It's one of our favorite celebrations, bringing everyone together, young and old, rockers and classical music fans alike. The Fête de la Musique is back on Saturday sing and have fun to the rhythm of free concerts organized all over France In Gif-sur-Yvette via the Levasseur park and the various districts there will be concerts and entertainment for all ages and tastes while the musical evening at Parc Levasseur is scheduled for Saturday June 22 2025 - 20:30 ⤏ 23:59Café le Belleville and its friendly team welcome the group "Maizena Ping Pong Club" Music runs, music runsSaturday, June 21, 2025 - 18:00 ⤏ 23:59* The seed https://www.instagram.com/la_graine_pr?igsh=dHZ6aW9mYWU0MGNr * Amalgammehttps://jazzy-stems.wixsite.com/amalgamme * Dna Failurehttps://www.facebook.com/DNAfailure/ A suspended moment at L'île o' crêpesSaturday, June 21, 2025 - 19:30 ⤏ 22:30Les Darons , a charming duo, are back with their guitar-voice repertoire for your listening pleasure[https](https://www.instagram.com/lesdaronsmusic Les Darons (@lesdaronsmusic) - Instagram photos and videos) The Chevry plateau comes alive with summer colorsSaturday come and meet the "Nanafuvilo" and dance to country music or sway to rock standards; https://outlook.office.com/84014c2c-d635-4a85-8af7-071498325054 The sound of guitars soars at Le MoulonSaturday 2025 - 18:30 ⤏ 23:59Under the baton of Brass&co and the shopkeepers 4 groups will liven up the potter's mall * El Turista - acoustic rockhttps://drive.google.com/file/d/176AtH3mbP2EJhOxDIMhkpc1TPpAi8vGB/view?usp=drive_web * Intrasystoles pop rock- soul -jazzyhttps://youtu.be/bHy9bDGTqQQ?si=WFE4UZNMUYCi8dNP * Blister Feat Bandhttps://www.youtube.com/@TheBlisterFeatBand a jazzy universe for the start of the evening; then the temperature rises with Pawer and Néodyme in a more rock register to keep you dancing until the end of the night https:// youtu.be/B2S02BMOflg?feature=shared https:// neodyme-rock.fr/ https://orchestre-pawer.com/ Journey to sunny Madagascar to start the evening at Jo'SSaturday, June 21, 2025 - 19:00 ⤏ 20:30Ifara, Malagasy duo https://www.ifara99.wordpress.com Smalljump: Let yourself be carried away by this duo who will offer you a varied menu at the Jo's Terrace s@smalljump.music 2025 - 19:00 ⤏ 23:00Whether on the terrace of the Gif bistro or on the castle forecourt come and discover the new stage facing the Val Fleury castle and hum along to its variety tunes with the bands Lost'in soul and Nanafuvilo Lost in soul : https://youtu.be/q5e9SrIZuF8?si=_npKQrRzneRuLtrm Nanafuvilo:https://outlook.office.com/84014c2c-d635-4a85-8af7-071498325054 A wonderful musical evening awaits you in this Essonne town Refer your establishment, click herePromote your event, click here Every summer, July 14th is a not-to-be-missed date for the French, who take advantage of the traditional military parade on the Champs-Elysées, fireworks displays in almost every town and city in France and family activities to explain the importance of this holiday to the youngest In theEssonne department, the town of Gif-sur-Yvette is also organizing its unmissable fireworks display on Saturday, July 13, 2024: come and watch them in the town hall park don't miss the dancing ball and the Republican picnic you're sure to have a great time on July 13 ShareSaveLifestyleTravelHow To Rent An Amazing, Affordable Vacation Property Once Owned By A KingByCatherine Sabino The Moulin de la Tuilerie in Gif-sur Yvette a town in the countryside outside of Paris or the equivalent to £22.19 per person per night at full occupancy For a smaller house party of three couples Le Moulin de la Tuilerie in Gif-sur-Yvette about 15 miles southwest of the French capital was the only home the Duke and Duchess owned together When the Windsors settled in France after World War II they rented a grand villa in Paris in the Bois de Boulogne decorated in suitably regal fashion with the help of Stephen Boudin the interior designer who headed up Maison Jansen whose  elite roster of clients included Jacqueline Kennedy with whom he collaborated on the refurbishment of the While House The Duke's bedroom at Le Moulin de la Tuilerie in 1952 to serve as a weekend escape from their worldly city life But while the country retreat was far more informal in style than their villa their guest list remained as posh as it was in Paris Maria Callas and Cecil Beaton stopping by for some R&R in this peaceful Le Moulin de la Tuilerie consists of three buildings—the two-story stone mill house and two guest cottages The Duchess decorated the main house (with input from Boudin) with colorful fabrics and carpets and spent years checking out the antique shops in Paris to find the right mix of pieces for the home including the Duke and Duchess’s former bedrooms Although little of the decor from the Windsors’ time remains in the house—the property had several owners after the Duke died—one piece from the period stands out a mural with an observation from the duchess but I have been through the mill." The house decor today is a mix of Anglo and French style Les Maison des Amis rents for 4 nights from £530 or £33.13 per person per night; La Célibataire 4 nights from £311 or £38.88 per person per night For more information go to: Landmark Trust The dates displayed for an article provide information on when various publication milestones were reached at the journal that has published the article activities on preceding journals at which the article was previously under consideration are not shown (for instance submission All content on this site: Copyright © 2025 Elsevier B.V. In tune with the ethics promoted by the brand Danone through the motto “One Planet One Health,” the studio Arte Charpentier has created the research center In’Cube with an eye on sustainability and mixed functions Client: Danone Nutricia ResearchArchitecte mandataire missions conception et exécution: Arte CharpentierArchitecte associé: Abbès TahirHQE Bâtiment Durable 2016: Niveau ExcellentCertification BREEAM NC 2016: Niveau ExcellentLabel OSMOZ: 1 levierLabel énergétique BBCA V3: Niveau StandardPrix: Grand Prix du Cadre de Vie 2022Photo credits: Christophe Valtin a synthesis of the needs expressed by Danone and a welcome anticipation of the post-Covid workplace The center embodies the concept of the functional mix through different spaces for distinct uses and contributes through its architecture to create a feeling of belonging to the brand on the part of employees encouraging exchange and interaction.” This is how Abbès Tahir explains the key concepts approached in the creation of the new international center Danone Nutricia Research the innovation and research branch of Danone Group The physical context is that of