A 67-year-old Georgian-Polish national arrested for espionage in the northern Greek city of Serres was cleared of all charges on Saturday after a court found insufficient evidence of suspicious activity. The defendant, who appears to have been in Greece visiting family, was arrested on Friday morning for taking photographs near the Kleisaris military base, on the outskirts of Serres. He told a judge on Saturday he did not know it was a military facility. His arrest comes after that of another Georgian man who was remanded in custody last week and is due to stand trial for allegedly spying on a NATO base in Alexandroupoli, also in northern Greece, for at least six months. Enter your information below to receive our weekly newsletters with the latest insights, opinion pieces and current events straight to your inbox. Menu.page-389734216{--slot4:#0e111c;--slot5:#ffffff;--slot7:#0e111c;--slot11:#0e111c;--slot13:#0e111c;--slot14:#0e111c;--metaColor:#0e111c;--navColor:#0e111c}(Celebrity) a crystal-covered vintage Dolce & Gabbana dress Courtesy of the French designer’s Fall 2025 collection Bieber stunned in the famous upcycled watch dress which instantly sent the fashion corner of the internet into a frenzy her style streak will likely continue until the F1 race on Sunday stay tuned to TZR for details on her next look It’ll be difficult to top her watch dress (her IG post already has one million likes) 2024 /PRNewswire/ -- Serres has been granted a substantial research grant of 1.8 million from Business Finland Serres is a forerunner in designing and manufacturing surgical fluid management solutions for hospitals worldwide The grant supports Serres´s strategy to provide sustainable solutions that support hospitals in their journey to net zero "The project supports the company's strategic renewal positioning it as a leader in sustainable development by providing solutions for collecting hospital patient fluids and plastic waste This highly ambitious research project combines various aspects within its work packages to achieve the desired results Business Finland's funding allows for implementing a large-scale project that improves international business prospects" states Eija-Riitta Hämäläinen at Business Finland "We are profoundly aware of our responsibility to assist hospitals in their transition to net-zero emissions we also recognize the increasing demand for more sustainable solutions in the healthcare sector and in our field of work" With this grant we can accelerate our undertakings in finding solutions for the future where sustainability and clinical excellence go hand-in-hand Serres' innovation program approaches sustainability on multiple fronts and co-designing new solutions with hospitals Our foremost goal is to ensure responsibility and sustainability within the entire value chain says Maria Lavonen Director of Product group and innovation This grant ensures a comprehensive approach to circular economy in our field and thus the ability to build capabilities and competencies that support our growth in years to come Photo - https://mma.prnewswire.com/media/2550262/Serres_Oy.jpgLogo - https://mma.prnewswire.com/media/2550263/Serres_Logo.jpg Do not sell or share my personal information: vanguardannarbor.com/la-serre Leyland “Lee” DeVito is the editor in chief of Detroit Metro Times since 2016. His writing has also been published in CREEM, VICE, In These Times, and New City. He once asked porn star Stormy Daniels to spank him with an issue of Metro Times. She obliged. ESPOO, Finland, Nov. 7, 2024 /PRNewswire/ -- Serres has been granted a substantial research grant of 1.8 million from Business Finland. Serres is a forerunner in designing and manufacturing surgical Beneath all that entertainingly episodic noise was a constant baseline that took us back to Serre’s mission to refine. The waisted leather looks that opened through to a single-fastening Le Smoking via boxy black tailoring (layered over tiger stripe base layers for a little of the designer’s trademark irreverence) were efforts to apply the brand’s non-conformist philosophy to a more conventionally powerful wardrobe aesthetic. Vivienne Westwood Fall 2025 Ready-to-Wear Maison Rabih Kayrouz Fall 2025 Ready-to-Wear Please select what you would like included for printing: Copy the text below and then paste that into your favorite email application This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply Service map data © OpenStreetMap contributors 2025 ‘Code & State’ secures $3M to incubate stablecoin ventures backed by Warburg Serres and KR1 Press Release COINTELEGRAPH IN YOUR SOCIAL FEED a Dubai-based venture studio specializing in stablecoin innovation has successfully raised $3 million to support the next wave of stablecoin startups will be used to incubate projects focused on expanding real-world adoption of stablecoins both known for their early-stage investments in crypto and Web3 bring deep expertise and strategic networks to Code & State’s mission Their backing reinforces the growing belief that stablecoins will play a pivotal role in global finance over the next decade At the helm of Code & State is Cédric Waldburger a founding investor in Liquity (LUSD stablecoin) and a serial entrepreneur with deep expertise in the crypto space He sees stablecoins as a transformative force in global finance "We believe that the total market cap of stablecoins will 10x in the next five years to over 1.5 trillion dollars," said Waldburger emphasizing the sector's immense growth potential former COO at Dfinity (Internet Computer Protocol) and Co-Founder at Code & State who brings years of experience in scaling Web3 ventures He highlights the unique advantage of the firm’s niche approach: "Our clear focus on stablecoin ventures creates network effects for all our in-house ventures." and infrastructure to startups solving key challenges in the stablecoin space from payment systems and remittances to compliance and security The stablecoin sector is experiencing explosive growth with global transaction volumes exceeding $15 trillion in 2024 The rising adoption of stablecoins for cross-border payments and decentralized finance (DeFi) underscores the need for robust infrastructure and innovation in the space Cédric Waldburger understands the importance of equity incentives for entrepreneurs "It's important to me that our portfolio founders retain 80% of the upside in their venture - we believe it's key to attracting top talent," he said reinforcing Code & State’s commitment to empowering builders Code & State is now actively seeking entrepreneurs-in-residence (EIRs) to develop new stablecoin-focused ventures within the studio Selected founders will receive hands-on support and access to a network of leading investors and industry experts The program is designed to fast-track innovative projects from concept to market-ready solutions ensuring that new startups have the best possible chance of success Application details can be found on the Code & State website Code & State is positioned to be a key catalyst for innovation With a focused investment strategy and strong industry backing the studio is shaping the next generation of stablecoin ventures that will drive mass adoption and unlock new financial opportunities worldwide For more information or to apply for the EIR program This publication is provided by the client Cointelegraph does not endorse and is not responsible for or liable for any content Readers should do their own research before taking any actions related to the company for any damage or loss caused or alleged to be caused by or in connection with the use of or reliance on any content or services mentioned in the press release Serres Toundra is the largest producer of cucumbers in Quebec and offers a superior quality vegetable through environmentally friendly production Serres Toundra operates in highly technological facilities www.serrestoundra.com   the desire to limit its environmental impact is manifested by following its values in sustainable development the Serres Toundra team began looking for a packaging product to replace its polystyrene trays To find a solution that would meet a series of criteria combining performance and respect for the environment Produced and delivered by a reliable supplier The team first turned to a tray made of molded pulp the packaging proved to be ineffective at withstanding the moisture of the cucumbers Only three days after its arrival at the point of sale leaving the consumer perplexed by the look of the packaging Serres Toundra had no choice but to consider another solution That’s when Cascades came on the scene with its 100% recycled, recyclable and compostable cardboard tray the Cascades cardboard tray retains moisture perfectly preserving its rigidity and the integrity of the cucumber for more than 2 weeks Another good news is that this solution generates significant savings in the long run thanks to the elimination of losses during the assembly process “After a week of testing with the Cascades tray It’s hard to say that cardboard packaging alone explains our sales growth but I’m sure it allows us to stand out from our competitors and be favored by retailers.” The collaborative spirit and agile practices of the two companies made it possible to adjust the producer’s assembly line in less than four days while minimizing the financial impact of this innovative transition “The Cascades team deploys its best specialists to optimize operations and reduce the impact of securing a new packaging solution That’s our way of doing things,” says Jérôme Porlier Serres Toundra environmental concerns are shared by consumers more than ever consumers confirms that 55% of them are extremely or very concerned about the environmental impact of product packaging.1 It’s not surprising that Serres Toundra customers appreciate that the packaging is made of 100% recycled fibres and is recyclable/compostable consumer surveys conducted by Cascades show that cardboard is perceived as more environmentally responsible than any other food packaging material Sustainability in packaging: Inside the minds of US consumers Cascades sustainability experts analyzed the packaging choices of Serres Toundra the Cascades cardboard tray allowed Serres Toundra to: If you want to understand the environmental impact of your packaging contact a Cascades expert in sustainable development: Based on the Life Cycle Assessment of the Cascades product (Ageco Group)3 Based on calculations from the Environmental Protection Agency La fraîcheur des poissons et fruits de mer se préserve durant le transport grâce à un isolant étanche et innovant. La solution idéale de remplacement écoresponsable aux emballages non recyclables Visitez la page dédiée à la Northbox ® OCEANMC Packaging Solutions Hygiene Solutions and Tissue Fibre-Based Materials Our Services Recycling Solutions Be part of a responsible and people-centred company.   