The Business of FashionAgenda-setting intelligence analysis and advice for the global fashion community access one complimentary BoF Professional article of your choice Receive news, offers and invites from BoFOur newsletters may include 3rd-party advertising, by subscribing you agree to the Terms and Conditions & Privacy Policy Victoria Beckham has appointed Sybille Darricarrère Lunel as its new chief executive She will be based in the brand’s London headquarters Darricarrère Lunel joins from Christian Dior Couture where she was business unit director of leather goods overseeing the category’s global product assortment She previously held leadership roles at Galeries Lafayette and The Kooples.“ I’m excited to have Sybille joining the company as CEO,” said founder and creative director Victoria Beckham smart woman with an entrepreneurial mindset and energy by my side to take us to the next level.” The appointment comes as Victoria Beckham brand continues efforts to strengthen its business. In recent years, the brand has streamlined operations, introduced new product categories and refined its pricing strategy to balance its high-end positioning with broader commercial appeal “I have always admired the brand’s feminine which empowers women,” said Darricarrère Lunel founder & managing partner of NEO Investment Partners which holds a majority stake in the business “She is the perfect partner to write the next chapter for Victoria Beckham—one of ambition Victoria Beckham Losses Shrink as Sales Soar 50% at Fashion Brand The fashion and beauty business reduced losses to £200,000 with a 52 percent sales surge to £89 million last year driven by online growth and expansion into new product areas Simone Stern Carbone is Luxury Correspondent at the Business of Fashion She is based in Zurich and Paris and covers fashion and beauty For more information read our Terms & Conditions In this riveting conversation from BoF CROSSROADS 2025 Mexican designer Carla Fernández and Tunisian entrepreneur Kenza Fourati discuss the power of craft-based fashion and how to collaborate ethically with artisans and indigenous communities The Hollywood dealmaker’s new firm is buying the powerful international art fair brand Emanuel’s company and the future of the art business With a new store concept and marketing campaign the designer is looking to widen his brand’s appeal beyond the fashion elite 4G — resellers who run an infamous New York showroom where rappers and athletes pay stratospheric prices to load up on Chrome Hearts and ‘fucking chill’ — have built a business that has doubled sales revenue each year since 2020 Victoria Beckham has appointed Sybille Darricarrère Lunel previously with Dior and Galeries Lafayette as the new chief executive officer of the London-based fashion company Darricarrère Lunel will succeed Ralph Toledano and will operate from the company’s London headquarters the founder and creative director of the brand expressed her enthusiasm about the new appointment smart woman with an entrepreneurial mindset and energy by my side I look forward to leading this company with Sybille and having her knowledge and respect for product to help us go to the next level,” Beckham stated Darricarrère Lunel brings extensive experience from her recent role as the global business unit director of leather goods at Christian Dior Couture she managed the global product assortment across five collections annually she served as the general merchandise and buying director at Galeries Lafayette covering various departments including womenswear Her earlier career includes several years in planning and merchandising at The Kooples founder and managing partner of NEO Investment Partners a significant minority stakeholder in the business makes her our perfect partner to write the next chapter for Victoria Beckham — one of ambition creativity and high profitable growth.” Darricarrère Lunel expressed her admiration for the Victoria Beckham brand and its founder “I have always admired the Victoria Beckham brand’s feminine I have a deep admiration for Victoria’s work her creative vision and her attention to detail I look forward to collaborating with the talented teams who have achieved remarkable success alongside her as we begin building exciting new chapters together,” she said has appointed Sybille Darricarrère Lunel as its new CEO Darricarrère Lunel will assume her new role at the British fashion house effective July 1 She takes over from chairman Ralph Toledano and will be based at the brand's London headquarters and intelligence immediately impressed me during our first meeting,” said Toledano “Her background and understanding of product make her the perfect CEO for our brand following the successes of recent years and with the support of our outstanding team she will significantly accelerate the growth of Victoria Beckham.” The new CEO joins from French fashion house Christian Dior Couture where she served as global business unit director for leather goods she oversaw the global product assortment across five collections per year and worked closely with teams in design Darricarrère Lunel was general merchandise and buying director at French luxury retailer Galeries Lafayette She also gained experience at the Parisian brand The Kooples she acquired a “deep understanding and knowledge of commercial strategies and retail growth as well as extensive experience in collection planning and merchandising,” according to the announcement “I have always admired the brand's feminine “I have great admiration for Victoria’s work as we embark together on exciting new chapters.” FashionUnited uses AI language tools to speed up translating (news) articles and proofread the translations to improve the end result This saves our human journalists time they can spend doing research and writing original articles Articles translated with the help of AI are checked and edited by a human desk editor prior to going online If you have questions or comments about this process email us at info@fashionunited.com We use cookies to provide our services and for analytics and marketing. To find out more about our use of cookies and how you can disable them, please see our Privacy Policy. By continuing to browse our website, you agree to our use of cookies. Click here to read our privacy policy. “I hope that we put stars in the