Between carnival and Mardi Gras, March is a month full of festivities, especially in Parmain, in the Val-d'Oise département where the2025 edition of the town's carnival takes place on Saturday March 15 Get together with family and friends at 2 p.m to enjoy the entertainment - face-painting - before setting off for the parade and parading through the streets of the town in your best costumes and accessories accompanied by majorettes and a brass band before the traditionalfireworks display by Monsieur Carnaval The carnival and the grand parade are open to all: all you have to do is join in and enjoy the festive atmosphere of Parmain's great popular festival!The Parmain 2025 carnival route: Refer your establishment, click here Promote your event, click here (WATCH) Trump proposes reopening of infamous Alcatraz prison amid illegal immigration crackdown Peru kidnapping leaves 13 dead in gold mine Vatican to send retired Popemobile to Gaza as an ambulance Cardinals hold final mass mourning Pope Francis ahead of conclave one missing after tourist boats capsize in China Media as the Bridge of Civilizations: A Global Call for Integrity and Cooperation Kenya-China: A Bold Blueprint for a Shared Future HESBON OWILA: Kenya Kwanza’s Dwindling Promise — and a Thin Thread of Hope Safeguarding Lives and the Economy: Why Product Integrity Must Be a National Priority Victor Bwire: It’s Time Media Took Responsibility for Its Rotten Core Uganda sign historic MoU to protect Mt Elgon biosphere reserve Climate change threat to Kenya’s national security CS Duale Urges Corporate Sector to Support Ecosystem Protection Emissions from building sector stopped rising for the first time since 2020 Greenpeace Africa Slams Kenya Forest Service for Downplaying Forest Threats Kenya to open consulate in Haiti to support peace mission Kindiki in Uganda for Extraordinary Summit on Somalia Peace Mission Wetang’ula to represent Kenya at Pope Francis’ funeral in Vatican President Ruto lands in Beijing ahead of 3-day State Visit Chinese enterprises in Kenya lead green growth Over 50,000 in China’s Hainan evacuated as Typhoon Trami grows – China Daily Over 60% of China’s population proficient in primary or higher digital skills – China Daily China urges US to cease arming Taiwan: FM spokesperson – China Daily 5.5-magnitude quake hits China’s Xinjiang: CENC – China Daily Education Cabinet Secretary Julius Migos Ogamba revoked the appointments of Sally Ngeringwony Toroitich Dr Parmain Ole Narikae and Carren Kerubo Omwenga Apr 14 — The Ministry of Education has moved to shake up the leadership of the University of Nairobi after revoking the appointments of four members of the university’s council Click here to connect with us on WhatsApp Education Cabinet Secretary Julius Migos Ogamba revoked the appointments of Sally Ngeringwony Toroitich, Ahmed Sheikh Abdullahi, Dr Parmain Ole Narikae and Carren Kerubo Omwenga. The revocation, made under Section 36(1)(d) of the Universities Act, 2012 and Section 51(1) of the Interpretation and General Provisions Act, comes amid growing scrutiny of the university’s leadership. This latest move follows a similar decision in December 2024, when the Cabinet Secretary revoked the appointment of Joel Kibe as a council member. Kibe, who had been appointed for a three-year term by former Education CS Ezekiel Machogu, was removed just over a year into his tenure — with no explanation provided. The revocations are unfolding against a backdrop of deepening governance challenges at Kenya’s top university. In December 2024, the National Assembly’s Education Committee began investigating the University Council over alleged mismanagement and leadership controversies. Among the issues raised was the contentious removal of former Vice-Chancellor Prof. Stephen Kiama and the findings of a damning probe by the Ethics and Anti-Corruption Commission (EACC). According to a letter dated October 4, 2024, the EACC accused the council of illegally creating 14 administrative positions — including that of Chief Operations Officer (COO) — without amending the university’s charter as required by law. The Commission declared these roles “illegal, null, and void” for failing to comply with Section 22A of the Universities Act. The EACC directed the council to take corrective actions within 30 days and to resolve pending court cases related to the administrative overhaul. During a December 3 parliamentary hearing, MPs accused the council of undermining the institution’s governance and creating instability by appointing unqualified individuals and bypassing legal procedures. While the Education Ministry has not yet announced replacements for the recently dismissed council members, expectations are high amid