Gif-sur-Yvette in the Paris-Saclay Science Center a campus that safeguards about 30 small towns about 320,000 inhabitants and 60% agricultural and natural spaces while hosting one of the world’s eight most important clusters of technological research The present facilities for pharmaceutical and university research will be joined in the years to come by the conservation and creation division of Centre Pompidou which will also contain the storerooms of the Musée Picasso-Paris The context for the project is very complex a crossing between an office building and an industrial complex welcoming over 600 people and responding with a low-carbon approach to the values of environmental sustainability espoused by the company of guiding the future nutritional revolution also in collaboration with specialized start-ups The project has to apply the best ergonomic design respecting parameters of regulation and maximum privacy while guaranteeing the coexistence of internal activities of production and research with the presence of external visitors invited to the facility to test new food products reuse and the circular economy become indispensable conditions for an eco-responsible and creative project which has received many sustainable energy certifications 21,500 sqm) utilizes four levels to organize an industrial area zones for creative workers and food prototyping pilot workshops and scientific laboratories coworking facilities with offices and meeting rooms the base has been made in concrete with low carbon emissions while from the second floor up the structure becomes lighter thanks to the use of wood that sets the entire character of the core of the design: a large central atrium with zigzag roofing which offers splendid views of the surrounding landscape and a sequence of terraces open to the city the lobby and event space catalyzes pathways encouraging encounters and setting the pace of life for all those who use the center Arte Charpentier have paid close attention to aspects of landscape and environment not only through optimization of hydric resources but also with about 3000 square meters of plantings in keeping with an extensive concept of biodiversity attenuates the landscape impact of the roofing while other green spaces on the ground are set aside for employee relaxation These include the “rain garden,” a slightly sloping space which in the rainy season functions for the gathering of rainwater The largest diffusion magazine in the luxury & design world Text description provided by the architects. LAN, in association with CVA and the landscape architect Topotek, has just completed plot B, a highlight of the Grand Paris project, for a total of 900 residences, serving 1,082 students. Spanning two Urban Development Zones, the École Polytechnique district and the Moulon district, this large-scale campus represents the pinnacle of national higher education and research, and is one of the greatest student residences ever created in France. While the buildings along the perimeter of the plot cultivate an urban lifestyle the residences in the “Muses” form a more innovative habitat The circular forms allow the façade length per residence to be increased while reducing the façade length for the total surface area The cylindrical plan optimises the distribution surfaces reducing the surface area of the entry and allowing spaces for separate living and sleeping areas The vertical circulations are established at the centre of the layout connecting to an elevator and an enclosed stairway serving 14 residences per level with an average surface area of 18.50 m2 each The peripheral buildings offer various types of housing ranging from the T1 clusters which surround common living areas You'll now receive updates based on what you follow Personalize your stream and start following your favorite authors If you have done all of this and still can't find the email The Escherichia coli bacterium is a major focus of research in biology But the higher-order organization of its genome had yet to be investigated using techniques such as chromosome conformation capture (3C/Hi-C) scientists from the Institut Pasteur revealed using this approach the existence of several levels of chromosomal folding linked to known proteins including condensin and the histon-like HU protein whose roles in maintaining the structure of this genome were until now unclear "All genomes must be specifically organized to ensure they function correctly for instance to regulate the access to the genetic information they contain Head of the Spatial Regulation of Genomes Unit at the Institut Pasteur which are generally made up of a single circular chromosome are no exception." This chromosome is organized in a structure called a nucleoid which at the same time enables gene regulation and the segregation of replicated chromosomes The findings of previous research carried out on different bacterial species showed that nucleoid formation is a result of different processes: By using different approaches combining genomic methods (chromosome conformation capture or 3C/Hi-C) genetic methods and fluorescence microscopy the Collège de France and Pierre and Marie Curie University studied the impact of several factors controlling chromosome folding and multiscale organization in the model bacterium Escherichia coli By analyzing the organization of chromosomes from different E which quantifies the physical contacts between segments along DNA molecules the scientists were able to determine the influence of transcription regulation on the local DNA structure and the roles of the main proteins associated with the nucleoid on the 3D organization of the chromosome "We have identified two methods of communication in the chromosomal DNA which define two separate structural entities – the ter region the part of the chromosome where genome replication ends which is consistent in the main part of the chromosome These contacts are the result of the combined action of the bacterial condensin and HU protein While it is not surprising that condensin activity stimulates contacts between distant DNA sites a role for the ubiquitous proteins HU in promoting such long-distance interactions in vivo had never previously been described The second method of communication regulates the ter region where a protein thus isolating this region from the rest of the genome MatP has a structure regulator role to play by preventing the condensin to promote contacts between distant sites in the ter region" and this challenges a model whereby this protein The research reveals multiscale chromosome conformation involving proteins with different and unexpected properties to control chromosome dynamics the findings