eyes of little girls and they will dream of competing in artistic swimming,” says Paris 2024 Olympian Romane Lunel The 20-year-old was part of the history-making women’s team who matched their nation’s best-ever Olympic Team Event performance in the sport The 2023 European Games champion, who also competed as part of the Olympic duet alongside team-mate Anastasia Bayandina will compete in both disciplines at the season-opening World Aquatics Artistic Swimming World Cup in the French capital The competition, which runs from 28 February to 2 March, will be the first major event for the best artistic swimmers on the planet since the Paris 2024 Olympic Games. View this post on Instagram A post shared by Romane Lunel (@romanelunel) “Paris 2024 was magical from start to finish,” Lunel tells World Aquatics “Between the opening and closing ceremonies I only have good memories of the exceptional support from the public “(Now) I’m really looking forward to competing again and it’s really cool to be able to do it again in front of our audience They are always there (to support) and that gives us a lot of energy.” With the Aquatics Centre in St. Denis undergoing planned post-Games redevelopment work, the athletes will return to the 1924 Olympic venue, Piscine Georges Vallerey, which was used as a training based during last summer’s Games. View this post on Instagram A post shared by Aqualillies (@aqualillies) While the Olympic Games shone a sporting spotlight on the French capital two months after the Games concluded international attention turned back to Paris but with a more traditional focus on fashion For 2024’s edition of Paris Fashion Week though there was a very unique twist to French designer Christian Louboutin’s ‘Spring / Summer 2025’ show which was presented at the iconic Piscine Molitor which first opened in 1929 and possesses a historic art deco swimming pool There, the French Olympic team returned to the water for one last time, with the help of Italian World champion Giorgio Minisini, and performed a series of dazzling routines – most notably while wearing striking ‘Miss Z’ stilettos. The fashion show was created by film director and photographer David LaChapelle together with choreographer Blanca Li and was entitled ‘Paris is Louboutining’. “It was cool to be able to show that our sport can be competitive sport but also a spectacle sport,” Lunel tells World Aquatics. “We had about two weeks of rehearsals to prepare a 15-minute show and we were accomplished by Blanca Li and David LaChapelle, which went really well. “It was an amazing experience to see how these events happen from the inside. View this post on Instagram A post shared by Romane Lunel (@romanelunel) with the new regulations (in artistic swimming) so it was really good to show our sport in another form,” said Lunel before answering the question most people were asking after witnessing the performance while laughing about the team’s desire to retain the eye-catching footwear Lunel feels Paris 2024 helped “shine a light” on her sport which is traditionally “very little recognised in her homeland” and believes the athletes helped “put stars in the eyes” of young fans who were watching either in the venue or on television last summer Therefore, many who have decided to return are doing so with a new perspective and renewed motivation.  “The objective for the next few years will be to rebuild a new team and seek World medals,” reveals Lunel, who has won three European Championship honours with the national team. “This (World medals) is what we are missing, and, in the end, going to win the medal in LA (Los Angeles Olympic Games in 2028) that we didn’t get in Paris would be really incredible. Lunel continues, “Singapore (2025 World Aquatics Championships) will therefore be very important, and we hope to win a medal there.” View this post on Instagram A post shared by World Aquatics (@world_aquatics) The top performers from the first three World Cup events will qualify for the 2025 Super Final in Xi’an, China The senior international season will then conclude with the World Aquatics Championships in Singapore with the artistic swimming programme running from 18-25 July Keep visiting the World Aquatics website and social media platforms from 28 Feb to 2 March for all the latest from the Paris leg of the Artistic Swimming World Cup Tuesday | May 06 2025 | The Sheraton Grand Bangalore at Brigade Gateway has named Richard Lunel as the Rooms Division Manager of the hotel with over a decade of experience in operations brings a diverse skill set and knowledge to his new role A professional with a background in kitchen operations Lunel's varied experience allows him to comprehend the complexities of each department An alumnus of the Oberoi Centre of Learning and Development Having worked at luxury hotels within the Oberoi Group Lunel has honed his expertise in the industry His educational background includes a Master of Business Administration (Global) from Deakin University and a Postgraduate Programme in Management from the Institute of Management Technology vivek.mittal@businessworld.in, amit.bhasin@businessworld.in Subscribe to our newsletter to get updates on our latest news Register to Drapers to read ONE FREE article and a FREE digital edition Victoria Beckham has named Sybille Darricarrère Lunel who also spent five years at French luxury department store Galeries Lafayette in head of roles who took the post after Marie Leblanc's exit in October 2024 Leblanc has returned to Paris to take the top job at French luxury label Courrèges Victoria Beckham founded her eponymous brand in 2008 and stepped down as creative director in 2024 has been design director of the brand since 2022 Beckham said she was excited to have a "strong Darricarrère Lunel added: “I am absolutely delighted and thrilled to be joining Victoria Beckham as its new CEO I have always admired the brand’s feminine "I have a deep admiration for Victoria’s work as we begin building exciting new chapters together.” Toledano will continue in his role as chairman of the British luxury brand He has held the post since 2017 after French firm New Investment Partners acquired a 30% stake in the business I am sure that following the achievements of the past few years she will considerably accelerate the growth of Victoria Beckham." Victoria Beckham has