalo Rojo for his long history in favor of flamenco. And the Biennial will close on August 2nd in the Rincón de la Victoria auditorium with a show of its own production, I am a gypsy, which takes a tour of the cantebest known of Camarón de la IslaThe cast is made up of Duquende y Peter the Granain, cante, and Farruquito, to the dance, next to the Malaga Provincial Symphony OrchestraAll arrangements and musical direction are by the composer. Jesus Bola. On July 20, in the Mijas auditorium, the Ballet Flamenco AndalusiaDirected by Patricia guerrero, will present his new production, Blessed land, whose characteristics have been explained by Cristóbal Ortega. Although Manuela Carrasco She has paced her performances, she wanted to be at the Málaga Biennial for another year and has prepared a premiere, surrounded by excellent artists. Dancing to Manuela will meet on July 26 in Ojén Pastora Galvan, Gema Moneo, Zamara Carrasco, Manuela Carrasco daughter and Saray de los Reyes. On August 1, the new Coín auditorium will host a series of top artists with their own unique identity. Aurora Vargas, Antonio Reyes, Jesús Méndez and the bailaora Manuel Carpio They will carry the purity and gypsy spirit of the flamenco. On June 28, the Biennial will bring a show to Alcazabilla Street in the traditional format of flamenco which will be played by Jose de la Tomasa, Vicente Soto 'Deafness', Juana of the Pipe and Concha Vargas. Francisco Salado also highlighted the debuts of the Malaga team Francisco Vinuesa, La Lupi, Ana Almagro, Rubén Lara and Luisa ChicanoGuest artists in various shows include Rocío Molina, Pasión Vega, Carrete, La Macanita, Manuel Liñán, Alfonso Losa, Chato de Málaga, and El Remache. Ana Fargas and Paco Javier Jimeno will be with Fallen leaves in Álora and Isabel guerrero and Hairdresser in Almáchar. For her part, the flamenco dancer Sandra Cisneros will give life to María Zambrano with Mary of my entrails in Alhaurín el Grande, while the Jerez family of The Mijita will be held at the Ronda Wine Centre. As a preamble to the Biennial, it will take place in the church of the Divina Pastora From the Capuchinos neighborhood on May 25, a flamenco mass with the canteliturgical s through the palos of the flamenco in the voice of Antonia contreras and the touch of Juan Ramon CaroThe mass will be broadcast live on Canal Sur. The day before, on Saturday, May 24, there will be a cajoneada in the Plaza de la Merced with 70 cajons played by children from Cruz Verde and Lagunillas. Likewise, the Biennial resumes the cycle Flamenco tall, from the AC Málaga Palacio Hotel, with five recitals, and collaborates with the X International Camp of Flamenco and Dance Spain for Young Values, which will take place at the CEULAJ in Mollina, as well as with the masterclasses of the International Seminar of Flamenco 'City of Malaga', in collaboration with the School of Flamenco Andalusia. On the other hand, a documentary series will be held on the five Tuesdays of July at the María Victoria Atencia Provincial Cultural Center. The following will be screened: Don Quixote in New York, with Reel; Fandango, Ana Delgado. Memories of India, Fosforito, a story of flamenco y Zambra. The Provincial Council Art Museum (MAD) in Antequera will host joint exhibitions of two great artists, David Vaamonde in sculpture and Javier Caró in photography. These exhibitions have been provided by the Andalusian Institute of Flamenco, within the collaboration agreement. And Benalauría opens the doors to the photographs of Pepe Ponce y Pablo Blanes dedicated to flamenco women. “Beyond this extensive program, I want to highlight the Biennial's continued success, having been interrupted for two editions, specifically in 2009 and 2011. We have consolidated and continued it, which is the best of news,” concluded Francisco Salado. Cristóbal García also participated in the presentation, highlighting the IAF's collaboration with the Art Biennial. Flamenco from Málaga, and Luisa Palicio, who has shown her joy at having the enormous responsibility of inaugurating the Biennial of her land with her own show. Family photo of the Director General of Culture and Creative Industries together with several artists from this edition The figure of the brilliant and innovative guitarist You know presides over the second edition of the Flamenco Guitar Festival of the Community of Madrid which is held in Teatros del Canal from April 24 to 27 Manuel valencia y David of Arahal are the stars of the four galas that make up the festival which was presented on Tuesday 4 March by the Director General of Culture and Creative Industries will be completed with three parallel days that include a round table about Sabicas two presentations on aspects of the legacy of this guitar master born in Pamplona in 1912 and died in New York in 1990 According to its director Antonio Benamargo the Flamenco Guitar Festival has come to fulfil the desire of artists flamencos in general and of guitarists in particular in a city whose fondness for flamenco sonanta is outstanding Both the artistic results and ticket sales exceeded all expectations in its first edition held in 2024 In its second date, Teatros del Canal once again becomes the stage for major concerts, which will be opened by the Sevillian