"For non-chemists, aromatic compounds are particularly stable cyclic molecular structures. They are the building blocks of many of our everyday products, such as dyes, fragrances, flavors and some cosmetic and pharmaceutical ingredients," explained Jérémie Blache, Pili's CEO adding that the company is the first to offer petroleum-free aromatic raw material on an industrial scale To sustainably produce a source of aromatic compounds Pili uses industrial fermentation based on its proprietary microorganisms Fermentation makes it possible to avoid fossil raw materials by using non-food sugars derived from biomass several tons of the 90% purity aniline derivative have already been produced at industrial grade which is already REACh registered and ready to be commercialized as an intermediate "Anthranilic acid is a very interesting platform molecule the chemistry accessible from this intermediate is wide," commented Guillaume Boissonnat-Wu "Pili is able to develop all or part of the synthesis pathways from this intermediate towards references of dyes all based on the principles of green chemistry." Pili plans to collaborate with partners interested in co-developing ingredients derived from aromatics chemistry and committed to decarbonizing their product portfolios CHEManager Spotlight is an exclusive event tailored for practitioners and decision-makers in the chemical industry This part of our event series delves into the latest trends and innovations in logistics to streamline your operations and drive efficiency ProteiNext is an annual symposium that offers a platform for sharing insights on protein analysis The Seahawks added depth to their defensive line on Monday who was waived by the Dolphins on Saturday who injured his ankle in Seattle's Week 9 game against the Rams was placed on injured reserve to make room on the 53-man roster appeared in 12 games over the past two seasons with the Dolphins earning All-Pac-12 honorable mention honors as a freshman Check out the Seahawks 53-man roster for the 2024 season This is a 2024 photo of Rayshawn Jenkins of the Seattle Seahawks NFL football team This image reflects the Seahawks active roster as of June 10 This is a 2023 photo of Michael Dickson of the Seattle Seahawks NFL football team This image reflects the Seattle Seahawks active roster as of June 5 This is a 2023 photo of Jason Myers of the Seattle Seahawks NFL football team This is a 2024 photo of Sam Howell of the Seattle Seahawks NFL football team This is a 2023 photo of Geno Smith of the Seattle Seahawks NFL football team This image reflects the Seattle Seahawks active roster as of June 5 This is a 2024 photo of Coby Bryant of the Seattle Seahawks NFL football team Seahawks cornerback/safety Coby Bryant (8) This is a 2024 photo of Kenneth Walker III of the Seattle Seahawks NFL football team Seahawks running back Kenneth Walker III (9) This is a 2023 photo of Uchenna Nwosu of the Seattle Seahawks NFL football team This is a 2024 photo of Jaxon Smith-Njigba of the Seattle Seahawks NFL football team Seahawks wide receiver Jaxon Smith-Njigba (11) This is a 2024 photo of DK Metcalf of the Seattle Seahawks NFL football team This is a 2024 photo of Tyler Lockett of the Seattle Seahawks NFL football team Seahawks wide receiver John Rhys Plumlee (17) practiced at the Virginia Mason Athletic Center on Thursday This is a 2024 photo of Jake Bobo of the Seattle Seahawks NFL football team Seahawks rookie receiver Jake Bobo makes his first touchdown catch on a pass from Geno Smith in the fourth quarter This is a 2024 photo of Julian Love of the Seattle Seahawks NFL football team This is a 2023 photo of Devon Witherspoon of the Seattle Seahawks NFL football team Seahawks cornerback Devon Witherspoon (21) This is a 2024 photo of Tre Brown of the Seattle Seahawks NFL football team This is a 2024 photo of Kenny McIntosh of the Seattle Seahawks NFL football team This is a 2024 photo of Zach Charbonnet of the Seattle Seahawks NFL football team Seahawks running back Zach Charbonnet (26) This is a 2024 photo of Riq Woolen of the Seattle Seahawks NFL 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football team This image reflects the Seahawks active roster as of June 6 This is a 2024 photo of AJ Barner of the Seattle Seahawks NFL football team This is a 2024 photo of Jarran Reed of the Seattle Seahawks NFL football team This is a 2024 photo of Byron Murphy II of the Seattle Seahawks NFL football team Seahawks defensive tackle Byron Murphy II (91) This is a 2024 photo of Mike Morris of the Seattle Seahawks NFL football team This is a 2024 photo of Johnathan Hankins of the Seattle Seahawks NFL football team Seahawks defensive tackle Johnathan Hankins (97) Seahawks defensive lineman Roy Robertson-Harris (98) This is a 2024 photo of Leonard Williams of the Seattle Seahawks NFL football team Seahawks defensive end Leonard Williams (99) The Seahawks waived four players on Tuesday which opens more roster spots ahead of signing undrafted free agents later this week The Seahawks waived nose tackle Cameron Young on Wednesday bringing back the quarterback who spent two seasons with the team from 2022-2023 Tight end Brady Russell signed his exclusive rights free agent tender on Wednesday The Seahawks signed receiver Steven Sims Tuesday adding receiver depth and a candidate to handle kick and punt returns The Seahawks signed veteran tight end Eric Saubert on Monday The Seahawks added to their secondary Monday adding safety and special teams standout D'Anthony Bell The Seahawks re-signed linebacker Josh Ross and added receiver River Cracraft on Friday The Seahawks signed cornerback Shemar Jean-Charles on Wednesday Cooper Kupp signed with the Seahawks Tuesday making official a return home for the Yakima native The Seahawks agreed to terms on a deal with former Rams standout Cooper Kupp a Yakima native who will be returning to his home state The Seahawks made a big splash early in free agency The Miami Dolphins announced they have waived defensive tackle Brandon Pili Pili appeared in 12 career games in two seasons with Miami (2023-24) He also spent time on the practice squad with the Dolphins in 2023 Pili entered the NFL as an undrafted college free agent with Miami in 2023 where he appeared in 52 career games with 74 tackles (28 solo) four passes defensed and one forced fumble He earned honorable mention All-Pac 12 honors as a true freshman in 2017 when he totaled 14 tackles (three solo) and blocked a field goal the Miami Dolphins and South Florida Motorsports (SFM) announced an expanded partnership with Verizon The Miami Dolphins announced today that they have signed linebacker Willie Gay Jr The Miami Dolphins announced today that they have re-signed defensive tackle Benito Jones The Miami Dolphins announced today that they have signed cornerback Artie Burns as an unrestricted free agent The Miami Dolphins announced today that they have signed tight end Pharaoh Brown as an unrestricted free agent The Miami Dolphins announced today that they have signed punter Ryan Stonehouse The Miami Dolphins announced today that they have signed quarterback Zach Wilson as an unrestricted free agent The Miami Dolphins announced today that they have signed linebacker K.J The Miami Dolphins announced today that they have signed running back Alexander Mattison as an unrestricted free agent The Miami Dolphins announced today that they have re-signed offensive lineman Liam Eichenberg The Miami Dolphins announced today that they have re-signed linebacker Tyrel Dodson The Miami Dolphins announced today that they have signed safety Ifeatu Melifonwu as an unrestricted free agent Metrics details This study developed a three-dimensional ultrastructural analysis application using serial block-face scanning electron microscopy (SBF-SEM) to investigate surgically acquired human skin tissues containing the arrector pili muscle as well as the embedding using a carbon-based conductive resin The results revealed dense nerve fiber networks branching from nearby nerve fiber bundles outside the muscle and running among muscle fibers the dense nerve network running through and along arrector pili muscle fibers rarely penetrates the connective tissues between smooth muscle fibers and epithelial cells no individual smooth muscle fibers formed adhesion structures with the epithelial cells of the hair follicle ending in the dermal extracellular matrix near the epithelial cells These results indicate the usefulness of this approach for three-dimensional ultrastructural analyses of human skin tissues comprising follicular units and revealing structural changes in skin tissues especially the arrector pili muscle and nerve fibers with hair follicular epithelium especially whether and how it contacts the hair remains unclear due to the difficulty of observing this complex three-dimensional (3D) organ in situ This study developed an application of the 3D ultrastructural analysis using SBF-SEM to examine surgically acquired human facial skin tissues containing vellus hairs and APM and to address the above questions The present results indicate that our 3D ultrastructural analysis of human skin tissues can help reveal the unexplored ultrastructural characteristics of the APM including their interactions with nerve fibers and the follicular epithelium Acquiring serial electron microscopic images from the human arrector pili muscle (APM) with serial block-face scanning electron microscopy (a) A light microscopy image of the dermis (De) The area labeled with a small rectangle is magnified in the inset and the areas indicated by medium and large rectangles are magnified in panels (b) and (c) (b) An electron microscopic image showing the middle region of the APM (arrowheads) (c) An electron microscopic image showing the area near the end of the APM (arrowheads) approaching the hair follicle (HF) epithelium The area indicated by a rectangle is magnified in (e) obtained from another slice of the serial images (e) A highly magnified image of the APM tip near the HF demonstrating that muscle fibers (arrowheads) extend close to the HF epithelium (f) A three-dimensional (3D) reconstruction of the dataset of serial images at high resolution acquired from the middle region of the APM (g) A 3D reconstruction of the dataset of serial images at high resolution acquired from the end of the APM Dense nerve fiber network in the middle of the arrector pili muscle (APM) b) Representative electron micrographs with segmentation depicting an APM bundle (blue) and nerve fibers (yellow) The area indicated by a rectangle (a) is magnified in (b) d) Three-dimensional (3D) reconstruction showing the APM fiber bundle (c yellow) demonstrate a dense nerve fiber network running throughout the muscle fiber bundle and connected to an adjacent thick nerve fiber (d (e–h) Serial electron microscopic images of the nerve fiber connecting the extrinsic nerve fiber and the nerve network inside the APM (f–h The number of each image in the serial image dataset is shown in the upper right corner A dense nerve fiber network penetrates and runs along arrector pili muscle (APM) fibers but rarely contacts the hair follicle epithelium (a) A representative electron micrograph with segmentation showing APM (blue) (b–e) Three-dimensional (3D) reconstructions of muscle and the epithelium from two different angles showing few contacts between nerve fibers and the hair follicule epithelium (b the nerve fiber network runs along the APM (d Interaction between the arrector pili muscle (APM) and connective tissues surrounding the hair follicle epithelium (a) A representative image of segmentation showing all individual APM fibers (arrows various colors) and the hair follicle epithelium (arrowheads (b) Three-dimensional (3D) reconstruction of individual APM fibers (arrows Note that each fiber appears to have a variable but non-negligible distance from the hair follicle epithelium (c–f) Serial images showing an individual muscle fiber (arrows h) 3D reconstruction showing only muscle fibers whose ends are included in the observed volume (various colors) and being close to the epithelium (arrowheads gray) is shown together with the closest distance between each fiber and the epithelium (h lines with blue spheres on the muscle side and orange spheres on the epithelium side) The muscle surface closest to the epithelial surface is not always at the end of the muscle (i) Graph illustrating the shortest distance between each muscle fiber and the epithelium This study applied 3D ultrastructural analysis using SBF-SEM to investigate the human skin tissue and the spatial distribution and interactions of APM and follicular epithelium at the ultrastructural level Our findings revealed that nerve fibers formed dense networks branching from nearby dermal nerve bundles and running among APM fibers with rare connective tissue penetration surrounding them APM fibers did not form adhesion structures with hair follicle epithelial cells but ended in the dermal extracellular matrix These results indicate the usefulness of this approach for the 3D ultrastructural analysis of human skin tissues and their follicular units and the potential for revealing changes in APM and nerve fibers associated with hair follicles in aging and diseases such as its observational nature and the unknown follicle cycle stage; thus this suggests a dynamic regulatory system in humans where physical connections may be formed during specific phases of the hair follicle cycle there may be differences in physical connections among species or body areas as the current study focused on the vellus hairs in human facial skin Future studies are warranted to address these possibilities and understand the interactions between nerve branches of the APM and the hair follicles A direct interaction may be more evident at early developmental stages than in older humans Understanding variations and/or changes in the interactions between APM and hair follicles may help understand hair pathology and develop effective solutions to hair diseases The ethics committees of the Shiseido Research Center the International University of Health and Welfare and the National Institute for Physiological Sciences approved this study (C02208 which was conducted in compliance with the ethical guidelines for life science and medical research involving human subjects was prepared from the surplus forehead skin excised during plastic surgery on a 20-year-old woman The sample was fixed by immersion in 2.5% glutaraldehyde and 2% paraformaldehyde in 0.1 M phosphate buffer over a few nights The sample was immersed in the fixative as soon after excision as possible and the time between excision and immersion was about 10 min the resin blocks were transferred onto rivets for imaging and ~ 1 μm thick sections from each block were transferred to glass slides for light microscopic observation with an optical microscope (BX-63; Olympus Japan Co.) The block containing the identified APM was trimmed and its surface was treated with gold sputtering to increase block conductivity The block was then imaged with a field-emission scanning electron microscope (Merlin with Gemini II column and field-emission electron gun) equipped with 3View Inc.) but not with focal charge compensation We acquired 561 serial EM images at a 6.5 nm/pixel resolution and 80 nm/slice thickness from the middle region of the APM and 1,014 serial EM images at a 7 nm/pixel resolution and 70 nm/slice thickness from the APM end interacting with the hair follicle which measures the minimal distance between the surfaces of object pairs All study data are included in the article Rittie, L. & Fisher, G. J. Natural and sun-induced aging of human skin. Cold Spring Harb Perspect. Med. 5, a015370. https://doi.org/10.1101/cshperspect.a015370 (2015) Kabashima, K., Honda, T., Ginhoux, F. & Egawa, G. The immunological anatomy of the skin. Nat. Rev. Immunol. 