Sustainable Development Multimedia Library Above the Fold The eco-futurist label's fall 2025 Paris showcase was a trompe-l'oeil-filled comment on money and power inspired by the late filmmaker David Lynch By subscribing, you agree to our Terms of Use and Privacy Policy So, who really killed Laura Palmer? Maybe it was fashion’s excess. For the Fall/Winter 2025 runway, Paris-based sustainable designer Marine Serre paid tribute to the great late director David Lynch Models emerged from claustrophobic blood red curtains and walked across a checkered floor a clear recreation of Twin Peaks’ famous red room each piece was filled with subtle subversions the brand has established itself as a pillar in the conversation surrounding sustainable fashion and environmental protection Marine Serre uses upcycling to deconstruct and rebuild clothing’s identities Serres, an Espoo, Finland-based surgical solutions company, received a €1.8M research grant from Business Finland. The grant supports the company’s strategy to provide sustainable solutions that support hospitals in their journey to net zero. Led by CEO Nicke Svanvik, Serres is developing smart and sustainable surgical fluid management solutions providing program approaches sustainability on multiple fronts, partnering with research institutions, participating in relevant ecosystems, and co-designing new solutions with hospitals. Home The announcement came on Wednesday, shedding light on a growing scandal that has rocked the country’s cultural heritage sector allegedly provided insider information to the looters advising them on prime locations for their illicit digs In a statement released by the Ministry of Culture, it was confirmed that Culture Minister Lina Mendoni acted swiftly upon receiving the police report “By decision of Culture Minister Lina Mendoni [the Guard] is immediately suspended from duties in accordance with the Civil Employees Code,” the ministry declared noting that the case will now proceed under strict regulatory measures The suspension follows a wave of arrests in northern Greece earlier this week, targeting members of the two criminal rings accused of plundering the nation’s archaeological treasures has raised concerns about the security of Greece’s cultural sites and the potential involvement of insiders in such illegal activities the incident underscores the challenges faced in protecting the country’s rich historical legacy The Ministry of Culture has vowed to take all necessary steps to safeguard Greece’s antiquities as the case continues to unfold You must be logged in to post a comment The incident occurred following a dispute in a nightclub in Serres Authorities have arrested two suspects in connection with the murder: a 27-year-old Greek and a 29-year-old Albanian. Two additional suspects, Greek men aged 30 and 28 A case has been filed against the four individuals on charges of manslaughter with intent and conspiracy The tragedy began when the victim attempted to enter a nightclub with two friends A confrontation ensued with one of the suspects but tensions reignited about an hour later The victim and the suspects reportedly crossed paths again in an alley near the city’s main pedestrian street He inflicted two fatal stab wounds on the victim another member of the assailant’s group reportedly discharged a gas-percussion pistol six times in the air Special police analyst Stavros Balaskas “The victim had moved to another pub for a drink when he spotted the bouncer Police responding to the scene discovered six spent shells from the gas-percussion pistol This senseless act of aggression has left a family shattered with a pregnant wife now facing the daunting task of raising three children alone 2025Marine Serre challenges fashion conventions with thought-provoking FW25 "HEADS OR TAILS" collection at Paris Fashion Week ByJenifer Nelly Marine Serreonce again proved her visionary approach to fashion with the unveiling of herFall-Winter 2025collection Hosted at the historic Monnaie de Paris - the French mint - the show was a thought-provoking exploration of duality reinforcing Serre's commitment to reshaping the fashion landscape Serre embraced the concept of chance and duality Guests were presented with a small red box containing a custom minted coin engraved with Serre's signature crescent moon emblem and her own portrait - an artistic statement on identity Serre expressed her motivation behind choosing the Paris mint as her venue emphasizing the challenges young designers face initially pursued a career in tennis before shifting her focus to fashion She honed her craft at prestigious design houses includingAlexander McQueen,Maison Margiela,Dior she became the youngest recipient of the LVMH Prize a defining moment that pushed her to international acclaim redefining the industry with her signature "eco-futurist" philosophy The collection's opening look established the mood - a sleek and iconic catsuit one of the first modern heroines of cinema history as well as the female cat burglars from the hit French TV series "Cat's Eyes." Serre also incorporated references to David Lynch'sTwin Peaks Structured black tailoring and leather took center stage with intricate pieces crafted from antique watches and coins This interplay of radical innovation and timeless elegance reaffirmed Serre's status as a designer unafraid to challenge convention while staying true to expert craftsmanship The show also introduced two new accessories: the MS CRUSH heels and the AURORA bag adding another layer of desirability to the collection Despite the industry's commercial pressures I have to have two brains because I'm also the owner of the company But I think it's important not to be driven by that in a creative position," she stated "I want to really make garments that people can keep for life With "HEADS OR TAILS," Marine Serre masterfully combined sustainability with storytelling proving once again that she is one of the most thought-provoking designers of her generation and ethical responsibility ensures that her influence in fashion will continue to grow Celebrity guests in attendance included:Bella Thorne,Kalash,Christina Kirkman,Mei Pang,Heyoon Jeong,AsakeandEva Apio. Rain Magazine stands at the forefront of fashion, interweaving the dynamic worlds of music, culture, and art. is also an agricultural and cotton farming base The Land Improvement Organization of Psychiko-Pethelino (TOEB) through a public tender performed by the Municipality of Emmanouil Pappas selected ABB to upgrade the irrigation pumping stations in the area to optimize the energy used for the water irrigation infrastructure which acts as the backbone of local farming activities “We have seen ABB’s work in the irrigation and wider water industry which encouraged us to use ABB’s automation and digital solutions for our pumping stations and modernize the entire irrigation infrastructure.” said Chr Head of Technical Department at Emmanuel Pappas municipality With decades of experience supporting the water and irrigation industry worldwide ABB is one the leading providers of automation and digital solutions enabling improved performance while driving energy efficiency “Winning this project has assured us of the trust our customers have in us.” said Michalis Kanellopoulos Sales Manager ABB Energy Industries in Greece “Our solutions provide a holistic approach to maintaining a higher level of operational excellence The success of this project enhances ABB’s position in the water and irrigation infrastructural solutions industry.” “Our solutions will help establish a robust irrigation system with the best energy performance that will support the essential work performed by the local farmers.” said John Kokkotos Local Division Manager ABB Energy Industries in Greece ABB received the contract from TOEB Psychiko-Pethelino in March 2024 and has been working with the local public company to understand the irrigation system gaps in the pumping stations ABB will install a distributed control system programmable logic controllers and variable speed drives to secure an energy-efficient flow of water By installing ABB’s intelligent data-driven digital solutions and systems TOEB Psychiko-Pethelino aims to improve the irrigation and water infrastructure leading to greater energy efficiency and reduced energy consumption “Our solutions aim to help achieve a reduction of almost 15 percent in energy consumption once the project has been completed.” said Michalis Kanellopoulos Sales Manager Energy Industries ABB Greece ABB will provide remote control capabilities to the upgraded pumping stations enabling operators to monitor operations and troubleshoot whenever any issue arises ABB's website uses cookies. By staying here you are agreeing to our use of cookies. Learn more I agree the second child of Clyde and Mary Lou (Kohne) Roed She grew up in the Piedmont Heights neighborhood and graduated from Duluth Cathedral High School in 1966 where she first met and started dating John Serre Nancy proudly attended the University of Minnesota-Duluth where she earned a Bachelor of Arts degree in Sociology along with a minor in Psychology in 1970 Nancy was a member of both Holy Rosary Catholic Church and St Nancy dedicated more than 40 years to social work for St including work as a Child Protection Specialist team member of initial intake child abuse investigations and countless years as a foster care recruiter Nancy helped thousands of at-risk Women and Children in the Northland to find safety She also volunteered much of her time as a Guardian ad Litem advocating for the best interests of children in juvenile protection cases for Minnesota’s 6th Judicial District Nancy had a passion for volunteering with years of dedication to Birthright International She lived her life to help those unable to help themselves.  in Duluth and together they raised three sons Their greatest happiness has always been their son's achievements Nancy is survived by her loving husband of 54 years John; their sons Matt (Amy); Nic (Mary); and Jordan (Cami); her sisters Mary (Jerry) Maurer; Barb (Ted) Lammi; and Lynne (Steve) Sexton; their grandchildren Peter Nancy will forever be remembered as a loving Wife Nancy donated her body to the University of Minnesota’s Anatomy Bequest Program to support medical research through education for health professionals and continued advancements in medical care A Mass of Christian Burial  will be held on Thursday 2024 at 10:00 am with a visitation starting at 9:00 am at St In lieu of flowers, the family asks for donations in Nancy’s memory be made to Birthright International; https://www.mightycause.com/donate/Birthright-Usa-National as she gave so much of her time to the organization over the years.  