The exhibition Who’s Afraid of Women Photographers (1839-1945) presented at the Musée d’Orsay and the Musée de l’Orangerie in 2015 was a milestone for recognition of women artists in France One of the many photographers featured was Gabrielle Hébert (1853 she was an amateur painter before marrying Ernest Hébert in 1880 an academic artist twice appointed Director of the French Academy in Rome begun at Villa Medici in 1888 and ending twenty years later in La Tronche (near Grenoble) following the death of the man she had idolised and whose place in history she largely ensured by supporting the creation of two monographic museums like many artists and writers (including Henri Rivière Maurice Denis and Émile Zola) who equipped themselves with a camera to record their and their families’ daily lives helped along by the technical and aesthetic revolution brought about by the invention of snapshot photography she organised receptions and received elite visitors She was not long in escaping her assigned duties She took a few lessons with a Roman professional and set up a darkroom to develop and print her negatives and retouch the results It was the beginning of an extremely voluminous output of photos Hardly a day passed without her taking a snapshot interspersing them with remarks that tell us how she set about taking pictures: “Je photo… Although Gabrielle Hébert shared her taste for society portraits and tableaux vivants with Luigi and Giuseppe Primoli Princess Mathilde Bonaparte’s nephews and pioneers of snapshot photography in Italy she explored all photographic genres on her own at Villa Medici still lifes and “photographic recreations”… Providing us with the viewpoint of a permanent resident who is dazzled by the palace the site and its inhabitants (artists in residence dogs and cats) as seen from the inside and in all seasons her output reveals a completely unknown aspect of life in that artistic phalanstery Her “diary in images” is the first photo-report on daily life in the institution training and creation by winners of the Grand Prix de Rome (many of whose works are now conserved by the Musée d’Orsay) as well as a laboratory for the new political relationship between France and Italy which had just been “unified” (1861) and of which Rome became the capital in 1871 It also constitutes a unique testimony on one of the first couples of creators at Villa Medici Although Gabrielle assisted Ernest with his activities as an artist retouching his paintings and even copying them it was Ernest himself who was the photographer’s real focus she watched him obsessively and was never tired of capturing him on film moments of conviviality with visitors and interactions with residents bathing in the sea and alone in his office: all these aspects of artist director and husband Ernest Hébert’s life were scrutinised and documented When she returned permanently to France with him Gabrielle stopped cultivating her passion for photography but nevertheless continued to photograph Hébert until the very end of his life determined to immortalise him through images she escaped the closed quarters formed by the Renaissance Palace and its eccentric occupants and performed her photographic swansong during a trip to Spain which she photographed with a resolutely modern eye influenced by the early days of cinema from Gabrielle Hébert’s photographic beginnings (1888) to her last images (1908) will seek to present what she made of photography and what photography made of her which she shared and exchanged with her friends and family she became recognised as an auteur and gained social status in a milieu where artistic creation was the preserve of men photography revealed her to herself: through capturing a particularly remarkable geography and era she effectively invented her own mythology she was Villa Medici’s first photographic chronicler and made a place for herself in the medium’s history Most of the works on exhibition are original prints (in 9 x 12 cm format) along with photograph albums created by Gabrielle Hébert boxes of glass plates and cameras she used Enlargements created from negatives she never printed will add further life to the presentation The itinerary will be rounded out by drawings and paintings by Ernest Hébert testimony to a story of love for a man and a country Two months after the final round of the early legislative election, unnecessarily called by Emmanuel Macron the French President final chose a new prime minister: Michel Barnier LR internal rivals such as Laurent Wauquiez and others do not view him as a potential rival Michel Barnier was born at La Tronche (Isère Department in the French Alps) He is the youngest of three sons of a man who had a small leather and textile company and a woman who was a member of the Christian left Michel Barnier graduated from the Ecole Supérieure de Commerce de Paris (ESCP) His friendship with the future prime minister Jean-Pierre Raffarin Michel Barnier has been close to Philippe Séguin and his gaullisme social until the vote on the Maastricht Treaty in 1992 separated the two That’s why some refer to Michel Barnier as a “centrist” although he is anchored more on the political right He has never belonged to a “political stable” (écurie politique) a man compatible with many political forces which is one recipe for his long political career According to the journalist Ludovic Vigogne the only real clash with Emmanuel Macron came in 2019 during the European elections when Michel Barnier supported