experienced five years of sizeable losses, but returned to profit during the 2022 financial year after the New Investment Partners acquisition founder and managing partner of New Investment Partners there comes a moment when the right leadership unlocks its true potential makes her our perfect partner to write the next chapter for Victoria Beckham – one of ambition Tags analysis and advice to keep you updated… New Look has successfully onboarded 100% of its core supplier base onto… Marks & Spencer has reassured that all third-party brands trading on its… Get full access to all the fashion industry news and intelligence you need Browse the archive of more than 55,000 articles read the daily and weekly newsletters in full and gain priority access to Drapers events Please enable JS and disable any ad blocker You don't have permission to access the page you requested What is this page?The website you are visiting is protected.For security reasons this page cannot be displayed Victoria Beckham has appointed Sybille Darricarrère Lunel as Chief Executive Officer of her London-based fashion brand following the exit of Marie Leblanc last year Darricarrère Lunel will join Victoria Beckham on 1 July taking over from acting CEO Ralph Toledano She will be based at the brand's London headquarters Darricarrère Lunel told the publication that she has always admired the Victoria Beckham brand's "feminine She added: "I have a deep admiration for Victoria's work as we begin building exciting new chapters together." Darricarrère Lunel most recently served as Global Business Unit Director for Leather Goods at Christian Dior Couture She first joined the luxury house in 2022 as Global Head of Marketing she was General Buying & Merchandising Director at Parisian department store Galeries Lafayette across womenswear She also previously worked for The Kooples for more than five years said: "Darricarrère Lunel's entrepreneurial spirit enthusiasm and intelligence immediately spoke to me when we first met make her the perfect Chief Executive Officer for our brand." The news comes after Beckham appointed Lauren Edelman as CEO of her beauty business earlier this year who announced her departure after more than two years in the role By clicking 'Get the Newsletter' below, you agree to receive the newsletter & marketing communications from theindustry.fashion. 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Volume 14 - 2020 | https://doi.org/10.3389/fncel.2020.552213 Willin/FRMD6 is part of a family of proteins with a 4.1 ezrin-radixin-moesin (FERM) domain It has been identified as an upstream activator of the Hippo pathway and is associated with human diseases and disorders Even though Willin/FRMD6 was originally discovered in the rat sciatic nerve most studies have focused on its functional roles in cells outside of the nervous system where Willin/FRMD6 is involved in the formation of apical junctional cell-cell complexes and in regulating cell migration we investigate the biochemical and biophysical role of Willin/FRMD6 in neuronal cells employing the commonly used SH-SY5Y neuronal model cell system and combining biochemical measurements with Elastic Resonator Interference Stress Micropscopy (ERISM) We present the first direct evidence that Willin/FRMD6 expression influences both the cell mechanical phenotype and neuronal differentiation By investigating cells with increased and decreased Willin/FRMD6 expression levels we show that Willin/FRMD6 not only affects proliferation and migration capacity of cells but also leads to changes in cell morphology and an enhanced formation of neurite-like membrane extensions These changes were accompanied by alterations of biophysical parameters such as cell force the organization of actin stress fibers and the formation of focal adhesions changes in Willin/FRMD6 expression inversely affected the activity of the extracellular signal-regulated kinases (ERK) pathway and downstream transcriptional factor NeuroD1 which seems to prime SH-SY5Y cells for retinoic acid (RA)-induced neuronal differentiation to study the involvement of Willin/FRMD6 in biophysical aspects of SH-SY5Y cells ERISM is a recently developed force mapping tool that allows for imaging of cell forces with high spatial resolution Combining ERISM with biochemical measurements we discovered that manipulating Willin/FRMD6 expression levels in SH-SY5Y cells influences both biochemical and biophysical effects Reducing endogenous Willin/FRMD6 expression resulted in a change in proliferation rate and cell morphology specifically an increase in fine membrane extensions which in turn rendered the cells more susceptible to differentiation following RA stimulation These cellular changes were accompanied by changes in the forces that the cells exerted onto their environment changes in Willin/FRMD6 expression resulted in alterations of the actin cytoskeleton and the localization of TAZ; though notably these events were controlled by activation of the extracellular signal-regulated kinases (ERK) pathway These combined biochemical and physical measurements provide the first direct evidence that Willin/FRMD6 has potential roles in the development of neuronal-like cells and that these occur predominantly through the ERK signal transduction pathway Antibiotic selection was maintained for 3 weeks of post-viral transduction Lysates of cell monolayers were prepared using lysis buffer (50 mM Tris-HCl 0.1% sodium dodecyl sulfate (SDS); all reagents purchased from Sigma–Aldrich) and lysate protein concentrations measured by BCA assay Protein samples prepared with 20 μg of protein lysate and 5× loading buffer (500 mM dithiothreitol 0.25% bromophenol blue) were denatured by heating at 95°C for 5 min and separated by SDS-PAGE Proteins were then transferred in a liquid buffer (25 mM Tris base 20% (v/v) methanol) onto a PVDF membrane (EMD Millipore) using a Bio-Rad blotting module at 25 V for 90–120 min Membranes were stained with Ponceau S solution (Sigma–Aldrich) to verify effective protein transfer Membranes were blocked in either 5% skimmed dried milk in TBS-T or 5% BSA in TBS-T for 1 h at room temperature before being incubated with primary antibody diluted in blocking solution overnight at 4°C [FRMD6: Cell Signaling Technology 1:100; Phospho-p44/42 MAPK: Cell Signaling Technology 1:4,000; p44/42 MAPK (ERK1/2): Cell