A dog kennel owner has been handed a 10-year ban after allowing dogs in her care to suffer Watch more of our videos on ShotsTV.comand on Freeview 262 or Freely 565 Natalie Bailey, of Copthorpe Road, in Great Barr, who ran a private boarding kennel in Birmingham admitted a series of animal welfare offences against three dogs Bailey was investigated by the RSPCA investigators after concerns were raised by a member of staff at a vet when a bulldog was brought to the building in a suffering state The concerns were raised about the mistreatment of the dogs at the private kennels in Cookset Lane A staff member at the vet said that the English bulldog was bought in by a woman who was 'very panicked' and 'had blood over her arms' The staff member said: "A woman arrived at the clinic very panicked and had blood over her arms "She said that she had picked her dog up from the boarding kennels and that she thought she was dying I went straight out to the car and there were two bulldogs in the back seat she felt cold and was severely underweight The owner explained that the dog had been at a kennel for several weeks while she moved house The staff member said: "The owner explained to me that the dogs had been at a boarding kennel for several weeks whilst the owner was moving house "She said had been sent some photos while they were boarding The owner then went on to say that she rang the kennels as she was worried that they may have run out of food "The woman at the kennels said that they were fine the owner felt this was strange and so took a bag of food around anyway she found the dog in this condition so collected her straight away and brought her straight down to us." Rochea suddenly collapsed and had to be put to sleep with consent from her owner A female Staffordshire bull terrier-type dog named Brindy was also found to be suffering when she was presented in an emaciated state with the dog showing a history of vomiting to a different vet practice She was being cared for by Bailey while her owner was in hospital who investigated for the animal welfare charity said: "Owners entrusted Bailey with their pets expecting a level of care which they sadly did not receive.” was also found to have been left in a crate for a long period causing it to suffer scalding due to standing in urine and faeces Beau had also been found not to have been given appropriate food and kept in an unclean environment The 31-year-old was banned from keeping animals for 10 years after pleading guilty to offences under the Animal Welfare Acts of 2006 and was ordered to carry out 25 Rehabilitation Activity Requirement days when she was sentenced at Birmingham Insense Supervision court on April 10 Please enable JS and disable any ad blocker Get the best experience and stay connected to your community with our Spectrum News app. Learn More spoke with Spectrum News 1 in April for his first in-state television interview since launching his U.S During this "In Focus Kentucky" exclusive segment Barr discussed more about his decision to run for this office and campaigning on an "America First" agenda set by President Donald Trump You can watch the full In Focus Kentucky segment in the player above started the healthcare sales certification program three years ago; the program now boasts roughly 200 students interested in taking on roles in healthcare Barr said she was asked by Miami University administration to start the program with Stryker a medical equipment manufacturing company that creates a wide range of operating room products.  Through this program, students have the opportunity to interact and network with leaders in the healthcare industry around the world. For example, recently, Greg Roche, the CEO of the Swedish company Distalmotion came in and talked to students about his experience at Miami,  and his career path Barr said this program is what she is most proud of from her time at Miami.  “[Barr] gave a toast at the end of our dinner when we were touring Stryker She teared up and got emotional talking about how much the program has meant to her,” said Katie Kruzich a senior in the healthcare sales certificate program and a marketing major “You could really tell that this program is her baby Kruzich said she first met Barr in a professional selling class for marketing her sophomore year — she continued to have two more classes with Barr: Marketing 490C Kruzich said Barr encouraged her to go to a resume workshop a Stryker employee who also teaches at Miami Tavella instantly became a mentor for Kruzich and she said she hopes to continue to work with him in the future after graduation.  “I talk to Jeff now more than I talk to Barr,” Kruzich said Kruzich said Barr has also influenced her in the classroom After telling Barr about a job her mom got at a medical testing company “[Barr] really cared about not just me but also my family,” Kruzich said “She was just really interested in her students’ lives.”  Along with starting the healthcare sales certificate program Barr also helped develop the winter term study abroad programs This is where senior Ben Hanchar met Barr for the first time a chemistry major with a minor in business said he started the healthcare sales certificate program because of Barr.  “I wasn't really sure what I wanted to do with my career,” Hanchar said Barr taught Hanchar how to network and connected him with mentors.  you always have to be networking,” Hanchar said “Your network is what's going to get you to jobs and careers.”  and Marketing 490C with Barr and started attending her office hours Barr taught Hanchar to network and connect with other people professionally.  “She's just been so helpful,” Hanchar said “She is always willing to go the extra mile and help you outside of class.” mulforsj@miamioh.edu — One of the top high school basketball rivalries in the state of Indiana took an unexpected turn Barr-Reeve coach Josh Thompson is set to become the next coach at Loogootee The move will become official at a special school board meeting on Wednesday Thompson had been at Barr-Reeve for seven seasons winning the IHSAA Class 1A state championship in 2021 More: Rusty Seymour steps down as North Posey basketball coach after five seasons Barr-Reeve and Loogootee are separated by eight miles predominately along U.S "I want to thank everyone at Barr-Reeve including the administration but most of all the past/current players and assistant coaches," Thompson said in a statement "It is the efforts and dedication of the players and coaches that have made what we accomplished so special We cannot thank Barr-Reeve enough for seven fantastic years my family and I are excited about the new opportunity at Loogootee I look forward to leading the basketball program and athletic department there into the future Christmas and the Board at Loogootee for their professionalism in working through this process over the last couple of weeks we are excited to move forward and what the future holds for us as Loogootee Lions." The move adds an extra spice to a rivalry that dates back a century one of two schools that consolidated into Barr-Reeve in 1965 Both schools are currently in Sectional 62 has a 261-192 record across 18 seasons at four schools Wood Memorial (he won back-to-back sectional championships in 2015 and 2016) and Vincennes Lincoln But his strongest run came with a move to Daviess County He never produced a losing season in seven years with the Vikings, winning four sectional and three regional titles. Barr-Reeve lost to Fort Wayne Blackhawk in the Class 1A state championship in 2019 and defeated Kouts in a return two years later The Vikings were among the favorites to win in 2020 before the state tournament was canceled due to the COVID-19 pandemic They also came within one victory of a trip to Gainbridge Fieldhouse in 2024 Barr-Reeve went 17-7 last season and lost to North Daviess in the sectional semifinal Thompson will be the seventh Loogootee coach since Hall of Famer Jack Butcher retired in 2002 Dave Smith stepped down following a 13-11 season Loogootee is scheduled to host Barr-Reeve in its annual regular-season meeting on January 16 Bison who are changing the world to make it a better place right now and for future generations The Kenya-born leader and first Black mayor of a Northern Ireland city will be honored with a Doctor of Laws degree during the 2025 Commencement “Some people have said it has been groundbreaking I believe I have been grounded in my pursuit to justice and [my] pursuit to creating equality and equal opportunities for everyone.” — Mayor Lilian Seenoi Barr the Kenya-born Mayor of Derry City and Strabane recognizes that her journey — full of great challenges as well as history making accomplishments — is an inspiration to many including those just beginning their own journeys It’s a responsibility she gladly accepts and one she takes with her daily as she firmly believes in the unique potential of others “That is what I want to do in my position now inspire more of our own community to believe that they can occupy those places of influence that they can also be decision makers,” said Seenoi Barr “That’s a weight I carry every day — to ensure that I succeed in any mission that I do; not just to prove to myself that I can do it but also to others who may be doubting themselves that you can also do it What she did was become the first Black person to be elected to public office in Northern Ireland elected to the Derry City and Strabane District Council’s Foyleside ward in 2023 The Mayor of Derry City and Strabane District Council is an honorary role with the recipient chosen by other members of the council to serve for a one-year term The mayor acts as the council chairperson and the city’s “first citizen.”    her path to leadership started long before her immigration to the country she now proudly calls home and public service began when she was a young girl in Kenya “I’m a Maasai woman and had an amazing upbringing “Everything I do has always been driven by purpose and the people.”  Seenoi Barr was given the opportunity to be educated by parents who valued education as “a way to change and transform your life and also your society.” Experiencing the traditions within the Maasai culture — including young children in addition to the possibility of forced marriage and female genital mutilation — she felt a responsibility to fight for her community and girls who looked like her “I felt that sense of responsibility to fight for the Maasai girl child and that is where my activism began,” she said.  