Connecting decision makers to a dynamic network of information Bloomberg quickly and accurately delivers business and financial information A rendering of Ingrid Capacity’s Nivala battery project in Finland 2025 at 2:00 AM ESTBookmarkSaveTakeaways NEWA battery-storage project that will become the biggest in Finland has been given the go-ahead to start construction The final investment decision, taken in late February by SEB Nordic Energy’s Locus Energy and Ingrid Capacity AB means construction in Nivala will start immediately The 70-megawatt system — which can store power for two hours — will be operational in the second half of next year Ingrid Chief Strategy Officer Nicklas Backer said in an interview Metrics details we provide single-molecule evidence that ClpX translocates substrates in two-residue steps This mechanism achieves sensitivity to single amino acids on synthetic protein strands hundreds of amino acids in length enabling the sequencing of combinations of single-amino-acid substitutions and the mapping of post-translational modifications To enhance classification accuracy further we demonstrate the ability to reread individual protein molecules multiple times and we explore the potential for highly accurate protein barcode sequencing we develop a biophysical model that can simulate raw nanopore signals a priori on the basis of residue volume and charge enhancing the interpretation of raw signal data we apply these methods to examine full-length folded protein domains for complete end-to-end analysis These results provide proof of concept for a platform that has the potential to identify and characterize full-length proteoforms at single-molecule resolution Unlike the rapid initial stage of threading the protein into the pore using electrophoretic force the unfoldase-mediated translocation of proteins back out of the pore leads to slow This method has resulted in the processive translocation of long proteins enabling the detection of single amino acid substitutions and PTMs across protein strands up to hundreds of amino acids in length We have also developed an approach to rereading the same protein strand multiple times this method enables the unfolding and translocation of a model folded protein domain for linear Schematic of the cis-based unfoldase approach on the MinION platform The roman numerals correspond to the ionic current states in b Deep spikes in the capture state are hypothesized to be transient structural fluctuations of the Smt3 domain in the pore State iii can be discerned from a transient drop in current when the ClpX solution is initially loaded into the flow cell Protein sequences are oriented from C to N These results are consistent with our model that ClpX was binding to the ssrA tag and translocating the captured protein out of the nanopore with C-to-N-terminal directionality This revealed that the main differences across the translocation signals corresponded with the positions of the tyrosine mutations along the protein strands comparing all-versus-all signal dynamic time warping (DTW) distances revealed that the sets of translocation signals generated by each unique protein sequence formed distinct clusters differentiating them from every other protein This was statistically supported by permutational multivariate analysis of variance (PERMANOVA) with P < 1 × 10−6 for each comparison after applying a Bonferroni correction These results are in strong agreement with the step size of two amino acids hypothesized from the structural studies and indicate that the tweezer experiments lacked the spatio-temporal resolution to resolve individual ClpX steps these results show that this method is sensitive to single amino acid residues This reinforces the assertion that our model has the capacity to simulate these current traces accurately in these sequence contexts a, Pipeline for PASTOR VR classification with machine learning (ML) models. b, Heatmap showing test accuracies in discriminating between all pairs of amino acid VR mutations, averaged over five random forests (n for VRs and experiments is shown in Extended Data Table 1) Transformed ionic current traces are plotted with a box around the variable regions defined by the YY segmenter The colour intensity of the boxes represents the ranking of the true class in the aminocaller’s prediction for each VR For the 5-way classification task (top box shading) the classes are the five mutations found in that protein whereas the 20-way classification task (bottom box shading) considers all possible amino acid classes the letter corresponds to the model’s top prediction Darker shades denote a more-accurate prediction indicating that the correct label ranked high in the model’s predictions Overall sequencing accuracy per read averaged about 62% and 42% for the HDKER sequence and roughly 51% and 21% for the AVLIM sequence using 5-way and 20-way classification models respectively Top box shows example trace of PASTOR-reread showing three almost-complete reread events (blue trace) Our model’s predicted signal for the PASTOR-reread sequence (pink trace) was aligned to each reread The fourth VR contains an asparagine mutation but the corresponding signal level consistently resembles aspartate in all three instances of this PASTOR-reread trace The modelled sequence was changed to contain an aspartate to reflect the putative PTM Bottom box shows the approximate region of the strand that is in the nanopore over time the accuracy for a 7-way classification task improved from 66% to 99% (against a 14% random baseline) The phosphorylation count of proteoforms is shown above the bars (proteoform ID1 contains no phosphorylations) After incubating PASTOR-phos with PKA for 1 h, we performed nanopore analysis, which found a substantial increase in ionic current in 91 of the 92 reads (98.9%) in the kemptide VR compared with the baseline (Fig. 5b and Extended Data Fig. 8a) This increase in ionic current is consistent with expectations for the negatively charged phosphoserine 361 of the 368 non-kemptide VRs and linker sequences (98.1%) showed no substantial signal changes These results are consistent with PKA activity being specific to the RRXS motif a, Working model of ClpX-mediated processing of folded proteins. The roman numerals correspond to the ionic current states in b. b, Example trace of PASTOR-titin. c, Example traces of titin translocation (state vii), with black horizontal lines denoting the mean of individual putative ClpX steps, found with the Bayesian segmentation algorithm (Methods) the 26-h CKII incubation resulted in single molecules containing as many as nine phosphorylated residues we observed characteristic signal features corresponding to the downstream VRs and YY regions (state viii) before transitioning back to an open-pore state (state ix) This similarity reflects their nearly identical primary amino acid sequences The observation of similar signals at the proposed translocation state vii between PASTOR-titin and PASTOR-D-titin despite their differences in structural stability underscores the role of the primary amino acid sequence in this process It indicates that the primary sequence is the main determinant of the translocation signal through the nanopore whereas structural variations have greater influence on the preceding unfolding state This evaluation is evidence that our model can adequately simulate these current traces in the specified sequence contexts further work is needed to explore more native protein sequences directly determining amino acid sequences from the nanopore signal (de novo sequencing) will be even more difficult and will require extensive datasets for training de novo amino acid callers this work serves as a stepping stone towards full-length protein identification capable of achieving the highest level of proteoform resolution particularly in the context of protein barcoding and PTM-monitoring applications Plasmids for analyte proteins were constructed using gBlocks (Integrated DNA Technologies) inserted into the pET–49b(+) plasmid (Novagen) a polyhistidine tag and a TEV cleavage site upstream of the sequence encoding an analyte protein The NEBuilder HiFi DNA assembly and Q5 site-directed mutagenesis kits (New England Biolabs) were used for plasmid construction Cloning was done using NEB 5-α-competent Escherichia coli cells Plasmid sequences were verified by Sanger sequencing through Genewiz Protein expression was induced overnight at 30 °C with BL21 (DE3) E coli cells in Overnight Express Instant TB medium (Novagen) Proteins were purified by immobilized metal affinity chromatography (IMAC) with TALON metal affinity cobalt resin and its associated buffer set (Takara) Proteins were cleaved with TEV protease (New England Biolabs) and further purified by reverse IMAC Purified proteins were concentrated using ultracentrifugal filters with a 10 kDa cutoff (Amicon) and stored in the short term at 4 °C or in the long term at −80 °C until use ClpP was purified by IMAC and stored at −80 °C until use protein (around 1 mg ml−1) was incubated overnight in 100 mM sodium bicarbonate buffer (pH 9.6) at 25 °C to catalyse deamidation protein was incubated with either 50,000 units per ml PKA (New England Biolabs) or 10,000 units per ml CKII (New England Biolabs) in a protein kinase buffer (10 mM MgCl2 The protein solution was used for nanopore analysis immediately after the incubation without purification 5 mM MgCl2 and 25 mM HEPES–KOH (pH 7.6) was used instead of standard cis running buffer to see if it would improve the signal-to-noise ratio The time-course degradation assay of the PASTOR-HDKER protein was performed in cis running buffer with 6 μM PASTOR-HDKER 300 nM ClpP14 and an ATP-regeneration mix (4 mM ATP 16 mM creatine phosphate and 7 units per ml creatine phosphokinase) at 30 °C Incubation was stopped by denaturing samples in Laemmli buffer at 95 °C for 5 min Samples were run on SDS–PAGE and stained with Coomassie blue to quantify the protein bands using the ImageJ software then DTW-aligned traces to two canonical presegmented traces and selected the alignment with the lowest DTW distance The max value of the N-terminal VR was multiplied by 1.4 and the max value of VR GLSARRL was multiplied by 1.2 and the minimal max was used as the max value for min–max scaling This was repeated after realigning to the canonical traces and segmenting the VRs ‘normalized’ refers to z-score normalization as in ‘normalized current’ when comparing a model signal with experimental signals The vector Xi describes the window starting at index i in the sequence The j-th index in Xi is 1 + Vc × volume(aai+j) + Pc × PositiveCharge(aai+j) + Nc × NegativeCharge(aai+j) where the functions PositiveCharge and NegativeCharge take 1 if the residue has a positive or negative charge The constants representing weights between charge and volume were determined empirically to minimize the average post-DTW distance of a training subset of protein traces to the model of their sequences we use a vector PW (parabolic weight) of length 20 containing values representing a negative The i-th index in S is then finally computed as the dot product of Xi and PW a minimum window length of 10 observations and a threshold P-value of 5 × 10−5 were used two canonical traces were segmented manually and the rest of the traces were aligned to both and then labels were assigned according to the canonical trace with the lowest DTW distance we weighted the loss inversely proportional to each label’s class representation in the training set we performed principal component analysis on the vector of its DTW distances to all VRs in the training set to reduce the size of the vector to 64 mean absolute value of the derivative and median absolute value of the derivative of the transformed signals as well as the standard deviation of the raw (unfiltered The CNN had the transformed signal as input It was trained with a stochastic gradient descent optimizer with a learning rate of 0.01 had four convolutional layers followed by a gated recurrent unit (GRU) and then a fully connected layer and was initialized with Kaiming initialization Max pooling and a ReLU activation function were applied after each convolutional layer The dummy classifier was implemented with the scikit-learn dummy classifier with default parameters To collect the results shown in Extended Data Fig. 7d,e we used a random forest without hyperparameter tuning and used 100 randomly selected 80–20 train–train splits This was necessary to estimate the accuracy well enough with a large number of rereads given the data limitation and the need to group samples in the test set To calculate the accuracy of barcode identification when using linear error-correcting codes of identifying a VR given an alphabet size We simulated the accuracy with error correction when n−k of the bits were allocated to linear error-correcting codes We did this by conducting 50,000 trials of: first encoding a random integer from 0 to 2k with a generating matrix into a message of n bits; second changing each of the n/log2(a) consecutive sets of log2(a) bits in the encoded message (to a different set of bits of the same length) to simulate misclassifying one VR; and third decoding the number with syndrome decoding We calculated p′ to be the percentage of trials in which the decoded number was the same as the original random number All PERMANOVA tests were done on the DTW distance matrix of signals using scikit-bio and 106 permutations in which case n × 106 permutations were used where n is the number of comparisons performed T and Mann–Whitney U tests were performed using SciPy Reported P values were multiplied by n if we noted that we used a Bonferroni correction All tests were two-sided unless stated otherwise and P values were considered significant if P < 0.05 Protein expression plasmids are available at Addgene Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article Custom MinION MinKNOW run scripts can be obtained from Oxford Nanopore Technologies on request techniques and possibilities to overcome biological sample complexity A critical review of bottom-up proteomics: the good A dual-constriction biological nanopore resolves homonucleotide sequences with high fidelity Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics & the Consortium for Top Down Proteomics Proteoform: a single term describing protein complexity Deciphering post-translational modification codes The language of covalent histone modifications Unlimited multistability in multisite phosphorylation systems Paving the way to single-molecule protein sequencing The emerging landscape of single-molecule protein sequencing technologies Swaminathan, J. et al. Highly parallel single-molecule identification of proteins in zeptomole-scale mixtures. Nat. Biotechnol. https://doi.org/10.1038/nbt.4278 (2018) Real-time dynamic single-molecule protein sequencing on an integrated semiconductor device Full-length single protein molecules tracking and counting in thin silicon channels Nanopore sensing: a physical-chemical approach Single-molecule protein sensing in a nanopore: a tutorial Label-free detection of post-translational modifications with a nanopore Single molecule nanopore spectrometry for peptide detection Resolving chemical modifications to a single amino acid within a peptide using a biological nanopore Real-time shape approximation and fingerprinting of single proteins using a nanopore Stochastic sensing of proteins with receptor-modified solid-state nanopores Nanopore-based measurements of protein size Electrical recognition of the twenty proteinogenic amino acids using an aerolysin nanopore Protein identification by nanopore peptide profiling Single-aminoacid discrimination in proteins with homogeneous nanopore sensors and neural networks Reading the primary structure of a protein with 0.07 nm3 resolution using a subnanometre-diameter pore Discriminating residue substitutions in a single protein molecule using a sub-nanopore Thakur, A. K. & Movileanu, L. Real-time measurement of protein-protein interactions at single-molecule resolution using a biological nanopore. Nat. Biotechnol. https://doi.org/10.1038/nbt.4316 (2018) Stochastic detection of Pim protein kinases reveals electrostatically enhanced association of a peptide substrate Sensing with nanopores and aptamers: a way forward Single-molecule site-specific detection of protein phosphorylation with a nanopore Quantification of protein glycosylation using nanopores Enzyme-less nanopore detection of post-translational modifications within long polypeptides Nova, I. C. et al. Detection of phosphorylation post-translational modifications along single peptides with nanopores. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01839-z (2024) Single molecule ratcheting motion of peptides in a Mycobacterium smegmatis porin A (MspA) nanopore Multiple rereads of single proteins at single-amino acid resolution using nanopores Controlled movement of ssDNA conjugated peptide through Mycobacterium smegmatis porin A (MspA) nanopore by a helicase motor for peptide sequencing application Herding cats: label-based approaches in protein translocation through nanopore sensors for single-molecule protein sequence analysis Structural and mechanistic insights into the bacterial amyloid secretion channel CsgG Polypeptide translocation by the AAA+ ClpXP protease machine Unfoldase-mediated protein translocation through an α-hemolysin nanopore Discrimination among protein variants using an unfoldase-coupled nanopore Bottom-up fabrication of a proteasome–nanopore that unravels and processes single proteins Energy-dependent degradation: linkage between ClpX-catalyzed nucleotide hydrolysis and protein-substrate processing Multiplexed direct detection of barcoded protein reporters on a nanopore array Structures of the ATP-fueled ClpXP proteolytic machine bound to protein substrate Substrate-engaged 26S proteasome structures reveal mechanisms for ATP-hydrolysis-driven translocation Protein barcodes enable high-dimensional single-cell CRISPR screens An atlas of substrate specificities for the human serine/threonine kinome Linkage between ATP consumption and mechanical unfolding during the protein processing reactions of an AAA+ degradation machine ClpX(P) generates mechanical force to unfold and translocate its protein substrates Differences in β-strand populations of monomeric Aβ40 and Aβ42 Yu, L. et al. Unidirectional single-file transport of full-length proteins through a nanopore. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01598-3 (2023) Exploiting protein N-terminus for site-specific bioconjugation Decarboxylative alkylation for site-selective bioconjugation of native proteins via oxidation potentials Replication of individual DNA molecules under electronic control using a protein nanopore Rebuilt AAA + motors reveal operating principles for ATP-fuelled machines Computing and visualizing dynamic time warping alignments in R: the dtw package Schreiber, J. & Karplus, K. Segmentation of noisy signals generated by a nanopore. Preprint at bioRxiv https://doi.org/10.1101/014258 (2015) The ClpXP protease unfolds substrates using a constant rate of pulling but different gears Kontogiorgos-Heintz, D., Yang, S., &amp; Nivala, J. PASTOR-sequencing. Zenodo https://doi.org/10.5281/zenodo.1271384060 (2024) Download references Berkeley) for discussions on ClpX stepping activity; J Gutierrez and others at Oxford Nanopore Technologies for discussions and for providing the configurable MinION runscript Present address: Department of Biotechnology These authors contributed equally: Keisuke Motone Allen School of Computer Science and Engineering Molecular Engineering and Science Institute The University of Washington has filed provisional patent applications covering protein rereading (K.M and J.N.) and sequence-to-signal simulation methods (D.K.-H. is a consultant to Oxford Nanopore Technologies and holds share options in the company The other authors declare no competing interests Nature thanks Aleksei Aksimentiev, David Rodriguez-Larrea and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Fraction of ClpX-mediated translocation events observed following capture events in the presence of no ATP (n = 230) ClpX-mediated translocation time in the presence of 0.5 mM ATP + 0.5 mM ATPγS (n = 7) Mean transformed current levels of YY and VR PASTOR segments There was a total of 305 PASTOR traces analyzed Depiction of the process of scaling signals to the “transformed” current described in Methods Distribution of the proportion of time (out of the total duration of the signal) spent within the manually segmented YY-dip regions This portion was used to estimate ClpX’s step size (Methods) Number of steps for each of the YY dips without back steps using Bayesian-based YY-segmentation Stepping behavior statistics when calculated with t-test segmentation method because with the different segmentation algorithm different putative backsteps were found and subsequently different dips were filtered (Methods) a, Scatter plot of various features of the uncharged VRs, with error bars denoting one standard deviation, center point denoting the mean, and explanation of the features to the right. n of VRs, traces, and experiments shown in panels a-c shown in Extended Data Table 1 Bar blot of the variance of the max value of the transformed VRs corresponding to each amino acid t-SNE map showing clustering of the pairwise DTW distance between each amino acid Plot of all the VRs corresponding to asparagine in normal conditions (left) and in conditions that catalyze the deamidation of asparagine to aspartate (right) Lines colored teal if the max value of the transformed signal <1.3 n = 81 for normal conditions and 77 for deamidation conditions Asparagine VRs are colored blue if the max value of the transformed signal >= 1.3 Asparagine VRs form a distinct cluster from aspartate and putative deamidated asparagine VRs (pPERMANOVA <1×10−6) Putative deamidated asparagine and aspartate are indistinguishable (pPERMANOVA= 0.8) Bar plot displaying mean percent of mutations that have been putatively deamidated or not (same threshold as in d Distance matrix of the DTW-distances between the aspartate and putative post-translationally modified asparagine to aspartate VRs shown in e Violin plots showing distribution of the maximum height of transformed VRs in normal and deamidation catalyzing conditions and the three other amino acid substitutions in PASTOR-VGDNY (valine for all amino acids in the window of size 20 Parabolic weighting of the values within a window by computing the dot product of the parabolic weight array and the window array Comparison between the nanopore signal of an example ionic current signal of PASTOR-TWAFH (black line) and the modeled signal generated for the same protein sequence (pink line) Model signal shown with the time axis aligned to the experimental trace using DTW Distributions of the DTW distances between the real (experimental) signal traces and the model signals of the same sequence (pink) or between the real signal traces and the model signals of 10,000 random sequences derived from the same amino acid distribution as the real sequence (orange) n of experimental traces ranges from 27 to 55 Both conditions consist of 2 independent runs The models’ performance was consistent across both standard and elevated salt conditions Accuracy in a 20-way classification when “accuracy” is defined as the correct label being in the top-N most probable classes The dummy classifier chooses one label at random Supplementary Notes 1–3; Supplementary Discussions 1–5; Supplementary Figs Download citation DOI: https://doi.org/10.1038/s41586-024-07935-7 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing newsletter — what matters in science Informationen på denna sida är avsedd för journalister press och media.Klicka ja för att komma till nyhetsrummet Klickar du nej kommer du tillbaka till mynewsdesk.com Locus Energy collaborates with Ingrid Capacity to build the largest battery energy storage project in Finland contributing 70 MW/140 MWh battery power to Locus Energy’s existing Finnish portfolio already consisting of solar- The project is situated in Nivala Municipality in the Ostrobothnia region and is estimated to be finished during 2026 The 70MW/140MWh project in Nivala municipality is the second investment in Finland in a short time following a 125 GWh wind portfolio consisting of 13 WTGs spread over three wind parks in the northwest It thus strengthens Locus Energy’s existing portfolio of wind- solar- and hydro assets in Finland and contributes with an important new flexibility and balancing function to the system creating opportunities for Locus Energy to optimize the system output “The Locus Energy portfolio in Finland now consists of wind- This enables us to work with our assets as a system using the hydro-and battery energy storage assets to balance the system as the volatile production assets of wind and solar shifts in production we can maximize the output from the energy produced creating a system premium both in terms of impact and results.” Says Mattias Söderqvist As Finland aims to become carbon neutral by 2035 the production of energy from renewable sources is believed to continue to increase rapidly As these volatile energy production assets gain more prominence in the system the demand for energy reserves will increase The battery energy storage collaboration project between Locus Energy and Ingrid Capacity will thus be an important enabler for balancing the Finnish energy system “The project in Nivala further strengthens the existing partnership between Ingrid Capacity and Locus Energy and together we will build one of the largest battery energy storage systems in the Nordics Our aim to accelerate the shift towards flexibility and grid optimization pairs well with Locus Energy’s renewable energy production and this shared vision of creating a sustainable energy future have proven an excellent foundation for a productive and impactful partnership” Chief Strategy Officer at Ingrid Capacity" The project will be located next to Fingrid’s Uusnivala 400 kV substation The system will be connected directly to the national transmission network (TSO Fingrid) and have an important function to stabilize the grid The project has started and is expected to be completed in 2026 something that is welcomed by the The Mayor of Nivala Päivi Karikumpu “We are very pleased to have Ingrid Capacity and Locus Energy’s investment in Nivala This project not only strengthens the local economy but it also significantly contributes to Finland's energy transition and grid stability We are looking forward to collaborating with both parties on this important project” The national transmission operator Fingrid emphasizes the important aspects of flexibility and resilience “As Finland’s transmission system operator Fingrid plays a key role in enabling the energy transition while maintaining grid stability We welcome Ingrid Capacity and Locus Energy’s project in Nivala as it enhances the flexibility and resilience of our grid while enabling the integration of more renewable power” says Jussi Jyrinsalo The Nivala battery energy storage system marks the second collaboration project between Locus Energy and Ingrid Capacity consists of 13 new grid scale battery energy storage systems across the south of Sweden and is planned to add an additional 196 MW of flexible capacity to the national grid in price areas SE3 and SE4 “SEB Nordic Energy continues to make significant investments in Sweden developing a pan-Nordic IPP of renewable energy The battery energy storage system in Nivala is an investment that further diversifies the Fund’s Finnish portfolio further advancing the renewable energy transition —aligned with the Fund’s mission to accelerate renewable energy solutions” says Richard Gavel For more information, please reach out to:Niklas Sörensen, CEO, Locus Energy, niklas@locus.energy +46 70 268 97 35Richard Gavel, Portfolio manager, SEB Nordic Energy, richard.gavel@seb.se + 46 70 772 32 53 has acquired the three wind parks Pirttiselkä Muntila and Leppävirta from SUSI Renewable Energy Fund II an investment vehicle managed by SUSI Partners The acquisition further strengthens the company’s portfolio of sustainable energy production assets in Finland contributing approximately 125 GWh per annum to the existing portfolio The new partnership represents the second largest battery investment in the Nordics and is a key step in strengthening Sweden’s energy system and advancing the electrification of society It will within 12 months improve the problematic power deficit situation in 13 communities in southern Sweden as the battery storage systems come online in the local grids Locus Energy är en långsiktig aktiv ägare och utvecklare av småskalig hållbar infrastruktur för energiproduktion i Norden När du väljer att skapa ett konto och följa ett nyhetsrum kommer dina personuppgifter behandlas av oss och av ägaren av nyhetsrummet för att du ska kunna motta nyheter och uppdateringar enligt dina bevakningsinställningar För att läsa mer om detta, var vänlig läs vår Integritetspolicy som berör vår behandling av dina personuppgifter, och Integritetspolicy för Contacts som berör behandlingen av dina personuppgifter från ägaren av nyhetsrummet du följer Vänligen notera att våra Användarvillkor gäller alla våra tjänster Du kan dra tillbaka ditt samtycke när som helst genom att avregistrera dig eller genom att ta bort ditt konto She met and married her husband Silvo in Finland and they emigrated to the United States she raised her five children as a single mother a testament to her “sisu,” the Finnish sense of will Irja was loved dearly and will be missed by all those she left behind Family and friends remember her infectious humor Her powerful faith in God was her foundation One of her favorite verses was Deuteronomy 33:27: “The eternal God is your refuge and underneath are the everlasting arms.” Her family will miss her arms as a refuge While Irja never called the Island her home she will be missed by her family members who do: her son and her great-grandchildren Chloe and Thea 29 at the Brandon Funeral Home in Fitchburg The Vineyard Gazette welcomes obituaries of people who have lived or vacationed regularly on Martha’s Vineyard Tuesday for publication in print on Friday Obituaries will appear online and in print Swimming sisters Rebekah and Kaija Nivala of Vineyard Haven had been planning for the challenge all winter The hundreds of laps and thousands of strokes were all in preparation for a four-mile swim from West Chop to Woods Hole this summer the girls believed that the only obstacles that could possibly hold them back would be themselves when a great white shark was spotted a few hundred yards off Gay Head in early June older sister Rebekah said that while they were disappointed they were still hopeful that they could “do something major this summer.” In their search for new inspiration they came upon the moving story of a 6-year-old boy named Jesse Jesse suffers from