19, 19–30. https://doi.org/10.1038/s41577-018-0084-5 (2019) Ezure, T. Vellus hair condition is related to dermal elasticity and facial sagging severity. Skin. Res. Technol. 29, e13287. https://doi.org/10.1111/srt.13287 (2023) Torkamani, N., Rufaut, N. W., Jones, L. & Sinclair, R. D. Beyond goosebumps: does the arrector pili muscle have a role in hair loss? Int. J. Trichology 6, 88–94. https://doi.org/10.4103/0974-7753.139077 (2014) Halata, Z. Sensory innervation of the hairy skin (light- and electronmicroscopic study. J. Invest. Dermatol. 101, 75S–81S. https://doi.org/10.1111/1523-1747.ep12362877 (1993) Fujiwara, H. et al. The basement membrane of hair follicle stem cells is a muscle cell niche. Cell 144, 577–589. https://doi.org/10.1016/j.cell.2011.01.014 (2011) Ezure, T., Amano, S. & Matsuzaki, K. Quantitative characterization of 3D structure of vellus hair arrector pili muscles by micro CT. Skin. Res. Technol. 28, 689–694. https://doi.org/10.1111/srt.13168 (2022) Stander, S., Schmelz, M. & Skin Innervation J. Invest. Dermatol. 144, 1716–1723, doi:https://doi.org/10.1016/j.jid.2023.10.047 (2024) Zhang, B. & Chen, T. Local and systemic mechanisms that control the hair follicle stem cell niche. Nat. Rev. 25, 87–100. https://doi.org/10.1038/s41580-023-00662-3 (2024) Mizutani, Y. et al. Three-dimensional structure analysis of melanocytes and keratinocytes in senile lentigo. Microscopy (Oxf). 70, 224–231. https://doi.org/10.1093/jmicro/dfaa054 (2021) Ohno, N., Karube, F. & Fujiyama, F. Volume electron microscopy for genetically and molecularly defined neural circuits. Neurosci. Res. https://doi.org/10.1016/j.neures.2024.06.002 (2024) Bennett-Kennett, R. et al. Sensory neuron activation from topical treatments modulates the sensorial perception of human skin. PNAS Nexus. 2, pgad292. https://doi.org/10.1093/pnasnexus/pgad292 (2023) Glatte, P., Buchmann, S. J., Hijazi, M. M., Illigens, B. M. & Siepmann, T. Architecture of the cutaneous autonomic nervous system. Front. Neurol. 10, 970. https://doi.org/10.3389/fneur.2019.00970 (2019) Shwartz, Y. et al. Cell Types Promoting Goosebumps Form a Niche to Regulate Hair Follicle Stem Cells. Cell 182, 578–593 e519. https://doi.org/10.1016/j.cell.2020.06.031 (2020) Akiyama, M., Dale, B. A., Sun, T. T. & Holbrook, K. A. Characterization of hair follicle bulge in human fetal skin: the human fetal bulge is a pool of undifferentiated keratinocytes. J. Invest. Dermatol. 105, 844–850. https://doi.org/10.1111/1523-1747.ep12326649 (1995) Mitochondrial immobilization mediated by syntaphilin facilitates survival of demyelinated axons Conductive resins improve charging and resolution of acquired images in electron microscopic volume imaging TrakEM2 software for neural circuit reconstruction Microscopy Image Browser: a platform for segmentation and analysis of Multidimensional datasets Berger, D. R., Seung, H. S. & Lichtman, J. W. VAST (volume annotation and Segmentation Tool): efficient manual and semi-automatic labeling of large 3D image Stacks. Front. Neural Circuits. 12, 88. https://doi.org/10.3389/fncir.2018.00088 (2018) Urakubo, H., Bullmann, T., Kubota, Y., Oba, S. & Ishii, S. UNI-EM: an environment for deep neural network-based automated segmentation of neuronal Electron microscopic images. Sci. Rep. 9, 19413. https://doi.org/10.1038/s41598-019-55431-0 (2019) Tanaka, T. et al. Large-scale electron microscopic volume imaging of interfascicular oligodendrocytes in the mouse corpus callosum. Glia 69, 2488–2502. https://doi.org/10.1002/glia.24055 (2021) Download references Harumi Watanabe (Jichi Medical University) Mami Matsumoto (National Institute for Physiological Sciences) for their technical assistance This work was supported by collaborative research funding from Shiseido Co. Department of Plastic and Reconstructive Surgery International University of Health and Welfare National Institute for Physiological Sciences All authors reviewed and approved the manuscript The authors declare that this study was supported by the collaborative research funding from Shiseido Co. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Below is the link to the electronic supplementary material Download citation DOI: https://doi.org/10.1038/s41598-025-88615-y Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing newsletter — what matters in science « Back BYU FOOTBALL BY MITCH HARPER SALT LAKE CITY – Former BYU football linebacker Keenan Pili has suffered another season-ending injury with the Tennessee Volunteers Pili suffered an ACL injury on Saturday during Tennssee’s overtime win over Florida Tennessee head coach Josh Heupel announced during his weekly press conference that Pili would be out for the remainder of the season A post shared by Keenan Pili (@keenanpili7) “There’s nobody that represents Tennessee better than him,” Heupel said “He’s a great leader and will continue to be that inside of our building I’m heartbroken for him and his family A guy that absolutely does everything right It’s Pili’s third season-ending injury, dating back to an ACL injury he suffered in 2021 at BYU against Arizona State He also suffered a season-ending triceps injury in last year’s season-opener for the Vols against Virginia The various injuries to Pili caused him to have this year be his seventh in college football Pili was Tennessee’s team leader in tackles this year with 29 tackles The former Timpview High graduate transferred out of BYU after the 2022 season where he was a two-time captain for the Cougars and 3.5 sacks during four years on the field at BYU and two seasons at Tennessee Tennessee is currently 5-1 this season and ranked No. 11 in the latest AP Top 25 poll Mitch Harper is a BYU Insider for KSLsports.com and hosts the Cougar Tracks Podcast (SUBSCRIBE) and Cougar Sports Saturday (12–3 p.m.) on KSL Newsradio. Follow Mitch’s coverage of BYU in the Big 12 Conference on X: @Mitch_Harper Download the new and improved KSL Sports app from Utah’s sports leader. It allows you to stream live radio and video and stay up to date on all of your favorite teams. Follow @Mitch_Harper 2025Minnesota Lynx forward Alissa Pili (35) and her teammates walk off the court after the Lynx beat the Seattle Storm 83-70 on Tuesday 2024 at the Climate Pledge Arena in Seattle The standout Dimond High graduate played her first regular season game after being drafted in the first round by the Minnesota Lynx (Loren Holmes / ADN) The Alaska School Activities Association announced the Class of 2025 of the Alaska High School Hall of Fame on Friday Among the dozen individuals who will be inducted are former Dimond multisport star and current professional athlete Alissa Pili and Palmer women’s hockey trailblazer Kerry Weiland The ceremony honoring the class is at 2 p.m April 27 at the Special Olympics Alaska Jim Balamaci Training Center in Anchorage The Hall was established to recognize and permanently honor individuals who displayed “high ethical standards and integrity while achieving excellence in high school athletics and activities,” according to ASAA It also recognizes those who have made “exemplary contributions” to the prep sports and activities landscape on the Last Frontier Categories include students who participated in athletics as well as contributors who served as coaches Any former student must have graduated at least five years ago to be eligible for induction This year’s class marks the 20th in the hall’s history and features 10 athletes and two activities teachers/coaches Pili racked up a staggering 13 state championships during her time in high school including leading the Lynx volleyball team to four straight titles in volleyball and the basketball team to a pair of state titles The 2019 graduate went on to have a stellar collegiate career in basketball including being named Pac-12 Freshman of the Year at USC and later Pac-12 Player of the Year at the University of Utah She now plays professionally in the WNBA for the Minnesota Lynx after being drafted No [Playing at a new level, Anchorage’s Alissa Pili finds her footing and connects with fans] “Kerry was always respectful and quiet, but on the ice, she wasn’t afraid of anything,” childhood friend and fellow Alaska sports legend Scotty Gomez said in a statement. “She was the real deal.” Freddy Hamilton III helped make Craig High School a powerhouse basketball program during his four years. From 2001-2003, he spearheaded Panthers teams that dominated the Region V 3A level, finishing as state runner-ups in 2002 and state champions in 2003. Hamilton was named to the Region V 3A All-Tournament Team for three straight years and the State All-Tournament Team as a junior and senior. “Freddy was one of your first two-way players, an exceptional point guard/forward who made it very difficult for the opposing team to guard him,” former Craig assistant coach James Mackie said in a statement. Rachel Johnson was a three-sport standout at Nikiski High School, where her list of accomplishments included receiving multiple First-Team All-State honors in volleyball and basketball and shining as a goalkeeper in soccer. She continued to shine on the pitch in college at Highline College, where she set a school record with an 11-game shutout streak, and then at Washington State University, where she helped lead the Cougars to big wins against nationally ranked opponents. “Rachel’s leadership stands out as a primary reason for our fond memories,” her former coach Scott Anderson said in a statement. “She made us a team, not just a group of individuals.” “Luke’s leadership on the field was unparalleled; he always led from the front,” his coach Jake Doth said in a statement. “Tobin was a floor general who made everyone around him better,” Grace Christian coach Jason Boerger said in a statement. “His assists reflect his unselfishness and team-first mentality. He valued team success over individual accolades, and that’s what made him stand out.” “His relentless work ethic and focus distinguish him as a Hall of Fame athlete,” former coach Randy Magner said. Nathan Schwartzbauer was a four-sport standout at Colony whose list of accomplishments includes earning All-Conference and All-State honors in football, winning back-to-back state titles in track and field in the high jump and helping lead the hockey team to back-to-back state championships as well. “Nathan is one of the best athletes I’ve ever had the honor to coach,” his hockey coach Eric Troisi said in a statement. “His athletic ability, combined with his work ethic, helped make Colony High School one of the best hockey teams in the state.” Pauline Tufi was a five-sport star at West Anchorage High School, where she shined especially bright in softball, earning Gatorade Player of the Year honors for three straight years from 2011-2013. She also starred in hockey, volleyball, basketball and track and field before taking her talent to Louisiana Tech University to continue playing softball. “Pauline was not just an athlete; she was a game-changer. She brought leadership and determination to every team she joined,” West girls basketball coach Stanley Engel said in a statement. “Her impact extended beyond her athleticism — her heart, leadership, and work ethic made her a true pillar in our community.” Stan Harris was a stalwart teacher, mentor and leader in music education at Palmer High School whose contributions included music programs ranging from jazz bands, choirs and musical theater productions that consistently received top honors and statewide acclaim. “Stan’s ability to teach both band and choir at the highest levels is unmatched,” former Wasilla music teacher Sara Guhl said in a statement. “His kindness, flexibility, and guidance for students is unparalleled.” Nathan Vereide’s contributions in the activity of Drama, Debate, and Forensics (DDF) were legendary. He served as a coach at Whitestone School from 1997 to 2015. His teams amassed 31 overall school sweepstakes titles in the 1A/2A/3A categories, including just one of two ever undefeated debate seasons in 2011. “Nathan’s leadership on the team was unmatched,” fellow coach Shawn Brisco said in statement. “His dedication to both the success of his students and to improving the activity itself made him a key figure in the growth of DDF in Alaska.” [Correction: This story has been updated to correct the year associated with the class to be inducted in the Alaska High School Hall of Fame.] Josh Reed is a sports reporter for the Anchorage Daily News. He's a graduate of West High School and the University of North Carolina at Pembroke. Opens in new windowADVERTISEMENTAnchorage Daily News Advertise with us • Contact Us • RTI Team Tennessee inside linebacker Keenan Pili is out for the remainder of the season after tearing his ACL, Vols’ head coach Josh Heupel announced on Monday afternoon Pili suffered the injury on Florida’s opening drive during the Vols’ 23-17 overtime win over the Gators on Saturday night at Neyland Stadium The super senior linebacker went to the injury tent immediately and quickly went to the locker room afterwards He returned to the sideline in crutches with a large brace on is right knee for the rest of the game Despite missing almost the entirety of the Florida game Pili is still tied as Tennessee’s leading tackler with 29 tackles to this point in the season Injury luck has been brutal for Pili in his two seasons at Tennessee suffering a season-ending collarbone injury in the Vols’ 2023 season-opener against Virginia before suffering another injury halfway through the 2024 season “I’m not sure there’s a better representative of what a Volunteer is than Keenan Pili,” Tennessee head coach Josh Heupel said. “You talk about a selfless guy that cares about the people around him way more than he cares about himself Pili’s college career is now over barring NCAA surprisingly giving him more eligibility Jeremiah Telander will step into a starting spot besides fellow sophomore Arion Carter Junior linebacker Kalib Perry and redshirt freshman Jalen Smith will also see increased roles for the rest of the season Telander has played an abundance of snaps so far this season as Pili’s backup and has performed well in them recording 18 tackles to this point in the season Both Perry and Smith have played throughout the season in both blowout wins and certain defensive packages Tennessee has had linebackers further down its depth chart