Paco Jarana at the first gala of the festival on April 24 winner of the Giraldillo al toque at the 2006 Seville Biennial His career is closely linked to dance accompaniment and also to cante Since 1998 he has composed and directed the music for Eva Yerbabuena's shows the guitarist will have as guests a singer or singer and a dancer or dancer Second Falcon y Eva Yerbabuenarespectively «The Flamenco Guitar Festival has come to fulfill according to its director Antonio Benamargo the desire of the artists flamenco"The festival is a great success for flamenco guitarists in general and for guitarists in particular in a city with an outstanding fondness for flamenco guitars Both the artistic results and ticket sales exceeded all expectations in its first edition held in 2024" The second gala will feature the also Sevillian Dani de Moron Giraldillo at the Seville Biennial in 2012 Dani is one of the guitarists flamencos most awarded of the last decades which definitely took off after the maestro Paco de Lucía noticed his virtuoso qualities Constant in his evolution as an artist are the restlessness the encounter and transfer with other sounds and instruments typical of music beyond the flamenco His recordings include the albums U-turn (2012) The sound of my freedom (2015) 21 (2018) and Believe to see In 2022 he presented at the Nimes Festival Carte blanche At his Guitar Festival concert he will sing The Tremendous and will dance Patricia guerrero The guitar of Manuel of Valencia will ring on Saturday 26th with the cante from the Sevillian Hope Fernandez and the dance of the Granadan Manuel Linan He grew up in Valencia under the guidance of his most trusted guitar instructor 2014 marked a turning point in his artistic career when he was awarded the Giraldillo Revelación prize at the Seville Biennial Two years later his first album as a concert artist was released an audiovisual trilogy in which he addresses his own musical and artistic transformation bringing into play the different disciplines of flamenco guitar such as accompaniment to cante and to dance and concert guitar Black earth is his most recent audiovisual work Privacy Policy Agreement * I agree to the terms and conditions of the Privacy Policy. The 30th edition of the International Flamenco Fashion Week with a 7 percent increase in visitors compared to the previous year: 60.000 people The largest international showcase of flamenco fashion has attracted visitors from all over the world. Andalusian provinces with the arrival of 183 buses from different parts of the region as well as visitors from other autonomous communities and various countries in Europe and Central America The color palette ranges from shades sand up to the paprika, green hunting, saffron green and olive green, with browns particularly prominent. Navy blue and petrol blue are also present. In contrast, fuchsia, red, white and black have had a more discreet presence in this edition. This year the highlights are: Custom fabrics with original prints, both geometric and floral. Also, gingham and stripes. The firms have opted for fabrics with a great drape, such as knit and crepe. The former provides elasticity, while the latter does not wrinkle or lose colour. Polka dots have evolved: from an orderly and perfect design to a more unstructured, irregular and smaller version. «We have celebrated 30 years of promoting and revolutionising flamenco fashion. It has been a truly international week of flamenco fashion, marked by spectacular shows and activities that have fused culture and fashion, consolidating the growth of this industry, a key sector for Andalusia» (Raquel Revuelta) In this edition, the event featured the Sevillian actress and director Paz Vega as maid of honor and the singer Pastora Soler as honorary ambassador, who also celebrates 30 years in music and performed the Andalusian anthem during the inauguration, which was attended by the mayor of Seville, Jose Luis Sanz. In addition, the iconic SIMOF model Mercedes Munoz She paraded in a train gown, designed exclusively to commemorate the 30th anniversary of the event. The model Maria Jose Suarez, the influencers Margarita de Guzman (@invitadaideal), Claudia Ula Jimenez Revuelta and Javier Hierro and the designer Jose Perea have been the official influencers of this year. In addition, Perea made a suit live with more than fifty meters of fabric, provided by Flamentex. The piece has been another of the great tributes to the 30 years of SIMOF, combining tradition and modernity. Singer Pastora Soler, the Italian model and presenter Antonia Dell'Atte, the influencer Rocio Osorno, the dancer Cecilia Gomez, The designer Elena Benarroch, the influencer Gloria Camilla, singer Rosario Mohedano and the singer Manu Tenorio, ambassadors of SIMOF 2025, They have received a statuette by the Jerez artist Cristóbal Donaire, Balcris. The Association of Fashion Designers and Flamenco Crafts of Andalusia (Mof&Art) has recognized the great legacy of the designer Just Salao, coinciding with the 20th anniversary of the association. La sevillana Lucia Ruiz Guerrero, 20 years old and from El Viso del Alcor (Seville), has been the winner of the Competition for New Flamenco Fashion Designers with your collection CoralesThe SIMOF 2025 revelation award