Joe Bugyi wanted to expand the transportation company he founded during World War II into Toledo but he didn’t know anyone in the area who then worked for commercial vehicle systems supplier Dana hoping for a referral from someone he trusted The only person his son could offer up was himself So Nagle Jr. left his job, opened the terminal with wife Joanne in 1977, and went to work forming relationships with the local foodservice community. And those connections helped them start their own trucking business, Nagle Toledo in 1984 after Bugyi closed his company due to industry deregulation the firm still is fortified by fast friendships and familial bonds “We’re focused on making sure this company continues to thrive because we have 120 families who count on us,” said Nagle’s son who helped his parents start the company and now serves as president and CEO “We want to make sure they have a good home—one they could potentially retire from.” doing so for decades is exceedingly difficult The 2008 Great Recession forced the refrigerated carrier to downsize the COVID-19 pandemic inflated operating expenses and today’s soft freight market is stifling rates But Nagle’s long-standing emphasis on building relationships is buoying the business “Trucking isn’t a job to us,” Ed said going to work at the truck stop where his parents originally rented an office He helped his parents create Nagle Toledo in June 1984 and then welcomed his three brothers into the family business: Steve a few months later The brothers ran the company together until Mike left in 2008 leaving Ed as sole owner—until the next generation takes up the torch as part of its operations implementation team and Jeff is “doing very well” in financial services I’ll buy it and find someone to run it,’” he said He loves the life and figures he has 10-15 years left in his career But he’s already working to identify his replacement because he cares too much about Nagle’s employees to leave their fate undetermined “We have to make sure this company survives,” he said The operation today includes five companies: Nagle Toledo which maintains the operating authority; Nagle Equipment home to the trucks and trailers; Nagle Leasing including two warehouses; and Nagle Logistics Group Nagle today employs 66 drivers and 115 total people hauling fresh and frozen products for food retailers across the Northeast Nagle moved from a leased space to Walbridge The 15-acre property boasts a 15,000-sq.-ft The combined operation generated $25.7 million in 2023 with $13.6 million in truckload revenue and $10.2 million from third-party logistics services—and Ed is optimistic about further expansion “We expect to see good growth over the next five to seven years in logistics and dedicated transportation and somewhat in over-the-road,” he said The post-2008 “commoditization” of long-haul trucking devalued relationships especially when capacity was overly abundant as it is now in the wake of the spot market high that followed the pandemic That’s why Nagle is focused on cultivating dedicated business which is the group’s fastest-growing segment generating $9 million in service of local manufacturers last year “The dedicated division is important because it’s two partners who depend on each other,” Ed explained “They need us to produce their goods We actually include our profit as a cost of doing business So it’s not as subject to market fluctuations you’re at the mercy of economic pressures.” Dedicated services strengthen relationships providing long-term stability for customers and employees who are then more likely  to provide personalized “extra-mile” care to the people they interact with on a daily basis “The big carriers run phenomenal programs but they’re ‘cookie cutter.’ If you want their best pricing you must do everything the same way as the rest of their customers,” Ed contended along with Nagle’s third-party shop business—which last year generated an additional $1.9 million in revenue—also allows the carrier to withstand persistent freight recessions and keep expanding at a measured pace “We’re not going to grow the fleet just to grow the fleet,” Ed said “What’s important to us is to maintain an OR [operating ratio] that is acceptable Trying to run at 98 or 99.5% just to have bragging rights about how big you are is of no interest to us.” and 12 non-CDL holders who deliver parts in straight trucks—and caring for them is of great interest hiring and retaining dependable drivers is increasingly difficult According to the American Trucking Associations trucking is short 60,000 drivers—and Nagle only hires drivers with at least two years of experience “The biggest challenge we have right now is finding quality drivers,” Nagle COO James White said “And what we’re seeing is drivers have a lot of options so we might get one who is here three months they’re going elsewhere because they were offered a different rate.” The carrier primarily pulls “no-touch freight” from manufacturing facilities to distribution centers so its drivers—who average 62 years of age—don’t do a lot of heavy lifting Nagle drops trailers 75-80% of the time to prevent unproductive detentions and pays OTR drivers a weekly salary while dedicated drivers are paid an hourly rate issued weekly Nagle regularly invests in equipment that appeals to drivers and advanced technology that promotes safety including forward- and driver-facing Samsara cameras that monitor for distracted driving and driver fatigue “That technology is priceless,” said Darrell Tarry The system also unlocks coaching opportunities but Nagle maintains a strict policy on cell phone usage which Ed insists is “the most under-reported cause of accidents in this country.” The first incident results in three unpaid days off the second leads to five—and a third triggers termination the threat of a nuclear verdict is so great that we have an incredible responsibility to all of our employees to make sure a lawsuit doesn’t put us out of business,” Ed said Nagle's fleet of 78 temperature-controlled trailers and 40 dry vans as well as customer trucks and trailers in a mutually beneficial affiliation are maintained by nine mechanics and two parts managers but because we see the pros and cons other fleets experience it helps us make decisions,” Ed said “We’re learning about different types of fuel-water separators the more confidence you have in the decisions you make.” Nagle offers up informed equipment recommendations to customers—whose repeat business bolsters the group’s bottom line Nagle also is extending tractor trade cycles by a year to boost profitability in the soft freight environment so even if we have an unexpected $30,000 repair to make that’s still better than spending $200,000 on a new tractor in this market,” he said And he isn’t overly concerned about maintenance costs rising because the six-bay shop administers an “over-the-top” maintenance program supported by intelligent telematics that enable “predictive” maintenance The carrier runs Thermo King S-700 transport refrigeration units with TracKing telematics “It’s a game changer for me,” Tarry said “It takes what four people used to do down to a simple one-person assignment I check it three times a day to make sure everything is rolling smoothly.” The refrigerated trailers are Utility Trailers equipped with P.S.I tire inflation systems and Samsara tracking devices enough to maintain “ice cream” temperatures due to the S-700’s cooling capacity and flat floors that are well suited for palletized freight They’re heavier than aluminum duct floors but more durable so we’re now hoping to achieve a 10-year [trailer] life,” he said Nagle’s tractors are Freightliner Cascadias equippedmwith Thermo King APUs and Ex-Guard grille guards—an invaluable investment “They have saved us over $100,000 in repairs since we started equipping them,” he maintained He’s equally appreciative of the Cascadia “It’s been a great truck for us,” he said “It offers driver comfort and accessibility and gives us good value.” Nagle sticks with Thermo King for the “phenomenal” aftersales support—and because the manufacturer was the only brand in town when Nagle bought its first truck in 1984 “Toledo has a nice Carrier Transicold dealer now but we’ve maintained such a solid connection with Thermo King and have great familiarity with their product,” Ed said And the carrier now runs Utility trailers because of his appreciation for the Wannemacher family which owns Double A Trailer Sales in Delphos “We’ve followed the product line they carry because of our relationship,” Ed stated “So we typically don’t make decisions based on pricing. Double A is trustworthy and ethical The carrier also depends on its OEM network to inform its approach to zero-emission vehicle adoption “I’m confident our company will never run electric trucks,” Ed predicted “I don’t believe the technology is appropriate or sustainable ATRI published a study saying there aren’t enough rare-earth minerals on this planet to provide all consumers and commercial industries with electric vehicles And we’re just pushing pollution away from the United States to poorer countries that do the mining.” EVs also are heavier and more expensive than diesel-powered tractors so they won’t make any sense until customers demand them “We can’t afford to be the guinea pig for these new technologies,” Ed said Ed is focused on strengthening its most important relationships—the ones with employees