Signaling Technology 1:2,000; NeuroD1: Santa Cruz Biotechnology 1:500; β-actin: Sigma–Aldrich Membranes were then washed three times with TBS-T for 10 min and incubated with horseradish peroxidase (HRP)-conjugated secondary antibody diluted in blocking solution for 1 h at room-temperature (Abcam Membranes were washed with TBS-T for 15 min three times and protein bands were detected by the addition of 0.75 ml Immobilon Western Chemiluminescent HRP Substrate for 1 min Protein bands were visualized with the Fujifilm LAS-3000 imaging system and analyzed using FIJI membranes were incubated with fluorescently labeled secondary antibodies (Li-Cor) and bands detected using the Li-Cor Odyssey CLx (Li-Cor) Bands of the protein of interest were quantified using GAPDH or β-actin as a loading control A serum-free SH-SY5Y cell suspension (5 × 105 cells) was added to cell culture inserts with 8.0 μm pores (BD Biosciences Growth media supplemented with 20% serum was added to the lower chamber The bottom side was stained with 0.3% crystal violet (Sigma–Aldrich) The number of cells on the lower surface of each chamber was counted from images acquired using a Zeiss Axiovert 40CFL microscope The area of cells was measured from phase-contrast images taken 24 h after seeding the cells on ERISM substrates the outlines of the cells were drawn by hand using the FIJI software package and the area of the marked region was calculated Cell elongation factors were calculated from the same images by measuring the longest (l) and shortest (s) diameter of a cell and calculating the elongation as (1 – s/l) Cell membrane extension lengths were determined by hand using the FIJI software package. All cells possessing at least one extension with a length at least twice the cell body diameter were classified as a neurite-like extension-bearing cell (“pre-neurite”; Popovics et al., 2013) Results were expressed as the percentage of fine extension bearing cells in the entire cell ensemble cells were either fixed directly on ERISM substrates or coverslips Fixation was achieved with 4% paraformaldehyde (Alfa Aesar) in PBS at room temperature for 20 min Cells were washed twice with 0.05% Tween-20 in PBS (Alfa Aesar) permeabilized with 0.1% Triton X-100 (Alfa Aesar) in PBS for 3 min and blocked for 30 min with 1% BSA (Carl Roth) in PBS Fixed cells on ERISM chips were simultaneously incubated with primary antibodies for vinculin (Merck Millipore 1:100) in blocking solution for 60 min at RT incubated with the secondary antibodies (Sigma–Aldrich 1:400) and TRITC-conjugated phalloidin (Merck Millipore 1:500) in blocking solution for 45 min at RT 1:1,000 in blocking solution for 3 min at RT) Fixed cells on coverslips were incubated with a primary antibody for neuroD1 (Santa Cruz Biotechnology incubated with a secondary antibody (Jackson ImmunoResearch 1:1,000 in PBS for 30 min at RT) and washed again Antifade Mountant with DAPI (Invitrogen; P36935) was used to visualize cell nuclei SH-SY5Y cells were plated on the ERISM substrate or glass coverslip and incubated for 24 h Cells then underwent two gentle washes of PBS to remove any excess serum leftover from the growth media before the addition of SH-SY5Y differentiation media [DMEM:F12 Fresh differentiation media was added to the cells every 2 days for a period of 7 or 8 days Differentiated cells were defined as cells with neurites that were longer than 40 μm Knock-down of Willin/FRMD6 affects proliferation (A) Quantitative Western blot analysis of Willin/FRMD6 expression in control (shScr) and Willin/FRMD6 knock-down (shWillin) SH-SY5Y cells Means were calculated from three independent experiments GAPDH serves as a loading control of the lysates (B) qPCR analysis of Willin/FRMD6 mRNA expression in shScr and shWillin cells Means and SEM (error bars) were calculated from two independent experiments each of which was conducted in triplicates (C) Growth curve of shScr and shWillin cells Means (horizontal lines) and SEM (error bars) were calculated from three independent experiments (D) Assessment of migration of shScr and shWillin cells in Boyden chambers after 24 h Means (horizontal lines) and SEM (error bars) were calculated from two independent experiments and Fourier-filtered ERISM maps (lower row) of representative shScr (left column) and shWillin (right column) cell Comparison of (F) volume by which cells indent into the ERISM substrate and (H) cell elongation of shScr and shWillin cells Each data point represents the measured value for one cell taken from four (F) and two (G,H) independent experiments Groups were compared using Student’s t-test; ***p ≤ 0.001 A decrease in Willin/FRMD6 expression increased proliferation (mean cell number after 8 days ± SEM: shScr: 22,600 ± 600, n = 9; shWillin: 46,700 ± 1,700, n = 9; Student’s t-test: p ≤ 0.001) and migration capacity (mean number of migrating cells ± SEM: shScr: 331 ± 9, n = 6; shWillin: 417 ± 8, n = 6; Student’s t-test: p ≤ 0.001; Figures 1C,D) of SH-SY5Y cells The number of focal adhesion contacts and the magnitude of substrate twisting again indicating a reduction in cell force upon Willin/FRMD6 knockdown Knock-down of Willin/FRMD6 alters vinculin expression (A) Representative phase-contrast images (upper row) vinculin (green) and DNA (blue; middle row) and TAZ (lower row) of control (shScr) and Willin/FRMD6 knock-down (shWillin) SH-SY5Y cells Comparison of (B) the number of focal adhesions per cell (C) the mean area of a single focal adhesion and (E) the nuclear/cytosolic TAZ ratio in shScr and shWillin cells (C) and (E) represents the measurement value for one cell taken from two independent experiments (D) Quantitative Western blot analysis of vinculin expression in shScr and shWillin cells Correlation between the ratio of nuclear to cytoplasmic TAZ concentration and (G) the volume by which cells indent into the ERISM substrate for shScr (green dots) and shWillin (yellow dots) SH-SY5Y cells Groups were compared using Student’s t-test; **p ≤ 0.01; ***p ≤ 0.001 Willin/FRMD6 over-expression results in a general reversal of the findings observed for knock-down cells and activate the mechanical activity of SH-SY5Y cells (A) Quantitative Western blot analysis of Willin/FRMD6 for control (Vector) and Willin/FRMD6 overexpressing (Willin) SH-SY5Y cells β-actin serves as a loading control of the lysates (B) qPCR