sustained efforts to restore integrity and accountability at the institution. As scrutiny intensifies, the spotlight remains firmly on the Ministry and the Council’s next steps in steering the University of Nairobi out of what many see as a prolonged leadership crisis. The fourth-year student Bernard Wangila was set free due to a lack of evidence In October 2024, the University Council, announced the termination of Kiama’s appointment as Vice Chancellor of the University of Nairobi and named Prof. Margaret Hutchinson as... No explanation was provided for the revocation, adding to the ongoing controversies surrounding the leadership of the council and the university. He said the management of the University must rescind the decision noting a number of students have passed through the Campus successfully. Council Chairperson Professor Amukowa Anangwe Wednesday asserted the decision to send Vice Chancellor Prof Stephen Kiama on leave, further saying a directive reversing administrative... Prof. Kiama's return came hours after UoN's teaching staff demanded his immediate reinstatement following a puzzling departure on August 1. A source told Capital News that the directive followed an acrimonious university reform fallout. ARUSHA, Tanzania Oct 24 – Antony Manyara has been sworn in as the president of East African Student’s Union (EASU). Manyara took oath last... This website is using a security service to protect itself from online attacks. The action you just performed triggered the security solution. There are several actions that could trigger this block including submitting a certain word or phrase, a SQL command or malformed data. You can email the site owner to let them know you were blocked. Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page. Volume 7 - 2019 | https://doi.org/10.3389/fevo.2019.00495 This article is part of the Research TopicDNA Barcodes: Controversies, Mechanisms and Future ApplicationsView all 13 articles Saproxylic beetles are important bioindicators of forest health but their enormous diversity makes their identification challenging the French fauna of saproxylic beetles alone contains 2,663 species in 72 families DNA barcoding was proposed as a promising tool for the identification and monitoring of saproxylic beetle species the rate of DNA barcode recovery from specimens of natural history collections using standard Sanger sequencing protocols remains low and challenges the construction of reference libraries we test the potential of high-throughput sequencing (HTS) technology to reduce this shortfall by increasing sequencing success rate and lowering processing cost per specimen Using a dual-indexing strategy for library construction and sequencing on the Illumina MiSeq platform we successfully sequenced the DNA barcodes of 286 dry-pinned saproxylic beetles out of 521 specimens aged from 1 to 17 years and sampled in natural history collections Age at sequencing did not affect sequence recovery and the success rate (54.9%) of our approach is comparable to that obtained using Sanger sequencing technology in another study targeting beetle specimens from natural history collections but the cost per specimen is significantly reduced when using HTS we shortly discuss how the newly produced DNA barcodes contribute to the existing library and we highlight a few interesting cases in which the new sequences question current species boundaries covering more than 2,100 European saproxylic beetle species so far These libraries revealed the general consistency between morphologically characterized species and DNA barcode clusters and thus supported the relevance of this genetic marker for delimitating and identifying beetle species because sequencing of degraded DNA requires the amplification of shorter amplicons it seems appropriate to adapt and use HTS to process collection specimens toward sequencing of DNA barcodes Our main aim was to extend the taxonomic and geographical coverage of the French saproxylic beetle DNA barcode library while testing the benefits of using HTS technology when processing specimens whose DNA is expected to be degraded We focused our sampling on species from the French Pyrenees where we are carrying out a metabarcoding analysis of forest biodiversity (CLIMTREE project) Tissue samples were placed in 96-well plates for which an abdomen was taken after genitalia removal due to the lack of significant diagnostic characters for taxonomic identification and the higher amount of tissue it provides Sampling was done using sterilized forceps Collecting data were compiled into a standard Darwin Code spreadsheet and vouchers were photographed using either a 14MP 1/2.3″ APTINA CMOS Sensor U3CMOS mounted on a stereomicroscope or a