eosinophilic esophagitis The digestive system disorder is estimated to affect one in every 1,000 children making them susceptible to developmental delay and nutritional deficiencies Because of what the disease does to Jesse and other sufferers’ digestive systems it is only safe for them to eat certain foods Nearly every other food source can prompt a dangerous allergic reaction so they must gain additional nutrients through expensive protein-enriched formulas “Jesse represents all the children suffering from this disease,” Rebekah said He is the hopeful and smiling face of a cure for a disorder that is practically unknown outside of the medical community As unfamiliar as the disease is at the moment its prevalence in industrialized countries is increasing due to worsening environmental conditions When Rebekah and Kaija learned that there are a number of eosinophilic disease sufferers on the Vineyard they were inspired to combine their love of swimming with a service project With just over a month to plan, Rebekah and Kaija got to work. They set up a website (swimforjesse.com) contacted local newspapers and television stations they followed the words attributed to the pseudonymous Ambrose Redmoon: “Courage is not the absence of fear but rather the judgment that something else is more important than fear.” With the young faces of Jesse and so many others in mind reaching out anywhere they could for support Under a brilliant blue sky this past Saturday morning Rebekah and Kaija’s weeks of planning finally came to fruition local support for the event was outstanding Some participants — like 55-year-old triathlete Chris Forbes — were swimming to win In standing behind the Nivala sisters every step of the way the Vineyard community truly showed its commitment to encouraging young Islanders who aim to make a difference What was most touching on the morning of the event The sisters’ parents and younger siblings were on hand — cheering aided by her family’s cheers and Kaija’s accompaniment in the last leg of the race beat out the competition to become her age group’s winner of the 200-meter swim she gave older sister Kaija a big hug before climbing out of the water The main goal of Swim for Jesse was to increase awareness of eosinophilic disease and its effects A secondary goal was to raise money for the CURED Foundation an organization that supports leading eosinophilic disease research centers All of the proceeds from race registration benefited CURED offered additional financial donations to his family directly Rebekah is not sure what next summer holds for her If she is not able to return home and organize a second Swim for Jesse she hopes that another Islander will take the reins and make it an annual event the fundraiser’s future seems bright If the Nivala family isn’t directly involved the larger Island family most likely will be The MV Times comment policy requires first and last name for all comments Please select what you would like included for printing: Copy the text below and then paste that into your favorite email application Hank started working at a young age driving truck on a sugar beet farm and would go fishing on the Red River in his free time Hank left home and worked his way across the country working different jobs and living in different states They planted their roots in Hibbing and together raised a family of four girls He was a member of the Iron Workers Local 512 He enjoyed checking in and visiting with others Hank was always available to help those in need with whatever it might be He had a great outlook on life and sense of humor He joked around and kept a positive attitude right until the end of his life Hank is survived by his daughters Wanda (Dennis) Minerich Kerrie (Roy) Howell and Pamela (Aaron) Nivala brothers Duane (Diane) Vold and Marty (Phyllis) Vold grandchildren Cindy and Kristopher Rewertz Alex and Tyler (Rachel) Yost and Connor Nivala 12 great grandchildren and many nieces and nephews granddaughter Riley Nivala and great grandson Byron Carlson A Memorial Service and Celebration of Life will be held at 11:00 AM on Friday at Our Savior's Lutheran Church in Hibbing Visitation will take place one hour prior to service at church starting at 10:00 AM Interment will take place at the Maple Hill Cemetery in Hibbing.  Enter your phone number above to have directions sent via text This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply Service map data © OpenStreetMap contributors When Jeffrey Nivala was a graduate student his work station was a vibration-dampening table covered in electronic instruments: a patch clamp amplifier All those trappings were trained on one tiny focal point: a tube roughly a nanometer in diameter and the minuscule trickle of electricity that flowed through it chained to one of these things and trying to squeeze data out of it,” Nivala said was formed by a ring of proteins tunneling through a lipid membrane hoping for a group of seven proteins to self-assemble and poke through the lipid membrane waiting for minute fluctuations that would indicate that a macromolecule was passing through the pore a pocket-sized device that plugs into a laptop’s USB drive can deliver comparable information — from hundreds of pores at once “A student in my lab now can collect more data in an afternoon than I ever did in my Ph.D.” the biophysicists and engineers who developed the DNA sequencing technology are turning their attention to proteins which are substantially more difficult to analyze with a nanopore Researchers around the world are working to surmount the problems with proteins hoping to make single-molecule proteomics a reality The engine that powers nanopore sequencing is a pair of liquid compartments separated by a membrane with a positive charge on one side and a negative charge on the other When a protein nanopore inserts into the membrane it opens a path for ions to flow from one side to the other responding to the difference in charge between them (This difference in charge is measured in voltage; the rate of ion flow in response to a voltage difference is measured in current.) Most of the ions that make up the current are charged single atoms such as chloride and potassium Larger molecules also can feel an electrical pull The negative charge of DNA’s phosphate backbone will draw it toward a positive charge just as the difference in charge pulls the chloride ions But once that strand of DNA enters the pore allowing relatively few smaller ions to squeeze past and cross the membrane That reduction in the movement of charged particles is reflected in a change in current picked up by electrodes on opposite sides of the membrane Each of the four DNA bases has a different chemical identity and blocks ions to a different degree Like four different people casting distinct shadows as they pass through a door from a bright room into a dark one the nucleotides can be identified by their signature current changes Left to its own devices, a linear strand of DNA would zip through the pore rapidly, although not quite as fast as the ions around it. Aleksei Aksimentiev a biophysicst at the University of Illinois at Urbana–Champaign said that slowing DNA down was key to the development of nanopore sequencing you just don’t have enough ions … so you might make a huge error when you determine the average value of the current.” To measure the change in current with more confidence researchers borrowed motors such as polymerases and helicases from cells’ DNA replication machinery; these enzymes bind to the DNA and advance just one nucleotide at a time In addition to finding ways to make each fluctuation in current last a little longer researchers also have developed algorithms to interpret the changing current and determine in real time which nucleotides are passing through the pore The technology can collect long sequence reads from a single molecule of DNA letting researchers draw conclusions that other technologies don’t offer Aksimentiev is one of the many researchers who hope to apply the same principles to achieve protein sequencing His lab has been working on the problem since transitioning from DNA sequencing in 2016 the work might lead to a portable device that could be used to determine the amino acid sequences and post-translational modification patterns of much longer pieces of single proteins than currently are possible using mass spectrometry which would allow researchers to study signaling and isoform expression in greater detail than ever “There are lots of challenges with proteins,” Aksimentiev said they’re not uniformly charged; they’re not linear most of the time they’re folded; and there are 20 amino acids plus a zoo of post-translational modifications.”   While the backbone of DNA carries a negative charge a polypeptide backbone is neutrally charged at physiological pH and its amino acid side chains come in a rainbow of sizes and polarities whereas DNA reliably carries a negative charge  All that heterogeneity doesn’t just make it difficult to move a protein through the pore; it also may make for a more complicated analysis Each of the four DNA bases produces a signature change in current and it is much easier to differentiate among four signals than 20 — or counting the possibility of post-translational modifications Researchers have begun to show that at least a few distinct amino acid signatures exist, but they have not yet resolved all 20. In the journal Nature Biotechnology in early 2020 researchers in Abdelghani Oukhaled’s lab in Cergy collaborating with Aksimentiev’s lab reported that they could differentiate between the nanopore current signals from short peptides that varied by just a single amino acid with seven identical negatively charged residues and one that varied The team found a predictable interaction between the size and charge of that single variable residue and the way the current changed as the peptides passed through the pore When they tried to measure a mixture of all 20 peptides the researchers found that they could pick out a few of the peptides with high confidence coaxing the peptides to linger longer in the pore would enable them to identify even more with confidence To make larger proteins pass through the pore in an orderly way smoothing away secondary and tertiary structures without damaging the structure of the proteins that make up the pore or the integrity of the lipid bilayer it is embedded in tested and optimized that he thinks “unfolding the protein and feeding it through is probably not going to be the critical part” of achieving protein sequencing As a grad student at the vibration table, Nivala who is now a research assistant professor at the University of Washington focused on the problem of turning a folded protein into a linear molecule that fits neatly into a pore was focused on showing the first proof of principle of using these motors to analyze these full-length intact protein strands with a nanopore,” Nivala said the writing was on the wall that the big challenges in nanopore-based DNA sequencing had been or were about to be solved.” Working in the lab of University of California, Santa Cruz, biophysicist Mark Akeson, Nivala developed a way to use unfoldases — enzymes that in their typical context in the mitochondria unfold proteins in preparation for proteolysis — to pull proteins through an alpha-hemolysin nanopore does to a structured protein what a determined tug on a stray thread does to a sweater; it applies force to a loose end that overcomes the stabilizing power of internal interactions within the protein Nivala developed a C terminal protein tag that combined a highly charged stretch of amino acids and a protein motif recognized by ClpX a voltage difference would attract the end of the protein through the pore just far enough to encounter ClpX stationed on the opposite side the enzyme “would grab on to the tail and start pulling.” tugging along the peptide backbone with enough force to unravel the structure on the other side of the membrane and pull the protein through While this solved the problem of moving a noncharged protein through the pore the pore that Nivala used was long and narrow; the portion of its tube that was the tightest squeeze had space for as many as 30 amino acids at a time Each of them could contribute to the overall flow of ions Instead of trying to identify a single silhouette against a lighted door imagine working with a shadow formed by a line of dozens of figures “It ends up being a very intractable problem to deconvolve that into amino acid sequences.” Scientists had solved a similar challenge in DNA sequencing but it was less complex because nucleotides are larger in size and smaller in number They also had assistance from the stepwise work of the enzyme motors which changed the signal by exactly one base every time Although ClpX pulled its target proteins in regular steps of about a nanometer one nanometer of protein backbone can include anywhere from five to eight amino acids Nivala detected changes in current that showed some features of the test protein he was using but he was unable to translate those changes back into a sequence of amino acids Cees Dekker a physicist at Delft University of Technology called the work “a very elegant idea … that faces some technical challenges.” which combines a proteasome with a nanopore uses motors from the proteasome component to drive an analyte protein into the pore which previously had been used for DNA sequencing Conjugating a strand of DNA to a negatively charged short peptide they applied voltage to make the conjugated molecule translocate through the pore Then they added DNA helicase that would pull in the opposite direction dragging the protein–DNA conjugate backward The helicase’s steps in one direction and the pull of electricity in the other added up to a seesaw that put tension on the linear molecule That gave the electrode measuring current enough time to get a reliable reading at each step The Dekker lab’s work also illuminated the importance of molecular modeling to understand what really happens inside a pore during a protein sequencing run While testing whether the DNA conjugation system could help recognize a peptide that varied by just a single amino acid Dekker’s team observed that the peptide with the largest variable amino acid they tried That’s the intuition we built from,” Dekker explained its volume goes up and (the current) goes down But this saw more of a wiggling signal.” Tryptophan is bulky; it makes sense that wedging it into a small pore would block most ions from going past The team expected that it would produce a drop in current which they thought would recover to the baseline The current they observed instead dropped dramatically but then went up indicating that the pore was becoming more open When the structure of a pore is known at sufficient resolution researchers can use supercomputers to run complex high-precision simulations of its behavior Aksimentiev’s lab specializes in this work peptide: everything is there,” Aksimentiev said “And once we have this truly atomic-scale representation of the experimental system … just like an experiment we can see ions moving through the pore and measure the current.” the team can peer into the pore moment by moment to find out why the current is changing as it is Aksimentiev’s group found that tryptophan formed a hydrophobic interaction with the amino acids in the inner wall of the pore “The intuitive argument that if you have something bigger in the pore “It’s just that once the tryptophan