highlight the unique properties of the ter region in E coli and show how this species has selected strategies that confer specific and distinct properties to this chromosomal region that are different for example from other bacteria such as Bacillus subtilis These key discoveries are vital for understanding bacterial chromosome metabolism They also show that the ubiquitous and very abundant DNA-binding proteins such as HU which have long been known to interact with DNA but whose roles remain unclear are important for the multiscale structuration of the chromosome in vivo which are widely used in eukaryotes but relatively seldom in bacteria demonstrate here their relevance for studying nucleoid function in which techniques such as transcriptomics epigenomics and metabolomics can be used to obtain tens of thousands of data points in a single analysis of a given sample such as a cell The current sequencing output can be as high as 60 billion nucleotides per day and biologists have begun analyzing larger samples such as the microbial population in a drop of water a handful of earth or the intestines of a cohort of patients." From the book Institut Pasteur / Today's Research US$35.00 / CAN$44.00 / UK£26.99.All royalties donated to the Institut Pasteur Learn more © Fred DelangleThe Research area, designed by Groupe-6 architects, opens to the east of the atrium. The Rue de la Recherche hosts state-of-the-art laboratories, workspaces for researchers, and pleasant meeting places. The openings between floors visually connect the building and its four patios. The research street is open 24 hours and free to access, unlike laboratories whose entry is strictly controlled. The urban siting of the project determined two principles for the project’s facades. © Fred DelangleTo the north and along the main campus avenue, the architects chose a fully glazed façade—a “vitrine” capable of showcasing activities of exchange and encounter without requiring significant protection from sunlight. To the south, east, and west, the white precast concrete ribs articulate the building’s mass. The verticality of the structural concrete is echoed in the large glass surfaces of the windows punctuating the white facades and reflecting the surrounding landscape. © Christian RichtersThe intentionally sober expression of glass, light, and white surfaces is offset by perforated acoustic OSB wood panels that are used throughout the project to deliver visual warmth through a low-cost, sustainable material. Internal gardens are dispersed throughout the complex at ground level, and the external landscaping features Apothecary Gardens planted with medicinal herbs and grasses. © Fred DelangleThe complex is linked to the University’s energy grid via geothermal wells and has been certified as conforming to France’s High-Quality Environmental standard of green building with an advanced level of energy efficiency named after the Nobel Prize-winning French chemist and pharmacist Planning and construction were led by Bouygues Construction as part of a public-private partnership following a two-and-a-half-year international competition If you like to run while having fun, then don't miss the Color Run in the town of Gif-sur-Yvette, on Saturday June 17, 2023! Stemming from the world's most colorful sporting event, this fun run is becoming increasingly popular with the aim of crossing several color zones and crossing the finish line with as many shades as possible on you just having fun with family and friends on a festive day through the city streets From the age of 7, enjoy this colorful 5-kilometer race, organized by the Youth Council as part of "La Fête du sport - Terre de jeux 2024". For this occasion, the colors will be very special, there will be 5, those of Olympism:blue Each participant receives a "Color Run pack": a white Color Run T-shirt and colored sunglasses The race is free with registration but there are only 300 places available at the start Are you ready to see life in all its colors For the man who gave up the throne of England to be with the women he loved living in seclusion became a life sentence Exiled from England to avoid stirring up trouble for the Royal Family Edward and his American wife settled in Paris and lived in the beautiful Chateau Le Bois which became better known as “Villa Windsor” famous for the glamorous parties attended by the crème de la crème of café society was their much-loved and enchanting weekend home an 18th century mill at the mouth of the Chevreuse valley And I bet you wouldn’t believe me if I told you Le Moulin de la Tuilerie as it was named by the Duchess, could be your own countryside retreat for the price of budget hotel almost every weekend the Royal outcasts would leave town heading 20 miles southwest of the city; Edward in his Chevrolet They typically invited friends to join them– Maria Callas Cecil Beaton and various celebrities of the day were among the glittering guest list that passed through the gates of the Moulin at Gif-sur-Yvette.  It was the only house Wallis and Edward ever actually owned They bought the flour mill from artist Adrien Étienne a fashion illustrator whose work appeared in all the women’s style magazines of the 1920s & 30s before the days of photography and used the mill as an artist’s retreat where Picasso and Fernand Leger often came to stay but Edward became so attached to the charming property that he made it their own spending 50,000 Francs on a two-year renovation to transform it into the quintessential English country house in the middle of France For the homesick English ex-King that never was The Duke called on the great English garden designer Russel Page to design his beloved English garden which he maintained passionately with his staff of five gardeners A swimming pool was also installed with a changing hut with his-and-hers cubicles.  The outbuildings were transformed to accommodate their constant flow of guests The old pigeonnier (dovecote) became an outside dining room the large barn converted into “the Orangery” which served as the main living room and the stables became further bedrooms and a study The Moulin itself (the windmill) became a space for entertaining and dancing and also housed separate sleeping wings for the Duke and Duchess the interior designer who went on to work with Jackie Kennedy on the White House (More photos of the interiors here and here) After the Duke died in 1972, Wallis sold the home and it was owned briefly by a Swiss business man and then a Lebanese doctor before the Moulin went into a period of decline Ivy crawled into every room of the former Royal home and the garden that had been faithfully tended to by the Duke was swallowed by vegetation; real-life secret garden the home was rescued from dilapidation by a British property company hoping to give the buildings a new future as a holiday accommodation The restoration was a significant undertaking the Landmark Trust came on board to help with the renovation and furnishing of the retreat for its new purpose as an inspiring place to escape the city.  