Laura Garcia Italian food holds a very special place in my heart who doesn’t love freshly made pasta or a perfectly crunchy pizza The flavors are a combination of rich and hearty that’s truly impossible to resist I make it my mission to find the best Italian restaurant there is– and Chicago was no exception has not only made me love Italian food even more From my first visit when I first arrived at Northwestern to when I hosted my birthday dinner here in July Ciccio Mio has quickly become my favorite restaurant in all of Chicago it feels like you’re stepping into a different world one that combines the 1920s atmosphere with contemporary flair vibrant wallpaper with numerous paintings adorning the walls and plush velvet chairs all come together to create an intimate savoring not just your meal but the entirety of the dining experience What I love most about the restaurant’s ambiance is how perfectly it complements the food– luxurious Ciccio Mio beautifully blends traditional Italian flavors with an inventive twist ensuring every dish feels both familiar and exciting While their one-page menu might make you feel like options are limited Though not quite the traditional Italian dish (perhaps more American) the mozzarella sticks are still worth a try Their crispy exterior adds texture to the melted mozzarella that’s mixed with the rich The sweetness and freshness of the tomatoes in the marinara sauce pair beautifully with the sticks.  but here they add a refreshing and pleasant aftertaste to the focaccia The crunchiness of the bread is complemented by caramelized onions and crispy delicate richness to the bread with a touch of acidity from the lemon juice squeezed on top I cannot even begin to explain how delicious these potatoes are The amount of flavor they carry is amazing and each bite tastes like heaven They’re crispy on the outside and soft on the inside What elevates them are the chicken jus and garlic confit Of all the pasta options Ciccio Mio offers The sauce is rich and velvety – a delicious blend of creamy tomato with the earthy tang of vodka Thanks to the rigatoni being perfectly al dente This rigatoni is the perfect example of “simple is best,” offering a depth of flavor that leaves you craving more No better way to end your hearty stay at Ciccio Mio than with this delicious dessert The combination of the nutty almond flavor with salt flakes and the smooth consistency of the gelato is pure bliss just enough to satisfy one’s sweet tooth.  The dining experience at Ciccio Mio is all the more remarkable for the fact that it’s been awarded a Michelin star a testament to the effort that went into perfecting each dish the prices remain incredibly reasonable for such a renowned restaurant You can indulge in delicious food without feeling like you’re breaking the bank.  Located a few blocks away from the Riverwalk Ciccio Mio offers the ideal starting point for a post-dinner stroll where you can take in the sights of downtown Chicago Ciccio Mio isn’t just a restaurant; it’s a celebration of flavors dear to my heart Each visit is a joyful reunion with the cuisine I love and every dish blends tradition and innovation Whether for a special occasion or a well-deserved night out Ciccio Mio promises an unforgettable experience that your taste buds will thank you for To learn more about Ciccio Mio, click here To make a reservation, click here.  Contact Us Editorial Staff NBN Archive (2019 – 2024) Proudly Powered by WordPress | Theme Rumblepress by WebsiteinWP | Use of this site constitutes acceptance of our User Agreement Chenega has purchased SecuriGence and will integrate the veteran-owned small business into its military intelligence and operations strategic business unit The Alaska Native corporation said Wednesday the integration planning has begun and the acquisition will expand Chenega MIOS’ portfolio with the addition of systems engineering software engineering and cloud and virtual management capabilities in support of Department of Defense and Intelligence Community customers Chenega MIOS President John Campagna described the addition of SecuriGence as a “game-changer.” Virginia-based SecuriGence is a minority-owned business focused on delivering information technology operations software development and systems engineering services to the government sector “SecuriGence brings well over a decade of exceptional service and support to the Defense and Intel communities Their core competencies are a ‘wish list’ of abilities that would normally be found in a Fortune 500 engineering house Their leadership is well respected and admired throughout the DoD/Intel community,” Campagna added Established in 2010, Lorton, Virginia-headquartered Chenega MIOS is a shared services division of Chenega Corporation and provides advanced analytics data visualization and other IT services for federal defense and commercial customers worldwide The premier source of breaking business news for the government contracting industry to-the-point stories of the most significant contract awards M&A activities and financial results of the sector’s most notable players GovCon Wire is always on top of the most recent contracting sector activity and is updated in real time as the news breaks Important URLs: About us – Government Contracting FAQ – Guest Contributions – 2024 Events and just under 4 blocks a game this past season as she led Mio to a district title McGregor already has the school points record at Mio with over 1550 points already as just a sophomore As a freshman she set state scoring records averaging over 41 points per game and she talked about what it meant to her to be named the back-to-back player of the year in division 4 It’s super cool to bring that to our community and everything.” Said McGregor “It’s awesome to bring that back because I think it was four years ago or three years ago so it’s great to have like some honor and recognition come to our school.” The AAU season has already started for McGregor and she’s hoping to lead the thunderbolts on a deeper run in 2026 You are using an outdated browser. Upgrade your browser today or install Google Chrome Frame to better experience this site that we announce the sudden passing of our wife affectionately known to her friends and family as “Debby.” Loving wife of Oliviero to whom she was hopelessly devoted forever Debby’s caring and loving ways were magnetic Her optimism and bubbly cheer complimented her fierce dedication to her friends and family Daughter to Ronald(1982) and Thelma(2008) Ettinger Daughter-in- Law to Giovanni(1988) and Adelia Mio(2007) sister-in-law to Tiziana and Raymond(2007) A proud West sider growing up on Barrymore Lane Debby attended JL Forster Secondary School she met her soulmate and began their lifelong journey with her best friend Debby successfully graduated from nursing school turning her natural gift for caring for others into a fulfilling career as a Registered Nurse at IODE Met and Detroit Receiving. Debby was inspired to pursue her dreams with the support of her cherished cugina they became role models for Jessica and Ava to follow their own paths into nursing She always had a beer ready for Ollie at the end of a race and never missed cheering on her sons and her Nini The Caribbean and the South Pacific were highlights of their adventures together where they collected more stories and friends than they could ever hope to count or retell… though they do try.  She was loved by those near and far She will be greatly missed by family in Italy and lifelong friends she made in Hawaii whether it was having lunch with the ladies doing amazing work with the Sunshine Girls She always looked forward to getting together Debby was happiest sharing her love for animals: koala bears To know Debby was to know her unconditional love It is impossible to sum her up in a few paragraphs She was happiest when surrounded by family and friends with a margarita in her hand and smile on her face Visitation will be held at Windsor Chapel Funeral Home – South Chapel 3048 Dougall Avenue (519-253-7236) on Saturday from 11:00am until time of prayer service at 12:00 noon in the chapel A private family interment at Heavenly Rest Catholic Cemetery will take place at a later date As an expression of sympathy and greatly appreciated by the family, memorial donations may be made to the The Windsor/Essex County Humane Society , The Kidney Foundation of Canada , or consider signing up to be a donor at Trillium Gift of Life Network  Get Directions Central Chapel | (519) 253-72341700 Tecumseh Rd Banwell Chapel | (519) 253-723511677 Tecumseh Rd South Chapel | (519) 253-72363048 Dougall Avenue, Windsor, Ontario N9E 1S4     Proudly Canadian | Owned & Operated by Arbor Memorial Inc. Armstrong Williams takes on the news of the week and asks the questions you want answered. Don’t miss our weekly town hall. Trespassing suspect arrested after altercation with deputy at Mio's Family Dollarby UpNorthLive Newsroom (WPBN/WGTU) - - One person is in jail after a trespassing incident led to an altercation with a deputy in Oscoda County Deputies were called to Family Dollar in Mio last Sunday afternoon For a disturbance involving someone who had been on the property for an extended period of time and they left for about an hour but were called back to the store for another disturbance The sheriff's office says the person became agitated and was placed under arrest for trespassing The sheriff's office says the person tried to take a deputy's firearm and taser This led to the deputy using an electronic control device to gain compliance The person was then taken into custody and assessed for minor injuries One deputy sustained an injury during the incident that is still being evaluated The incident is under review and no further details are available at this time the leading provider of chat interoperability