This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page Vaughan residents are welcome to enjoy four new courts at Le Parc Park on 172 Connie Cres and residents attended the grand opening for the city’s first sets of dedicated pickleball courts in Concord New addition brings the city’s pickleball court count to 30 The first set of dedicated pickleball courts at Le Parc Park are officially open in Vaughan LED court lighting and a shade structure with seating The new addition brings the city’s pickleball court count to 30 Vaughan said the city will open several dedicated pickleball courts over the next few years to help meet the continued demand for the popular sport The city plans to add new dedicated outdoor courts to Le Parc Park and the Carrville Community Centre plus an additional two shared-use courts at Ohr Menachem Park by the summer of 2025 The upcoming additions will bring the total number of pickleball courts in Vaughan to 40 “I am excited to officially welcome the residents of Ward 4 to the dedicated pickleball courts at Le Parc Park These new courts will help enhance our community and ensure everyone of all ages and abilities can have fun and be active,” Ward 4 Coun Chris Ainsworth said at Monday’s grand opening The following is a list of the 14 shared-use pickleball courts in Vaughan: Veracia Ankrah is a former reporter with YorkRegion.com Your browser is out of date and potentially vulnerable to security risks.We recommend switching to one of the following browsers: Invalid password or account does not exist Submitting this form below will send a message to your email with a link to change your password An email message containing instructions on how to reset your password has been sent to the email address listed on your account the industry has expanded rapidly – more than 20 million visitors decided to soak up some Croatian sunshine in 2024 Now one of the most visited destinations in the Mediterranean varied architecture and food influenced by various other regions around the continent continue to draw in the crowds with almost 6,000km of coastline between the mainland and hundreds of spectacular islands one thing is for certain: Croatia's beaches are where the action is at Central DalmatiaSolta is a lesser-known island than Hvar and Brač a bit more rugged and usually with fewer visitors This beach is on a bay that can be reached via boat or on a bicycle by land It’s very secluded – a place for getting away from it all and recharging to the sound of crickets and the sea splashing against the shore It's a great spot for a swimming expedition for those who do have a boat Insider's tip: There are also a few houses to rent overlooking the beach if you want to stay the night Central DalmatiaMurvica is a hidden gem far from Brač's hustle and bustle It's a real gem on balmy shoulder-season mornings when locals are going about their business and families prefer to stick to larger beaches It's got busier in recent years but has luckily retained its tranquil charm with crystal-clear waters attracting in-the-know beach bums year after year There's a small roadside convenience store on the way from the larger town of Bol offering cold drinks and ice creams in the summer months but be wary of how reliable opening hours can be and come prepared if you're planning on lazing around all day Insider's tip: Just a short walk up the hill from the sand is a tavern serving local dishes Central DalmatiaThis pebbly stretch of beach sits slap-bang at the heart of the island's south and attracts plenty of sun worshippers due to its close proximity to Bol Commit to the spot for an afternoon and rent sun loungers and umbrellas (expect to pay at least €20 per person in peak season) roam into the evening via one of the cocktail bars along the shore or over aperitivo in a drinking den on the edge of Bol Insider's tip: There are also great views across the clear water to Zlatni Rat beach and because of the soft sand and sea depth the beach is the locals’ favourite spot for picigin – a particularly regional ball game played in ankle-deep water and adapted from water polo in the early 20th century Local kids can often be seen jumping from the small cliffs further out Insider's tip: Another locals’ favourite is Caffe Bar Firule there's an inflatable green-and-yellow aqua park a lovely waterside promenade shaded by pine trees leads to the village of Bol Beyond is the Vidova Gora mountain – the highest peak on the Adriatic islands Insider's tip: Try windsurfing with Big Blue Sport Central DalmatiaThe water's as clear as can be a handful of spots stay shaded until the early evening All these selling points make Punta Rata a popular choice among families who flock here on peak summer days after parking their car nearby (expect a daily fee) Sunbed hire comes cheaper than rival beaches along these shores and it's a small price to pay for an afternoon on one of the best beaches in Croatia so there's little worry of the rental car being caked in sand Insider's tip: Spend a day hiking the footpaths that crisscross the rugged slopes in Biokovo Nature Park its vineyards and olive groves surrounded by rugged slopes that are dotted with old stone farmhouses and cypresses King Edward VIII whisked Wallis Simpson to Rab for a romantic break – they got special permission from local authorities to skinny-dip at Kandarola beach an event now seen as the birth of naturism in Croatia Kandarola remains nudist-friendly to this day close to the town of Lopar on Rab's north coast nearby Paradise Beach is an almost mile-long arc of sand giving onto a shallow bay complete with water sports and volleyball Insider's tip: Join a guided sea-kayaking tour around the coast with Rab-based Sea Kayak Croatia the hilltop city of Lubenice is a huddle of medieval stone cottages and chapels a steep winding footpath leads down to a secluded bay – it’s a 40-minute walk The reward is a blissful white-pebble beach that looks out across the sparkling Adriatic ask your skipper to also make stops at Cres town and the village of Valun If you're travelling with small children translucent water and an inflatable aqua park while the neighbouring Val Sundance Sandy Family Beach appeals to teenagers with its banana boats Insider's tip: Explore the depths of the Adriatic with Girandella Diving which offers scuba courses for adults and children of all levels the place is illuminated by flickering candles and flaming torches as the restaurant serves Mediterranean dishes on the terrace Mulini Beach is a pebble cove with a polished concrete bathing deck parasols and a bar serving cocktails and colourful salads Stay until early evening for DJ music and Mojitos with crushed ice and fragrant mint Insider's tip: Inland Istria is famed for its tartufi, or truffles – drive to restaurant Toklarija to sample the best of the bunch trek or cycle along marked trails in Cape Kamenjak Nature Park on the Premantura peninsula Insider's tip: Premantura is also a great spot for windsurfing – try Windsurf Station on the nearby Skoljić beach lined with sunbeds overlooking the Kvarner Gulf towards Rijeka and Krk and Cres islands is slumberous and villagey for most of the year Only in high summer does it fill up with visitors who arrive by excursion boats from Opatija The beach backs on to a row of old stone cottages that host seasonal cafés and ice-cream parlours Behind Sipar rise the pine-wooded slopes of the Učka mountain – a vertiginous path leads up to the medieval village of Mošćenice be sure to walk the five-mile seaside promenade from Lovran village to the city of Volosko in Opatija colours change from turquoise to emerald to inky blue at this wide south-facing bay with a shimmering white-pebble beach and pinewoods with a sandy seabed and shallow water – wade in far from the shore and the sea will remain only knee-deep straw parasols and a couple of makeshift beach bars and is served by boat from the city of Zadar on the mainland Insider's tip: On Zadar's seafront promenade don't miss two quirky contemporary installations following a path through fragrant Mediterranean vegetation Insider's tip: Lopud is served by Jadrolinija ferry from Dubrovnik's Gruž port which also calls at the neighbouring islands of Sipan and Koločep a secluded bay rimmed with a curving stone-and-concrete quay increasing slightly for more privacy on a wooden deck or for a group package including bottles of Champagne Organised bathing began on the island in 1908 – today this beach is lined with sunbeds and umbrellas and offers jet skiing where you'll find pebble beach Oprna Bay – park and hike the last stretch on a steep Insider's tip: The Baška Glagolitic Path is a hiking route marked by 34 stone sculptures celebrating Glagolitic script (the oldest Slavic alphabet Insider's tip: There’s another cave nearby, 65 feet above sea level, called Badanj, where remains from the Neolithic period have been found. The honorees will be recognized at the annual Women of Influence Conference in Lake Tahoe, CA on July 23. Since 1983 has recognized a growing number of female CRE professionals for their remarkable achievements has collected nominations across various categories spanning the entire commercial real estate spectrum in effort to shine a light on the individuals that have personally impacted the market and significantly driven the industry to new heights via their outstanding successes GlobeSt. is proud to announce this year's Women of Influence winners The 2024 honorees will be profiled in a feature article on GlobeSt.com in July and will also be honored at an awards dinner during the annual Women of Influence Conference in Lake Tahoe, CA on July 23rd. To register for the 2024 Women of Influence conference, or to gain more information on the event, please click here.   