While little of the Windsor’s plush decoration remains such as Stéphane Boudin’s mosaic wall decoration in the sitting room of the Pigeonnier and the Dukes walled garden still grows behind the Lodge The lavish lifestyle of the exiled English royals has been swapped for simpler but the former home of Edward and Wallis still reveals its own stories of an enigmatic relationship The Landmark Trust calls it “an echo of Englishness recalling happy times” and rents out the 7 bedroom moulin for a very reasonable price. A four night stay, if you can fill the property, works out at £22.19 per person per night Just the right budget for kings and queens in exile Last Updated on August 17, 2021 by Pauline Harboe Text description provided by the architects. A house for researchers and research. The Campus Plateau de Paris-Saclay is the Silicon Valley of Europe. Dietmar Feichtinger Architectes, together with Celnikier & Grabli Architectes and Ingerop Conseil & Ingénierie, was in charge of the realization of the Institute for Neuroscience. The new four-story building provides optimal living, research, and working conditions for 350 scientists, 40 students, and the laboratory animals in the basement and on the ground floor. The triangular, single-story volume of the entrance area with auditorium and foyer comes out from the façade in the south as the institute's calling card. Three-story glass bridges between the cross-wings spacious staircases and terraces transform atriums and access zones into places of encounter The institute went into operation in March 2020 The main street cuts diagonally across Lab City allowing a seamless experience between the building and its surroundings providing a convenient public route to the neighbourhood and the future subway station The architecture provides a framework for constantly changing requirements allowing the programme to be reconfigured or intensified as necessary OMA’s multi-layered design integrates urbanism within the school The architects imagined the laboratories as a collection of discreet parcels in an open-plan grid The project features wide internal stairways A lightweight roof covers the entire complex allowing ‘external’ protected spaces to be used freely all year long For more information, vistit the OMA website Receive our daily digest of inspiration, escapism and design stories from around the world direct to your inbox. Text description provided by the architects. Situated on the Plateau de Saclay, a science cluster of international renown, the new Research & Innovation Centre of the Danone Group, certified as a low-carbon building, optimizes the quality of working life and permits the development, testing, and cultivation of future innovations. © Christophe ValtinThe architecture of the building provides transparency from the public space and allows the whole industrial process, the micro-factory, and the activities that take place there to be discovered. Standing 24 m high, In’Cube fits perfectly into its rectangular base of 90m long and 75 m wide, its particularitý lies in its U shape deployed around a luminous Plaza topped with a shed roof providing zenithal lighting and where wood is omnipresent. © Christophe ValtinThe overhanging plaza space offers exceptional views with its panoramic views of the surrounding landscape This central atrium promotes meetings and takes up the codes of a village square, which comes to punctuate the life of the community around different events, vertical circulations, and corridors surrounding and animate its volume and distribute the different premises. including a 1,100m² garden terrace with an orchard and You don't have permission to access the page you requested What is this page?The website you are visiting is protected.For security reasons this page cannot be displayed Metrics details Whether you are reading in Chinese or French Prices may be subject to local taxes which are calculated during checkout Nakamura, K. et al. Proc. Natl Acad. Sci. USA http://dx.doi.org/10.1073/pnas.1217749109 (2012) Vinckier F (2005) The neural code for written words: A proposal Schneider W (2005) Cross-cultural effect on the brain revisited: Universal structures plus writing system variation (2009) The graphemic/motor frontal area Exner’s area revisited Fox PT (2005) Neuroanatomical correlates of phonological processing of Chinese characters and alphabetic words: A meta-analysis Download references Reprints and permissions Download citation DOI: https://doi.org/10.1038/nature.2012.11883 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing newsletter — what matters in science The old mill house – and the surrounding cottages that together form Le Moulin de la Tuilerie – were the weekend retreat of the Duke and Duchess of Windsor In those days it was half an hour's drive from their main house in the Bois de Boulogne while none of the duchess's hectic decorative schemes survives the Landmark Trust – which specialises in caring for endangered historic buildings and letting them as holiday homes – has ensured that it is difficult to forget them The faces of the former king and his spouse leer in the form of blown-up framed photographs the sweet little guest cottage in which the artist photographer and wit Cecil Beaton is said to have stayed when he visited the couple When my boyfriend disappeared into the downstairs bathroom "This is definitely Cecil Beaton's hip-bath!" But then there was silence "There's even a picture of them above the lavatory," he said "Must we be confronted with the worst excesses of Britain's constitutional arrangement from every wall Perhaps we could drape a tea towel over them Photograph: Alastair MillerThough the Landmark Trust cares for many historic houses in Britain – and indeed has an apartment to rent on the Spanish Steps in Rome – Le Moulin de la Tuilerie is the first property it has taken on in France further buildings in Britanny and near La Rochelle (the latter property is a 19th-century fort built as a defence against the British) Le Moulin de la Tuilerie is on the edge of a little town called Gif-sur-Yvette Gif lies towards the end of the RER commuter line into the capital is that the artist Fernand Léger lived here – he was still alive residing in a handsome village house down the road when the Windsors bought the Moulin from the chic French illustrator Drian