and customers—by growing dedicated and third-party logistics services if the company finds the right partner to anchor the facility And association involvement aids their cause uniting stakeholders large and small in the shared goal of improving trucking safety Ed is a former Ohio Trucking Association chairman and he’s the current Truckload Carriers Association vice chair to ATA and he is wholly committed to uplifting Nagle “This is who we are,” Ed  said “Trucking isn’t just in our blood has nearly 20 years of experience as a journalist He spent 15 writing and editing for daily newspapers and began covering the commercial vehicle industry in 2018 He was named editor of Bulk Transporter and Refrigerated Transporter magazines in July 2020 The dates displayed for an article provide information on when various publication milestones were reached at the journal that has published the article activities on preceding journals at which the 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ReginaNews‘One of a kind’: Historic Regina home now occupied by well-known bakeryBy Wayne MantykaPublished: February 27, 2025 at 10:56AM EST Twitter feed ©2025 BellMedia All Rights Reserved Metrics details Identifying molecular alterations occurring during cancer progression is essential for a deeper understanding of the underlying biological processes Here we have analyzed cancerous and healthy prostate biopsies using nanoLC-MS(MS) to detect proteins with altered expression and N-glycosylation We have identified 75 proteins with significantly changing expression during disease progression The biological processes involved were assigned based on protein–protein interaction networks These include cellular component organization Multiple glycoproteins were identified with aberrant glycosylation in prostate cancer where differences in glycosite-specific sialylation and galactosylation were the most substantial Many of the glycoproteins with altered N-glycosylation were extracellular matrix constituents and are heavily involved in the establishment of the tumor microenvironment Pathological Grades (G) range from 1–3 while Gleason Grades (GG) range from 1 to 5 intermediate (G2 and GG2-3) and high-risk groups (G3 and GG4-5) our objective was to identify molecular changes in PCa analyzing a large number (95) of TMA core biopsy samples This allows us to detect relatively small differences in protein abundances with a high statistical power We have compared protein expression levels and changes in N-glycosylation features among various pathological grades of PCa and healthy tissues Workflow of the analysis of the TMA samples The “Results” section is divided into three major parts: (i) the molecular differences between healthy and cancerous prostate tissue; (ii) the molecular changes with PCa grade progression and differences between distinct grades and healthy tissue; (iii) and the biological processes altered in PCa While the first two sections are based on data from both the proteomics (containing protein intensities) and glycoproteomics datasets (containing glycopeptide intensities and metrics calculated from them) the third one is based on proteomics data only Before describing the results of the three aforementioned sections a general characterization of the two datasets (proteomics and glycoproteomics) is provided MaxQuant quantified 653 proteins altogether in the 95 supernatant samples analyzed. From these, proteins that were found in less than 60% of any of the sample groups were excluded. Missing values were then imputed as described in the “Methods” section Altogether 145 glycopeptides were quantified in 95 samples with high confidence corresponding to 22 glycoproteins with 29 glycosites and 53 different glycans Over 75% of the identified glycopeptides carried complex-type glycans More than half of these structures were biantennary while tri- and tetra-antennary types and unmatured structures were also present The average antenna sialylation was 20.1% across all samples while 28.7% of antenna containing structures held at least one sialic acid The average fucosylation was 37.8% across all samples All 29 glycosites identified carried several different glycans and also showed considerable diversity regarding glycan type To reveal changes specific to the distinct glycosites metrics were calculated for them individually as well Volcano plot displaying proteins differentially expressed (fold-change at least 2) between healthy and PCa tissues while blue dots represent proteins underexpressed in PCa In the glycoproteomics dataset, 7 glycopeptides were found with significantly different abundances between the normal and PCa groups (Supplementary Fig. S5) fucosylated complex-type glycans with different levels of galactosylation and sialylation glycopeptide expression was lower in PCa tissues: four glycoforms of Immunoglobulin gamma-1 heavy chain (IGG1) N299 and one glycoform of Prothrombin (THRB) N121 The other two showed higher expression levels in PCa: one glycoform of Microfibril-associated glycoprotein 4 (MFAP4) N137 and one glycoform of Biglycan (PGS1) N270 Significant differences were also detected between normal and PCa tissues when comparing the levels of sialylation, fucosylation, and galactosylation at distinct glycosites. The differences in glycosite-specific sialylation, fucosylation, and galactosylation are summarized in Fig. 3. Glycosite-specific alterations in sialylation (A) and galactosylation (C) between healthy and PCa tissues (with standard error displayed) (D) summarizes the direction and volume of the differences in the case of all three metrics (Normal—PCa) in the case of sialylation and galactosylation they did not To uncover molecular alterations among pathological grades and normal tissue, Analysis of Variance (ANOVA) was performed (FDR controlled at 0.05) on both proteomics and glycoproteomics data separately. For exact parameters see the “Methods” section Significantly changing proteins among different grades of PCa and healthy tissue divided into two sub-groups based on hierarchical clustering: upregulated (A) and downregulated (B) Glycopeptides with significant changes between different Grades of PCa and healthy tissues Glycopeptides are annotated as follows: glycoprotein—glycosite—attached glycan (H hexose Changes in the fucosylation of CO6A2 (A) and in the protein expression of different CO6 subunits (B) between different Grades of PCa and healthy tissues alterations between Gleason grades and healthy tissue were investigated as well The number of samples analyzed in the different GG groups was as follows: 7 in GG2 The data analysis was carried out similarly to that of pathological grades Comparison of the proteomics results based on Grade groups and Gleason grades (A) Venn diagram of proteins identified as significant (B) Classification of the 49 PCa samples analyzed The size of the boxes is proportional to the sample sizes (green—Gleason grades (C) Heatmap of the 57 common proteins in both proteomics datasets G3) and groups created from Gleason grades (GG2; GG3 and GG4; GG5) Most of the underexpressed proteins were associated with cellular component organization (34 out of 51) while the overexpressed proteins were predominantly affiliated with metabolic processes (60 out of 72) As the focus of this paper is on finding potential biomarkers through exploring alterations in the glycosylation between healthy and PCa tissues combined with proteomics data only glycoproteins displaying significant changes are discussed individually the differences in protein expression and glycosylation are both reported and they are compared to relevant previous studies on PCa or cancer in general the most significant biological processes are also discussed These changes however reflect only overall tendencies they are not necessarily true for all of the glycosylation sites This is supported by our data: the average fucosylation level of PPAP N94 increased from 47 to 83% in PCa but significant changes in glycosylation have not been reported yet and we also detected significant changes in both fucosylation and sialylation on POSTN N599 an increase from 24 to 72% and a decrease from 83 to 44% respectively We have found that the sialylation of THRB N121 was downregulated significantly in PCa Our results revealed that both sites of MFAP4 showed modified glycosylation in PCa: decreased sialylation on N87 and increased expression of the glycan N4H5S1F1 on N137 The latter glycoform might be a useful indicator in detecting PCa at an early stage as this increased expression was detected between normal and G1 samples This suggests their potential usefulness as a clinical marker Whether the alterations in the glycosylation of these proteins is PCa specific or not especially in the context of their biomarker status our results indicate that alterations between PCa and Normal tissue glycosylation occur primarily on the glycosite level while overall glycosylation may be unaffected altered glycosylation does not necessarily indicate differential expression on the protein level The glycoproteins with significant differences in glycosylation were all secreted either to blood or the ECM and most of them are characterized as an unfavorable prognostic cancer marker by the Pathology Atlas As altered protein glycosylation in cancer has been proven to be nonrandom this suggests that further investigation of the glycosylation and cancer specificity of