analysis of Willin/FRMD6 expression in Vector and Willin cells (C) Growth curve of Vector and Willin cells Means and SEM (error bars) were calculated from three independent experiments (E) Representative phase-contrast images (upper row) vinculin (green) and DNA (blue; middle row) and TAZ (lower row) of Vector and Willin SH-SY5Y cells and (I) volume by which cells indent into the ERISM substrate for Vector and Willin cells Each data point represents the value measured for one cell Groups were compared using Student’s t-test; ns: p > 0.05; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 Knockdown of Willin/FRMD6 in SH-SY5Y cells activates the ERK1/2 pathway and elicits differentiation into a neuronal phenotype by activation of the transcriptional factor NeuroD1 Quantitative Western blot analysis of the levels of pERK1/2 relative to ERK1/2 (A) in control (shScr) and Willin/FRMD6 knock-down (shWillin) SH-SY5Y cells (B) in control (Vector) and Willin/FRMD6 overexpressing (Willin) SH-SY5Y cells (C) in SH-SY5Y wild type cells treated with either DMSO or retinoic acid (RA) and (D) in SH-SY5Y wild type cells treated with either DMSO or U0126 Means in (A) to (D) were calculated from three technical repeats Comparison of (E) percentage of fine extension-bearing cells and (F) volume by which cells deform the ERISM substrate (each data point represents the value measured for one cell) for shScr cells treated with DMSO and shWillin cells treated with either DMSO or U0126 for 24 h In (E) means were calculated from two independent experiments conducted in triplicate In (F) each data point represents the value measured for one cell (G) Percentage of differentiating shScr and shWillin cells quantified from bright-field images of cells treated with 10 μM RA along with either DMSO or 10 μM U0126 for 4 days Quantification of the levels of NeuroD1 in shScr and shWillin cells from (H) Western blot analysis and (I) qPCR analysis Means in (H) and (I) were calculated from two independent experiments conducted in triplicate (J) Immunofluorescent imaging of shScr (upper row) and shWillin (lower row) cells for NeuroD1 (left column) and DNA (right column) (K) Quantification of extension bearing cells from bright-field images for shScr cells treated with 10 μM RA for 1 (L) Development of the volume by which cells indent into the ERISM substrate over 1 week during RA-initiated (10 μM) neuronal differentiation of shScr cells Data points represent means measured for ni individual cells; shaded areas represent SEM (Day 1: nshScr = 16 To further investigate the effect of Willin/FRMD6 knockdown on exertion and transmission of cell force, shScr and shWillin cells were fixed on ERISM substrates and immunostained for the focal adhesion protein vinculin, the cytoskeleton protein actin and the mechanosensitive transcription co-activator TAZ (Figure 2A) These data show that the knockdown of Willin/FRMD6 leads to general suppression of the mechanical activity of SH-SY5Y cells on various critical levels as well as to morphological changes towards smaller but more elongated cells that form an increased number of small cell extensions significantly increased in cells with higher Willin/FRMD6 expression These findings suggest that the reduction of Willin/FRMD6 protein levels in SH-SY5Y cells leads to the activation of known signaling transduction pathways and promotes neuronal differentiation This shows that knockdown of Willin/FRMD6 phenocopies the mechanical activity of RA-induced neuronal differentiation the observed changes were attributed to the function of Willin/FRMD6 as an upstream activator of the Hippo signaling pathway Willin/FRMD6 may have a similar role in focal adhesion complexes of SH-SY5Y cells We also found that an increase of Willin/FRMD6 concentration the changes in phenotype observed for knock-down cells were more pronounced than the reversed effect seen in overexpressing cells our data show that Willin/FRMD6 knockdown not only promotes neuronal differentiation of SH-SY5Y cells but also results in increased proliferation opens the door for future studies on how Willin/FRMD6 may not only influence neuronal differentiation but may also play a role in neurodegeneration All data needed to evaluate the conclusions in this article are presented in the article and/or Supplementary Materials. The research data supporting this publication can be accessed at: https://doi.org/10.17630/e0983924-b0fc-41ac-9c05-35758150df00 All authors contributed to the project and experimental design DC and WY conducted experiments and analyzed data NK and AT-L wrote the first draft of the manuscript with contributions by MG and FG-M All authors contributed to the article and approved the submitted version This research was financially supported by an Engineering and Physical Sciences Research Council (EPSRC) Programme Grant (EP/P030017/1) and by the Biotechnology and Biological Sciences Research Council (BBSRC) Tools and Resources Development Fund (BB/P027148/1) The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fncel.2020.552213/full#supplementary-material The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest FAT1 cadherin acts upstream of Hippo signalling through TAZ to regulate neuronal differentiation Sprouty4 is an endogenous negative modulator of TrkA signaling and neuronal differentiation induced by NGF Willin/FRMD6 expression activates the Hippo signalling pathway kinases in mammals and antagonizes oncogenic YAP Store-operated Ca2+ entry in proliferating and retinoic acid-differentiated N- and S-type neuroblastoma cells Comparative profiling of cortical gene expression in Alzheimer’s disease patients and mouse models demonstrates a link between amyloidosis and neuroinflammation The FERM domain: a unique module involved in the linkage of cytoplasmic proteins to the membrane Sequential treatment of SH-SY5Y cells with retinoic acid and brain-derived neurotrophic factor gives rise to fully differentiated Identification of a novel inhibitor of mitogen-activated protein kinase kinase Optimal myelin elongation relies on YAP activation by axonal growth and inhibition by Crb3/Hippo pathway Progress toward understanding the molecular mechanisms of neurotrophic factor signalling PubMed Abstract | CrossRef Full Text | Google Scholar A novel 4.1 ezrin radixin moesin (FERM)-containing protein ERK-mediated