has passed … it sticks to the wall and brings everything to the side actually making more space for the ions to pass.” This peculiarity gave tryptophan a highly recognizable signature “One can see how one can potentially engineer a pore to have those specific interactions and look for difficult-to-distinguish stretches.” Would this phenomenon happen when any peptide containing tryptophan crossed through this pore Aksimentiev thinks it will depend on the peptide sequence and local chemical environment the finding illuminates the complexity of factors affecting the current readout Long said that researchers are accustomed to thinking about current as only a function of volume: In other words an amino acid’s size is the most important determinant of how ions flow past that’s probably an oversimplification Every individual amino acid has the potential to interact with the pore but also may increase the complexity of protein-sequencing currents dramatically there are many different pores to choose from “A polymer is like the notes of music,” Long said “The nanopore is like a musical instrument The same analyte might evoke different currents from different pores just as a melody sounds different when played on an accordion or a music box Long began to analyze protein structures using a nanopore which at the time was the only pore in use for DNA sequencing the lab next door at the University of Saskatchewan had a convenient plasmid on hand; the PI had trained in the lab that discovered a bacterial channel toxin called aerolysin and shared it with them Hemolysin and aerolysin are just two examples of a vast protein family (see sidebar: “Many pores Multiple labs have published on pores borrowed from various other bacteria: hemolysin These proteins have diverse characteristics Some are barely wide enough to squeeze a polymer through the central pore Others are shaped like funnels that narrow to one critical sensing region the complexity of how pores interact with proteins once inspired researchers in the field to try using solid-state nanopores built from carbon nanotubes or other fairly homogeneous nonbiological materials These nanostructures have advantages; they hold up in heat pH and detergent conditions that can linearize a protein but would destroy a biological nanopore and its lipid environment researchers hoped that engineered pores might yield a smoother interior surface minimizing the potential for interactions with biomolecules Nobody can make an atomic structure smooth.” can give designers more room to experiment we can make different structures we want.” “Nature has lots of different pores that it’s using for lots of different reasons,” Nivala said “We’ve probably barely tapped into what nature’s already built.” Meanwhile improved tools for protein design have led to researchers “starting to engineer protein nanopores from scratch that you can custom-tailor for different applications.” A spokesperson for the company Oxford Nanopore said that when working with a new bacterial pore the company makes and tests thousands of mutants interaction with DNA and signal clarity during sequencing declined to comment on protein sequencing for this article Nivala said that his lab works closely with the company He said the pore currently used in Oxford Nanopore’s DNA-sequencing flow cells But whatever drawbacks it may have are outweighed “If we are able to do anything useful some other lab could pretty much instantly then adopt that technology and that technique,” he said “They don’t need to be nanopore experts.” because Oxford’s technology is mature Nivala’s team can focus easily on collecting and analyzing data “We’re going to need lots and lots of data … to develop machine learning algorithms that can make sense of these signals and decode them back into amino acid sequences.” Researchers have developed software tools that reliably can identify a short test peptide from a list of options They still have a long way to go to achieve true protein sequencing Software that can decrypt the tiny fluctuations in current will be as important as choosing the proper molecular machinery The race is on to develop algorithms that can determine a sequence starting with no information about it the combination of single-molecule resolution with long reads could open a door to single-molecule studies of proteins that are currently impossible Researchers are interested in applications such as antibody sequencing and investigating how multiple post-translational modifications on a single protein nanopore protein sequencing systems can already be used for interesting applications such as scanning short peptides for negatively charged post-translational modifications And researchers are confident that more advances are just over the horizon “It’s all coming together,” Aksimentiev said “It’s a very exciting time for single-molecule protein sequencing.” The sequencing community regards pore-forming proteins as a useful biochemical tool Melanie Ohi, a structural biologist at the University of Michigan who studies the assembly of numerous protein complexes, keeps several boldly colored images of pore complexes tacked to the white board in her office. The largest depicts VacA, a pore-forming protein from the stomach pathogen Helicobacter pylori, whose structure her lab solved a few years ago “These toxins … are beautiful They are the prettiest molecules you’ll ever look at.” Ohi is interested in what she called toxins’ “fascinating ability to live in two very different environments.” secrete the subunits that make up protein pores into their environment meaning that the toxins must be water soluble those soluble subunits also must find one another assemble into that pleasing symmetrical ring and accomplish a biophysically tricky insertion into a membrane requiring that they show a hydrophobic face is helping researchers to understand the many conformations these proteins can adopt at progressive stages of this attack the pore that eventually forms can render a membrane permeable to ions or to larger molecules “A lot of bacteria have these types of toxins,” she said “They’re used either to create a niche for the bacteria to live and grow in; or as a way for bacteria to deliver effectors into a host cell; or as bacterial warfare.” a professor at Nanjing University who has been working on developing protein analysis methods that use nanopores since the early 2000s said that he does not expect pores to overtake mass spectrometry for proteomics completely There are uses for which mass spec has downsides, such as differentiating amino acids or modifications with identical mass They also work fast; a researcher with a nanopore can detect biological systems in real time “Mass spec is a fundamental technique for protein sequences Nanopore sequencing cannot be a replacement especially for some application environments.” both the single-molecule long reads obtained through nanopore sequencing and the massively parallel short reads obtained through next-generation sequencing have advantages and are useful for different types of study Long said some of the most exciting applications for nanopores open completely new capabilities he is interested in using nanopores to make enzymatic synthesis faster at an industrial scale Whereas chemical syntheses can use heat and stirring to increase the frequency with which reactants collide enzyme-catalyzed systems can’t stand up to heat and vigorous stirring what if researchers found ways to embed catalytic sites into the walls of a pore and then draw reactants in He also emphasized that while sensing specific amino acids is useful nanopores also can be used to sense other types of analytes Structural biologist Melanie Ohi said that many basic scientists who study bacterial pores are interested in the possibility of using them for drug delivery “A lot of these toxins are very cell-specific,” she explained Although it will take more research to determine whether their specificity comes from being released near those cells or from recognition of cell surface molecules “It seems like it could be very powerful if you could take the bacterial toxin that is delivered to a very specific cell and re-engineer it to deliver a drug.” Become a member to receive the print edition four times a year and the digital edition monthly Laurel Oldach is a former science writer for the ASBMB and we’ll send you a weekly email with recent articles Scientists find that liver protein inhibits of pertussis toxin offering a potential new treatment for bacterial respiratory disease Read more about this recent study from the Journal of Biological Chemistry Scientists discover that triacylglycerol synthesis enzyme drives lipoproteins secretion rather than lipid droplet storage Researchers analyze protein and RNA data across 13 cancer types to find similarities that could improve cancer staging Read about this recent article published in Molecular & Cellular Proteomics Scientists develop a software tool to categorize microbe species and antibiotic resistance markers to aid clinical and environmental research Scientists develop a bioinformatics program that maps omics data to metabolic pathways Read about this recent article published in Molecular &amp; Cellular Proteomics Learn how the JBC associate editor went from milking cows on a dairy farm to analyzing kinases in the lab the 5-foot-2 Nivala scored in bunches for the Spartans and returns to the T&G Super Team after collecting 105 points (76 goals she finished with 257 goals and 123 assists for a school-record 380 points Other school records include most goals (9) and points (13) in a game Oakmont only went 5-11 (4-8 Midland B) this season but Nivala enjoyed a 10-point game (8 goals then her six goals and seven assists accounted for her 13-point afternoon against Wachusett she also earned Wachusett C all-star status in soccer last fall Nivala is headed to Southern New Hampshire University and would like to coach Division 1 college lacrosse someday She is the daughter of Linda and Kevin Nivala of Westminster The dates displayed for an article provide information on when various publication milestones were reached at the journal that has published the article activities on preceding journals at which the article was previously under consideration are not shown (for instance submission All content on this site: Copyright © 2025 Elsevier B.V. Antti Nivala discusses his company’s origins the differences in various c-suite roles and the need for SaaS companies to show they can operate in a ‘cash-efficient manner’ Antti Nivala is the founder and CEO of M-Files an information management company that helps organisations easily manage their documents The company’s platform aims to give businesses a way to instantly find the information they need automate business processes and enhance information control The company has grown into a massive entity over the years offices in eight countries and more than 5,000 customers around the world Nivala said the inspiration of M-Files came from a problem his father encountered as the head of an architectural engineering firm “Legacy document management solutions underpinned by a single physical folder hierarchy were creating data silos across his firm which resulted in misplaced files and duplicate documents,” Nivala said I considered how I could create a solution that provided all employees with the information they required at any given time Nivala began working on the software in 2002 in Tampere Fast forward to 2023 and M-Files has managed to reach ‘Centaur’ status exceeded €100m in annual recurring revenue “Knowing that we have reached this level of success from such humble beginnings is an incredibly uplifting feeling,” Nivala said Nivala has held various positions within M-Files over the years being the development director in its early years before being CEO between 2005 and 2012 and shifting to CTO and chief product officer at different points When discussing the differences between these roles Nivala said that his time as a CTO involved a lot of “hands-on work” while being CEO is “far more culture-driven” but shortly after making the transition to CEO I realised that solving every issue as head of a company is impossible my purpose is to create an environment where employees feel empowered to take on individual tasks and really excel.” Nivala returned to the CEO position towards the end of 2019 and has stayed there to the present day In terms of creating the right environment he said he tries to engage and inspire his staff by speaking transparently and honestly to create a sense of unity “If I’m open with the wider team I find that this really helps people feel comfortable voicing any concerns they have or ideas they want to bring forward,” he said “When everyone has the same insight into the business they’re more likely to feel valued and understand that we’re all working towards the same goals.” But despite the various roles he has held within the company he claims to be a “techie at heart” writing software since he was 13 years old But it seems the different roles has taught him a lot over the years I’d tell myself that soft skills are just as important as technical proficiency when it comes to kickstarting a business,” Nivala said “I wish I’d known that time spent building relationships with customers and partners can be just as valuable as time spent developing a product.” The tech sector has experienced a crunch in recent years with many big tech companies laying off staff and seeking to tighten their costs Nivala said software-as-a-service companies have also experienced a “reset back to the realities of business” after experiencing a “growth-at-all-cost” period This change does not appear to have fazed him “SaaS companies now need to show that they can operate in a profitable and cash-efficient manner because we have always ensured that what we do delivers real business value to our customers.” Nivala said M-Files is looking to the benefits AI can bring to the document management sector describing AI technology as the “flavour of the month across practically every industry” “We’re leveraging the capabilities of generative AI to make sure the M-Files platform is as advanced and intuitive as possible,” Nivala said “Our platform uses AI to update metadata assign permissions and intelligently act on content.” but Nivala said the company took risks in its earlier days The company was bootstrapped in its early years in Europe and established a US presence before getting any outside investor money which Nivala looks back on as a “bold and even risky move Nivala took a new leap himself in recent years leaving his home country of Finland in 2019 with his family to live in the US “It’s been a big change for the family and I hope they will see the value in it in the long term.” Nivala said he is a “keen runner” and that he tries to complete one big marathon each year competing in the New York City marathon over the past couple of years as part of the New York Road Runners (NYRR) Team for Kids to raise funds for the charity Inspiration and words to live by can be found anywhere and Nivala said the book Good to Great by Jim Collins is a book that had a “very significant impact” on his approach to leadership One of the key quotes he remembers from the book is to “Mix personal humility with professional will” “I think this perfectly encapsulates the mindset of a great leader,” Nivala said “It’s vital to remember that we’re all human and the people around you will thrive if you treat them with compassion and respect “Many of the ideas the book explores have laid the foundation for success throughout the M-Files journey.” 