solutions today announced a strategic partnership with 66degrees a premier Google Cloud Premier Partner specializing in data cloud and productivity and collaboration solutions This collaboration is set to enhance Mio's already robust cross-platform capabilities for users of Google Workspace 66degrees brings a wealth of experience and a proven track record in helping organizations leverage Google Cloud’s capabilities to accelerate their digital transformation This partnership will enable Mio to provide a more seamless communication experience for enterprises who rely on Google and other platforms Visit www.m.io to learn more about how Mio's partnership with 66degrees is unifying workplace communication for modern enterprises Mio powers effective communication across multiple messaging platforms by enabling users to collaborate seamlessly. Founded in 2016 in Austin, Texas by Tom Hadfield and James Cundle, Mio has secured $17 million in funding from major investors such as Zoom, Cisco, and Y Combinator. For more information, please visit www.m.io Metrics details Reproductive success relies on proper establishment and maintenance of biological sex the primary gonad is initially ovary biased We previously showed the RNA binding protein (RNAbp) we identified Rbpms2 targets in oocytes (Rbpms2-bound oocyte RNAs; rboRNAs) We identify Rbpms2 as a translational regulator of rboRNAs which include testis factors and ribosome biogenesis factors genetic analyses indicate that Rbpms2 promotes nucleolar amplification via the mTorc1 signaling pathway specifically through the mTorc1-activating Gap activity towards Rags 2 (Gator2) component our findings indicate that early gonocytes are in a dual poised bipotential state in which Rbpms2 acts as a binary fate-switch Rbpms2 represses testis factors and promotes oocyte factors to promote oocyte progression through an essential Gator2-mediated checkpoint thereby integrating regulation of sexual differentiation factors and nutritional availability pathways in zebrafish oogenesis we envisioned two non-mutually exclusive hypotheses for Rbpms2 functions in ovary specific differentiation of germ cells (1) Rbpms2 actively represses translation of testis factors that are expressed in the early germline cells of ovaries and (2) promotes translation of factors that allow for oocyte growth and differentiation upstream of an unknown oocyte progression checkpoint and ribosome biogenesis in the context of oocyte development remains to be fully understood we identified an enrichment of rboRNAs in early oocytes the stages crucial for further oocyte and ovary differentiation in zebrafish Subsequent RNA-seq analysis implicates Rbpms2 as a rboRNA translational regulator likely acting to repress testis factors and activate ovary factors RNA-seq and immunohistological analyses indicate that Rbpms2 promotes nucleolar assembly we hypothesized that early gonocytes are in a dual poised bipotential state where Rbpms2 dynamically regulates oocyte and testis rboRNAs and acts as a switch upstream of a nutrient-responsive pathway positively impacting the translational capacity of oocytes genetic analyses place Rbpms2 upstream of the mTorc1 pathway-activating protein disrupts nucleolar composition and compromises ovary maintenance Genetically manipulating regulators of the metabolic stress sensing arm of mTorc1 in mios mutants (mios−/−) did not consistently prevent oocyte loss while active forms of mTOR restored oogenesis and female sex determination in mios−/− fish we have identified an RNA binding protein (RNAbp)-mediated binary fate switch that activates a Gator2-mediated checkpoint essential for oocyte development thereby integrating sexual differentiation factors and nutritional availability pathways during zebrafish oogenesis and female sex determination we sought to determine the germline-specific RNA targets of Rbpms2 which are expected to be critical for successful oogenesis and reproductive functions Number of RNAs in a category are given in the header and rboRNAs associated with testis fates are specified in the header as well c Top 10 GO biological processes associated with rboRNAs Dark blue indicates not significant and increasing lighter shades of blue indicate increasing significant values d Volcano plot of differential gene expression in 21 dpf bipotential wildtype and rbpms2 DM gonads from bulk RNA sequencing rboRNAs are overlayed in blue and all RNAs are labeled in gray (c-d) Differential Gene Enrichment was calculated with the DESeq2 package adjusted P = 0.05 adjusted P (−log10) = 1.30 and fold change of 2.00 e Representative gene set enrichment plots for GO Ribosome Biogenesis and GO Regulation of TOR Signaling from bulk RNA sequencing of 21 dpf wildtype versus rbpms2 DM gonads Dark red indicates more frequently detected in rbpms2 DM gonads pink indicates slightly more expressed in rbpms2 DM gonads light blue indicates slightly more expressed in wildtype gonads and dark blue indicates more frequently expressed in wildtype gonads and FDR were calculated via the Gene Set Enrichment Analysis algorithm Source data are provided as a Source Data file we hypothesize that Rbpms2 negatively regulates several rbtRNAs in gonocytes to promote the early stages of oogenesis and female sex differentiation This suggests that Rbpms2 may positively regulate rboRNAs related to ribosome biogenesis and ribonucleoprotein biogenesis and assembly to ensure sufficient levels of each are present for oogenesis This suggests that Rbpms2 does not regulate RNA abundance but could instead function at the level of translational control These findings are consistent with the following hypotheses: (1) Rbpms2 likely functions to repress translation of rbtRNAs expressed in early gonocytes in ovaries and (2) promote translation of oocyte factors including ribosome and ribonucleoprotein complex biogenesis regulators to support growth though alternative mechanisms cannot be excluded These results suggest that early gonocytes exist in a bipotential state wherein Rbpms2 mediates a binary fate-switch likely by indirectly or directly repressing rbtRNA translation and promoting nucleolar formation and oocyte development via mTor signaling to ensure sufficient ribosome biogenesis for oogenesis a Schematic of nucleolar development from mitosis to prophase I arrest the singular nucleolus of the germ cell disassembles new nucleoli are amplified through seeding by rDNA loci and nucleoli begin to expand and ribosome biogenesis continues up until meiotic arrest at diplotene c Transmission electron micrographs of 35 dpf wildtype and rbpms2 DM oocytes Dashed lines outline oocyte cytoplasmic membrane and black arrowheads indicate nucleoli d Quantification of number of nucleoli per oocyte in wildtype (n = 2 fish) and rbpms2 DM (n = 2 fish) gonads A minimum of 20 prophase I cells per genotype were analyzed and categorized into the following groups: 1 nucleolus (blue) f p-Ubtf (active RNA pol I; purple) and RNA pol II (yellow) immunostaining in 31-35 dpf wildtype (rbpms2aae30; rbpms2bsa9329 heterozygous mutant; n = 4) and rbpms2 DM (rbpms2aae30; rbpms2bsa9329 double homozygous mutant; n = 4) germ cells Enlarged views of boxed regions show representative localization of p-Ubtf in wildtype and rbpms2 DM oocytes and white arrows indicate p-Ubtf localization in nucleoli h Fibrillarin (yellow) immunostaining in 29-33 dpf wildtype (rbpms2aae30; rbpms2bsa9329 heterozygous mutant; n = 4) and rbpms2 DM (rbpms2aae30; rbpms2bsa9329 double homozygous mutant; n = 4) germ cells Enlarged views of boxed regions show representative localization of Fibrillarin in wildtype and rbpms2 DM oocytes e–h Germ cells are labeled by Ddx4 (teal) and nuclei are labeled with DAPI (white) We also observed localization of RNA pol I to the cytoplasm of oocytes and early meiotic cells This finding indicates that recruitment of RNA pol I is compromised and suggests that rDNA nucleation and/or nucleolar seeding by rRNA transcription may be disrupted in rbpms2 DM oocytes nucleolar localization of RNA pol I and Fibrillarin were consistent with ultrastructural data indicating few nucleoli per oocyte in rbpms2 DM ovaries these results indicate that nucleolar recruitment of ribosome biogenesis factors is dysregulated in rbpms2 DM oocytes and that Rbpms2 contributes to ribosome biogenesis and may do so by regulating nucleation and/or seeding by regulating translation of ribosome biogenesis factors that promote nucleolar development in primary oocytes Transcript IDs evaluated for each component are listed and purple boxes indicate the protein component or complex of the pathway a given factor belongs to g–l Immunostaining for Mios (red) in 29-31 dpf wildtype (rbpms2aae30; rbpms2bsa9329 heterozygous mutant n = 4) and rbpms2 DM (rbpms2aae30; rbpms2bsa9329 double homozygous mutant; n = 4) germ cells Germ cells are labeled with Ddx4 (teal) and nuclei are labeled with DAPI (white) h–l Dashed outline indicates oocytes and shows Mios and DAPI localization in indicated cells m Quantification of the intensity of Mios normalized to the intensity of Ddx4 for wildtype (rbpms2aae30; rbpms2bsa9329 heterozygous mutant; n = 11 cells from 3 individual fish) and rbpms2 DM (rbpms2aae30; rbpms2bsa9329 double homozygous mutant; n = 11 cells from 3 individual fish) oocytes Two-tailed paired equal variance student’s t tests were performed for the indicated groups boxes indicate upper (75th) and lower quartiles (25th) whiskers indicate data within 1.5 times the inner quartile range and error bars represent minimum and maximum values Any data outside of this range are plotted as individual points these results suggest that Rbpms2 is a positive regulator of mios RNA translation and therefore acts upstream of mTorc1 signaling a Schematic of the Mios protein and mutagenized regions (black arrowheads) in the Miosms20 