Nikon D7200 with an AF-S DX NIKKOR 18-300MM F/3.5-5.6G ED VR Lens for the biggest individuals DNA extraction of 521 individuals belonging to 343 species and 42 families sampled in six 96-well plates was carried out at the Service de Systématique Moléculaire (SSM) at the MNHN in Paris using Macherey-Nagel NucleoSpin® 96 tissue kit following manufacturers protocol using either a semi-automated procedure with an Eppendorf Liquid Handling Workstation epMotion® 7075 VAC or a manual approach through successive centrifugations 20 primer tags of 5 nucleotides were re-designed to remain unique after two potential nucleotide degenerations containing all four nucleotides without more than two repetitions and avoiding more than 3 identical successive nucleotides once added to the 5′ end of our primers These primer tags were split in 2 sets of 10 each: AGTCT Tagged-primers were synthetized in NGS grade with HPLC purification by Eurofins Genomics Details of the different COI amplicons targeted in the present study and their associated lengths and overlaps as well as the primers and sequencing technologies used for each fragment the dual-tagging process and ligation of the Illumina indices are represented PCR reactions were conducted separately in two plates (one for each amplicon) in 25 μL with 2.5 μL of 10X CoralLoad PCR buffer 0.5 μL of DNA Taq Polymerase (5 U/μL) from Qiagen 2 μL of DNA template and the final 17.5 μL in extra pure water PCR started with initial denaturation at 95°C for 5 min and final elongation at 72°C for 5 min Approximately 500 ng of DNA were used with 5 μL of NEBNext Repair Reaction Buffer (10X) and 2.5 μL of NEBNext End Repair Enzyme Mix Additional extra pure water was added to reach a 50 μL reaction volume and the mix was incubated at 20°C for 30 min A second purification step was carried out with NucleoMag 1X and an elution volume of 20 μL of TET buffer (0.1X) Adapter ligation was therefore performed in 40 μL by adding 10 μL extra pure water 4 μL T' DNA ligase buffer (10X) 1 μL adapter mix (100 μM each) and T4 DNA ligase (5 U/μL) to the eluate which was then incubated at 22°C for 30 min A third purification with NucleoMag 1X was then performed in 20 μL of EBT buffer To assess the success of the library preparation we performed quantification using Qubit® High-Sensitivity kit and controlled products using migration on agarose gel of positive controls The final PCR indexing enrichment was undertaken after different PCR trials to define the best number of cycles for each sample and starting DNA quantity This final step was done in a 25 μL volume reaction comprising 0.5 μL Qiagen Taq (5 U/μL) 0.5 μL of IS4 primer (10 μM) and 50 ng of DNA template as well as 0.5 μL of indexing primer (10 μM) respective to each sample PCR cycle was as follow: 94°C for 3 min and final elongation at 72°C for 10 min Final purification using NucleoMag 0.85X and eluted in 25 μL of EBT buffer was followed by quantification on Qubit® with High-Sensitivity well plate kit The six sample plates analyzed for the present study were processed along with 35 other plates from other projects and while our first indexing procedure (using dual tagged-primers) aims at demultiplexing reads per sample within each plate, the second step (by Illumina indices ligation) allows for demultiplexing reads by plate (Bourlat et al., 2016) The concentrations of the libraries corresponding to each plate were homogenized before pooling to obtain a fair balance of sequencing reads between the plates processed and according to their contents the six plates analyzed represented 5.6% in concentration of our pooled library which was sequenced using a 600 cycles v3 kit (2 × 300 bp paired-end sequencing) on an Illumina MiSeq at the CIRAD-AGAP sequencing platform in Montpellier PCR conditions were 94°C during 5 min followed by 35 cycles of 94°C during 30 s with a final 10 min extension at 72°C PCR products were deposited on 2% agarose gel and only successfully amplified DNA templates where sent for Sanger sequencing on ABI 3730XL sequencer at Eurofins MWG Operon sequencing facilities (Ebersberg Demultiplexing was done using customized workflows in Geneious V11.0.4 (Kearse et al., 2012) Reads were separated by primer tags with a maximum of one mismatch and a minimum of 2 reads per tag Primers were trimmed and reads were aligned together with MUSCLE 3.8.425 using eight iterations The two amplicons B_R and C_F were merged together by De Novo Assembly with four maximum ambiguities and two base pairs gap sizes over the 85 bp overlapping region and the consensus was then saved in separate folders mirroring wells of sample plates for further curation of the sequences we blasted each consensus against all barcode records on