Enter your phone number above to have directions sent via text CA) — Serres Ranch expects to begin harvest on their blueberry farm in Laytonville California today with blueberries slotted to be available in local grocery stores and featured on menus as early as next week “We have a great local following for our sustainably-grown blueberries.” Taylor Serres-Murnig a fifth-generation member of the Serres farming family says “Blueberry harvest is hard work but it pays off in seeing the excitement over the annual crop and our great restaurant catering and bakery partners who use the fruit to make unique and delicious creations each year.” The Serres family has been farming blueberries on their property in Laytonville for 23 years and has gained a local following for the berries which are available for about a month each year from June to July The family attributes the popularity to the quality which are grown using no sprays or pesticides and which make their way from the bush to the market in less than 48 hours.  The larger-than-average size of the berries is due to the blueberry varietal: Duke Northern High Bush which thrives in cooler climates and is known for large berries with a delicate sweet and tangy flavor “During the fall the plants went into dormancy and got a good long winter sleep The cooler spring provided favorable weather during the bloom less heat units than we would prefer therefore it delayed ripening ever so slightly The weather currently is mild conducive to plentiful fruit ripening The crop looks good its coming along and ripening nicely and we are excited to get these berries off the bushes and into our customers hands!” says John P Serres Ranch Blueberries can be found from late June to July in Sonoma Market Pacific Market – Sebastopol and Santa Rosa ID and NV and are featured in recipes or products at many local restaurants and operated ranch and vineyard in the heart of the Sonoma Valley the Serres family has worked tirelessly alongside Mother Nature to grow the finest quality grape varietals for some of the most acclaimed and recognized brands in both Napa and Sonoma The legacy continues with the fifth generation of Taylor and Buck overseeing the meticulous attention to detail in farming their estate vineyards and creating delicious wines that honor their rich family history © 2007 - 2025 | Wine Industry Network LLC. All Rights Reserved. A badly burned body found midday on Thursday in a field in Serres is believed to belong to a 59-year-old man who strangled his wife to death earlier in the day the body was found some 500 meters from the house where a 52-year-old woman was found dead on Thursday morning had reportedly called the police to report the crime and then disappeared before officers arrived at the scene He is said to have left a note expressing his intention to kill himself Enter your information below to receive our weekly newsletters with the latest insights opinion pieces and current events straight to your inbox Get an exclusive look at Marine Serre‘s Fall 2025 fashion show from the runways of Paris Fashion Week small businesses stand at the forefront of innovation,” writes columnist Nicole Smartt Serres In an era of rapid technological advancement small businesses are discovering powerful tools that once seemed reserved for corporate giants Today's entrepreneurs are leveraging cutting-edge technologies to compete and thrive in an increasingly digital marketplace Small businesses are embracing cloud technologies as a lifeline to scalability and efficiency Services like Amazon Web Services and Microsoft Azure allow companies to access enterprise-level computing power without massive infrastructure investments A local marketing firm in Santa Rosa can now store and analyze terabytes of data without purchasing expensive servers dramatically reducing operational costs and increasing flexibility and instant scalability that was unimaginable just a decade ago AI has emerged as a game-changer for small businesses offering intelligent solutions across multiple operational domains ChatGPT and similar AI platforms help businesses generate marketing content Local restaurants use AI to create menu descriptions while consulting firms leverage these tools to draft proposals and reports and provides sophisticated capabilities that level the playing field with larger competitors Smart devices are revolutionizing how small businesses operate and monitor their environments Retailers use IoT sensors to track inventory in real-time while manufacturing shops employ connected devices to monitor equipment performance and predict maintenance needs A small bakery can now use smart thermometers to ensure perfect food storage while a local construction company can track equipment location and usage with unprecedented precision and provide data-driven insights previously unavailable to smaller enterprises blockchain is offering small businesses enhanced security and transparency Local suppliers use blockchain to verify product authenticity while service-based businesses implement smart contracts that automate payment processes A small agricultural business can now track produce from farm to table providing customers with complete transparency about product origins and creates trust in business transactions Small businesses are discovering innovative applications for augmented reality that transform customer experiences Furniture stores allow customers to visualize products in their own spaces using AR apps Real estate agents provide virtual property tours while clothing boutiques enable virtual try-on experiences and provide immersive experiences that were once impossible for smaller businesses small businesses are investing in sophisticated cybersecurity technologies and comprehensive protection platforms help companies defend against potential breaches These solutions provide enterprise-level protection at affordable prices allowing even the smallest businesses to safeguard critical data and maintain customer trust High-speed mobile networks are transforming how small businesses operate and communicate Food trucks can process payments instantly field service technicians can upload real-time data and remote workers can collaborate seamlessly and innovative mobile solutions that expand business capabilities beyond traditional limitations Small businesses are using RPA to automate repetitive tasks reducing human error and increasing efficiency Accounting firms automatically process invoices while customer service departments use chatbots to handle routine inquiries These technologies allow small teams to accomplish more redirecting human talent toward more strategic Processing data closer to its source is revolutionizing how small businesses handle information Retail stores can analyze customer behavior in real-time while manufacturing businesses can monitor equipment performance with minimal latency more efficient data processing that enables more immediate decision-making Small businesses are leveraging data analysis tools to make more informed decisions and marketing teams optimize campaign strategies These technologies transform raw data into actionable insights allowing smaller companies to compete with larger small businesses stand at the forefront of innovation proving that size is no longer a barrier to technological sophistication getting involved with Ducks Unlimited Canada (DUC) was a natural continuation of his lifelong investment in education and dedication to serving his community De Serres worked in health care for 30 years at the Quebec Institute of Public Health and as a professor of epidemiology at Laval University He was named professor emeritus when he retired in 2023 De Serres began taking courses for a bachelor’s degree in biology — returning to a career path he’d set out on decades ago “I actually started in biology when I went to university in 1973 I worked for the Canadian Wildlife Service on the Gaspé Peninsula and Bonaventure Island with a large gannet colony,” he says De Serres was interested in doing volunteer work around greenhouse gas emissions but a conversation with his daughter-in-law piqued his interest in wetlands “She told me a lot of people work on greenhouse gas issues but not a lot of people are focused on wetlands even though they are so important to biodiversity and carbon capture,” says Dr I had been donating to Ducks Unlimited for years and I loved how they used science to protect the wetlands which matched up with my own scientific background Getting the word out about wetland conservation is where Dr. De Serres truly shines he penned over 300 scientific papers and conducted countless lectures — invaluable science communication skills that he’s generously shared with Ducks Unlimited Canada Since joining the organization just over a year ago De Serres has supported Ducks Unlimited Canada as a representative at last year’s Quebec Outdoor Fishing and Camping Show and contributed writing to several digital educational story maps for the Ducks Unlimited Canada website De Serres’ exceptional contributions to Ducks Unlimited Canada and are honoured to name him Volunteer of the Year in Quebec De Serres in the Ducks Unlimited Canada office at least once a week collaborating with his fellow volunteers sharing his knowledge and coming up with new ideas He’s planning on spending even more time with Ducks Unlimited Canada in the coming year “The group we have here is really enthusiastic and positive,” says Dr It’s been a joy to be involved in the movement alongside them.” De Serres has felt a deep sense of fulfillment seeing the impact that Ducks Unlimited Canada has had on the natural areas he cares about “There’s a marsh near my cabin in Saguenay that was restored by Ducks Unlimited there were 10 goslings,” he says with a smile “When you’re part of the organization that’s done the work and you get to see what you’re protecting it gives you a positive example of the change that can happen That’s a big incentive for me to keep working with Ducks.” After years of being of service to others through teaching and medical research De Serres remains committed to staying engaged in important issues and giving back in retirement “I see many people in their retirement who are doing activities only about themselves I want to continue to contribute to the world There are approximately 3,030 Ducks Unlimited Canada volunteers across Canada. Ducks Unlimited Canada is proud to recognize each of them — including leaders like Gaston De Serres — as volunteer champions and conservationists who make waves and together create