deal with injuries including walk-ons Ben Bolton and talented freshman Edwin Spillman that duo could provide important depth for Tennessee’s linebacker room the rest of the season The Vols return to the field on Saturday afternoon where they’ll face Alabama at 3:30 p.m Greg McElroy and Molly McGrath are on the call for ABC Δdocument.getElementById( "ak_js_1" ).setAttribute( "value" COLLEGE: Pili appeared in 52 career games (2017-22) PERSONAL: Played defensive end at Westview High in Portland after playing three seasons at Dimond High in Alaska Also participated in wrestling and as a thrower on the track & field team Majored in non-governmental organizations & social change The Seahawks made several roster moves ahead of their Week 18 games against the Los Angeles Rams Share on FacebookShare on X (formerly Twitter)Share on PinterestShare on LinkedInHONOLULU (HawaiiNewsNow) - A Laie woman who has been charged in connection to the death of her 11-year-old adopted daughter is requesting supervised release Sina Pili, 39, is charged with manslaughter, endangering the welfare of a minor, and persistent nonsupport in connection to the death of Azaeliyah Pili-Ah You. She has pleaded not guilty to all charges. In a new court filing, Pili’s legal team is asking for her supervised release, or for a judge to set bail on the terms that she would not be working with minors, and would be released to her husband. A hearing is set for April 9, and her trial is scheduled for May 12. Copyright 2025 Hawaii News Now. All rights reserved. ShopLas Vegas Aces 5:59 PM0:00Alissa Pili Visits Prairie Island Indian CommunityThe Lynx Basketball Academy hosted a clinic with Alissa Pili on August 5th at Prairie Island Indian Community for 30 youth basketball players Alissa coached youth through skills and drills and inspired them to keep playing the sport they love Play 0:58 If you are having difficulty accessing any content on this website The Seahawks waived defensive tackle Brandon Pili on Saturday six days after they claimed him off waivers from the Dolphins two days before they faced the Los Angeles Rams on Monday night The second-year defensive tackle from USC had appeared in the Dolphins' first eight games On the same day they announced they had waived Pili the Seahawks also placed former Dolphins center Connor Wiliams on the reserve/retired list Head coach Mike Macdonald said Friday that Williams' decision to retire was not related to the knee injury that cost him the end of the 2023 season with the Dolphins and that affected his free agency this offseason He declined to elaborate on what led to Williams' decision the Seahawks traded Jerome Baker to the Tennessee Titans for Ernest Jones IV in a swap of linebackers Baker had signed with Seattle in the offseason after being released by the Dolphins It should be noted that Baker didn't play in either of his first two games with Tennessee despite being active the Seahawks this year signed three former Dolphins players Seattle doesn't have any more former Miami players on its active roster the Dolphins still have the two players they picked up from the Seahawks in 2024 linebackers Jordyn Brooks and Tyrel Dodson Brooks has played every snap on defense so far this season while Dodson is ready for his Dolphins debut after being claimed off waivers this week Dodson sounds this week like a player extra motivated to show up the Seahawks for releasing him despite the fact he was their leading tackler while starting every game “Emotionally, I’m still going through ups and downs of it,” Dodson told reporters Thursday. "I’m pissed off and I’m going to probably stay pissed off until my career ends [One] man’s trash is another man’s treasure The content on this site is for entertainment and educational purposes only Betting and gambling content is intended for individuals 21+ and is based on individual commentators' opinions and not that of Sports Illustrated or its affiliates All picks and predictions are suggestions only and not a guarantee of success or profit If you or someone you know has a gambling problem crisis counseling and referral services can be accessed by calling 1-800-GAMBLER No. 10 Tennessee football plays No but the injured linebacker will serve as a team captain Pili suffered a season-ending ACL injury in the Vols' 23-17 overtime win over Florida last week He will present the team at the opening coin toss and sophomore Jeremiah Telander is expected to replace Pili at middle linebacker But Tennessee didn't list any new injury updates on the SEC student-athlete availability report Starting left tackle Lance Heard was listed probable on the injury report on Thursday and reserve running back Cam Seldon was questionable But they were not listed on the updated report indicating they're available to play today Here’s the SEC student-athlete availability report for Tennessee and Alabama players The SEC student-athlete availability report was introduced this season for conference games only The initial report is released on Wednesday night of an SEC game week with daily updates leading to a final report 90 minutes prior to kickoff of a Saturday game players are designated by their school as available schools must accurately designate players’ participation status If the school has knowledge that a player may not be able to participate in the upcoming game for any reason (injury Failure to do so will subject schools to potential penalties ranging from $25,000 for a first offense to $100,000 for a third and further offenses Adam Sparks is the Tennessee football beat reporter. Email adam.sparks@knoxnews.com. X, formerly known as Twitter@AdamSparks. Support strong local journalism by subscribing at knoxnews.com/subscribe Get the latest news and insight on SEC football by subscribing to the SEC Unfiltered newsletter, delivered straight to your inbox. Tennessee will have to play the rest of the 2024 football season without its defensive leader Seventh-year senior linebacker Keenan Pili tore his anterior cruciate ligament during the first five minutes of Saturday night's 23-17 overtime triumph over Florida David Paschall has covered sports at the Chattanooga Times Free Press since 1999 and wrote for the Chattanooga Free Press in the nine years before that David has mostly covered college athletics with a focus on the University of Georgia from 2000 to 2019 and the University of Tennessee since 2020 He grew up in Chattanooga and is a graduate of the Baylor School and Auburn University David has received multiple writing awards and has served as a Heisman Trophy voter since 2003 He has also worked in radio in Chattanooga and has been on the SEC Network's "Classic Rewind" series since 2014 This document may not be reprinted without the express written permission of Chattanooga Times Free Press Material from the Associated Press is Copyright © 2025 audio and/or video material shall not be published rewritten for broadcast or publication or redistributed directly or indirectly in any medium Neither these AP materials nor any portion thereof may be stored in a computer except for personal and noncommercial use The AP will not be held liable for any delays errors or omissions therefrom or in the transmission or delivery of all or any part thereof or for any damages arising from any of the foregoing Thanks for visiting The use of software that blocks ads hinders our ability to serve you the content you came here to enjoy We ask that you consider turning off your ad blocker so we can deliver you the best experience possible while you are here Tennessee linebacker Keenan Pili released a heartfelt statement of appreciation on Wednesday following his season-ending injury Pili, a team captain and UT's leading tackler, suffered a torn ACL in his right knee during the Vols' 23-17 overtime win over Florida especially because Pili suffered a season-ending triceps injury in the first game of 2023 The BYU transfer is a 26-year-old and seventh-year senior He had already utilized a medical redshirt in two previous seasons He and his wife are expected their first child in February Pili posted the message "BEAT BAMA" along with the following statement on X thank you for helping me become a better man on and off the field thank you for sacrificing for me to live my dream thank you for treating me as family and giving me a place I can always call home Your love and support has been felt during this time but thank you for a lifetime of memories I will cherish and never forget I thank my Father in Heaven for allowing me to play this game Keenan Pili suffered an apparent right leg injury in Tennessee football vs The seventh-year senior linebacker will not return against the Gators Pili reached toward his right leg after making a tackle on a short completion to Florida's Arlis Boardingham near midfield in the first quarter Pili returned to UT this season after suffering a season-ending injury in the 2023 season opener against Virginia Pili used the extra year of eligibility he had due to a season-ending knee injury in 2021 at BYU He transferred to Tennessee following the 2022 season Pili was the first player to reach Boardingham after he caught an 7-yard pass from quarterback Graham Mertz on third-and-11 requiring medical attention on the field and help leaving the field Jeremiah Telander enters for Keenan Pili vs FloridaSophomore linebacker Jeremiah Telander entered for Pili on the following Florida drive Telander has 13 tackles in five games with a pass breakup and a fumble recovery Keenan Pili stats for Tennessee footballPili is Tennessee's leading tackler this season with 28 tackles in five games RETURN: Why Keenan Pili decided to return to Tennessee football in 2024 after season-ending injury The veteran linebacker is an integral part of UT's highly ranked defense 2024Dimond quarterback Cayden Pili runs the ball during a 14-7 win over West during the state Division I football championship on Oct (Loren Holmes / ADN) After leading the Dimond High football team to its first Alaska state championship in 23 years junior Cayden Pili on Friday became just the second player in program history to be named Gatorade Alaska Football Player of the Year “I’m very grateful and honored to receive this award,” Pili said in a statement “It’s pretty cool being able to bring a GPOY award to our program and being only the second for football is pretty cool.” The dynamic dual-threat quarterback and tenacious defensive back joins an elite contingent of student-athletes to receive this prestigious honor — including his older sister who was a three-time recipient during her illustrious prep career at Dimond She went on to have a decorated collegiate basketball career was selected in the first round of the 2024 WNBA by the Minnesota Lynx and flew home to present her younger brother with the state championship trophy after the Lynx topped West for the Division I title “I’m glad I could join the elite club of athletes before me especially my sister who was three-time GPOY,” Pili stated ‘Congrats and welcome to the club lol,’ but I just want to thank God teammates and coaches for supporting me along the way.” The award not only recognizes a student-athlete for their incredible feats of athleticism on the gridiron it also acknowledges exceptional academic achievement and exemplary character displayed on and off the field Pili maintained a 3.29 GPA and volunteered locally building and donating beds for needy children in Alaska Pili was a dominant force on both sides of the ball for the Lynx this past fall but especially on offense where he compiled 2,694 yards of total offense last season in 11 games with 1,744 coming as passer and the other 950 coming as a runner He scored a combined 40 touchdowns: 18 through the air with his arm and a whopping 22 coming on the ground with his legs he was responsible for both of Dimond’s touchdowns which came in the first half of a low-scoring affair “We are all hugely excited at Cayden being named Gatorade Player of the Year,” Dimond head coach Brian Yim said in a statement Cayden had an excellent statistical season while [Dimond football caps off undefeated season with DI state championship win against West] Pili’s list of junior accomplishments also includes being named Alaska MaxPreps Player of the Year in late October, receiving First-Team All-Conference honors and being named Offensive Player Of The Year. Pili is the first Dimond recipient of the Gatorade award since Brian Haas in 1987. Opens in new windowADVERTISEMENTADVERTISEMENTADVERTISEMENTADVERTISEMENTMost read by subscribers1 Volume 14 - 2024 | https://doi.org/10.3389/fcimb.2024.1425624 This article is part of the Research TopicHost-Bacteria Interactions in Fish PathogensView all 8 articles Type IV pili (T4P) are versatile proteinaceous protrusions that mediate diverse bacterial processes we reported the presence of a unique Type IV class C pilus we sought to functionalize the role of Tad pili in the pathogenicity of A Through a comprehensive comparative genomics analysis of 170 A the conserved presence of the Tad operon in vAh isolates was confirmed suggesting its potential contribution to pathogenicity the entire Tad operon was knocked out from A hydrophila ML09-119 to elucidate its specific role in A The absence of the Tad operon did not affect growth kinetics but significantly reduced virulence in catfish fingerlings highlighting the essential role of the Tad operon during infection hydrophila ML09-119 was significantly decreased in the Tad operon deletant Absence of the Tad operon had no effect on sensitivity to other environmental stressors it was more sensitive to low pH conditions Scanning electron microscopy revealed that the Tad mutant had a rougher surface structure during log phase growth than the wildtype strain indicating the absence of Tad impacts the outer surface of vAh during cell division of which the biological consequences are unknown These findings highlight the role of Tad in vAh pathogenesis and biofilm formation signifying the importance of T4P in bacterial infections the precise functions of the T4P have not been investigated previously serves as a bridge that facilitates interaction between the pathogen and its host during infection This study aimed to delineate the function of Tad pili in vAh isolate ML09-119 We conducted an expanded comparative genomics analysis involving 170 A hydrophila genomes sourced from the National Center for Biotechnology Information (NCBI) confirming the consistent presence of the Tad gene cluster in vAh genomes We constructed a mutant strain with complete deletion of the Tad operon and investigated its contribution to virulence in catfish hydrophila physiology and response to environmental stress was determined These results revealed the significant role of Tad pili in the virulence of vAh isolate ML09-119 highlighting its crucial function in mediating pathogenic interactions The phylogenetic tree for 170 genomes was build out of a core of 1893 genes per genome The core has 1711702 AA-residues/bp per genome Table 1 Genome features for 170 A hydrophila ML09-119 Tad pilus operon and its component genes (A) GC