That activism continued as Barr became a prominent force in the fight for gender rights among Maasai women eventually forced to flee with her son for their safety due to that extensive work a refugee,” finding common ground in the challenges she faced at home with those of her new community and other people from my community or the refugee community really ignited the same fire I had in Kenya to continue fighting for their rights and their voice to be heard.”   In addition to her current role, Seenoi Bar founded and is chief executive officer of the North West Migrants Forum according to her biography on DerryStrabane.com she advocates for the “rights and integration of migrants and minoritised minorities,” developing programming to address issues like racial discrimination When asked to name an accomplishment she is most proud of the mayor emphasized helping young people prepare themselves for leadership positions through a mentoring program she initiated as well as her ability to build relationships to benefit her community “One of the things that I am so proud of is being able to forge relationships with our city and district,” she said.  An Honored Recipient of an Honorary Degree from Howard  “Being awarded an honorary degree for the work that I have done all my life it’s just a great honor,” said Seenoi Barr who will be presented with an honorary Doctor of Laws degree during the Howard’s 2025 Commencement ceremony “I am humbled; I feel so blessed to have been recognized.” In terms of her profession the political leader finds the honor validating and proof that she’s on the right path “It just confirms that everything I’m doing is right; that there are people within the whole world who recognize social justice is a must That we absolutely have to continue fighting for the rights of our people and to come from an institution that social justice is embedded in every corner of its work — it’s a great honor.”  Barr will be among four other distinguished professionals receiving honorary degrees from the institution during the commencement ceremony May 10 including acclaimed artist and Commencement orator LeVar Burton; Emery Neal Brown Ph.D.; American poet Natasha Trethewey; and the Honorable Barbara Lee whom Seenoi Barr is looking forward to meeting again along with all of her “new Howard University family.”  “I’m really looking forward to meeting all the honorees,” she said “I was so pleased to see Barbara Lee being honored,” she continued “If I can do half of the things that Barbara Lee has done I will be one of the proudest Black women in the world when it comes to facing challenges and adversity as well as showing up as a leader others can look up to Seenoi Barr leans into what she calls an “unwavering spirit” of resilience which carries her through times of uncertainty “It is resilience that has been more than a trait for me it has been that way of life,” she said of her journey from refugee to mayor of Derry This is a message she hopes to impart on those currently forging their own path you have surrounded yourself with amazing people who have nurtured you and supported you — continue doing that but also hold on to hope.” She also stressed the importance believing that you belong in the spaces you occupy and to therefore enter them bravely and with the conviction to make change happen.  We need to believe in ourselves that we can occupy those spaces we deserve to have positions of influence to deliver for our communities.”  Our public relations team can connect you with faculty experts and answer questions about Howard University news and events A.G. BARR p.l.c. (LON:BAG - Get Free Report) shares crossed above its 200-day moving average during trading on Friday The stock has a 200-day moving average of GBX 622.17 ($8.27) and traded as high as GBX 699 ($9.29) BARR shares last traded at GBX 687 ($9.13) with a volume of 92,216 shares trading hands Shore Capital reiterated a "house stock" rating on shares of A.G Read Our Latest Report on A.G. BARR The firm has a 50 day moving average of GBX 638.80 and a 200-day moving average of GBX 622.49 a quick ratio of 1.99 and a debt-to-equity ratio of 2.62 The company has a market cap of £767.68 million A.G. BARR (LON:BAG - Get Free Report) last released its quarterly earnings data on Tuesday The company reported GBX 39.77 ($0.53) earnings per share (EPS) for the quarter BARR had a net margin of 8.81% and a return on equity of 12.27% equities research analysts predict that A.G will post 43.7109104 EPS for the current year The business also recently declared a dividend May 8th will be paid a GBX 44.80 ($0.60) dividend The ex-dividend date of this dividend is Thursday insider Euan Sutherland acquired 11,990 shares of the firm's stock in a transaction dated Monday The shares were acquired at an average cost of GBX 675 ($8.97) per share with a total value of £80,932.50 ($107,580.09) Insiders bought a total of 12,063 shares of company stock worth $8,138,446 over the last 90 days Corporate insiders own 23.13% of the company's stock MarketBeat keeps track of Wall Street's top-rated and best performing research analysts and the stocks they recommend to their clients on a daily basis. MarketBeat has identified the five stocks that top analysts are quietly whispering to their clients to buy now before the broader market catches on.. BARR currently has a Buy rating among analysts top-rated analysts believe these five stocks are better buys View The Five Stocks Here With the proliferation of data centers and electric vehicles the electric grid will only get more strained Download this report to learn how energy stocks can play a role in your portfolio as the global demand for energy continues to grow Sign up for MarketBeat All Access to gain access to MarketBeat's full suite of research tools The Magazine of the Science Fiction and Fantasy Field He was nominated for the Hugo Award for best fan artist five times and was twice a nominee for best professional artist While he did little in the way of formal training he was mentored by artists Mary Kimball Johnson and Harold “Jack” Vigos Barr began publishing illustrations in fanzines in 1959 (including work for Locus) and his first professional sale was the cover for the March 1961 issue of Fantastic His work appeared in many of the leading SF magazines and on covers for publishers including Ace and DAW Some of his work is showcased in Upon the Winds of Yesterday and Other Explorations (1976) and Paintings of George Barr (1976) He remained active as an artist into the early 2000s For more, see his entry in The Encyclopedia of Science Fiction Δdocument.getElementById( "ak_js_1" ).setAttribute( "value" For nearly 50 years Locus has been the benchmark of excellence the place our entire field looks to for information on what’s happening It’s been the best source of news and the best place for the people who buy books to find out about them It’s where we find out about the good Locus Magazine and Website cover science fiction and allows us to continue to share our content without a paywall Even a small contribution goes a long way toward keeping Locus going and keeping you up to date with new exciting books and in the know about the SFF world Metrics details evidence of age-related reactivation of a single gene on the female inactive X chromosome was observed in mice While stable silencing of the Barr body is crucial for balancing gene dosage between sexes it remains unclear whether silencing is maintained during aging Here we used allele-specific multi-omics approaches to capture a comprehensive catalog of genes escaping X chromosome inactivation throughout mouse development and aging We found substantially elevated escape rates during aging across organs occurring in multiple distinct cell types and concentrated at distal chromosome regions chromatin accessibility was increased across multiple megabases at chromosome ends As several age-specific escapees are linked to human diseases their elevated expression in females might contribute to sex-biased disease progression observed during aging there has been no systematic investigation of Xi stability throughout the mammalian lifespan we used allele-specific transcriptomics in a highly polymorphic mouse model with skewed XCI to capture the escape landscape across the major organs throughout development and aging We found that aging led to a substantial increase in escape rates Age-specific escape correlated with increased chromatin accessibility at regulatory elements of escape genes These results provide an important basis for future investigations on the contribution of Barr body instability to sex differences in age-related diseases Chromosome map depicting the localization of escape genes detected in investigated organs across the X chromosome Encircled genes show putative cluster formation A heat map visualizing median AR of detected escape genes across major cardiac cell types fibroblasts (FB) and endothelial cells (EC) The color code matches the AR gradient described in e Localization of escapees detected in the whole heart (red) and major cardiac cell types the putative Ddx3x escape cluster is shown Overlap of detected escape genes between the whole heart and cardiac cell types Distances (Mb) between whole heart escape genes (located at 0 labeled in red) and cardiac cell-type-specific escapees (labeled in the corresponding cardiac cell type color) The pie chart quantifies the proportion of escape genes within and outside 2.5 Mb Mouse–human conservation (gray) was extracted from the Mouse Genome Informatics (MGI) and Ensembl databases A bar graph illustrating the proportion of disease-associated (red) and non-disease-associated (light blue) escapees and X-linked nonescapees A Fisher’s exact test revealed a significant enrichment of disease-associated genes among escapees (P = 1.43 × 10−8 A schematic overview of the snRNA-seq workflow Aged and adult hearts were isolated from wild-type BL6 × CAST females (n = 2 Nuclei were isolated and FACS sorted before generating the snRNA-seq libraries using 10x Genomics quality control and normalization the whole dataset was analyzed for population shifts To split the dataset according to the active X chromosome (Xa) a chromosome-wide Allelome.PRO2 analysis was conducted (AR on chrX ≤0.2: CAST Xa; AR on chrX ≥0.8: BL6 Xa) All downstream allele-specific single-nucleus analysis was performed on cells with BL6 Xa only UMAP dimensionality reduction of 14 cardiac cell clusters in adult and aged samples The cell-type