Congratulations to this year's honorees. The 2024 Women of Influence winners are as follows: SHARE ON FACEBOOK SHARE ON TWITTER Ingrid Tunberg Ingrid Tunberg sits on the editorial team as a coordinator and reporter for Real Estate Forum and GlobeSt.com assisting with industry awards and marketing nomination events Ingrid worked as a copywriter across various industries throughout New York City and Chicago More from this author ⟶ teams and firms that continually drive the sector’s performance as the 2025 Influencers in Industrial Nominate a candidate before the May 14th deadline Multifamily leasing and NOI may see some challenges in the current market Announcing the 2025 Women of Influence winners who will be recognized at the annual Women of Influence conference in Denver The Great Slowdown: Domestic Migration Into 2025 Dive into the data to explore domestic migration patterns over the past four years -- and uncover states and metro areas emerging as relocation hotspots in 2025 Browse More Resources › Leveling Up: Choosing the Best Renters Insurance Partner for Your Multifamily Housing Portfolio Picking the right renters insurance can be a headache This guide will help you find the perfect partner for your multifamily properties so you can boost resident participation and lower your risk The Return to Office: Recovery Still Underway Are you noticing unexpected shifts in office occupancy and commuter behavior This report reveals how evolving work patterns are challenging CRE brokers and offers crucial Discover a detailed analysis of office visit fluctuations real-world examples to inform strategic decisions and actionable metrics to guide client advising / For questions about GlobeSt.com, please call 800-458-1734 (9:00am-5:30pm ET, Monday through Friday, except holidays), or send an email to [email protected] Get Recruited Faster with a Player Profile on SoccerWire.com – The New England Revolution Academy has unveiled its roster for 2019-20 U.S The 2019-20 season also marks the formal launch of the Revolution Academy’s Residency Program The Revolution will have players living full-time in a local residence that was recently acquired by the club Players will be living full-time under the supervision of Academy staff This new program will see players from New England and other parts of the country benefit from the club’s fully-funded Academy program by providing them with a living space in close proximity to Gillette Stadium The Revolution Academy is coming off the 2018-19 season which saw seven different players receive international call ups to U.S Soccer Youth National Team camps and squads as well as multiple first team training opportunities Two Academy alumni signed Homegrown contracts with the first team beginning with Nicolas Firmino in December 2018 followed by forward Justin Rennicks in January 2019 it marked the third consecutive year the U-19s have qualified for a USSDA playoff spot and the team’s second Northeast Division title in three years while welcoming in 13 new players; all four age groups consist of a combination of both new and returning players “We’re thrilled to begin yet another chapter in our Academy’s history,” Revolution Director of Youth Development and Under-15 Head Coach Bryan Scales said “We’re very excited for the upcoming season and to track the progress and success of our players across each age group.” the Revolution Academy is the only fully-funded professional youth development program in New England Soccer Development Academy as well as the MLS Generation adidas Cup Qualified and talented players are accepted into the program without regard to their financial situation All players’ needs and expenses related to soccer activities – training travel associated with competitions – are provided by the Revolution at no cost to the players or their families The complete Revolution Academy roster, including coaching staffs for each age group, is below. For more information about the New England Revolution’s youth teams and Revolution Academy programming, please visit revolutionsoccer.net/academy United Academy have announced that Dave Sanford and Gus Teren have been promoted to Academy Director and Technical Director Under-17 Men’s National Team head coach Raphael Wicky has named a 23-player roster for the 4 Nations Tournament in Assen The New York Red Bulls have added new coaches and training staff ahead of the Fall USSDA academy Inter Miami CF announces its technical and coaching staff for the Academy ahead of the 2019-20 season Join the SoccerWire College Soccer Recruiting Search Engine and learn how to be seen OVER 1 MILLION TIMES PER YEAR This site uses cookies to give you the best possible experience but do not collect personal information By continuing to use the site you agree that we can save cookies on your device Metrics details are fundamental regulators of gene expression and the MYC locus utilizes a diverse regulatory architecture driven by multiple CREs To better understand differences in CRE function we perform pooled CRISPR inhibition (CRISPRi) screens to comprehensively probe the 2.8 Mb topologically-associated domain containing MYC in 6 human cancer cell lines with nucleotide resolution We map 32 CREs where inhibition leads to changes in cell growth including 8 that overlap previously identified enhancers Targeting specific CREs decreases MYC expression by as much as 60% we find that these CREs almost always contact MYC but less than 10% of total MYC contacts impact growth when silenced highlighting the utility of our approach to identify phenotypically-relevant CREs We also detect an enrichment of lineage-specific transcription factors (TFs) at MYC CREs and tumor-specific correlation between TF and MYC expression not found in normal tissue these CREs represent systematically identified functional regulatory regions and demonstrate how the same region of the human genome can give rise to complex prior studies have focused typically on one or a few CREs in a single tissue/cell making comparisons across tissues/cells challenging many of these studies rely on large deletions that make it challenging to delineate precise CRE boundaries By deploying a uniform set of ~112,000 perturbations across the 6 cell lines we identify previously unknown CREs that regulate MYC and define their boundaries at nucleotide resolution Loss of CCAT1 transcript reduces also reduces looping of the CCAT1 locus with the MYC promoter demonstrating that a noncoding RNA can itself modulate the regulatory impact of a noncoding DNA CRE restoration of CCAT1 expression in trans can partially rescue the growth reduction of CCAT1 repression at the native CRE We find that identified CREs harbor motifs for lineage-specific transcription factors and are in direct physical contact with the MYC promoter via dynamic this comprehensive dissection of functional MYC CREs demonstrates the utility of perturbing noncoding CREs across different cell/tissue types to elucidate the complex context-sensitive regulatory architecture of an important human oncogene a Data from the Encyclopedia of DNA Elements (ENCODE) and the Catalog Of Somatic Mutations In Cancer (COSMIC) for quantifying diversity of regulatory landscapes b Schematic of regulatory diverse over different cell lines for each biochemical signatures of enhancer activity H34me1 and DNAse I hypersensitivity (regulatory features of enhancer activity) were compared among 386 oncogenes (from COSMIC) across different cell types to assess regulatory diversity We compute an oncogene regulatory diversity (ORD) score for each oncogene; a high score indicates greater diversity across cell types c Snapshot of ENCODE data for all 386 oncogenes across 10 cell lines and 3 biochemical features visualizing a 2 Mb window around each oncogene d Ranking of COSMIC oncogenes by ORD across biochemical signatures The oncogene MYC has the greatest regulatory diversity across cell lines P values are calculated by the robust rank aggregation algorithm e H3K27ac near a high diversity oncogene (MYC) and a low diversity oncogene (ARAF) across 10 cell lines Source data are provided as a Source Data file Given the high regulatory diversity of MYC with many biochemical hallmarks of cell-type-specific enhancers we decided to systematically interrogate CREs across cell types at the MYC locus a CRISPR inhibition (CRISPRi) screen tiling across a 2.8 Mb topologically-associated domain containing the oncogene MYC using ~112,000 gRNAs b MYC expression and CRISPR screen depletion (Chronos) scores from Broad DepMap (n = 1021 cell lines) c Sliding window analysis for identification of cis-regulatory elements (CREs) d Upset plot of shared and cell-specific enhancer-like CREs across six pooled CRISPRi screens we screened more than 700,000 genetic perturbations across 6 human cell lines the MYC locus tiling gRNAs and the non-targeting negative controls had a similar distribution with far less depletion than essential gene-targeting gRNAs those in red text match a previously MYC enhancer found in the same tissue/cancer type The other 24 CREs have not previously been described or have not been described in the same tissue/cancer This highlights the tremendous potential for discoveries using uniform tiling of perturbations in different cell lines from different tissues—even for a relatively well-studied genetic locus like MYC it is not known whether all bases in these regions are required for CRE activity we find the CREs from the CRISPRi screens are smaller and provide better localization of key functional elements one in a lung cancer and another in breast cancer whereas in other cells we find either one enhancer or the other is present but not both (e.g This highlights the ability of the CRISPR tiling approach to further elucidate functional subregions within even known enhancers dissecting differential usage of distinct CREs contained within a larger enhancer cluster like MYC-LASE This difference is possibly due to the Fulco et al screen targeting specific regions within the MYC TAD instead of tiling the entire TAD a Locations of indicated CREs within the MYC TAD b Cell proliferation (CellTiter Glo) for CRISPRi perturbations of CREs identified in the TAD-wide screen (n = 3 biological replicates of 2–3 gRNAs per CRE or non-targeting control) c MYC expression after CRISPRi perturbations of CREs identified in the TAD-wide screen (n = 3 biological replicates of 2–3 gRNAs per CRE or non-targeting control) Boxplot whiskers extend from minimum to maximum the box extends from 25th to 75th percentile and the mid-line represents the median d Heatmap of H3K27ac and chromatin accessibility from specific cell lines (x-axis) for CREs found in the indicated cell lines (y-axis) H3K27ac and chromatin accessibility biochemical signatures are rarely found in cell lines where the CRE is not functional e Overlap of CREs with enhancer and promoter-associated biochemical signatures (n = 5 association tests with 2000 samples per test) Error bars represent mean ± SD of association tests We find strong concordance between decreased MYC expression and decreased growth suggesting that CREs identified in the TAD tiling screen work through modulation of MYC expression a Schematic of H3K27ac HiChIP workflow b (left) H3K27ac HiChIP contacts at 20 kb resolution between MYC and other elements in the TAD Highlighted in color are the significant CREs identified in each CRISPRi screen and their HiChIP contacts with MYC (right) The percent of CRISPRi-identified CREs that contact MYC (first column) and the percent of total contacts with MYC that include CRISPRi CREs (second column) c Pearson correlation of H3K27ac HiChIP from A549 cells with histone modifications d Recall analysis of gRNA sequences within CREs by the indicated biochemical signature Plot represents an average across all cell lines e Overlap of H3K27ac HiChIP and CRISPRi perturbation screens for A549 (lung) These three cell lines share a CRE (dotted box) at the promoter of CCAT1 This analysis reveals that a subset of CREs detected in the CRISPRi screens have strong eRNA transcription; however eRNA transcription is not directly linked to functional impact on cell growth To dissect the role of the noncoding RNA transcript from the activity of the DNA elements of the CRE we used three distinct approaches: targeting the CRE using DNA-targeting (Cas9) and RNA-targeting (Cas13) CRISPRs performing physical genome conformation mapping (H3K27ac HiChIP) after CCAT1 RNA knockdown and examining changes in CRE looping and measuring whether the impact of CCAT1 silencing could be rescued by overexpression of CCAT1 in trans suggesting that the CCAT1 lncRNA might promote changes in genome structure for other nearby colon cancer-associated loci We found the cells with CCAT1 silenced and expressing a (non-targetable) CCAT1 transgene were rescued but not completely (84% of control cell growth) CCAT1 RNA delivered in trans can restore at least partial function These results suggest that the