and Miossa proteins Truncated proteins are shown below with highlighted DNA mutations and corresponding genotyping assays The * in Miossa indicates the induced stop codon Developing sperm are outlined with dashed lines and scale bar is 50 µM Pink represents female fish and blue indicates male fish Number of fish screened are indicated for each group Statistical significance was evaluated by performing a two-tailed χ2 test with Bonferroni correction against the +/+ control group g Fibrillarin (yellow) immunostaining in 35 dpf wildtype (n = 7) and mios-/- (n = 10) germ cells Enlarged views of boxed regions show representative localization of Fibrillarin in wildtype and mios-/- oocytes h Quantification of the Fibrillarin puncta area normalized to oocyte nuclear area for wildtype (n = 9 cells from 3 individual fish) and mios-/- (n = 9 cells from 3 individual fish) oocytes Two-tailed paired equal variance Student’s t tests were performed for the indicated groups j p-Ubtf (active RNA pol I; purple) and RNA pol II (yellow) immunostaining in 35 dpf wildtype (n = 4) and mios-/- (n = 4) germ cells Enlarged views of boxed regions show representative localization of p-Ubtf (red) and RNA pol II (white) in wildtype and mios-/- oocytes j Germ cells are labeled by Ddx4 (teal) and nuclei are labeled with DAPI (white) This observation together with Fibrillarin staining indicates the nucleoli of mios-/- may be immature or have impaired recruitment of certain ribosome biogenesis factors This suggests that overt nucleolar architecture is not disrupted; however or retention of specific factors may be impaired in the absence of Mios we conclude that impaired ovary differentiation is due to mutation of mios and that Mios is required for oocyte differentiation in zebrafish a–d Immunostaining of p-Ps6k (purple) in 35 dpf wildtype (n = 7) and mios-/- (n = 10) germ cells b Arrows indicate p-Ps6k localization in mitotic and early meiotic nuclei Adjacent panel shows p-Ps6k and DAPI localization in indicated cells e Quantification of the intensity of p-Ps6k normalized to the intensity of Ddx4 for wildtype (n = 21 cells from 7 individual fish) and mios-/- (n = 25 cells from 8 individual fish) f mTORca transgene: the human mTORca expression is driven by the germline ziwi promoter and the heart specific promoter cmlc2 drives mCherry expression as a selectable marker Scheme depicts injection into 1-cell embryos and generation of stable Tg[ziwi:mTORca] lines g Sex ratios for 90 dpf+ fish with ms49 and ms64 mTORca alleles Pink represents female and blue represents males; numbers of fish screened are indicated for each group Non-transgenic siblings are indicated by - and transgenic siblings are indicated with + and the specific mTORca alleles are indicated Statistical significance was evaluated by performing a two-tailed χ2 test with Bonferroni correction against the mios +/+; +/- non-transgenic control group h Fertility assays and quantifications of total eggs and fertility rates for ms49 and ms64 Tg[ziwi:mTORca] fish (n = 3 fish per genotype and transgenic condition) All other comparisons were non-significant These findings indicate incomplete suppression of oocyte loss or that Mios may have mTorc1 independent roles These results demonstrate that the requirement for Mios in oogenesis is conserved in ovaries Mios acts through mTorc1 signaling and mTorc1 activation is sufficient for successful oogenesis in the absence of Mios a Sex ratio graph for 60 dpf+ mios; spo11uc73 double heterozygous Statistical significance was evaluated by performing a two-tailed χ2 test with Bonferroni correction against the mios+/-; spo11+/uv73 control group white) in mios-/- (mios;spo11 mutant wildtype/mutant heterozygous fish n = 6) and (c) mios-/-; spo11uc73/uc73 double mutant fish (n = 7) Developing oocytes (b) and sperm (c) are outlined with dashed lines a Sex ratio graph for 60 dpf+ mios; tsc2vu242 double heterozygous Statistical significance was evaluated by performing a two-tailed χ2 test with Bonferroni correction against the mios+/-; tsc2+vu242 control group b Rhebca transgene: the rat Rhebca expression is driven by the germline ziwi promoter and the heart specific promoter cmlc2 drives mCherry expression as a selectable marker Sex ratios are presented for 60 dpf+ fish with the ms44 Pink represents female and blue represents males; number of fish screened are indicated for each group Statistical significance was evaluated by performing the two-tailed χ2 test with Bonferroni correction against the +/- transgenic control group Rbpms2 functions to repress translation of rboRNAs related to testis fates and promotes translation of rboRNAs related to mechanisms supporting oogenesis like ribosomal factors Rbpms2 functions upstream of nucleoli amplification and nucleolar localization of RNA pol I Gator2 and Mios activity increase mTorc1 signaling and the nucleoli of differentiating oocytes expand and mature to support high levels of rRNA synthesis they develop an additional compartment that supports pre-ribosome biogenesis If sufficient ribosomes are present the cells continue through oogenesis If a sufficient ribosome pool is not attained then the gonocytes abort oogenesis and switch to spermatogenesis We anticipate this pathway operates in oocytes independent of sex determination systems utilized as Mios is required for oocyte maturation in invertebrate and vertebrate organisms that use sex chromosome based or multigenic sex determination mechanisms Since neither Tsc2 reduction nor Rhebca reproducibly suppressed oocyte loss in mios-/- we conclude that activating the metabolic stress arm of the mTorc1 pathway is not sufficient to support oocyte progression these results indicate that early oocyte development requires sufficient amino acid quantities to drive mTorc1 signaling and that oocyte progression is uniquely mediated by Gator2 and Mios We identify the mTorc1 pathway as an essential oogenesis pathway and a target of Rbpms2 activity We find that both Rbpms2 and Mios are required for successful nucleolar development and consequently ribosome biogenesis though they affect different stages of this process Rbpms2 acts early in nucleolar development likely regulating translation of targets involved in rDNA-mediated nucleation and/or rRNA seeding of nucleoli based on the impaired recruitment of the ribosomal RNA polymerase Normal recruitment of RNA pol I in mios-/- suggests that Mios nucleolar recruitment of pre-ribosome assembly components like Fibrillarin we provide genetic evidence that the relationship between DSB regulation and mTorc1 signaling observed in Drosophila is not conserved in zebrafish oocytes these results underscore the importance of ribosome biogenesis in oocytes and suggest that oocyte development past prophase I requires a certain threshold of ribosomes We hypothesize that if this minimum ribosome quantity is not attained in early zebrafish oocytes oocyte development is halted and remodeling of the gonad into a functional testis is triggered Through RNAIP and bulk RNA-seq experiments we provide evidence demonstrating the essential function of Rbpms2 as a multipronged regulator of sexual differentiation We observe few changes in overall RNA and rboRNA transcript abundance between wildtype and rbpms2 DMs which we show is present in nuclei of wildtype zebrafish oocytes up to diplotene arrest these results indicate that Rbpms2 likely regulates sexual differentiation through translational control of its target RNAs as RNA pol II-mediated transcription and overall mRNA abundance appear unperturbed in rbpms2 DM oocytes Further experimentation is required to determine if Rbpms2 similarly regulates rboRNAs as well as investigations into its potential binding partner(s) In this wild context the plasticity of the ovary and the Gator2-mediated checkpoint uncovered here could explain the observation of occasional ZW males in wild strains and WW males in lab strains thus reconciling the notion that the W is not sufficient for ovary fate Our results indicate the importance of Rbpms2 in ribosome biogenesis and further investigation is required to determine the relationship between Rbpms2 and rDNA amplification the available evidence suggests that in vertebrates the meiotic checkpoint and associated DNA integrity factors that safeguard faithful replication and reduction of chromosomes as part of meiosis operates independently of the mechanisms that ensure sex-specific gamete differentiation which in oocytes is orchestrated in part by mTorc1 signaling All procedures and experimental protocols were performed in accordance with NIH guidelines and were approved by the Icahn School of Medicine at Mount Sinai Institutional (ISMMS) Animal Care and Use Committees (IACUC #2017-0114) Fish were anesthetized with a lethal dose of tricaine (MS-22) and rostral-caudal body length was measured prior to dissection Trunks and gonads were prepared by removal of the head and tail regions exposure of the body cavity by opening the ventral body wall and removal of the gut tissue while leaving the gonads intact gonads were dissected from the trunk and reserved Trunks and gonads were preserved by flash freezing in liquid nitrogen and storage at −80 °C or fixation as described below lysed in 40 uL RIPA lysis buffer with protease inhibitor (Thermo Scientific Lysates were removed and stored overnight at −80 °C Sample lysates (18 uL) were then combined with equal volumes 2x SDS loading buffer and boiled for 5 min prior to loading 20 μl per sample were loaded on a 4-12% SDS-PAGE gel and proteins were transferred to PVDF membranes Membranes were blocked in 5% milk in 1x PBS for 1 h at room temperature 13557) was used at 1:250 and incubated overnight at 4 °C Membranes were washed for 5 min 3 times in 1x TBS-Tween and then for 5 min twice in TBS Rabbit-HRP secondary antibody (Cell Signaling Following