mechanochemical waves direct collective cell polarization Genome-wide and gene-based association implicates FRMD6 in Alzheimer disease Willin and Par3 cooperatively regulate epithelial apical constriction through aPKC-mediated ROCK phosphorylation “Considerations for the use of SH-SY5Y neuroblastoma cells in neurobiology,” in Neuronal Cell Culture Methods in Molecular Biology (Methods and Protocols) Google Scholar Long-term imaging of cellular forces with high precision by elastic resonator interference stress microscopy López-Carballo Activation of the phosphatidylinositol 3-kinase/Akt signaling pathway by retinoic acid is required for neural differentiation of SH-SY5Y human neuroblastoma cells KIBRA exhibits MST-independent functional regulation of the Hippo signaling pathway in mammals an upstream component of the Hippo signaling pathway orchestrates mammalian peripheral nerve fibroblasts Whole brain surface-based analysis identified brain atrophy associated with SNPs in frmd6 linked to Alzheimer’s disease Glucose induced MAPK signalling influences NeuroD1-mediated activation and nuclear localization Phospholipase C-η2 is required for retinoic acid-stimulated neurite growth Regulation of Hippo signaling by EGFR-MAPK signaling through Ajuba family proteins Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling The neural cell adhesion molecule NCAM regulates neuritogenesis by multiple mechanisms of interaction Genetic analysis of quantitative phenotypes in AD and MCI: imaging Tilston-Lünel Crumbs 3b promotes tight junctions in an ezrin-dependent manner in mammalian cells YAP/TAZ upstream signals and downstream responses The TrkB-T1 receptor mediates BDNF-induced migration of aged cardiac microvascular endothelial cells by recruiting Willin Negative regulation of Yap during neuronal differentiation YAP-dependent induction of amphiregulin identifies a non-cell-autonomous component of the Hippo pathway Gather MC and Gunn-Moore FJ (2020) Willin/FRMD6 Influences Mechanical Phenotype and Neuronal Differentiation in Mammalian Cells by Regulating ERK1/2 Activity Received: 15 April 2020; Accepted: 17 August 2020; Published: 04 September 2020 Copyright © 2020 Kronenberg, Tilston-Lunel, Thompson, Chen, Yu, Dholakia, Gather and Gunn-Moore. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) distribution or reproduction in other forums is permitted provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited in accordance with accepted academic practice distribution or reproduction is permitted which does not comply with these terms *Correspondence: Malte C. Gather, bWNnNkBzdC1hbmRyZXdzLmFjLnVr; Frank J. Gunn-Moore, ZmpnMUBzdC1hbmRyZXdzLmFjLnVr † These authors have contributed equally to this work Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher 94% of researchers rate our articles as excellent or goodLearn more about the work of our research integrity team to safeguard the quality of each article we publish Emmanuel Macron’s business-friendly liberalism could be enough to spare France from the National Front it’s no safe bet against the populist far right French economic minister Emmanuel Macron got into a heated argument with protesters Opposed to his government’s unpopular labor reforms they’d just disrupted what was meant to be a stress-free photo op at a tech school in the sleepy Southern town of Lunel As the minister smiled and shook hands inside the sound of the megaphone blaring from across the street was impossible to ignore: “Mr He had some more pointed advice for a younger protester an unemployed twenty-one-year-old who quipped that he didn’t have the cash to pay for a nice suit like the one Macron was sporting “You’re not gonna scare me with your T-shirt,” the minister retorted “The best way to pay for a suit is to work for one.” Since officially launching his presidential campaign last November Macron has avoided slip-ups of this nature a near-perfect distillation of his political vision: detached from the struggles of ordinary people spiteful of unions and left-wing social movements and enamored with the liberating power of free enterprise While current polls show him handily defeating the odious Marine Le Pen of the far-right National Front (FN) in the second round of France’s presidential election—and there’s no question he’s a far preferable option in this case—the former investment banker-turned-minister is a dangerous politician in his own right the success of the thirty-nine-year-old founder and leader of the independent En Marche How can a candidate associated with such unpopular ideas—a backer of finance For one, thanks to some top-notch branding and messaging. From the very beginning, Macron has posed as a renegade candidate, promising to take on “the system” and shake it up. “From the inside, I saw the emptiness of the system,” he thundered in his opening campaign speech last November he claimed to be “the only outsider” in the race However you choose to define “the system”—ultra-tight political cliques in charge of parties and governments cultural elites with friends in high places or brute economic powers fueling inequality—Macron embodies it A native of Amiens in northern France, Macron attended the prestigious Henri IV prep school in Paris. From there, he moved on to Sciences Po, a highly selective university that specializes in politics and international relations, before graduating from the ultra-elite École Nationale d’Administration, an institution that literally produces France’s ruling class Designed to groom high functionaries and ministers the school counts as alumni three of the Fifth Republic’s seven presidents including the current resident of the Élysée Palace Macron joined the ranks of the Inspection générale des finances an interdepartmental financial auditing corps was named adjunct general reporter of the newly launched Commission for the Liberation of French Growth Created by President Nicolas Sarkozy and chaired by Jacques Attali once a top adviser to François Mitterrand’s Socialist government the body was a French analog to the Simpsons-Bowles Commission: under the guise of nonpartisan economic reform its recommendations boiled down to an assault on the social safety net Its conclusions have inspired many a “reformist” since from mainstream conservatives to Macron himself It all but confirmed his personal political