CCAT1 RNA plays an essential role in driving the activity of the CRE the overlap between many CRISPRi-identified CREs at the MYC locus and ncRNA promoters suggests that this might be a more general principle of gene regulation a Identification of transcription factor binding at CREs and summary of key transcription factors found b Number of transcription factors significantly enriched in the CREs from 1 or more cell lines c Fold enrichment for top 25 transcription factors in each cell line d Transcription factor ChIP-seq for selected transcription factors from panel c THAP11 ChIP-seq is from HepG2 and ETS1 ChIP-seq is from SCC25 e Correlation in primary tumors from The Cancer Genome Atlas (TCGA) between MYC expression and top-ranked transcription factors from panel (c) TCGA tumor types are matched to the same cell/tissue type as CRISPRi cell lines in which the transcription factor is enriched (n = 2855 TCGA primary tumors; LUAD: n = 512 Recent advances in genome editing technology have enabled researchers to manipulate genomic elements in order to understand which ones impact key phenotypes and how precisely they work most studies of CREs have been limited to a single cell type and it is unclear whether these elements are unique to the cell type studied or are shared among many distinct cell types we have used pooled CRISPR inhibition (CRISPRi) screens to identify and characterize the CREs of the oncogene MYC across six cancer cell lines in a systematic These CREs modulate cell growth and do so via changes in MYC This is likely via enhancer activity: Nearly all are in physical contact with the MYC promoter and contain binding sites for characteristic lineage-associated transcription factors for each respective tissue/cancer it can be challenging to compare between those that use different kinds of perturbations (e.g A hallmark of our study is the uniform set of ~112,000 perturbations across 6 cell lines for the study of human cancer and gene regulation the use of human models is preferable where possible Our study uses a single method (tiling CRISPR silencing) to investigate CREs systematically and thoroughly in the MYC TAD across many different cell lines yielding a more comprehensive enhancer discovery at the MYC locus This unified approach can help develop unique insights: For example we discover that a previously known colon cancer enhancer is not only present in the colon cancer cell line The scale of these noncoding screens at the MYC locus is vast: We perturb every base pair in the MYC TAD across 6 human cell lines to systematically discover known and new enhancers We find that CRISPRi CREs are strongly associated with certain biochemical features such as enhancer-associated histone marks and chromatin accessibility neither these marks nor 3D proximity with a gene target (as measured by H3K27ac HiChIP) are sufficient to conclude that a CRE is functional we performed an in-depth validation showing that they alter cell growth and MYC expression genetic perturbations have no effect on either cell growth or gene expression One important limitation of this work is that each CRE was examined individually and it is difficult to disentangle whether these CREs may act redundantly or synergistically Future work with simultaneous perturbation of multiple CREs or a combination of activation and inhibition at different CREs could help address this question a similar atlas for CREs would be an enabling resource for enhancer genomics single and combinatorial CRE targeting might yield effective strategies to selectively target this important oncogene in a cell-type specific manner We acquired cell lines from ATCC (except HEK293FT, which were from ThermoFisher) and maintained with the media listed (Supplementary Data 6) along with puromycin concentrations for selection Plates were imaged using an Incucyte SX3 at 10× magnification and acquiring 9 images per well every 4 h for 72 h We electroporated the concentrated ligation product with Endura cells (LGCBiosearch 60242-2) in multiple transformations and then pooled together and plated on square 245 × 245 mm BioAssay plates (Corning 431111) with LB / agar 100 ug/ml Carbenicillin we measured a representation of 500 colonies per gRNA The bacteria were harvested after 14 h of incubation at 37 °C scraped with 5-10 ml of LB media and processed with a PlasmidPlus maxi prep kit (Qiagen 12965) We used 1 maxi prep column per 0.25 g of bacteria HEK293FT cells were plated in Dulbecco’s Modified Eagle Medium (DMEM) + 10% FBS (D10) in a T225 flask so that the following day Half of the media was removed from the flask and cells in each flask were transfected with 25 ug of transfer vector (e.g and 20 ug psPAX2 (packaging plasmid) using 2.5 mL Opti-MEM and 136 ul polyethylenimine linear 25 K (Polysciences 23966) we collected the viral supernatant and put it immediately on ice We concentrated the supernatant by centrifugation at 100,000 × g (Thermo Sorvall LYNX) for 2 h at 4 °C The resulting pellet was resuspended in cold DMEM and stored at −80 °C until use After all the cells in the no virus well had died cells in the corresponding wells (with puromycin) were counted to determine the viral volume that results in 20%–40% cell survival corresponding to a multiplicity of infection (MOI) of 0.2–0.5 We isolated genomic DNA from cells using a modified salting-out precipitation117 The gRNA readout was performed using two rounds of PCR we used 10 ug of gDNA in each 100 ul reaction We pooled the PCR1 products and used the mixture for a second PCR reaction This second PCR adds on Illumina sequencing adaptors and barcodes We ran 1 PCR2 reaction for every 10,000 gRNA in the library for a total of 12 PCR2 reactions per biological conditions We performed PCR1 reactions using TaqB polymerase (Enzymatics P7250L) and PCR2 reactions with Q5 (NEB M0491) We pooled and purified PCR2 reactions with a QIAquick PCR purification kit We ran the resulting product through a 2% E-gel from which we extracted a band of 250–270 bp and purified it with a QiaQuick Gel Extraction kit (Qiagen 28704) We quantified the concentration of the gel-extracted PCR products using Qubit dsDNA HS Assay Kit (Thermo Fisher Q32851) then diluted and sequenced it on an Illumina MiSeq using a v3 kit (Illumina MS-102-3001) This was followed by sequencing on an Illumina NextSeq using a NextSeq 500 High Output v2 kit (150 cycles We identified significant CRISPRi screen peaks (CREs) by merging significant gRNAs that were within 500 nt of each other was defined as one with gRNAs no more than 500 nt apart from each other We titered viral volumes such that 20-40% of cells survived after puromycin selection cells were cultured for 3 days and collected for qPCR After selection and expansion for ~2 weeks in puromycin we first normalized the target gene expression by B2M expression per sample we normalized each sample by the average value of the non-targeting (negative control) gRNAs we used Taqman Universal PCR Master Mix (Thermo 4304437) HPRT1 endogenous control (VIC/MGB probe) (Thermo 4326321E) and for gene probes we used Taqman Gene Expression assay (Thermo 4453320 and 4331182) we plated ~2000 cells in 100 μl of media in black-walled 96-well plates (Corning 3904) We waited for the fastest growing cells to reach ~80% confluency then lysed all cells using a 1:4 dilution of Cell Titer Glo and PBS We placed the plates on an orbital shaker for 2 min we quantified the luminescence signal with a Tecan Infinite 200 Pro Plate Reader The resulting bam files were sorted and removed of duplicates We then generated bigwig coverage files using deeptools 3.4.2 with normalization for sequencing depth using the RPGC method We then used them as input for genomation for scoring and heatmap generation Heatmap windows were defined as significant enhancer peaks found across each cell line These peaks were the same as those defined in CRISPRi pooled screens library preparation and data analysis The workspace for GAT was defined as the entirety of the MYC TAD / CRISPRi screen region GAT was run 5x with 2000 iterations per run for a total of 10,000 iterations per association test We fixed cells in 1% formaldehyde for 10 min and quenched to a final concentration of 125 nM glycine We mixed 2 million fixed mouse cells with 10 million of each fixed human cancer cell line resuspended in nuclear lysis buffer and sonicated using a Covaris LE220 with the following conditions: Fill level 10 We incubated the sheared DNA with Dynabeads Protein A (ThermoFisher 10001D) for 2 h at 4 °C We then placed the tubes on a magnet and the supernatant was kept We performed immunoprecipitation with a cross-species reactive H3K27ac antibody (Active Motif 39133) The samples were incubated with the antibody overnight at 4 °C eluted and treated the samples and treated with Proteinase K We purified the samples using Zymo DNA Clean & Concentrator Biotin capture was performed with Dynabeads M-280 Streptavidin (ThermoFisher 11205D) We purified the amplified libraries with Illumina Sample Purification Beads We sequenced the libraries using paired-end reads with either Illumina NovaSeq 6000 S2 Reagent Kit v1.5 (200 cycles 20028315) or NextSeq 500 High Output v2 kit (150 cycles 20024907) to generate 100–200 million read pairs per sample We used H3K27ac ChIP-seq data as a reference set of peaks in the FitHiChIP pipeline All constructs were confirmed by Sanger sequencing We lentivirally transduced guide constructs into the TetO-RfxCas13d-NLS HT29 cells with 3 biological infection replicates. After 24 h, we replaced the media with puromycin media (Supplementary Data 6) after completed puromycin selection (72 h) we added 1 μg/ml doxycycline (Sigma D9891) to induce RfxCas13d expression We titered viral volumes such that 20–40% of cells survived after puromycin selection After selection and expansion for 3 days in puromycin we plated cells for DNA/RNA extraction for qPCR Default settings were using to remove duplicate reads We then identified statistically significant loops in each condition (nontargeting or CCAT1 knockdown) using the same threshold as in the prior HiChIP (1% FDR) keeping all loops that were significant in either condition Loop strengths were normalized so that nontargeting and CCAT1 knockdown conditions had the same mean across all loops Bootstrap error in contact count was calculated by re-sampling 90% of loops (without replacement) and computing the standard deviation from 1000 bootstrap resamples Significance was calculated from the z-score of the distribution of loop strength differences (CCAT1 knockdown—nontargeting control) assuming a normal distribution the log-fold change was computed comparing the CCAT1 knockdown loop strength to the nontargeting control loop strength with a pseudocount added to both quantities before the fold-change calculation We chose a pseudocount equivalent to the minimum number of contacts (8) We termed this CCAT1 rescue plasmid pLentiCCAT1-Red (Addgene 226521) we seeded 9 million HEK293FT cells into a T75 flask and transfected them with 45.6 µl polyethylenimine linear MW 25000 (Polysciences) We then transduced HT29-KRAB-dCas9 cells with pLentiCCAT1-Red (MOI < 0.1) we sorted transduced cells (mKate2-positive cells) using a Sony SH800 cell sorter We termed these cells HT29-CRISPRi-CCAT1rescue Lentivirus was produced as in MYC knockdown using Cas13 HT29-CRISPRi and HT29-CRISPRi-CCAT1rescue