ripples of change by serving with generosity Join our passionate flock of volunteers and make a difference for wetlands and wildlife in your community Quebec carver’s artistic skills are tied to the beauty of waterfowl and the conservation efforts that sustain them Bettylou Christenson named Ducks Unlimited Canada’s British Columbia Volunteer of the Year Layton Ford named Ducks Unlimited Canada’s New Brunswick Volunteer of the Year and wildlife for the generations of tomorrow Donate Help us improve our website by providing feedback Submit Website Feedback For more information, click here. Marine Serre returned to the runway for the Fall/Winter 2025 season of Paris Fashion Week inviting guests to the La Monnaie de Paris — a historic venue that quite literally produces France’s money A unique juxtaposition for a young and independent designer the location aimed to represent Serre’s resistance “in a world run by financial dictates.” guests sat on seats complete with boxed gold coins stamped with Serre’s profile on one side and the signature crescent moon emblem on the other The collection that followed aimed to represent freedom looking back at the last seven years of the brand has closed permanently ahead of the 2024-2025 ski season as fellow European destinations tangle with ongoing climate change impacts Alpe du Grand Serre’s closure threatens 200 jobs leaving local community members dependent on the resort in a lurch The Guardian reports that the decision stemmed from Alpe du Grand Serre lacking enough funds to become a year-round destination nearly 3 million euros have been funneled into the creation of hiking and biking trails at the resort but local councilors decided that they couldn’t continue paying the high price associated with these improvements The project was two years from completion.Alpe du Grand Serre’s closure threatens 200 jobs “We still have loans to pay as we’ve only been here four years That’s what is going to happen to us,” the owner of a grocery store in nearby La Morte told The Guardian low and mid-altitude resorts throughout Europe have been forced to invest in all-season attractions as snowfall becomes less reliable The Swiss ski area of Skilift Schratten Flühli recently ceased operations citing climate change and financial woes between 53% and 98% could face snow supply challenges without snowmaking The percentage range of impacted resorts decreases to 27% and 71% with snowmaking although an increased reliance on snowmaking poses another challenge: boosted demand for water and electricity the latter of which can contribute to carbon emissions I was captivated by the elegant style of Repetto’s ballerinas they were manufactured near my home in Saint Médard d'Excideuil visiting the factory was a magical occasion as it introduced us to the world of artisanal craftsmanship,” Serre said in a press release adding that she was delighted to see her signature half-moon print reproduced on these iconic shoes.The half-moon used by Serre since her early days in fashion looks as though it is embossed on the front of the Lilouh ballerinas Marine Serre’s famous half-moon pattern is also featured on the Camille ballerinas as opposed to the flat sole of the iconic Cendrillon model black and white patent leather and in denim fabric His Beatitude the Patriarch of Jerusalem Theophilos received the Most Reverend Metropolitan Theologos of Serres who led a 50-member group of pilgrims from his God-saved Metropolis alongside a small group of pilgrims from the Holy Metropolis of Thebes and Livadia underlining the importance of the strength derived from the pilgrimage-vision at the All-holy Shrines which is being unjustly fought against by the current forces The Most Reverend Metropolitan of Serres thanked His Beatitude on behalf of the pilgrims for receiving them and for His words of wisdom and experience in the Church of Zion and the All-holy Shrines to which He has dedicated His life since His early youth His Beatitude thanked the Metropolitan of Serres for his words as well as for the hospitality the camp sites of his Holy Metropolis have offered the youngsters of the Patriarchate Congregation to send students to the reorganized School of Holy Zion from his Metropolis ” order_by=”sortorder” order_direction=”ASC” returns=”included” maximum_entity_count=”500″]  Copyright of website material is allowed provided the reference source is mentioned: Jerusalem Patriarchate – Website - www.jerusalem-patriarchate.info The Alpe du Grand Serre ski resort in the French Alps has permanently closed, citing financial difficulties and the impacts of climate change, France3 reports local authorities voted not to reopen the resort for the upcoming winter season effectively ending its 85-year run as a popular winter sports destination will result in the loss of approximately 200 direct and indirect jobs including 25 ski instructors and their families The decision will significantly impact the economy of the nearby village of La Morte which has a population of just 150 residents director of the French Ski School (ESF) at the resort expressed his concern about the sudden closure “It’s a tragedy for the whole valley.” He noted that the ski school works throughout the summer to prepare for winter highlighting the resort’s year-round impact on local employment pointed to mounting annual deficits and insufficient funds to complete an ongoing project to transform Alpe du Grand Serre into a year-round destination Despite investing nearly €3 million since 2021 in diversification efforts such as developing hiking and biking trails the community lacked the resources to sustain the project for its final two years expressed frustration at the lack of government support “The state is not giving us any concrete support for the future of the resort or a transitional operation.” Frédérique Laurence runs a restaurant near the resort and added “We have been left completely in the lurch The Guardian reports that the closure of Alpe du Grand Serre follows a pattern observed by geographer Pierre-Alexandre Metral of Grenoble University. He noted that an estimated 180 smaller French ski resorts have been forced to close since the 1970s due to various factors, including climate change, poor management, and competition from larger resorts. Alpe du Grand Serre is smaller than major French resorts like Val d’Isère or Courchevel, but it’s comparable to other mid-sized resorts in the region. The closest major ski resorts are Alpe d’Huez, about 25 miles (40 km) to the east, and Les Deux Alpes, approximately 31 miles (50 km) to the southeast. The ski and snowboard site with intelligence. Volume 18 - 2024 | https://doi.org/10.3389/fncel.2024.1414142 This article is part of the Research TopicExtracellular vesicles: emerging roles in the aged and neurodegenerative brainView all 5 articles Extracellular vesicles (EVs) are secreted by all cells in the CNS EVs are lipid membrane enclosed particles loaded with various bioactive cargoes reflecting the dynamic activities of cells of origin the specific role of EVs released by astrocytes is less well understood partly due to the difficulty in maintaining primary astrocyte cultures in a quiescent state The aim of this study was to establish a human serum-free astrocyte culture system that maintains primary astrocytes in a quiescent state to study the morphology and protein cargoes of astrocyte-derived EVs Serum-free medium with G5 supplement and serum-supplemented medium with 2% FBS were compared for the culture of commercially available human primary fetal astrocytes Serum-free astrocytes displayed morphologies similar to in vivo astrocytes higher levels of astrocyte markers compared to astrocytes chronically cultured in FBS astrocyte and inflammatory markers in serum-free astrocytes were upregulated 24 h after either acute 2% FBS or cytokine exposure confirming their capacity to become reactive this suggests that distinct signaling pathways are involved in acute and chronic astrocyte reactivity chronically serum-cultured astrocyte-derived EVs (ADEVs) were smaller in size compared to serum-free ADEVs and could reactivate serum-free astrocytes Proteomic analysis identified distinct protein datasets for both types of ADEVs with enrichment of complement and coagulation cascades for chronically serum-cultured astrocyte-derived EVs offering insights into their roles in the CNS these results suggest that human primary astrocytes cultured in serum-free medium bear similarities with in vivo quiescent astrocytes and the addition of serum induces multiple morphological and transcriptional changes that are specific to human reactive astrocytes and their ADEVs more emphasis should be made on using multiple structural and functional parameters when evaluating ADEVs as biomarkers of astrocyte health One of the main challenges with neurodegenerative diseases is the length of time it takes to reach a diagnosis, which means that patients cannot access essential support or begin possible treatments until later in the disease progression. Given that astrocyte reactivity typically emerges in the early stages of disease, astrocyte secretions hold promise as a biomarker source for early diagnosis and disease stratification (Escartin et al., 2019) astrocytes would not be exposed to components of serum in an intact BBB they should not proliferate under healthy conditions Serum should be absent from quiescent astrocyte models yet reliable and reproducible serum-free culture systems for human primary astrocytes are currently unavailable With a simple serum-free method to culture human primary astrocytes it would be therefore possible to mimic the in vivo brain microenvironment as well as offering translational value when evaluating ADEVs as clinical biomarkers of astrocyte reactivity in CNS pathologies we cultured commercially available human primary astrocytes in an optimized serum-free medium using G5 supplement and compared their morphological and transcriptional changes to treatments with either 2% FBS or cytokines using immunocytochemistry ADEVs from both serum and serum-free media were isolated to study their morphology and function and proteomic analysis was used for characterizing protein cargoes in ADEVs Human primary astrocytes (SC1800s) were purchased from ScienCell (Cat# SC-1800; distributed by Caltag Medsystems UK) and stored in liquid nitrogen (−196°C) prior to use cells were cultured according to the supplier's instructions astrocytes were cultured in astrocyte medium (ScienCell 1% astrocyte growth serum (AGS) and 1% penicillin/streptomycin solution (P/S) and maintained at 37°C in a humidified atmosphere of 5% CO2 Cells were seeded at a density of 4 × 103 cells/cm2 and passaged every 3–4 days until a maximum of 7 passages