ambitions In August Hollande’s former protégé resigned his post to focus on preparing his own presidential campaign Macron’s record in government was unimpressive: the Socialists failed to tackle unemployment even as they implemented reforms that doomed the party’s electoral chances The fact that a graduate of one of the country’s grandes écoles who made millions in the Rothschild banking empire and set economic policy for the sitting government would even attempt to portray himself as “anti-system” says a lot about our current political moment from a real-estate mogul successfully posing as an anti-establishment champion of the working class Thanks to Hollande’s dismal presidency working-class and other traditionally left-wing voters are turning their backs on the PS The party’s presidential nominee Benoît Hamon has struggled to reverse the trend Some of these electors now opt for independent left-wing candidate Jean-Luc Mélenchon But as recent European and regional races showed many low-income voters prefer abstention and the National Front the standard-bearer of the mainstream right Les Républicains Aside from an especially cruel and unpopular platform the former prime minister has been battered by a series of scandals over the alleged diversion of public funds Party leaders and the conservative base alike grow increasingly critical Those who do plan to cast ballots are turning away from the parties that have dominated French political life for a generation Neither of the leading candidates belong to the two major parties Le Pen has for years swelled the ranks of her far-right base with disillusioned working-class people has managed to attract a more socioeconomically diverse swath of voters fed up with politics as usual released in conjunction with his formal entry into the race sheds some light on his no-labels identity Macron describes himself as “progressive.” The word isn’t too familiar to French ears: Left-leaning voters think of something closer to its meaning in the American context it sounds vaguely pro-business and pro-innovation “Our political life is organized today around an old cleavage that no longer allows us to respond to the challenges of the world and our country,” Macron writes in Révolution “Today the great questions of our time are our relationship to work profoundly shaken up by environmental and technological questions our relationship to the world and to Europe the protection of individual liberties and of an open society in a world filled with risks the left and right are profoundly divided and as such They haven’t updated their ways of thinking to the reality that now surrounds us.” to translate this sort of blueprint into actual policy prescription is to risk collapsing the entire project No matter how one describes one’s guiding philosophy campaign proposals interact in a real world that remains preoccupied with the question of how resources are distributed and who benefits from what; in short Macron didn’t actually release his platform until early March—that is less than eight weeks before the first round of the election With the curtain finally lifted, the regressive nature of his candidacy can no longer be denied. One of the most worrying elements is a plan to further overhaul collective bargaining in favor of big business. Just 8 percent of French workers belong to unions, but by law, the contracts they negotiate cover nearly the entire labor force from national and industry-wide agreements to deals with individual companies Last year’s reforms undercut large-scale labor agreements by allowing company-level negotiations to take priority under certain circumstances authorizing companies to bargain over issues currently codified by law such as hours and the general organization of work Since the terrorist attacks of November 2015 the law authorizes police to seize data from phones and computers and conduct searches without the approval of judges In this year’s strange and unpredictable campaign Macron’s mealy-mouthed liberalism could be enough to spare France from the National Front it’s not exactly a safe bet against the mounting threat of the populist far right European politics increasingly take the shape of a bleak contest then at least in rhetoric: on the one hand a strain of xenophobic populism anchored to the national welfare state; on the other a sort of cosmopolitan liberalism in defense of free trade and enterprise This is what the Brexit debate looked like German and Dutch politicians increasingly talk along these lines And it’s essentially what defines the conflict between Macron and Le Pen Squeezed out of the discussion is the left: a force capable of defending public services while distinguishing between the free flow of people and capital Macron’s plans to cut state spending and reshape collective bargaining will likely hurt wage-earners and fuel inequality Le Pen’s “France First” program would be far worse unleashing an unprecedented wave of racism and xenophobia The first scenario is still much easier to imagine Pro-EU liberals cannot continue to ignore the plight of working people and kowtow to business while assuming politics will go on as usual events on the other side of the Atlantic should have been And yet Macron’s bargain remains essentially unchanged however “progressive” he considers it Cole Stangler is a journalist living in Paris A former staffer at In These Times and International Business Times his work has also appeared in the Village Voice Marine Le Pen’s National Rally is reaping the fruits of a long history of anti-European sentiment As Emmanuel Macron bypasses French democracy to enact a sweeping pro-business agenda French politics has become less predictable than at any time since the founding of the Fifth Republic It remains to be seen whether this volatility will reward the left—or the populist far right Please consider donating to Dissent. Your contribution will ensure that we continue to publish articles like this one. Donate $10, $50, or $500; we are grateful for gifts of all sizes The film tells the story of 28 year old Haska who after losing her husband at the beginning of the war decides to leave besieged Sarajevo through the mountains with her 9 year old son Denis It was shot in 21 days during February and March 2016 in Sarajevo and Čelebići It is currently in post-production and is expected