composition in adult and aged samples CAST Xa (brown) and biallelic X-linked AR (gray) A violin plot depicting the AR of X-linked genes of the adult and aged sample obtained by an Allelome.PRO2 analysis using all BL6 Xa cells as a pseudobulk input Boxes show the interquartile range (IQR) around the median The AR of age-specific escape genes identified in the bulk RNA-seq heart Only cell clusters containing more than 50 BL6 Xa cells in both time points were analyzed (cardiomyocytes (CM) FB and EC were merged for allele-specific analyses Left: UMAP visualization showing AR of an example gene Med14 with the corresponding delta AR (∆AR) calculated as: AR adult − AR aged Right: the expression pattern of Med14 across cell clusters during aging The color intensity corresponds to the average expression while the dot size represents the percentage of cells within each cluster expressing Med14 Same as in g for age-specific escape gene Sh3kbp1 Dynamics of escape ATAC peaks across the adult and aged time point X-chromosome map showing the localization of peaks that are present in all replicates and have ∆AR ≥0.1 (∆AR Peaks with ∆AR ≥0.1 within 200 kb of an escapee are shown in blue while those beyond this distance are shown in gray The distance between escape genes and ATAC peaks (∆AR ≥0.1 within 200 kb of an escapee) with peak overlap indicated for promoters (red) Genome browser tracks showing the age-specific kidney escape genes Cldn2 (left) and Reps2 (right) Xi-specific reads (brown) and non-allele-specific reads (gray) for ATAC- and RNA-seq Green (escape) and red (nonescape) bars indicate the escape status for genes and ATAC-seq peaks along with their AR (AR ≤0.9 for escape cutoff) ENCODE candidate cis-regulatory elements (cCRES) are shown as: promoter SNPs used for assigning sequencing reads to Xa or Xi are shown in dark blue allele-specific characterization shows that the distal chromosome regions of the Xi become more accessible at sites of regulatory elements that might drive escape gene expression Whether telomere shortening plays a role in age-specific reactivation and how aging affects different layers of the epigenome One limitation of this study is that only 81% of X-linked genes can be assessed due to the presence of SNPs within the gene body the majority was not expressed in our dataset we were able to assess the escape status of a substantial fraction of expressed X-linked genes representing a robust dataset for further analysis Future studies will need to address the role of age-related escape genes in sex-specific disease progression during aging and whether they could be implicated in lifespan differences between sexes our systematic assessment of Barr body stability identified age-specific reactivation at distal chromosome regions The subsequent increase in expression of conserved and disease-relevant genes in females might provide additional insights into sex-specific disease mechanisms complementing the role of hormones in explaining sex biases in age-related disease All animal experiments were performed in accordance with relevant guidelines and regulations the German Animal Welfare Act and ARRIVE guidelines Approval was granted by the authorities (District Administration Department of the City of Munich F1 hybrids were euthanized via cervical dislocation and organs (brain spleen and muscle) were collected and flash-frozen in liquid nitrogen Organs were stored at −80 °C for further use Three replicates per time point and sex (BL6∆Xist × CAST females and BL6 × CAST males) were analyzed BL6 Xist−/+ females and wild-type CAST males were mated and females were checked for a vaginal plug the next morning lung and kidney) were collected and flash-frozen in liquid nitrogen Organs were stored at −80 °C for further processing Three replicates of BL6∆Xist × CAST females were analyzed 9-week- 1.5-year-old mice were homogenized in appropriate amounts of TRIzol reagent (1 ml TRIzol reagent per 50–100 mg tissue) using the GentleMACS Dissociator RNA_02_01 program One milliliter of homogenized organs was used for RNA isolation Frozen organs of E14.5 embryos were thawed in TRIzol reagent and homogenized using a pellet pestle RNA isolation was performed for all time points according to the manufacturer’s instructions (Invitrogen RNA concentration was measured by the NanoDrop Microvolume Spectrophotometer 100 ng of RNA was used to generate poly-A captured RNA-seq libraries using lllumina’s Stranded mRNA Prep Ligation kit according to manufacturer’s instructions Concentration and fragment distribution of completed RNA-seq libraries was measured on Agilent’s TapeStation System Libraries were sequenced in the 50-bp paired-end mode on a NovaSeq6000 at Helmholtz Munich the cannula with the heart was attached to the perfusion pump system and the heart was perfused with perfusion buffer for 1 min (4 ml min−1) the heart was rinsed with a digestion buffer for 10 min (recirculating 4 ml min−1) to enzymatically dissociate the ventricular cells atria were discarded and the ventricles were roughly homogenized in 2.5 ml digestion buffer by cutting with scissors A 1-ml syringe was used to shear the tissue pieces up and down 30 times the syringe sheared the liquid again 30 times the suspension was filtered through a 100-µm strainer and put on ice immediately frozen on dry ice and stored at −80 °C for further use sorted major cardiac cell types were resuspended in 1 ml TRIzol and RNA was isolated according to manufacturer’s instructions (Invitrogen 10 μg of RNase-free glycogen was used as a carrier and isopropanol precipitation was performed at −20 °C overnight RNA quality was measured on Agilent’s TapeStation system 0.5–3 ng of RNA were used to generate RNA-seq libraries using Takara’s SMART-Seq v4 PLUS kit according to the manufacturer’s instructions The concentration and fragment distribution of completed RNA-seq libraries was measured on Agilent’s TapeStation system this allowed us to include cardiomyocytes in our snRNA-seq analysis that as viable cells do not fit into the 10x Genomics microfluidic channels it reduced the mitochondrial read fraction in ATAC-seq experiments the nuclei extraction buffer (Miltenyi) was used according to manufacturer’s instructions the integrity of isolated nuclei was assessed on the Countess II FL Automated Cell Counter equipped with the DAPI EVOS light cube 50,000 nuclei of 2 replicates (BL6∆Xist × CAST females) per time point were taken as input for Active Motif’s ATAC-Seq kit and libraries were generated according to manufacturer’s instructions ATAC libraries were sequenced on a NovaSeq6000 at Helmholtz Munich nuclei of 1 replicate per time point (BL6 × CAST females) were incubated with DRAQ7 for 5 min before sorting Sorting the DRAQ7-positive population ensured a debris-free nuclei suspension which was again assessed on the Countess II FL Automated Cell Counter equipped with the DAPI EVOS light cube Single-nucleus libraries were generated using the Chromium Next GEM Single Cell 3′ Reagent Kit v3.1 (10x Genomics) with a target of 10,000 nuclei per sample The single-nucleus libraries were prepared according to the manufacturer’s instructions Sequencing was performed on a NovaSeq6000 at Helmholtz Munich TPM were computed by a custom R script to compare male and female expression levels which were then counted within 10-Mb windows along the entire genome The median number of peaks per window was calculated across replicates and the enrichment of aged peaks was calculated by dividing the number of aged peaks by the number of total peaks (aged + adult peaks) To test for significance of peak enrichment during aging assuming an equal number of peaks between the time points covering 20,635,313 SNPs between CAST and BL6 strains A minread (minimum number of reads covering a SNP) cutoff value of ≥1 and a total read cutoff value (total read count covering a gene) of ≥20 were applied For the allele-specific RNA-seq analysis of major cardiac cell types the same annotation was used in combination with a SNP dataset containing only SNPs located in exonic regions a minread cutoff value of ≥1 and a total read cutoff value of ≥10 were applied Our escape detection workflow identified X-linked genes with an AR ≤0.9 in females as escape genes X-linked genes with an AR ≤0.9 in males were excluded from the analysis including the following genes from the pseudoautosomal region: Mid1 G530011O06Rik and Gm39551; and four additional false-positive genes outside the pseudoautosomal region: Gm14719 In combination with the complete SNP dataset also used for RNA-seq data from organs Allelome.PRO2 was conducted with a minread cutoff value of ≥1 and a total read cutoff value of ≥20 the Allelome.PRO2 output was filtered for only those peaks that were called by MACS2 in their respective sample while the percentage of mitochondrial reads was simultaneously regressed out The Seurat objects were then integrated by Seurat’s IntegrateData() function and principal components were computed by RunPCA() using the top 40 dimensions for UMAP dimensional reduction and clustering individual cell clusters were manually annotated A custom-made chromosome-wide annotation together with a SNP dataset which excluded SNPs covering the Xist locus Only cells containing ≥10 X-chromosomal reads were further analyzed cells were then categorized on the basis of their AR (AR ≤0.2 CAST Xa To confirm age-specific escape genes in snRNA-seq cell clusters an allele-specific pseudobulk analysis was performed separating the CellRanger output bam file into pseudobulk bam files on the basis of their cluster and Xa status To compare the snRNA-seq results with our bulk RNA-seq obtained from BL6∆Xist × CAST females and to minimize strain-specific escape only cells with a BL6 Xa were analyzed as a pseudobulk The subsequent Allelome.PRO2 analysis used the same annotation as well as the SNP file as described for the whole-organ bulk RNA-seq data Only cell clusters containing more than 50 cells in both time points were analyzed For in vivo sequencing data like in this study as the experimenter has no influence on the results of these tests Normality of the data was tested using the Shapiro–Wilk test Statistical significance was assessed as indicated in the figure legends Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article All datasets have been submitted to the Gene Expression Omnibus (GEO) database and can be accessed under the accession code GSE274695. All other data are available from the corresponding author upon request. In addition, we