thawed cells from the supplier were initially cultured with serum for a single passage to encourage proliferation before cryopreserving in liquid nitrogen (-196°C) in a solution of 90% FBS (Sigma UK) and 10% dimethyl sulfoxide (DMSO; Sigma cells were spun down and thawed in serum-free medium [advanced DMEM (Gibco UK)] supplemented with 1% G5 supplement (Fisher scientific UK) with a medium change occurring after 24 h to remove any residual serum from the cryopreservation cells were seeded at a high density (1.6 × 104 cells/cm2) and sub-culturing was not required a medium change was performed every 3–4 days Serum-free astrocytes were treated with either 2% FBS (Caltag, UK) or an inflammatory cytokine cocktail for 24 h. The cytokine cocktail was made up of recombinant human IL-1α at 100 μg/mL, TNFα at 50 μg/mL (both Peprotech, UK) and C1q at 400 ng/mL (Merck, UK), which had previously been shown to induce an inflammatory phenotype in astrocytes (Liddelow et al., 2017) To harvest sufficient EVs for downstream approaches at least four collections of medium were completed from serum-free astrocytes (over 2 weeks) and combined for processing conditioned medium was preserved at −80°C until needed FBS needed to be removed from the medium before ADEVs were collected to avoid contamination of bovine EVs cells were seeded as usual in astrocyte medium containing 2% FBS the cells were carefully washed with PBS before the astrocytes were cultured in astrocyte medium without FBS with the reactive phenotype being maintained The conditioned medium was then collected after 72 h and stored at −80°C until needed A minimum of 4x T75 flasks of medium were required to harvest enough EVs for downstream processing The conditioned medium (~48 mL) was concentrated to 500 μL through centrifugation using 10 kDa MWCO protein concentrators (ThermoFisher scientific The concentrated 500 μL was then added to a washed qEV original 70 nm column (IZON Three fractions were collected according to the manufacturer's instructions the column was washed with both PBS and 0.5 M NaOH solution equal to 2 column volumes Fractions were frozen at −80°C for longer term storage when required If a more concentrated EV solution was required for downstream applications EV fractions were combined and centrifuged using 3 kDa MWCO protein concentrators (Merck UK) until the desired volume was required (>10-fold concentration) EV-enriched fractions were fixed in 3% glutaraldehyde and loaded onto copper carbon-coated 200-mesh electron microscopy grids (EM resolutions Grids were then imaged on the Tecnai Spirit Bio-twin T12 transmission electron microscope (TEM) Nanoparticle tracking analysis (NTA) was completed on the Zetaview® (Analytik UK) to measure the size and concentration of the EVs within the EV-enriched fractions The three EV fractions obtained from SEC were combined before measuring EV concentration using NTA EVs were diluted to 2.5 – 5.0 × 1011 particles/mL Serum-free astrocytes were then treated with either 10 μL (2 × 109 particles) or 100 μL (2 × 1010 particles) of purified reactive ADEVs for 24 h Astrocytes were plated onto 13 mm coverslips at a density of 1 × 105 cells/coverslip for serum-cultured or 2 × 105 cell/coverslip for serum-free astrocytes in a 24-well plate and incubated at 37°C and 5% CO2 for 48 h permeabilised for 10 min using 0.1% (v/v) Triton X-100 in PBS and then blocked using 1% (w/v) bovine serum albumin (BSA) for 1 h at room temperature Cells were then labeled overnight with either anti-GFAP (rabbit UK) was applied for 1 h at room temperature the coverslips were then mounted onto glass slides using VectorShield mounting medium containing 4,6-diamidino-2-phenylindole (DAPI; VectorShield UK) and stored at 4°C until imaged Images were taken using an inverted fluorescent microscope equipped with a Camera Axiocam 305 monodigital (Axio Vert A1 Image analysis was then completed using FIJI software (Image J The 16830 most expressed genes were used to run the multivariate statistical analysis To harvest astrocytes for western blotting Cells were scraped and harvested in fresh PBS Cells were pelleted by centrifugation at 1,500 × g for 5 min at 4°C and cells were lysed for 15 min in RIPA lysis buffer (5 M sodium chloride 10% (w/v) SDS) with protease and phosphatase inhibitors at a dilution of 1:1000 Cell debris was pelleted and removed by centrifugation at 16,100 × g for 20 min 4°C The supernatants were collected to calculate protein concentration using a Pierce BCA assay kit (ThermoFisher Scientific Samples of 1–10 μg protein were heated for 5 min with 5 μL gel application buffer (0.15 M Tris then separated using a 5%-20% polyacrylamide gradient SDS-PAGE gel Proteins were transferred overnight to a nitrocellulose membrane (AmershamTM Protran® western blotting membrane UK) in Western Transfer Buffer (25 mM Tris 192 mM glycine and 20% (v/v) methanol) at 40 mA and 500 V The membrane was washed 3x in Tris-buffered saline (TBS; 20mM Tris pH 7.5) with 0.1% (v/v) Tween (TBS-T) before blocking in 5% milk-TBS-T (5% (w/v) Marvel milk powder in TBS-T) for 1 h at room temperature The membrane was then incubated with primary antibody diluted in 5% milk-TBS-T overnight at 4°C Primary antibodies were washed with 3x TBS-T then a secondary peroxidase rabbit anti-mouse antibody (1:3,000 UK) in blocking buffer was incubated with the membrane for 1 h a 1:1 mix of Western Lightning Plus ECL reagents (enhanced chemiluminescence UK) was incubated with the membrane for ~1 min the membrane was developed on film and fixed Western-blot densitometry of the film was then performed using FIJI software (Image J protein extracts were prepared as for immunoblotting the EV fraction with the most particles (i.e. EV fraction 2) was heated with 4x Laemmli sample buffer (Bio-Rad All samples were electrophoresed on a 5%−20% gradient SDS-PAGE gel until the sample had passed through the stacking gel and had entered at least 0.5 cm of the resolving gel maintaining the proteome in a single gel band The entire gel was stained with Coomassie dye (Bio-Rad UK) to highlight the region of the gel to be cut where the protein content for each sample migrated Samples of serum-free and serum-cultured human astrocytes and corresponding EV fractions purified from conditioned media were analyzed independently using LC-MS/MS (Cambridge Center for Proteomics; Cambridge fragment ion mass tolerance 0.100 Da) and validated with Scaffold 5 (Proteome Software Carbamidomethyl (+57 on C) was considered a fixed modification and acetylation (+42 on K/N-terminal M) were considered as variable modifications our criteria were a minimum of two unique peptides within a run with peptide threshold set at 95% and protein threshold 99% giving a mean FDR (false-discovery rate) of ~17% across runs “presence” and “absence,” across biological repeats Recognizing the common issue of complexity and variation (or stochasticity) in sampling between LC-MS/MS runs protein detection in at least 2/3 biological repeats was applied A negative binomial distribution model was used to calculate significance followed by the Benjamini–Hochberg procedure with a differential gene screening threshold of log2 (FoldChange) ≥ 1 and padj ≤ 0.05 this contrasted with when the cells were cryopreserved in liquid nitrogen (−196°C) after initial culture in a solution of 90% FBS and 10% DMSO and then revived in serum-free medium with a quiescent phenotype Serum culture causes a morphological change in human primary astrocytes and promotes cell maturation (A) Representative brightfield images (first row) immunofluorescence images of GFAP (second row) EAAT2 (third row) and S100β (fourth row) showing morphological differences between serum-free (– left column) and serum-cultured astrocytes (+ (B) Graphs showing increased area/perimeter ratio (***p < 0.001 at least 20 cells counted per samples) and (C) nuclear size (*p < 0.05 at least 70 cells counted per samples) for serum-cultured vs E) Immunoblots showing higher GFAP protein levels in serum-free compared to serum-cultured astrocytes (**p < 0.01 and (D–F) similar EAAT2 protein expression in both serum-free and serum-cultured astrocytes Scale bars represent 100 μm for all images Data represent mean ± SEM and analyzed using unpaired t-test Measurements were performed after 1 week in culture While GFAP is well established as an astrocyte marker, these results confirm that quiescent human primary astrocytes showed similarity with in vivo astrocytes, but they do not correlate with increased GFAP expression when astrocytes are chronically cultured in serum, as seen in rodent primary astrocytes (Prah et al., 2019) suggesting that human primary astrocytes cultured in serum display an inflammatory secretory phenotype Transcriptional profiles of astrocyte and inflammatory markers are different in serum-free and serum-cultured astrocytes (A–D) Real-time quantitative PCR analysis of GFAP showing downregulation of astrocyte markers in serum-cultured astrocytes compared to serum-free astrocytes (**p < 0.01; *p < 0.05 vs (E–H) Real-time quantitative PCR analysis of CD49F showing upregulation of inflammatory markers IL-1β and IL-10 in serum-cultured astrocytes (*p < 0.05 vs Data represent mean ± SEM and were analyzed using unpaired t-test RNA-sequencing analysis reveals distinct transcriptomes for serum-free and serum-cultured astrocytes (A) The majority of protein coding RNA was found in both models but more unique RNAs were identified in serum-free astrocytes (B) Principal component analysis (PCA) highlights the differential RNA expression between serum-free and serum-cultured astrocytes (C) The expression of the 20 most influential genes identified in the PLS-DA analysis contributing to the separation between serum-free (SF) and serum-cultured astrocyte (SC) transcriptomes (D) Most RNAs identified were differentially expressed between culture conditions with more RNAs downregulated in serum-cultured astrocytes (red) compared to serum-free astrocytes (E) KEGG pathway analysis showing upregulation of supportive pathways such as axon guidance and synapse associated pathways such as GABAergic synapse and glutamatergic synapse in serum-free astrocytes compared to serum-cultured astrocytes (F) KEGG pathway analysis showing upregulation of CNS disease-associated pathways as well as increased ribosome-related pathways in serum-cultured astrocytes To further test the ability of serum-free astrocytes to become reactive, astrocytes were acutely treated for 24 h with either 2% FBS or cytokines that are secreted by activated neuroinflammatory microglia (i.e., IL-1α, TNFα and C1q) (Liddelow et al., 2017) The area/perimeter ratio was used to assess morphological changes in both untreated and treated serum-free astrocytes The