to premiere in 2017 who spent the war in Bosnia and Herzegovina living with different families had an opportunity to witness the difficulties women were facing during the war and decided to concentrate on female characters but never regular women taking care of their house Lunel said about the films shot about the war in Bosnia and Herzegovina so far Click Here for Statements from European Film Organisations The Film New Europe (FNE) Association is the networking platform for film professionals in the CEE/SEE/Baltics region The webportal and FNE newswire was chosen as the MAIN TOOL to achieve the network’s objectives of the sharing of know how visibility of regional cultural diversity and finally the VOICE of the region FNE’s objectives include VISIBILITY for the region and AUDIENCES for films by providing a special focus on the region Inside Operation Tupac: Pakistan’s secret project to burn Kashmir the Zia-style general shaping Pakistan’s faith-driven military revival not preachers': India's strong message for EU amid LoC tensions (Catch all the Business News, Breaking News, Budget 2024 Events and Latest News Updates on The Economic Times.) Subscribe to The Economic Times Prime and read the ET ePaper online Choose your reason below and click on the Report button This will alert our moderators to take action Your Reason has been Reported to the admin It seems like you're already an ETPrime member with Login using your ET Prime credentials to enjoy all member benefits Log out of your current logged-in account and log in again using your ET Prime credentials to enjoy all member benefits LANSING - Phil and Lunel Haysmer didn't know for sure until a few days ago that lava from Kilauea volcano had destroyed their home in Hawaii From a room in a friend of a friend's house 15 miles from Hilo watched video footage taken by a drone flying over their property.  Neighbors who had been back to their subdivision in the Leilani Estates told the Haysmers they were "pretty sure" the couple's 3,500-square-foot home was gone — but they hadn't been sure Nineteen lava outbreaks in the neighborhood prevented them from getting a closer look.   But the drone's video gave the Haysmers a definitive and devastating look at the ruins of their home Pieces of their home's corrugated metal roof blackened with soot and warped by heat were strewn around the one-acre property tropical plants and pineapple patch Lunel Haysmer planted when they bought the property six years ago were gone too.  More: Before and after: Satellite images show devastation of the Leilani Estates neighborhood from Hawaii volcano but the heat that came with it started a fire that destroyed everything they owned Michigan State University graduate and former Lansing Community College professor The loss of their home has been an emotional blow but they have no intention of leaving Hawaii "The initial event basically happened in our back yard," he said Tuesday who retired and moved to Hawaii six years ago, were evacuated from their neighborhood May 3 minutes before the first of 19 lava outbreaks happened less than a half mile from their house It had been the second family emergency that day had been taken by ambulance to a hospital after suffering a seizure both artists who ran a gallery for more than two decades in California, had been listening to the sounds of pressure building underground the entire day At times it sounded like they were "standing right right next to a jet engine," he said When the evacuation order was issued Phil Haysmer said he was given 15 minutes to leave the dogs (the couple has four) and their bowls," he said Horrocks: 'There's simply nothing else like it' Meridian Magnesium fire report to be done Thursday Dan Carr remembered as 'compassionate' teacher The Haysmers are among nearly 2,000 people who have been barred from their homes for nearly two weeks, while lava pours through the neighborhood. At least 36 structures, including 26 homes, have been destroyed, according to news reports Phil Haysmer said they're getting by thanks to the generosity of strangers and friends alike who are housing them and helping them while they decide how to move forward along with the couple's pick-up truck and everything in Phil Haysmer's lower-level art studio waiting to see what insurance will cover," Phil Haysmer said Insurance staff haven't been able to reach the couple's property yet but they're grateful to everyone who's reached out to help them, Lunel Haysmer said Friends in California started an online fundraising page to collect donations for the couple and Phil Haysmer said some of those contributions have come from Michigan friends and family owned Haysmer's Barber Shop in downtown Charlotte for nearly 50 years "It's hard to be placed in a place where all you can do is receive," Lunel Haysmer said "But it's an opportunity to look at things have a different point of view." She's chronicling it on her blog at www.haysmerhawaii.blogspot.com  Phil Haysmer said Hawaii's "aloha spirit" is carrying them through the ordeal 'We will help you and someday you may need to help us,'" he said It is a cultural community spirit of help that's an amazing thing to be a part of." Contact Rachel Greco at (517) 528-2075 or rgreco@lsj.com. Follow her on Twitter @GrecoatLSJ Essential digital access to quality FT journalism on any device Complete digital access to quality FT journalism with expert analysis from industry leaders Complete digital access to quality analysis and expert insights complemented with our award-winning Weekend Print edition Terms & Conditions apply Discover all the plans currently available in your country See why over a million readers pay to read the Financial Times Ecological Social Governance (ESG) and effective loyalty management are vital drivers of progress for restaurants the Digital Talk "From Rewards to Responsibility: Loyalty and ESG in Restaurants" presents an exclusive opportunity to gain valuable insights from two major European restaurant companies – Big Mamma and Sticks ‘n’ Sushi – as they discuss their experiences Register now for our free digital talk on August 30th, 2023! 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Send an email to the editors Here you can purchase the rights of use for this article. Print article Suggestions & comments to info@food-service.de Colin RandallMay 31