has been awarded to Sara Pozo Moreno from Seville with Thalassa. El Aromas Award The best model of SIMOF 2025 has been awarded to the model Cristina Lopez Japan and the Aromas Crea Award It has been for the designer from Granada Antonio Gutierrez. In addition, the designer Sara Sanabria with your collection Bambola has won the SIMOF Designer Poster Competition, sponsored by Clínica Doctor Ortiz, valued at 1.000 euros. While the special mention, prize chosen by the public through social networks, was for Sara de Benitez for the poster of his collection So. In it we not only see the musician playing with magnificent companies –Remedios Amaya, Farruquito, Israel Fernandez, La Tana…–, but also displaying his intimate wounds, especially a family episode that marked his childhood, and on which the film wants to act as a sort of exorcism. “It all started when I went to a party, met Yerai, fell in love with him, and from there we started working,” says C. Tangana. “As an artist, I have always used my life. Here it is not about mine, and Yerai’s responsibility. During the shoot we depended on Yerai, on how he felt, where the story was, what hurts him, what he yearns for. I am fed up with myself, and in that sense this project has been very liberating, there was something of salvation for me in it.” “I only had one red line,” adds the singer and newly minted filmmaker, “and that was that The film was supposed to make things better for Yerai's family., and not as a simple artistic game between him and me. We couldn't afford to make things worse. When my artistic blindness prevented me from seeing it, Yerai reminded me of it.” «The poster and the images at the beginning and end of 'Yerai Cortés' Flamenco Guitar' represent the takeoff of a spaceship. It is a metaphor for the longing to connect with those heavens where the loved one who has lost lives. But it could also be read as the definitive propulsion towards stardom of an artist with a legitimate hunger for success» Along with Cortés, his parents have a prominent role in the film, Miguel y MaríaThe guitarist says he wanted to reconnect with them after a few years of distance and disagreements. “In Madrid I lead a very different life to the one I led in Alicante, and I wanted my parents to know me, to know about me. To bring me up to date with them. But it has also helped me to get to know them better.” Everything from the plans to the letters is calculated to elevate Yerai Cortés to the category of new icon, especially for the new generations of viewers. However, the musician stresses that “the protagonist of the film is not the guitarist, but the guitar and what I tell with it. The process has been amazing, a very nourishing learning process because of what everyone contributes,” he says. “I am flamenco, but in the film we wanted to tell a story.” A quarter of a century of cultural journalism Photo: Carlos Fernández - extampasflamencas.com We live in a flamenco generation in which there are very few artists who stand out for being creators when one goes to a concert-recital in which everything is new despite being half a century old one leaves grateful and with the happy conscience of having lived special moments You hear the same thing over and over again cante which seem the same but are nothing like each other the feeling of the value of the true contribution to the cante and the value of being and sounding like yourself few bulerías are as personal as those that contributed Lole and Manuel Back in the seventies giving a renewing twist to the concept of new flamenco and being pioneers in what a few years later would become an official trend Today we only have the great Lole montoya to remember those songs that have left such a mark on the new generations and that have given so much color to this cante is identical to the one she marked when she was touring with her husband Manuel Lole Montoya celebrated Andalusia Day at the Peña Flamenca The Silversmith (Granada), in an intimate recital open to the public with the guitar of the young man Manuel Angel Rojas. Packed to the rafters, the performance was presented by the young woman Alba Peña, young member of the Board of Directors. Rojas came out, taking his place as the singer's squire, who was received with a great ovation. Everything is colored opened the night. So, at first, we could think that the canteAn artist's most popular songs are often left for last, but in this case it is that Most of his works have been the soundtrack of several generations so the repertoire became a concatenation of cantes, almost all of them by bulerías and hit parade at the time. «Lole's brilliant voice is still alive, although the strength of yesteryear has given way to a thread of sensitive sweetness that tempers the rhythm of the bulerías until it stops. That is why the singer almost speaks cante» Lole's brilliant voice is still alive, although the strength of yesteryear has given way to a thread of sensitive sweetness that tempers the rhythm of the bulerías until it stops. That is why the singer almost speaks cante. So much so that Rojas sometimes had trouble closing the thirds. It is also true, and so it seemed, that there were hardly any previous rehearsals, since the closings of some cantes were independent. To finish her performance, which was not particularly long in time, she wanted to use part of the repertoire that she learned in her childhood from her teachers, her mother Antonia La Negra. A long, intense, slow, personal bulería that returns to its origins, those in which the versatile Manuel gave his own light with his poetic lyrics that crossed the soul with just three verses and told a life story in such a short meter. Lole Montoya's recital Peña Flamenca The Silversmith, Granada February 28th 2025 Cante: Lole Montoya Guitar: Manuel Angel Rojas Full capacity Education inspector and doctor in flamenco Critic flamenco and I International Research Award of Flamenco Paula Comitre. 