provide access to the escape landscape resource via the Integrative Genomics Viewer at https://github.com/AndergassenLab/AgingX The relevant code to reproduce the analyses and figures of this study is available via GitHub at https://github.com/AndergassenLab/AgingX Gene action in the X-chromosome of the mouse (Mus musculus L.) Conservation of position and exclusive expression of mouse Xist from the inactive X chromosome A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome Gene regulation in time and space during X-chromosome inactivation Gene silencing in X-chromosome inactivation: advances in understanding facultative heterochromatin formation X-inactivation profile reveals extensive variability in X-linked gene expression in females Analysis of expressed SNPs identifies variable extents of expression from the human inactive X chromosome Landscape of X chromosome inactivation across human tissues Global survey of escape from X inactivation by RNA-sequencing in mouse Tumor-suppressor genes that escape from X-inactivation contribute to cancer sex bias Illuminating the mechanisms underlying sex differences in cardiovascular disease Peeters, S. B., Posynick, B. J. & Brown, C. J. Out of the silence: insights into how genes escape X-chromosome inactivation. Epigenomes https://doi.org/10.3390/epigenomes7040029 (2023) Escape from X inactivation varies in mouse tissues Andergassen, D. et al. Mapping the mouse allelome reveals tissue-specific regulation of allelic expression. eLife https://doi.org/10.7554/eLife.25125 (2017) Age related reactivation of an X-linked gene A proximal conserved repeat in the Xist gene is essential as a genomic element for X-inactivation in mouse a pipeline to define allele-specific genomic features from high-throughput sequencing data Hasenbein, T. P., Hoelzl, S., Engelhardt, S. & Andergassen, D. Allele-specific genomics decodes gene targets and mechanisms of the non-coding genome. Preprint at bioRxiv https://doi.org/10.1101/2025.03.03.641135 (2025) The International Mouse Phenotyping Consortium: comprehensive knockout phenotyping underpinning the study of human disease Alliance of Genome Resources Consortium. Updates to the Alliance of Genome Resources central infrastructure. Genetics https://doi.org/10.1093/genetics/iyae049 (2024) Regulation of X-chromosome inactivation by the X-inactivation centre Single-cell transcriptomics reveals cell-type-specific diversification in human heart failure and Dxz4 in vivo uncovers diverse phenotypes and combinatorial effects on autosomes Genetic architecture of skewed X inactivation in the laboratory mouse Andergassen, D. et al. In vivo Firre and Dxz4 deletion elucidates roles for autosomal gene regulation. eLife https://doi.org/10.7554/eLife.47214 (2019) Diverse epigenetic mechanisms maintain parental imprints within the embryonic and extraembryonic lineages Xist-dependent imprinted X inactivation and the early developmental consequences of its failure LINE-1 activity in facultative heterochromatin formation during X chromosome inactivation Age-related reactivation of an X-linked gene close to the inactivation centre in the mouse The inactive X chromosome accumulates widespread epigenetic variability with age Exploratory analysis of age and sex dependent DNA methylation patterns on the X-chromosome in whole blood samples Ageing-associated changes in DNA methylation in X and Y chromosomes An epigenome-wide association study of sex-specific chronological ageing Telomere position effect regulates DUX4 in human facioscapulohumeral muscular dystrophy Long-range telomere regulation of gene expression: telomere looping and telomere position effect over long distances (TPE-OLD) Telomere shortening relaxes X chromosome inactivation and forces global transcriptome alterations Dynamics of gene silencing during X inactivation using allele-specific RNA-seq Clustered transcripts that escape X inactivation at mouse XqD Deletion of an X-inactivation boundary disrupts adjacent gene silencing A whole-organism landscape of X-inactivation in humans Altered X-chromosome inactivation predisposes to autoimmunity TLR8 escapes X chromosome inactivation in human monocytes and CD4+ T cells Souyris, M. et al. TLR7 escapes X chromosome inactivation in immune cells. Sci. Immunol. https://doi.org/10.1126/sciimmunol.aap8855 (2018) Comparison of late-onset and non-late-onset systemic lupus erythematosus individuals in a real-world electronic health record cohort Aging activates escape of the silent X chromosome in the female mouse hippocampus The angiotensin-converting enzyme 2/angiogenesis-(1-7)/Mas axis confers cardiopulmonary protection against lung fibrosis and pulmonary hypertension Incidence and prevalence of idiopathic pulmonary fibrosis Mortality from pulmonary fibrosis increased in the United States from 1992 to 2003 Andrews, S. FastQC: a quality control tool for high throughput sequence data. Babraham Bioinformatics http://www.bioinformatics.babraham.ac.uk/projects/fastqc (2010) Reference sequence (RefSeq) database at NCBI: current status HTSeq—a Python framework to work with high-throughput sequencing data SNPsplit: allele-specific splitting of alignments between genomes with known SNP genotypes The ENCODE Blacklist: identification of problematic regions of the genome The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data Mouse genomic variation and its effect on phenotypes and gene regulation BEDTools: a flexible suite of utilities for comparing genomic features Massively parallel digital transcriptional profiling of single cells Unsupervised removal of systematic background noise from droplet-based single-cell experiments using CellBender multimodal and scalable single-cell analysis DoubletFinder: doublet detection in single-cell RNA sequencing data using artificial nearest neighbors Comparison and evaluation of statistical error models for scRNA-seq Stuart, T. & Kretzschmar, W. W. Sinto: single-cell analysis tools. GitHub https://github.com/timoast/sinto (2020) Download references We thank the Genomics Core Facility at Helmholtz Munich for performing the sequencing Bomhard for performing retrograde perfusion procedures Schindela for technical support during the revision and Life Science Editors for comments on the manuscript This work was funded by the DZHK (Deutsches Zentrum für Herz-Kreislauf Forschung) Junior Research Group grant this project was funded by the European Research Council (ERC-StG this work was supported by the Deutsche Forschungsgemeinschaft (Project-ID: 403584255—TRR 267) and the BMBF in the framework of the Cluster4Future program CNATM (Project ID: 03ZU1201BA) Open access funding provided by Technische Universität München Stefan Engelhardt & Daniel Andergassen DZHK (German Centre for Cardiovascular Research) and D.A.; investigation: S.H.; resources: S.E and D.A.; writing—review and editing: S.H. and D.A.; visualization: S.H.; supervision: D.A.; project administration: D.A.; funding acquisition: D.A The authors declare no competing interests Nature Aging thanks Andrew Lane and the other reviewer(s) for their contribution to the peer review of this work Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations (a) Violin plots showing the median allelic ratio of X-linked genes in male organs Detected escape genes in male tissue were categorized as false positive escape genes and deducted from the female escape analysis (b) Chromosome map depicting the localization of false positive escape genes as well as genes within the pseudoautosomal region (PAR) detected in male (c) Overview of major cardiac cell type isolation workflow A retrograde perfusion created a single cell suspension of the heart Cardiomyocytes (CM) were separated by centrifugation at 100 x g for 1 min creating a CM pellet while non-CM cells remain in the supernatant Macrophages (MP) were separated from fibroblasts (FB) and endothelial cells (EC) by CD45 magnetic selection via AUTOMACS Cell marker proteins and cell viability were stained for subsequent cell sorting by FACS (n = 3 per cell type) A heat map depicting row Z-scores of marker genes for the corresponding cell types (d) Representative example for the sorting strategy of MP CD45 + CD11b + CD64 + F4/80+ singlets were sorted (e) Representative example for the sorting strategy of EC and FB (f) Violin plots showing the median allelic ratio of X-linked genes in female major cardiac cell types (a) Unsupervised clustering of a Spearman correlation expression matrix (log10(TPM+1)) across 15 embryonic (b) The number of escape genes across all investigated organs is shown for embryonic (gray) (c) Xist expression levels (measured in TPM) are plotted against the number of escape genes for adult (light blue) and aged (dark blue) organs Each data point represents an individual organ with Spearman correlation coefficients calculated separately for adult (ρ = −0.1853) (d) Bar plot showing all aged organs ranked by their number of expressed X-linked genes The percentage of escape is indicated in green Pie chart showing the mean percentage of escape across all investigated organs at the aged time point (e) Correlation of TPM fold change (female / male) and allelic ratio (AR) of escape genes in the respective organs at the aged timepoint Normal distribution of the data was assessed using the Shapiro-Wilk test and the correlation coefficient ρ was subsequently calculated using the Spearman method Representative example for the gating strategy of isolated cardiac nuclei Nuclei were identified based on size and granularity and DRAQ7+ nuclei were sorted for downstream snRNA-seq (a) Dot plot showing marker gene expression across cell clusters in the heart at the adult and aged time point The color gradient represents the average expression across the population while the size of the circle represents the percentage of cells in which this gene is expressed (b) UMAP dimensionality reduction of 14 cardiac cell clusters in adult and aged samples containing only cells with BL6 active X chromosome (Xa) (c) Cell-type composition in adult and aged samples containing only cells with BL6 Xa (d) Escape gene expression across cell clusters in the heart at adult and aged time points containing only cells with BL6 active X chromosome (Xa) (a) Schematic overview: The allelic ratio of pseudobulk data could reflect a homogeneous escape pattern across all cells within a population or could represent the average of heterogeneous escape patterns While pseudobulk analysis combines reads from all cells allele-specific analysis at the