were each transduced with the guide RNA constructs in lentiGuideFE-mSG-Puro (MOI ~ 0.5) all cells where plated onto a 96-well plate at 4000 cells per well Plates were imaged using an Incucyte SX3 at 10× magnification with 4 images acquired per well every 4 h the relative growth rate was calculated as the mStayGold (green) area divided by the total cell confluence area and then normalized to the cells transduced with nontargeting gRNAs We downloaded NET-CAGE data from Hirabayashi, et al.86 We compared NET-CAGE signal within the identified CREs by summing the total NET-CAGE reads We ranked 20 kb (HiChIP) bins and DHS peaks within the screen region by the sum of NET-CAGE reads We downloaded predicted transcription factor motif binding sites from JASPAR (Supplementary Data 1) After choosing sites with a score (weight) greater than 400 we separated each transcription factor’s binding sites into a separate bed file we used genomic association tester (GAT) 1.3.4 to associate significant gRNAs in each cell line (as defined in CRISPRi pooled screens library preparation and data analysis) with the transcription factor motifs in each transcription factor’s file We defined the workspace for GAT as the entirety of the MYC TAD / screen area GO term enrichment was performed with Metascape v3.5 against a background of all Homo sapiens transcription factors in JASPAR We downloaded TF ChIP-seq data from ENCODE (Supplementary Data 1) as processed bigWigs We accessed TCGA pan-cancer RNA-seq data via the cBioPortal dashboard We generated MYC-TF correlation plots in RStudio which also gave us correlation values and p values TFs chosen are among the top 50 TFs ranked by fold enrichment from GAT (See Transcription factor motif prediction) and represent TF families highlighted by GAT in each cell line Data between two groups were compared using a two-tailed unpaired Student’s t test a P-value less than or equal to 0.05 was considered statistically significant for all analyses and not corrected for multiple comparisons In cases where multiple comparison corrections were necessary we adjusted the P value using the Benjamini-Hochberg method All group results are represented as mean ± s.e.m Statistical analyses were performed in Prism (GraphPad) and RStudio (RStudio PBC) Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article H3K27ac HiChIP data generated in this study is available have been deposited in NCBI’s Gene Expression Omnibus repository under GEO Series accession number GSE278245. Source data are provided with this paper An argument for the genetic simplicity of man and other mammals An integrated encyclopedia of DNA elements in the human genome Transcriptional regulatory elements in the human genome Integrative analysis of 111 reference human epigenomes 8.2% of the human genome is constrained: variation in rates of turnover across functional element classes in the human lineage An upper limit on the functional fraction of the human genome PAZAR: a framework for collection and dissemination of cis-regulatory sequence annotation Expanded encyclopaedias of DNA elements in the human and mouse genomes An atlas of dynamic chromatin landscapes in mouse fetal development The Cancer Genome Atlas Pan-Cancer analysis project Integrative pan cancer analysis reveals epigenomic variation in cancer type and cell specific chromatin domains Combinatorial patterns of histone acetylations and methylations in the human genome Histone modifications at human enhancers reflect global cell-type-specific gene expression Distinct and predictive chromatin signatures of transcriptional promoters and enhancers in the human genome Systematic analysis of binding of transcription factors to noncoding variants Histone H3K27 acetylation is dispensable for enhancer activity in mouse embryonic stem cells Histone H3K4 monomethylation catalyzed by Trr and mammalian COMPASS-like proteins at enhancers is dispensable for development and viability Enhancer redundancy in development and disease Shadow enhancers are 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β-Thalassemia High-resolution CRISPR screens reveal fitness genes and genotype-specific cancer liabilities Identification and characterization of essential genes in the human genome A Myc enhancer cluster regulates normal and leukaemic haematopoietic stem cell hierarchies A NOTCH1-driven MYC enhancer promotes T cell development transformation and acute lymphoblastic leukemia Mice deficient of Myc super-enhancer region reveal differential control mechanism between normal and pathological growth Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Long-range enhancers regulating Myc expression are required for normal facial morphogenesis Mice lacking a Myc enhancer that includes human SNP rs6983267 are resistant to intestinal tumors Role of SWI/SNF in acute leukemia maintenance and enhancer-mediated Myc regulation Active medulloblastoma enhancers reveal subgroup-specific cellular origins The MYC enhancer-ome: Long-range transcriptional regulation of MYC in cancer Massively parallel Cas13 screens reveal principles for guide RNA design Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens COSMIC: The catalogue of somatic mutations in cancer The GTEx Consortium atlas of genetic regulatory effects across human tissues A long-range Shh enhancer regulates expression in the developing limb and fin and is associated with preaxial polydactyly Genome-wide mapping of Myc binding and gene regulation in serum-stimulated fibroblasts Endogenous Myc controls mammalian epidermal cell size endoreplication and stem cell amplification The transcription factor Myc controls metabolic reprogramming upon T lymphocyte activation MYC activation is a hallmark of cancer initiation and maintenance Diverse karyotypic abnormalities of the c-myc locus associated with c-myc dysregulation and tumor progression in multiple myeloma Identification of c-MYC as a target of the APC pathway NOTCH1 directly regulates c-MYC and activates a feed-forward-loop transcriptional network promoting leukemic cell growth Notch1 contributes to mouse T-cell leukemia by directly inducing the expression of c-myc c-Myc is an important direct target of Notch1 in T-cell acute lymphoblastic leukemia/lymphoma The landscape of somatic copy-number alteration across human cancers The Myc oncoprotein as a therapeutic target for human cancer Pan-cancer alterations of the MYC oncogene and its proximal network across the cancer genome atlas Therapeutic targeting of ‘undruggable’ MYC Crosstalk between MYC and lncRNAs in hematological malignancies lncRNAs and MYC: An intricate relationship Genomic copy number dictates a gene-independent cell response to CRISPR/Cas9 targeting CRISPR screens provide a comprehensive assessment of cancer vulnerabilities but generate false-positive hits for highly amplified genomic regions Improved vectors and genome-wide libraries for CRISPR screening c-Myc overexpression sensitises colon cancer cells to camptothecin-induced apoptosis Targeting c-Myc to overcome acquired resistance of EGFR mutant NSCLC cells to the third-generation EGFR tyrosine kinase inhibitor BRD4 modulates vulnerability of triple-negative breast cancer to targeting of integrin-dependent signaling pathways Evaluation and design of genome-wide CRISPR/SpCas9 knockout screens Genome-scale CRISPR-mediated control of gene repression and activation Genome-wide association study of prostate cancer identifies a second risk locus at 8q24 Super-enhancers in the control of cell identity and disease AB cell regulome links notch to downstream oncogenic pathways in small B cell lymphomas GAT: A simulation framework for testing the association of genomic intervals Measurement of differential chromatin interactions with absolute quantification of architecture (AQuA-HiChIP) Activity-by-contact model of enhancer–promoter regulation from thousands of CRISPR perturbations The role of enhancers as centres for general transcription factor recruitment Widespread transcription at neuronal activity-regulated enhancers A large fraction of extragenic RNA pol II transcription sites overlap enhancers NET-CAGE characterizes the dynamics and topology of human transcribed cis-regulatory elements Lessons from eRNAs: understanding transcriptional regulation through the lens of nascent RNAs Enhancer RNA profiling predicts transcription factor activity Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus Promoter capture Hi-C-based identification of recurrent noncoding mutations in colorectal cancer RIC-seq for global in situ profiling of RNA–RNA spatial interactions Transcriptome engineering with RNA-targeting type VI-D CRISPR effectors Wessels, H.-H. et al. Prediction of on-target and off-target activity of CRISPR–Cas13d guide RNAs using deep learning. Nat. Biotechnol. 1–10 https://doi.org/10.1038/s41587-023-01830-8 (2023) Annexin A13 promotes tumor cell invasion in vitro and is associated with metastasis in human colorectal cancer Elevated NIBP/TRAPPC9 mediates tumorigenesis of cancer cells through NFκB signaling NIK-and IKKβ-binding protein promotes colon cancer metastasis by activating the classical NF-κB pathway and MMPs The emerging role of ISWI chromatin remodeling complexes in cancer CHRAC1 promotes human lung cancer growth through regulating YAP transcriptional activity Transcription factors interact with RNA to regulate genes An oncogenic super-enhancer formed through somatic mutation of a noncoding intergenic element Selective gene dependencies in MYCN-amplified neuroblastoma include the core transcriptional regulatory circuitry Core transcriptional regulatory circuit controlled by the TAL1 complex in human T cell acute lymphoblastic leukemia Predicting master transcription factors from pan-cancer expression data Evolutionarily conserved elements in vertebrate Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells Programmable RNA targeting with the single-protein CRISPR effector Cas7-11 c-Myc is essential to prevent endothelial pro-inflammatory senescent phenotype deepTools2: a next generation web server for deep-sequencing data analysis Robust rank aggregation for gene list integration and meta-analysis Promoter of lncRNA gene PVT1 is a tumor-suppressor DNA boundary element Transcriptional regulation by promoters with enhancer function Hart, S. et al. Precise RNA targeting with CRISPR–Cas13d. Nat. Biotechnol.1–11 https://doi.org/10.1038/s41587-025-02558-3 (2025) Rational design of highly active sgRNAs for CRISPR-Cas9 – mediated gene inactivation and analysis of arrayed and pooled CRISPR genome-editing experiments High-throughput screens of PAM-Flexible Cas9 variants for gene knockout and transcriptional modulation Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening HiC-Pro: an optimized and flexible pipeline for Hi-C data processing Identification of significant chromatin contacts from HiChIP data by FitHiChIP Far-red fluorescent tags for protein imaging in living tissues StayGold variants for molecular fusion and membrane-targeting applications Download references We thank the entire Sanjana laboratory for support and advice Huang for advice and feedback on enhancer analyses and the New York University (NYU) Biology Genomics Core for sequencing resources is supported by the NIH/National Human Genome Research Institute (DP2HG010099 the NIH/National Cancer Institute (NCI) (R01CA279135 and R01CA218668) the