a 1 h incubation at room temperature membranes were washed for 5 min 3 times 1x TBS-Tween and then for 5 min twice in 1x TBS Proteins were detected with an ECL-Plus kit (Cytiva Lifescience Chemiluminescence was imaged using a BioRad imager Nucleolar labeling and mTorc1 activity assessments were accomplished by blocking samples (1) and incubating with mouse anti-Fibrillarin (1:750 dilution; Novus Biologicals rabbit anti-p-p70s6k (1:500 dilution; Cell Signaling rabbit anti-Mios (1:500 dilution; Cell Signaling RNA pol I and II visualizations were accomplished by blocking (2) and incubating samples with rabbit anti-p-UBTF (1:100 dilution; Invitrogen PA5-105512) and mouse anti-RNA pol II (1:100 dilution; Santa Cruz sc-56767) and incubated at 4 °C for 30 h minimum samples were washed with 1xPBSTw several times and incubated at 4 °C for 24–48 h with chicken Alexa Fluor 488 and mouse Alexa Fluor 647 (Molecular Probes) secondary antibodies diluted 1:500 in (1) or 1x PBSTw (samples initially blocked in (2)) Samples were then washed in 1x PBSTw several times and mounted in Vectashield with 4′,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories) Prolong Diamond Antifade Mountant with DAPI (Invitrogen) or incubated briefly (30 s to 15 min) in DAPI and mounted in Prolong Diamond Antifade Mountant (Invitrogen) Images were acquired using a Zeiss Zoom dissecting scope equipped with Apotome.2 Zeiss Axio Observer inverted microscope equipped with Apotome.2 and a charged-coupled device camera or a confocal microscope Zeiss 880 AiryScan2.0 (×40 or ×63 oil objective 1024 × 1024 pixel format) at the Microscopy CoRE at ISMMS Image processing was performed in Zen Blue (Zeiss) The area of the nucleus was determined by finding the center of the nucleus and measuring its area from a single slice The puncta sizes were then normalized to the nuclear area of the given cell 750 µL YSS buffer was added to each homogenate and centrifuged Pellets were resuspended in 1 mL YSS buffer 250 µL of resuspended pellet was pre-cleared with 30 µL pre-washed protein G Dynabeads (Invitrogen 25 µL primary anti-RFP antibody (ChromoTek 5f8) diluted 1:500 in YSS was added to each 250 µL pre-cleared lysate and incubated overnight at 4 °C with rocking 30 µL pre-washed Dynabeads were added to each sample and rocked at 4 °C for 2 h magnetic beads were washed five times with YSS buffer resuspended in 100 µL Proteinase K lysis buffer (10 mM Tris HCl pH 8.0 0.5% SDS) with 10 µg proteinase K (New England Biolabs RNA was isolated using TRIzol (Life Technologies Precipitated RNA was stored at −80 °C until RNA library prep RNAs pulled down with mApple tagged Rbpms2 and mApple alone were compared from the cross-linked and uncrosslinked samples Only RNAs that appeared in both the cross-linked and uncrosslinked mApple-Rbpms2 samples but not in mApple control samples were considered Rbpms2 target RNAs (rboRNAs) 1-cell embryos were injected with 1 nL of plasmid solution (100 ng of plasmid 2.5 µL of Tol2 transposase RNA transcribed from pCS2FA-transposase and water (up to 5 µL)) and screened for mCherry heart expression at 1–3 dpf Progeny of these fish were then screened for germline transmission of the given transgene by mCherry heart expression and stable lines were propagated progeny from at least 2 independent crosses were evaluated and mutant n = 3) siblings and non-transgenic (mios wildtype n = 3 and heterozygous n = 3) siblings for both the ms49 and ms64 mTORca alleles were crossed to transgenic male siblings unrelated non-transgenic mios heterozygotes or mutants or non-transgenic unrelated SAT fish weekly for 4–5 (ms49 fish) or 3 (ms64 fish) crosses total All crosses were paired matings of individual males and females All eggs were collected within two hours of initial mating and the number of total (degenerating + fertilized + unfertilized) The fertility rate was calculated by dividing the number of fertilized eggs by the total number of eggs for a given cross and multiplying by 100 Differential Gene Enrichment Analysis was done using the DESeq2 package with adjusted P = 0.05 Sex ratio statistics were performed with a two-tailed χ2 test and a Bonferroni correction against stated groups in the associated figure legends All fluorescence and fertility assay quantifications were done with a two-tailed equal variance Student’s t test with a confidence interval of 0.95 between the stated groups in the associated figure legends We calculated study power using group versus population parameters for both dichotomous endpoint as well as continuous mean endpoints with a type I error rate of 0.05 and power of 90% and Investigators were blinded to allocation during the experiments and outcome assessment where applicable Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article Developmental control of oocyte maturation and egg activation in metazoan models rbpms2 functions in Balbiani body architecture and ovary fate Romano, S., Kaufman, O. H. & Marlow, F. L. Loss of dmrt1 restores zebrafish female fates in the absence of cyp19a1a but not rbpms2a/b. Development https://doi.org/10.1242/dev.190942 (2020) The dynamic regulation of mRNA translation and ribosome biogenesis during germ cell development and reproductive aging Metabolic control of oocyte development: linking maternal nutrition and reproductive outcomes DAF-2 and ERK couple nutrient availability to meiotic progression during Caenorhabditis elegans oogenesis Maternal diet-induced obesity alters mitochondrial activity and redox status in mouse oocytes and zygotes Stem cells and their progeny respond to nutritional changes during Drosophila oogenesis Rapid growth and out-crossing promote female development in zebrafish (Danio rerio) Amino acid-dependent control of mTORC1 signaling: a variety of regulatory modes Regulation of the mTOR complex 1 pathway by nutrients Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice Inhibition of S6 kinase by rapamycin blocks maturation of Rana dybowskii oocytes Hormone-induced meiotic maturation in Xenopus oocytes occurs independently of p70s6k activation and is associated with enhanced initiation factor (eIF)-4F phosphorylation and complex formation elegans AMPKs promote survival and arrest germline development during nutrient stress Makorin 1 is required for Drosophila oogenesis by regulating insulin/Tor signaling mTORC1 Signaling in oocytes is dispensable for the survival of primordial follicles and for female fertility TORC1 regulators Iml1/GATOR1 and GATOR2 control meiotic entry and oocyte development in Drosophila Ribosome biogenesis and cell growth: mTOR coordinates transcription by all three classes of nuclear RNA polymerases Liu, Y. et al. 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Genetics https://doi.org/10.1093/genetics/iyac016 (2022) Jang, S. et al. The Drosophila ribosome protein S5 paralog RpS5b promotes germ cell and follicle cell differentiation during oogenesis. Development https://doi.org/10.1242/dev.199511 (2021) Kong, J. et al. A ribosomal protein S5 isoform is essential for oogenesis and interacts with distinct RNAs in Drosophila melanogaster. Sci. Rep. https://doi.org/10.1038/s41598-019-50357-z (2019) Deng M., Wang X., Xiong Z. & Tang P. Control of RNA degradation in cell fate decision. Front. Cell Dev. Biol. https://doi.org/10.3389/fcell.2023.1164546 (2023) The RNA m 6 A reader YTHDF2 is essential for the post-transcriptional regulation of the maternal transcriptome and oocyte competence mRNA 3′ uridylation and poly(A) tail length sculpt the mammalian maternal transcriptome RNA degradation is required for the germ-cell to maternal transition in Drosophila mTORC1 signaling controls multiple steps in ribosome biogenesis Stops and starts in mammalian oocytes: recent advances in understanding the regulation of meiotic arrest and oocyte maturation The nucleolus as a multiphase liquid condensate Amplified ribosomal DNA in meiotic prophase oocyte nuclei of acipenserid fishes Morphological changes of the nucleolus during oogenesis in oviparous teleost fish Birth of a nucleolus: the evolution of nucleolar compartments Nucleation by rRNA Dictates the Precision of Nucleolus Assembly Fibrillarin: a new protein of the nucleolus identified by autoimmune sera mTOR signaling regulates the processing of pre-rRNA in human cells missing oocyte encodes a highly conserved nuclear protein required for the maintenance of the meiotic cycle and oocyte identity in Drosophila A systematic genome-wide analysis of zebrafish protein-coding gene function Heritable and precise zebrafish genome editing using a CRISPR-Cas system The ziwi promoter drives germline-specific gene expression in zebrafish A diverse array of cancer-associated MTOR mutations are hyperactivating and can predict rapamycin sensitivity Wei, Y. et al. The GATOR complex regulates an essential response to meiotic double-stranded breaks in Drosophila. eLife https://doi.org/10.7554/eLife.42149 (2019) mei-W68 in Drosophila melanogaster encodes a Spo11 homolog: evidence that the mechanism for initiating meiotic recombination is conserved Identification of novel Drosophila meiotic genes recovered in a P-element screen The telomere bouquet is a hub where meiotic double-strand breaks and stable homolog juxtaposition are coordinated in the zebrafish Zebrafish model of tuberous sclerosis complex reveals cell-autonomous and non-cell-autonomous functions of mutant tuberin Tsc2-Rheb signaling regulates EphA-mediated axon guidance Anomalies in human sex determination provide unique insights into the complex genetic interactions of early gonad development Females battle to suppress their inner male Physiological and pathological androgen actions in the ovary Zebrafish monosex population reveals female dominance in sex determination and earliest events of gonad differentiation Multiple sex-associated regions and a putative sex chromosome in zebrafish revealed by RAD mapping and population genomics An SNP-based linkage map for zebrafish reveals sex determination loci Wild sex in zebrafish: loss of the natural sex determinant in domesticated strains Direct male development in chromosomally ZZ zebrafish Zebrafish preserve global germline DNA methylation while sex-linked rDNA is amplified and demethylated during feminisation Conditional ablation of Raptor in the male germline causes infertility due to meiotic arrest and impaired inactivation of sex chromosomes Rapamycin-mediated mTOR inhibition impairs silencing of sex chromosomes and the pachytene piRNA pathway in the mouse testis Rapamycin improves the quality and developmental competence of in vitro matured oocytes in aged mice and humans A Guide for the Laboratory Use of Zebrafish (Danio rerio) Stages of embryonic development of the zebrafish Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT) Hermes RNA-binding protein targets RNAs-encoding proteins involved in meiotic maturation clusterProfiler 4.0: a universal enrichment tool for interpreting omics data clusterProfiler: an R package for comparing biological themes among gene clusters Grote, S. 