BOLD and NCBI Prior morphological identification established by experts in the collection was used to control the blast results to species or to genus level depending on the availability of DNA barcodes for closely related species the potential presence of identical sequences was checked in other samples from the same plate with particular focus on adjacent wells to assess for potential widespread cross-contaminations we also excluded potential pseudogenes by searching for STOP codons or indels and we investigated possible chimeric sequences (from tag-jumping or incorrect amplicon assembly) through independent identification of both B_R and C_F fragments The identification was also critically revised by experts through reexamination of voucher specimens considering the different potential molecular identifications and taking into account existing synonymy biogeography of sister taxa as well as intra- and interspecific genetic distances to establish the genuine consensus When discrimination of this genuine sequence was impossible Sanger electropherograms for both directions and fragments were assembled to form contigs using Geneious V11.0.4 (Kearse et al., 2012) then aligned and visually checked for quality and noise to resolve some of the ambiguities we ensured no pseudogene presence similarly than with HTS sequences and we checked for potential cross-contamination by blasting sequences on BOLD to test similarity with conspecific and congeneric existing records Low quality electropherograms (potentially due to low DNA concentration DNA degradation or contaminants) were discarded Workflow used in this study for sampling preparation The HTS library we constructed for our 521 sampled individuals representing 343 different species in 39 different families produced an average of 173,664 paired-end reads per pooled plates (sd = 50 083; min = 248 324 reads) with a sequencing depth of around 450X per sample We recovered 286 partial or complete DNA barcodes (i.e. 54.9% of all samples) representing 193 species (56.3% of all species analyzed) The consensus sequences produced were of high-quality with very few ambiguous base-calls (<1% N Sequence length varied with the amplification success of both or either one of the two fragments amplified: we recovered 147 full length DNA barcodes (658 bp) as well as 140 and 19 partial DNA barcodes from the C_F (418 bp) and B_R (325 bp) fragments All records (including failed samples) are publicly available in project PSFOR on BOLD, and all sequenced individuals can be found in dataset dx.doi.org/10.5883/DS-NEWCOLEO and in the Table in Supplementary Material 1 Summary of the sequencing results for the two sequencing technologies the correlation test indicates no significant effect of specimen age on the sequencing ratio success (S = 264 rho = −0.6); age of specimens at time of sequencing seems not to influence sequencing success and 11 only with Sanger sequencing after amplification of the full-length DNA barcode fragment These DNA barcodes represent 199 different species (58% of the species processed) of which 103 are new additions to the reference library for French saproxylic beetles; these new sequences also represent 82 new BINS for BOLD Summary of the genetic distances calculated for sequences with length >400 bp on BOLD with Kimura-2 Parameter and BOLD Aligner for the 297 newly sequenced individuals within the 1,920 sequences of the complete DNA barcode dataset combining our newly generated sequences and preexisting conspecific and congeneric records List of newly sequenced species revealing a maximum intra-specific distance >2% using Kimura-2 Parameter with n being the number of individuals (sequence length >400 bp List of newly sequenced species with a minimum inter-specific distance <2% using Kimura-2 Parameter with (n) being the number of individuals (sequence length >400 Our recovery of DNA barcode sequences with Illumina MiSeq is relatively low (55%) though comparable to that reported in Rougerie et al. (2015) using Sanger sequencing and a similar PCR strategy including failure tracking with internal primers (61%). Other studies showed higher sequencing success but used fresh specimens collected specifically for DNA barcoding (Hendrich et al., 2015: 67%; Pentinsaari et al., 2014: 90%) Our recovery rate with HTS is not higher than Rougerie et al. (2015) but the costs are lower our current cost per sample of the Illumina approach we used here—in the molecular facilities at MNHN excluding labor—is 4 € per sample of which we estimate sequencing cost to represent 0.5 € per sample the current cost of bidirectional sequencing using Sanger on a 96-well plate is 4.5 € per sample meaning that the cost per sample would be 8 € if targeting