NIH/National Institute of Allergy and Infectious Diseases (NIAID) (R01AI176601) the NIH/National Heart Lung and Blood Institute (R01HL168247) the National Science Foundation (EAGER 2409037) the Melanoma Research Alliance and the Sidney Kimmel Foundation the MacMillan Center for the Study of the Noncoding Cancer Genome These authors contributed equally: Christina M performed subsequent experiments and analysis assisted with transcription factor analysis is an adviser to Qiagen and a co-founder and adviser of TruEdit Bio and OverT Bio ‘The remaining authors declare no competing interests Nature Communications thanks the anonymous reviewers for their contribution to the peer review of this work Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41467-025-56568-5 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing: Cancer newsletter — what matters in cancer research Antoine Guldner is back with the Montreal Impact Academy as the head of performance culture Guldner will continue to develop the players’ mental skills and abilities He will accompany players throughout their journey by helping them develop performance habits and by helping them understand the situations they face is to help young players at the club progress and integrate into the first team,” said Antoine Guldner “There are specific abilities that can help them become professional players and my goal is to help our kids understand and gain these abilities I’m lucky to have had success with the Academy and the first team It’s interesting that the kids from the Academy can benefit from these experiences.” Guldner played soccer at the highest level until the age of 18 (U17 and U19 National Championship with Castelnau/Le Cres) before getting a bachelor’s degree in physical and sports education He then continued his studies to earn a master’s degree specializing in mental strength and sports performance coaching at UFR STAPS in Montpellier “What I learnt in university is important,” added Guldner “These years of studying gave me the scientific knowledge and the experience necessary to work efficiently It’s equally essential to know the club well and to understand the reality of a high-level league like MLS to help young players in their development and every member of our personnel has different skills and responsibilities that compliment each other Our goal is to have a professional environment that maximizes the progression of our young players It’s very motivating to participate in the Academy’s development and contribute to the overall success of the club.” Please enable JS and disable any ad blocker Please upgrade your browser to improve your experience Discover the flavourful secrets of the world-famous crescent-shaped pastry Whether you’re walking into your neighbourhood bakery or visiting a popular cafe the one pastry that you will always encounter is the croissant fragrant charms.This crescent-shaped delight is considered integral to the cultural heritage of the French “Croissant” comes from the word for “crescent” This describes the pastry created from rolling and folding buttery dough over and over into a thin sheet that is then cut and rolled into its iconic shape which fully forms as it bakes into a tempting golden brown.Alan Davidson no mention of croissants has been found in French recipes from before the 20th century But the croissant’s origins have been documented since 1838 or 1839 as being inspired by “Kifli” or “Kipfel” These are traditional breads made from plain flour that may contain nuts or similar ingredients which bakers form into crescent shapes before baking Breads like this were very common and were called different names across Central Europe They also pre-date the Viennese-style baked goods (Viennoiserie) from Austria.But how are the French involved in all this There is evidence that it all began when August Zang opened the Boulangerie Viennoise on Richelieu Street in Paris It is said that his Viennese breads and baked goods were so famous that he inspired the French bakers to imitate his crescent-shaped breads which turned into the world-famous croissant we know today to reveal their secrets to making and enjoying this pastry “What makes a good croissant is the contrast between the crunchiness of its crust and the soft, airy feel of its insides, with a good buttery taste,” explains Tournemolle, who reaped his rewards from hours of inventive baking and dessert making at such famous French restaurants as Le Laurent (One MICHELIN Star, MICHELIN Guide Paris) a great croissant should be flaky outside and soft inside,” shares Chef Paper revealing the secrets and knowledge she learned from culinary school in the South of France “The delicate layers inside should not stick together and it should be baked until golden brown and a buttery fragrance fills the air.” The not-so secret trick to making croissants The two pastry chefs share the same secret to fabulous baked goods: quality High-quality ingredients are half the battle won Every step affects the taste and texture of the croissant,” adds Chef Paper.“The choice of butter is the most important Butter on the drier side and richer in fat content allows the puff pastry to be layered and crunchy,” explains Chef Tournemolle this French baking guru emphasises having the correct temperature temperature control of the ingredients and the environment plays an equally important role It needs to be precisely on point for the dough to be perfectly executed.” Chef Paper prefers dipping croissants in hot chocolate and she is a happy camper with just a good plain croissant he enjoys having a croissant for breakfast with coffee and freshly squeezed orange juice even with all the flavours and fillings available After nearly two decades of polishing her prose for Marie Claire this Bangkokian finds herself as the Digital Editor of the MICHELIN Guide Thailand and Vietnam She enjoys exchanging cultural views over a Gin Rickey or getting lost in the mysteries of an Agatha Christie novel Whether it’s your first time in a fine dining restaurant or your fiftieth these tips from our seasoned MICHELIN Guide Inspectors will help you get it right — your way From creamy egg tarts to indulgent French toast these Hong Kong institutions combine local ingredients with Western influences growing focus on health and sustainability and chefs constantly pushing the boundaries of plant-based cuisine Taiwan has become a true paradise for vegetarian dining From innovative ingredients to creative techniques the diverse and exciting options make plant-based eating not just a lifestyle but a delightful part of the travel experience here rice vermicelli and a tangy dipping sauce—bun cha has won hearts and taste buds around the world We take you on a journey to explore its origins and MICHELIN-approved spots to enjoy this iconic dish preparing Lunar New Year snacks – and bonding with family while cooking – has been an invaluable component of our celebratory ritual Executive chef Li Yuet Faat of Ming Court at Mong Kok shares his experience growing up and best tips for making those snacks After seven years of closely observing Taiwan's culinary landscape a MICHELIN inspector shares the touching moments he has encountered on this charming island over the past years Restaurants showcasing the diversity of Tokyo Surprise Mum on Mother's Day with a fresh dozen of these iconic Chocolate Lavender Tarts from three-MICHELIN-star London restaurant CORE by Clare Smyth Diners are looking to enhance their dining experience with low ABV cocktails and wines with less buzz Restaurant and culinary experts from around the region discuss the up-and-coming food trends for 2020 Executive Chinese chef Daniel Cheung of Shang Palace in Hong Kong shares his recipe for these traditional Chinese New Year goodies that represent laughter and happiness Opinion leaders of the F&B industry across Asia weigh in on the top trends of the past year savoury beverages from around the world to shake up your drinking options Non-members can add the privileges at checkout through our 30 day free trial By continuing I accept the Terms & Condition and Privacy Policy. I would like to receive Newsletter from MICHELIN Guide Save lists of your favorite restaurants & hotels Whether you\u2019re walking into your neighbourhood bakery or visiting a popular cafe fragrant charms.This crescent-shaped delight is considered integral to the cultural heritage of the French \u201cCroissant\u201d comes from the word for \u201ccrescent\u201d But the croissant\u2019s origins have been documented since 1838 or 1839 as being inspired by \u201cKifli\u201d or \u201cKipfel\u201d Chef Paper prefers dipping croissants in hot chocolate, a habit she picked up in France, and she is a happy camper with just a good plain croissant. As for Tournemolle, he enjoys having a croissant for breakfast with coffee and freshly squeezed orange juice. And he agrees with Paper that, even with all the flavours and fillings available, a simple, classic plain croissant is the best. This website is using a security service to protect itself from online attacks. 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If you liked this story, share it with other people. two sons of Theodore Roosevelt (Teddy Junior and Kermit) led an expedition that killed a barking deer which has left scientists puzzled for over 80 years At first scientists believed it to be a distinct species of muntjac and named it Roosevelts’ muntjac (Muntiacus rooseveltorum) however that designation was soon cast into doubt with some scientists claiming it was a specimen of an already-known muntjac or a subspecies The problem was compounded by the fact that the animal simply disappeared in the wild No one ever documented a living Roosevelts’ muntjac again—until now “The Roosevelts’ barking deer…is one of the most poorly known mammal species in the world Since it was described as a new species in the early 20th century…no records have been reported until its rediscovery in Laos [in 1999],” write a scientific team headed by Min Le with Hanoi University of Science in a new paper in Conservation Genetics that has finally solved the mystery of Roosevelts’ muntjac All trace of Roosevelts’ muntjac vanished until 1999 when researchers found hunting trophies of the animals in Laos—though still no living animal They conducted molecular studies on these specimens which pointed to the fact that Roosevelts’ muntjac was indeed a distinct species the taxonomic status of this species was thrown in dark again as experts thought that the 1999 study was not convincing enough (using only mitochondrial DNA and so few samples…),” Le told mongabay.com this species could not be distinguished from other closely related species such as Truong Son muntjac primarily because so few specimens were available.” and 85 years since the Roosevelt expedition bagged the original specimen Le and his team have made a surprising discovery: Roosevelts’ muntjac Examining hunting trophies of muntjacs in Vietnam’s Pu Hoat and Xuan Lien Nature Reserve the team found uncanny similarities to the Roosevelt specimen still housed in the Field Museum in Chicago “This was totally unexpected,” Le said I thought I saw a new species of muntjac in Vietnam But after carefully compared our data with those from the type specimen we confirmed that it is the Roosevelts’ barking deer We were all very happy about the