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Biodock, AI Software Platform, Available from www.biodock.ai (2023) Download references We thank the members of the Marlow and Rangan labs for helpful discussions and The Center for Comparative Medicine staff at ISMMS for fish care Microscopy was performed at the Microscopy and Advanced Bioimaging CoRE at the Icahn School of Medicine at Mount Sinai Bulk RNA sequencing was performed at the New York University Langone’s Genome Technology Center (RRID: SCR_017929) We thank the Zebrafish International Resource Center for providing fish lines and Dr Wenbiao Chen for providing the mTOR L1460P and Rheb S16H pME constructs This work is supported by startup funds to F.L.M and the National Institutes of Health (R01-GM089979) was supported by a New York Stem Cell Foundation training grant (C32561GG) Work in the Draper lab was supported by R01 HD-081551 and NSF/IOS-1456737 to B.W.D Department of Molecular and Cellular Biology Department of Developmental and Molecular Biology The authors declare no competing interests Nature Communications thanks the anonymous reviewers for their contribution to the peer review of this work Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41467-024-49613-2 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing newsletter — what matters in science but enterprising restaurateurs across San Diego are saying YOLO and doing the damn thing in spite of it all is a love letter to the city’s culinary scene “This is about creating a place where people feel at home,” explains Teodulo the Mauricios developed a menu of classic Italian comfort foods emphasizing fresh pastas and house-made sauces Look for familiar favorites like lasagna alla bolognese with homemade pasta smothered with meat sauce and bechamel au gratin Their take on linguine ai frutti di mare spotlights all the best fruits of the sea in olive oil and tomatoes in a white wine marinara sauce (If there’s a better combination than garlic They’ll also serve plump gnocchi with melted mozzarella and fresh burrata; ravioli stuffed with lobster meat in a vodka cream sauce; cioppino made with calamari, shrimp, clams, mussels; and a fish of the day—I’ll have one of each, please. Beer and wine (mostly Italian) round out the menu, and there are also gluten-free options to accommodate a variety of diets Offering alternatives means all guests feel welcome The restaurant is now open in the former Sabor Brazilian Grill space with a short break between lunch and dinner View this post on Instagram A post shared by Finca (@finca.wine) Have breaking news, exciting scoops, or great stories about new San Diego restaurants or the city’s food scene? Send your pitches to [email protected] Beth Demmon is an award-winning writer and podcaster whose work regularly appears in national outlets and San Diego Magazine By clicking subscribe you're confirming that you agree with our Terms and Conditions Email: [email protected] By clicking Subscribe you’re confirming that you agree with our Terms and Conditions Email: [email protected] © Copyright 2023 San Diego Magazine 1230 Columbia Street MIO is undergoing a strategic review with assistance from Ardea Partners an investment bank founded by former Goldman Sachs executives US-based management consulting firm McKinsey is reportedly exploring the possibility of divesting its in-house asset manager MIO, which is responsible for the investment of private wealth for McKinsey’s senior staff and alumni, is currently undergoing a strategic review with the assistance of Ardea Partners, an investment bank established by ex-Goldman Sachs executives, reported Financial Times including a fine by the US Securities and Exchange Commission (SEC) in 2021 The SEC’s $18m fine addressed concerns over inadequate internal controls and the “potential” for conflicts of interest as partners involved with MIO’s investment decisions had access to sensitive client information McKinsey has since reformed MIO’s governance ensuring a clear separation between its consulting services and investment operations Its strategic review aims to align MIO’s relationship with the firm’s long-term interests and those of MIO’s clients Don’t let policy changes catch you off guard Stay proactive with real-time data and expert analysis The review will assess various strategic directions and alternative ownership structures with a focus on maintaining the continuity of MIO’s management governance rules aimed at preventing potential conflicts of interest have led MIO to refrain from investing in the individual stocks or bonds of any public or private company It instead engages in macro trading strategies These include trading in asset classes such as sovereign debt Give your business an edge with our leading industry insights View all newsletters from across the GlobalData Media network https://www.facebook.com/aydiosmiococina El Paso – El Paso is a very unique city encompassing and combining two cultures as a border town In the center of El Paso you’ll find the Five Points neighborhood and this section of the city now has a place for 5 star tacos called Ay Dios Mio.c Jennifer Mendoza owns this hot spot in a hot part of our state and she’s been in the restaurant business her whole life thanks to having a family full of eatery owners Ever since she can remember her family has owned restaurants as well as those of all her cousins and siblings Of course the one thing you can count on in El Paso is Mexican food so Jennifer wanted to do something different Even though the city has grown a lot recently Jennifer wanted to expand people’s horizons and not just be any old Mexican restaurant So she enlisted the help of Enrique Lozano a James Beard Nominated Chef with experience on shows like Chopped and Beat Bobby Flay bonding both as business partners and as friends Together they teamed to create a restaurant known for tacos but these are far from your ordinary tacos they wanted to push the boundaries of what it means to have food at a bar Enrique got things started on a massive meal consisting of pretty much every taco on the menu with a side of bone marrow Some fresh cod gets tempura fried as everything on the grill gets hit with a house-made seasoning salt consisting of 25 different spices The tripas get caramelized onion and cilantro The octopus is sliced up with fried baby potatoes It’s almost like taking the finest part of a Wagyu beef steak and cooking it in butter tripas and even the vegetarian tacos were all incredible as well each with their own unique flavor combinations The carne asada taco is probably the most classic dish on the menu but it still stands out just based on how well it’s done for an incredible smorgasbord of fabulous fun tacos coming to the Sun City and saying ay dios mio at Ay Dios Mio is definitely well worth a stop on The Texas bucket list Reviews and a host of other European artistic luminaries is a cinema in-joke elongated beyond all reason one with a pedigree of sorts; Honoré and Mastroianni embarked on this movie after having already collaborated on a half-dozen films.  Chiara plays herself, as does most of the other cast. We first see her in a sleeveless black dress and blonde wig, romping in the aforementioned Trevi Fountain, recreating a scene from Fellini’s “La Dolce Vita,” in which Anita Ekberg out of costume in a hotel room with her mother Chiara looks in the mirror and sees a CGI image of her father looking back at her I have dad’s face,” Chiara says to her mother who responds noncommittally but with affection.  Soon after that, on a film set, she takes direction from Nicole Garcia, who says of her work in a scene, “I’d prefer you play it more Mastroianni than Deneuve.” A little later, a colleague observes, quoting Nietzsche, “Everything good in life is inherited.” Not helpful, colleague. In Polanski’s “The Tenant,” the title character Trelkovsky went mad because her fellow apartment dwellers were Chiara decides to roll with folks trying to turn her into Marcello Her dad’s identity becomes a crutch (actor Melvil Poupad calls her out on that)—or a rut in which she can luxuriate; whatever you call it it’s a means for Ciara to avoid her actual self Regarding Marcello’s inability to fully embrace monogamy “That’s what Faye couldn’t understand.” “Faye?” asks her companion the actress being one of Marcello’s more storied loves.  