the LR list led by François-Xavier Bellamy while President Macron thought Barnier was going to make a gesture towards the presidential majority list led by Nathalie Loiseau Michel Barnier became the youngest member of the National Assembly he successfully co-organized the Winter Olympic Games and Winter Paralympics in Albertville (French Savoie region) he was the only Gaullist not in support of Jacques Chirac but of the (economically) liberal Valéry Giscard d’Estaing he worked as chargé de mission for Robert Poujade the first French ministre de la Protection de la nature et de l’Environnement when the subject was still not high on the agenda he requested to become Minister of the Environment (1993 to 1995) during the years of the second cohabitation under the RPR Prime Minister Edouard Balladur and the Socialist President François Mitterrand Michel Barnier told the French magazine Paris Match: “You cannot become president of the Republic of you don’t like trees.” Michel Barnier is facing again a kind of cohabitation because as member of the LR group with only 47 members in the National Assembly he will govern together with President Macron from the Renaissance Party (and other forces) Michel Barnier served as Minister for European Affairs Michel Barnier was not amused when he was ousted by President Chirac just after one year as Minister of Foreign Affairs in the third government of Prime Minister Jean-Pierre Raffarin (2004 to 2005) Michel Barnier served as French Minister of Agriculture and Fisheries before embarking on a career on the European level Michel Barnier was European Commissioner for Internal Market and Services from 2010 to 2014 vice president of the European People’s Party (EPP) from 2010 to 2015 and the EU’s chief negotiator on Britain’s exit from the European Union President Macron first thought of Bernard Cazeneuve prime minister 2016-2017 under President Hollande But when Cazeneuve was inflexible regarding Macron’s pension reform pushed through thanks to the infamous (because undemocratic) Section 3 of Article 49 of the Constution (article 49 which gives the government the ability to pass bills without the approbation of the parliament Macron renounced on the nomination of Cazeneuve the extreme-right Rassemblement National (RN) of Marine Le Pen had signaled that they would immediately have censured Cazeneuve as the RN would have censured a government of the economically liberal Xavier Bertrand (LR) other serious option as prime minister President Macron was pursuing some on the left are right when they are saying that Marine Le Pen and the Rassemblement National were kingmakers when it comes to President Macrons choice of a new prime minister Marine Le Pen said: Michel Barnier is at least responding to the first criteria which we reqested that is a man who respects all policial forces a man capable to talk to the Rassemblement National the first group (in fact: party) in the National Assembly the RN National Assembly member Jean-Philippe Tanguy had qualified Michel Barnier as the most stupid politician of the Fifth Republic He immediately got a slap on the wrist by Marine Le Pen Tanguy was however right when he said that Michel Barnier was opposed to the proportionelle proportional representation in parliament would be one measure to fight voter dissatisfaction combat voters turning their back on democracy because they do not feel represented During the 2021 Les Républicains (LR) leadership election, won by Valérie Pecresse who later faltered in the 2022 presidential election and ended up with only 4.78% (!) of the vote Michel Barnier positioned himself on the right considering Eric Ciotti his main rival on that spectrum of the party He proposed a referendum on tighter immigration in France he advocated a non-European immigration freeze for three to five years he would propose to “immediately stop regularizations reduce the reception of foreign students and the systematic execution of the double penalty” He favored expanding prison capacity by 20,000 and imposing mandatory minimum sentences for certain crimes The far-right Rassemblement National went as far as accusing Michel Barnier of “plagiarism” regarding their ideas on immigration Michel Barnier ended up third in the first round of the LR primary behind Eric Ciotti with 25.6% and Valéry Pécresse with 25% (rightly) endorsed Valéry Pécresse against the far-right Eric Ciotti who infamously joined forces with Marine Le Pen Pécresse won the second round of the LR primary with 61% against Ciotti with 39% with the choice of Michel Barnier as prime minister President Macron once again anchors Renaissance to the right LR only controls 47 parliamentarians in the 577-member National Assembly Three electoral alliances ended up clearly stronger in the 2024 legislative election: the left-wing New Popular Front (Nouveau Front Populaire) with some 180 seats Macron’s electoral alliance Ensemble with 159 (they lost 89 seats!) and Marine Le Pen’s Rassemblement National and Allies with 142 seats The French instability is far from over Macron’s Renaissance and LR together are still far from a majority in the National Assembly Marine Le Pen’s Rassemblement National and the Nouveau Front Populaire could at any time sink the new Barnier government The first task of the new government will be to come forward with a reasonable 2025 budget and to balance the budget and bring down the public debt The last balanced budget dates back to 1974 Advertisements (accept cookies; we receive