single-cell level faces challenges such as low read counts per cell a deep sequencing approach may be required to obtain reliable results in single-cell allele-specific analyses (b) TPM expression levels of the variable escape gene Smpx in males (n = 3 (c) UMAP visualization of allele-specific analysis of Smpx at single-nucleus resolution Gray cells represent non-informative cells where the SNP-overlapping total read cutoff ≥ 10 was not met The color gradient illustrates the allelic ratios of Smpx for each single nucleus (d) Proportion of cardiomyocytes that are informative (blue) and non-informative (gray) in allele-specific analysis at the single-nucleus level (a) UMAP visualization showing the z-score of average expression of six commonly used senescence marker genes (Cdkn1a (b) UMAP visualization displaying expression levels of the lncRNA Xist (c) Co-localization plot showing cells positive for both Xist and senescence markers (yellow) Senescent-positive cells were defined as having a z-score of average expression ≥ 2 while Xist-positive cells required an expression level ≥ 2 (d) Box plot showing Xist expression levels of senescent and non-senescent cells and statistical significance between non-senescent and senescent cells was assessed by Mann–Whitney U test young and adult escape genes (located at 0) and age-specific escapees Dots indicate age-specific escape genes within (red) and beyond (gray) 2.5 Mb of identified escape regions (b) Proportion of age-specific escape genes within (red) and outside 2.5 Mb (gray) of identified escape regions (c) Chromosome-wide visualization of age-specific escape gene counts (top panel) and LINE element counts (bottom panel) calculated using sliding windows of 2.5 Mb corresponding to a 5% significance level (p = 0.05 was applied to determine statistical significance (indicated by asterisks) Red boxes highlight specific regions where most age-specific escape was identified (a) The bar plot illustrates the fraction of ATAC peaks for autosomes (Chr1–Chr19) as well as the active (Xa) and inactive (Xi) X chromosomes in adult (light blue) and aged (dark blue) kidneys (b) Chromosome map showing the pseudoautosomal region (PAR red) and the localizations of escape genes identified in adult and aged kidneys (top) Escape genes labeled in black are age-specific while escape genes labeled in gray were detected in adult samples Distribution of biallelic ATAC peaks along the X chromosome is shown for the aged kidney (middle) The bar plot illustrates the count of aged biallelic ATAC peaks within 1.5 Mb sliding windows corresponding to the size of the PAR (below) (c) Boxplot depicting the distribution of informative aged biallelic ATAC peak counts within 1.5 Mb windows (d) Allelic ratios of genes adjacent to the PAR in aged kidney samples spanning from the most distal escape gene (Vegfd) to the most proximal PAR gene (Mid1) (e) A heat map showing median allelic ratios of ATAC peaks in adult and aged kidneys Delta median allelic ratios (∆AR = AR_adult - AR_aged) were calculated with the top ∆AR peaks (∆AR ≥ 0.1) indicated by arrows Genome browser tracks depict allele-specific ATAC-seq data showing age-related accessibility patterns near the identified age-specific escape genes Otc (left) and Tsc22d3 (right) and non-allele-specific data (gray) are shown Green and red bars below the genome browser tracks represent escape and nonescape ATAC-seq peaks respectively (AR ≤ 0.9 as the escape cutoff) ENCODE candidate Cis-regulatory elements (cCREs) are shown as follows: promoter (red) Supplementary Table 1 Multi-omics investigation to map and characterize gene escape during mouse aging Sample information from of all sequenced samples Expression levels (TPM) of X-linked genes across adult organs ARs of X-linked genes across cardiac cell types Expression levels (TPM) of X-linked genes across cardiac cell types ARs of X-linked genes across embryonic organs Expression levels (TPM) of X-linked genes across embryonic organs Expression levels (TPM) of X-linked genes across young organs Expression levels (TPM) of X-linked genes across aged organs Disease association of identified escape genes retrieved from IMPC Disease implications of identified escape genes retrieved from the Alliance of Genome Resources Consortium ARs of X-linked genes across snRNA-seq pseudobulks ATAC peak count analysis of adult and aged kidney and liver ARs of X-linked ATAC peaks in adult and aged kidney Download citation DOI: https://doi.org/10.1038/s43587-025-00856-8 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing newsletter — what matters in science It is with great sadness that we announce the passing of  William “Billy” Scircle South Carolina and graduated from Lexington High School Class of 1962 were the proud owners for many years of Independent Water Works in Lexington He is survived by his dearly loved wife of sixty years Morgan Scircle; and his newest little gift “Billy” was predeceased by his parents Services will be held at 2:00 pm on Monday Visitation will be held one hour prior to the funeral at the church a Norstella company Pharma Intelligence UK Limited is a company registered in England and Wales with company number 13787459 whose registered A slice of the American South visited Portland this weekend with several Kentucky Derby viewings around town After horses nothing’s more closely associated with the iconic race than fashion And the attire has evolved in the century-and-a-half since the derby began in 1875 If you thought what people wore to the derby wasn’t a big deal, think again. The race’s official website has a whole page dedicated on tips and tricks for Derby Day attire. It even broke down the different kinds of hats: Cartwheel, Ladylike and Silver Screen Hollywood We couldn’t make it to Churchill Downs in Kentucky so we went to The Redd on Salmon event space in Portland instead Besides mint julep cocktails and horse betting Among the lavishly dressed crowd was a group of friends who claimed a prize for being the best-dressed team. What might have put them ahead? Perhaps the handcrafted hats made by team member Joanna Lukaszenko At least two of her hats have made it to the actual Kentucky Derby in Louisville Oregonian Media Group The material on this site may not be reproduced except with the prior written permission of the Oregonian Media Group Advertising Terms & Conditions: Use of and/or registration on any portion of this site constitutes acceptance of our User Agreement (updated 1/1/2021) and Privacy Policy and Cookie Statement (updated 5/1/2021) Advertising Terms & Conditions: Use of and/or registration on any portion of this site constitutes acceptance of our User Agreement (updated 1/1/2021) and Privacy Policy and Cookie Statement (updated 5/1/2021) Pardon our dust while we build a better museum! We are open with a series of special exhibitions. During construction, select permanent collection galleries in the Main Building will reopen on a rolling basis 2,000 Miles and Back:Traversing Changing Landscapes Portland-based artist and curator Zemie Barr will share recent photographic work made from 2021 onward in the midst of the COVID-19 pandemic and during a particularly devastating fire season for the Pacific Northwest These events led the artist to create multiple photo based series to process the grief associated with witnessing the effects of climate change in real time as well as analog and digital photographs taken while traveling across the country and beyond the artist balances these feelings of loss with an aesthetic focus on the sublime beauty and magical potential still present in the natural world Zemie Barr is an artist and curator based in Portland where she previously served as Exhibitions Director at Blue Sky Gallery and co-founded Wolff Gallery. Zemie uses digital and analog photography to explore themes of memory her work has been exhibited at Perspective Gallery in Evanston IL; Lightbox Photographic Gallery in Astoria OR; and in online exhibitions hosted by the Center for Fine Art Photography In 2022 she was selected as one of Photolucida’s Top 200 Critical Mass Finalists The Lunchtime Talk series is a presentation of the Portland Art Museum’sPhotography Council The Portland Art Museum recognizes and honors the Indigenous peoples of this region on whose ancestral lands the museum now stands Multnomah and Watlala Chinook Peoples and the Tualatin Kalapuya who today are part of the Confederated Tribes of Grand Ronde and many other Native communities who made their homes along the Columbia River We also want to recognize that Portland today is a community of many diverse Native peoples who continue to live and work here We respectfully acknowledge and honor all Indigenous communities—past future—and are grateful for their ongoing and vibrant presence The Portland Art Museum and Center for an Untold Tomorrow are pleased to offer accommodations to ensure that our programs are accessible and inclusive We’ll do our best to accommodate your needs when you arrive — please give us 2-3 weeks advance notice for specific requests Email requests to access@pam.org An official website of the United States government VA|News Veteran of the Day Happy National Park Week This week’s Honoring Veterans spotlight honors the service of Army Veteran and NPS employee Charles Barr Did you know that Veterans can receive a free lifetime pass to national parks The pass provides free entrance to Veterans and those traveling with them Just ask for it at the next national park you visit that has an entrance fee Charles Barr felt a call to serve his country The events of September 11 awakened a strong desire in him to join the military he enlisted in the Army Infantry in June 2005 and later transitioned to the 2nd Stryker Cavalry Regiment while on patrol during his second deployment to Iraq Barr returned to Iraq to complete his tour and continued to serve until a medical retirement in November 2012 Barr has served as the cemetery’s administrator Out of the 14 national cemeteries managed by the National Park Service Andersonville is the only one still open for internments Its hallowed grounds host approximately 200 burial ceremonies each year “Working at Andersonville National Cemetery has been one of my greatest experiences especially being able to help Veterans and their families during difficult times,” he said “There is nothing more rewarding than taking care of the final resting place of nearly 20,000 service members and their families.” The National Park Service is currently