'Apres vous, madame.' Nîmes Festival 2024. Salle de l'Odeon. Photo: Nîmes Festival The economic crisis first and then the pandemic have imposed a trend towards austere formats: if your proposal fits in a suitcase, fine. If instead of eight musicians you can get by with one, so much the better. But that does not necessarily make it easy for creators. On the contrary, it forces them to squeeze the most out of those few resources, to bet on more interesting stories and, ultimately, to get the best out of each one. After you, madam seems to respond to that requirement. The argumentative pretext is none other than to remember Antonia Merce Argentina, and the moment when she settled in Paris, then the capital of the world. A spotlight reveals the back of the Sevillian dancer, her arms begin to move to the rhythm of Bass's keys, which throughout the production will be exploring different sounds by means of a game of magnets and adhesives on the piano strings. For example, the vibration of the castanet, invisible in Comitre's hand. «Comitre develops a simply captivating dance discourse: barefoot or in heels, splendid in her feet as well as her arms, she maintains a frenetic dialogue with Orlando Bass's piano with moments of almost dizzying speed, but also with its calm passages» As the light expands, we see a vertical surface of blood red color, with a viscous texture and shine, resembling a bloody fiber, perhaps an entrail. A part of it comes off like a train of dresses, which will surround Paula Comitre's waist throughout the show. The artifact also has the capacity to be inflated and deflated through a mechanism controlled by the dancer, which allows her to play with volumes before the eyes of the spectator. Nîmes Festival 2024 Odeon Hall January 16, 2024 Dance: Paula Comitre Piano: Orlando Bass Academy2-1: First win of the season in the bag for UD Almería U19 AThe UDA Academy prospects grabbed the three points in their home debut against Tomares UD who made the match very complicated for them AcademyUD Almería U19 A to go all out for victory on Matchday 2 The Academy Rojiblancos host Tomares UD at the Francisco Pomedio stadium at 12 noon on Sunday UD Almería Under-19s 'A' seek to make up for the first defeat of the season in league opener against Córdoba CF U19 and have the opportunity to defeat Tomares UD The youngsters will play at the Francisco Pomedio stadium Both youth teams will go into the game level on points none of them have been able to collect points in their first match of the campaign Zeus Carmona's youth side showed that they had potential in their league opener they were unable to score a goal and therefore they have put in the graft in to be able to get the first points this term against rivals who last season were very close to being relegated Although it is true that in their first match of this season they put in a good performance against Cádiz CF U19 so the UDA Academy boys will have to remain focused in the match to be able to overcome their next adversaries The Local Europe ABVästmannagatan 43113 25 StockholmSweden Please log in here to leave a comment Three-time Vuelta a España champion Primoz Roglič made an audacious late attack that almost paid off with a substantial time gain on red jersey Remco Evenepoel but he crashed within sight of the finish line in Tomares paying a heavy price to take back only eight seconds Green jersey Mads Pedersen joined Sam Bennett Jay Vine and Richard Carapaz in the Brace of 2022 Vuelta a España Victories Club on Tuesday cementing his points classification lead in the process You can watch the 77th Vuelta a España at FloBikes Tuesday’s fare was for the sprinters as there were no classified climbs and only a couple of ripples near the end of 189.4 km The third and final week of #LaVuelta22 gets underway with a stage for the sprinters, between Sanlucar de Barrameda and Tomares, over 189.4 kilometers. pic.twitter.com/Jtc07cUcgk — Quick-Step Alpha Vinyl Team (@qst_alphavinyl) September 6, 2022 Fitting the pattern of the stages last week bothered to go out in the breakaway on such an obvious sprinter’s day Cofidis and Trek-Segafredo did most of the pace-making in the peloton the intrepid Spanish duo sopped up with 14 km to go With 2.6 km to go Roglič attacked on a little uphill rise Pascal Ackermann was able to follow and as other riders were making their way over to the move That was the Slovenian’s first bit of bad luck Roglič crashed after a touch of wheels with 100 metres to go The commisaires decided that the Jumbo-Visma man had only eight seconds on Evenepoel when the latter