a commission Beauty items at Amazon.com and Amazon.co.uk Photograph by the European People’s Party Our exposures to endocrine disruptors – substances capable of interacting with our hormonal system and altering its function – are many At the Institute for Advanced Biosciences in La Tronche an Inserm team is conducting a study to determine whether changing our consumption habits could reduce our exposures The researchers have chosen to study the case of cosmetic products: soap Ksenija silently observes the cosmetic products laid out before her but they potentially contain endocrine disruptors such as phthalates Behind this jargon hide compounds that are harmful to the body They are used as plasticizers or preservatives in certain skincare and beauty products So can we reduce our exposure to these toxic substances This is what Inserm researchers at the Institute for Advanced Biosciences in La Tronche near Grenoble want to find out They are asking young female volunteers like Ksenija to stop using their cosmetic products for five days a large number of urine samples will be collected and analyzed to determine their levels of endocrine disruptors The latter have a very short lifespan and are eliminated rapidly after being absorbed by the body The whole challenge of the intervention study is to find out whether our exposure to these chemical compounds can be reduced by changing our consumption habits give recommendations to those who wish to protect their health are co-leading the Ireco study (Intervention to reduce exposure to environmental contaminants) This is the first intervention study on endocrine disruptors to be performed in France Malorie Graça (left) is recruiting volunteers to participate in the study The aim is to modify their exposure before a possible pregnancy as endocrine disruptors are suspected to interfere with the development of the fetus Ksenija has just spent five days without using her usual cosmetic products The clinical research associates Joane Quentin (left) and Valentine Socquet (middle) pay her a visit They check that the volunteer has followed the protocol correctly and that she has been able to use the mobile app the volunteer is given substitute products that do not contain the chemicals being studied as well as a telephone with an app in which she must enter the time of each use of the products as well as other potential sources of exposure such as food A blood sample is taken before and after the intervention phase Should the levels of endocrine disruptors measured in the urine decrease during the intervention phase these blood samples will be used to evaluate whether there has also been an effect on health Ksenija has collected all her urine samples from before and during the five-day period without cosmetic products The large number of samples makes it possible to monitor changes in the level of endocrine disruptors in the body over the same day The volunteers’ urine samples are stored at ‑80°C at the biological resource center of Grenoble Alpes University Hospital A total of 6,000 blood and urine samples will be stored before being analyzed The first data from the pilot study are analyzed by Nicolas Jovanovic a PhD student in epidemiology at the Institute for Advanced Biosciences Abigaël (left) has just completed her participation in the study The sociologist Maria Belen-Ojeda (right) evaluates her knowledge of endocrine disruptors and her willingness to limit her exposure to these chemicals This sociological component conducted with a multidisciplinary team is one of the novel aspects of the study one hundred volunteers will be recruited for the Ireco study The urine samples will be analyzed during the course of 2024 and will reveal whether or not reducing the use of certain cosmetic products reduces exposure to endocrine disruptors Photos : Inserm/François GuénetText : L. A Inserm stands for « Institut national de la santé et de la recherche médicale » (National Institute of Health and Medical Research) We are the only French public research organization entirely dedicated to human health through a better knowledge of living organisms and diseases innovating treatments and conducting public health research Please enable JS and disable any ad blocker Please upgrade your browser to improve your experience Non-members can add the privileges at checkout through our 30 day free trial By continuing I accept the Terms & Condition and Privacy Policy. I would like to receive Newsletter from MICHELIN Guide Save lists of your favorite restaurants & hotels you agree to the use of cookies in order to offer you content and services that are tailored to your interests Represented byUbba AgentIsabelle De La Patellière Sommaire de la ficheShareFacebookMailFavoriteFermerContents The residents hold out against an Iraqi siege 14-year-old Omid decides to stay in the city with his grandfather until his older brother returns Omid finds an abandoned boat in Abadan’s port #annecyfestival2023 #annecy2023 #annecyfestival #annecy #animation #animationcinema #iran #abadan #IraqIran #iraniraq #IranWar as well as representatives based in the U.S The organisation currently brings together more than 1,000 French cinema and TV content professionals (producers etc.) working together to promote French films and TV programmes among foreign audiences The remarkable rise of France's Louis Bielle-Biarrey - from senior debut in January 2022 to World Cup squad member in August 2023 France's Louis Bielle-Biarrey in full flight against