10 things you need to know direct to your inbox every weekday. Sign up for the Daily Brief Silicon Republic’s digest of essential sci-tech news editorial@siliconrepublic.com Here’s how AI and ML will drive smarter information management Current processes too often prevent knowledge workers from finding critical information But artificial intelligence (AI) and machine learning (ML) advancements in areas ranging from natural language processing to cognitive search will help reveal insights in data that can improve business shares Antti Nivala But companies are often so awash in information that they have difficulty making the best use of it Many businesses still rely on manual processes to search for introduce errors or fail to meet compliance requirements Valuable information can get lost in translation in speech-to-text apps or in the mix of multiple languages used within international organizations Process bottlenecks can unexpectedly slow down business Information can also be siloed in separate systems blocking access to those who could use it.  These problems keep critical information out of the hands of employees and prevent businesses from gaining insights from their data More efficient information management can help them better understand their customers and markets thereby delivering better products and services.  recent advancements in AI and ML are changing the game in information management including more comprehensively indexing files (including audio and video files) improving speech-to-text and language translation processing more accurate searches for key insights and information Al and ML enable businesses to not just manage information but to find meaning within it employees spend less time fruitlessly searching for vital information and more time making use of it to improve the business See More: Five Reasons to Use Generative AI to Automate Building Designs Here are five ways that AI and ML will change information management for the better: such as AI’s improvement in understanding information context will enable machine translation to achieve near-human capabilities this year Human translators reviewing a machine’s translations will likely find fewer mistakes—perhaps no more than they would find in content translated by a human The automated translation of large content archives will increase organizations’ ability to share data by making previously “hidden” content searchable and discoverable This will enable multinational and multilingual enterprises to give employees access to all its content no matter with which language the original content was written Speech-to-text apps have always come in handy but they’ve also come with a few quirks that transcribed some text inaccurately With AI and ML models being continuously trained and improved the gap between the spoken word and text transcriptions is closing These advancements will likely continue forward allowing speech-to-text applications to move closer to 100% accuracy and enabling companies to improve knowledge sharing by making content more searchable and accessible.  Advances in natural language processing (NLP) allow AI-based solutions to process language in a way similar to how people’s brains work NLP can extract meaning from the speech in audio files giving it the capacity to understand a user’s intent This allows systems to apply metadata tags to audio and video files indexing those files in a way once reserved for text documents It will enhance how knowledge workers search for information and allow information management systems to better deliver relevant information to end users Knowledge workers spend an inordinate amount of time not finding the information they’re looking for. Several studies in recent years have estimated that workers wasteOpens a new window anywhere from 30% to 50% of their time searching for the information they need to complete their tasks.  advancements in cognitive search capabilities will enable knowledge workers to spend less time searching for the information they need and more time using it while focusing on what drives the bottom line Enterprises will be able to go beyond simple keyword searches instead accessing content based on context due to advanced indexing capabilities—including advancements in metadata tagging.  By combining information with a domain-specific or organization-specific knowledge graph enterprises will allow employees to easily find content powered by context without spending valuable time going through endless search cycles.  Employees today are drowning in information and most traditional summarization technologies don’t always offer much relief They use an “extractive” approach selecting a subset of relevant sentences from a document to form a summary But those sentences don’t always conform with one another limiting the summary’s ability to fully grasp the content.  Large language models (LLMs) have seen a huge increase in popularity recently with OpenAI’s ChatGPT LLM making headlines People are realizing the business value of this technology and the continued advancements of it are enabling “abstractive” summaries to be automatically generated delivering a more human-like and effective result. LLMs can use abstractive summarization to reorganize language in the text abstractive summarization can give employees more precise information more quickly shortening the time they spend searching for it and improving their productivity AI and ML are adding significant value to the massive amounts of data that enterprises collect every day Recent advancements are making information easier to find and comprehend empowering organizations with insights that were previously very difficult to uncover Further advancements in AI and ML are transforming information management into a key business facilitator It’s a trend that will certainly continue well into the future but one that is also already happening right now by empowering innovative businesses and organizations to gain real insights from their data Installing Microsoft Office 365 on an iPad Pro Emotional intelligence When was the first time you felt a disturbance in the Force? Toolbox will become Spiceworks News & Insights ambassador of Finland to the United States (center) president and CEO of the Austin Chamber of Commerce (center right) for a ribbon cutting ceremony in celebration of M-Files' new Austin office and North American headquarters Document management platform M-Files celebrated the grand opening of its new North American headquarters at 500 W The Finland-based company's headquarters was previously located in Plano The M-Files ribbon-cutting ceremony was attended by the Austin Chamber of Commerce and a delegation from the Finland Ministry for Foreign Affairs Glorie joined Community Impact Newspaper in January 2021 after graduating with a degree in journalism from The University of Texas at Austin she was an intern at KUT Public Media in Austin Thirty-three riders (and 25 horses) came out June 5 for the Martha’s Vineyard Horse Council’s Spring Fling horse show at the Agricultural Hall fairgrounds Classes included leadline division (ages two and up) Riders showed in Western and English tack and came from a spectrum of equestrian focuses Horses ranging from Clydesdales to Hackney ponies filled the fairgrounds Leadline rider Zoe O’Sullivan received the Braveheart Award for courage and the Sportsmanship Award went to Ashley Casey whose horse was not feeling well enough to come to the show The Horse Council thanked event sponsors Martha’s Vineyard Savings Bank Vineyard Equestrian and Pete and Suzanne Sprayregen as well as all volunteers and coordinators and judge Tobi Stall Mallinson The event was spearheaded by the Hunter Show Committee consisting of riders and parents of riders: Lisa Nivala The next horse council event will be a Dressage Show on July 23. For more information visit mvhorsecouncil.com Leadline Pleasure First Place Tie for Participation (all of the above) Subscribe or become a Friend of the Vineyard Gazette and receive our free newsletters and free and discounted tickets to Gazette events along with our award-winning news and photography Metrics details a technique to unfold proteins for processive translocation has yet to be demonstrated Here we describe controlled unfolding and translocation of proteins through the α-hemolysin (α-HL) pore using the AAA+ unfoldase ClpX Sequence-dependent features of individual engineered proteins were detected during translocation These results demonstrate that molecular motors can reproducibly drive proteins through a model nanopore—a feature required for protein sequence analysis using this single-molecule technology Prices may be subject to local taxes which are calculated during checkout Characterization of individual polymer molecules based on monomer-interface inter- action Automated forward and reverse ratcheting of DNA in a nanopore at 5-Å precision Reading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase Controlling a single protein in a nanopore through electrostatic traps Single-molecule protein unfolding in solid state nanopores mutant protein unfolding and phase transition detected by single-nanopore recording an ATP-powered unfolding and protein-degradation machine Single-molecule protein unfolding and translocation by an ATP-fueled proteolytic machine A protein-conducting channel in the endoplasmic reticulum The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer Solution structure of a yeast ubiquitin-like protein Smt3: the role of structurally less defined sequences in protein-protein recognitions The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system Dynamics of substrate denaturation and translocation by the ClpXP degradation machine topology and orientation of the electric field on the interaction of peptides with the α-hemolysin pore Interrogating single proteins through nanopores: challenges and opportunities Unfolding of proteins and long transient conformations detected by single nanopore recording Rebuilt AAA+ motors reveal operating principles for ATP-fuelled machines Download references The authors thank Oxford Nanopore Technologies (Oxford Martin (UC Berkeley) for supplying ClpX-related expression plasmids and for helpful discussions on their use Olsen commented on drafts of the manuscript This work was supported by a UC startup grant to M.A. and by equipment purchased previously using National Human Genome Research Institute grant R01HG006321 The ClpX-ΔN3 BLR expression strain was obtained from A as was a his-tagged ClpP expression strain performed the protein engineering and production conceived and performed experiments and co-wrote the manuscript designed the nanopore platform and directed the project is a consultant to Oxford Nanopore Technologies Download citation Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology Chemical Engineering JournalCitation Excerpt :Removal mechanisms could promote pollutant removal from the wastewater that is produced during the sludge dewatering process [8] raw sludge produced in a treatment plant [1] and anaerobic sludge [7] were dosed in treatment wetlands to purify drained wastewater Organic and nutrient concentration of the drained wastewater was comparatively higher in many of such reported studies that might not fulfill effluent discharge guidelines in many countries we inferred that the indirect effects of plant played an important role in MC-LR removal plants can release oxygen to the rhizosphere to stimulate the degradation of organic matter by aerobic microorganisms (Hu et al. the growth of rhizomes can enhance the retention and absorption of organic matter which can benefit the assimilation and mineralization of organic matter by plants and microorganisms (Hu et al. of Worcester passed away peacefully Wednesday She is survived by her devoted brothers and sisters; Anthony A "Pam" Gullbrand and her husband Donald of Worcester; Caledonia and her husband Richard of Franklin Alfego and his wife Maryann of Shrewsbury; nieces Mary Ann was a daughter of the late Anthony A She graduated from Worcester Girls Trade and has lived here all her life school department over 19 years before retiring in 2002 who enjoyed sharing time with and around her family and friends A period of visitation will be held from 9:30 a.m will build the largest battery energy storage project in the Nordics The project will add 70 MW/140 MWh of storage capacity to SEB Nordic Energy’s Finnish portfolio which already includes wind and hydropower Located in Nivala Municipality in Finland’s Ostrobothnia region the project is expected to be completed in 2026 The Nivala battery storage project marks SEB Nordic Energy’s second significant investment in Finland in a short time It follows a 125 GWh wind portfolio comprising 13 wind turbines across three wind parks This investment further strengthens the fund’s existing portfolio of renewable energy assets in Finland and improves the portfolio’s system flexibility and balancing capabilities creating new opportunities for optimising energy output.  developing a pan-Nordic IPP* for renewable energy The battery system in Nivala further diversifies the fund’s Finnish portfolio and accelerates the renewable energy transition—aligned with the fund’s mission to drive sustainable energy solutions,” says Richard Gavel Ingrid Capacity develop and optimise grid-scale battery energy storage and flexible assets across Europe SEB Nordic Energy formed a strategic partnership with Ingrid Capacity in September 2024 SEB fund invests in a large Swedish battery storage system The fund is intended for professional investors only and the fund can decrease in value or increase significantly Prospectus and sustainability-related disclosures are available from SEB SEB Asset Management AB has prepared this material BrandRukkaWINTER'S coming and I’m fed up with second-best – I spend too much of my life on a bike So I’ve got this.  