History does not relate what the elderly cubist painter thought of the exiled former Edward VIII and his wife Gif is neither quite suburbia nor quite deep countryside – commuter-belt Surrey comes to mind – though when we took a walk in the Bois d'Aigrefoin to the west of the mill a pair of deer leapt and darted in front of us and at night we heard owls from our roomy bedroom in the eaves The Moulin itself is a delight: a cluster of mostly 18th-century buildings given the duke and duchess's well-known taste for the high life and the splendour of their Paris residence which had previously been inhabited by Charles de Gaulle though it sleeps two) is the smallest of the Landmark Trust's three holiday lets here: La Maison des Amis sleeps four and the main house running down to the little river Mérantaise is the skeleton of the garden that the great landscape designer Russell Page created with the duke for the chest-deep pool of blue delphiniums and pink stocks that the duke nurtured is long gone and he liked nothing better than getting on his tweeds and having a good dig bullying his guests into helping him lay a stone woodland path or screaming orders in German to his Alsatian gardeners but the family mother tongue was in excellent nick.) The Duke and Duchess of Windsor at Le Moulin de la Tuilerie Photograph: Patrick Lichfield/ Cond Nast Archive/CORBISOpening on to the lawn is a great barn of a room it was once where the duke and duchess foregathered with all their weekend guests and you couldn't move for dainty tables and chairs coffee tables made out of regimental drums and elaborate knick-knackery of every kind Photographs of the decor show that the pièce de résistance was a carpet migraine-inducing shade of swirling emerald bright colours," she told a newspaper of Le Moulin's décor "Every house should have a theme: then the decoration becomes something like a musical composition; each room carries the theme but with variations of mood and pace." Whatever the theme was it found its apogee in the carpet of the drawing room in the main house: vermilion with a thick tartan ribbon detail writhing across it like a python in its death throes That duchess worked a Schiaparelli frock and an expensive jewel with aplomb but she let herself down with the decoration of Le Moulin (May I reassure you that the Landmark Trust has decorated it in unexceptionable sober taste – with the exception of those photographs.) The fact that the town of Gif is perfectly ordinary has its advantages between the mairie and the railway station from oysters and John Dory to ceps and those gloriously evil-looking purplish fungi "It's the only market in France not full of braying English people," said my boyfriend Those with a car might try undertaking some proper sightseeing in Chartres or Versailles we debated walking the 25 minutes to the RER station on the other side of Gif and taking the train to the end of the line where and 45 minutes after hopping on it were sauntering happily around the Luxembourg Gardens in central Paris The RER also takes you direct to the Gare du Nord so the trip is fantastically easy and quick by Eurostar: with a sharp but not punishing early-morning start we were back in London by 11.30am on Monday after our three-night weekend stay The canny weekender might consider a Friday-morning train to Gare du Nord stashing bags in left luggage and lunching in Paris before taking the RER in the late afternoon out to Gif The Windsors also let themselves down badly when it came to putting the property on the market in the late 1960s they proposed a scheme on the land for 537 dwellings and pulled every government string they could to see it accepted the good mayor of Gif stood up against them (vive la république!) and the scheme never came to fruition Even Diana Mosley – who lived a couple of stops up the RER line in Orsay in a gorgeous little Revolution-era building called Le Temple de la Gloire – thought this was poor form The Landmark Trust (whose patron is the Windsors' great-nephew Prince Charles) is an infinitely more reliable guardian of this pretty plot than the duke and duchess Charlotte Higgins is the Guardian's chief arts writer TheNicolas Tarkhoff exhibition at the Château du Val Fleury is a cultural event not to be missed immerse yourself in the world of an artist who lends itself magnificently to this retrospective is a Russian painter who has captivated critics and audiences alike His vivid palette and lively gestures evoke the ardor of Vincent van Gogh and the madness of Maurice de Vlaminck while recalling the meticulousness of Claude Monet This free exhibition takes us through the different phases of his career from his vibrant scenes of Parisian life to his peaceful landscapes of the Vallée de Chevreuse At the heart of theexhibition are works reflecting Tarkhoff's stylistic diversity - divisionism fauvism - all testifying to his artistic quest We also see the evolution of a man who was attentive to his times The exhibition itinerary is designed to immerse you fully in the hectic atmosphere of Parisian life that Tarkhoff so skilfully captured The works on show demonstrate his ability to capture "the life of forms through the richness of their colors" a quote that perfectly sums up his artistic approach This is a unique opportunity to see up close these vibrant canvases offers a historic and elegant setting for this exhibition you'll find yourself transported to another time and place sharing an intimate moment with art and history This free exhibition is an exceptional opportunity for art and history lovers but also for those wishing to discover an artist whose work deserves to be more widely recognized the Château du Val Fleury is the place to be for a journey through time and art You don't have permission to access the page you requested. What is this page?The website you are visiting is protected.For security reasons this page cannot be displayed. © Cyril WeinerThe central space is a versatile place capable of hosting workshops, conferences, and public events. Bathed in natural light thanks to a dome pierced at the zenith, it stages the activities of SIRTA and its relationship to the sky. Around this space, a large gallery serves all the research laboratories which are located in a third ring. Glazed on both sides, the workspaces offer tangent views towards the heart of the building and the surrounding nature.  © Cyril WeinerA concrete structure and an alternation of solid anodized aluminium panels and full height glazed panels make the building. The concrete is poured in one go. The rhythm is set by the concrete pillars, the aluminium thorns, and the steel guardrail of the roof. The exterior gallery which houses a covered circulation also protects the facade from rain and sun.  the outbuilding and parking spaces counterbalance the roundness of the observatory building and wedges the facility to the service road A landslide fueled by heavy rain caused three cars of a Paris-bound train to overturn Tuesday morning The accident happened between the southwestern suburbs of St-Remy-les-Chevreuse and Orsay about 5 a.m Michek Bournet, mayor of Gif-sur-Yvette, told La Parisien there were very few people on board at the time because it was the first train of the morning "We could have had a disaster because if it was an hour later there would have been a lot of students on board," he told the newspaper Bournet said the driver had been warned that a tree had fallen on the track but didn't see that part of the track had been washed away Officials couldn't say when the track would be reopened France has been dealing with days of heavy downpours. Several rainfall records were broken Monday and some locations received the equivalent of more than a month of rain in 24 hours according to the World Meteorological Organization At least four people were killed amid heavy downpours and subsequent flooding last week The heavy rain was caused by an area of low pressure in the upper atmosphere, according to weather.com meteorologist Chris Dolce. That area of low pressure is moving east, which should yield drier weather conditions during the second half of the week, though a few showers can't be ruled out We recognize our responsibility to use data and technology for good We may use or share your data with our data vendors The Weather Channel is the world's most accurate forecaster according to ForecastWatch, Global and Regional Weather Forecast Accuracy Overview Michek Bournet, mayor of Gif-sur-Yvette, told La Parisien there were very few people on board at the time because it was the first train of the morning \\\"We could have had a disaster because if it was an hour later there would have been a lot of students on board,\\\" he told the newspaper Bournet said the driver had been warned that a tree had fallen on the track, but didn't see that part of the track had been washed away Officials couldn't say when the track would be reopened France has been dealing with days of heavy downpours. Several rainfall records were broken Monday according to the World Meteorological Organization.  At least four people were killed amid heavy downpours and subsequent flooding last week.  The heavy rain was caused by an area of low pressure in the upper atmosphere, according to weather.com meteorologist Chris Dolce. That area of low pressure is moving east, which should yield drier weather conditions during the second half of the week, though a few showers can't be ruled out.  Metrics details Bacteria show sophisticated control of their cellular organization and many bacteria deploy different polar landmark proteins to organize the cell pole such as Photo-Activated Localization Microscopy (PALM) provides the nanoscale localization of molecules and is crucial for better understanding of organization and dynamics in single-molecule analytical tools are not fully available yet quantitative and statistical analyses of subcellular localization with multiple cells from multiple fields of view are lacking brightfield images are not sufficient to get accurate contours of small and low contrast bacterial cells compared to subpixel presentation of target molecules Here we describe a novel analytic tool for PALM which integrates precisely drawn cell outlines labelled by PALM-compatible fluorescent protein fusions with molecule data for >10,000 molecules from >100 cells by fitting each cell into an oval arc the polar anchor HubP constitutes a big polar complex which includes multiple proteins involved in chemotaxis and the flagellum HubP is shown to be slightly skewed towards the inner curvature side of the cell while its interaction partners showed rather loose polar localization Understanding the control mechanisms of polarity in space and time is now appreciated as a crucial question in bacterial cell biology there is a demand to develop means to transfer a PALM molecule list corresponding to protein locations within a cell with high-throughput capability we study the precise localization of polar proteins in V which combines PALM detected molecule lists with cell meshes which are drawn by MicrobeTracker We show that using brightfield (BF) images are not sufficient for precise localization analysis Therefore we present a novel cell outline technique in which the inner membrane or the periplasm is labelled with photo-activatable/switchable FPs We also show that Vibio can distinguish inner and outer curvature of curved-rod cells we show that HubP is rather localized to the inner curvature from the tip of pole while its interaction partners have distinct localization patterns This new labelling method and localization software will provide a better landscape of localization for single molecules in populations of cells (a,b) Representative image of cell with native level (a) or overexpressed (b) HubP-FPs conventional fluorescent image (ii) are also shown The region in the purple square is magnified in (iii) (c–f) Distribution of HubP clusters in native level expression (c and d) or overexpressed (e,f) conditions (c,e) Dot plots of number of molecules per cluster the cluster with highest number of molecules was indicated in red and other clusters were shown in blue The mean and standard error of mean are also indicated or 3 clusters of HubP molecules with respect to cell size 1.28 µm is the average cell size for these experiments HubP molecules accumulate into the existing cluster as well as form a new cluster at the new cell pole (discussed later) It is no wonder that a much higher total number of HubP-PAmCherry molecules were detected in overexpressing cells. Yet, cluster analysis indicated that HubP molecules are organized into only a single cluster at each cell pole in nearly all the cases, even though they often assemble into a cap shape (Fig. 1e,f) It is possible that higher accumulation of molecules in the cluster resulted in the ‘cap’ form the polarity of the cell could be maintained even in the overexpression condition as the number of molecules in the two clusters remained skewed in these cells we used chromosomal fusion and studied localization at native expression level high-throughput quantitative PALM molecule localization analysis pipeline The pole with more detected molecule is determined as principle pole (c,d) (i) Representative cell of single out-of-focus BF image (c) or reconstructed image from 32 z-stacks (d) Outline and segmentation are shown by