Fiji (Photo by Sandra Ruhaut/Icon Sport via Getty Images) Louis Bielle-Biarrey should have gone to the 2023 World Rugby U20 Championship with France in July But France head coach Fabien Galthie insisted he – and fellow age-grade prodigy Emilien Gailleton – should train with the senior men’s squad for the Rugby World Cup after just three senior international appearances who has gone from senior debut to World Cup squad member in 18 months Related: France Rugby World Cup squad 2023 Download the digital edition of Rugby World straight to your tablet or subscribe to the print edition to get the magazine delivered to your door so who better to front a new look than England’s man… The Local Europe ABVästmannagatan 43113 25 StockholmSweden any one of these describes the mood and the moment perfectly well: [used with être] éméché there’s: Boire un coup de trop - which means to drink a bit too much (j'ai bu un coup de trop - "I had one too many") A romance language as poetic as French can do better Here are just a few - you just may need to be a little careful in front of the boss or the in-laws: [Être] pompette  - a lovely little phrase to describe that lovely little feeling of being just a bit tipsy when you’re at that love-the-world stage of your night out Your bar banter is the funniest it will ever be Use it like this: je suis pompette - "I'm tipsy" Déchiré - is at the other end of the night out spectrum to pompette You may have started the evening in saintly mode telling everyone Je veux pas être trop bourré(e) (I don’t want to drink too much) but you went too far and got déchiré - think 'hammered' and you'll be on the money Use it like this: je suis déchiré - "I am hammered" to break your face and is a poetically brutal French way of saying you’re 'smashed' You could also use se beurrer la gueule (buttering your face) for that delightful 'being plastered' image Use it like this: elle s'est pété la gueule - "she's smashed" Complètement pété - direct and to the point and about as poetic as a pissoir after a riot at a beer festival Don’t say it about a co-worker in front of the boss at the Christmas do Use it like this: il est completement pété - "he is completely wasted" Plein comme une barrique - (as full as a barrel) bladdered Use it like this: Il est plein comme une barrique S’arracher la face - (tear your face off) off your face S’éclater la tronche – literally to smash your face and one or several steps beyond s’arracher la face Use it like this: il s’est éclaté la tronche Se mettre la tête à l’envers  - out of your head (literally 'turn your head upside down') Use it like this: elle s'est mis la tête à l’envers Sam - Introducing the universal term for the non-drinking designated driver for the evening The expression comes from an old French government road safety campaign to promote driving without drinking The acronym stands for Sans Accidents Mortels (no fatal accidents) Use it like this: Qui se fait Sam ce soir PLS - another acronym it’s handy to know if someone in your party goes overboard PLS stands for Position Latéral de Sécurité – also known as the recovery position It can also be used to describe how you’re looking after your tender morning-after self Use it like this: Aujourd'hui je ne fais rien je suis en PLS sur mon canapé à regarder Netflix – "Today I'm not doing anything here’s a couple of other ways to describe it Gueule de bois - This literally translates as a 'wooden face' and describes the tender head churning nausea and vague sense of dread that follows a night out that perhaps went a little too far Use it like this: J'ai la gueule de bois aujourd'hui je crois que j'ai bu trop de vin à la fête – "I am hungover today I think I drank to much wine at the party." Caisse - This French slang has three meanings - but we’re not interested in the one for ‘car’ or ‘fart’ We want to know about the one that refers to someone who’s enjoyed a bit too much of the happy juice Prendre une caisse means to party hard - perhaps a bit too hard Use it like this: il avait pris une caisse pendant la soirée - "He was really caning it at the party." Please log in here to leave a comment Belgian plant engineering company Keppel Seghers has been awarded a contract for the design and construction of a new waste to energy (wte) plant in the municipality of La Tronche Under the new contract with Grenoble-Alpes Métropole intercommunal association install and commission two complete waste incineration lines each with a capacity of 82,500 tonnes per year for a consortium of seven groups of municipalities in south-western France Grenoble-Alpes Métropole leads the consortium of 259 municipalities in the South Isère region which are replacing an aging waste treatment plant The published tender results put the value of the contract at just under €168m Customer Service+49 7224 9397-701servicenoSpam@GO-AWAYeuwid.de Editorial Team+49 7224 9397-0recyclingnoSpam@GO-AWAYeuwid.com Get the latest news about developments and trends in the industry sent to you once a week free of charge by newsletter Sign up for our newsletter We use cookies and external services on our website others enhance your user experience or help us improve this website You can change your privacy settings any time by clicking privacy policy Necessary cookies are required for the correct functioning of the website Content from video and social media platforms is blocked by default. If access to these services is accepted, separate consent is no longer required when using them. You