finding.” Le and his team conducted thorough genetic testing on their specimens which proved once again that Roosevelts’ muntjac is a distinct species “We sequenced more mitochondrial and nuclear genes of new samples We reconstructed phylogenetic relationships of the Roosevelts’ muntjac with its relatives Our genetic data reaffirmed that this species represents a distinct evolutionary lineage closely related to Truong Son muntjac in the region and leaf muntjac in Myanmar,” explained Le But this wasn’t the only breakthrough in the search for the mystery muntjac While Le and his team were conducting the genetic analyses another team succeeded in capturing the first photos of Roosevelts’ muntjac ever “The photos are very significant because they show that the species still live in the area (no one ever saw it alive since 1929!) and that there is still a chance to save it,” said Le even as Roosevelts’ muntjac is rediscovered—and confirmed as a distinct species in its own right—it may be on its way out “Hunting has been escalating in [Pu Hoat and Xuan Lien Nature Reserve],” the scientists write adding that in 1999 they “documented hundreds of traps being deployed by local people in Xuan Lien Nature Reserve.” the primary forests on which Roosevelts’ muntjac depends only cover about 10 percent of the reserves and are falling fast is for researchers to conduct surveys of Roosevelts’ muntjac to determine its population size conservationists will be able to draft an action plan “We need to act fast before it is too late to save this species,” he said Scientists are only beginning to unravel the muntjac family Generally around 12 species are recognized but shockingly—for good-sized mammals—half of these were discovered after 1980 five distinct species were uncovered in the 1990s alone a decade that might be described as muntjac taxonomic madness five (including Roosevelts’) are still categorized as Data Deficient by the IUCN Red List meaning scientists don’t have enough data to know if they are threatened or not three species are listed as Endangered and one as Vulnerable barking deer—so-called for their distinct bark-like calls—are imperiled by overhunting Several Amazonian tree frog species discovered, where only two existed before (03/18/2014) We have always been intrigued by the Amazon rainforest with its abundant species richness and untraversed expanses Despite our extended study of its wildlife new species such as the olinguito (Bassaricyon neblina) a bear-like carnivore hiding out in the Ecuadorian rainforest are being identified as recently as last year the advent of efficient DNA sequencing and genomic analysis has revolutionized how we think about species diversity scientists can examine known diversity in a different way revealing multiple ‘cryptic’ species that have evaded discovery by being mistakenly classified as a single species based on external appearance alone Scientists discover new whale species (02/14/2014) Beaked whales are incredibly elusive and rare little-known to scientists and the public alike—although some species are three times the size of an elephant beaked whales have been recorded plunging as deep as 1,800 meters (5,900 feet) for over an hour Few of the over 20 species are well-known by researchers but now scientists have discovered a new beaked whale to add to the already large group: the pointed beaked whale (Mesoplodon hotaula) Scientists make one of the biggest animal discoveries of the century – a new tapir (12/16/2013) In what will likely be considered one of the biggest (literally) zoological discoveries of the Twenty-First Century scientists today announced they have discovered a new species of tapir in Brazil and Colombia hidden from science but known to local indigenous tribes is actually one of the biggest animals on the continent although it’s still the smallest living tapir the scientists have named the new tapir Tapirus kabomani after the name for ‘tapir’ in the local Paumari language: Arabo kabomani Scientists discover new cat species roaming Brazil cats are some of the most well-studied animals on Earth but that doesn’t mean these adept carnivores don’t continue to surprise us Scientists have announced today the stunning discovery of a new species of cat Looking at the molecular data of small cats in Brazil researchers found that the tigrina—also known as the oncilla in Central America—is actually two separate species The new species has been dubbed Leopardus guttulus and is found in the Atlantic Forest of southern Brazil while the other Leopardus tigrinus is found in the cerrado and Caatinga ecosystems in northeastern Brazil World’s most cryptic feline photographed in logging concession (11/04/2013) The bay cat is arguably the world’s least-known member of the cat family (Felidae) Although first described by scientists in 1874 no photo existed of a living specimen until 1998 and a wild cat in its rainforest habitat wasn’t photographed until five years later scientists with Zoological Society of London (ZSL) and Imperial College London were taken aback when their remote camera traps captured numerous photos of these elusive cats hanging out in a commercial logging concession in Sabah Armored giant turns out to be vital ecosystem engineer (10/24/2013) The giant armadillo (Priodontes maximus) is not called a giant for nothing: it weighs as much as a large dog and grows longer than the world’s biggest tortoise many people in its range—from the Amazon to the Pantanal—don’t even know it exists or believe it to be more mythology than reality This is a rare megafauna that has long eluded not only scientific study undertaking the world’s first long-term study of giant armadillos has allowed intrepid biologist to uncovered a wealth of new information about these cryptic creatures Not only has Desbiez documented giant armadillo reproduction for the first time but has also discovered that these gentle giants create vital habitats for a variety of other species Yeti may be undescribed bear species an ape-like creature that walks upright and roams the remote Himalayas may in fact be an ancient polar bear species according to new DNA research by Bryan Sykes with Oxford University Sykes subjected two hairs from what locals say belonged to the elusive Yeti only to discover that the genetics matched a polar bear jawbone found in Svalbard Norway dating from around 120,000 (though as recent as 40,000 years ago) The “fortress conservation” model is under pressure in East Africa as protected areas become battlegrounds over history and global efforts to halt biodiversity loss Mongabay’s Special Issue goes beyond the region’s world-renowned safaris to examine how rural communities and governments are reckoning with conservation’s colonial origins and trying to forge a path forward […] Part of the 2006 generation, Joan Tincres, Aladji Bamba and Dan Sinaté were part of last season’s Coupe Gambardella adventure, which ended in a victory in the final for the Rouge et Blanc spearheaded by Frédéric Barilaro and Manu Dos Santos against Clermont (4-2) having joined last summer from Olympique Lyonnais Aladji Bamba has joined AS Monaco’s Academy in the summer of 2021 who is capable of playing as a box-to-box midfielder is one of the players most used by Frédéric Barilaro with the U19s making more than twenty appearances (U19 Championship and Gambardella Cup) After winning the Coupe Gambardella last season the midfielder went on to play five games as a first-team regular Thanks to his performances and consistency in the diagonal shirt Aladji Bamba was called up to the U18 national team Along with Joan Tincres and Saïmon Bouabré he won the Limoges International Tournament last September Aladji has signed a contract until June 2026 Dan Sinaté born in Chambray-lès-Tours (Centre Val-de-Loire) joined AS Monaco’s Academy in the summer of 2021 Dan was a starter in the victorious Coupe Gambardella final including the U19 championship and the Coupe Gambardella His performances for the Rouge et Blanc led to him being called up to the French U16 team Dan Sinaté took part in the Euro U17s last May reaching the final alongside Joan Tincres and Saïmon Bouabré Fodé Sylla 🇫🇷🇬🇳(2006) with the opener!Dan Sinaté 🇫🇷🇲🇱(2006) with the assist!#U17Euro pic.twitter.com/StmWdnLQQR — Football Report (@FootballReprt) May 20, 2023 Joan Tincres the 17-year-old from Mantes-la-Jolie joined AS Monaco from FC Mantois in the summer of 2021 he stands out for his skill in front of goal his speed and his team spirit (13 goals in 15 games in all competitions this season) Joan Tincres made a name for himself in the Coupe Gambardella starting four games and playing a key role in the Rouge & Blancs’ success He scored in the round of 16 against FC Rouen (3-1) and repeated the feat against Pau FC in the semi-final (2-0) and in the final against Clermont Foot 63 (4-2) Le festival de Joan Tincres qui a permis aux Monégasques de basculer en tête à la pause ✊ 1⃣-2⃣ #CF63ASM | #MadeInLaDiagonale pic.twitter.com/swT15loMpj — AS Monaco 🇲🇨 (@AS_Monaco) April 29, 2023 Joan Tincres is also a regular member of the French U17 and U18 teams (15 matches played) He took part in the Euro U17 in Hungary last May (finalist) the Limoges international tournament in September (winner) and the U17 World Cup in Indonesia in September (finalist) ties him down to AS Monaco until June 2027 An arrival from Olympique Lyonnais in the summer of 2023, Amal Boura is continuing his adventure with the Red and Whites After playing more than 20 games in the U17 league with Les Rhodaniens last season Amal Boura has already taken part in around 15 matches in the U19 league and the Coupe Gambardella this season Amal Boura scored a goal in the 2-1 win over OGC Nice and provided two assists in the 4-0 win over AC Ajaccio AS Monaco and Sorare extend their partnership until 2027 Mizuno joins forces with AS Monaco as the official technical equipment partner Martin van Kan brings with him over three decades of experience in the global hospitality industry having held various leadership roles with InterContinental Hotels Group (IHG) Martin's most recent role was as Area General Manager United Kingdom for IHG directing 10 managed IHG hotels and being a member of the UK&I Leadership Team he held several area general manager positions Martin's extensive background in the industry including successful hotel pre-openings and repositioning will help drive Jadranka Turizam's continued growth and excellence Jadranka Turizam operates the Lošinj Hotels & Villas Luxury Collection featuring boutique and ultra-luxury properties as well as the Classic Collection of four-star properties all located in the heart of Lošinj's healing climate and a full range of facilities ideal for an intimate getaway Jadranka Turizam operates the Camping Cres & Lošinj brand managing four campsites on the islands of Lošinj and Cres and better positioning in the global market Jadranka Turizam continues to elevate the island of Lošinj as a top luxury destination for discerning travelers worldwide The changing landscape towards more stringent environmental standards and regulations means new challenges on the quality and performance of our marine fuels We at TotalEnergies Marine Fuels are ready and equipped to provide innovative and high-quality products for the marine industry Our Research and Technical teams work in the Centre de Recherche de Solaize (CReS) Backed by years of knowledge in the formulation of fuels and lubricants our researchers work closely with the Refining Trading and Marketing divisions of TotalEnergies​ to anticipate any specification changes while honing our expertise in fuel blendstocks and fuel blending Our highly skilled team of experts have been developing programs to deep-dive into the suitability and reliability of various formulations and their skills enable them to deliver high-quality expertise to our customers They are also members of several international technical committees and standardisation organisations The Guadeloupe born full-back (177cm) who completed his medical at the club scored 3 goals and added two assists last season in 32 appearances during a loan spell with second division side Paris FC.  told the club’s official website: “I am very excited to be joining Maccabi Tel Aviv I always heard good things about Israel and the city of Tel Aviv I am here to win titles and can’t wait to start working with my new teammates and play in front of the Maccabi fans at a stadium famous for its’ atmosphere”.     