It’s a Gore-Tex suit from Rukka using it on my 60-mile motorway/town commute into London And what I can tell you is that it has made a good one I’ve got an old suit with a Gore-Tex removable liner the outer shell is a three-layer Gore-Tex Pro laminate But it’s got abrasion-resistant panels in the right places and flexible CE-approved armour inserts which are large enough to curve right around shoulder which is held in place by a zip at either side It’s got a YKK waterproof main zip and seems well designed everywhere with outer cuffs that easily go over the gauntlets of your gloves which help keeps draughts out (my old ones don’t and I was forever pulling them up) and of course they zip to the jacket One of the best features is the thermal lining Neither attach to the outer – you just put them on separately With my last suit I was always undoing buttons and zips to take the thermal liner out when it warmed up a bit You can wear the upper part on its own as down jacket I suppose you could wear the trouser liner separately too I think it’s definitely the warmest motorcycle suit I’ve owned I’d normally have the heated vest out by now When not in use both parts of the thermal lining compact neatly into stowaway bags I suppose one thing it lacks over my previous suit is the ability to remove the Gore-Tex lining altogether to stay cool on really warm days I think it’s probably more suited to northern Europe than southern It seems by quite a margin the best riding gear I’ve owned The price is high – the jacket is £1,199 and the trousers another £7,99 – but so is the quality Details exclusives and promotions direct to your inbox For more information, read our Privacy Policy © Crash Media Group Ltd 2025.The total or partial reproduction of text photographs or illustrations is not permitted in any form Regardless of how COVID-19 finally plays out the changes brought in response to the pandemic The COVID-19 pandemic has dramatically impacted how organizations operate from heavy reliance on remote work and online interactions to changes in business strategies Data has become the lifeblood of practically every enterprise in the hybrid reality at the core of those responses shares key takeaways and learning points for data managers it’s a good time to look at the lessons it has taught us about data and what organizations need to do to ensure its quality and reliability Here are the key takeaways about data from the pandemic that organizations should be sure to carry forward See More: To Build Customer Trust, Prioritize Data Protection and Personalization One of the biggest takeaways from the pandemic is the critical importance of having a solid which for most organizations means a cloud-based solution Public health organizations felt the impact immediately. Traffic to the Centers for Disease Control and Prevention’s website, as you might expect, exploded in 2020Opens a new window recording more than a billion page views in March that year alone compared with just under 100 million during March 2019 And traffic for 2021 was still three times greater than in 2019 with COVID-related traffic accounting for 56% of the CDC’s total page views.  trustworthy data during the most severe spikes in traffic underscores the need for infrastructures with high availability and easy failover which can offer nearly limitless scalability and the ability to adjust to sudden spikes in traffic within minutes It’s always been important never to take the quality of your data for granted but that became especially true during the pandemic Organizations that perform emergency response distribute medical data or help with recovery efforts saw that importance up close but every organization needs to trust its data if it is to be effective organizations need to know where and how often it’s being collected An information management solution can automatically add metadata to information to give it this critical context providing an organization confidence in all of its data.  Data without context is just random information the internet was rife with posts comparing the infection rates of different countries—the United States and Italy for instance—in terms of raw numbers without context such as the age of the population the availability of health care or for how long the disease had been spreading the numbers didn’t have any real value at all Data context is essential and goes far beyond simple dashboard visualizations It’s also important to know what’s behind the data a business proposal might rely primarily on existing agreements and recent correspondence but past agreements or correspondence related to the proposal Context cannot only add relevant information but also filter out extraneous unimportant noise that might otherwise influence a decision an information management platform that uses advanced analytics powered by artificial intelligence can quickly locate relevant information to put data in clear context whether the issue at hand involves contracts Data-driven decisions can’t be effective without context See More: How Brands Can Keep Pace With Third-Party Data Changes businesses and other organizations run on their data developing a truly data-driven culture has proved to be an elusive goal A data-oriented culture involves mutual trust and data-centric processes focused on collaboration and the quality and security of data.  An organization with such a culture already has the components it needs to run smoothly there are concrete steps they can take to create a data-oriented culture integrity and security of an organization’s data as well as controlling access to data.  A data-oriented organization will make data a part of all decisions up-to-date and available to everyone who needs it It can help organizations respond quickly to crises as they arise and handle any new data challenges that come with them data-based processes was already underway in many organizations before the appearance of COVID-19 but the pandemic accelerated those transformations and organizations need to implement information management practices that ensure their data’s availability Create your free account to receive personalised content alerts and Re:action our weekly newsletter of the top chemical science stories handpicked from a range of magazines journals and websites alongside insight and analysis from our expert editorial team Tell us what you think. Take part in our reader survey By 2024-09-23T10:00:00+01:00 which uses a biological motor to pull a protein through a tiny nanopore The researchers at the University of Washington have also achieved a significant breakthrough in detecting post-translational modifications to full-length protein structures at single molecule resolution The nanopore sequencing technique relies on a bacterial protein (purple) to draw the unfolded polypeptide chain through the pore (pink) yet researchers have identified 1 million different protein structures This diversity arises from genetic mutations and post-translational modifications – such as the addition of chemical groups or carbohydrate chains – that alter protein functions These modifications play a key role in regulating complex biological processes and can also influence disease development Mapping this diversity could greatly enhance our understanding of cellular functions and extend opportunities for more specific disease interventions but traditional methods struggle to deal with the three-dimensional structure of proteins To overcome this, Jeff Nivala’s lab in collaboration with Oxford Nanopore Technologies has developed a system for full-length protein sequencing using nanopore-sensing technology ‘This innovative technique enables researchers to read long offering new possibilities for understanding complex biological processes and diseases’ works by drawing a long biopolymer through a biological channel – a nanopore – using an electric field Structural variations are identified by measuring changes in the electric current applied across the nanopore As different chemical groups cause different disruptions this gives them a unique chemical signature that is used to decode the polymer sequence Before a protein can be passed through a nanopore for sequencing its secondary and tertiary structures must be unfolded Nivala’s team first modified a protein of interest by attaching a negatively charged ‘tail’ to draw the protein towards the pore when an electric current is applied They also added a bulky ‘stopper’ sequence which prevents the protein from passing completely through the channel The stopper has a binding site for the enzyme ClpX ‘which helps “unfold” and pull the protein through the nanopore’ ‘ClpX has a unique stepping mechanism that allows it to move proteins in approximately two-amino-acid steps.’ The amplitude of the electrical current across the channel varies as the protein is pulled through the nanopore – a consequence of the amino acids present These changes in amplitude can then be used to determine the protein’s sequence Changes in charge as the polypeptide is drawn through the nanopore can be detected and used to identify which amino acids have passed through It can also detect if an amino acid has had any changes made to it as it loses its grip on certain stretches of amino acids Nivala’s team made use of this apparent imperfection to strategically insert ‘slipping sequences’ into the protein chains the researchers were attempting to sequence ‘We could control the speed and direction of the protein’s movement allowing us to reread sections and improve accuracy,’ says Nivala ‘The ability to control this motion allowed us to develop a more detailed picture of the protein’s sequence.’ Despite its promise, the need to attach ‘tail’ sequences to the proteins and the added complexity of interpreting current measurements for unknown sequences poses significant challenges for high-throughput protein sequencing. ‘The challenge is to make something that can be used by anyone rapidly and easily without requiring them to have some kind of high-level expertise,’ says Yujia Qing K Motone et al, Nature, 2024, DOI: 10.1038/s41586-024-07935-7  Site powered by Webvision Cloud The Vineyarder girls tennis team faced a unique challenge in its match against Sandwich High School Wednesday afternoon at the Ned Fennessy courts in Oak Bluffs Both teams came into the contest unbeaten at 4-0 unable to field a full boys team this season came to the Island with three boys playing on the girls team it was business as usual for the Vineyarders 1 singles player Hunter Tomkins got MV off and running with a 6-1 2 singles sophomore Cali Giglio put the Purple up 2-0 overcoming an early 0-2 deficit in the first set to beat eighth grader Andrew Emeu 6-2 The Vineyarders clinched the best-of-five series after second doubles Paige Anderson and Evelyn Brewer defeated Yona Forant and Grace Ruthera 6-0 First doubles Tessa Hammond and Andrea Morse made it 4-for-4 after topping Emily Lopes and Kaitlin Landry 6-1 3 singles between Vineyard junior Karrine Nivala and Sandwich sophomore Pablo Gonzalez focusing on quality tennis is the only thing that matters the singles matches and the doubles matches,” he said “The girls who were up against the boys did a good job in just focusing on tennis That comes with their training and what they’ve been taught on how to play tennis it’s not going to say “boy” or “girl,” it’s usually like “Wilson,” “Dunlop,” or “Penn” on it or something like that.’ It’s all about tennis People who gain the best results are the ones who just focus on the ball and their strokes and their development The Vineyarders are back on the home courts versus Barnstable Journal of Cleaner ProductionCitation Excerpt :The hybrid systems are usually the more complex CWs design system with significant functional improvement but higher capital budget (2019a,b) while treating domestic wastewater through comparison of the conventional and intensified SSF-CWs reported that pollutants removal rates increase with design complexity in the order; HF<VF<intensified It has been established that high DO which characterizes a typical VF-CW system significantly suppresses the N-reduction process (denitrification) which results in a high concentration of nitrate-nitrogen (NO3−-N) relative to ammonium-nitrogen (NH4+-N) beyond the macrophytes demand whereas the HSSF-CWs is noted for the low oxygen diffusion capacity to the atmosphere Ecological EngineeringCitation Excerpt :Nevertheless earlier literature reported that most of the tidal flow CWs were applied in lab-scale and the treatment affected by various factors Effluent recirculation has been applied in CWs for multi-purpose including in the case of cold climate CWs to maintaining increased hydraulic dynamics and rising water temperature and the recirculation ratio varies from 0.5 to 3.0 in pilot and/or full scale (Avila et al. (2009) run two filtration systems in Estonia in the winter between the temperature of −7.5 and − 35 °C to verify the effects of effluent recirculation on the treatment efficiency Having clinched Cape and Islands League titles on May 15 the Martha’s Vineyard Regional High School (MVRHS) girls and boys tennis teams improved to 14-0 overall and 13-0 in league play with wins over Sturgis East on Tuesday winner over Brook Meyer; Hannah Rabasca moved up to second singles win over Liz White; and freshman Karinne Nivala won at third singles against Grace Rizzuto First doubles Molly Pogue and Chesca Potter prevailed 6-1 and the second doubles duo of Alex Rego and Tessa Hammond defeated Keshet Baumflek and Sophie Lemmon Chris Ferry bested Will Furtado at first singles 6-0 Spencer Pogue left his usual first doubles spot for second singles and defeated Noah Whelpley 6-0 and third singles regular Eric Reubens played first doubles with Owen Favreau in a 6-1 Second doubles Luke Nivala and Ben Belisle closed out the win with a hard-fought 6-2 10-4 (tiebreaker) win over Evan Halgopen and Hunter Proft Sturgis East scored their only point by default in the third singles match Top-seeded first singles Kelly Klaren won all three of her matches on Saturday and Sunday at Old Rochester Regional High School in Mattapoisett to advance to the final round of the MIAA Girls South Individual Tennis Tournament Klaren defeated Lindsay MacDonald (Monomoy) 6-0 win over Alessia Cahoon (Ursuline) in the quarterfinals on Sunday gaining a berth in the South Sectional finals She will face Alex Prudente (Notre Dame Academy Hingham) on Saturday at 10 am at Old Rochester In the Boys South Individual tourney at Barnstable High School on Saturday 2 seed Chris Ferry defeated Vineyard teammate Eric Reubens 6-0 then defeated Evan Abramson of Sandwich 7-5 1 Harry Collomb in the semifinals on May 19 To appreciate Louie's Trophy House Grill you really need to step inside its doors and see the heads of white buffalo and Watusi cattle stuffed and mounted on the walls You need to meet the customers who have sat on its stools for 60 years You need to sink your teeth in to a legendary Garbage Burger It's a place of domestic beers and $1 tequila shots But it's also a showcase for owner Mike VanDemaele hunting trophies which range from bears to white buffalo to deer There's also a stuffed squirrel's hindquarters which one assumes was not taken during a big game hunt Norm): David Nivala said it's the range of people who make Louie's great "It's all socio-economic classes Everybody comes in here at one time or another during the week There are the softball teams and the work class but everything is fresh." James "Sarge" Dumont first sat down at Louie's on Sept People are attracted to you and it's a like home," Dumont said he was sporting a pair of suspenders emblazoned with the word "Louie's" — a gift from the staff The Must Try: There are no signature cocktails here It's the home of the $1 tequila shot and a place to order yourself a Pabst Blue Ribbon then the shot ski might be in order – a ski with four double shot glasses Everyone raves about the pizza and there are plenty of fans of the deep-fried pickles and then there is that Garbage Burger— a burger topped with olive sauce tomato and pickle served on grilled Texas toast The Inside Scoop: General manager Rachel Miller said Linda S. Mah is a reporter for the Kalamazoo Gazette. Reach her at 269-303-6567 or her at lmah@mlive.com. 