a single amplicon or 12.5 € if targeting two shorter overlapping amplicons as was the case here when processing degraded DNA from collection specimens this methodology can be applied to various taxa from both newly collected samples and collection specimens and allows processing of a large number of samples for a reduced cost Both issues can blur the genuine signal of consensus sequences resulting in a higher frequency of ambiguities our primers were not designed for this purpose preventing us to shed further light on potential infections our study expands the current coverage of the DNA barcode reference library for European saproxylic beetles by adding 297 newly sequenced records representing 199 species in 31 families of which 103 species (82 new BINs) are new additions to the Barcode of Life Datasystems 26 of which represent genera yet unrepresented in the libraries This generated DNA barcode dataset of well-curated and identified collection specimens will be helpful for fast and reliable taxonomical identification for potential mass-trapping and broad biomonitoring studies using genetic approaches. Saproxylic beetles are of major interest with respect to forest health concerns and the need for identification at species level is of great importance to link functional traits and ecological patterns (Gossner et al., 2013) In total, adding these new sequences to the PASSIFOR dataset (Rougerie et al., 2015) (656 barcodes of 410 species), DNA barcodes reference library now covers 22.4% (598 species out of 2,663 species) of the French fauna of saproxylic beetles (Bouget et al., 2019) When considering records available in BOLD from other European countries We created a checklist in BOLD that can be used both for taxonomical curation and tracking of the completeness of the reference library for French saproxylic beetle's fauna the completeness of the DNA barcode reference library for the French saproxylic beetle fauna is of 57.6% Our results emphasize the interest and potential of using HTS technologies—here Illumina MiSeq—as a fast and affordable approach to barcode collection specimens that may be challenging or costly to process despite a relatively low sequencing success allowed to recover good quality sequences from collection specimens at a reasonable cost All datasets generated for this study are included in the article/Supplementary Material LS carried out the sampling in natural history collections with the help of TN and RR carried out the wet-lab experiments and GP helped in taxonomic identifications LS carried out the analyses and wrote the first draft All authors provided critical feedbacks on the manuscript Experiments were funded both by ANR—Belmont Forum to CLIMTREE project: ANR-15-MASC-0002 (PI: CL-V) and by ANR to project SPHINX: anr-16-ce02-0011-05 (PI: RR) The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest Herniou for helpful comments on the manuscript We are thankful to the three reviewers and to the editor Prof Honeycutt for their helpful comments on the manuscript Laboratory work was carried out at the Service de Systématique Moléculaire (SSM) part of the Service Unit Acquisition et Analyse de Données pour l'Histoire Naturelle (2AD) (UMS2700) at the Muséum national d'Histoire naturelle in Paris The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fevo.2019.00495/full#supplementary-material The devil is in the detail: metabarcoding of arthropods provides a sensitive measure of biodiversity response to forest stand composition compared with surrogate measures of biodiversity “Galaxy: a web-based genome analysis tool for experimentalists,” in Current Protocols in Molecular Biology Les Coléoptères Saproxyliques de France: Catalogue Écologique Illustré Paris: Muséum National D'Histoire Naturelle Google Scholar “Preparation of amplicon libraries for metabarcoding of Marine eukrayotes using illumina miseq: the dual-PCR method,” in Methods in Molecular Biology NY: Springer Science+Business Media) DNA barcoding in forensic entomology - establishing a DNA reference library of potentially forensic relevant arthropod species High-throughput sequencing of multiple amplicons for barcoding and integrative taxonomy Environmental DNA metabarcoding: transforming how we survey animal and plant communities Dufresne, Y. (2017). Double Tagged Amplicon Demultiplexing, GitHub Repository. Available online at: https://github.com/yoann-dufresne/DoubleTagDemultiplexer Google Scholar Can DNA-Based ecosystem assessments quantify species abundance Testing primer bias and biomass—sequence relationships with an innovative metabarcoding protocol An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the illumina MiSeq platform Fagan-jeffries DNA barcoding of