acute effect of serum and cytokines induces morphological changes in serum-free astrocytes (A) Representative bright field images (top row) and immunofluorescence images of GFAP expression (bottom row) showing morphological differences between untreated (left column) 2% FBS treated (middle column) and cytokine-treated (right column) serum-free astrocytes (B) Morphological analysis showing that 2% FBS significantly increases area/perimeter ratio in serum free astrocytes whilst cytokine treatment does not (**p < 0.01 (C) Graph showing the area/perimeter ratio of serum-free astrocytes in response to 2% FBS over 24 h (*p < 0.05 (D) Representative brightfield images of serum-free astrocytes in response to 0 (E) Morphological analysis showing that FBS dose-dependently increases the area/perimeter ratio of serum-free astrocytes (*p < 0.05 ***p < 0.001 and ****p < 0.0001 Data represent mean ± SEM and were analyzed using one-way ANOVA followed by either Tukey's or Dunnett's multiple comparison test Measurements were performed after 24 h exposure to 2% FBS or cytokines Similar changes in the expression of astrocyte (GFAP, S100β) and inflammatory specific genes (IL-1β, TNFα) were evident in the acute response to 0, 2 and 10% FBS (Supplementary Figure 2). However, there was no dose-dependent effect of FBS concentration, as seen in the morphological analysis (Supplementary Figure 2) Transcription profiles of astrocyte and inflammatory markers in serum-free astrocytes are different upon acute exposure to either 2% FBS or inflammatory cytokines (A) Real-time quantitative PCR analysis of GFAP expression showing a significant increase after 2% FBS treatment but not after cytokine treatment (*p < 0.05 vs (B–E) Real-time quantitative PCR analysis of S100β showing a trend toward downregulation of these markers in cytokine-treated astrocytes only S100β expression was significantly downregulated (*p < 0.05 vs No significant difference was identified after acute FBS treatment (F–H) Real-time quantitative PCR analysis of IL-1β showing significant upregulation of inflammatory markers IL-1β and TNF-α in cytokine-treated astrocytes but not in FBS-treated astrocytes (**p < 0.01 vs Data represent mean ± SEM and were analyzed using one-way ANOVA followed by Dunnett's multiple comparison test confirming gene expression analysis seen by RT qPCR These results suggest that acute serum exposure induces morphological changes in astrocytes in a dose-dependent manner acute exposure to serum seems to activate different signaling pathways in astrocytes compared to chronic serum-culture condition as astrocyte and inflammatory markers were expressed differently in these conditions To investigate whether chronic serum culture affects the morphology and protein cargoes of ADEVs we optimized an isolation procedure to effectively collect a large quantity of ADEVs from serum-free astrocytes conditioned medium collection was completed four times from a 6-well dish culture until 48 ml of conditioned medium was collected astrocytes were initially seeded into four T75 flasks with serum-containing medium for 24 h before medium was replaced with astrocyte medium without FBS Cells were then cultured for 72 h before conditioned medium was collected Comparison of serum-free and serum-cultured astrocyte-derived extracellular vesicles (ADEVs) (A) Transmission electron microscopy (TEM) images showing cup-shaped serum-free and serum-cultured ADEVs (B) Nanoparticle tracking analysis (NTA) showing a significant increase in the median size of serum-free ADEVs compared to serum-cultured ADEVs (**p < 0.01 (C) No difference in particle concentration was identified using NTA between ADEVs (mean = 2 × 1011 particles/mL (D) Size distribution of ADEVs showing a similar size range of in both serum-free and serum-cultured ADEVs (between 50 and 300 nm diameter) a shift toward larger EVs was observed in serum-free ADEVs (E) Representative brightfield images of serum-free astrocytes treated with either 10 μL or 100 μL of serum-cultured ADEVs (2 × 1011 particles/mL) (F) Heatmap showing that serum-cultured ADEVs dose-dependently altered gene expression of astrocyte and inflammatory cytokine markers in serum-free astrocytes (G) Real-time qPCR analysis showing a significant increase in GFAP and S100β gene expression after treatment with 100 μL of serum-cultured ADEVs with an increasing trend also observed in EAAT2 gene expression (**p < 0.01 Data represent mean ± SEM and were analyzed using unpaired t-test (B C) or one-way ANOVAs followed by Dunnett's multiple comparisons test (G) This indicates that EVs may transport bioactive molecules capable of propagating a reactive astrocyte phenotype through mechanisms distinct from those involved in both inflammatory processes and UPR To determine the impact of serum-induced reactivity at the level of the astrocyte whole cell and ADEV proteomes, an exploratory analysis was performed using LC-MS/MS. Based on protein identification in at least 2/3 repeats, significantly more proteins were detected in serum-free cell lysates, with an average of 1,088 proteins detected compared to 680 proteins identified in the serum-cultured cell lysates (Figure 7A Proteomic analysis of serum-free and serum-cultured astrocytes and their ADEVs (A) The number of unique proteins identified in serum-free and serum-cultured astrocyte cell lysates and their EVs (*p < 0.05 Half of the total proteins identified within (B) the cell lysates and (C) ADEVs were detected in both serum-free and serum-cultured conditions (D) Venn diagram displaying the overlap of proteins found within all 4 conditions (E) Identification of EV associated proteins (Category 1 and 2) as well as a lack of contaminant (Category 3) or cell-based (Category 4) proteins Secreted proteins were also commonly found within ADEV samples (Category 5) (F) The majority of ADEV proteins were also found within the Vesiclepedia database (G) Subcellular location of the detected proteins highlighting an increase in cell membrane and secreted proteins within ADEV samples and an increase in nuclear proteins within cell lysate samples (H) KEGG pathway analysis of serum-free ADEVs proteomes highlighting many synapse-related pathways such as glutamatergic and GABAergic synapse pathways as well as many homeostatic pathways such as insulin secretion and axon guidance (I) KEGG pathway analysis of serum cultured ADEVs showing disease pathways such as Parkinson's disease and ALS as well as an enrichment in complement and coagulation cascades Data represent mean ± SEM and were analyzed using one-way ANOVA followed by Tukey's multiple comparison test our proteome analyses confirm that primary human astrocytes have a remodeled proteomic phenotype in response to chronic culture with serum consistent with a shift toward a reactive phenotype we demonstrate that culturing human primary astrocytes in a serum-free medium recapitulates in vivo phenotypic traits as evidenced by morphological and transcriptional analysis Prolonged serum exposure diminishes astrocyte marker expression while short-term exposure mirrors previous findings on astrocyte reactivity suggesting distinct signaling pathways for acute and chronic reactivity We show that chronically serum-cultured astrocytes secrete smaller ADEVs than serum-free astrocytes and can propagate their reactive phenotype to quiescent cells Each type of ADEVs exhibits unique protein profiles offering insights into ADEVs as potential biomarkers of CNS pathologies We therefore speculate that the reduced levels of astrocyte markers in serum condition may be explained by their high proliferative profile in which cells may invest more in the synthesis of proteins related to cell proliferation instead of specific astrocytic markers This hypothesis is supported by the observed enlargement of nuclei and elevated levels of IL-1β and IL-10 in serum-cultured astrocytes characteristic of excessive protein production and an inflammatory secretory phenotype to validate these molecular profiles effectively further investigations necessitate functional validation in vivo we also recommend that more emphasis should be made on multiple structural to validate ADEVs in biological fluids as biomarkers of CNS pathologies but this warrants further functional investigation The concentration of proteins present in serum-cultured medium could potentially trigger astrocyte reactivity to ADEVs and therefore requires further functional investigation transitioning toward serum-free cultures would eliminate this issue in ADEV studies this study demonstrates that human primary astrocytes cultured in serum-free medium are akin to in vivo resting astrocytes and maintaining these cells in serum should be avoided as it induces permanent changes that are specific to human reactive astrocytes These changes are also reflected in their ADEVs suggesting that markers of human primary astrocytes need to be evaluated using multiple molecular and functional parameters before using their ADEVs in functional studies or as predictive biomarkers of astrocyte health The original datasets presented in this study can be found here: https://data.mendeley.com/datasets/ms94f4hz35/1 Ethical approval was not required for the studies on humans in accordance with the local legislation and institutional requirements because only commercially available established cell lines were used Writing – review & editing HB: Writing – review & editing BS: Writing – review & editing PG: Writing – review & editing RM-R: Writing – review & editing DS: Writing – review & editing RL: Writing – review & editing SS: Writing – review & editing The author(s) declare that financial support was received for the research This work was supported by the Nottingham BBSRC Doctoral Training Partnership (grant reference BB/M008770/1 to KW and SS) DS was supported by a MND Association Junior Fellowship (grant reference 970–799) and a Nottingham Research Fellowship (University of Nottingham) HB and KW were supported by MND Association (grant references: 905–792 and 2349–791 and RM-R was supported by MND Scotland (grant reference: MNDS/RP/8390/LAY) Alex Rathbone at the University of Nottingham for assistance with RT-qPCR analysis and Denise Mclean from the School of Life Sciences Imaging (SLiM) at the University Nottingham for assistance with Electron Microscopy (EM) The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher The Supplementary Material for this article can be found online at: 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transcriptional and functional differences with mouse Astrocyte-derived extracellular vesicles: a double-edged sword in central nervous system disorders Layfield R and Serres S (2024) A convenient model of serum-induced reactivity of human astrocytes to investigate astrocyte-derived extracellular vesicles Received: 08 April 2024; Accepted: 17 May 2024; Published: 10 June 2024 Copyright © 2024 White, Bailey, Shaw, Geiszler, Mesquita-Ribeiro, Scott, Layfield and Serres. 