a yellow line (ii) Fitting of corresponding cell in Vibio Each detected HubP molecule is plotted with ‘x’ (e,f) Histogram presentation of the localization of HubP molecules relative to the long axis of the cell determined by indicated outlining method −0.5 indicates the principle pole and +0.5 indicates the other pole it became clear that insufficient segmentation from BF image resulted in mediocre cell outlining unsuitable for proper analysis in super-resolution (see below) We decided that to reach the most precise localization the cell outlines should be drawn from images with comparable resolution Fine-scale localization analyses by Vibio (a) Representative processed PALM images of V cholerae cell expressing DronPA-MTS (green) and CrvA-PAmCherry (red) (b) 2D plot presentation of CrvA-PAmCherry molecule localization (c) Precise subcellular localization of HubP-DronPA molecules Cell outline was determined by PAmCherry-MTS Percentage of molecules localized in specific fractions (polar Total number of molecules detected and number of cells analyzed are shown in square brackets and in braces Fine-scale localization analyses of polar proteins. 2D plot and histogram presentations of DronPA fusions of FlhG (a), FlhF (b), ParC (c,d) and ParP (e,f) in hubP+ (a–c,e) or ∆hubP (d,f) background. PAmCherry-MTS was co-expressed for the determination of cell contour. Results are presented as described in Fig. 4c these proteins showed minor differences in fine-scale distribution such as fraction of non-polar single molecules and detailed positioning towards the tip of the cell pole and the outer/inner curve We hypothesize that there is a certain capacity of HubP molecules at a cell pole given accumulation of HubP molecules (presumably synthesized throughout the cell cycle) for transition from uni- to bi-polarity of HubP The cap-shaped structure is likely an ‘overflow’ of overexpressed HubP molecules here we provide means to create accurate cell outlines for super-resolution analyses of both cytoplasmic and periplasmic proteins this analysis lacked information relative to the cell pole; since we could only use 2 colours in our PALM experiments we were not able to draw precise cell outlines when two proteins were labelled with FPs our novel outlining technique and Vibio pipeline allows analysis of precise subcellular localization of molecules from dozens of super-resolution microscopy images and hundreds of cells while taking into account cell orientation correctly these methods could provide better landscapes of the subcellular organization of other cellular machineries in small bacterial cells bacterial cells are grown in LB broth or agar (1.5%) and antibiotics are used at the following concentrations when appropriate: Ampicillin 100 µg/mL Cells were grown in the M9 minimal media supplemented with glucose (0.2%) casamino acids (0.1%) and thiamine (1 µg/mL) at 37 °C with agitation (170 rpm) fluorescent protein fusions encoded on overexpression plasmid were induced with 0.02% arabinose for 1 hour except HubP-PAmCherry which was induced with 0.2% arabinose for 4 hours 1.5H) were pre-cleaned with acetone then plasma-cleaned for 10 min at 40 W with the ELMO glow discharge system (Cordouan Technology) Agarose pad (1% in 1x M9 media) was mounted on the slide glass using Gene Frame (Thermo Fisher) Thermo Fisher) were pretreated by dilution with sterile purified H2O (1:1000) followed by sonication for 5 min 1 µL of cell culture and 1 µL of beads solution were spread on the agarose pad then covered by the plasma-cleaned cover slip for microscopy Imaging was performed at the Imagif facility (Gif-sur-Yvette All images were acquired with Nikon-Stochastic Optical Reconstruction Microscopy (N-STORM) at room temperature The N-STORM was equipped with a CFI Apo TIRF SR 100 x oil immersion objective (NA 1.49) Coherent lasers emitting at 405 nm (100 mW) 561 nm (150 mW) and built-in Nikon Perfect Focus system Raw data were taken in a field of 256 × 256 pixels (40960 nm x 40960 nm) with an Andor iXon Ultra DU897 EM-CCD camera at a rate of 55 frames per second The camera and microscope were controlled with the NIS-Elements Advanced Research software (version 5.01.00) Transmission z-stack containing 32 BF images covering positions from 1.6 µm below to 1.6 µm above the focal plane were performed before and after the molecule detection Sequential acquisition option (one frame activation with 405 nm laser and one imaging with 488/561 nm lasers sequentially) used for molecule detection during 2500–5000 frames The overlapping peaks option was used to detect molecules that were in close proximity Drift correction feature in NIS-Elements was applied to image analysis and the molecule list of each colour was exported in two different text files The results were plotted with GraphPad Prism 7.0c Molecule list of the protein of interest and the cell outline information were subsequently combined by Vibio for further analysis Vibio is a Matlab based software with a graphical user interface It was confirmed to run with Matlab R2015b and R2012b versions The code is available in supplementary information (zip) the output of multifield data is not automatic but requires the manual integration of single-field data (which contains data from multiple cells) Combined data were visualized with Matlab (histograms) and Wolfram Mathematica 11 (scatter density plots) dual color PALM data were exported into two files depending on the labelling (channel) 7–10 PALM images for each construction were analysed to calculate the number of isolated and coupled molecules as well as the average distance To examine cell size and morphology of different strains cells were grown in the M9 minimal media as for PALM imaging and 1 µL of culture was spotted on the agarose pad Phase contrast images were taken with a Zeiss axio observer Z1 microscope and Evolve EM-CDD camera (Roper) and Axio vision software Image analysis was performed with MicrobeTracker The datasets generated during the current study are available from the corresponding author on reasonable request Subcellular Organization: A Critical Feature of Bacterial Cell Replication Establishing polar identity in gram-negative rods Localisation of DivIVA by targeting to negatively curved membranes Negative membrane curvature as a cue for subcellular localization of a bacterial protein a bacterial protein that anchors chromosomes to the cell poles A novel component of the division-site selection system of Bacillus subtilis and a new mode of action for the division inhibitor MinCD A function of DivIVA in Listeria monocytogenes division site selection Clostridial DivIVA and MinD interact in the absence of Min DivIVA affects secretion of