microgastrine parasitoid wasps (Hymenoptera: Braconidae) using high-throughput methods more than doubles the number of species known for Australia DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates and the “Taxonomic impediment” CrossRef Full Text | Google Scholar Current near-to-nature forest management effects on functional trait composition of saproxylic beetles in beech forests: functional diversity of beetles The taxonomic impediment in orthopteran research and conservation CrossRef Full Text | Google Scholar A minimalist barcode can identify a specimen whose DNA is degraded More than 75 percent decline over 27 years in total flying insect biomass in protected areas Calibrating the taxonomy of a megadiverse insect family: 3000 DNA barcodes from geometrid type specimens (Lepidoptera A sequel to sanger: amplicon sequencing that scales Biological Identifications through DNA Barcodes A DNA ‘barcode blitz’: rapid digitization and sequencing of a natural history collection Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly astraptes fulgerator Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species A comprehensive DNA barcode database for Central European beetles with a focus on Germany: adding more than 3500 identified species to BOLD Hernández-Triana Recovery of DNA barcodes from blackfly museum specimens (Diptera: Simuliidae) using primer sets that target a variety of sequence lengths Forest continuity acts congruently with stand maturity in structuring the functional composition of saproxylic beetles verifiable and efficient monitoring of biodiversity via metabarcoding Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences PCR with degenerate primers 9 containing deoxyinosine fails with PFU DNA polymerase CrossRef Full Text | Google Scholar Illumina midi-barcodes: quality proof and applications “Preparation of amplicon libraries for metabarcoding of marine eukaryotes using illumina miseq: the adapter ligation method,” in Marine Genomics CrossRef Full Text | Google Scholar Comparison of next-generation sequencing systems $1 DNA barcodes for reconstructing complex phenomes and finding rare species in specimen-rich samples CrossRef Full Text | Google Scholar Illumina sequencing library preparation for highly multiplexed target capture and sequencing CrossRef Full Text | Google Scholar A high-coverage genome sequence from an archaic Denisovan individual Species-level para- and polyphyly in DNA barcode gene trees: strong operational bias in European Lepidoptera A DNA metabarcoding approach to characterize soil arthropod communities CrossRef Full Text | Google Scholar Barcoding beetles: a regional survey of 1872 species reveals high identification success and unusually deep interspecific divergences Universal and blocking primer mismatches limit the use of high-throughput dna sequencing for the quantitative metabarcoding of arthropods Automated high throughput animal co1 metabarcode classification DNA barcodes from century-old type specimens using next-generation sequencing R Core Team (2017). R: A Language and Environment for Statistical Computing (version 3.4.3). Vienna: R Foundation for Statistical Computing. Available online at: https://www.R-project.org/ Google Scholar CrossRef Full Text | Google Scholar A DNA-based registry for all animal species: the Barcode Index Number (BIN) system PASSIFOR: a reference library of DNA barcodes for french saproxylic beetles (Insecta The neighbor-joining method: a new method for reconstructing phylogenetic trees PubMed Abstract | Google Scholar Tag jumps illuminated - reducing sequence-to-sample misidentifications in metabarcoding studies Next-generation DNA barcoding: using next-generation sequencing to enhance and accelerate dna barcode capture from single specimens Massively parallel multiplex DNA sequencing for specimen identification using an illumina miseq platform Wolbachia and DNA barcoding insects: patterns and rapid biodiversity assessment using ants of Mauritius Cambridge; Edimburgh: Cambridge University Press CrossRef Full Text | Google Scholar New approaches narrow global species estimates for beetles Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in human and chimpanzees Google Scholar Mitochondrial phylogenomics of the hymenoptera Chorologie du complexe spécifique Protaetia (Potosia) Cuprea Fabricius Google Scholar Environmental DNA metabarcoding of wild flowers reveals diverse communities of terrestrial arthropods Ten years of next-generation sequencing technology Recovering full DNA barcodes from natural history collections of tephritid fruitflies (Tephritidae Vondráček Phylogeography and DNA-based species delimitation provide insight into the taxonomy of the polymorphic rose chafer protaetia (Potosia) cuprea species complex (Coleoptera: Scarabaeidae: Cetoniinae) in the Western Palearctic Sorting specimen-rich invertebrate samples with cost-effective ngs barcodes: validating a reverse workflow for specimen processing Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring: biodiversity soup An optimised protocol for barcoding museum collections of decapod crustaceans: a case-study for a 10-40-years-old collection Lopez-Vaamonde C and Rougerie R (2019) The Challenge of DNA Barcoding Saproxylic Beetles in Natural History Collections—Exploring the Potential of Parallel Multiplex Sequencing With Illumina MiSeq Received: 01 July 2019; Accepted: 03 December 2019; Published: 19 December 2019 Copyright © 2019 Sire, Gey, Debruyne, Noblecourt, Soldati, Barnouin, Parmain, Bouget, Lopez-Vaamonde and Rougerie. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) distribution or reproduction in other forums is permitted provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited in accordance with accepted academic practice distribution or reproduction is permitted which does not comply with these terms *Correspondence: Lucas Sire, bHVjYXMuc2lyZUB1bml2LXRvdXJzLmZy Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher 94% of researchers rate our articles as excellent or goodLearn more about the work of our research integrity team to safeguard the quality of each article we publish This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page Cabinet Secretaries have announced a series of appointments across various ministries as part of the broader scheme to enhance government operations According to a gazette notice published on Friday Education CS Julius Ogamba appointed Sally Toroitich as a member of the University of Embu Council for one year and two months Ogamba also appointed Parmain ole Narikae and Carren Kerubo as members of the University of Eldoret and Karatina University Councils from April 11 this year to May 22 next year the education minister appointed Asteri Angolo to be a member of the National Biosafety Authority for three years from April 11 Ogamba announced the fresh appointments moments after he revoked the appointment of four University of Nairobi (UoN) Council members Foreign Affairs CS Musalia Mudavadi announced the appointment of Eustus Mureithi Maina and Raymond Komen as members of the Kenya Cultural Centre Council for one and a half years beginning Friday CS Hassan Joho appointed Salim Mohamed as a member of the Mineral Rights Board for three years Agriculture CS Muathi Kagwe also made two notable appointments naming Raymond Komen and Juliet Ngetich as members of the Board of Directors of the National Cereals and Produce Board Energy and Petroleum CS Opiyo Wandayi appointed Beatrice Soy as a member of the Geothermal Development Company Limited Board for three years While announcing Soy's appointment, the energy minister revoked the initial appointment of Eustus Mureithi Maina Lands CS Alice Wahome appointed Jackline Lemaron and Matthew Mpayeei as members of the Land Control Board for the Kajiado West Land Control Area Others who were also appointed to the same Board by the Lands Cabinet Secretary are Moses ole Mamaa (L to R) Paul Njaga CEO Chase Bank Dr Lennie Bazira Ag CEO Amref Health Africa and Parmain Ole Nkaire Chairman of the Chase Group Foundation at the sidelines of the 2015 Chase Group Foundation “Save a Mum” charity walk launch/CFM BUSINESS The walk will be held at the Ngong Road Forest Sanctuary and is expected to bring together over 5,000 participants The walk is in support of the Stand Up for African Mothers initiative by AMREF which seeks to train 15,000 midwives by 2015 therefore reducing maternal mortality by improving access to reproductive health services The walk seeks to raise money towards this noble initiative and train sufficient midwives to adequately tend to the mothers in rural Kenya and Africa as a whole The initiative has garnered monetary support from corporates and individuals alike who have shown great interest in The Foundations dedication to reduce maternal mortality through the training of midwives there have been contributions in kind in the form of water and soft drinks cleaning services and medical services for the walk the Stand Up For Mothers Initiative has trained over 650 midwives in Kenya and 4,000 in Africa Most of the midwives that have been trained have been attached to a health institution in various counties a midwife is able to look after 500 mothers every year and safely deliver 100 babies Statistics show that approximately 1.5 million African children are left motherless each year while over 40 percent of African women do not receive pre-natal care and more than half of all deliveries