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All rights reserved (About Us) The material on this site may not be reproduced except with the prior written permission of Advance Local Community Rules apply to all content you upload or otherwise submit to this site YouTube's privacy policy is available here and YouTube's terms of service is available here Ad Choices \n m_gallery = \"mlive_visits_kalamazoos_louies\";\n m_gallery_id = \"15844691\";\n m_gallery_title = \"MLive visits Kalamazoo\\'s Louie\\'s Trophy House Grill in search for Michigan\\'s Best Neighborhood Bar\";\n m_gallery_blog_id = \"4472\";\n m_gallery_creation_date = \"Sunday 1:51 AM\";\n m_gallery_permalink = \"http://photos.mlive.com/4472/gallery/mlive_visits_kalamazoos_louies/index.html\";\n m_gallery_json = \"https://blog.mlive.com/photogallery/4472/15844691.json\";\n m_gallery_pagetype = \"embed\";\n m_gallery_type = \"photo\";\n <\/script>\n Gallery: MLive visits Kalamazoo's Louie's Trophy House Grill in search for Michigan's Best Neighborhood Bar The Martha’s Vineyard Regional High School (MVRHS) girls and boys varsity tennis teams dominated their opponents 5-0 during their first playoffs matches The girls faced Pope Francis Preparatory School on Saturday and the boys took on Northampton High School on Tuesday at Ned Fennessy Courts in Oak Bluffs The girls’ victory follows a strong 17-1 regular season We had the experience on our opponents,” MVRHS girls tennis head Coach Bill Rigali said The boys’ win also follows a strong regular season with a record of 15-2 “We did well,” MVRHS boys tennis head Coach Jonathan Bates said MVRHS’ Hunter Tompkins had “the most competitive match” against Pope Francis’ Katelyn Horky MVRHS’ Calie Giglio and Karinne Nivala “controlled their matches fairly easily,” beating Paige Cook and Emma Cassell respectively with scores of 6-0 MVRHS’ Jacob Riis beat Northampton’s Adam Metz 6-0 The overwhelming MVRHS wins took place during the doubles matches as well MVRHS’ Tessa Hammond and Evelyn Brewer beat Pope Francis players Olivia Puppulo and Keira Hearn with scores of 6-0 MVRHS’ Alana Nevin and Sofia Balsas-Fuentes also took down Pope Francis’ Jennie Budrecki and Emma Corridew with scores of 6-0 MVRHS’ Teagan Myers and Caleb Dubin beat Max Cahillane and Durell Patrick 6-0 MVRHS’ Kert Kleeman and Kyle Levy also beat Maitreya Abend and Ken Armstrong 6-0 After getting past Pope Francis in the first round of 32 MVRHS is preparing to face Old Rochester Regional High School in the round of 16 during the next playoff matches The matches were scheduled to take place at Ned Fennessy Courts in Oak Bluffs on Wednesday “We’re just getting ready to play some really good tennis matches,” Rigali said The boys will be preparing to take on their next opponent at Ned Fennessy Courts in Oak Bluffs on Friday Paul Karasik walked into the West Tisbury Free Public Library late last Saturday afternoon looking for a gong At the same time library director Beth Kramer was busy helping someone fill out a passport application Lisa Nivala and her daughter Karinne were looking for their favorite book in the children’s section And Insley Julier was taking her time in the Vineyard Collection area of the library acquainting herself with the nuances of the Island Karasik’s quest for a gong for that evenings’s Martha’s Vineyard Charter School annual rock auction it felt like a normal fall day at the library save for the cardboard boxes lining the aisles of books and the staff quietly packing away the last of the library’s collection The current iteration of the West Tisbury library closed its doors on Saturday to make way for a new and larger facility Ground-breaking is expected next month for the new building and the library will be temporarily housed at the Middletown Village on State Road in North Tisbury across the street from Conroy’s Apothecary the final hour of the library was quiet and business continued as usual Many of the books had already been taken off the shelves and either delivered to the temporary location stored away or sent off to patrons’ homes for safekeeping and lending Knitting expert Kathy Minkiewicz is the keeper of the knitting books and town poet laureate Justen Ahren will be overseeing the poetry books but a year’s worth of Men’s Health magazines still lingered on the stacks Kramer reflected on the memories made in the old space and the difficulty of the coming transition “It’s hard going into something that’s so uniquely small,” she said of the temporary space “It will not be a browsing library.” Flexibility and resilience were the buzzwords to describe the coming days weeks and months of the library’s future “but we’re transforming into something wonderful.” The support from the community has been overwhelming “Everyone has been helping.” As if to underscore this point the last patrons of the day offered to help carry boxes outside not the first change to affect the West Tisbury library and community support has been a defining characteristic of the library since its inception the West Tisbury Free Public Library was founded in 1891 by Nathaniel Southgate Shaler of Harvard University West Tisbury Congregational Church pastor William Rotch Judge Everett Allen Davis and wife Georgianna Davis But it wasn’t until 1893 when the founders purchased the Moses C Mitchell Family School for Boys located on Music street did the library finally have a place to call home The small mansard roof building served as the town’s library until 1992 when it relocated to its current location West Tisbury resident Allen Whiting has warm memories of the town library in both locations “The librarians over my lifetime have become heroes to the community,” he said Whiting was often “looking off into the sunset” rather than checking out books at the library but he noted how the town has always yearned for knowledge “I think it’s become a civic center at this point,” he said there’s no line outside of my gallery but there can be one outside the library.” Whiting has strong familial ties to the library was the librarian at the Music street location from 1970 to 1982 Whiting’s wife Lynn recalled how the library was an introduction to Island life when she first moved to West Tisbury from her home state of Utah that was our first social center,” she said “I used to go to the library and leave Bea in the bouncy seat in front of Nancy’s desk and feel comfortable and safe.” Whiting’s daughter would eventually become a library intern under Anne Fielder Each day after working at the library Bea would return home and try to organize the Whiting household under the Dewey Decimal system “Kids that grow up in that kind of environment have an exciting outlook on life,” Lyn Whiting said From 1895 to 1949 the second floor of the library housed the Martha’s Vineyard Historical Society Eventually this was converted to a children’s room “The children’s library was terrific because you could go out on a tiny deck and see the world around you on the second floor,” said Ann Fielder the children’s librarian during the 1980’s “I loved going there and it just seemed to me it was a refuge “When I was the children’s librarian the only thing I ever had to do was make sure the grown-ups weren’t up there chatting with each other while one child was reading to a table full of other smaller children,” she added “I may have come down heavy at times,” she admitted But not all memories were quite as positive author and longtime West Tisbury resident Cynthia Riggs who worked at the Music street library from the 1930s through the 1950s “[She] turned me off libraries for a long time,” Ms McNeill was a very stern librarian who felt books belonged in the library and shouldn’t be taken out and she felt that children had no business reading books that were inappropriate and made sure they wouldn’t get them,” Ms “She was so scary I stayed away from libraries until I was in college.” “But I always loved that little building — there were two great clam shells [sculptures] out front the kind you’re scared of stepping on because they’ll hold you under water.” Riggs said she is looking forward to the new library and is eager to see its evolving role on the Vineyard “It’s one of the most important things in the community,” she said especially in the way it’s adapting to the digital age because the library is going to be every bit as important with electronics as it has been in the past.” “I am 100 per cent in favor of libraries,” Ms it’s nothing but praise for the library staff and welcoming atmosphere at the library I go in there and there’s a real interest in you as a person,” West Tisbury poet laureate Justen Ahren said “They have a knack for knowing everyone there.” Ahren frequently uses the library to write his poetry; staff often suggest new titles for browsing and make space available for poetry readings “It’s amazing to have support under what you do and have it be considered a valuable thing,” he said “The library has done that for me and I’d venture to say it does that for a lot of people of all different walks of life — carpenter gardener or bird watcher — they support that and validate your interests.” the joint venture between Valio and energy company St1 has studied the possibility of launching a biogas plant project in the Ostrobothnia regions the company has decided to proceed with the planning of two new plants in the Pedersöre-Kruunupyy and Nivala-Sievi areas Suomen Lantakaasu Oy advances towards its production target of one terawatt-hour by 2030 Suomen Lantakaasu acquired a majority stake in Nurmon Bioenergia Oy The company is already developing a biogas plant project in Southern Ostrobothnia the site options for the main plant of the new biogas plant projects are limited to the Pedersöre and Kruunupyy municipalities in Ostrobothnia Further development in Northern Ostrobothnia will focus on the Nivala-Sievi area where the plant would be located along highway 28 Pedersöre-Kruunupyy and Nivala-Sievi are potential locations for the biogas plants The Pedersöre-Kruunupyy area has a large number of farms producing manure suitable as the raw material for biogas production The Pedersöre and Kruunupyy area is well suited to the construction of biogas plants because of the land available it is one of the largest agricultural clusters in Finland and the region has a strong green energy industry has a significant concentration of dairy farms with a high potential for biogas activities According to the company’s feedstock survey farmers in both regions are interested in becoming feedstock suppliers for Suomen Lantakaasu’s biogas plant “We are currently carrying out more detailed studies on the most suitable sites for construction of the biogas plants After the ongoing nature inventories and infrastructure studies have been completed we will be able to decide on the final location of the sites and start signing letters of intent in the specified areas,” says Leena Helminen with responsibility for the Suomen Lantakaasu business development Suomen Lantakaasu’s biogas business projects move forward Suomen Lantakaasu’s two previous biogas plant projects – at Nurmo in Southern Ostrobothnia and at Kiuruvesi in Upper Savo – are approaching the investment decision stage preparatory groundworks for the biogas plants are already underway and the plants are expected to become operational in 2026 Together with the Upper Savo and Nurmo projects Suomen Lantakaasu’s new projects to be advanced represent well over half of the one-terawatt-hour production target “With Suomen Lantakaasu’s production plant plans moving rapidly towards the one-terawatt-hour production target by 2030 To encourage transport companies to use biogas a comprehensive and steadfastly forthcoming refuelling infrastructure is critical so that transport companies and end customers can invest in low-emission heavy transport fleets Our growing network of distribution stations is helping our customers drive the energy transition and reduce emissions in both agriculture and transport,” says Matti Oksanen Suomen Lantakaasu has launched recruitments for the procurement of feedstock financial management and production management of the plants under construction Valio is a Finnish food company founded in 1905 Valio is owned by 3,400 Finnish dairy farmers through cooperatives We pay out our operating profit to the dairy farms The Valio brand is a promise of first-class quality and responsibility Valio’s sales in 2023 were EUR 2,3 million We are Finland’s biggest food exporter and a trailblazer for the whole food industry We export our consumer and industrial products to approximately 50 countries a company focusing on plant-based products Our Valio Aimo® wholesale business serves hotels and customers in the public sector and food industry all around Finland a joint venture we established with energy company St1 is boosting the circular economy by producing biogas from dairy farm manure. In all Our goal is to cut the carbon footprint of milk to zero by 2035 growing carbon sinks and creating circular economy solutions and we are working to increase biodiversity variety and joy to the dining table and professional kitchens we are building wellbeing and making sure that we will have locally produced food also in the future Innovativeness and drive for renewal have brought us at Valio together for over a hundred years − ever since Nobel laureate A.I Virtanen headed Valio’s product development Join the conversation You can save this article by registering for free here. Or sign-in if you have an account Speaking publicly for the first time since his brother’s death Arkangelo appeared at an emergency town hall meeting Friday night as community leaders and allies gathered to discuss policing and fears of police brutality toward racialized people Subscribe now to read the latest news in your city and across Canada Create an account or sign in to continue with your reading experience “In my head I just see my baby brother — because that’s how he’ll always be to me — and then him on the ground shaking and waiting for help who had to be consoled by members of his family as he spoke for roughly three minutes “I hope no other family ever has to go through this again I don’t know how we’re going to get over this.” His family says they have many questions that have so far gone unanswered The chain of events leading to the shooting began when the 28-year-old was involved in a rollover on Anthony Henday Drive and 153 Avenue around 9 p.m ASIRT said Arkangelo was seen by witnesses leaving the vehicle prompting officers to search for him in the northeast Edmonton neighbourhood of Fraser the Edmonton Police Service Air 1 helicopter team arrived in the area an officer on the ground called in to say he had located a man that matched the description of the driver By signing up you consent to receive the above newsletter from Postmedia Network Inc The next issue of Headline News will soon be in your inbox Interested in more newsletters? 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The agency said Arkangelo walked toward the officer’s cruiser with its lights flashing and the officer drove his vehicle toward the man the officer stopped his cruiser and stepped out with his gun drawn while Arkangelo stopped walking and raised his arms to his sides The two men “engaged verbally” with each other before the officer fired his weapon at Arkangelo Arkangelo received medical attention from emergency crews when they arrived but he died at the scene as a result of gunshot wounds Edmonton police described the exchange between Arkangelo and the officer as a “confrontation.” we’re just asking what happened between the police and him Why didn’t they take more time in that situation?” said Arkangelo’s cousin Nivala Uka “We don’t want to take it as a racial thing Arkangelo has been described as a fun-loving who had a passion for music and was a doting father to his seven-year-old son but had dreams of being a full-time musician Uka said her family always enjoyed listening to him perform Uka said Arkangelo was an amazing father who did everything for his son but now the boy will grow up without a father They were always doing things together,” said Uka We’re preparing ourselves for the day of the funeral when he sees his dad lying in the coffin protect him and be there for him and we’re going to pray that justice will come.” said he has many questions about police actions the night of June 29 Engel said video footage indicates Arkangelo was surrendering to police and wasn’t posing any kind of threat that would’ve given the officer reason to shoot “Both hands were raised and he was showing the officer what he had It was a 100 per cent surrender posture,” said Engel Criminologist and former EPS officer Dan Jones said ASIRT cases often take months or even years to close “You have this grieving family and they want answers and they’re not going to get answers for quite a while Those are things I struggle with,” said Jones transmission or republication strictly prohibited This website uses cookies to personalize your content (including ads), and allows us to analyze our traffic. 