flatted Get the digital edition of Canadian Cycling Magazine Δdocument.getElementById( "ak_js_1" ).setAttribute( "value" Academy2-0: UD Almería U19 'A' nearly there following ninth back-to-back winThe youth side competing in Division de Honor Juvenil went all out from the off against UD Tomares to secure a stab at Copa del Rey youth tournament The youth side competing in 'Division de Honor Juvenil' went all out from the off at the Francisco Pomedio ground located in the town of Huércal de Almería Alberto Lasarte's boys left the game on track in the first half the teenage Rojiblancos took the lead courtesy of Valen's opener The attacker finished with his left foot after playing a one-two on the edge of the area that allowed him to be unmarked and beat the UD Tomares goalkeeper the lads from Almería slotted home the second of the game through Varó; the defender scored a thumping header through another great play down the right wing UD Tomares spurned a chance against goalie Juanca who kept a clean sheet for his team to earn all three points The UDA under-19s achieved one more victory at home to make 9 consecutive wins in the league Lasarte's side remain leaders in their tier ahead of Betis the talented Rojiblancos are first on a better goal difference There are 3 days left for the first half of the campaign to end at which time the teams that qualify for the 'Copa del Rey Juvenil' youth tournament will be announced UD Almería U19s 'A' would have access to one of those places The UD Almería youth team are travelling to Marbella to play against ninth-placed CD Vázquez Cultural within Group IV of the Division de Honor Juvenil tier Goals: 0-1 Valen (11') 0-2 Varó (18') played at the 'Francisco Pomedio' Field in the town of Huércal de Almería TOMARES – Italian sprinter Matteo Trentin claimed his third win of the Vuelta a Espana on stage 13 as Chris Froome finished safely among the chasing pack to retain his overall lead on Friday Quick-Step rider Trentin continued his dominance of the few sprint stages of the largely mountainous three-week trek around Spain by pipping compatriot Gianni Moscon and Denmark’s Soren Kragh Andersen to the line in a time of 4hr 25min 13sec I came to this race with confidence but never expected anything like this,” said Trentin “For the team the Vuelta is not finished and we are betting for more.” who is aiming to become just the third rider to win the Tour de France and Vuelta in the same year retains a 59-second lead over Italy’s Vincenzo Nibali Saturday’s stage promises another showdown between the race favourites with a gruelling uncategorised climb to end the 175km ride from Ecija to Sierra de La Pandera “It’s very difficult to predict what is going to happen in this Vuelta As we saw yesterday things can change so quickly,” said Froome “Tomorrow’s final is very steep I expect guys like Alberto Contador and Vincenzo Nibali want to make up time at this stage of the race “I expect a very aggressive ride from them.” Another summit finish also awaits on the Sierra Nevada on Sunday and Froome is expecting this weekend to be decisive in his quest to finally win the Vuelta after three second-placed finishes “Sunday’s stage is going to be even harder again up in the Sierra Nevada “For sure at the end of a really tough week we are going to see some really big time gaps in these next two stages.” – Nampa-AFP copyright in the content of this website vest in The Free Press of Namibia (Proprietary) Limited (Registration No 85/058) The unauthorized making of copies or use of this material constitutes a copyright infringment under the Namibian Copyright and Neighbouring Rights Protection Act Powered by PageSuite © 2025 The Namibian This site asks for consent to use your data We use cookies to enhance your browsing experience We use cookies to help you navigate efficiently and perform certain functions You will find detailed information about all cookies under each consent category below The cookies that are categorized as "Necessary" are stored on your browser as they are essential for enabling the basic functionalities of the site We also use third-party cookies that help us analyze how you use this website and provide the content and advertisements that are relevant to you These cookies will only be stored in your browser with your prior consent You can choose to enable or disable some or all of these cookies but disabling some of them may affect your browsing experience Necessary cookies are required to enable the basic features of this site such as providing secure log-in or adjusting your consent preferences These cookies do not store any personally identifiable data Functional cookies help perform certain functionalities like sharing the content of the website on social media platforms Analytical cookies are used to understand how visitors interact with the website These cookies help provide information on metrics such as the number of visitors Performance cookies are used to understand and analyze the key performance indexes of the website which helps in delivering a better user experience for the visitors Advertisement cookies are used to provide visitors with customized advertisements based on the pages you visited previously and to analyze the effectiveness of the ad campaigns