can find more information on the individual external services in our privacy policy FDN / Politics / Government / Michel Barnier Michel Barnier new Prime Minister (Flickr) In a simple communiqué from the Élysée Palace the President of the Republic appointed Michel Barnier Prime Minister He entrusted him with the task of forming a united government to serve the country and the French people Les Républicains candidate for the 2021 presidential election primary he was speaking ill of the President of the Republic Our country has not been well governed,” he said at the time Le Président de la République a nommé Monsieur Michel BARNIER Premier ministre. Il l’a chargé de constituer un gouvernement de rassemblement au service du pays et des Français. pic.twitter.com/beWhuEh42L — Élysée (@Elysee) September 5, 2024 « Notre pays n’a pas été bien gouverné. L’échec du Président sortant est clair. Emmanuel Macron a gouverné notre pays, à l’intérieur et a l’extérieur, de manière solitaire et arrogante. » Michel Barnier. (Primaire LR, novembre 2021) #PremierMinistre pic.twitter.com/vK3BxIRCU1 — Paul Larrouturou (@PaulLarrouturou) September 5, 2024 Covid-19. Michel Barnier favorable à la vaccination obligatoire « pour tout le monde » 11/07/2021 https://t.co/iC7juraYfE — VERITY France (@verity_france) September 5, 2024 Metrics details A couple was referred for exploration of repetitive abortions The man was found to be a carrier of a balanced reciprocal translocation t(9;10)(q11;p11.1) The meiotic segregation of chromosomes 9 and 10 was analysed in 5,157 spermatozoa from this translocation carrier and in 15,255 spermatozoa from three control donors using three-colour fluorescence in situ hybridisation (FISH) The theoretical viability of the different segregation patterns was performed using the computer system HC Forum developed by the Department of Cytogenetics at the Grenoble University Medical School A normal or balanced constitution was found in 56.25% of the analysed spermatozoa The tertiary 3:1 segregation mode was the most frequently observed (14.37%) adjacent-2 and 3:1 interchange modes were 12.85 The cumulative frequency of non-viable imbalance was estimated at 20.91% according to the theorical viability of the different segregation patterns Spermatozoa aneuploidy frequency was also evaluated for chromosomes X and there was no evidence of interchromosomal effect in spermatozoa from the translocation carrier FISH analysis of spermatozoa in combination with the viability theorical estimation of the different segregation patterns could be considered a useful tool for genetic counselling in carriers of reciprocal translocation The prediction of unbalanced mode depends on several factors: (1) meiotic factors (centromere and chiasma position) (2) selective factors (ascertainment of the translocation) (3) size of the translocated segments and (4) presence or absence of heterochromatin Translocations ascertained by the occurrence of repeated abortions suggest that these imbalances are unviable and the risk of abnormal progeny at birth is considered low Other translocations can be diagnosed by viable unbalanced offspring and the risk of deficiency for the progeny is high some translocations could be ascertained during exploration of a male-factor infertility associated to sperm parameter alterations potential viability of the progeny remains unknown karyotypes or fluorescence in situ hybridisation (FISH) analysis of sperm products from reciprocal translocation male carriers is a complement to epidemiological studies to evaluate the distribution of the different meiotic segregation patterns and consequently the proportion of normal This study provides FISH results on the spermatozoa segregation patterns from a male carrier of a reciprocal translocation t(9;10)(q11;p11.1) viable progeny as well as the theorical risk of spontaneous abortion are evaluated taking into account the percentage of viable and non-viable conceptus The patient studied was a 25-year-old man who was referred to our centre for infertility exploration The couple previously had four first-trimester spontaneous abortions Semen analysis revealed moderate sperm alterations with a primary asthenospermia and a teratospermia (sperm count 57×106/ml; motility (a+b) 25%; normal morphology 33%) His constitutional karyotype established with RHG banding and prometaphase chromosome preparation revealed a (9;10)(q11;p11.1) balanced reciprocal translocation Three healthy probands of proven fertility with normal semen parameters and a normal chromosome constitution were included in the study as a control group Sperm samples were fixed in methanol:glacial acetic acid for 60 min at 4°C and smeared onto clean glass slides (SuperFrost/Plus Sperm nuclei decondensation was performed for 1–2 min in 25 mM DTT Five different probes were used in the study: Three probes were required for the translocation analysis: (1) Alphasatellite centromeric probes specific for chromosomes 9 and 10 labelled with spectrum orange (CEP 9) and spectrum green (CEP 9; CEP 10) purchased from Adgenix (Voisins Le Bretonneux labelled by nick translation with biotin-dATP (Gibco BRL The efficiency of the labelling was controlled on metaphase and interphase nuclei obtained from peripheral blood cells Probes for sex chromosomes and chromosome 18 were used to evaluate an interchromosomal effect generated by the reciprocal translocation These probes were directly labelled by the manufacturer (Adgenix) with spectrum orange (CEP 