these potential prognostic markers and identification of their exact roles is reasonable and could lead to further advancement in understanding the function of glycosylation in cancer development and PCa prognosis and Ammonium-bicarbonate were purchased from Merck (Darmstadt and Iodoacetamide were obtained from Thermo Scientific (Waltham Methanol was purchased from VWR International (Debrecen RapiGest surfactant was obtained from Waters (Milford Four different TMA slides were purchased from US Biomax (Derwood, MD, USA): BNS19011, PR481, PR483c, PR633. All of them contained formalin-fixed paraffin-embedded (FFPE) cores with a diameter of 1.5 mm and a thickness of 5 μm. The specification sheets are available at https://biomax.us with information about each core including age Each TMA core contains on average approximately 1 µg protein the TMA slides were baked at 60 °C for 2 h following the supplier’s instructions to prevent tissue detachment de-paraffinization was carried out by incubating the slides in different solvents/solutions sequentially as follows: xylene for 2 × 3 min 10 mM NH4HCO3 (water) for 5 min and finally water for 1 min the slides were placed in antigen retrieval buffer (20 mM Tris–HCl the proteins in TMA cores were reduced using RapiGest and DTT in 1 µL of 20% glycerol for 20 min at 55 °C then alkylated using IAA in 1 µL of 25 mM ammonium bicarbonate (ABC) puffer and 20% glycerol for 20 min at room temperature in the dark each one lasting for 40 min at 37 °C in a humidified box LysC-Trypsin mixture was added in a 1:25 ratio the extraction of the protein digest was done by repeatedly pipetting 1 µL 10% acetic acid extraction solvent five times on the cores and clean-up was performed using C18 spin columns (Thermo Scientific) using the manufacturer’s protocol The resulting samples were dried down and stored at -20 °C for further usage Samples were reconstituted in 15 µL 1% FA and 150 µL ice-cold acetone was added and the solution was stored at -20 °C overnight Then the samples were centrifuged at 13,000 g for 10 min then resuspended in 10 µL of injection solvent and subsequently stored in the autosampler unit until analysis Samples were analyzed using a Maxis II QTOF instrument (Bruker Daltonik GmbH Germany) equipped with CaptiveSpray nanoBooster ion source coupled to a Dionex UltiMate 3000 RSLCnano system (Sunnyvale Peptides were separated on an Acquity M-Class BEH130 C18 analytical column (1.7 μm MA) using gradient elution (isocratic hold at 4% for 11 min then elevating B solvent content to 25% in 75 min and to 40% in 15 min) following trapping on an Acclaim PepMap100 C18 (5 μm Solvent A consisted of water + 0.1% formic acid Solvent B was acetonitrile + 0.1% formic acid and the sample loading buffer was 0.1% TFA and 0.01% heptafluorobutiric acid in water with a dynamic MS/MS exclusion of the same precursor ion for 2 min or if its intensity is at least 3 times larger than previously Preferred charge states were set between + 2 and + 5 MS spectra were acquired at 3 Hz in the 150–2200 m/z range while MS/MS spectra at 4 or 16 Hz depending on the intensity of the precursor Mixed energy spectra were collected at 100% collision energy for 80% of the cycle time and 50% collision energy for 20% of the cycle time spectra were recorded over the mass range of 300–3000 m/z at 1 Hz raw data were recalibrated using the Compass DataAnalysis software 4.3 (Bruker Daltonics The software identified the glycopeptides according to their exact mass The minimum required interaction score was set to the highest confidence (0.900) for active interaction sources “Textmining” was excluded Experimental data has been submitted to the MassIVE data repository with the ID: MSV000087329 EAU-ESTRO-SIOG guidelines on prostate cancer Artificial intelligence assistance significantly improves Gleason grading of prostate biopsies by pathologists Underestimation of Gleason score at prostate biopsy reflects sampling error in lower volume tumours Effects of replacing PSA with Stockholm3 for diagnosis of clinically significant prostate cancer in a healthcare system–the Stavanger experience Advantages of replacing the total PSA assay with the assay for PSA-α1-antichymotrypsin complex for the screening and management of prostate cancer Recent advances in image-guided targeted prostate biopsy and combined biopsy for prostate cancer diagnosis Artificial intelligence for diagnosis and grading of prostate cancer in biopsies: A population-based Automated deep-learning system for Gleason grading of prostate cancer using biopsies: A diagnostic study Current and future perspectives of liquid biopsies in genomics-driven 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N-Glycosylation of IgG Immunoglobulin and its clinical significance. J. Biomed. 4, 35–43. https://doi.org/10.7150/jbm.33922 (2019) Integrated analysis of microfibrillar-associated proteins reveals MFAP4 as a novel biomarker in human cancers Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma A pathology atlas of the human cancer transcriptome Urinary glycoproteins associated with aggressive prostate cancer High-throughput detection of low abundance sialylated glycoproteins in human serum by TiO 2 enrichment and targeted LC-MS/MS analysis: Application to a prostate cancer sample set MaxQuant enables high peptide identification rates individualized ppb-range mass accuracies and proteome-wide protein quantification Accurate and sensitive peptide identification with Mascot Percolator Ggplot2: Elegrant Graphics for Data Analysis (Springer Byonic: Advanced peptide and protein identification software R: A Language and Environment for Statistical Computing RStudio: Integrated Development Environment for R Systematic evaluation of normalization methods for glycomics data based on performance of network inference STRING v11: Protein–protein association networks with increased coverage supporting functional discovery in genome-wide experimental datasets Download references is grateful for funding by the National Research Development and Innovation Office (OTKA PD 121187 and OTKA FK 131603) and acknowledges the support of the János Bolyai Research Scholarship of the Hungarian Academy of Sciences 2018-1.2.1-NKP-2018-00005 has been implemented with the support provided from the National Research Development and Innovation Fund of Hungary financed under the 2018-1.2.1-NKP funding scheme Faculty of Chemical Technology and Biotechnology Budapest University of Technology and Economics 1St Department of Pathology and Experimental Cancer Research annotated the tissue biopsy digital images All authors read and approved the final manuscript The authors declare no competing interests Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41598-021-95417-5 Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. 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Volume 8 - 2021 | https://doi.org/10.3389/fnut.2021.702352 This article is part of the Research TopicRecent Breakthrough in Gluten ContaminationView all 9 articles The use of pure oats (oats cultivated with special care to avoid gluten contamination from wheat and barley) in the gluten-free diet (GFD) represents important nutritional benefits for the celiac consumer emerging evidence suggests that some oat cultivars may contain wheat gliadin analog polypeptides it is necessary to screen oats in terms of protein and epitope composition to be able to select safe varieties for gluten-free applications The overall aim of our study is to investigate the variability of oat protein composition directly related to health-related and techno-functional properties Elements of an oat sample population representing 162 cultivated varieties from 20 countries and the protein composition of resulting samples have been characterized Size distribution of the total protein extracts has been analyzed by size exclusion-high performance liquid chromatography (SE-HPLC) while the 70% ethanol-extracted proteins were analyzed by RP-HPLC Protein extracts separated into three main groups of fractions on the SE-HPLC column: polymeric proteins avenins (both containing three subgroups based on their size) representing respectively 68.79–86.60 and 2.89–11.85% of the total protein content The ratio of polymeric to monomeric proteins varied between 1.37 and 3.73 Seventy-six reversed phase-HPLC-separated peaks have been differentiated from the ethanol extractable proteins of the entire population Their distribution among the cultivars varied significantly The number of appearances of peaks also showed large variation: one peak has been found in 107 samples which appeared in less than five cultivars An estimation method for ranking the avenin-epitope content of the samples has been developed by using MS spectrometric data of collected RP-HPLC peaks and bioinformatics methods Using ELISA methodology with the R5 antibody a high number of the investigated samples were found to be contaminated with wheat The benefits of both applications of oats as human food sources are directly related to the protein composition of the oats used producing these food products: the inclusion of oats in the diet of celiac patients has been a controversial issue Oats are a less likely candidate to trigger CD due to their protein composition all of the important techno-functional properties of oats are directly related to the ratio of polymeric and monomeric proteins in the sample Oats are, in general, considered