take place at home without medical assistance This support will provide women of rural Kenya with pre and post natal care a necessity that has previously not been available to them in nurturing their health we are nurturing the future of not only the country but the continent as a whole 162,000 women die every year during childbirth due to lack of simple medical care this walk is one of the many ways we can ensure that this is a thing of the past,” Parmain ole Narikae-Chase Bank General Manager and Chase Group Foundation Chairman stated Amref Health Africa Interim CEO Lennie Bazira Kyomuhangi-Igbodipe said: “Amref Health Africa is deeply concerned about the welfare of women and children particularly those who live in hard to reach parts of Kenya and the African continent with limited or no access to proper and quality health care services It is sad when a mother bleeds to death while giving birth just because there was no one with proper skills to save her she or he has to live without a mother.” The Chase Group Foundation entered into a three-year partnership with AMREF to support the Stand Up For African Mothers Initiative In order to gain public awareness of this cause The Chase Group Foundation hosted the first fundraising walk in February of 2013 Education Cabinet Secretary Julius Ogamba has revoked the appointment of four members of the Council of the University of Nairobi (UoN) In a gazette notice dated April 11, 2025, Ogamba revoked the appointment of Sally Ng’eringwony Toroitich “In exercise of the powers conferred by section 36 (1) (d) of the Universities Act as read together with section 51 (1) of the Interpretation and General Provisions Act the Cabinet Secretary for Education revokes the appointment of Sally Ng’eringwony Toroitich and Carren Kerubo Omwenga as members of the Council of the University of Nairobi This comes barely two months after Ogamba revoked the appointment of Professor Amukowa Anangwe as the chairperson of the UoN council Ogamba sanctioned the decision via a gazette notice published on Friday “In exercise of the powers conferred by Section 36 (1) (a) of the Universities Act as read together with Section 51 (1) of the Interpretation and General Provisions Act the Cabinet Secretary for Education revokes the appointment of Amukowa Anangwe (Prof.) as Chairperson of the Council of the University of Nairobi The decision to fire the chair was preceded by the calls for Amukowa’s resignation The University Academic Staff Union (UASU) wanted the don ejected from the UoN’s leadership citing his alleged mismanagement of the tertiary institution The High Court later overturned Ogamba’s decision to fire Anangwe In the ruling delivered by Justice Bahati Mwamuye of the High Court on Wednesday the court ordered the immediate reinstatement of Anangwe as chairperson of the UON council and barred Ogamba and the Attorney General from appointing a replacement Anangwe had moved to court seeking orders suspending the decision of Ogamba to fire him from the university’s council following complaints that had been raised by the University Academic and Staff Union (UASU) how about joining our vibrant Telegram and WhatsApp channels for hotter stories Stay informed on the latest news by subscribing to the best categories of your interest The Education CS did not immediately announce the replacements of the affected council members Tenures of four members of the Council of the University of Nairobi (UoN) have been ended by Education Cabinet Secretary Julius Ogamba The CS published a gazette notice in which he sanctioned the revocation of the individuals' memberships in the council The affected quartet includes Sally Ngeringwony Toroitich Ogamba did not immediately announce the replacements "In exercise of the powers conferred by section 36 (1) (d) of the Universities Act *revokes the appointment of Sally Ngeringwony Toroitich as Members of the Council of the University of Nairobi which oversees the critical affairs of the university has in the recent past been experiencing routine turnovers CS Ogamba revoked the appointment of Professor Amukowa Anangwe as the chairperson of the council Anangwe's tenure ended immediately after the notice CS Ogamba's decision was preceded by the calls for Amukowa's resignation UASU members threatened a strike if Anangwe would not be removed from the post Previous Post Next Post ODM Slams Standard Newspaper Over Headline Involving Raila’s Family 6 Counties Where Kenya Power is Set to Install Electric Vehicle Chargers Before Saying ‘Ruto Must Go’, First Come Up With Policies - Ruto Hits Out at Critics Postmortem Reveals Where Killer Shot Kasipul MP Charles Were Tanzania Bans Use of Kenyan Shilling and Other Currencies in New Policy