18; CEP Y) or spectrum green (CEP Y; CEP X) Two FISH assays were performed: (1) a three-colour FISH using centromeric probes for chromosomes 9 (yellow colour) and 10 (green colour) and the YAC probe 9q22 (red colour); (2) a three-colour FISH with the probes for chromosomes X (green) The yellow colour was obtained by mixing an equal volume of the same probe labelled with spectrum orange and spectrum green (Adgenix) hybridisation was carried out at 37°C overnight in a moist chamber Detection of the labelling sites of the YAC probe was performed by incubations with avidin Texas-red/biotinylated anti-avidin (Cambio FISH analysis was performed with a ×100 objective in an epifluorescence microscope (DMRD Germany) equipped with a triple-band pass filter (FITC/Rhodamine/DAPI) Images were captured and produced at ×1,000 magnification with a digital imaging system (Mac Probe version 3.3; Perceptive Scientific International LTD Slides were included for scoring if the hybridisation efficiency was >95% At least 10,000 sperm nuclei were scored for the segregation analysis of the translocation and the aneuploidy assay of chromosomes X Some spermatozoa were excluded from the count: diffuse fluorescent domains excessive decondensation and overlapping nuclei Fluorescent spots were retained if they had comparable size and intensity and they were separated by at least one spot diameter RTG-banded chromosomes from the t(9;10)(q11;p11.1) carrier showing the normal and the derivative abnormal chromosomes 9 and 10 (A) Pachytene quadrivalent shows chromosome pairing between chromosome 9 and 10 Fluorescence in situ hybridisation on decondensed spermatozoa of a t(9;10) carrier using centromeric probe for chromosomes 9 (yellow) and 10 (green) and a biotinylated YAC clone (908F11) specific for chromosome 9q22 (red) corresponded to six different signal patterns: Normal or balanced spermatozoon (B) Probes were hybridised on human lymphocyte nuclei and metaphases to determine the hybridisation efficiency Triple FISH was performed on 64 metaphases and 125 interphase nuclei 98.4% of the cells displayed the expected normal diploid constitution while the remaining cells showed loss of signals due to hypoploidy or hybridisation failure A total of 5,157 spermatozoa were analysed to determine the meiotic segregation of the reciprocal translocation (Fig. 1). The numbers and percentages of each type of segregation observed are reported in Table 1 Most segregation products resulted from 2:2 segregation (78.28%) or 3:1 segregation (21.72%) The segregation alternate resulting in balanced spermatozoa (normal karyotype or balanced translocation) was the most frequently observed (56.25%) the 3:1 tertiary segregation accounted for 14.37% The adjacent-1 and adjacent-2 segregations (12.85% and 9.38% respectively) as well as the 3:1 interchange segregation (7.14%) were less frequently observed than the other segregation products 43.74% spermatozoa carried an unbalanced segregation product A total of 15,255 spermatozoa were included in the count with a rate of disomy frequency for chromosomes 9 and 10 respectively estimated to 0.28±0.05% and 0.27±0.02% The rate of diploid sperm nuclei was estimated to 0.05±0.02% Triple FISH using probes for chromosomes X, Y and 18 was performed in three colour FISH on 10,097 spermatozoa from the translocation carrier and on 30,202 sperm nuclei from the control group (Table 1) The ratio of X- to Y-bearing spermatozoa did not significantly differ from 1:1 YY and XY sperm nuclei was not significantly increased when compared to controls The same data were observed for the frequency of chromosome 18 disomy An interchromosomal effect was excluded for the chromosomes X Dual-colour FISH with centromeric probes enabled the authors to differentiate between alternate and adjacent-1 segregation because one of the breakpoints had a centromeric position the combination of these three probes did not allow to differentiate the products of an alternate segregation (normal or balanced) or between 4:0 segregations and diploid spermatozoa This data base has indicated that the most probable imbalance at birth for the (9;10) translocation was the 3:1 tertiary mode with a frequency of unbalanced live birth of 3.5% the mode adjacent-1 presents the highest frequency (12.85%) of producing viable unbalanced live birth then the mode adjacent-2 (9.38%) and finally the mode 3:1 (0.6%) The frequency of lethal unbalanced segregants could be estimated at 20.91% and corresponds to the theorical risk of miscarriages estimated in our translocation carrier The combination of FISH results and theorical risk offers more specific information for genetic counselling our translocation carrier was ascertained by recurrent miscarriages and was a candidate for an IVF procedure The estimation of imbalance at birth as well as the risk of miscarriage are data of major interest for an effective management of couples The sex chromosomes as well as chromosome 21 were the most frequently evaluated Sex chromosome disomy frequency was not significantly different from controls in most of carriers Chromosome 21 was also the most frequently affected by non-disjunctions in translocations carriers (12 of 38 probands) They observed that infertile patients with chromosomal rearrangement showed a higher incidence of diploidy and disomy compared to controls This was not detected in our patient who presented an asthenoteratospermia These results raised the question of the origin of these chromosome abnormalities: (1) Are they related to the structural rearrangement and due to an interchromosomal effect or (2) in relation to a deleterious environment responsible of sperm alterations and meiotic abnormalities These hypothesis need to be confirmed by FISH analysis of a larger number of reciprocal translocation carriers ascertained by the exploration of sperm alterations The majority of the published translocations are discovered by repetitive miscarriages and no information is reported regarding the result of semenogram Armstrong SJ and Hulten MA (1998) Meiotic segregation analysis by FISH investigations in sperm and spermatocytes of translocation heterozygotes Vidal F (1998) FISH on sperm heads allows the analysis of chromosome segregation and interchromosomal effects in carriers of structural rearrangements: results in a translocation carrier Vidal F (2000) Interchromosomal effects for chromosome 21 in carriers of structural chromosome reorganizations determined by fluorescence in situ hybridization on sperm nuclei Jalbert P (1993) Logistic regression model to estimate the risk of unbalanced offspring in reciprocal translocations Jalbert P (1993) Human reciprocal translocations: is the unbalanced mode at birth predictable Benet J (1999) Cytogenetic analysis of sperm chromosomes and sperm nuclei in a male heterozygous for a reciprocal translocation t(5;7)(q21;q32) by in situ hybridisation Jalbert P (1994) Viability thresholds for partoal trisomies and monosomies A study of 1,159 viable unbalanced reciprocal translocations Demongeot J (1996) Cartographic study: breakpoints in 1574 families carrying human reciprocal translocations Aston CE (1997) The meiotic segregation pattern of a reciprocal translocation t(10;12)(q26.1;p13.3) by fluorescence in situ hybridization sperm analysis Feingold E (1998) Meiotic products of two reciprocal translocations studied by multicolor fluorescence in situ hybridization Feingold E (2000) Is there an interchromosomal effect in reciprocal translocation carriers Cohen O (2000) Cooperation of selection and meiotic mechanisms in the production of imbalances in reciprocal translocations Boucher D (2002) Sperm analysis by FISH in a case of t(17; 22) (q11; q12) balanced translocation: case report Pearson PL (1999) Sperm analysis in a subfertile male with a Y;16 translocation Jalbert H (1980) Reciprocal translocations: a way to predict the mode of imbalanced segregation by pachytene-diagram drawing Geraedts JP (1998) Detection of structural abnormalities in spermatozoa of a translocation carrier t(3;11)(q27.3;q24.3) by triple FISH Schwinger E (1997) Molecular cytogenetic diagnostics in sperm Bresson JL (1998) Molecular analysis of the chromosomal equipment in spermatozoa of a 46 t(7;8) (q11.21;cen) carrier by using fluorescence in situ hybridization Stene J (1992) Experiences with risk estimates for carriers of chromosomal reciprocal translocations Wohlert M (1991) Chromosome abnormalities found among 34,910 newborn children: results from a 13-year incidence study in Arhus Lefort G (2001) Study of the occurrence of interchromosomal effect in spermatozoa of chromosomal rearrangement carriers by fluorescence in-situ hybridization and primed in-situ labelling techniques Sele B (1995) Meiotic segregation in males heterozygote for reciprocal translocations: analysis of sperm nuclei by two and three colour fluorescence in situ hybridization Martin RH (2001) Aneuploidy in human spermatozoa: FISH analysis in men with constitutional chromosomal abnormalities Martin RH (1994) Analysis of segregation in a human male reciprocal translocation carrier by two-colour fluorescence in situ hybridization Liebaers I (1999) Preimplantation genetic diagnosis and sperm analysis by fluorescence in- situ hybridization for the most common reciprocal translocation t(11;22) and gamete aneuploidy assessed in a t(1;10)(p22.1;q22.3) reciprocal translocation carrier by three- and four-probe multicolor FISH in sperm Van Steirteghem A (2000) Correlation between semen parameters and sperm aneuploidy rates investigated by fluorescence in-situ hybridization in infertile men Download references The experiments performed in this study comply with the current French laws Download citation DOI: https://doi.org/10.1007/s10038-003-0072-9 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article they would come down from the mountains to pose in Hébert's Roman studio at the Villa Medici they would dress in traditional costume or in the veils of the Virgin In response to the fashion for all things Italian affirming the realism of subjects ever popular at the Salon She was barely six years old when she posed in Paris for paintings by Hébert The photographer is his wife!Photography was booming when the painter Ernest Hébert arrived with his young wife to take up his second post as director of the French Academy in Rome (1885-1890) Gabrielle (1853-1934) was passionate about this new invention Equipped with one of the first portable cameras creating a unique photo-reportage on Italy the student boarders and their painter-director she discovered the artist's favourite villages and photographed the peasants – almost identical to those who had inspired him thirty years earlier exceptional both for its documentary and its aesthetic qualities has helped to enrich our understanding of Hébert and those around him It also reveals his wife's little known talent The trio's route followed the artist's favourite sites providing many subjects for later paintings: to the east of Rome – Cervara; to the south - Ceprano It was there that he found the scenes and authentic characters that were absolutely right for his paintings allowing him to record the peasant women at their daily tasks He transposed these into large compositions arranged on canvas in the studio In doing this he elevated these unassuming village women to the status of timeless figures Manon Merrien-Joly OKEn voilà un lookbook fascinant à regarder à rebours des plans monotones sur fond monochrome avec des modèles qui tirent la tronche directeur des collections femme de Louis Vuitton met en scène des personnages héroïques et courageux au sein d’une intrigue qui rassemble horreur science-fiction et héro.