to have low CD-triggering potential due to their lower prolamin content, higher digestibility, and lower affinity to MHC (Major Histocompatibility Complex) molecules associated with CD compared with that of wheat prolamins (24) The legal gluten-free threshold of 20 mg/kg gluten applies to these oat products as well Although pure oats are considered to be safe for most patients with celiac, there are a number of studies suggesting that oats may be able to trigger CD on their own, but only affected the minority of the population with celiacs connected to individual sensitivity and the condition of the intestine (39). In a study by Lundin et al. (40) a single patient developed complete villous atrophy This patient produced T cells that showed affinity to avenins and were used to identify two avenin epitopes (PYPEQEEPF and PYPEQEQPF) that may have been responsible for triggering villous atrophy These results were limited to this single patient but they raised questions about the presence of celiac-related epitopes in oat avenins there is a great variety of potential immune reactivity of oat cultivars which can generate a higher or lower degree of immune response in patients with celiac disease The overall aim of our study is to demonstrate the variability of oat protein composition directly related to health-related and techno-functional properties we summarize our findings related to genetic factors in an international population of different oat cultivars that have been analyzed using a complex relatively fast and cost-effective protein separation methodology suitable for characterizing large sample populations and the resulting data have been evaluated While the data collected in this study on the overall protein composition including the ratio of polymeric to monomeric oat proteins can be directly related to functional properties the results of the detailed analysis of avenin proteins can help breeders to select oat lines with suitable storage protein composition monitoring the effects of growing conditions on the protein composition of oat as well as the relationships between the protein composition and the techno-functional properties is in progress and planned to be reported in subsequent publications The oat samples were derived from small plot field growing samples were stored in a dry and cold warehouse The dehulling was made with Satake grain testing mill TM-05 (Satake Engineering Co and grinding of hulled grains was carried out with a Retsch MM 400 ball mill (Retsch GmbH Germany) in a gluten-free laboratory environment which was monitored with the R-Biopharm RIDASCREENRIDA®QUICK Gliadin test stripes (Art The protein content of oat flours was determined by the Dumas method (N × 5.95), an adaptation of the AOAC official method (51) using an automated protein analyzer (LECO FP-528 The final SE buffer solution was prepared by mixing 90 ml of solution A + 110 ml of solution B + 600 ml MQ H2O + 4-g SDS Single grains from different samples were placed in 2 ml Eppendorf tubes with a 72-mm-diameter steel ball bearing placed on top of the grain The tubes were lysed using a Qiagen®TissueLyser II (Qiagen GmbH Germany) at 27 strokes/s frequency for 7 min Flour from each tube (10 mg) was weighed in fresh 2 ml Eppendorf tubes and 1 ml of an SE-HPLC extraction buffer was added to each tube Vortex-Genie®2 at setting 6 for 30 min They were subsequently centrifuged for 15 min at 13,000 rpm The supernatant was then aspirated using a 1 ml syringe The supernatant was then passed through a 0.45 μl filter into an HPLC vial The vials were placed in an Agilent Technologies 1200 series HPLC instrument and were analyzed using the following parameters: a Mobile Phase of 50% acetonitrile (ACN) with 0.1% trifluoroacetic acid (TFA) and 50% water HPLC grade with 0.1% trifluoroacetic acid (TFA) was used The SE column (Agilent AdvanceBio Sec 300A 4.6 × 300 mm) was washed for 60 min with 100% water to 100% acetonitrile and stabilized for 1 h before commencing the analysis at 120-bar pressure; the injection volume was 10 μl at a flow rate of 0.350 μl/min The SE-HPLC separation resulted in 10 peaks (P1-P10) polymeric globulin proteins eluted first (P1-P5) while the four latest eluted little peaks (P7-P10) (integrated together) contained the soluble non-avenin proteins About 10 μl of extracts were injected into a C18 reversed-phase ZORBAX 300SB-C18 column (4.6 mm × 150 mm maintained at 60°C column temperature and at 50-bar column pressure The applied eluents were 67% ultrapure water (Buffer A1) and 33% acetonitrile (Buffer B1) The separation was carried out using a linear gradient from 33 to 80% Buffer B1 over 65 min at a flow rate of 1 ml/min RP-HPLC analyses have been carried out with three replicate injections from two replicate extracts In order to detect gluten contamination from wheat oat samples were analyzed with the R-Biopharm RIDASCREEN® Gliadin assay (catalog number: R7001 Extraction and the ELISA procedure were carried out in line with the kit instructions 1 g of oat flour samples was weighed in 50 ml Falcon tubes catalog number: R7016) was pipetted to each sample under a chemical hood the samples were incubated at 50°C for 40 min in a shaking water bath (OLS Aqua Pro After cooling the samples to room temperature 30 ml 80 V/V% ethanol was added to the samples followed by 1 h of shaking on a table-top shaker (1,500 rpm The samples then were centrifuged for 10 min at 2,500 × g at room temperature (LISA Supernatants were diluted 1:12.5 with the sample diluent solution provided to the kit (the concentrate was pre-diluted prior to use according to the kit manual) About 150 μl of kit standards and samples were loaded to a transfer plate in duplicate 100 μl of each sample and standard was transferred to the ELISA plate with a multichannel pipette The plate was incubated for 30 min at room temperature and then was washed with the pre-diluted wash buffer provided for the assay in line with the kit instructions (ELx50 automatic plate washer 100 μl of the pre-diluted conjugate was added to all wells followed by 30 min of incubation at room temperature 50 μl substrate and 50 μl chromogen were added to all wells and the plate was incubated for 30 min at room temperature covered by aluminum foil 100 μl of stop solution was added to all the wells and absorbance values were obtained at 450 nm using a plate spectrophotometer (iMark Data were analyzed with the Microplate Manager 6 software (BioRad USA) using the cubic spline fit to create a standard curve The results were the subject of further calculations to obtain the reporting unit of mg/kg gluten as per the kit instructions The immunodominant T cell epitopes of oat DQ2.5-ave-1a (PYPEQEEPF), DQ2.5-ave-1b (PYPEQEQPF) (56, 57), DQ2.5-ave-1c (PYPEQEQPI) (48), and DQ2.5-ave-2 (PYPEQQPF) were predicted, and the epitope containing avenin levels in different oat varieties was calculated based on the study by Tanner et al. (58). Sollid et al. (47) determined the celiac disease–relevant internationally agreed T cell epitopes recognized by CD4+T cells The study of Tanner even included the DQ2.5-ave-2 that contained only the minority of the investigated oat varieties calculated from their amino acid composition and converted to [mg/100 g sample] units by multiplying the mg/100 g avenin values by the SE-HPLC-based avenin content and by the protein content of the samples Using proteomics data of Tanner in such a way is based on the assumption that their data which are based on the detailed study on a single cultivar (cv is representative for oat cultivars in general The approach to the prediction of epitopes from RP-HPLC data is strictly reliable when these data would be supported and confirmed by amino acid sequence data demonstrating (at least in a representative number of cultivars) the actual presence and amounts of intact avenin epitope sequences in the distinguished HPLC peaks the predicted epitope levels can be interpreted as the measure of the possible variation of epitope contents in the cultivars in the sample population rather than the exact epitope levels in the individual samples The cumulative amounts of the presumably immune reactive avenin proteins per variety were determined and expressed as a percentage of the sample mass by combining the peak data of RP- and SE-HPLC separation and protein content of the samples In the cases of both SE- and RP-HPLC analyses and coefficient of variation have been calculated based on the six replicate data derived from the three replicate injections of two replicate extracts The calculations have been carried out using MS Excel functions Sample groups have been characterized by the variation of the above-mentioned mean values of different protein compositional data different notations are used for describing the variation among the replicate measurements of a given sample (mean and cv) and the variation among the means of different measurements in a group of samples (mean In case of parameters derived more than one, standard deviations were calculated based on the Gaussian error propagation law (59) from the means and standard deviation values (σ) from the individual parameters: in case of the cumulative amount of epitopes the geometrical mean of the four standard deviations were used while the following equation was used for the determination of the standard deviation of the avenin levels in mg/100 g samples unit: where nA and nB are the number of