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White, a2F0aGVyaW5lLndoaXRlMkBub3R0aW5naGFtLmFjLnVr; Sébastien Serres, c2ViYXN0aWVuLnNlcnJlc0Bub3R0aW5naGFtLmFjLnVr †These authors share senior authorship Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher 94% of researchers rate our articles as excellent or goodLearn more about the work of our research integrity team to safeguard the quality of each article we publish A 34-year-old man was fatally injured on New Year’s Day in Serres following an altercation in a central area of the city although local residents reported hearing gunshots It is believed that at least four individuals were involved in the brawl on the Macedonian city’s main pedestrian street while police continue to investigate the incident Marine Serre continues to challenge fashion storytelling with Heads or Tails a presentation that reshapes how audiences experience a collection Instead of staging a traditional runway show or producing a conventional campaign Serre collaborated with director Tess Lochanski to create a film that occupies space between short cinema and fashion presentation offering a fresh perspective on her Fall Winter 2025 collection the designer rejected any clear-cut distinction between formats choosing instead to construct a world through film that reflects the duality and transformation embedded in the collection the film pushes beyond the usual boundaries of a seasonal release the visual piece offers more than documentation a recurring figure who functions not as a passive presence but as an active symbol The character channels cinematic influences from directors like David Lynch and the 1996 film Irma Vep while presenting a contemporary figure of movement the Ghost reappears as a heroine suspended between dimensions Marine Serre’s choice to place the Ghost at the core of the visual experience reflects an interest in storytelling that goes beyond clothing The character operates as a guide and disruptor drifting through frames with intention and strength Her presence anchors the tension in the film The result doesn’t read as a literal adaptation of a show but rather as a reinterpretation of the emotional and conceptual layers within the collection layering together sonic references that build on the film’s dense visual language a sample of activist Mario Savio’s speech from Shin Sekai and a subtle nod to composer Angelo Badalamenti but creating space for contrast and rhythm The soundscape moves between haunting and assertive it intensifies the energy of the Ghost and her movement through the frame Rather than using models as static visuals and Manon Dubourdeaux move through the film as if navigating a dream built from equal parts fashion and cinema Their performances shift the focus from garments alone to the people who carry them Their roles never settle into one note; instead they shape and reshape themselves throughout the piece giving weight to Serre’s interest in transformation Discover Marine Serre Fall Winter 2025 Collection at Paris Fashion Week the project doesn’t follow any strict category Marine Serre uses the Heads or Tails film to move away from routine formats and explore fashion through layered visuals and characters that ask more questions than they answer crafting an experience that lingers beyond the final frame beautiful as always but I am not sure she can make it anymore as a solo brand Time for Marien to get a Creative Director spot in a big house <3 DSCENE is curated as a daily art, design, fashion & lifestyle destination. 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Home of magazine editions DSCENE and MMSCENE – Click for more about DSCENE and for our Terms of Service Please enter your username or email address to reset your password For FW25 Marine Serre staged a runway show at La Monnaie de Paris yet Serre’s collection challenges the dominance of financial power in fashion Guests were invited with a custom coin stamped with the moon on one side and Serre’s profile on the other less a token of commerce than a talisman of independence Објава коју дели MARINE SERRE (@marineserre_official) The collection marks a defining moment for Serre with seven years of building her brand behind her crafting silhouettes that embody structure and sensuality and references to femme fatales of the 1950s and 1980s shape the collection’s identity A midi dress with futuristic shoulders captures the tension between power and desire while upcycled biker leather is transformed into couture gowns that carry the spirit of rebellion Accessories mirror this bold energy: the AURORA bag and a jewelry capsule collection thatcombines tribal elements with classical motifs These pieces build an image of a woman who is untamed yet composed but Serre approaches it through reinvention rather than restriction as vintage watch bracelets are reimagined as jewelry in a venue synonymous with currency This collection operates like an excavation of the past uncovering fragments of history and transforming them into wearable statements The collection unfolds in a palette of white the latter pulsating with an energy that shifts between passion and power A carmine velvet tracksuit refines sportswear into something fit for the red carpet while red mesh and satin channel a hypnotic intensity The visual language nods to David Lynch’s surreal universe recalls the grandeur of Chesterfield furniture while recycled nylon is crafted to resemble leather The trench coat is deconstructed into layered fragments Marine Serre creates an experience that challenges certainty urging us to 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SUFFOCATION Announces Headlining Dates Around CARNIFEX Tour PARADISE LOST & MESSA Announces European Tour Dates HIGH ON FIRE & NECROT Announce European Tour DANCE GAVIN DANCE Announces Full Lineup For Their Swanfest ANCIIENTS Announces First US Tour In Eight Years Enter your information below to get a daily update with all of our headlines and receive The Orchard Metal newsletter Igorrr also has some new stuff in the works the leaf blower war or the anti-'woke' backlash World Subscribers only Germany's Friedrich Merz is embracing pragmatism World Subscribers only Trump-Carney meeting: Canada seeks reconciliation World Subscribers only Friedrich Merz bets on two private sector converts to revive the German economy and reform the state World Subscribers only Founder of Sant'Egidio community fears next pope could undo Francis's legacy Opinion Subscribers only 'Russian gas and Europe is an old story that ended badly Economy Subscribers only Europe's steel industry flattened by crisis World 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Tracking down the pianos taken from French Jews during the Nazi Occupation Magazine Subscribers only Eve Rodsky the American helping couples balance the mental load Magazine Subscribers only Desecration or more glory Joan Didion's private diaries are revealed Magazine Subscribers only For Jewish cartoonist Joann Sfar 2025."> Pixels Subscribers only Golden Owl solution is revealed but leaves players of 31-year hunt disappointed Pixels Subscribers only Secrets of decades-long Golden Owl treasure hunt to be revealed Lifestyle Inside Chanel's French leather workshops Culture Subscribers only The marvelous bronzes of Angkor on display at the Musée Guimet in Paris the city of Paris granted the young fashion designer 3,000 square meters in the 19th arrondissement This expansive space in her image houses her teams archives and piles of textiles she repurposes for her collections Entering Marine Serre's expansive premises on Rue d'Aubervilliers in northeastern Paris it's impossible not to recall the studio where she started out in 2016 her workspace was 150 times smaller – 20 square meters on the fifth floor of a building with no elevator behind Montmartre a kitchenette and a main room that doubled as a bedroom After graduating from the Belgian school La Cambre and growing up between Corrèze and Creuse in south-central France this is where she created her first collections her first interviews and her first sales to the retail chain Dover Street Market could one dispute her claim that "luxury is space" In one of her previous headquarters – a Haussmannian apartment – she recalled we would always set up camping tables in the middle of the living room But it soon became too cramped." She spoke with her petite figure keen eyes and straightforward way of talking mingled with the "Frenglish" typical of those who constantly switch from one language to another Serre was one of the first to respond to the city of Paris' call for applications to join Métropole 19 – a complex of two five-story rectangular concrete and brick buildings connected by metal and glass suspension bridges Architect Jean-Paul Viguier designed the complex in 1988 to accommodate "small enabling them to remain within the Paris city limits with modern working conditions and low rents," as the architect's agency put it You have 78.59% of this article left to read Lecture du Monde en cours sur un autre appareil Vous pouvez lire Le Monde sur un seul appareil à la fois Ce message s’affichera sur l’autre appareil Parce qu’une autre personne (ou vous) est en train de lire Le Monde avec ce compte sur un autre appareil Vous ne pouvez lire Le Monde que sur un seul appareil à la fois (ordinateur En cliquant sur « Continuer à lire ici » et en vous assurant que vous êtes la seule personne à consulter Le Monde avec ce compte Que se passera-t-il si vous continuez à lire ici Ce dernier restera connecté avec ce compte Vous pouvez vous connecter avec votre compte sur autant d’appareils que vous le souhaitez mais en les utilisant à des moments différents Nous vous conseillons de modifier votre mot de passe Votre abonnement n’autorise pas la lecture de cet article merci de contacter notre service commercial and CIO interviews of medium and large enterprises exclusively from Medical Tech Outlook Company Serres Description Serres is a Finnish family-owned company that focuses on sustainable and safe fluid management with its innovative product Serres suction bags Apart from this it offers other accessories compatible with its flagship product Serres Nemo Sito Web: mythras.it Amministratore: Roberto Livio Mastronardi Descrizione: Mythras è un team di tecnici specializzati nell'assistenza tecnica su strumentazione IVD esperti nell'aiutare i responsabili di laboratorio a garantire la precisione l'accuratezza e la conformità dei loro strumenti È la scelta ideale