ïne.s badass Le designer a ainsi fait appel à des personnalités qui sont pour la plupart des habitué.e.s de la maison et incarnent le protagoniste principal d’une aventure imaginaire acteurs de la série American Horror Story actrices qui figurent régulièrement dans les campagnes Vuitton qui a composé un EP pour la marque en 2019 mais aussi Sophie Turner Tout ce petit monde est enveloppé dans une esthétique sombre et fantastique qui rappelle les magazines Pulp de vieux magazines populaires aux États-Unis dans la première moitié du 20e siècle On y devine aussi l'inspiration des Chair de Poule qu’on lisait gamins ou des romans d’horreur à la typo grinçante des années 70/80 a inspiré sa collection de son lookbook Au cours de la magique épopée qu’est Kingdom Hearts III on a l’occasion de faire la rencontre de 7 Sans-Cœur en forme de flan nommés les Sept Flantastiques Pas question ici de leur mettre sur la tronche (enfin pas trop) c’est l’heure des mini-jeux loufoques les Septs Flantastiques proposent chacun un mini-jeu pour lequel il faudra atteindre un certain score pour décrocher le gros lot Bien qu’ils ne soient pas vraiment cachés ils peuvent néanmoins être loupés facilement il faudra revenir après que le monde soit terminé un fruit est obtenu si tant est que le score obtenu soit suffisamment élevé Le niveau de la récompense se détermine par la réaction du Flantastique le but étant qu’il saute de joie pour acquérir jusqu’à 3 fruits ainsi qu’une compétence spéciale Dans le cas où vous parvenez à marquer le nombre de points requis pour que chacun des Sept Flantastiques soit aux anges, vous serez gratifié d’un Orichalque + dont vous aurez besoin pour synthétiser la Keyblade Ultima téléportez-vous sur le point de sauvegarde de la zone « Belvédère » et montez les escaliers juste devant vous (ceux de droite sur la carte) Il faut foncez dans le plus de flans possible dans le temps imparti Le flan à la fraise se trouve dans l’espace détente du premier étage de Galaxy Toys assemblez la plus haute tour possible en percutants les flans aux alentours et gardez-là aussi longtemps que vous pouvez Il faut également éviter les fraises pour ne pas perdre de flans sur votre tour prenez le point de sauvegarde de la Tour et allez dans la grotte Vous verrez le flan à l’orange à la sortie Mini-jeu : Prendre en photo les flans (7) avec le gummiphone Pas de limite de temps ici ; chaque flan effectue une action/pose particulière qu’il faudra pendre en photo au bon moment téléportez-vous sur le point de sauvegarde de l’Usine – Sous-sol et prenez la porte blanche juste à côté Dirigez-vous ensuite vers la réserve de portes ; une fois devant le « mur de portes » allez en arrière pour tomber face au flan à la banane Mini-jeu : Éliminer le plus de petits flans possible tout en évitant le gros faites usage de la magie Foudre X pour en tuer plusieurs rapidement ou appelez votre pote Simba avec le lien « Lueur du Roi » Les autres liens peuvent aussi se révéler efficaces De même les « finishmove » de certaines Keyblades s’avèrent intéressants Pour le flantastique d’Arendelle (Reine des Neiges) téléportez-vous à la Montagne du Nord – Crête montagneuse Mini-jeu : Plus ou moins similaire à celui de Monstropolis à la différence près qu’il n’y a pas de gros flan pour vous attraper (et les petits font un peu de magie) La stratégie gagnante reste la même : utilisez Foudre X ou Lueur du Roi Prenez le point de sauvegarde qui s’y trouve et allez dans la cour juste à côté Mini-jeu : Éliminez des vagues de flans en frappant les canons du fort il faudra toucher les gros flans qui se de déplacent au milieu de la horde Utilisez la cloche pour les avoir à coup sûr ou en cas de pépin Le jeu s’arrête à la fin du temps imparti où si un flan parvient jusqu’au fort Vous trouverez le dernier Flantastique dans le monde des Nouveaux Héros Téléportez-vous sur le point de sauvegarde Quartier Sud (uniquement de nuit il n’apparaît pas de jour) et grimpez tout en haut de l’un des immeubles qui se trouvent justes à côté de la Tour de Tokyo Mini-jeu : Il faut rebondir sur le plus de flan possible Rebondissez sur l’ensemble des flans de la zone avant d’aller sur ceux qui scintillent pour changer de zone au fur et à mesure vos sauts seront plus grands et la descente plus difficile à contrôler Notez que découvrir la totalité des Flantastiques vous débloquera le succès/trophée « Flangorneur » Kingdom Hearts III est disponible sur Xbox One/X et PS4/Pro Achat sur Amazon (Ps4/ Xbox One) musique dans le sang et rédacteur au cœur de sel You must be logged in to post a comment Tous droits réservés © 2025 WarLegend.net | Mentions Légales | Politique de Confidentialité En poursuivant votre navigation sur notre site, vous acceptez l'utilisation de cookies. 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