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By continuing to use our site, you agree to our Terms of Use and Privacy Policy You can manage saved articles in your account HELSINKI FINLAND JUser: :_load: Unable to load user with ID: 42 Why couldn’t the cows in Finland embrace the Nordic/Scandinavian morals and just go Woodstock I was soon to learn that it was indeed true - dairy cows in the countryside wore bras for udder support Although it wasn’t my first visit to Finland there was much I’d learnt in the past 3 months living in the suburbs of Nivala as an intern for a mining company described by Wikipedia as unilingually Finnish with a bustling population of close to 10,000 people although I’m sure the most I’ve ever seen at once is 100 It was there that I got my first taste of Finnish cuisine drank first of many sauna beers and spectated in competitive sports such as laptop throwing and high-heel relays where camps for exploration geologists are anything from actual camps (with tents to cabins (the latter two having limited Internet connectivity) I came to find my quarters in Nivala more than liveable it had a sauna - a feature I was soon to learn was considered a necessity in all Finnish houses Although I was no stranger to the idea of showering sweating your skin off and then repeating the process the idea of taking beer into the sauna was one I hadn’t really taken too seriously It was recommended to me by a friend at work and on a weekend when I was home alone with little to do I decided to do some Internet research and try it Less than 10 minutes into a second bottle of Karhu I remember feeling pretty pleased with life my preconceived notion about Finns not being overly sociable was proved false when my work friend invited me over to spend the weekend with him his wife and a couple of their friends- all of whom were extremely chatty I visited a javelin museum in the wee lakeside town of Pihtipudas learnt that the stereotype about Finns and potatoes was indeed true (that they eat a lot of them I mean) and got my first taste of Jalokola The latter is a cocktail made with Jaloviina (Jalo) Another tick off my bucket list was sampling the local cuisine I sampled homemade blueberry pie or mustikkapiirakka and reindeer meat with lingonberries The highlight of my summer in central Finland was Men and women alike competed in their respective categories for the title of “Best Laptop Thrower” Approaching a throwing platform (like you would expect to see for discus or shot put) most competitors had a glove on their throwing hand and selected their laptop by weight they would fling the duct-taped laptop across the field And depending on if the throw was considered a success or a failure The winning throw in the men’s category was over 30 m Women in teams of four sprinted in their heels and as something else that I’m still not certain of While their costumes were highly entertaining there was no standardisation of heel length or shape Should there have been a heel committee I would have motioned for the dismissal of wedges and a heel shape and length under the “pump” and “four inch” categories respectively Although Nivala wasn’t the most hopping of towns I thoroughly enjoyed my time there and shall miss it sorely I’d definitely suggest hitting up the pizza parlour and the statue of the milkmaid and the cow a visit during the laptop throwing season would be recommended Advertisement inquiries and other after-sales issues: info@helsinkitimes.fi Helsinki Times is the first and only English language newspaper providing news about Finland in English A weekly print edition of Helsinki Times was published from March 2007 up until Feb Helsinki Times is an online-only publication and other groups and individuals interested in Finland from all around the world © Helsinki Times All rights reserved.  Terms of Use | Privacy Policy That’s good news to the CEO of a Finnish company involved in one of them Heikki Nivala is the head of Boreal Bioref a planned billion-euro biorefinery project in Lapland’s Kemijärvi a nationally-recognised champion of the forestry industry said he doesn’t agree with researchers who’ve issued warnings about Chinese advancements and investments in the country’s forests the forests are growing quickly and need to be thinned,” he said “We are just about ready,” he said of the project “The only things we need now are environmental and building permits the plant will be built in 30 months,” Nivala explained Nivala spearheaded a grass-roots protest movement after paper firm Stora Enso announced it was shutting down a cellulose plant in Kemijärvi in 2007 The facility was closed the following year but the town’s – and Nivala’s – name continues to be remembered by many in the country The town suffered many job cuts when the factory closed but because of the prospect of Chinese investments Boreal Bioref has estimated that its planned facility will bring 1,000 new jobs to the little community The firm has already inked contracts of intent with the Chinese and the facility’s environmental permit application is expected to be processed this spring Not everyone is excited about increased activity in the forestry industry He’s a history and geography studies researcher at the University of Eastern Finland has studied the forest industry’s past present and future – and said he is worried about looming growth in the industry “If all of the projects proceed to full-scale efforts forestry usage levels will rise by 15 million cubic metres and pulp production by three million tonnes I’ve only put together the capacity and raw materials figures the companies have announced,” Donner-Amnell said Finland cleared some 77 million cubic metres of forests but adding the anticipated output of the five upcoming projects that sum would rise to some 95 million cubic metres “That’s 11 million [cubic metres] more than recommended by Luke (the Natural Resources Institute) as Finland’s highest sustainable felling level,” Donner-Amnell explained noting that each of the five projects’ environmental impact surveys have been carried out individually He said the combined environmental impact of the projects has not been considered “This is interesting because none of the five projects has been given a final green light yet the decisions for all of them are expected this or next year,” he said Donner-Amnell said the new government will have to decide on the projects because the impact they will have on the forestry industry will be far-reaching and problematic Despite his misgivings about the forestry industry’s plans he said he is not accusing the Chinese of anything “It is our officials – read government and ministers – that are responsible for the entire forestry industry and [they] have the ability to make sure it doesn’t go overboard,” he said Donner-Amnell said that if the projects go ahead as planned the bio-based economy will pose threats to the country’s ecology and economic interests it is funding from China that is making the Boreal Bioref project viable He said he personally dealt with the Chinese investors and has visited the country several times the Chinese have centuries of experience behind them as deal makers They need raw materials and the forestry industry here in safe Nordic countries are very interesting to them,” Nivala said The Chinese state-owned construction firm China CAMC Engineering is Boreal Bioref’s longest-running partner and is involved as an investor but other Chinese players are also engaged in the project The business effort has also received funding from investors in several European countries Due to the fact the planned biorefinery will produce more electricity than it uses energy companies are also involved in the project “The Chinese have done a good deal of preliminary work they know what their needs are and that it’ll be worth their while to come here,” Nivala said China is in the process of carrying out an investment strategy across the Nordic region which is part of Chinese state-owned Beijing Capital Investment is setting up a 400 million euro fund for investments in the Nordic tech sector Nivala said that China is looking for access to raw materials and that processing those goods adds value locally then we’ll send our products to Europe It’s all about the market economy here,” Nivala said The plant in Kivijärvi is one of five major forestry-related projects in the works which are at least partially financed by Chinese investors Finland: Climate change casts doubt on future of Finland’s forest industry Norway: WWF urges Norway to protect its Arctic forests to help fight climate change For more news from Finland visit Yle News. Your email address will not be published. Required fields are marked * Save my name, email, and website in this browser for the next time I comment. The ceremony took place in the North Ostrobothnia municipality of Nivala in the same church in which Senator Kallio gave the speech one hundred years prior Kallio was the first White Guard politician to propose that the warring sides work towards reconciliation after the brutal civil war fighting came to an end He would later serve as the fourth President of Finland President Sauli Niinistö addressed the people gathered in Nivala "The events which took place a hundred years ago are still of relevance for Finland today and it is not insignificant how we account for the past Civil war is the worst thing that can happen to a nation Let it be a lesson to us to remember and preserve our stability at a time of turmoil in various parts of the world," he said He said Kallio's reconciliation speech was remarkable for several reasons: one of them being its timing The bloody Finnish Civil War had ended just a few weeks earlier and tempers were still high "[Kallio] gave the speech at a very difficult time the Finnish Parliament was still suspended executions prevailed in the country and the army held a kangaroo court based on martial law," the Finnish President said Niinistö said it would be a misrepresentation of history to say that Finland would have smoothly moved to a more inclusive governmental policy after the Civil War No copies of Kallio's groundbreaking conciliatory speech were saved and the newspapers of the era also did not mention it "It is an irony of history that the speech was not saved in its entirety for posterity Yet its core message is still strong: the requirement to build a Finland in which “there are no Reds and Whites but only Finns who love their fatherland citizens of the Republic of Finland who all feel themselves to be members of society and who are at home here” remains as engaging now as it was back in 1918," Niinistö said Prime Minister Juha Sipilä reminded the crowd that reconciliation did not come swiftly after the Finnish Civil War A violent cycle of bitterness and revenge was unleashed once the official fighting of the war had ended A reign of terror targeting the Reds in particular swept through Finland in the spring and summer of 1918 "Thousands of people were killed in post-war altercations and prison camps before the reconciliation policies began to be implemented in earnest Kallio preferred a policy of mercy over revenge," Sipilä said Finland's premier said Kyösti Kallio's speech at the Nivala church laid the foundation for Finland's future The next real turning point took place after the next municipal elections when rulers accepted the Social Democratic Party's participation in local government "This integration effort reached its fulfillment years later after Kallio was elected president and named both the winners and losers of the conflict to work side by side in the government," Sipilä stated Both Sipilä and Niinistö stated their concerns about increasing antagonism in modern-day Finland a phenomenon they say is growing on social media and internet discussion forums to take the responsibility," Niinistö said "Nurturing democracy is an invaluable tool in reconciling different points of view This is a good rule of thumb: even where there is diversity and people of different backgrounds This is something we must be able to respect however differently we ourselves might think This is what Kyösti Kallio urged his fellow citizens to do in his famous Nivala speech as well as consistently in his other actions is ice cream becoming a luxury?With inflation raising the cost of raw ingredients Finns are paying the price as they line up for their favourite summer-time treat Open image viewerAt the kiosk near Seurasaari a single scoop was 50 cents cheaper at 4.50 euros Image: Jani Nivala / YleYle News11.5.2023 15:19The sun is shining and the week is warming up as the days go by including one near Seurasaari island in Helsinki are welcoming their first customers of the season but says she has noticed the price of ice cream creeping up "Ice cream for me is a sort of luxury," she said "If I had three young children and needed to buy ice cream for all of them I would probably just get some cones from the store." Open image viewerEeva Taimela said she might get cones from the store if she was taking kids out for a treat Image: Jani Nivala / YleVendors under pressurePauliina Jussila has been selling ice cream for over 30 years She and her family operate several ice cream kiosks at different locations "We calculated that the price of the ice cream we sell has increased by an average of about 28 percent in two years," she told Yle "Everything has become more expensive Jussila said she hopes she won't have to raise the price of a scoop beyond 5 euros "The general price level is now around five euros per ice cream scoop Some vendors price it at 4.90 and some at 5.20 We thought about setting the price at 5.50 euros this summer but we decided that five euros is better," she explained Getting your ice cream fix at the supermarket is no doubt easier on the wallet a store-bought litre of ice cream will yield many scoops while a multipack of cones or popsicles is another option for satisfying a sweet tooth Espoo resident Katja Helenius said she has contemplated the price difference between store-bought and kiosk ice cream "Paying five euros for a single scoop can feel a bit steep considering you can get a couple of litres of ice cream from the store for that price But it's not like you're going to carry your own ice cream when you're out and about," she explained Eating an ice cream at Seurasaari is Jarmo Hirsto who said that in the scheme of things ice cream price fluctuations were relatively small "Ice cream is a part of summer," he said admitting that the cone he was licking wasn't his first of the season Finns have a taste for ice cream, with residents consuming over 12 litres of the milky treat on average every year.