per numerosi clienti e distributori al servizio delle loro complesse esigenze di assistenza tecnica e ricondizionamento della strumentazione IVD Citazione: “Siamo certificati ISO 9001:2015 per l'assistenza tecnica e il ricondizionamento della strumentazione analitica IVD.” I agree We use cookies on this website to enhance your user experience. By clicking any link on this page you are giving your consent for us to set cookies. More info We use cookies and similar technologies to enhance your experience on our website and to provide personalized content and advertisements By clicking "Accept," you agree to the use of these technologies as described below You can manage your choices or withdraw consent at any time by visiting our Privacy Policy page Essential Cookies: We use essential cookies to make our site work You may disable these by changing your browser settings but this may affect how the website functions Types of Personal Data Processed: We process personal data including unique identifiers and browsing behavior please refer to our Cookie and Privacy Policies Google Services: Please note that we use Google services such as Ad Manager and Google Analytics GA4 to improve our website's functionality and performance Cookies are small text files that are stored on your device (computer They allow the website to recognize your device and store certain information about your preferences or actions We use cookies and similar technologies for the following purposes: You have the option to accept or reject non-essential cookies when you first visit our website You can also manage your cookie preferences at any time by adjusting your browser settings or visiting our cookies consent form Most web browsers allow you to control cookies through their settings You can typically find these settings in the "Options" or "Preferences" menu of your browser Please note that disabling certain cookies may affect the functionality of our website if you would like to share the information in this article www.medicaltechoutlook.com/serres Faced with a complex financial situation and an initial unfavorable decision by the community council on October 9 SATA Group AEON mobilized alongside local stakeholders to guarantee the resort’s future This mobilization enabled us to seal an agreement with the Communauté de Communes de la Matheysine for the opening of a season as early as December 21 L’Alpe du Grand Serre aims to attract a renewed clientele thanks in particular to the innovations promised by SATA Group AEON and an approach geared towards sustainable tourism With 55 kilometers of varied slopes for all levels Alpe du Grand Serre stands out for its accessibility and stunning natural scenery Although modest in size compared to its neighbors it offers spectacular panoramic views and a warm atmosphere popular with lovers of tranquillity and nature the resort attracts families and intermediate skiers while offering an environment conducive to freeride and learning to ski The resort also offers a variety of off-piste activities cross-country skiing and even paragliding for the more adventurous SATA Group AEON plans to exploit all this potential by turning Alpe du Grand Serre into a laboratory for innovation in sustainable tourism. “The Alpe du Grand Serre will be, within our group, a real laboratory where we can test new activities, attract a diversified clientele, and create a lively mountain destination all year round “ stresses Fabrice Boutet “We are honored by the trust placed in us by the Communauté de Communes de la Matheysine and its elected representatives to manage this resort combined with our vision of responsible mountain tourism will enable us to develop the full potential of this destination while preserving its authenticity and exceptional environment.“ Despite an initial unfavorable vote at the community council meeting on October 9 SATA Group AEON mobilized alongside local stakeholders as well as the entire industry – the ESF various support associations and the French government – in a show of solidarity A new consultation resulted in an agreement to reopen the resort for 1 year with the aim of relaunching a public service contract committing the area to the long term The takeover project was unanimously approved by the Matheysine community council SATA Group AEON believes in the opportunity to create a new economic model for the future of the mountains through the Alpe du Grand Serre l’Alpe du Grand Serre could become an example for medium-sized resorts in the French Alps seeking to strike a balance between tourist appeal and respect for the environment SATA Group AEON’s takeover of Alpe du Grand Serre has prompted mixed reactions from mountain enthusiasts and local players Some see it as a temporary reprieve for a struggling resort highlighting the long-term challenges of ensuring the economic viability of small resorts in the face of climate change and changing tourism practices this project represents an unprecedented opportunity to move the mountains towards an alternative model more respectful of ecosystems and better anchored in the local economy driven by a sustainable vision and a willingness to innovate could well transform the Alpe du Grand Serre into an example for other smaller resorts to follow demonstrating that it is possible to reconcile tourist appeal with the preservation of mountain territories resonates in every resort managed by the Group The importance of the resort for the inhabitants of the Matheysine and surrounding communities is obvious It generates essential economic activity for many families accommodation or winter sports-related services sectors SATA Group AEON’s commitment therefore extends to creating opportunities for local players and providing season-long support for summer and winter activities adapted to the modern challenges faced by small ski resorts in France The Alpe du Grand Serre is scheduled to open on December 21 a symbolic date marking a new beginning for this resort with unique potential Visitors will be able to explore freshly prepared slopes and discover new facilities SATA Group AEON intends not only to revive the resort’s activity but also to demonstrate the viability of its sustainable mountain tourism model This vision could inspire other resorts of similar size across the French Alps facing the same economic and environmental challenges A celebrity chef and her husband kick up their heels with a Western-inspired The duo were then properly introduced (most definitely matchmaking instincts were at play) at their building’s AGM. A year later, in 2017, they had their first date at Cassis Bistro DeSousa and Serre had both previously been married and had daughters (DeSousa one so the idea of getting married again didn’t initially come up the couple began to discuss the idea of tying the knot Serre proposed in the same corner seats at Cassis where they had their first date With the wedding set for the summer of 2024 the couple wanted to incorporate their shared love of Western culture and celebrate the city where they met and fell in love And what better way to do that than a Stampede wedding I’ve always loved Stampede and thought it would be a special time to get married and to have our family and friends participate in all things Stampede,” DeSousa says The ceremony was on the rooftop terrace of DeSousa’s East Village restaurant and the bride and groom both wore cowboy boots Rather than a traditional ceremony with a hired officiant and scripted vows DeSousa and Serre opted for a more personal and familial approach — complete with a special surprise planned by DeSousa it was award-winning Canadian country singer Paul Brandt Serre says that moment was the most memorable for him Brandt played “I Do,” an ideal song to celebrate their union had Brandt on hand playing the same song while they renewed their vows after 38 years together The ceremony was officiated by Serre’s best friend whose words inspired laughter and happy tears The cheese layers waterfalled down to an eight-foot-long charcuterie board put together by DeSousa’s Charbar team “It was everything we wanted our wedding to be,” says DeSousa “From the informal ceremony to one big Stampede party at the end This article appears in the January 2025 issue of Avenue Calgary This website uses cookies to improve your experience. We'll assume you're ok with this, but you can opt-out if you wish. Accept Privacy Policy [gravityform id=”48″ title=”false” description=”false”] Do you enjoy strolling among the thousand and one orchids in the Grandes Serres of the Jardin des Plantes? Then you're going to love theTropical Autumn stroll The Muséum national d'Histoire naturelle de Paris is back again this autumn for a plant walk-exhibition that's as magnificent as ever From October 17 to November 25, 2024, during the All Saints' vacation and the month of November Drawing Botany: a lively and fantastic plant exhibition Art and plants mingle and complement each other offering us a truer-to-nature immersion in the midst of botanical species of surprising aesthetic appeal this fourth edition of Automne Tropical aims to further surprise the senses by revealing little-known and original plants the Jardin des Plantes invites the artist Agathe Haevermans for an innovative artistic journey The exhibition invites us to rediscover the art of botany a discipline that combines knowledge of nature and drawing Botanists are given pride of place: we admire species newly discovered by researchers and learn more about the specific characteristics of these plants thanks to the artists' precise drawings These works also recall the history of the natural sciences and the importance of art before the advent of photography and new technologies nature and drawing complement and embellish each other Enthusiasts and amateurs alike are welcome to admire the beauty of nature and discover its secrets and peculiarities This lively experience will delight the curious and plant lovers alike If the experience makes you want to go green there's also an ephemeral boutique where you can take a plant home as a souvenir of this enchanted stroll In the warmth of the Jardin des Plantes' Grandes Serres we feel transported to faraway and mysterious lands You can almost hear jungle animals moving in the trees above you.. A relaxing experience not to be missed this autumn Refer your establishment, click herePromote your event, click here The location, the live band, and this collection all combined to create a fascinating new chapter in the Serre story. Because while her work is very much informed by Paris, it was tangibly enhanced through this fleeting translocation to a dreamy hillside above Florence. N.Hoolywood Test Product Exchange Service Spring 2025 Menswear