virulence-related autolysins in Listeria monocytogenes Genetic Dissection of DivIVA Functions in Listeria monocytogenes Dynamic interplay of ParA with the polarity protein coordinates the growth with chromosome segregation in Streptomyces coelicolor Dynamic gradients of an intermediate filament-like cytoskeleton are recruited by a polarity landmark during apical growth Coiled-coil protein Scy is a key component of a multiprotein assembly controlling polarized growth in Streptomyces Coordination of Chromosome Segregation and Cell Division in Staphylococcus aureus Bacterial scaffold directs pole-specific centromere segregation Caulobacter PopZ forms an intrinsically disordered hub in organizing bacterial cell poles Modularity and determinants of a (bi-)polarization control system from free-living and obligate intracellular bacteria A Localized Complex of Two Protein Oligomers Controls the Orientation of Cell Polarity Two small GTPases act in concert with the bactofilin cytoskeleton to regulate dynamic bacterial cell polarity Bactofilin-mediated organization of the ParABS chromosome segregation system in Myxococcus xanthus A multidomain hub anchors the chromosome segregation and chemotactic machinery to the bacterial pole Late assembly of the Vibrio cholerae cell division machinery postpones septation to the last 10% of the cell cycle The role of FlhF and HubP as polar landmark proteins in Shewanella putrefaciens CN-32 Regulates Flagellar Number by Assisting in the Proper Polar Localization of FlhG in Vibrio alginolyticus Localization and domain characterization of the SflA regulator of flagellar formation in Vibrio alginolyticus The peptidoglycan-binding protein FimV promotes assembly of the Pseudomonas aeruginosa type IV pilus secretin The Conserved Tetratricopeptide Repeat-Containing C-Terminal Domain of Pseudomonas aeruginosa FimV Is Required for Its Cyclic AMP-Dependent and -Independent Functions A scaffold protein connects type IV pili with the Chp chemosensory system to mediate activation of virulence signaling in Pseudomonas aeruginosa Both chemotaxis and net motility greatly influence the infectivity of Vibrio cholerae Insights into Vibrio cholerae Intestinal Colonization from Monitoring Fluorescently Labeled Bacteria A Periplasmic Polymer Curves Vibrio cholerae and Promotes Pathogenesis Use of fluorescence microscopy to study intracellular signaling in bacteria Fluorescence imaging for bacterial cell biology: from localization to dynamics Applications of fluorescence microscopy to single bacterial cells Genome-scale quantitative characterization of bacterial protein localization dynamics throughout the cell cycle subpixel precision analysis of bacterial morphogenesis and intracellular spatio-temporal dynamics Oufti: an integrated software package for high-accuracy high-throughput quantitative microscopy analysis analysis and lineage tracking of bacterial cells a tool for high throughput bacterial cell detection and quantitative analysis BactImAS: a platform for processing and analysis of bacterial time-lapse microscopy movies Introduction to super-resolution microscopy Self-organization of the Escherichia coli chemotaxis network imaged with super-resolution light microscopy Single-molecule DNA repair in live bacteria Single molecule super-resolution imaging of proteins in living Salmonella enterica using self-labelling enzymes High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization SR-Tesseler: a method to segment and quantify localization-based super-resolution microscopy data Differential Management of the Replication Terminus Regions of the Two Vibrio cholerae Chromosomes during Cell Division The MinD membrane targeting sequence is a transplantable lipid-binding helix A multi-layered protein network stabilizes the Escherichia coli FtsZ-ring and modulates constriction dynamics How bacteria maintain location and number of flagella ParP prevents dissociation of CheA from chemotactic signaling arrays and tethers them to a polar anchor Coupling chemosensory array formation and localization Segregation of molecules at cell division reveals native protein localization Characterization and development of photoactivatable fluorescent proteins for single-molecule-based superresolution imaging A practical guide to evaluating colocalization in biological microscopy Mapping molecular assemblies with fluorescence microscopy and object-based spatial statistics Enzymatic assembly of DNA molecules up to several hundred kilobases Mutation in ESBL Plasmid from Escherichia coli O104:H4 Leads Autoagglutination and Enhanced Plasmid Dissemination Nanoscale Architecture of the Axon Initial Segment Reveals an Organized and Robust Scaffold ThunderSTORM: a comprehensive ImageJ plug-in for PALM and STORM data analysis and super-resolution imaging Download references We are grateful to Romain Le Bars and Laetitia Besse at the Imagif facility for their assistance on PALM for technical assistance and discussion on the project We thank Sahand Hariri for Mathematica script and Drs Nicolas Olivier for critical reading the paper This project was supported by IDEX “Biologie Intégrative des Génomes” Institute for Integrative Biology of the Cell (I2BC) Graduate School of Structure and Dynamics of Living Systems conceived and designed overall research project Molecular and genetic experiments were performed by I.A Microscopy experiments were carried out by I.A wrote Vibio script with tight communication with I.A The authors declare no competing interests Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41598-019-43051-7 The first anthological exhibition in Spain dedicated to Fernand Léger (Argentan considered a "modern classic" of the avant-garde In a career spanning more than fifty years to which he incorporated influences from other avant-garde movements towards what has been called a "new realism" a singular figuration with a special sense of monumentality industrial development and the landscape and inhabitants of the modern city The exhibition presents 101 works - 44 oil paintings and 57 drawings and gouaches - produced between 1912 and 1955 Starting with a painting with Cubist roots the exhibition traces the artist's path towards the geometrisation Urban landscapes - Discs in the City (1920) - and monumental figures with solid volumes - Woman with Flowers (1922) - became the main subjects of his work which acquired dynamism towards the end of the 1930s with more sinuous lines and the predominance of themes like dancers the iconography of the labour and leisure of the working classes seen in works like his series The Builders (1950) as the announcement of a happy future became the central theme of his art during his final years