content analysis of the Tad operon within the A The genome location of the Tad operon is indicated in the figure along with their predicted or known functions The Tad genes are color-coded based on their respective functions Figure 2 Phylogenetic tree based on the Aeromonas hydrophila core genome and availability of individual Tad operon genes across 170 A Isolation sources and geographical locations for each genome are included Navy blue color represents the presence of Tad operon genes Branch lengths were reduced to fit the image A. hydrophila strain ML09-119 (Tekedar et al., 2013) was used as a representative of the vAh clonal group impacting U.S. channel catfish aquaculture. The bacterial strains and plasmids used in this study are listed in Table 2 Escherichia coli strains CC118 λpir and BW19851 were used for cloning and conjugation purposes and suicide plasmid pMEG-375 was used to transfer gene mutations into A Brain heart infusion (BHI) agar and broth (Difco Luria–Bertani (LB) agar and broth (Difco) were used for E the following antibiotics and reagents (Sigma-Aldrich USA) were used in the given concentrations: colistin (12.5 μg/mL) Table 2 Bacterial strains and plasmids used in the present study hydrophila Tad operon mutant was designated as vAhΔtad Table 3 Primers used to generate and verify in-frame deletion of vAh Tad operon All fish experiments were conducted under a protocol approved by the Institutional Animal Care and Use Committee (IACUC) at Mississippi State University. Virulence of vAhΔtad was compared to A. hydrophila ML09-119 wild-type strain (vAh-WT) by immersion route of exposure as described previously (Abdelhamed et al., 2016) specific-pathogen-free (SPF) channel catfish fingerlings (13.54 ± 0.54 cm 15.32 ± 1.98 g) were stocked into twelve 40-liter flow-through tanks (14 fish/tank) and acclimated for a week Tanks were assigned randomly to three treatment groups: vAhΔtad Fish were fed twice daily with a commercial catfish feed and water temperature was maintained at 30°C (±2) throughout the experiments the water levels in each tank were decreased to 5 L and 100 mL of overnight culture were added directly to each tank (1.9 × 109 CFU/mL water) Negative control (sham-infected group) tanks were treated similarly but exposed to 100 mL of sterile BHI broth Fish mortalities were recorded daily for 7 days and cumulative percent mortality calculated for each group Growth kinetics of vAhΔtad and vAh-WT in BHI broth were compared bacterial growth curves were generated by measuring the optical density at 600nm (OD600) on a multimode reader (BioTek Cytation 5) every half an hour for 72 h at 30°C The growth assays were conducted in two independent experiments each with 24 replicates for vAhΔtad and vAh-WT strains Biofilm formation of the vAhΔtad and vAh-WT strains were compared using a microtiter plate assay Five different colonies of each strain were cultured to log-phase (OD600 = 0.6 ± 0.05) at 28°C in BHI broth and diluted to OD600 of 0.1 using freshly prepared media A 100 µL of the diluted cultures were added into a 96-well plate and incubated at 28°C overnight Sterile BHI media (100 µL) was added to the blank control wells 8 replicate wells were used for each treatment and the control group the bacteria were discarded by turning the plate over and shaking out the liquid then gently submerging the plate in a tub of water The washing step was repeated twice to remove any unbound bacteria and media before staining 125 µL of crystal violet (CV) (0.1%) was added and the plate was incubated at room temperature for 15 min the microtiter plate was rinsed 3-4 times by submerging in sterile water as described above followed by vigorous blotting on a stack of paper to remove any unbound bacteria and dye The plate was air-dried and 125 µL of 95% ethanol was added to each well to solubilize the CV The plate was incubated for 15 min and the solubilized CV was transferred to a new flat bottom microtiter plate and absorbance was measured at 595nm The biofilm assay was conducted with 35 biological replicates for both vAhΔtad and vAh-WT strains The vAhΔtad and vAh-WT strains were cultured to log-phase (OD600 = 0.6 ± 0.05) at 28°C in BHI broth Two microliters of bacterial culture were spotted on a 0.6% BHI semi-solid plate and incubated at 28°C Replicate plates were used for statistical analysis The diameters of bacterial growth were measured after 16 hours of incubation Each motility assay was conducted with 7 independent biological replicates with 3 technical replicates per biological replicate for both the vAhΔtad and vAh-WT strains The ability of the vAhΔtad and vAh-WT strains to withstand extreme temperatures was evaluated Both strains were cultured to log phase (OD600 = 0.6 ± 0.05) at 28°C and then adjusted to an OD600 of 0.1 using sterile BHI broth The bacterial suspension was subsequently incubated at 18°C the cultures were washed twice with sterile phosphate-buffered saline (PBS) and spread on BHI agar plates for colony counting and statistical analysis The temperature assay was conducted with 4 biological replicates for both vAhΔtad and vAh-WT strains Viable bacteria were quantified by spreading on BHI agar plates after serially diluting The two strains’ percent viability (CFUs/mL) was calculated and compared The acid-alkali stress assay was conducted with 3 biological replicates for both vAhΔtad and vAh-WT strains Under optimal growth conditions at 28°C the vAhΔtad and vAh-WT strains were cultured to log-phase with an optical density of 0.6 ± 0.05 at 600nm and resuspended in fresh BHI broth containing 0.5 M sodium chloride (NaCl) 200 μL of each bacterial suspension was dispensed into separate wells of a 96-well microplate 200 μL of sterile BHI broth was added Growth was monitored every 1 h for 16 h at 28°C in an automatic microplate reader using absorbance at 600nm The osmotic stress assay was conducted with four biological replicates for both vAhΔtad and vAh-WT strains To evaluate the viability of the strains against environmental oxidants both vAhΔtad and vAh-WT strains were cultured to log-phase (OD600 = 0.6 ± 0.05) at 28°C in BHI broth Bacterial pellets were collected and washed twice with sterile PBS solution before treatment with 0.1M hydrogen peroxide (H2O2) Bacteria were incubated for 20 min at 28°C and the viable bacteria were quantified by spreading them onto BHI agar The percentage viability of the two strains in the treatment group (H2O2) compared to the control group (PBS) was calculated and compared between the mutant and wild-type strain The oxidative stress assay was conducted with four biological replicates for both vAhΔtad and vAh-WT strains The effects of Tad pili on the membrane structure of A hydrophila was evaluated using scanning electron microscopy Both the vAhΔtad and vAh-WT strains were grown at 30°C Pellets were collected from log phase and overnight cultures and bacteria were fixed in 2.5% glutaraldehyde and 2% paraformaldehyde in 0.1M sodium cacodylate buffer for 2 hours at room temperature prior to fixation in 1% osmium tetroxide for one hour Fixed pellets were then dehydrated in a graded ethanol series (10% Images were captured using a JEOL 6500F field emission SEM operating at 10KeV at the Mississippi State University Institute for Imaging and Analytical Technologies Mean cumulative percent mortality data were arcsine transformed and analysis of variance (ANOVA) was applied using PROC GLM in SAS for Windows v9.4 (SAS Institute the colony forming units/mL between the vAhΔtad and vAh-WT strains were compared and analyzed using one-way analysis of variance (ANOVA) An alpha level of 0.05 was used in all analyses a phylogenetic tree was constructed for the 170 A hydrophila strains based on a core set of 1893 genes per genome The core genome contained 1,711,702 amino acid residues per base pair or 290,989,340 in total the vAh isolates formed a distinct cluster with 100% branch conservation and this separation was verified by ANI and average amino acid identity (AAI) values (data not shown here) confirmed deletion of the Tad operon and the absence of additional random mutations outside the targeted locus Figure 3 Evaluating the role of Tad operon in A The vAhΔtad and vAh-WT strains were compared and analyzed using one-way analysis of variance (ANOVA) An alpha level of 0.05 was used for all analyses with asterisks (*) indicating statistical significance (A) Percent mortalities of fish immersion challenge and percent mortalities was calculated for each treatment Bacterial curves were generated by measuring the optical density at 600nm (OD600) on a multimode reader every half an hour for 72h at 30°C The strains were cultured to log phase and adjusted to OD600 of 0.1 The cultures were added to 96-well plate and incubated at 28°C for 24h The cultures were washed and air-dried post staining with crystal violet (CV) for 15min at room temperature The CV was solubilized with 95% ethanol for 15min Two microliters of the culture were spotted on 0.6% BHI semi-solid plates and incubated at 28°C for 24h The motility diameter for both strains were measured in millimeters these results indicate an impact of Tad pili on acid tolerance in vAh strain ML09-119 Figure 4 Characterization of vAhΔtad under different stress conditions vAhΔtad and vAh-WT strains were compared and analyzed using one-way analysis of variance (ANOVA) The strains were cultured to log phase and OD600 was adjusted to 0.1 with BHI medium The bacterial suspension was incubated at 18°C 28°C (as a control) and 37°C for 24h The cultures were double diluted with sterile PBS and plated onto BHI agar for colony counting and statistical analysis The strains were cultured at log-phase and the pellet was collected and subjected to treatment in BHI at pH 4.0 Viable cells were counted and compared for both strains by plating on BHI agar after serial dilutions The strains were cultured to log phase; pellets were collected and resuspended in BHI with 0.5M NaCl to adjust the OD600 of 0.1 The OD600 was monitored every 1h for 16h at 28°C The strains were cultured to log phase; pellets were collected and treated for 20min in PBS with 0.1M H2O2 and PBS without H2O2 as a control Viable cells were counted on BHI plates after incubating for 24h Figure 5 Ultrastructural observation of vAhΔtad and vAh-WT Bacterial strains were grown to log-phase in BHI at 30°C and specimens were examined with JEOL 6500F field emission SEM operating at 10 KeV (A) Cell structure of vAhΔtad at 18,000x and (B) at 55,000x under SEM (C) Cell structure of vAh-WT at 18,000x and (D) at 55,000x under SEM Amidst the dynamics of host-pathogen interactions pathogens are driven to persistently evolve and evade the host’s immune defenses These adaptations often occur by acquiring unique genes and systems through horizontal gene transfer which leaves a distinct imprint on the pathogen’s genetic makeup Comparative genomics allows identification of these unique genetic adaptations which can be specific for a bacterial species or for a genetic lineage within a species hydrophila is a genetically diverse species that has many unique lineages with varied environmental adaptations These analyses also support the notion that U.S and Chinese vAh isolates originated from the same monophyletic clade in silico analysis confirmed previous findings that vAh isolates consistently carry the Tad operon In vAh ML09-119, a set of 13 Tad genes organized within an operon structure were identified (Figure 1). The function of Tad in vAh was investigated through the generation of a knockout mutant that encompassed the entire operon. Compared to the wild-type strain, there was significantly reduced mortality in catfish fingerlings exposed to vAhΔtad indicating the Tad operon plays a critical role in the virulence of A. hydrophila ML09-119 (Figure 3A) This finding is consistent with the role of the Tad operon in diverse host-pathogen systems hydrophila isolated from human cases remains a subject for future investigation It is thought the Tad operon may provide a competitive edge by facilitating niche adaptation allowing the pathogen to persist in diverse environments In A. hydrophila ML09-119, the Tad operon has higher GC content (64.82%) than the average for the rest of the genome (60.82%) (Figure 1) suggesting acquisition from horizontal transfer vAh isolates consistently encode the Tad operon while exhibiting the absence of any type III secretion system (T3SS) (data not shown here) This suggests the mechanism of pathogenesis for vAh particularly how vAh manipulates function of host cells is quite different from pathogens that utilize T3SS to inject effector proteins directly into host cells and Tad may be an important adaptation in the absence of T3SS Tad pili may function as a sensor regulator for low pH inducing expression of other adaptive mechanisms or it may directly mediate resistance to low pH It is possible the bacterial surface alterations in vAhΔtad impacted host cell adhesion and/or induction of virulence mechanisms through signaling pathways resulting in reduced virulence these investigations into the function of Tad pili in vAh isolate A hydrophila ML09-119 provides valuable insights into its role in virulence and environmental adaptation Comparative genomic analysis confirmed the consistent presence of the Tad operon in the vAh subclade of A suggesting Tad is essential for vAh virulence in catfish biofilm formation and resistance to acid stress This work highlights the multifaceted role of Tad pili in A The presence of the Tad operon in some human A hydrophila isolates suggests it may contribute to pathogenesis in humans as well these findings demonstrate a clear role of Tad pili in contributing to virulence of vAh in catfish which is one of the most important diseases affecting catfish aquaculture in the southeastern United States Further investigation is warranted to delineate the role of Tad pili in vAh pathogenesis and explore its potential for treatment and prevention of motile Aeromonas septicemia The original contributions presented in the study are included in the article/Supplementary Material Further inquiries can be directed to the corresponding author The animal study was approved by Institutional Animal Care and Use Committee of the Mississippi State University The study was conducted in accordance with the local legislation and institutional requirements The author(s) declare financial support was received for the research This work was supported by the Mississippi State University College of Veterinary Medicine and the USDA Agricultural Research Service project 310048-182020-021000-371060 The authors thank the Laboratory Animal Resources and Care (LARAC) at the College of Veterinary Medicine for providing the SPF channel catfish and Dr Rivers for his assistance with the SEM imaging The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2024.1425624/full#supplementary-material Identification of protein secretion systems in bacterial genomes using macSyFinder version 2 MacSyFinder: A program to mine genomes 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Lawrence ML (2024) Tad pili contribute to the virulence and biofilm formation of virulent Aeromonas hydrophila Received: 30 April 2024; Accepted: 01 July 2024;Published: 31 July 2024 Copyright © 2024 Tekedar, Patel, Blom, Griffin, Waldbieser, Kumru, Abdelhamed, Dharan, Hanson and Lawrence. 