peaks in samples A and B, nA, B is the number of peaks with identical elution times in samples A and B, ei is a weighting factor describing the relative intensity of peaks with identical elution time. Cluster analysis was carried out applying the similarity matrices with the Morpheus R package (https://software.broadinstitute.org/morpheus/) ANOVA test and multiple comparisons of mean values based on the least significant difference (LSD) by Student t-test were carried out as implemented in the NCSS 2021 Statistical Software (2021), (NCSS, LLC. Kaysville, Utah, USA, ncss.com/software/ncss) Protein composition of the oat flour samples has been characterized on two levels: distribution of the total protein content after size-based separation was determined with SE-HPLC followed by the RP-HPLC-based determination of the qualitative and quantitative composition of the avenin fraction More than 99% of the total amount of oat flour proteins has been extracted in the first step of the extraction procedure of Gupta et al. (52) Comparison of samples has been carried out clearly indicating that the ethanol-soluble proteins are eluted as one single peak (P6) The typical SE-HPLC profile of the total oat protein extract (T) and 70% ethanol extract of oat flour (E) The reproducibility of the peak intensity measurements has been monitored by calculating the mean, stdev, and cv values for each peak from their six replicate analysis data (Supplementary Table 2) Based on the averages of cv values calculated from the data of the 6 replicates among the 162 samples and non-avenin monomeric protein group measurements are 5.018 The distribution of the proteins among the three main groups and inside of the polymeric fraction shows a well-defined trend all around the 162 samples The polymeric fraction represents about three-quarters of the total protein content (Mean: 73.14% max: 86.60%); the amount of the avenin fraction is varied between 8.86 and 27.72% (mean: 19.38%) while the amount of the monomeric globulin fraction is between 2.89 and 11.85% (mean: 7.29) the relative amounts of the five subfractions of the polymeric proteins show a P1 < P2 > P3 >> P4 ⋙ P5 trend Comparing the relative distribution of the proteins in the different geographic regions (R1-R8), it was found (Table 1) that the total amount of polymeric proteins and its distribution among the five subfractions (with the exception of P2) and the ratio of the polymeric to monomeric globulin proteins show significant differences among the eight geographic groups Statistical analysis on the variation of the size-based distribution of the total proteins of oats samples among the different geographic regions Compared to the data in the rest of the geographic groups the highest amount of polymeric proteins (means: 75.10 and 74.65%) and polymeric to monomeric protein ratio (means: 11.89 and 13.47%) were found in the R1 and R7 The cause of these values derived from significantly higher amounts of P1 fraction found in the R1 and R7 groups (means: 25.65 and 17.35% compensated only partly with the significantly lower values of P3 (6.29 and 8.94% Beyond the apparently uniform avenin levels observed at the comparison of mean values in the different geographic groups some extremely low (AUS05: 8.86%) and extremely high (AUS14: 27.72%) avenin contents were observed These cultivars could have great potential to be applied to nutrition-related breeding programs characterizing individual RP-HPLC peaks by using the mass spectrometric methodology As it was observed in previous studies (for example most of the avenin polypeptides are eluted in two elution time intervals: 20 peaks have been found in the 25.75-32-min interval and 37 in the 38–47.25-min interval representing the 45.58 and 48.42% of the total avenin content The number of appearances of a peak with a given retention time in different samples was found to be extremely variable There are three peaks with the retention times of 25.75 and 35.00 min found only in three cultivars while the peak with the retention of 42.39 min was found in 107 samples Characteristic peaks in the six similarity clusters of avenin protein RP-HPLC profiles and the origin-based distribution of oat samples among the clusters Some interesting observations can be made, investigating the distribution of samples in the different clusters based on their origin (Table 2) and R8 groups are scattered in different clusters most of the samples in R6 group are together in Cluster C but not in A or B cluster; 18 from the 40 samples in R7 can be found in Cluster F and 36 from the 39 samples in R1 are located in Cluster A Differences among the avenin composition of the samples are significantly enlarged if the amounts of the different peaks are used in similarity calculation (S'%) instead of the presence/absence-based comparison (S%) Expression levels of avenins with the same retention times in different samples have been found largely not uniform among the peaks The reproducibility of the peak intensity measurements has been monitored through the 1,530 peaks found in the whole sample population by calculating the mean and cv values for each peak from their six replicate analysis data resulting in a 7.18% for the average value for the cv values The r2 value between elution times and cv values of peak intensities of peaks eluted at a given elution time was found to be 0.0036 while a strong negative correlation was found between the peak intensities and their reproducibility (r2 = 0.7934): in the 10–15% peak intensity interval 11% in the 5–10 and 10–15% intensity intervals Applying the data provided by Tanner et al. (58) for the composition of avenin fraction of the oat variety cv. the amounts of the celiac-related oat epitope-containing components of the 162 oat samples have been predicted based on their RP-HPLC analysis results containing conserved avenin types: peak 3 (R.T Peak 3 contained the gliadin-like avenin (L0L6J0) peak 8 contained an Asat-Prolamin10 protein and a 23539 Da avenin (Q09072) with an alternative name celiac immunoreactive protein 2 or gamma-avenin-3 (Q09097) and an Asat-Prolamin71 protein the predominant avenin epitope is the DQ2.5-ave-1a (PYPEQEEPF) the DQ2.5-ave-1b (PYPEQEQPF) and DQ2.5-ave-1c (PYPEQEQPI) all the above mentioned three avenin epitopes occurred Satisfactory reproducibility has been observed for the individual and cumulated epitope levels (average cv values calculated for the 162 samples for the DQ2.5-ave1a and DQ2.5-ave1c epitopes and for their cumulated value: 0.096 The cv values for the avenin levels expressed in [mg/100 g sample] units varied between 0.003 and 0.129 with an average of 0.062 For the pure oat line development study, a small population consisting of 32 Australian and 35 Hungarian samples (Supplementary Table 5) was selected from the basic population for ELISA testing Samples were selected to cover a wide range of crude protein content using samples with sufficient available amounts The presence of potential gluten contamination from other cereals was tested with the R5 ELISA method of R-Biopharm 19 Australian and 24 Hungarian samples of the investigated oat varieties were uncontaminated deemed appropriate for the requirements of pure oat cultivation in terms of purity Our results confirm that gluten contamination of oats is a serious problem and must be carefully addressed when providing seeds for growing pure oats The aim of our work was to carry out a high-throughput analytical screening completed with immune analytic measurements to develop a reliable prediction method for estimating the amount of avenin proteins and those that contain celiac-related epitopes This special prediction method utilizes the combined application of SE- and RP-HPLC separation of the total protein content of the oat flour samples and differentiates the absolute levels of the four main avenin epitopes of the samples and also provides the celiac-related epitope containing avenin content in the oat flour (g/100 g) Interestingly, no application of SE-HPLC on characterizing oat proteins is reported in the critical work of Sunilkumar and Tareke (62) which reviewed the analytical methods for measurement of oat proteins by covering 2,000 works published between 1970 and 2015 However, the application of size-related analytical techniques like SE-HPLC has a large potential to be used in selecting oat lines for industrial ingredient use (61) and oat flours using mixtures of four cultivars each to account for the genetic variability between different cultivars including the most relevant cultivars in Germany 2012 It reveals that the examined commercial oat flour samples were the high variability of avenin fraction composition and biodiversity of cultivated oat varieties are in agreement with the results of several research groups who are experts of this field significantly reduce the immunoreactivity of avenins and thus of oat-based foods These findings were confirmed by the study of Hardy and co-workers in a large-scale oat challenge proved that the ingestion of oat is safe for patients with celiac without intestinal damage and serological relapse Because pure oat consumption carries a low risk for patients the researchers declare that the strict control of production systems of pure oat is of utmost importance and the regular follow-up of the patients with CD is recommended Based on the R5 R-Biopharm RIDASCREEN® Gliadin assay of the selected subpopulation