Before this journey of identification is over Mastroianni will appear on an Italian talk show in full Marcello drag and sing a song to a shaggy dog on a stool while another Italian screen icon If you know about Italian talk shows this won’t seem too weird…but it’s still a little weird But not weird enough to rescue the film from the longueurs that often attend a vanity project which is what this picture is whether it was Honoré or Mastroianni who initiated it.  and this moving conclusion finds a grace that the movie Glenn Kenny was the chief film critic of Premiere magazine for almost half of its existence. He has written for a host of other publications and resides in Brooklyn. Read his answers to our Movie Love Questionnaire here Mary girls basketball team found themselves in almost the same exact situation Coming off a relatively easy road to a Division 4 district championship GSM had to go head-to-head with the state's top scorer in Mia McGregor and the Mio-Au Sable Thunderbolts GSM did everything they could to slow down the 38 points per game scorer throwing double and triple teams at her anytime she touched the ball despite the sophomore phenom ending the game with 36 points it was the Snowbirds that ended up on top of the scoreboard as they finished off the 50-48 victory Districts: St. Mary girls basketball wins fifth-straight district title, will face Mio in regionals HS: Harbor Springs’ season ends in Division 3 regional semi our top three girls worked their butts off tonight," said coach Pat Schultz Snowbirds throw everything at McGregorComing up with a defensive gameplan for a scorer like Mia McGregor already Mio's career point leader after just two seasons in high school basketball his hope was to let anyone other than the star sophomore beat them Mary dedicated three defenders to McGregor at most times not allowing her to get free via any on- or off-ball screens and making every shot difficult she found ways to breakthrough that staunch Snowbirds defense finishing with a game-high 36 points at the end of the game you have to commit three girls to one girl and she still scores 36 points," said Schultz "That just tells you what kind of player she is." especially in the first half as they held her to 11 points with a one-point lead and the game on the line the Snowbirds defense did what it needed to as a double-team from Allyra Cook and Kaylee Jeffers forced a McGregor turnover sending Jeffers to the line with just 2.4 seconds remaining Payton Glasby led the Snowbirds with 14 points while Jeffers added 13 Beating Mio was just the first step in the Snowbirds regional finals plan as the win sets up a rematch with last year's regional champions A sophomore-led Snowbirds team had a lot of trouble with Frankfort's post offense last season while Frankfort still has the size mismatch on GSM battle-tested through the first few games of the playoffs They worked hard to put themselves back in a position to play one of these teams again," said coach Schultz Contact GHT Sports Editor Dylan Jespersen at Djespersen@gaylordheraldtimes.com (WPBN/WGTU) - - A 23-year-old woman is now facing six felony counts for allegedly sexually assaulting a minor in 2022 The suspect was identified as 23-year-old Aray Colvin of Mio In October 2022 the Oscoda County Sheriff’s Office investigated an alleged criminal sexual conduct complaint involving a minor after the parent of the victim reported the alleged abuse Deputies said the case involved multiple sexual assaults against the victim who is a minor An investigation occurred and the victim was interviewed at the Northern Michigan Child Advocacy Center in Roscommon MSP said the report was turned over to the Oscoda County Prosecutor’s Office and a six-count felony warrant was issued on January 22 Troopers contacted the MSP Seventh District Fugitive Team to assist with the arrest of Colvin deputies said Colvin was arrested and lodged in the Oscoda County Jail Colvin was arraigned in the 81st District Court in Oscoda County on February 14 on a six-count felony warrant: The release said she was given a $30,000 10% cash surety bond Colvin is to be seen in court on February 27 a leading provider of chat interoperability solutions is excited to announce a new partnership with Gapps a renowned digital transformation consultancy with deep expertise in Google Workspace This partnership aims to enhance cross-platform communication capabilities for enterprises leveraging both Google Workspace and Microsoft 365 creating a seamless and collaborative environment for all users With Mio’s advanced interoperability solutions enterprises can now ensure that their employees using Google Workspace can communicate effortlessly with colleagues using Microsoft Teams This integration is especially valuable for businesses managing hybrid environments allowing them to unify communication channels without requiring users to switch tools Gapps has an established reputation for guiding organizations through transformative projects equipping them with tools that boost productivity and collaboration Gapps will now offer Mio’s interoperability solutions to address a growing need for seamless multi-platform communication businesses can benefit from key interoperability features such as: This partnership brings together Mio’s leading-edge interoperability technology and Gapps’s extensive experience in digital transformation empowering organizations to break down communication silos and support efficient To learn more about how Mio and Gapps are unifying workplace communication, visit www.m.io or reach out to our team Gapps is a leading digital transformation consultancy specializing in Google Workspace, dedicated to helping organizations optimize their productivity and collaborative capabilities - securely. With a focus on innovative solutions, Gapps drives successful digital transformation projects across diverse industries. Learn more at www.gappsgroup.com Mio powers effective communication across multiple messaging platforms by enabling users to collaborate seamlessly. Founded in 2016 in Austin, Texas by Tom Hadfield and James Cundle, Mio has secured $17 million in funding from major investors, including Zoom, Cisco, and Y Combinator. For more information, please visit www.m.io Please enable JS and disable any ad blocker (WPBN/WGTU) - - The Mio-AuSable Schools have decided to cancel schools on Friday December 20 due to a third consecutive day of threats a staff member was informed that a student made a threat The student was immediately escorted to the office and the school’s Resource Officer engaged in a thorough search Along with the students from the previous day’s incident they were taken into custody of the police and the school said the appropriate legal consequences will be made “Law enforcement has informed us that all three incidents over the past few days are separate,” said Gary Wood All 6th-12th grade students were brought to be informed of the serious consequences for making threats by the county prosecutor and School Resource Officer “We want to make it clear that any student who makes threats will be prosecuted to the full extent of the law,” said Gary Wood the Mio-AuSable Schools have decided to close their schools for Friday December 20 along with any extracurricular activities and the elementary concert This also goes for any extracurricular activities including after-school activities for Thursday a police officer will remain present on campus There are no statistics available for this player Thanks for visiting The use of software that blocks ads hinders our ability to serve you the content you came here to enjoy We ask that you consider turning off your ad blocker so we can deliver you the best experience possible while you are here (WPBN/WGTU) -- (UPDATE) -- Mio-AuSable Schools said the student responsible for posting a threat that led to a lockdown has been removed from the school and taken into law enforcement custody The school said the lockdown on Wednesday ended at 12:45 p.m Staff said Wednesday’s lockdown is unrelated to another lockdown on Tuesday and law enforcement is treating it as a separate matter and there will be a visible police presence on campus (WPBN/WGTU) -- Mio-AuSable Schools are on lockdown and a student is being interviewed by law enforcement after the school said the student “posted a picture of a gun with a message suggesting a potential school shooting.” both the student and their locker have been thoroughly searched Multiple law enforcement officers are on-site and a K9 unit is currently conducting a search of the building The school went into lockdown on Tuesday because “a student overheard another student mentioning a potential threat involving a shooting.” The school said that the two were unrelated issues If you’ve played a Hazelight Studios game before you know that the characters they create for you and your co-op partner come with unique personalities and motivations that produce unforgettable moments as their stories unfold.  Hazelight Studios is keeping that trend up as they introduce two new player characters in Split Fiction who you will quickly find are worlds apart they have been lured into Rader Publishing Headquarters with the promise of a potential publishing deal which goes horribly wrong trapping them inside a simulation of their stories which you have to help them escape we’re diving deeper into their characters so that you can confidently pick your favourite when you get your hands on the game!  