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Josh Heupel didn’t provide an answer for who will be taking over Keenan Pili’s “green dot” communication helmet in the wake of Pili’s season-ending injury against Florida Heupel was non-committal in Tennessee’s choice because the Vols have not quite figured that out haven’t finalized that,” Heupel said on Monday as the Vols began the new week of practices Could be (Jeremiah) Telander that takes that one.” The NCAA passed a rule last spring allowing teams to have one player on offense and one on special teams wear a communication helmet labeled with a green dot on the back of the helmet Coaches can communicate with players before the play coach-to-player communication is cut off with either 15 seconds left on the play clock or the snap of the ball Tennessee veteran Keenan Pili was the Vols’ designated defensive helmet communicator throughout the season but suffered a season-ending ACL injury against Florida this past weekend Pili has been productive for Tennessee and is currently tied as the Vols’ leading tackler despite barely playing against Florida (29 total tackles) Pili and fellow starting linebacker Arion Carter combined for 62% of Tennessee’s linebacker snaps before the Florida game With Pili’s departure early in the game on Saturday Tennessee turned to linebacker Jeremiah Telander for a bulk of Pili’s work Telander averaged 17 snaps per game against Oklahoma and Arkansas but more than tripled that workload against Florida in Pili’s absence (67 snaps) Carter and Telander combined for 69 percent of Tennessee’s linebacker snaps against Florida It’s no surprise that Tennessee’s coaching staff is looking at one of those two players to be the next helmet communicator Tennessee linebacker Kalib Perry also saw a significant increase in workload against the Gators with Pili out Perry averaged just 8.5 snaps per game against Oklahoma and Arkansas but saw 35 snaps against the Gators on Saturday Tennessee linebacker Jalen Smith also saw a higher snap count against Florida but not as much of an increase as Telander and Perry Here’s a look at the snap count for all five Tennessee linebackers through the first six games of the season, courtesy of Pro Football Focus: 7 Alabama on Saturday afternoon at 3:30 p.m Check out Josh Heupel’s full Monday press conference below: The action never stops for the Seattle Seahawks even on a Monday following the biggest win of the year The Seahawks made a pair of roster moves on Monday afternoon and signed nose tackle Brandon Pili back to the practice squad Mabry has been in the Seahawks organization ever since he was signed as an undrafted free agent in the 2020 offseason Mabry has floated between the practice squad active roster and waivers for pretty much his entire career to this point He was elevated to the active 53-man roster prior to the Week 11 game against the 49ers due to injuries at the position to Noah Fant and Brady Russell and his only NFL stats came in 2022 when he caught one pass for seven yards for a touchdown Brandon Pili was claimed on waivers himself last week from the Miami Dolphins Pili has four career total tackles in the NFL NFC West Watch: Jaguars hire former Rams scouting director as GM Seahawks select star TE in mock 2025 draft Seahawks waive 2 players from the practice squad 3 RTs Seahawks may target at No. 50 overall in 2025 NFL draft Tim Banks watched how Keenan Pili handled himself for the past year The Tennessee football defensive coordinator was proud then as the linebacker worked through an injury in his first season with the Vols He’s even more proud now watching Pili fly around the field for the Vols “For him to finally see the fruits of his efforts I feel like a proud dad to finally see him go out there and do what he did,” Banks said Tuesday came back to Tennessee after his injury in UT’s 2023 season opener He’s the veteran presence in the middle of a menacing defense for the No who host Kent State (0-2) on Saturday (7:45 p.m Pili transferred to Tennessee from BYU prior to the 2023 season He suffered a season-ending arm injury in his first game The 26-year-old linebacker opted to return to Tennessee for another season Banks said Pili worked his tail off while being injury “He continued to push guys and continued to learn our defense.”  240-pound Pili has six tackles through two games He made two in the season opener against Chattanooga before having four against NC State PILI: Why Keenan Pili decided to return to Tennessee football in 2024 after season-ending injury Pili was at BYU for five seasons before transferring to UT He redshirted as a freshman in 2016 before he served a two-year mission for The Church of Jesus Christ of Latter-day Saints in Orlando but suffered a torn ACL early in the 2021 season which afforded him another season Arion Carter are Tennessee’s inside linebackersSophomore Arion Carter is Pili’s partner at inside linebacker for the Vols The former high school running back has made six tackles with 1.5 tackles for loss and a pass breakup Banks lauded Carter’s improving football IQ as he moved to linebacker “To see the growth that he has at linebacker has been amazing for me,” Banks said He’s always played hard but him being able to play a step quicker because he understands how offenses are trying to attack us." 234-pound Carter made 17 tackles in eight games as a freshman in 2023 Carter was a four-star recruit in the 2023 class out of Smyrna High School He was committed to Middle Tennessee initially as a running back before opening up his recruitment and landing at Tennessee “Tennessee losing a huge veteran presence on the defensive side,” ESPN sideline reporter Kris Budden said “I’ve just been told Keenan Pili is out for the remainder of the game with a lower-body injury I am watching him walk to the locker room right now under his own power It’s not good news for Tennessee on the defensive side.” CLICK HERE to go to PrizePicks and use code ON3 to receive a guaranteed $50 once you play $5 in lineups! Pili is one of the most experienced players in all of college football, originally being a member of the 2016 recruiting class. Five seasons were spent with the BYU Cougars before entering the NCAA transfer portal and landing with Tennessee. The linebacker is now in his second season with the Volunteers, his seventh overall. Not many programs have a player with that much time on the field they are able to rely on. Tennessee’s defense has been one of the best this season and Pili is a huge reason why. Not having him out there against Florida is going to be a huge blow. Top 101Shane BeamerPuts NCAA on blast Hot3Trump By clicking "Subscribe to Newsletter", I agree to On3's Privacy Notice, Terms and use of my personal information described therein Save $30 on your first month of Fubo by CLICKING HERE NOW! you can get your first month of Fubo for as low as $49.99 NBC and 200+ top channels of live TV and sports without cable Tennessee was nearly unrivaled in offensive production through the first three weeks of the season Averaging 63 points against non-conference opponents, that scoring total has been cut in half and then some since the Vols started SEC play. Held to 25 points against Oklahoma in a conference-opening win, Josh Heupel’s squad scored just 14 points during the loss to Arkansas in Week 6 Ahead of their matchup against Florida at home, Tim Tebow attempted to get a pulse on the Tennessee offense. He looked toward the stat sheet for evidence that the Vols have fallen off track since SEC play began. “This team started so hot,” Tebow said during an appearance on SportsCenter on Saturday morning ahead of SEC Nation. “Non-conference, they were dominating. 639 yards per game, but in-conference, yeah, you cut that by 300. That’s not the same team that’s been playing the last few weeks, and they’re just not on the same page.” Hot3Trump, Saban blastedNIL order ripped On3 is a registered trademark of On3 Media (Alika Jenner | AP) Minnesota Lynx forward Alissa Pili (35) in action during a WNBA basketball game Former Ute and now Minnesota Lynx forward Alissa Pili has signed an endorsement deal with Nike’s N7 brand N7 has supported Native American and Indigenous athletes through various Nike brand initiatives and a descendant of the Inupiat tribe in Alaska Pili will join famous Nike athletes like Olympic hammer thrower Janee’ Kassanavoid and Dallas Mavericks star point guard Kyrie Irving whose mother was a member of the Standing Rock Sioux Tribe and is currently playing in the 2024 NBA Finals against the Boston Celtics “I’m so honored to join the Nike Family as an N7 athlete,” Pili said in a statement. “Representation is important to me, and I look forward to making an impact in the league and demonstrating to all the Indigenous and Polynesian girls who love the game that they can reach their dreams.” Pili’s journey started off in Alaska, where she lived in the small town of Barrow (now called Utqiagvik) before committing and playing at USC for three seasons. For her final two seasons, the forward transferred to the Utes in Salt Lake City in search of more playing time. In 2023-24, Pili’s final season of collegiate eligibility, she averaged 21.4 points and 6.6 rebounds per game, while shooting 55% from the field. Pili’s performance landed her Second Team All-American honors from The Athletic and guided Utah women’s basketball to the second round of the NCAA Tournament. After finishing her career with the Utes, Pili was selected No. 8 overall by the Lynx. In her rookie season she has continued being an advocate both on and off the court for Indigenous athletes and communities. Following Minnesota’s contest vs. the Phoenix Mercury on June 7, Pili connected and met with various Indigenous leaders in Arizona. She also wore a commemorative tribal dress in honor of her Polynesian roots at the 2024 WNBA draft. Through the N7 initiative, Nike says it has helped donate $11 million to nearly 300 organizations over the past 15 years. Pili will look to extend that legacy by continuing to build her own brand on the Lynx and in the WNBA. For e-edition questions or comments, contact customer support 801-237-2900 or email subscribe@sltrib.com sltrib.com © 1996-2025 The Salt Lake Tribune ShareSaveCommentBETAThis is a BETA experience. opt-out hereLifestyleStyle & BeautyWhy Versatile Pili Oil Deserves A Place In Your Skincare RoutineByCarol RH Malasig Forbes contributors publish independent expert analyses and insights Carol RH Malasig is an expat journalist covering fashion and beauty.Follow AuthorJul 30 02:37pm EDTShareSaveCommentFrom its nuts as superfood to its oil as a do-it-all ingredient for skincare Long used as a natural remedy for skin concerns in the Philippines specifically in the central region of Bicol Pili oil is one of the skincare community’s best-kept secrets locals have harnessed the power of Pili oil to address a myriad of skin concerns from dryness and irritation to signs of aging this time-honored remedy is stepping into the spotlight gradually capturing the attention of beauty enthusiasts worldwide Much like the nutrient-rich Pili nut that’s been granted superfood status Comparable to olive oil with its high concentration of Vitamin E it’s particularly effective in promoting skin repair and protecting against environmental stressors Unlike many oils that sit on the skin’s surface delivering intense hydration and leaving skin feeling supple and nourished More after a botanical illustration from Hermann Adolph Koehler's Medicinal Plants (Photo by: Florilegius/Universal Images Group via Getty Images) The way Rosalina Tan’s face lights up when talking about the benefits of Pili oil is beautiful and her enthusiasm is infectious. Now in her 70s, she’s been an advocate of organic farming for most of her life, leading her and her daughter into putting up Pili Ani “Many people call the coconut tree the tree of life but I’d argue that the Pili tree is the same noting the many uses one can get out of the tree “Bicol is a region known for its typhoons and it’s interesting how Arguably the most established Filipino skincare brand in the international market Pili Ani’s mission is to make Pili oil mainstream showcases the versatility and potency of Pili oil earning rave reviews from users who appreciate both the efficacy and the ethical sourcing of the ingredients from local organic farmers Pili Ani's Self-care Night Recovery Oil soothes the Self-Care Night Recovery oil uses high concentration Pili oil The brand claims that it can help minimize breakouts and redness while brightening and moisturizing the skin The brand has since branched out into creating a Deep Detox Volcanic Exfoliating Mask from the ashes produced by Bicol’s Mayon Volcano and worked with influencer Bretman Rock in promoting the products to a wider audience Isarog takes Pili oil a step further by blending it with other indigenous Philippine ingredients symbolizing the strength and resilience of the local flora such as combining Pili oil with Yerba Buena (Philippine Mint) or Turnip (Singkamas) offer a fresh take on traditional skincare The result is a line of products that not only address common skin issues but also celebrate the rich natural heritage of the Philippines I already knew of Pili oil while growing up but it’s when I was diagnosed with chronic fatigue and I was recovering in Bicol that I started exploring the idea for Isarog.” At that time none of the products in the market worked for Odiamar From her interest in Pili and local botanics and work with the right people to create her own line “It was also timely that we were developing a property in Bicol for a resort and I wanted to craft an experience or product that is very Bicolano through these products that can be used there as well.” Isarog's Pili Night Rescue Lotion repairs and restores the skin Gaining popularity is Isarog’s Pili Night Rescue Lotion that’s been getting rave reviews from its loyal users Odiamar has also developed a line of soaps in sustainable and eco-friendly packaging The soaps come in scents that evoke memories from the Philippines’ natural environment each product is my way of honoring our Filipino heritage,” Odiamar added Moót is the rootword for love in the Bicol region’s language I’ve been wanting to champion Pili and have been studying it extensively since,” she admitted I attended an industry convention where the Philippines’ forgotten plants were discussed which made me want to incorporate those into our products.” Cruz is advocating for the use of Pili as a unique Through her research that’s now set to be published Cruz has found a way to optimize the extraction of Pili oil for use in beauty products and fragrances My research will be public as I want to share the method with other brands that create beauty products using Pili so that more people can know about this oil.” Love Moót's Body