showed that 35% of the samples were contaminated This highlights the necessity of improving the pure oat line and developing very sensitive and specific analytical methods for the sake of food safety All observations described above were derived from a reasonably large study where the carefully executed experiments were carried out with 2 × 3 replicates The resulting data have been thoroughly analyzed statistically taking into consideration the non-trivial characteristics of cumulative and complex parameters where the actual results were derived from several independent measurements with experimental errors The reproducibility of the two chromatographic separations seems to be satisfactory with the relative errors being under 12% These positive experimental characteristics do not avoid two principal limitations of the prediction method introduced here: The reliability of the predicted information derived from this prediction process strongly depends on the validity of the assumption that the proteomic data (derived from the analysis of one single cultivar) are representative of oat cultivars in general The predicted epitope levels should be validated by detailed proteomic analysis to avoid this limitation Because of the limited resolution of the RP-HPLC separation of avenin proteins the predicted epitope levels have to be interpreted as upper limits large qualitative and quantitative differences have been observed in the avenin composition of the samples investigated: both the individual and cumulative amounts of the four oat avenin epitopes show large variation while certain cultivars do not contain all the four different epitopes there is no variety among the 106 samples not containing any DQ2.5-ave epitopes with the maximum number of 46 cultivars (28.40%) containing 26–50 mg/100 g epitopes Two cultivars have been found with epitope levels of less than 5 mg/100 g (HUN31 and AUS04); these rarely found low levels could be utilized in breeding for healthy oat varieties Variation of the amounts of celiac-related avenin epitopes among 106 oat samples As the results of the large variation of epitope levels in the whole sample population, significant differences among the origin-based subgroups can be observed (Table 4) for the amounts of DQ2.5-ave-1a and DQ2.5-ave-1c The highest F value (5.6672) was found for the cumulative epitope levels data expressed as [mg/100 g sample] what can be explained by the fact that these values do not only derive from the variation in avenin composition but they are varied by the total amount of avenin proteins as well as the protein content of the samples shows significantly lower levels in the Australian samples (47.52 mg/100 g sample) compared with the South African and South American samples (117.92 and 123.61 mg/100 g samples) ANOVA comparison on the predicted celiac-related avenin epitope contents of samples in the eight regions of origin The celiac-related epitope content of an oat sample is determined by its avenin composition, but the relative expression levels of both avenin- and non-avenin-type polypeptides can overwrite the ranking of the overall epitope levels in the samples, as it is illustrated in Figure 2: In the samples in the circled interval of the figure the epitope levels expressed in mg/100 g avenin protein unit are misleading underestimating the amount of epitopes taken by the consumed oat Demonstrating the importance of expression levels of avenin and non-avenin proteins in the ranking of relative celiac epitope amounts of oat samples by the comparison of ranking samples based on the amount of celiac-related epitopes expressed in (mg/100 g avenin) and (mg/100 g sample) units Relative celiac-related epitope levels in samples in the red circle are largely underestimated by the simple comparisons of the epitope levels in the samples not taking into account the total protein content and its avenin content Circled data with red and green indicate under- and overestimated epitope levels using [mg/100 g avenin] units not considering the contribution of protein content and avenin content of the sample As it is well established for all cereal crops both the protein content and protein composition are highly affected by the growing conditions including both environmental and agrotechnical factors Based on an unpublished large project carried out in our laboratory investigating the alteration of the protein composition of 180 oat cultivars under rainfed and irrigated conditions protein content of the samples of the same cultivar can be altered by 15 relative percentages while the ratio of polymeric and avenin proteins can vary by 38 relative percent caused by the water availability The observation illustrated in Figure 2 underlines the need for quantitative characterization of the overall protein composition rather than simply concentrating on the avenin composition estimating the celiac-related epitope content of oat samples Utilization of oats lines for human consumption requires the use of a reliable methodology of monitoring the presence and quantity of epitope containing components in the samples and a better understanding of chemical composition and technological properties is needed Both of these aspects require the active use of quantitative protein analytical techniques for the characterization of the whole spectra of oats proteins The application of detailed protein composition data has huge potential both in evaluating oats breeding lines in the pre-breeding selection phase and in monitoring oats-containing products in the food industry The combination of SE- and RP-HPLC methodology with active use of available proteomic data seems to be a satisfactory tool for these types of applications Relating SE-HPLC-related quantitative protein analytical data to functional properties of oat samples like water and oil-binding capacity emulsifying and foaming properties and even rheological properties of oats-containing doughs are in progress to utilize the data collected in this study Despite these valid and serious above mentioned limitations of the prediction method developed in this work with the lack of any other (better) relatively high throughput and cheap method what is applicable to large sample populations the method is suitable to be used as a preselection screening tool in oat breeding in its present form already Ongoing attempts to carry out further individual RP peak proteomic validation studies on different oat varieties will make our prediction method much more accurate in the future The original contributions generated for the study are included in the article/Supplementary Material further inquiries can be directed to the corresponding author/s and OV provided samples and sample preparation and ST performed experiments and analyzed data All authors contributed to the article and approved the submitted version The authors declare that this study received funding from the “GalgaGabona” Project (2017-1.3.1-VKE-2017-00004 and from the BME-Biotechnology TKP grant of EMMI (BME TKP-BIO) GG was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences by the ÚNKP-18-4-BME-393 and ÚNKP-20-5-BME-292 National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research The funders were not involved in the study design the writing of this article or the decision to submit it for publication and SP were employed by company Cereal Research Non-Profit Ltd KS was employed by company First Pest Mill and Bakery Ltd The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher The Supplementary Material for this article can be found online at: 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Scholar in relation to their safety for celiac patients Long-term consumption of oats in adult celiac disease patients Identification and analysis of multivalent proteolytically resistant peptides from gluten: implications for celiac sprue Tömösközi S and Békés F (2021) Investigation of Protein and Epitope Characteristics of Oats and Its Implications for Celiac Disease Received: 29 April 2021; Accepted: 23 August 2021; Published: 29 September 2021 Copyright © 2021 Gell, Bugyi, Florides, Birinyi, Réder, Szegő, Mucsi, Schall, Ács, Langó, Purgel, Simon, Varga, Vida, Veisz, Tömösközi and Békés. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) distribution or reproduction in other forums is permitted provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited in accordance with accepted academic practice distribution or reproduction is permitted which does not comply with these terms *Correspondence: Gyöngyvér Gell, Z2VsbC5neW9uZ3l2ZXJAYXRrLmh1 Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher 94% of researchers rate our articles as excellent or goodLearn more about the work of our research integrity team to safeguard the quality of each article we publish 7-foot-tall model rocket for their rocketry badges assembling of the motor and launching the rocket The large rocket was built for the last stage in the rocketry program in which cadets build a rocket capable of carrying a small payload to 300 feet Bugyi and Thrasher carried a large payload to 3,000 feet The launch was a success and the rocket was returned on a 45-inch parachute to a field nearby One of the missions of the Civil Air Patrol is aerospace education The cadet model rocketry program is one small part of that mission The local squadron meets 6:30 to 8:30 p.m. Monday evenings at the East Bangor Fire Station. For information, contact Capt. Rod Thrasher at 570-242-5722 or cathrasher@yahoo.com or visit capsquad807.webs.com. Information on the national Civil Air Patrol program can be found at gocivilairpatrol.com Use of and/or registration on any portion of this site constitutes acceptance of our User Agreement, (updated 8/1/2024) and acknowledgement of our Privacy Policy, and Your Privacy Choices and Rights (updated 1/1/2025) © 2025 Advance Local Media LLC. All rights reserved (About Us) The material on this site may not be reproduced except with the prior written permission of Advance Local Community Rules apply to all content you upload or otherwise submit to this site YouTube's privacy policy is available here and YouTube's terms of service is available here Ad Choices Air Force Auxiliary Civil Air Patrol Local Squadron 807 launched a large model rocket seven-foot tall model rocket for their rocketry badges assembly of the motor and launch of the rocket They have to build a rocket capable of carrying a small payload to 300 feet they carried a large payload to 3,000 feet "It was an awesome experience that has grown to become a hobby." One of the missions of the Civil Air Patrol is aerospace education The cadet model rocketry program is just a small part of that mission The cadets must build and fly five rockets from simple and small to larger and more complex They must also pass three written tests: history of rocketry laws of rocketry and modern model rocketry After they complete all of the requirements they are awarded the CAP Model Rocketry Badge The objective of the aerospace education mission of CAP is to promote an understanding and appreciation of the impact of aviation and aerospace in participants' everyday lives The local squadron meets Mondays from 6:30 to 8:30 p.m Rod Thrasher at 570-242-5722 or cathrasher@yahoo.com or www.capsquad807.webs.com Information on the national Civil Air Patrol program can be found at www.gocivilairpatrol.com Community and the strong foundations provided by faith are among the touchstones for the new Executive Director of the Diocese of Saskatoon Catholic Foundation Raissa Bugyi will begin her new role July 5 Her appointment was announced May 27 by Ray Kolla Chair of the Diocese of Saskatoon Catholic Foundation the fund-raising arm that supports the work of the Roman Catholic Diocese of Saskatoon “On behalf of the Catholic Foundation and Bishop Mark Hagemoen I am delighted to announce the appointment of a new Executive Director for the Diocese of Saskatoon Catholic Foundation,” said Kolla in a letter to parishes “Raissa comes to us with a deep understanding and appreciation for the importance of community and faith Most recently she has served as Executive Director of Preston Park II Retirement Residence in Saskatoon and has extensive experience in marketing and management,” Kolla said Announcement from Diocese of Saskatoon Catholic Foundation – PDF Raissa Bugyi will succeed Executive Director Don Gorsalitz, who has served as the Executive Director of the Catholic Foundation since it was established. Gorsalitz is leaving that role in order to focus more time on his consulting firm, DCG Philanthropic Services Inc. On behalf of the Catholic Foundation board and the diocese of Saskatoon Kolla thanked Don Gorsalitz for his “dedication commitment and outstanding service.” “Don’s generosity in providing his expertise professionalism and passion in a volunteer capacity leading the Catholic Foundation through its establishment and growth and his many stewardship and fund-raising accomplishments in our diocese over the years has had an immeasurable impact on our diocesan Church and the wider community,” said Kolla Bishop Mark Hagemoen said: ““We will miss Don Gorsalitz very very much He has contributed so much to building our diocese I am very grateful that God has provided us Raissa to build on the good work and strong foundation established by Don.” Raissa (pronounced “Ray-sha”) Bugyi was born in Lestock as a member of Our Lady of Perpetual Help Parish She points to the faith and the example of her parents as the foundation for her own sense of community “It is those roots that matter so much,” she said describing the great example her parents provided as she was growing up If one more person came for a Christmas meal or a family meal That strong spirit of generosity and service is behind Bugyi’s interest in philanthropy and working with the community to make the world a better place to provide an opportunity for them to give and to help them to realize that when you give The blessings of serving at Preston Park II have reinforced that firm belief “The people I have met here do not realize how much they have given back to me,” says Bugyi Children and youth are another priority for Bugyi we can do so much more to give our kids solid roots.” everybody has challenges and hardships – it is what we do with that experience that matters And when you have a sense of community and of acceptance and belonging it comes easier.” Bugyi and her husband moved to New Zealand for a time before returning to Canada to live and work in Meadow Lake and then Humboldt she and her husband set up their own business selling it after nine years to move with their four children to Saskatoon in 2013 In her time at Preston Park II Bugyi says she has worked to “establish a community within a community.” Listening and compassion have been integral to her leadership at the retirement residence “We worked to create an experience that sets up everyone for success that lets each person know ‘there is a place for you here.’” In his message to the diocese about the appointment board Chair Ray Kolla looked ahead to the ongoing mission of the Diocese of Saskatoon Catholic Foundation as we continue the Catholic Foundation’s mission to provide the financial resources for current and future needs of our diocese through good stewardship practices fund-raising and helping parishioners to be actively engaged and supported through their faith journey,” Kolla said © 2022-2023 Roman Catholic Diocese of Saskatoon The Roman Catholic Diocese of Saskatoon does not necessarily endorse the content of any external sites linked from or listed on this website ReginaNewsThis Regina bakery is taking its cakes to a Food Network competitionBy Mackenzie ReadPublished: September 04, 2021 at 5:21PM EDT New York graduated 168 troopers today from the State Police Academy in Albany. “These new members of the New York State Police will serve one of the most prestigious and challenging roles in society as law enforcement personnel,” Gov. Andrew Cuomo said in a statement. The officers completed 26 weeks of training. They received a salary of $50,374 while in the academy and will get a base salary of $66,905 when they start in the job. For the first 10 weeks, they’ll participate in a field-training program. Here’s a list of the graduating troopers Thursday, their hometowns and where they will be stationed. A new option to help prevent food waste while saving money has arrived in Saskatchewan The phone app Too Good To Go allows businesses to sell food nearing its best before date — that would otherwise be wasted — at discounted prices the service launched in Canada in July 2021 More than 6,000 Canadian businesses are currently registered Subscribe now to read the latest news in your city and across Canada Create an account or sign in to continue with your reading experience baskets of surplus food are posted at around one third of the original price and customers can choose one in their location to pick up There are 40 businesses signed up so far in Saskatoon and 39 in Regina While the app officially launched in the province Thursday has already been using the service for the past week “I have one package right now three days a week not many of his customers would buy sale items at reduced prices was that he doesn’t have to market the service “They approached me and I thought that would be an awesome idea who owns Prairie Doughnut and Poutine in Saskatoon began using the app last week and also said it has been successful so far “I’ve had about 40 to 45 orders in the past five days,” he said By signing up you consent to receive the above newsletter from Postmedia Network Inc The next issue of Headline News will soon be in your inbox Interested in more newsletters? Browse here. “We always give fresh doughnuts to everyone if I made around 40 or 50 doughnuts but if somebody asked for just 12 doughnuts then what should I do with the remaining doughnuts?” Shah said he has given doughnuts away and donated them in the past “But sometimes it is hard to get some people to walk in here who want to try doughnuts,” he said People can order and come here and they can get whatever we have left.” Too Good To Go’s senior public relations manager said when it comes to the guidelines of the food that can be sold on the app “nothing that can’t be sold for full retail value can be sold on the app.” She said the app is not a way for companies to extend the life of the food “A really good example is pizza cannot be under those heat lamps for more than three hours so then it also cannot be in a Too Good To Go bag for that period either.” She added the bag might contain items that are on their best before date or one day past it if it was packed the night before Soteroff said new stores are frequently joining and the app could expand to smaller Saskatchewan markets in the future