added: “We are pleased to have secured Yvann as he is exactly the profile of attack minded defender that we were looking for We are all looking forward to seeing Yvann in Yellow in the coming weeks and him playing his part in the season ahead Macon made his first footballing steps at Solidarite-Scolaire before moving to France where he joined Castelnau Le Cres In 2017 he joined Dunkerque where he made his senior debut at the age of 19 and a total of 21 appearances including promotion to the second division In January 2020 Macon was bought by Sait-Etienne where he reached the French Cup Final at the end of the season Macon scored 4 goals in 48 appearances for Saint-Etienne before spending the 2022/23 season on loan with Paris FC Macon made 3 appearances for the Guadeloupe U20’s and has 2 appearances for the French U21’s Sign up for FREE in 2 easy steps to gain access to all online content and our newsletter: We are excited to welcome you to Maccabi Tel Aviv FC online please confirm your email address by opening the link in the email we sent you when you signed up In the email you will find your membership number In order to complete your registration please sign in Your spot/s will be saved for 10 minutes in order for you to be able to complete the registration A code was sent to your mobile phone please click on it in order to confirm your registration And it has been without a head since its former director died suddenly in April in a New York hotel room This article appeared in the Europe section of the print edition under the headline “A campus tale in Paris” Discover stories from this section and more in the list of contents George Simion will face Nicusor Dan, a mainstream candidate, in a run-off There are five luxuries it can no longer feasibly afford Friedrich Merz’s career is one of unforced errors and puzzling missteps. But he is serious about Europe Both Donald Trump and Ukraine’s diplomats will consider it a success Montpellier Méditerranée Métropole provides the public drinking water service : it is the organizing authority that defines the strategy and programs investments deducted from the user's drinking water bill 3 operators provide this public service in the region: Montpellier Méditerranée Métropole is drawing up and piloting the Schéma Directeur d'Eau Potable 2015-2035 which provides for €195 million in investments the project management of which is the responsibility of the Régie des Eaux Construction of a drinking water treatment unit on the VALEDEAU site rehabilitation of more than thirty water reservoirs reinforcement of the drinking water supply to several communes These numerous operations help to secure the territory's drinking water supply and anticipate future needs linked to demographic growth pipe maintenance and renewal work is also carried out every year to perpetuate the network and reduce water losses to a minimum La Régie des EauxRue Font Froide 34090 MontpellierCustomer service: 0 969 323 423 (7j/7 et 24h/24) VEOLIA Eau765 rue Henri Becquerel 34965 Montpellier09 69 32 35 52 (8am-7pm Monday to Friday and 9am-12pm Saturday) Eau du Bas Languedoc12 Route de Bessan 34 340 Marseillan / ZAC La Barthe 34660 Cournonterral0 977 408 465 (8am 7pm Monday to Friday and 8am 1pm Saturday)In case of emergency: 0 977 428 465eaudubaslanguedoc@serviceclient.toutsurmoneau.fr The water that comes out of your tap is taken from the natural environment pumped by drilling into underground aquifers or taken at the surface from watercourses protection perimeters are installed and regular checks carried out The main resource in our territory is the Lez spring, which provides the vast majority of the drinking water supply for the population of the 13 communes located within the Régie des Eaux. Other resources are drawn on to cover and secure the entire population's water needs notably water from the Canal du Bas-Rhône Languedoc (BRL) as well as local boreholes such as those in Grabels or Villeneuve-lès-Maguelone Inhabitants of the communes served by the Garrigues Campagne syndicate are supplied by local boreholes including the most important of these the Crouzette catchment in Castelnau-le-Lez the inhabitants of the 9 communes served by the Bas Languedoc syndicate are supplied mainly by water pumped from the Hérault alluvial aquifer and by water from the Canal du Bas-Rhône Languedoc (BRL) The water taken must be treated to make it fit for consumption with just chlorine disinfection when the water collected is already of good quality in which case a water treatment plant is required Montpellier Méditerranée Métropole has its own treatment plant: the François ARAGO plant the water is conveyed through closed piping networks It is stored in reservoirs that are generally located high up (underground basins on hilltops or water towers) and then conveyed to its final destination through a complex distribution system equipped with valves and regulation devices The Lez spring supplies drinking water to 74% of the population of the Metropole's 31 communes in an area of limestone rock several tens of millions of years old conducive to the infiltration and storage of rainwater thanks to the many fissures that run through it It has been exploited since the 18th century thanks in particular to the famous 19 km aqueduct built by the engineer Pitot linking the spring to the Peyrou reservoir The quality of the water from the Lez spring is subject to regular controls and in-depth monitoring it has no chronic pesticide contamination and is of good chemical and bacteriological quality as is often the case in this type of geological zone (known as a karstic aquifer) the vulnerability of the Lez spring is high and Montpellier Méditerranée Métropole is particularly vigilant about preserving it The water from the Lez spring is captured by means of 3 pumping units consisting of two submersible pumps in series operating at variable speed and delivering from 600 to 1,000 l/s The water levels measured at the Lez spring fluctuate with the seasons and especially with periods of intense pumping (during the summer) and periods of recharge (rainfall When the population's needs are high (summer) this involves pumping flows higher than the natural flow of the underground conduit but are naturally replenished during autumn rains the pumped flow is lower than the natural flow This active management is thus sustainable and preserves this water resource essential to the life of our territory by Montpellier Méditerranée Métropole to increase knowledge of how the Lez spring functions improve its management and assess its potential in the years to come the Lez - GMU (Gestion Multi-Usage) project the Département de l'Hérault and the public laboratory HSM (Hydro-Sciences Montpellier) has enabled various simulations to be carried out according to different scenarios incorporating climate change and demographic developments in the area to ensure the potential of the water resource and its long-term preservation Drinking water distribution is vital to the region guaranteeing the lives of its inhabitants and the sustainability of its development Yet the resource is becoming increasingly scarce is sometimes compromised in the face of social injustice Montpellier Méditerranée Métropole is mobilizing around these 2 issues of the future we can work towards sustainable use of water resources Montpellier and 16 other communes* in the Metropole received the "water-efficient commune" label created in 2021 by the Agence Locale de l'Énergie et du climat (ALEC) de Montpellier Métropole This label illustrates the many actions implemented in 2021 by these communes in favor of reasoned management of water resources For more information, visit the ALEC website whose aim is to raise awareness of energy and water management develop renewable energies and provide technical support for project developers within the scope of the Régie des Eaux (i.e an eco-solidary water pricing system will combine user responsibility in their consumption with the introduction of a progressive tiered tariff with the introduction of direct assistance in paying their bills Marie Amélie Tufano ©Un peu de douceur dans ce monde de brutes et ce sont les Sud’crés qui vous l’apportent Quoi de mieux qu’une bonne pâtisserie pour égayer une journée C’est toujours un plaisir de voir cet art se construire sous nos yeux et encore plus de pouvoir y goûter L’association les Sud’crés nous ont fait l’honneur de les déguster pour vous avant leur sortie.  On vous présente les pâtisseries de nos chefs renommés de Marseille dont on est très fiers Tout d’abord petit point sur l’association «Les Sud'crés » de Gourméditerranée Son histoire débute avec  les fondateurs Jérôme Raffaelli (pâtisserie Oh Faon !) et Vanessa Robuschi (restaurant Question de goût) Clin d’œil au Club des Sucrés lancé à Paris par Christophe Adam et Christophe Michala On compte aujourd’hui une quinzaine de pâtissiers (boutique ou restaurant) glaciers et chocolatiers de la région Les Sud’Crés participent aux événements de Gourméditerranée et se réunissent trois à cinq fois par an pour présenter leurs créations interprétant à leur manière les temps forts du calendrier pâtissier ou composant sur des thèmes qui varient au gré des saisons et des inspirations. Mais ce qu'ils prônent c’est l’esprit bon vivant et chaleureux. La présidente de l'association gourmande, Vanessa Robuschi nous explique que c’est avant tout un moment de partage et de convivialité : « Les Sud’crés c’est un regroupement de copains qui n’ont pas le temps de se voir à côté du boulot. Le but était de voir les personnalités de chacun il n'y a absolument pas d’égo ici. » En ce début d’été c’est la fraise qui est mise à l’honneur Chaque chef(fe) décline l’incontournable tarte aux fraises à sa façon et c’est pari gagné À l’honneur de ce rassemblement autour de la fraise c’est le producteur Ludovic Long qui fournit les chef(fe)s. Il joue un rôle important aux côtés de nos pâtissiers pour les guider dans la sélection de ses produits Place à la dégustation maintenant c’est la fête aux fraises  c’est l’invité d’honneur Lionel Levy chef de l’intercontinental qui nous présente son ceviche de loup à la fraise On ne sent pas le sucré de la fraise mais seulement son acidité qui ressort et se marie parfaitement bien avec le poisson Une recette fraîche et estivale pour cette saison Le plat sera disponible à la carte des brunchs de l’Intercontinental Dépêchez-vous avant que la saison des fraises ne se termine  Pour ceux qui aiment les mélanges plus surprenants on vous conseille d'opter pour la tarte sablée au parmesan et au crémeux de petit pois de la pâtisserie d’Émotion ou bien peut-être la déclinaison de la fraise dans tout ses états relevée au clou de girofle de Vanessa Robuschi Pour ceux qui aiment la fraîcheur et la légèreté, vous apprécierez la glaçe à la fraise des Glaciers Marseillais Et maintenant à vos fraises !  