Oil moisturizes the skin using Pili oil and a blend of Philippine scents Volume 10 - 2022 | https://doi.org/10.3389/fphy.2022.875687 This article is part of the Research TopicActive Matter in Complex EnvironmentsView all 12 articles Work generated by self-propelled bacteria can be harnessed with the help of microdevices may exhibit unidirectional rotational or translational motion Swimming bacteria that propel with the help of actively rotating flagella are a prototypical example of active agents that can power such microdevices we propose a computational model of a micron-sized turbine powered by bacteria that rely on active type IV pili appendages for surface-associated motility We find that the turbine can rotate persistently over a time scale that significantly exceeds the characteristic times of the single cell motility The persistent rotation is explained by the collective dynamics of multiple pili of groups of cells attaching to and pulling on turbine we show that the turbine can rotate permanently in the same direction by altering the pili binding to the turbine surface in an asymmetric fashion We thus can show that by changing the adhesive properties of the turbine while keeping its symmetric geometry we can still break the symmetry of its rotation this study widely expands the range of bacteria that can be used to power nanofabricated microdevices due to high pili forces generated by pili retraction promises to push the harnessed work by several orders of magnitude Overview of the computational model of bacteria driving a rotatable turbine by attachment of type IV pili (A) Sketch of how pili binding and retraction can lead to a force acting on a cell Cells are located in a box together with the rotatable turbine The cells possess pili which they use for the motility on the substrate and for attachment to the turbine The turbine center is located at r(turb) and has an orientation defined by the angle α (C) Summary of all forces acting on the cells and turbine Friction forces with the environment (surrounding liquid and substrate) are not shown we are asking if cells with type IV pili that can create so large forces might be more attractive candidates to power microdevices we study the dynamics of a rotatable turbine immersed in a bath of twitching bacteria We investigate how the turbine rotation is affected by the binding and unbinding of the bacterial pili due to the adhesion of multiple pili to the turbine over time scales that can strongly exceed the characteristic time scales of the individual pili attachment the turbine can persistently rotate in one direction over extensive durations While these persistent rotations have a limited lifetime one can engineer a system where they become permanently unidirectional by introducing asymmetric binding of the pili to the turbine First, we introduce the computational model of twitching bacteria and their interactions with a turbine. A related version of this computational model was considered previously to describe bacterial surface motility [19] and bacterial aggregates [23–25] This also enables us to only consider a simpler two-dimensional system The experimental system we are mimicking are bacterial cells confined in a box with the turbine at its center (see Figure 1B) The cells can move over the substrate via pili binding and unbinding and additionally interact with the turbine by pili attachment and excluded volume effects While Neisseria gonorrhoeae cells typically have a diplococcus shape [27], we simplify the in silico cell shape of bacteria as two-dimensional circular disks with a radius R. Please note, however, that a diplococcus shape can be also considered [19, 23–25]. Each cell possesses exactly Np pili that are homogeneously distributed on the cell outline (see Figure 1A) We approximate pili as straight lines connecting two points: their start point (also called anchor point) The distance between these two points is called the contour length lc of the pilus The turbine is described by Nturb × 2 disks (with radius R) arranged in a double row array (see Figure 1B). Since the only way how the turbine can move is by rotation, the relative locations of the turbine disks towards each other are fixed. The orientation of the turbine is described by the angle α with respect to the y-axis and the turbine center is located at the position r(turb) (see Figure 1B) Initially, a pilus protrudes with the velocity vp in a direction perpendicular to the cell surface. When a pilus reaches a specific length, drawn from an exponential length distribution with mean length lp [17, 18] it starts to retract with a velocity vr(0) This retraction continues until the pilus has a contour length lc = 0 a new pilus protrudes from the cell from the same position A pilus binds stochastically to the substrate or the turbine disks, independently whether it is protruding or retracting. In both cases, a pilus can only bind with its tip and it can bind only to either the substrate or the turbine disks. After binding, a pilus immediately starts to retract [28] The binding is modeled as a Poisson process with the binding rate κatt(sub) to the substrate and κatt(turb) to any of the turbine disks they first need to be long enough to reach the substrate with their tips this leads to a delay in the initial binding event of a newly protruding pilus but is less relevant for following attachments of pili the binding rate might be smaller to allow a pilus to protrude to reach the substrate pili that bind to the substrate the first time do so with a rate κatt(sub,0)=vp/lp the binding is modelled as a Poisson process The rate κatt(sub,0) corresponds to the binding of a pilus that protrude with the velocity vp until it reaches a length determined by an exponential distribution with mean length lp An attached pilus will generate a pulling force that acts on the cell and Each pilus is modeled as a Hookean spring with the spring constant kp a pilus is stretched due to its retraction and hence This force is proportional to the difference between the contour length lc and the length of the pilus that it would have if it was not attached we consider the case where a pilus can only generate a pulling force and no pushing force it has been shown that the pilus force affects the retraction velocity by with the stalling force Fs [22] The force of a pilus also affects the unbinding from the substrate Pilus detachment is modelled by a Poisson process with the rate for detachment from the substrate, as motivated by [18] we introduce the detachment times τdet(sub,1) and τdet(sub,2) and the detachment forces Fdet(sub,1) and Fdet(sub,2) we describe the rate by a simpler relation with the detachment time τdet(turb) and detachment force Fdet(turb). We picked a different expression for the pili-turbine detachment (in comparison to the pili-substrate detachment) because in general, the turbine does not necessarily have to consist of the same material as the substrate, which determines pili binding properties [16, 17] we will explore turbine rotation in dependence on pili-turbine interactions thus favouring a simpler single exponential form We model pili as Hookean springs with the spring constant kp. For a cell i at location ri(cell), an attached pilus j causes a force Fi(pilusj) at the pilus anchor point ri(anchorj) on the surface of the cell in the direction of the point ri(attj) where the pilus tip is attached (see Figure 1C) In the overdamped limit [29] a force mediates a translational motion of the cell with the velocity and the torque leads to a rotation with the angular velocity vector we introduce the translational mobility μt and the rotational mobility μr The same forces and torques cause an equivalent displacement of the pili anchor points ri(anchorj) and attachment points ri(attj) in case of attachment to a turbine disk The mobilities introduced here are a result of friction with the viscous solvent and the substrate on which the cells are moving The turbine is only able to undergo rotational motion The total torque acting on the turbine is given by The rotation of the turbine is modelled in the overdamped limit with a mobility μturb 2.5 Parameters and Details of Numerical Solution We do not expect that this is a problem in our system due to the stochastic nature of the pili binding and unbinding which basically represents our system as a series of many short time events continuously interrupted by rearrangements in the pili network we do not expect any differences in the qualitative outcome of the simulations If not stated otherwise, we use the parameters provided in Table 1 Most parameters we use are based on previous studies The excluded volume spring constants k(c−c) k(turb) and k(wall) have no effect on the simulation outcome as long as they are chosen large enough to be able to compete with the pili forces we do not expect a qualitative difference in the results of the simulation We initialize the simulation by randomly distributing the cells in the box and only analyse the turbine rotation after an initialization period of 1,000 s To calculate the angular velocity of the turbine rotation with Δt = 0.5 s and the turbine orientation α (see Figure 1B) we first demonstrate that bacteria binding to the turbine with the help of pili can cause a persistent turbine rotation over time scales that exceed the pili detachment times significantly we study how the persistent turbine rotation depends on the binding properties of the pili to the turbine and the number of cells in the system This allows us to unravel the underlying mechanism that causes the persistent rotation of the turbine we propose a system where an asymmetric binding of the pili to the turbine causes a permanent unidirectional rotation of the turbine we compute the angular mean squared displacement (angular MSD) where trajectories of the angle α move persistently in one direction over periods of hundreds to thousands of seconds before the rotation turns towards the opposite direction implying that while the rotation is persistent over a certain time scale for larger time intervals it becomes random again To learn more about how persistent the turbine rotation is we compute the angular velocity autocorrelation function (angular velocity ACF) Independently of whether pili can bind to the turbine or not, we find that the correlation function ν(Δt) is decaying with time (see Figure 2D) This confirms our previous observation for the angular MSD where we found that for very large time differences the turbine rotation is diffusive and no longer shows signs of persistence We also find that for τdet(turb)=50s the normalised angular velocity ACF ν(Δt)/ν(0) has the slowest decay This behaviour correlates with the persistent rotation for time differences between Δt ≈ 10 we find that the decay of the angular velocity ACF is faster for τdet(turb)=2s than for the case where pili do not bind to the turbine We will provide an explanation for this behaviour in Section 3.3 we will have a closer look at how the turbine rotation depends on the cell adhesion to the turbine disks and the number of cells in the system We begin with a systematic analysis of how the angular MSD δα(Δt) depends on the detachment time of pili from the turbine disks τdet(turb), shown in Figure 3A For small time differences Δt < 10 s we find that δα is independent of τdet(turb) and exhibits a linear scaling For high enough values of the detachment time τdet(turb) and Δt > 10 s The duration of this superdiffusive regime increases with increasing τdet(turb) and is barely observable for detachment times τdet(turb)<10s For even longer time differences Δt Dependence of the turbine rotation on pili detachment time τdet(turb) and cell density ρ (A,C) Angular mean squared displacement (angular MSD) δα(Δt) of the turbine angle α as a function of the pili-turbine detachment time τdet(turb) (with the cell density at ρ =0.1 μm2) or cell density ρ (with the detachment time τdet(turb)=50s) (B,D) Normalised angular velocity autocorrelation function (angular velocity ACF) ν(Δt)/ν(0) of the turbine angle α as a function of the pili-turbine detachment time τdet(turb) or cell density ρ The inset figure shows the characteristic time scale of ν(Δt) resulting from a fit ν(Δt)∝exp−Δt/τ To account for the initial drop of the angular velocity ACF we only do the fitting for Δt >2s An example fit is shown in (B) (black dashed line) Please note that for the investigation of the cell density dependence of the turbine rotation we only consider a smaller range of Δt to avoid too high numerical cost we do not show the diffusive regime in the MSD for large values of Δt since the angular velocity ACF is decaying with increasing Δt Next, we investigate the normalised angular velocity ACF ν(Δt)/ν(0) (see Figure 3B) and find that for Δt < 2 s it exponentially decreases with the Δt By fitting a function of the form ν(Δt)∝exp−Δt/τ to this later regime we identify the characteristic time τ of the exponential decay of ν(Δt) and can investigate its dependence on τdet(turb) We find that it is increasing with increasing detachment times τdet(turb) This is in accordance with the increasing duration of the superdiffusive regime of the angular MSD The stronger pili of a cell bind to the turbine the more persistently the turbine rotates in one direction before it inverts its direction the characteristic decay time τ is initially increasing with cell density ρ and then decreases when ρ increases further we provide qualitative arguments that explain the observed behaviours when multiple cells bind to the same region of the turbine they will interact via excluded volume effects This will lead to additional forces acting on the involved pili and thus Overview of the mechanism driving the persistent rotation of the turbine (A) A cell (light blue circle) with n =3 or n =0 pili attached to a turbine (dark blue circles) (B) Stochastic model of n pili attached to a turbine with pili binding (with constant rate katt) and unbinding (with constant rate kdet) (C) Mean unbinding time to reach for the first time the state n =0 in the stochastic model kdet =(exp 1)/τoff (D) Sketch of how turbine rotation is affected by multiple cells binding to the turbine surface The turbine rotates in the direction in which more cells are attached to its surface (turbine is pulled by cells) (E) Sketch of how turbine rotation leads to a pickup and binding of cells on the turbine surface the distribution of cells on the turbine surface is re-arranged up to such a degree that the turbine can change its direction of motion This will then lead to a diffusive regime again This time also corresponds to the characteristic time of the angular velocity ACF decay the turbine forgets its initial direction of motion For cases where pili bind stronger to the turbine, we expect to not only have more pili to bind to the turbine, but also more cells to be bound to the turbine with its pili. To this aim, we check the number of cells and pili bound to the turbine as a function of time (see Supplementary Figure S2) We find that after an initial phase of pili attachment for larger pili-turbine