Their strategy is to start in more populated cities and move out from there “We don’t want to be in a super remote area where there is just one store because it makes for a bad consumer experience and a bad business experience,” Soteroff said App users can filter their search based on categories like produce baked goods or prepared meals and stores assign a time window for pickup participating businesses across the country have earned a combined $10 million on food that would otherwise go to waste There is a $2 operational fee the app takes from every transaction Food scraps, yard waste, pizza boxes, napkins and tissues can go into the carts, diverting around 50 per cent of the average household’s waste from the landfill. The carts will begin being delivered on Saturday. transmission or republication strictly prohibited This website uses cookies to personalize your content (including ads), and allows us to analyze our traffic. Read more about cookies here. By continuing to use our site, you agree to our Terms of Use and Privacy Policy You can manage saved articles in your account Enjoy some moments from the big day in form of some of the many pictures taken by Aleksi Sutinen Actin depolymerizing factors (ADFs) are among the key proteins involved in regulation of actin This study used various biochemical and structural biology methods to uncover several key aspects of ADFs from the malaria parasite and its vector The results show that ADFs from the malaria parasite can bind different phosphoinositides and the binding may be regulated by phosphorylation The parasite ADFs also bind to actin in a pH dependent manner This study also presents the three-dimensional structure of the ADF from the Anopheles mosquito The structure is different from that of the parasite ADFs The mosquito ADF interacts tightly with actin (https://www.oulu.fi/en/theses/structure-and-function-actin-depolymerizing-factors-malaria-parasite-and-its-vector) “Jesus calls each of us to be a beacon of light for the world, urging us not to turn away from others in an attitude of indifference, and allow our light to illumine and support others.” – Bishop Mark Hagemoen, 2024 BAA message The 2024 Bishop’s Annual Appeal is underway across the Roman Catholic Diocese of Saskatoon, with Bishop Mark Hagemoen recently announcing the 2024 theme, “On the Pilgrimage of Hope,” echoing the theme of the upcoming Jubilee 2025 celebrations announced by Pope Francis The goal for the 2024 Appeal is $1.4 million Initiated some 40 years ago by the late Bishop James Mahoney, today the Bishop’s Annual Appeal is an undertaking led by the Diocese of Saskatoon Catholic Foundation, the fund-raising arm for the diocese of Saskatoon “The Bishop’s Annual Appeal has a long history — even a legacy — in the diocese,” said Bishop Mark Hagemoen at a breakfast for parish leaders and BAA volunteers held Sept immediately before a day-long diocesan gathering to launch another ministry year there is this practical question of how do we resource the good work and the service we need to do,” the bishop said “The Bishop’s Annual Appeal is about the mission of the church – full stop,” Hagemoen added later at the Administration Day gathering as he reflected upon the diocesan Pastoral Plan priority to “Move from Maintenance to Mission.” “We all need to be engaged in the support of the mission.” and donors for their support for the Bishop’s Annual Appeal which funds outreach and faith formation across the diocese Diocese of Saskatoon Foundation Executive Director Raissa Bugyi provided an overview of this year’s Appeal including the release of a BAA video offering a glimpse into the impact of programs funded through the diocesan Appeal Executive Director of the Diocese of Saskatoon Catholic Foundation provided an overview of the 2024 Appeal at a morning meeting Sept During the Administration Day gathering of parish leaders later in the day Sept the bishop’s delegate for the Annual Appeal also spoke about its legacy and its importance he witnessed his parents place a priority on charitable giving and on supporting their parish even when money was tight — and he also recalled the first time they were asked to donate to something more — to the diocesan Appeal It was a call to join together with other Catholics across the diocese to offer ministry and outreach that a single parish probably could not do all on its own “It is a call to share in something bigger,” Cooper said also encouraging pastors and leaders to make their own gift to the Appeal first “We can’t ask for what we don’t give ourselves.” pastor and rector of the Cathedral of the Holy Family in Saskatoon spoke about the impact of the BAA in the diocese In his annual written and video message about the 2024 Appeal Bishop Hagemoen provided an overview of its impact in the diocese “Through the various ministries supported by the Bishop’s Annual Appeal the faithful of the Roman Catholic Diocese of Saskatoon bring hope to others,” he said teach catechism to our children and young people and provide pastoral care to the hospitalized and sick We also provide reconciliation and healing to those in prison We are supporting and training new priests and deacons to lead us fostering faith growth through youth and adult ministries We continue to invite lay adults into the Catholic faith as well as support and train adult leaders for ministry and service.” Hagemoen and Bugyi both expressed their gratitude to parishes and volunteers for their support of the Bishop’s Annual Appeal over the years Bishop’s BAA 2024 Message:   (PDF in English) / (PDF in French) More information and online giving:  dscf.ca/baa Kiply Lukan Yaworski is the communications coordinator for the Roman Catholic Diocese of Saskatoon – rcdos.ca Saskatoon Bishop Mark Hagemoen joined residents and guests at St 27 to celebrate the memorial of the saint who founded the Ursuline order of consecrated religious women and to dedicate a new mural highlighting the history and ministry of the Ursulines of Prelate The feast day celebration at St. Angela Merici Residence began with Mass concelebrated by Bishop Hagemoen and Fr Lector for the celebration of the Eucharist was Sr OSU; cantor was Director of Spiritual Care and Mission Judy Gatin; with music ministry led by Sr a mural describing the history and the ministry of the Ursulines of Prelate was dedicated The St. Angela Merici faith-based residence — established by the Ursulines in Saskatoon and now operated by Emmanuel Care — was named in honour of the saint who started the Company of St The Ursulines of Prelate were established in St Joseph’s Colony (now the western portion of the diocese of Saskatoon) more than 100 years ago beginning with three sisters from Germany – Mother Clementia Graffelder who came to establish schools at the invitation of Fr During the dedication celebration on the Feast of St Angela Merici Residence and the mural project of the Ursulines of Prelate at a dedication for a new “Educating For Life” mural about the history and ministry of the Ursulines of Prelate “This personal care home was originally built in 1986 as a retirement home for the Ursuline Sisters there have been many changes and some significant transformations.” the Sisters in residence were still quite active with some still engaged in volunteer ministry in the community “As years passed the needs of the resident Sisters were changing…until in 2000 we hired the first care aides more lay staff were added in all departments – care of the Sisters a Personal Care Home license was granted for the facility to operate according to Saskatchewan Health Authority Policies and receive residents other than Ursuline Sisters we acted on a decision our community had made in 2015 to transfer ownership and management of St Angela Merici Residence to Emmanuel Care – the Catholic Health Corporation of Saskatchewan 2020 – just before the pandemic was declared.” the Ursulines of Prelate began the process of finding homes for museum artifacts located in a Heritage Room in the building “It was decided to dismantle the Heritage Room and donate its contents to schools parishes and organizations that would use them to continue our mission of ‘Educating for Life.’  This was a major task and was completed just a few months ago,” said Lewans “In order to preserve our story and some of the more valuable artifacts of the Ursuline Sisters we asked and received permission from Anne Miller to create the display you see before you,” she explained effort and love” that the leadership team put into planning the display “The mural is a brief history of our Ursuline Congregation and the cabinet of artifacts contains tangible items that illustrate part of that history” she said “It certainly is appropriate for us to dedicate it today on the feast of St Angel Merici who is both the foundress of the Ursuline Order and also the patron saint of this residence Lewans also acknowledged the work of professional designer Deanna Miller who helped the OSU leadership team design the mural “Thank you very much Deanna; we could not have done this without you I also acknowledge the practical work of 77 Signs the company that printed and installed the mural and did the necessary carpentry work.” RELATED: Ursulines of Prelate celebrate their centennial in 2019 – ARTICLE RELATED: Ursulines of Bruno celebrate their centennial in 2013 – ARTICLE the content of any external sites linked from or listed on this website.