overseeing a $1 million rehabilitation and improvement project funded by the Great American Outdoors Act The project includes the thorough cleaning and realignment of every headstone conservation of all bronze figures and plaques and planting of hundreds of shrubs and trees helping to ensure that the historic landscape of Andersonville National Cemetery remains a dignified and honorable tribute to our Veterans #VeteranOfTheDay The comments section is for opinions and feedback on this particular article; this is not a customer support channel. If you are looking for assistance, please visit Ask VA or call 1-800-698-2411 never put personally identifiable information (SSAN etc.) or protected health information into the form — it will be deleted for your protection thank you for your continued service to our nation Charlie must be looking down on you with great pride I was blessed to work with Charles for a few years and his dedication to Andersonville National Cemetery is second to none and website in this browser for the next time I comment Honoring Veterans: Army Veteran Cruz Roque-VicensThis week’s Honoring Veterans Spotlight honors the service of Army Veteran Cruz Roque-Vicens Roque-Vicens went on to have a successful career as a sports journalist This week’s Honoring Veterans Spotlight honors the service of Army Veteran Albert Tristan This week’s Honoring Veterans Spotlight honors the service of Marine Corps Veteran Rodney Sickmann Get more resources at VeteransCrisisLine.net An official website of the U.S. Department of Veterans Affairs Looking for U.S. government information and services?  Visit USA.gov David C Barr","givenName":"David","familyName":"Barr","additionalName":"C.","birthDate":"1952-10-05","deathDate":"2025-03-11","deathPlace":{"@type":"Place","address":{"@type":"PostalAddress","addressLocality":"Decatur","addressRegion":"IL","postalCode":"62521","addressCountry":"US"}}}{"@context":"https://schema.org","@type":"NewsArticle","image":{"@type":"ImageObject","url":"https://cdn.f1connect.net/photo/tributes/t/8/r/207x207/8040327/David-Barr-1741875866.jpg"},"headline":"David C Visitation & Funeral Information","description":"Honoring the memory of David C visitation and funeral information.","articleSection":"Obituaries","articleBody":"David C on Tuesday (03-11-2025) with his family by his side.\n\nDave was born on October 5 California at the Army base Camp Cook to Robert “Bob” and Jewel (Langley) Barr IL.\n\nHe started his career at OK’s Transmission and then worked and retired from Caterpillar after many years He was a great mechanic and tried to help as many people as he could.\n\nDave enjoyed riding his Harley Davidson and was always on the go He was a 30-year friend of Bill W and was very active in that community He loved being with family and friends teasing and joking around.\n\nHe is survived by his wife Gale of 55 years; children: David C Lauren and Andrew; 6 great-grandchildren; his mother Jewel of Decatur IL; brother: John Barr and wife Julie of California; sister: Kaye Barr of Oregan; and brother: Jim Barr and wife Linda of The Villages FL.\n\nDave was preceded in death by his father Robert “Bob”.\n\nA Funeral Service will be held at St Monday (03-17-2025) with visitation from 10:00 A.M at the church.\n\nThe burial will be at Mt Gilead Cemetery following the funeral service.\n\nMemorials may be made to: St IL.\n\nCondolences may be shared with the family at www.gracelandfairlawn.com.","keywords":"David C Biography","dateCreated":"2025-03-12T17:52:35.65Z","datePublished":"2025-03-12T17:52:35.65Z","dateModified":"2025-03-13T19:24:25.16Z","genre":"Obituary Biography","author":[{"@type":"Organization","name":"Graceland/Fairlawn Funeral Home Cemeteries & Cremation Center","url":"https://www.gracelandfairlawn.com/"}],"mainEntityOfPage":"https://www.gracelandfairlawn.com/obituaries/david-barr-18/obituary","publisher":{"@type":"Organization","name":"Graceland/Fairlawn Funeral Home Cemeteries & Cremation Center","logo":{"@type":"ImageObject","url":"https://cdn.f1connect.net/cdn/14449D--Tg/layout/images/logo.63782426891.png"}},"creator":"Graceland/Fairlawn Funeral Home Cemeteries & Cremation Center","isAccessibleForFree":true}{"@context":"https://schema.org","@type":"BreadcrumbList","itemListElement":[{"@type":"ListItem","position":1,"item":{"@id":"https://www.gracelandfairlawn.com/","name":"Home"}},{"@type":"ListItem","position":2,"item":{"@id":"https://www.gracelandfairlawn.com/obituaries/","name":"Obituaries"}},{"@type":"ListItem","position":3,"item":{"@id":"https://www.gracelandfairlawn.com/obituaries/david-barr-18","name":"David C Barr"}},{"@type":"ListItem","position":4,"item":{"@id":"https://www.gracelandfairlawn.com/obituaries/david-barr-18/obituary","name":"Obituary"}}]}David C on Tuesday (03-11-2025) with his family by his side He started his career at OK’s Transmission and then worked and retired from Caterpillar after many years He was a great mechanic and tried to help as many people as he could Dave enjoyed riding his Harley Davidson and was always on the go He loved being with family and friends teasing and joking around He is survived by his wife Gale of 55 years; children: David C Dave was preceded in death by his father Robert “Bob” Gilead Cemetery following the funeral service Condolences may be shared with the family at www.gracelandfairlawn.com Share a story where David's kindness touched your heart Describe a day with David you’ll never forget Photo/VideoCemetery DetailsMt. Gilead CemeteryMt. Gilead Rd., Decatur IL 62521 This story has been updated to add new information — The field of candidates in the upcoming race to replace outgoing U.S a Republican who's represented the Lexington area for a dozen years announced he will be running for McConnell's Senate seat next year He launched his campaign April 22 with a video posted on social media taking aim at the "woke left" and promising to support President Donald Trump Barr made an in-person campaign announcement during an event in Richmond McConnell announced in February that he would not be seeking an eighth term in the Senate ending his run as the longest-serving senator in Kentucky's history Barr has represented Kentucky's 6th Congressional District since 2013 and has continually risen in the ranks as a federal lawmaker. He was a frontrunner to chair the House Financial Services Committee this year, and currently chairs the House Financial Institutions Subcommittee he was a legislative assistant for former U.S and served as deputy general counsel for former Kentucky Gov saying he's running for Senate to "help our president save this great country." Priorities he lists in the video include cutting taxes firing "deep state bureaucrats who steal our freedoms," deporting undocumented migrants in the U.S promoting coal and ensuring transgender women are required to use restrooms and other facilities aligned with their biological sex whine and blame Trump — they always do," he says the sun shines bright on our old Kentucky home the Louisiana Republican who serves as House majority leader a New York Republican and notable Trump ally who called Barr "as reliable as they come in fighting for President Trump and supporting our MAGA agenda." Riley Gaines, a former University of Kentucky competitive swimmer and anti-transgender activist, also endorsed Barr, emphasizing in a social media post that Barr has "fought nonstop to protect girls' sports." Gaines previously supported Daniel Cameron in the 2023 gubernatorial election and joined him on his campaign trail Cameron is also running for McConnell's Senate seat who sent a statement saying the longtime Southeastern Kentucky congressman has "no doubt that Andy Barr will fight every day to protect Eastern Kentucky and Kentucky Coal in the Senate." turned out for his campaign kickoff in Richmond Barr made his message clear: Trump needs more "America first fighters," and he wants to be one of them in the Senate "I'm running for the Senate with everything I've got to keep on fighting to make America great again," Barr said "I truly believe that President Trump's life was spared in Pennsylvania because God had a plan for him and we're watching that plan unfold every single day." He emphasized he would continue to work with Trump to embrace innovation and give Americans better access to "more affordable and innovative financial products and services" to increase the power of free enterprise and restore the "American dream." Barr also praised policies Trump has implemented including executive orders that protect coal-fired power plants and banning transgender athletes from girls and women's sports one of the strangest things I see right now is that the people who scream and cry about equality and fairness are the very same people who can't even define what a woman is and refuse to protect our daughters in girls sports," Barr said Barr told reporters "we're working every day to earn it." "We feel really good about where we are there we want to earn the support of the voters of Kentucky and they're the ones who are ultimately going to decide this election," Barr said He also touted his current role as a congressman saying he is in a unique position to help advance Trump's agenda now and fight for "America-first policies." Multiple speakers also spoke in support of Barr at the event including Marine Corps veteran Matt Bradford who said he "couldn’t think of a stronger person to stand up for all of us than Andy Barr." Pastor Rodney Coffey said while he's seen a lot of politicians change when they step foot in Washington "He stood for Kentucky values," Coffey said "He stood for the values that we stand up for He's never turned his back on his Christian convictions and that's why we need this man in the U.S Who else is running for McConnell's seat?Two other candidates have said they'll run for the seat and at least one more is likely to enter the race Cameron, the former Kentucky attorney general, entered the race in February, announcing his campaign minutes after McConnell said he would not seek reelection Cameron was the first Black attorney general in state history and would be the first Black senator from Kentucky including serving as the senator's legal counsel for about two years before he won the attorney general's race in 2019 Nate Morris, a chairman and chief executive officer of a privately held conglomerate in Lexington, is also considering a run as a Republican candidate for the seat but hasn't made an official announcement arguing they would serve as McConnell "puppets" if they win the office On the Democratic side, state Rep. Pamela Stevenson launched her campaign in April touting her deep ties to the commonwealth and background as a colonel in the U.S Stevenson ran for Kentucky attorney general in 2023 but lost to former U.S. Attorney Russell Coleman, who received about 58% of the vote She is the Democratic Party's House floor leader in Frankfort and is from the Shawnee neighborhood in Louisville's West End Stevenson would also be Kentucky's first Black U.S Barr has already drawn criticism from rivals and those who oppose his candidacy He shared a photo on April 17 of the invitation to Barr's campaign event arguing the congressman was "banning the 2nd Amendment at his campaign launch event" because weapons are not allowed on site And his campaign was critical following Barr's April 22 announcement "The last thing Kentucky needs is another career politician who’s going to vote like his 'mentor' Mitch McConnell and thwart President Trump’s America First Agenda," Morris spokesperson Conor McGuinness said a Republican who broke with President Donald Trump during her time in office before the general election later that year on Nov What does Barr's announcement mean for his district?Barr running for Senate means there's an open seat for Kentucky's 6th Congressional District The Democratic Congressional Campaign Committee is already targeting the district as one of 35 they hope to flip in the 2026 midterm elections. The district hasn't elected a Democrat since 2010, when they chose Democratic incumbent Ben Chandler While no one has announced an official campaign yet Ryan Dotson and Deanna Gordon are potential contenders for the Republican primary Kentucky Democratic Party Executive Director Morgan Eaves have been discussed as candidates in the Democratic primary Reach reporter Hannah Pinski at hpinski@courier-journal.com or follow her on X The holiday commemorates the Mexican victory in the Battle of Puebla in 1862 This year marks the 38th annual event put on by The Portland Guadalajara Sister City Association The celebration features more than 40 food vendors Brightly colored amusement rides will line the city’s waterfront all weekend The festival schedule is jam packed with events you can stroll the grounds to peruse the art and trinkets for sale The event prides itself on being a bilingual affair for the whole family Kids are encouraged to play one of the many carnival games for the chance to win a large stuffed animal Adults can purchase a wide variety of margaritas from the alcohol booths You can join in on the celebrations Friday May 2-Sunday May 4 2025 from 11 a.m.-11 p.m. and Monday May 5 from 11 a.m.-10 p.m at Tom McCall Waterfront Tickets start at $11 for kids and $16 for adults This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page Receive emails when new obituariesare published to our website Top hats were in fashion and people connected through telegrams rather than texts And the need for family and friends to come together Planning your funeral in advance means your family won’t have to when the time comes >>LEARN MORE Services that honor the individual and provide peace of mind for families >>SEE OUR SERVICES Unparalleled care at every step with unique and personalized memorial services >>SEE OUR SERVICES Chapman Funerals & Cremations - Bridgewater Chapman Funerals & Cremations - East Bridgewater Chapman Funerals & Cremations - Falmouth Chapman Funerals & Cremations - Harwich Funeral Home Chapman Funerals & Cremations - Harwich Chapman Funerals & Cremations - Marstons Mills Chapman Funerals & Cremations - Martha’s Vineyard Chapman Funerals & Cremations - Mashpee Chapman Funerals & Cremations - Wareham Chapman Funerals & Cremations - West Falmouth Funeral Home Chapman Funerals & Cremations - West Falmouth Chapman Funerals & Cremations - Yarmouth © Chapman Funerals & CremationsSupported by SRS Computing Your browser may not work with certain site. Upgrade now. rewritten or redistributed without permission https://www.northsidesun.com/peter-barr-miazza Click here for additional copyright information about this article Local news coverage is critical for a strong and vibrant community local publications are dying because of monopolistic Big Tech Over 30% of all newspapers are expected to close within the next three years Help support local news by making a donation today You would be supporting a local institution founded upon principles of integrity and strong editorial standards and have roots embedded in our local community Realtime Radar New rainfall amounts between a tenth and quarter of an inch possible Hourly Forecast 7-Day Forecast Based on data from National Weather Service and OpenWeather If you are an existing Print subscriber click HERE for your login info you can also easily register or login with an existing Google you can also easily use an existing Google User Help/Support Page Copyright Email: admin@northsidesun.com © Copyright 2025 The Northside Sun, 246 Briarwood Dr.- Jackson, MS 39206  | Terms of Use | Privacy Policy Apr 25, 2025Roseanne Barr sent a bold message about The Conners series finale after she was written off the reboot in 2018 On April 24, Barr's son and rep, Jake Pentland, issued a statement to TMZ about the show ending 🎬 SIGN UP for Parade's Daily newsletter to get the latest pop culture news & celebrity interviews delivered right to your inbox 🎬  and her family were "still glad" The Conners cast "got seven solid seasons of work out of the show she built." he said they "didn’t watch a single second" of the reboot after Barr was forced out She and the family also "didn’t even know it just wrapped." Barr played the titular character in the original series, Roseanne she joined the show's reboot The Conners but was ultimately written off following controversial tweets She later issued a public apology However, ABC condemned the message as "abhorrent repugnant and inconsistent with our values" and announced Barr's exit from The Conners Next: Roseanne Barr Details Feeling Like Former Costar "Destroyed" Her Life the program helps students develop effective oratory skills that allow them to stand out in a professional setting.  Ten honors students elected to join the semester-long course and become inaugural Barr Fellows honing their presentation and storytelling skills with the mentorship of Neighbors and their peers.  Hybridization is a process that allows plants to rapidly evolve by transferring beneficial traits from well adapted species to poorly adapted species.   “If you’re interested in creating a big drought-resistant tomato you could cross a big tomato with a drought-resistant one and maintain most of the big tomato genes with just enough of the drought resistant ones,” Cunningham explained.   By studying this process in milkweed Cunningham revealed how plants and food systems can evolve in the face of climate change.   © 2025 Advance Local Media LLC. All rights reserved (About Us) except with the prior written permission of Advance Local Community Rules apply to all content you upload or otherwise submit to this site YouTube's privacy policy is available here and YouTube's terms of service is available here Ad Choices Crohn’s disease including bacterial changes in the microbiome that foster an inflammatory environment Crohn’s disease been tied to a virus — specifically best known for causing infectious mononucleosis (mono) Researchers had already observed increased levels of EBV in the intestine of patients with Crohn’s disease and had also found associations between EBV and autoimmune diseases other than Crohn’s ‘It was a classic chicken and egg problem,” says Anubhab Nandy, PhD, a research fellow in the Division of Gastroenterology, Hepatology, and Nutrition at Boston Children’s Hospital A longitudinal study just published in Gastroenterology systematically looked at the role of viruses in Crohn’s and found strong evidence that EBV infection predisposes people to develop Crohn’s Nandy and colleagues analyzed data from a cohort of initially healthy military recruits who provided periodic serum samples throughout their service a high-throughput assay developed by study coauthor Stephen Elledge VirScan enables detection of antibodies against a wide range of viruses military personnel whose samples were positive for anti-EBV antibodies were three times more likely to eventually develop Crohn’s disease evidence of EBV exposure preceded their Crohn’s diagnosis by five to seven years we looked very specifically at immune responses to EBV with more detailed tests.” These tests bore out and strengthened the VirScan findings The team next looked for anti-EBV antibodies in a second cohort — more than 5,000 children (median age 11 years) who were first-degree relatives of people with Crohn’s disease EBV was not a statistically significant predictor of a subsequent Crohn’s disease diagnosis Snapper speculates that having first-degree relatives with Crohn’s could already have put them at increased risk due to shared genetic or environmental factors Another possibility is that EBV affects children’s immune systems differently perhaps because children are less likely to develop infectious mono when exposed “Responding to certain organisms early in life may boost the immune system in a way that prevents immune-mediated disease,” Snapper says Nandy and Snapper now want to figure out what EBV does to people at a molecular level to make them more susceptible to Crohn’s disease One possibility is that the virus has certain genes or molecules that interact with human genes involved in susceptibility to autoimmune conditions Another may relate to an anti-inflammatory protein produced by EBV People exposed to that protein may make antibodies against it preventing their own IL-10 from working and leaving them susceptible to inflammatory disease we need to understand exactly how EBV alters the immune system leading to Crohn’s disease,” Snapper says Learn more about the Inflammatory Bowel Disease Center at Boston Children’s Sign up for our weekly email newsletter for the latest parenting tips and news for your family from Boston Children's To make your experience more customized and user-friendly we use cookies for functional, analytic, and user-experience purposes. We do not share your confidential medical information with third parties using these tools. You can learn more by reading our Privacy Policy and our Terms of Use We use cookies to help you navigate efficiently and perform certain functions You will