Mio has barely scraped by for the last couple of years She isn’t looking for fame or recognition as an author Her love for all things sci-fi is reflected in her literary works Expect to see her love for sci-fi shine through in the abilities she gains inside the simulations where she may become a gravity-defying ninja equipped with a lethally sharp energy sword wear an advanced exosuit that grants remote control of drones or dual-wield laser blasters in futuristic combat armour A cheerful countryside girl with a deep need to prove to her family that she’s not a complete failure Zoe hopes that a publishing deal will solve all of her problems with her portfolio containing works of fiction about escaping towns overrun by trolls battling through forests against an impending ice age and protecting the last dragons from extinction you can look forward to seeing Zoe as a magical shapeshifter flying across the lands as a fairy wielding a mythical sword whilst riding a dragon into a battle of the ages or soaring between floating islands with a magical kite As our characters are clearly such polar opposite personalities they have to come to terms with their differences and realise that to escape the simulations they find themselves trapped in they must work together and overcome the odds through the power of friendship During the game announcement at The Game Awards in December Josef Fares revealed that the characters’ names mean a great deal to him as Mio and Zoe are in fact named after his daughters Now for the tricky question for you and your co-op partner: who is going to play as Mio and who will play as Zoe… allowing one player who owns the game to invite a friend to play online for free Hazelight also plans to introduce crossplay making playing with friends even easier across any platform.  Split Fiction is coming to PlayStation 5 on March 6 *Friend’s Pass requires installation of the Friend’s Pass persistent internet connection and applicable platform account One user must own the full game in order for the co-op player to play the full game Learn more Hillman clinched the Little Dipper North Star League Championship with a convincing 59-44 win over Mio on Friday The league championship is Hillman’s fourth consecutive title and 10th title in the last 15 years The game was a battle of the two top teams in the division with Hillman leading by one at halftime The second half was a different story as Hillman outscored Mio 14-3 in the third quarter and outscored Mio 29-15 in the second half Junior wing Aedan Tucker had a big night scoring a season-high 19 points and junior center Evan Crane added 18 points and six boards Senior Brenden Rouleau added five steals to go with his nine points and senior point guard Cuyler Schultz led the team in assists with eight and also added six rebounds The conference title was a nice addition to an already impressive resume being a part of three districts “What a great victory tonight!” Hillman coach Eric Muszynski said on Friday “Always nice to send your seniors out in the right way playing in their last NSL home game We played so well tonight and everyone contributed and I’m proud of my guys and happy for the community!” Today's breaking news and more in your inbox Copyright © 2025 Alpena News Publishing Company | https://www.thealpenanews.com | 130 Park Place This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page we are discussing the madness that occurred at the Ft Lauderdale Pro Swim Ledecky’s world record in the 800 free proves that age is seemingly just a number for her but it also makes the Rio 2016 swim she beat out more memorable Marchand has a few areas of improvement to work on if he hopes to return to his Paris form at the World Championships this summer in Singapore March 28th, 2025 Asia, International, News, Previews & Recaps, Records the sole qualifying opportunity for this summer’s World Championships the Tokyo Aquatics Centre is playing host to the 47th National Junior Olympic Cup Spring Swimming Championships The competition spans four days and includes short course meters prelims and finals for girls/boys ages 11-12 Already qualified for Singapore, 18-year-old Mio Narita was in the water once again Narita stopped the clock at a time of 4:28.19 to handily take the gold establishing a new Japanese National High School Record in the process Splits for Narita’s new JPN HS Record: Entering this competition, Miku Kojima held the high school standard so Narita managed to shave .30 off to check in with the new mark Olympic finalist Narita’s performance here also represents a new lifetime best overtaking her previous PB of 4:29.70 notched at the 2022 Japanese Short Course Championships She now becomes Japan’s 4th-quickest SCM 400 IM performer ever Top 5 Japanese Women’s SCM 400 IM Performers All-Time 17-year-old Kazushi Imafuku is also racing at this competition making his presence known in the 400m freestyle Imafuku stopped the clock at a time of 3:42.44 to establish a new meet record with Imafuku’s outing checking in as a new personal best by over 4 seconds He’s now Japan’s 9th-swiftest SCM 400 free performer of all time Δdocument.getElementById( "ak_js_1" ).setAttribute( "value" Δdocument.getElementById( "ak_js_2" ).setAttribute( "value" Former Masters swimmer and coach Loretta (Retta) thrives on a non-stop but productive schedule that includes having earned her MBA while working full-time in IT while owning French 75 Boutique while also providing swimming insight for BBC More from Retta RaceSee All Subscribe to our newsletter and receive our latest updates Website: ecomio.com STARTUP STAGE: Eco.mio helps companies guide employees to choose greener travel options Sustainable business travel startup Eco.mio raises 7-figure seed round  Mary Snowbirds beat the Mio Thunderbolts 50-48 in the regional semifinal The game came down to the final seconds with the Snowbirds getting a big steal to end it Mary will play Frankfort on Wednesday for a spot in the quarterfinal multi-platform environments have become the norm for modern enterprises To address the global need for cross-platform collaboration we are excited to announce our partnership with Cloudpoint a leading Finnish company specializing in Google Cloud services Cloudpoint now offers Mio’s middleware solution providing native bi-directional chat and file sharing interoperability between Google Workspace and Microsoft 365 or Slack Cloudpoint’s expertise in Google Cloud services and application development and Mio’s full suite of interoperability features are well suited to deliver an interoperability solution to the Finnish market Mio is the only middleware solution that enables bi-directional chat and file sharing interoperability through the synchronization of user directories for enterprises using Google Workspace enterprises around the world can keep their employees happy by eliminating work silos and providing them with a frictionless collaboration experience Cloudpoint is one of the oldest Google Cloud Partner and technology service provider in Finland They act as a trusted partner that guides Finnish municipalities and organizations through their digital transformation journeys Their agile approach guarantees tailored solutions empowering every customer to overcome their specific challenges and achieve their goals By combining their deep understanding of technology with their data protection and security expertise Cloudpoint empowers their clients to confidently embrace the technology while safeguarding their valuable information To learn more about how you can enhance collaboration and connectivity within your organization, visit Mio and Cloudpoint's website We’re thrilled to announce our latest partnership with Brio a cloud consulting services provider based in India Brio will now offer Mio’s bi-directional native chat interoperability solution for businesses using Google Workspace and Microsoft 365 Brio’s expertise in IT services and digital transformation perfectly complements Mio’s mission to deliver bi-directional chat and file-sharing interoperability companies around the world can now seamlessly connect their Microsoft and Google environments ensuring a smooth collaboration for all of their employees Mio is the only middleware solution that enables real-time interoperability for teams using Microsoft Teams and Google Chat businesses can connect employees across platforms without worrying about managing multiple accounts or licenses Brio has built a reputation as a trusted IT partner in India offering tailored solutions to help organizations achieve their digital transformation goals they can now offer even more value to businesses looking to optimize their workflows and eliminate work silos To learn more about how you can bridge the gap between Microsoft and Google Workspace, visit Mio’s website The 2025 Japan Swim kicked off today from the Tokyo Aquatics Centre site of swimming action at the 2020 Olympic Games One of the marquis events on day one was the women’s 200m IM and Mio Narita ensured her presence was known to kick off her campaign 18-year-old Narita cranked out a time of 2:09.68 to beat the field by nearly a second en route to capturing the gold Shiho Matsumoto was next to the wall in 2:10.61 while Shuna Sasaki rounded out the podium in 2:11.80 Narita’s result represents a new lifetime best for the teen overtaking her previous PB of 2:10.11 logged at last year’s Konami Open for her first-ever foray under the 2:10 barrier Narita’s performance tonight established a new Japanese National High School Record Three-time Olympian Rikako Ikee held the previous benchmark with the 2:09.98 she put on the books in 2017 Splits for Narita this evening included 28.35/33.52/36.90/30.91 to become Japan’s 5th-swiftest performer of all time Top 5 Japanese Women’s LCM 200 IM Performers All-Time Narita’s performance now inserts her into the season’s world rankings in slot #2 Her new high school record also easily cleared the 2:12.83 World Aquatics ‘A’ standard needed for this summer’s World Championships Matsumoto’s silver medal-worthy outing of 2:10.61 also made the grade After the race, Narita told Nikkan Sports “It’s [breaking the record] always been my goal “I want to do my best to get a personal best of 4 minutes 34 seconds.” The Eagles will take on Traverse City Christian on Thursday On Wednesday morning, 8 January 2025, BepiColombo successfully flew past planet Mercury for the sixth time Mio and MTM got as close as 295 km to Mercury’s surface The trio approached the planet on its cold night side after which they flew over shadowed craters at Mercury’s north pole and the sunlit northern volcanic plains They filmed the surface using MTM’s monitoring cameras and both Bepi and Mio used their science instruments to investigate the little planet’s surroundings.   