Marie Rebuffat ouvre sa pâtisserie sans gluten ni lactose à Marseille Découvrez les 3 pâtisseries préférées des Français former home of botanical artist Helen Burrows blossoms on the marketAlesha Capone The late botanical artist Helen Burrows created a leafy paradise at 2 Le Gallienne Cres A ‘fairytale’ Mulgrave house with a storybook-like garden created by noted botanical artist Helen Burrows awaits a new owner Named Camellia Cottage after the flowers which Burrows was best known for painting, the five-bedroom Cape Cod New Colonial-style home at 2 Le Gallienne Cres has a wraparound pergola draped in blue wisteria citrus grove and Lebanese Cedar tree also feature RELATED: Woodend: Paul Bangay’s former ‘Enchanted Gardens’ property up for sale Artists rebuild Sherbrooke Forest sanctuary ‘treehouse’ with indoor garden, waterfall window South Yarra ‘frozen botanical waterfall’ house for sale taught art at the Friends of the Royal Botanic Gardens for more than three decades Her work is held in the US-based Hunt Institute’s prestigious permanent collection National Library of Australia and international museums Buxton Wheelers Hill director Bruce Liu said the “fairytale” Camellia Cottage has dormer windows and a gabled roof and sits on a northwest facing 657sq m corner block I thought the building was like coming from a storybook,” Mr Liu said Camellia Cottage presents as a green vision Crisp white paintwork and European appliances in the kitchen Burrows’ daughter Eloise Peace said that when her mother purchased the 1966-built property 20 years ago the garden consisted of “barren dry dirt” and the house itself was “very run down” “I thought she was mad to buy the house but she loved the arts and crafts feel of it loved the tree and she wanted to be close to family,” Ms Peace said Ms Burrows transformed Camellia Cottage into a botanical gem complete with a Japanese maple grove outside the window of her former studio A window to the well-maintained trees outside Another detailed botanical artwork by Helen Burrows “So much of her catalogue of work is still growing in the garden “She sought out diverse camellia specimens to compliment the few originals on site “The ornamental grape vine on the porch goes a brilliant orange red in autumn and the wisteria blooms in October are full of cascading blue flowers.” An undercover entertaining area is protected from the elements Her mother also enjoyed making jam from the front yard’s quince trees and hung her artworks inside including one of her signature camellia prints Ms Peace said her mum built the second-storey extension in 2011 with the Cape Cod design inspired by the surrounding streets’ 1960s-era houses “She rearranged some of the original plan layout Plantation shutters in one of the five bedrooms Sit in the garden and enjoy the quiet outside Highlights include satin finish Tasmanian Oak floors 7500L of rainwater storage and internal access from the garage Each of the top floor bedrooms has an ensuite while the upper level also offers a sun-filled central retreat The house is zoned for Brentwood Secondary College and is located close to other schools Camellia Cottage has a $1.27m-$1.39m asking range and will be auctioned on July 29 MORE: REIV Quarterly Medians: Melbourne million dollar house club growing again Murrumbeena: Family builds brand new home with laundry chute the unexpected feature piece AILA awards: Ned Kelly’s childhood home in Beveridge up for heritage landscape gong third parties have written and supplied the content and we are not responsible for it completeness or reliability of the information nor do we accept any liability or responsibility arising in any way from omissions or errors contained in the content We do not recommend sponsored lenders or loan products and we cannot 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By accessing or using our platform, you agree to our Terms of Use. The train travelling between Nimes and Montpellier hit a tree that had fallen on the track at the small town of Le Cres, they said, adding that another 50 people had suffered minor injuries. PoliticsMerz fails to be elected in initial German parliament voteMerz fell short with 310 votes out of the required 316 Skin healthThe hype surrounding skincare routines“Seven steps to perfect skin” - skincare is a popular social media topic. But what really works? 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All rights reserved Here are 9 stalls that you should try in Commonwealth Crescent Market & Food Centre: Let me just say that the Braised Duck Rice ($4) at 牛车水阿婆卤鸭 hits just all the right spots The sauce had the perfect balance of savoury and sweet notes and it was more on the watery side (which I love) 牛车水阿婆卤鸭 serves a scoop of hae bi hiam (dried spicy shrimp) with its rice Address: #02-95 Commonwealth Crescent Market & Food Centre Whenever I am in Commonwealth Crescent Market & Food Centre I always make sure to buy myself half a chicken ($12) and a plate of rice ($0.50) from Henry’s Chicken Rice The couple running this stall has been serving the same consistent plate of chicken rice since 1985 While there are other options such as steamed chicken and roasted chicken I always go for the roasted option because of its glistening and addictive skin even the breast meat) is always moist and tender Remember to get yourself a portion of chilli Henry’s Chicken Rice’s chilli is so good that I am willing to call it sacred And you are only entitled to one portion of chilli for each plate of chicken rice Come early because Henry’s Chicken Rice sells like hot cakes Address: #02-84 Commonwealth Crescent Market & Food Centre Opening Hours: 9am to until sold out (usually sells out by afternoon) I have always loved wanton noodles and the Wanton Noodles ($3) at Hao Hao Noodle House really evokes nostalgia Serving old-school wanton noodles and various other noodle dishes Hao Hao is helmed by a pastry chef-turned-hawker who has been cooking wanton noodles for the past 2 decades Hao Hao Noodle House’s wanton noodles is served with a sprinkling of lard It wasn’t overly salty and I enjoyed the noodles I watched the hawker as he was cooking my wanton noodles and he is very skilful then launches it up into the air to aerate the mee kia The end product is a plate of wanton noodles with super springy & QQ noodles that is ugh Address: #02-83 Commonwealth Crescent Market & Food Centre Don’t let the young hawker behind 黄大福 fool you because he serves great and affordable Bak Chor Mee ($2.70) The sauce in the bak chor mee was a mix of chilli and vinegar which coated each strand of mee pok evenly It was perfectly cooked and was so irresistibly soft and tender It’s not the best bak chor mee that I have eaten 黄大福 definitely deserves some credit and warrants a visit Address: #02-94 Commonwealth Crescent Market & Food Centre Hong Kee Porridge has been serving the ultimate comfort food — Cantonese-style porridge— for the past 4 decades The Pork Porridge with Century Egg ($3.50) and the Fish Head Porridge ($3) I particularly loved the Pork Porridge with Century Egg; there was a fine mix of pork slices and marinated pork balls that made for a really comforting and filling bowl I spoke to the stall owner and he said that he is not getting any younger and intends to retire soon So make sure to get yourself a bowl of porridge before he closes shop Address: #02-89 Commonwealth Crescent Market & Food Centre you should snack on the curry puffs made by Milah Hokkaido Curry Puff Milah Hokkaido Curry Puff uses 100% “Wonderful Traded Flour” from Hokkaido Milah Hokkaido Curry Puff sells 4 flavours of curry puffs — Potato I decided to get all but the Sardine curry puff *TIP: The curry puffs taste the best when they are fresh out of the fryer The first batches are ready for sale at 11am.* The Mentaiko Cheese and the Cinnamon Banana Curry Puff really dazzled my tastebuds The Cinnamon Banana puff can be likened to an apple pie and the cheesiness in the Mentaiko Cheese puff was just lovely You really have to savour the puffs at their best The puffs here are really unique and the light crust of each puff is just delightful Address: #02-64 Commonwealth Crescent Market & Food Centre Facebook: https://www.facebook.com/Milah-Hokkaido-Curry-Puff-1809247769113016/ Selling just 2 burgers initially — Classic Beef Cheeseburger ($6) and Fried Chicken Burger ($5.50) — Hammee’s has been consistently experimenting with new flavours to develop a larger menu Hammee’s Premium Beef Cheeseburger ($8) has been well-received by its patrons homemade sauce and a 120g handmade beef patty The difference between the Classic Beef Cheeseburger and Premium Beef Cheeseburger is that short ribs are used in the Premium Beef Cheeseburger I actually prefer the chicken burger to the Premium Beef Cheeseburger (despite the fact that I am a fan of anything beef) The spicy kick from the cubed jalapeños was lovely and it kept me coming back for more I will probably order the spicy version again because I really enjoyed it Commonwealth Crescent Market & Food Centre Facebook: https://www.facebook.com/hammees93/ This traditional Chinese dessert stall at Commonwealth Crescent Food Centre is run by a granny who has been making desserts using the same recipes for almost half a century There are four types on offer here: green bean soup cheng tng and sweet wheat porridge – all served hot I like the sweet wheat porridge with coconut cream in it It is not too sweet and the wheat pearls come with a nice chewy bite The green bean soup is also fragrant and cooked with sago and coconut milk What better way to end your meal than with a cup of fresh & organic Avocado Milkshake ($3.50) from ASiP Fresh Cold Press Juice The avocado milkshake consists of only three premium ingredients: halved avocados They don’t even put ice into their milkshake you can request for ice to be added into your milkshake the avocado milkshake was extremely thick and creamy just like how avocado milkshakes should be But what I like best about this stall is that the auntie asks questions(e.g more gula melaka syrup etc) pertaining to your drink in order to tailor a shake to your liking Address: #02-85 Commonwealth Crescent Market & Food Centre Commonwealth Crescent Market & Food Centre has so much more to offer head over to Commonwealth Crescent Market & Food Centre for lunch or dinner today MissTamChiak.com made anonymous visits and paid its own meals at the stalls featured here Let’s build a food community that helps to update the food news in Singapore Simply comment below if there’s any changes or additional info to the cafes/stalls listed above For more food videos, check out and subscribe to our official YouTube channel Join us on our Telegram channel Site by Stitch Interactive Terms of use•Privacy policy