find detailed information about all cookies under each consent category below The cookies that are categorised as "Necessary" are stored on your browser as they are essential for enabling the basic functionalities of the site We also use third-party cookies that help us analyse how you use this website and provide the content and advertisements that are relevant to you These cookies will only be stored in your browser with your prior consent You can choose to enable or disable some or all of these cookies but disabling some of them may affect your browsing experience Necessary cookies are required to enable the basic features of this site such as providing secure log-in or adjusting your consent preferences These cookies do not store any personally identifiable data Functional cookies help perform certain functionalities like sharing the content of the website on social media platforms Analytical cookies are used to understand how visitors interact with the website These cookies help provide information on metrics such as the number of visitors Performance cookies are used to understand and analyse the key performance indexes of the website which helps in delivering a better user experience for the visitors Advertisement cookies are used to provide visitors with customised advertisements based on the pages you visited previously and to analyse the effectiveness of the ad campaigns We have the address for the funeral home & the family on file If you're not happy with your card we'll send a replacement or refund your money The family of Stuart Barr created this Life Tributes page to make it easy to share your memories passed away peacefully surrounded by her family She was the cherished wife of Allen O'Neal Smith for 62 years a testament to their enduring love and devotion Carol was a devoted mother to three daughters Her life was richly blessed by her grandchildren She also took great joy in her great-grandchildren each of whom brought a special light to her life Valree Wooten (Doug) and Charleen Fitzgerald (Joe); brother Steve Wadford; and many nieces and nephews Edward and Arlistene Wadford; her dear daughter Carol's sense of humor and deep love for her family will forever be remembered but her memory will live on in the hearts of all who knew and loved her A service to celebrate Carol's life will be held at Southland Memorial Gardens on Friday The family will receive friends prior to the service at 10:00 am to 10:45 am at Barr-Price Funeral Home A.G. BARR p.l.c. (LON:BAG - Get Free Report)'s share price hit a new 52-week high during trading on Thursday The company traded as high as GBX 703 ($9.34) and last traded at GBX 701 ($9.32) The stock had previously closed at GBX 687 ($9.13) Shore Capital restated a "house stock" rating on shares of A.G Read Our Latest Analysis on BAG The company has a fifty day simple moving average of GBX 638.80 and a two-hundred day simple moving average of GBX 622.49 a current ratio of 1.87 and a debt-to-equity ratio of 2.62 The stock has a market capitalization of £767.68 million a price-to-earnings-growth ratio of 1.36 and a beta of 0.38 A.G. BARR (LON:BAG - Get Free Report) last released its quarterly earnings results on Tuesday The company reported GBX 39.77 ($0.53) EPS for the quarter BARR had a return on equity of 12.27% and a net margin of 8.81% will post 43.7109104 earnings per share for the current year The business also recently disclosed a dividend May 8th will be issued a dividend of GBX 44.80 ($0.60) per share BARR's dividend payout ratio (DPR) is presently 49.20% insider Euan Sutherland purchased 11,990 shares of the firm's stock in a transaction that occurred on Monday The shares were bought at an average price of GBX 675 ($8.97) per share for a total transaction of £80,932.50 ($107,580.09) Insiders acquired 12,063 shares of company stock valued at $8,138,446 in the last ninety days 23.13% of the stock is currently owned by corporate insiders Which stocks are likely to thrive in today's challenging market Enter your email address and we'll send you MarketBeat's list of ten stocks that will drive in any economic environment Daniel “Luke” Taylor was called to his Heavenly home on Tuesday after a courageous battle with Duchenne Muscular Dystrophy Luke was a blessing to everyone who ever met him South Carolina to become a member of the Taylor family Luke was passionate about three things: His Love for God His Love for His Family and His Love for Sports.  He was a little man but he had a big heart.   Luther and Wanda Taylor of Lexington; three sisters Philip Taylor all of Lexington.  He is also survived by nephews Taylor Miles.  He is survived by one sister Romania and a nephew in Romania.  Additionally Luke is predeceased by his paternal grandparents and Kathleen Taylor; maternal grandparents Funeral service will be held 3:00 pm Saturday Visitation will be prior to the service at the church from 1:00-2:45 p.m.  Pallbearers will be Robert Boozer The family would like that those who attend the service either wear Carolina Gamecocks or Atlanta Braves shirts or their team colors.  In lieu of flowers the family request you send memorials to First Calvary Baptist Church or Connie Maxwell Children’s Home Honorary pallbearers are Jack Herring’s Sunday School Class. Barr-Price Funeral Home and Crematorium Please select what you would like included for printing: Copy the text below and then paste that into your favorite email application He grew up with his four siblings in Wisconsin and spent the majority of his childhood in New Richmond His fondest childhood memories included fishing He was a multi-sport athlete in high school and participated in football Craig enlisted in the US Air Force in 1974 and served as an E4 Senior Airman until 1977 at Offutt Air Force Base Craig went on to complete his bachelor’s degree at the University of Nebraska Omaha He then enrolled in medical school at the University of Wisconsin-Madison where he earned his doctorate in internal medicine graduating summa cum laude.  During Craig's internship at Gunderson Lutheran Hospital in LaCrosse The whirlwind romance began in October of 1983 Craig’s residency moved them to Marshfield Craig practiced medicine for a year in Sturgeon Bay He then accepted a position in Council Bluffs Craig thoroughly enjoyed serving his patients at Bluffs Family Healthcare He entered into early retirement due to a medical condition resulting in disability.  His favorite vacations with Kathy and the girls were to the Rocky Mountains of Colorado and Door County and he held a particularly special place in his heart for his last cat His favorite holiday was the 4th of July and his fireworks always stole the show He was an avid football fan and loved cheering on his Green Bay Packers and Wisconsin Badgers  Craig’s three girls meant the world to him he found a new joy in being known as grandpa He treasured his time with visits from his kids and grandkids He often joked that the grandkids were always welcome as long as the visits weren’t too long He looked forward to his frequent facetime calls with his kids and grandkids as well  Craig was preceded in death by his parents brothers Terry (Irina) Barr and Brian (Jill) Barr  We would love for you to join us in remembering and celebrating his life Visitation will be from 3:30-4:30pm at Hoy Kilnoski Funeral Home on Saturday Memorial Service with military honors will follow at 4:30pm Craig will always be remembered for his remarkable intelligence steadfast devotion to his patients throughout his medical practice his memory and legacy will live on in the hearts of all those who knew and loved him Enter your phone number above to have directions sent via text This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply Service map data © OpenStreetMap contributors R-Lexington setting his sights on the Senate there will be a new face representing Kentucky’s sixth district next year. The primaries are over a year away but some people are already preparing to replace Barr Since 2013, Lexington and central Kentucky have been represented in Washington by Barr, but next year that will change. Last Tuesday, Barr announced he hopes to replace long time Senator Mitch McConnell R-Winchester declared his candidacy for the seat He said he decided after praying with his wife “We had been looking at this scenario playing out for the last year or so,” Dotson said Dotson announced his candidacy for Barr’s seat last Tuesday as well He said he will bring the fight to Washington I will be very vocal; I will be very active and I will push back against the ‘Woke Mob’ in D.C,” Dotson said Dotson has been in the Kentucky House since 2021. In 2022, he authored and helped pass a law banning transgender women from competing in women’s sports This has also been a major focus of President Donald Trump’s agenda “I do plan on working on that and helping Donald Trump fulfill that agenda nationally as well,” Dotson said said a run for Congress is under consideration I am a listener for the people and someone who will support and fight for the Trump agenda,” Gordon said Gordon has been in the House since 2019 and is a lifelong resident of Richmond She’s an audiologist and businesswoman and was recently named chair of the House small business committee.  “The thing that I’m most proud of is lowering our personal income tax We’re on the march to zero [percent] and this past term we lowered it to 3.5% and I would love to do those things on the national level,” Gordon said Dotson is a businessman and has two children and multiple grandchildren He said while he’s outspoken about social issues he also hopes to expand economic investments into central Kentucky and reduce the cost of living “We need to really cut wasteful spending in Washington D.C and therefore being able to lower the taxes upon our citizens because cost of living has gotten so high,” Dotson said “We need to find ways to make it easier for working class families.” Other names to watch entering the race include State Senator Amanda Mays Bledsoe Former Republican State Senator Damon Thayer said he will keep all options open the Democratic Congressional Campaign Committee and third district Rep are hoping to flip the seat in the 2026 election Spectrum News 1 spoke with former democratic State Rep who said she is still discussing whether to enter the race Barr created this Life Tributes page to make it easy to share your memories You can reach us 24/7/365 at (302) 731-5459 Copyright © 2025 Strano & Feeley Family Funeral Home and Crematory