This sixth flyby is the last time the trio greet the planet together MTM will wave goodbye to its two orbiter buddies as they approach Mercury again in late 2026 after which Bepi and Mio will settle into separate orbits around their planet friend We expect to release the first images from today's flyby on 9 January with other scientific data to follow.  Click here for an overview infographic of the flyby   Meet the cartoon characters during their mission training  Vision & Voice for the Advisory Community 30 London newspaper exposé.css-1sf3xto{padding-top:16px;padding-bottom:16px;}.css-13ibk4z{margin:0;font-family:"Lato",Helvetica,Arial,sans-serif;font-weight:400;font-size:1rem;line-height:1.5;}The New York super-consultancy retained Ardea Partners to suss out its options for the in-house wealth manager and hedge fund company McKinsey (1) Comments.css-c4sutr{height:auto;overflow:visible;-webkit-transition:height 300ms cubic-bezier(0.4 1) 0ms;transition:height 300ms cubic-bezier(0.4 1) 0ms;transition:background-color 300ms cubic-bezier(0.4 1) 0ms;cursor:unset;outline:0;-webkit-text-decoration:none;text-decoration:none;border:0;padding:0;vertical-align:middle;box-sizing:border-box;}.css-zza5d7.Mui-disabled{opacity:0.38;pointer-events:none;}.css-zza5d7 .MuiChip-avatar{margin-left:5px;margin-right:-6px;width:24px;height:24px;color:#616161;font-size:0.75rem;}.css-zza5d7 .MuiChip-avatarColorPrimary{color:#fff;background-color:rgb(11 120);}.css-zza5d7 .MuiChip-avatarColorSecondary{color:#fff;background-color:rgb(119 33);}.css-zza5d7 .MuiChip-avatarSmall{margin-left:4px;margin-right:-4px;width:18px;height:18px;font-size:0.625rem;}.css-zza5d7 .MuiChip-icon{margin-left:5px;margin-right:-6px;color:inherit;}.css-zza5d7 .MuiChip-deleteIcon{-webkit-tap-highlight-color:transparent;color:rgba(255 0.7);font-size:22px;cursor:pointer;margin:0 5px 0 -6px;}.css-zza5d7 .MuiChip-deleteIcon:hover{color:rgba(116 0.4);}.css-zza5d7 .MuiChip-deleteIcon:hover,.css-zza5d7 .MuiChip-deleteIcon:active{color:#fff;}.css-9iedg7{overflow:hidden;text-overflow:ellipsis;padding-left:12px;padding-right:12px;white-space:nowrap;}January 30 2025 — 7:09 PM Another likely motivator here is McKinsey's Dec 13 2024 $650M settlement with the DOJ for McKinsey's role in the Purdue Pharma / Opioid issue Presumably MIO is owned by the McK partnership and selling it off is a great way to raise the $650M vs asking current partners to come out of pocket or take on debt...Post a comment.css-a0y2e3{height:0;overflow:hidden;-webkit-transition:height 300ms cubic-bezier(0.4 68);}.css-y8313w{margin:0;font-size:1.2rem;font-weight:600;color:rgb(77 The New York City consulting giant quietly made the Orion founder and ex-CEO a McKinsey senior advisor two months after he stepped down last year. California Copyright © 2009-2025 RIABiz Inc Service Staatliche Museen zu Berlin One of Okido’s starting points are neo-traditional national-Japanese Nihonga paintings from the exhibition Contemporary Japanese Painting Four of them are on display in Room 318 Okido confronts these paintings with motifs relating to Japan’s military aggression in Manchuria in the same year The two-channel video installation Betrachten (Viewing)creates a complex visual atlas of memory by combining historical images and documents with footage of places and works of art as carriers of contradictory emotions and interests taken by Okido herself during a trip to Korea and Japan in 2023 All the images appear in black and white in uniform format and the constructed character of the narratives of (hi)stories associated with the images Fassade des Gesichts (Facial Façade) shows a portrait of the artist with a veil of medallions who are regarded as heroes of Japan’s modernisation but appear here in miniature and the form chosen by Okido The wall-based textual sculpture Menschliche Beziehungen (Human Relationships) forges a bridge to the German language and links the topicality of the subject to Berlin and the world of today Condensing the remembered narratives of (hi)stories into “killing” or “being killed” the artwork poses a central question for the present and (hi)stories of the future The exhibition is curated by Kerstin Pinther curator for modern and contempary art in global context Ethnologisches Museum and Museum für Asiatische Kunst in cooperation with the artist and the curator for Arts of Japan A special exhibition of the Museum für Asiatische Kunst – Staatliche Museen zu Berlin under the auspices of the project CoMuse U-Bahn: FriedrichstraßeS-Bahn: Friedrichstraße, Hackescher MarktTram: Am Kupfergraben, Hackescher MarktBus: Staatsoper, Lustgarten, FriedrichstraßeLow-carbon public transport connectionsLocal trafficLong distance Sun 10:30 - 18:30Mon 10:30 - 18:30Tue closedWed 10:30 - 18:30Thu 10:30 - 18:30Fri 10:30 - 18:30Sat 10:30 - 18:30 Special opening hours during public holidays Press release Mio Okido als Fellow im Projekt „Das kollaborative Museum“ (CoMuse) Exhibition brochure (PDF, 1.1 MB) On Sunday, 1 December 2024, BepiColombo successfully flew past planet Mercury for the fifth time Mio and MTM greeted the small planet from just 37 626 km away at 15:23 CET Bepi used its infrared camera (called MERTIS) to take unusual pictures of the planet Rather than showing what the surface looks like in normal visible light MERTIS will reveal which parts of the surface are hotter than others and hint at what kinds of minerals the surface is made of. In the coming week we will reveal what Bepi's flyby image shows Mercury won't have to wait long until it can wave at the trio once again – their next visit will already happen at the start of the new year Each time the trio passes by the planet, Mercury's gravitational pull changes their speed and direction. All of this prepares the spacecraft for late 2026 when over four months the three parts of the spacecraft will separate in turn and Bepi and Mio will each settle into orbit around their planet friend.   Click here for an overview infographic of the flyby  Meet the cartoon characters during their mission training Mio girls basketball coach Kristi McGregor talks strategy with her team during its game at Hale.Provided by Mark Pijaszek Mio got the rematch it wanted but not the result Mary knocked the Thunderbolts out of tournament play prevailing 50-48 in the Division 4 regional semifinal at McBain Mia McGregor delivered a triple-double with 36 points 10 rebounds and 10 blocked shot for Mio (18-6) The sophomore sensation finished the season with a 39.0 scoring average for the Thunderbolts Serenity Shattuck added six points and Lilly Strach pulled down six rebounds for Mio which captured North Star League Little Dipper and district championships this season Mio made a similar run a year ago before being edged by St Mary (21-3) now has a much-anticipated rematch of its own taking on Frankfort in the regional final at 7 p.m Clare hit a regional roadblock for the second year in a row falling to a powerful Fremont squad 67-43 in the Division 2 regional semifinal The Pioneers (16-8) were rolling after winning their second straight district championship hitting three 3-pointers in the final minute of the first half to take charge Tessa Kelly scored 17 points for Clare and Kendra Fouts added 12 Riley Chase poured in 29 points for Fremont Use of and/or registration on any portion of this site constitutes acceptance of our User Agreement, (updated 8/1/2024) and acknowledgement of our Privacy Policy, and Your Privacy Choices and Rights (updated 1/1/2025) © 2025 Advance Local Media LLC. All rights reserved (About Us) The material on this site may not be reproduced except with the prior written permission of Advance Local Community Rules apply to all content you upload or otherwise submit to this site YouTube's privacy policy is available here and YouTube's terms of service is available here Ad Choices Service During the course of her artist residency from October to December 2023 as part of the Collaborative Museum project organised by the Museum für Asiatische Kunst and the Ethnologisches Museum Japanese artist Mio Okido conducted research into the exhibition Contemporary Japanese Painting which was held at the Akademie der Künste Berlin in 1931 She also explored neo-traditional Japanese painting (Nihonga) and addressed the subject of Japanese imperialism and colonialism in the 19th and 20th centuries The central theme of Mio Okido’s artistic practice is the construction of memories images and other evidence of visual culture monuments and (memorial) sites are employed by individuals institutions and nation states to construct versions of (hi)stories that serve their own specific interests Mio Okido says of her creative approach: “Japanese people always want to project a positive view of their country to the Western world which frame it as a beautiful and exotic land The reality is that during the 19th and 20th centuries Japan’s development was also driven by imperialism and colonialism – a historical truth that is seldom addressed the question of Japan’s historical responsibility still remains As long as the Western world continues to cling to its pretty In the exhibition Remembered Images, Imagined (Hi)Stories – Japan, East Asia and I (14 September 2024 to 3 February 2025 in the Humboldt Forum) Mio Okido showcases new artworks that were created during her artist residency Artist talk with Mio Okido in the Humboldt Forum on 31 January 2025 COMMUNITY PARTNER Stay connected to All Things Good in the Burg by subscribing today Since I Love the Burg launched in May of 2009