detachment times τdet(turb) more cells and more pili are bound to the turbine on average This is in agreement with our previously introduced hypothesis that the turbine exhibits persistent rotational motion due to cells that are bound to the turbine surface Next, we consider the origin of the cell density dependent rotation of the turbine, see Figure 3C and Figure 3D. If the cell density is very small, often no cell will bind to the turbine most of the time and thus, the angular MSD is initially increasing with cell density. For moderate cell densities the difference between cells attaching to different sides of the turbine (see Figure 4D) will be significant and due to the random asymmetry the turbine will rotate persistently in one direction until cells randomly unbind from the turbine more and more cells are pulling in the opposite direction of the turbine rotation A possible explanation for this is that even without pili-turbine attachment cells can still rotate the turbine due to collisions and resulting excluded volume forces we reproduce a system where cells push the turbine This process possesses its own characteristic time scales that are linked to the substrate motility of the cells the persistent rotation of the turbine originates from persistent attachment of cells to the turbine over times scales much longer than the characteristic detachment times of individual pili While the resulting rotation is indeed persistent and There is no asymmetry in the initial rotational direction of the turbine and even though the turbine can move in one direction over an extended time this rotation will at some point reverse direction we will provide an example of how cells using type IV pili can drive a permanent unidirectional rotation of a turbine In order to produce a permanent unidirectional rotation of the turbine, an asymmetry of the turbine is required. Typically, such assymetries are created by altering the turbine geometry [7, 8, 12], but here we have a chance to exploit asymmetries in the adhesion of cells to the turbine instead. In Figure 5A, Figure 5B and Supplementary Movie S4 we provide an example of how altered pili binding properties on one side of the turbine wings can lead to a unidirectional rotation we consider different cases: 1) pili bind stronger to the manipulated turbine disks corresponding to a larger detachment time τdet(turb) or 2) pili bind weaker to the manipulated turbine disks Depending on how pili bind to these regions the direction of the turbine is affected as cells are more or less strongly adhered to the manipulated region and thus more or less cells are bound to these sides of the turbine and mediate the turbine rotation the turbine rotates in the direction of manipulated region because more cells are attached there for 2) less cells are attached in the manipulated region and the turbine rotates in the opposite direction Unidirectional persistent turbine rotation is caused by asymmetric pili binding to the turbine (A) Overview of the different types of turbine disks considered here (B) Angle α of the turbine as a function of time t for different combinations of the manipulated turbine disks We also provide the average angular velocity ⟨ω⟩ and its standard error for each condition (C) The angular mean squared displacement exhibits superdiffusive (ballistic) scaling (D) The normalised angular velocity auto correlation function is no longer decaying with increasing time differences Δt corresponding to the unidirectional rotation We see evidence that the rotation is permanently unidirectional in the angular MSD (see Figure 5C) which is superdiffusive (ballistic) for arbitrary time differences Δt, equivalent to a rotation in one direction. Additionally, the normalised angular velocity ACF (see Figure 5D) is no longer decaying with time Δt suggesting a constant rotation in the same direction In experiments, such manipulated regions on the surface of the turbine could be generated by coating it with a chemical such as BSA, which has been shown to alter the detachment time of the pili [17, 18, 32] we investigated how bacteria that use type IV pili for surface motility may drive the rotation of a micron-sized turbine We found that due to spatial asymmetries in the amount of cells that are bound to the turbine a persistent rotation is observed over time scales that can strongly exceed the characteristic time scales of the pili The persistent rotation is enhanced when pili bind more strongly to the turbine and weaker when the density of cells becomes larger The observed persistent rotation of a symmetric turbine has a characteristic time scale the rotation direction is reversed stochastically and there is no preferred direction of rotation A persistent and unidirectional rotation can be generated by altering the binding properties of the type IV pili on parts of the turbine in an asymmetric manner so that the only degree of freedom is the turbine rotation The impact of this aggregation process on the turbine rotation remains unclear The source code of the computational model can be accessed via https://github.com/wolframponisch/Pili-Driven-Bacterial-Turbine The Matlab scripts used to analyse the data and generate the figures are available upon request WP kindly acknowledges support from the Herchel Smith Fund (Herchel Smith Postdoctoral Fellowship) WP and VZ would like to thank the MPI-PKS IT department for access and help with the computer cluster The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fphy.2022.875687/full#supplementary-material Supplementary Figure S1 | Turbine angle α as a function of time t > 50000 s after the simulations started for three cases: Small Pili-turbine detachment rate τ (turb) det = 2 s larger τ (turb) det = 50 s and no attachment of pili to the turbine The turbine seems to be constantly rotating and does not exhibit extended periods without motion Supplemental Figure S2 | Number of cells that are attached to the turbine via its pili number of pili attached to the turbine and number of pili per cell attached to the turbine as a function of time for two different cases: pili-turbine detachment rate τ (turb) det = 2 s and τ (turb) det = 50 s We find that for τ (turb) det = 50 s the highest number of pili and cell are bound to the turbine we observe an intitial phase where the number of attached cells and pili increases before reaching a plateau This corresponds to the phase where randomly distributed cells move into the vicinity of the turbine and binding to it randomly Supplementary Movie S1 | Movie of turbine in a bath of twitching bacteria with pili-turbine detachment time τ (turb) 378 ret 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This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use *Correspondence: Wolfram Pönisch, d3AyNjlAY2FtLmFjLnVr; Vasily Zaburdaev, dmFzaWx5LnphYnVyZGFldkBmYXUuZGU= Nike’s N7 Collections feature “colors and materials of elemental forces while embodying the values and diversity of Indigenous traditions and craft,” according to Nike’s website “I’m so honored to join the Nike Family as an N7 athlete,” Pili said in a statement and I look forward to making an impact in the league and demonstrating to all the Indigenous and Polynesian girls who love the game After Friday’s Lynx game against the Phoenix Mercury Nike will hold a short ceremony in which Pili will meet with Arizona Indigenous leaders Pili’s parents will also be present and are in Phoenix to celebrate Pili’s 23rd birthday on Saturday Proceeds from the N7 Collection support “the N7 Fund to provide sport and physical activity programming to kids in Native American and Aboriginal communities,” according to Nike’s website Other athletes featured with N7 include Olympic hammer thrower Janee’ Kassanavoid whose mother was a member of the Standing Rock Sioux Tribe Pili was claimed off waivers by the Seahawks on Nov the same day the team announced it was waiving starting linebacker Tyrel Dodson Pili was previously with the Miami Dolphins who claimed Dodson — making a pseudo-trade out of the subsequent moves However, Pili was waived Saturday to make room on the 53-man roster for tight end Tyler Mabry and wide receiver Cody White. Mabry was waived from the active roster on Monday while Pili is now coming back on the practice squad It's unclear whether Mabry will also return but he's been with Seattle for nearly five seasons He was signed to the roster Saturday to account for injuries to tight ends Noah Fant and Brady Russell Jaxon Smith-Njigba Emerging as Budding Star in Seahawks' Aerial Attack Ernest Jones, Seahawks' Defense Flash 'Special' Potential in Win vs. 49ers Seahawks Waive TE Tyler Mabry Game Recap: Geno Smith's Game-Winner Snaps Seahawks' 6-Game Skid vs. 49ers Geno Smith, Seahawks Alive and Well in NFC West Race After 20-17 Win vs. 49ers CONNOR BENINTENDI With the team’s preseason opener set for Friday the defensive tackle from Anchorage is looking forward to making a strong impression on coaches and executives again heading into his second season in the NFL “Preseason last year was really good for me but it’s a new season and as they say over here ‘It doesn’t really matter what you did last year it’s what you do now.’ So I’m looking forward to and really excited for the opportunity to get to play again.” he knows he has to maintain that hungry mind-set Being a former undrafted free agent rarely ever comes with the same job security as a highly drafted player the grind to move off the roster bubble begins anew “I feel like I just kind of tweaked and adjusted to some things that I learned my rookie year but the mind-set is still the same,” Pili said “Being an underdog from Alaska where people usually don’t make it out.” Pilis said the biggest adjustment he had to make going from college to the pros and now heading into Year 2 has been paying more attention to detail and refining both his technique and overall approach to what it takes to be a professional athlete “There are a lot of things from high school ball to college ball that you have to pick up on and adjust to and it’s the same from college to the NFL,” Pili said “Everything just gets a little more technical and every little half a second counts Every step counts so you have to be more on top of your stuff.” The Dolphins have a “whole new defensive line group” following the loss of one of their most prominent leaders Now Pili is learning from seasoned veterans such as future Hall of Fame defensive end Calais Campbell who came into the league in 2008 and is entering his 17th season “He’s got a lot of ball knowledge and things we can kind of pick up from.” Miami also brought in a new defensive coordinator in Anthony Weaver where he served as a defensive coach and assistant head coach “It’s been a process just learning a new defense and everything but I think coach Weaver is a really good dude and I like his defensive philosophy and I like him as a person,” Pili said The Dolphins didn’t rotate their interior defensive linemen much last year with both Wilkins and fellow veteran stalwart Zach Seiler having played 78 percent or more of the team’s total defensive snaps, according to Pro Football Reference the Ravens rotated their defensive lineman so often that the highest percentage of total snaps for any player was second-time All Pro and Pro Bowler Justin Madubuike at 65 percent Madubuike still managed to lead all interior defensive linemen in the league with a career-high 13 sacks “It’s been really fun getting to know coach Weaver and playing in his defense,” Pili said Pili’s first year in the league was filled with fond memories that he’ll cherish forever At the top of his list was taking his first snap in a regular-season game that came in the season-opener on the road in a place he is very familiar with The Dolphins played the Los Angeles Chargers at SoFi Stadium which is less than half an hour from where he played his college home games at the University of Southern California for the Trojans ”That was pretty cool and a big moment,” Pili said What made his NFL debut even more special was the fact that several members of his family were in attendance “It was nice and it was surreal just having my mom there,” Pili said “I met up with them after the game and they were just excited and proud.” The Dolphins were the team featured on last year’s in-season version of HBO’s “Hard Knocks” which was a “pretty cool experience” for Pili in his first year in the league “Obviously being a rookie and not getting too much playing time I didn’t get a lot of camera time but it was pretty cool just to see how we go about our daily stuff as professional athletes being out there so my family got to watch and see a little bit of what I go through on a day-to-day basis,” he said While Pili wasn’t heavily featured in the docuseries he could be seen sitting front and center for every team meeting when head coach Mike McDaniel addressed everyone you gotta put your head down and learn and do what they ask you to do so I didn’t mind it,” he said “I know everybody has got to put in their time and pay their dues.” he was present when Dolphins starting quarterback Tua Tagovailoa got signed to the biggest contract in franchise history and ever given to a Polynesian player which meant a lot to him as a fellow member of the Pacific Islander community “His family was over here at the facilities when it happened and I’ve been to his house a few times and hung out with him a little bit Pili is not the only member of his family that has gone pro in the last year. His younger sister Alissa was selected No. 8 overall in the 2024 WNBA Draft by the Minnesota Lynx in April While he wasn’t able to share that moment with her in person due to his professional obligations he was extremely elated for her all the same and I was really excited for her,” Pili said The type of media coverage she is getting now [From Lynx to Lynx: Alissa Pili’s basketball journey comes full circle in a place that ‘was meant to be’] He has been able to catch a few of her games in person since she was drafted She only saw the court for a handful of minutes but Brandon can relate to sitting behind a group of established veterans there were a lot of good veterans ahead of me and it’s just good to learn from them and take it all in,” Pili said His advice to her heading into her rookie season was “there’s going to be some ups and downs but just stay the course and you’ll be alright.” Pili was able to spend some time back home in Alaska and see family this summer during the offseason “It’s important to have balance in everything you do,” he said “Being away from them for so long and putting in the hours of work it’s good to go see family and take time off He is the oldest of eight siblings and it was “super important” to get to see them A contingent might be coming down to the Sunshine State to watch him get extended playing time in one of the Dolphins two home preseason games Miami will open the preseason Friday at Hard Rock Stadium against the Atlanta Falcons at 3 p.m Pili isn’t the only player who was hails from the Last Frontier in the NFL Baltimore Ravens offensive lineman Malaesala Aumavae-Laulu of Palmer Buffalo Bills linebacker Edefuan Ulofoshio of Anchorage and Las Vegas Raiders defensive end Tyree Wilson of Anchorage are also in training camps “I think it’s really cool because you don’t see (it often) and a lot of people that I’ve met don’t expect there to be football in Alaska,” Pili said “Just seeing other people from there being a role model to the kids in Alaska and giving them hope to one day try anything.” Pili’s advice to the next generation of young Alaskans who want to follow in the footsteps of those players and make it to the highest level of any professional sport is to have the utmost belief in themselves. “Belief in yourself is big,” he said. “Just believe in yourself. You’ve got to work real hard and just try to create relationships that will help you get into the right field of what you’re trying to pursue.” Pili was lost for the season to a torn ACL in the 23-17 overtime win against Florida Saturday night BEAT BAMA pic.twitter.com/e3caY9Qtu6 Pili was on the field for just two snaps before appearing to suffer a leg injury He walked off under his own power after being examined by Tennessee trainers then was quickly ruled out for the rest of the game Pili is tied with Will Brooks as Tennessee’s leading tackler through the first six games of the season, with 29.0 total tackles (11 solo, 18 assists), including 1.5 tackles for loss. He also has two quarterback hits. He had a season-high 10 tackleT in Tennessee’s 19-14 loss at Arkansas. Pili had five tackles at Oklahoma, three tackles against Kent State, four against North Carolina State and two in the opener against Chattanooga. He had 0.5 tackles for loss against NC State and 1.0 at Oklahoma. Pili transferred to Tennessee from BYU before last season but he was injured in the season-opening win over Virginia at Nissan Stadium in Nashville and was lost for the season “I’m not sure there’s a better representative of what a Volunteer is than Keenan Pili,” Tennessee coach Josh Heupel said Saturday night. “You talk about a selfless guy that cares about the people around him way more than he cares about himself.  “That’s an elite competitor, great leader, tough as nails, athletic, explosive, everything that you want. Just he’s a dude, man. Special dude.” USA Today on Monday released its ranking of the top 25 coaches in all of College Football, with Tennessee's Josh Heupel making the cut.... Tennessee's Jermod McCoy is widely projected to be a first-round pick in the Way-Too-Early 2026 NFL mock drafts.... Former Tennessee offensive lineman Ayden Bussell announced his new commitment out of the NCAA Transfer Portal on Sunday.... Tennessee AD Danny White continues to try and prepare for the next generation of college athletics amid more unknowns than knowns.... Tennessee continues to work the transfer portal in football. Hoops is elite with what they have assembled and football recruiting heating up.... Former Tennessee Football safety Jakobe Thomas committed to Miami on Thursday night. Thomas reportedly visited only the Hurricanes and Florida State... Tennessee head coach Josh Heupel said it's a ground zero start to the summer at the quarterback position as well as other youthful spots.... Tennessee head coach Josh Heupel had a new name for the Nico Iamaleava situation on Wednesday: The Joey Aguilar situation.... Join the Volquest crew of Brent Hubbs, Austin Price and Grant Ramey as they discuss these Tennessee topics and plenty more on the weekly mailbag... What Tennessee Football coach Josh Heupel said while meeting with reporters Wednesday night at the Big Orange Caravan in Nashville.... Tennessee head coach Josh Heupel doesn't have a GM by title in his program. Heupel and Tennessee AD Danny White discussed the position.... Tennessee wide receiver Chris Brazzell acknowledges last year wasn't good enough. To improve, Brazzell focus is on flushing things better.... Two former Tennessee Football players committed to the Colorado Buffaloes on Wednesday out of the NCAA Transfer Portal.... In what sure looks like a trade, Tennessee has added UCLA quarterback Joey Aguilar after former Vol quarterback Nico Iamaleava picked UCLA on earlier... Tennessee has sold out its season-ticket allotment for a third straight year, while nearly 24,000 fans are on a waiting list for tickets.... Tennessee continues to work the portal. The Vols are still looking for a defensive lineman, a receiver and they are set to host two DB's.... Tennessee continues to look for help at defensive tackle as well as wide receiver in the portal. Hoops is also looking for roster additions... Tennessee Football transfer defensive back Jakobe Thomas has set two visits, according to a report from On3's Pete Nakos.... Tennessee redshirt freshman quarterback Jake Merklinger has agreed to a new contract, according to On3's Pete Nakos.... Boo Carter believes Nico Iamaleava should have handled his exit at Tennessee better, calling the exit "shocking."... The 2025 NFL Draft is officially in the books, and four Tennessee Volunteers heard their name called throughout the weekend, including James Pearce... The 2025 NFL Draft is underway, and record-setting Tennessee Volunteers Running Back Dylan Sampson is officially off the board in the 4th round after... The 2025 NFL Draft continues on, and another Tennessee Volunteer is officially off the board in the X round after wide receiver Dont'e Thornton was... Where Dylan Sampson and other Tennessee prospects are ranked ahead of Day 3 of the NFL Draft Saturday in Green Bay.... Omarr Norman-Lott became the second Tennessee Volunteer to be selected in this year's 2025 NFL Draft after being selected in the second round by the... Waived and then placed on the practice squad in November Pili was signed to a reserve/future contract in January 2024 He looks to be on a long list of training camp contenders within the defensive line for depth chart positioning Pili played a month’s worth of games as a rookie before a few weeks of inactivity then ultimately a demotion to the practice squad During the Dolphin’s opening four games last season Pili played a reserve role on the defensive line’s interior seeing just 30 snaps and 21 on special teams he was able to see a bit more time in Miami’s 70-20 blowout win over the Denver Broncos Pili played in 23% of the team’s defensive snaps recording only two tackles on the season during the Week 3 home opener The Dolphins and Pili agreed to a two-year including an average annual salary of $1.04 million In 2024, Pili will earn a base salary of $915,000, while carrying a cap hit of $915,000 (per spotrac.com) Pili has plenty of competition this summer Da’Shawn Hand returns to Miami and the Dolphins will also have 2024 undrafted free agents Mario Kendricks and Leonard Payne battling Pili and company is the elder statement with Christian Wilkins now in Las Vegas with the Raiders Miami also added 16-year veteran Calais Campbell to the line New defensive coordinator Anthony Weaver’s system deploys a rotation of interior linemen so Pili could have a fighting chance to not only make the team but stay on it through the season He will certainly have his work cut out for him and it will be interesting to see him compete with free agents Tart and Jones especially -- Dolphins Training Camp Preview: LB Jaelan Phillips -- Dolphins Training Camp Preview: CB Nik Needham -- Dolphins Training Camp Preview: CB Siran Neal -- Dolphins Training Camp Preview: RB Raheem Mostert -- Dolphins Training Camp Preview: RB De'Von Achane -- Dolphins Training Camp Preview: RB Salvon Ahmed -- Dolphins Training Camp Preview: T Terron Armstead -- Dolphins Training Camp Preview: LB Shaquil Barrett -- Dolphins Training Camp Preview: WR Odell Beckham Jr. -- Dolphins Training Camp Preview: WR Braxton Berrios -- Dolphins Training Camp Preview: CB Ethan Bonner -- Dolphins Training Camp Preview: OL Aaron Brewer -- Dolphins Training Camp Preview: RB Chris Brooks -- Dolphins Training Camp Preview: LB Jordyn Brooks -- Dolphins Training Camp Preview: DB Elijah Campbell -- Dolphins Training Camp Preview: OLB Bradley Chubb -- Dolphins Training Camp Preview: TE Tanner Conner -- Dolphins Training Camp Preview: OL Lester Cotton -- Dolphins Training Camp Preview: WR River Cracraft -- Dolphins Training Camp Preview: OL Jack Driscoll -- Dolphins Training Camp Preview: OL Liam Eichenberg -- Dolphins Training Camp Preview: WR Erik Ezukanma -- Dolphins Training Camp Preview: TE Jody Fortson -- Dolphins Training Camp Preview: CB Kendall Fuller -- Dolphins Training Camp Preview: DT Neville Gallimore -- Dolphins Training Camp Preview: DT Da'Shawn Hand -- Dolphins Training Camp Preview: DT Jonathan Harris -- Dolphins Training Camp Preview: TE Julian Hill -- Dolphins Training Camp Preview: S Jevon Holland -- Dolphins Training Camp Preview: FB Alec Ingold -- Dolphins Training Camp Preview: T Austin Jackson -- Dolphins Training Camp Preview: DT Benito Jones -- Dolphins Training Camp Preview: OL Robert Jones -- Dolphins Training Camp Preview: CB Kader Kohou -- Dolphins Training Camp Preview: T Kendall Lamm -- Dolphins Training Camp Preview: LB David Long Jr. JASON SARNEY she’s found joy as a rookie on a Lynx team that finds itself one game away from a WNBA championship MINNEAPOLIS — Alissa Pili is used to being a star — the Minnesota Lynx rookie has effectively dominated every level of basketball since she was a kid As a freshman at the University of Southern California and in her final season at her University of Utah she was one of the most efficient high-volume scorers in the country averaging 21.4 points per game on 55% shooting her on-court opportunities have been limited; she appeared in 22 games during the 2024 season averaging 2.4 points in 6.3 minutes per outing Going from college star to predominantly riding the bench hasn’t come without its challenges “It’s definitely taken a toll on me mentally,” Pili told SB Nation thanks to the unique connection and camaraderie in the Lynx locker room and unwavering support from Cheryl Reeve and the coaching staff “To find ways to help the team and impact the team — not only on the court — was the biggest thing for me to learn,” Pili said still being in good communication with my coach and the team and talking to them about where my head’s at and any struggle that I go through.” Now, Pili is headed to Game 5 of the WNBA Finals and while she’s only checked in once in this postseason the 23-year-old is thoroughly enjoying the journey of being apart of on one of the best teams in the world and learning from her more veteran teammates “There’s not a lot of rookies that get to be in this position I’m in and the fact that our team is so successful has been really fun to be a part of,” Pili said “It’s just been a very great learning experience Alissa Pili has shared those sentiments since she arrived to training camp just a few weeks after the conclusion of her college season “Something that kind of caught me off guard was honestly just how well this team clicked together so fast,” Pili said “I’ve seen it from college — any team who goes and just grabs a whole bunch of new players it’s super hard to build that chemistry and be good right away said she prioritizes connection and community the fact that she felt so embraced by the locker room and hit it off with her teammates right away so that’s a value that I bring into what I’m doing,” Pili said and that’s a quality that I bring to my team Alissa Pili vs Myisha Hines-Allen arm wrestling pic.twitter.com/R12jQ7lX1F rather than sulk at the limited on-court opportunities she has embraced finding her voice in the locker room and being a positive spirit on the bench “The biggest thing was me finding my footing with where I stand on the team and what I can do to contribute if I’m not going to be out there on the floor all the time,” Pili said “Nobody wants somebody who’s just taking energy from the team and being pissed off that they’re on the bench.” There was one on-court this season that gave Pili a peek into what her future in the WNBA might look like a May 31st game against the Phoenix Mercury in which she exploded for 20 points on 7-9 shooting in just 15 minutes of action she made all 4 of her three-point attempts showcasing a glimpse of how her game could translate at the professional level ALISSA PILI — BREAKOUT 20 POINT GAME ‼️ pic.twitter.com/uAvoWqDsbT “It definitely just gives me confidence,” Pili said “I may not be ready to compete consistently at a high level in the league yet and have had moments where I have done well has given me hope and confidence moving forward.” staying positive about limited playing time hasn’t been too difficult simply because the experience of being a Lynx has been so enjoyable I wasn’t playing a minute in the whole season when I was a rookie,’” Pili said “They just let me know my time is coming.” The WNBA rookie has shown flashes as she adjusts to the professional game as a member of the Minnesota Lynx PHOENIX — Laney Maria Lupe remembers when Alissa Pili was still playing for the University of Utah Having attended a game against the University of Arizona in Tucson she recalled her initial conversation with the Minnesota Lynx rookie forward and the devout connection they shared Friday night in downtown Phoenix was no different and her Lynx team were in the city to play the Phoenix Mercury “I gifted her an eagle feather because it has significance with Indigenous people,” Lupe who currently holds the title of Miss Indian Arizona for the 2023-24 year wisdom and guidance throughout her journey.” has followed Pili throughout her career and understands the significant role she plays as an up-and-coming Indigenous basketball player dressed in her Miss Indian Arizona regalia chatted some with Pili before the game when the rookie walked around near her bench and signed autographs for fans and posed for photos “That's my main reason for coming here,” Lupe said “(I’m) letting her know that we're all behind her because she's the one that's breaking down barriers and walls for us.” Pili checked into the game for the first time late in the third quarter to a roar of the crowd ending with a game-winning shot by Phoenix with less than a second to play chats with Miss Indian Arizona Laney Maria Lupe and other fans before the game against the Phoenix Mercury in Arizona before the start of the Phoenix Mercury game in Arizona short chants by some fans asking for Pili could be heard sporadically in the arena Pili took one shot after checking but went scoreless in her four minutes played Pili averages around six minutes a game and has played in 9 of the team's first 12 games she exploded for 20 points in a win against the Mercury in Minnesota Pili signed an endorsement deal with Nike as a N7 athlete The announcement was made on Friday and Nike held a private ceremony after the game to recognize her I wanted to include my heritage,” Pili told ICT before Friday’s game “I wanted to include the people who have supported me along the way and represent them and myself.” The deal aims to provide Indigenous communities with inclusivity and equity both on and off the court an opportunity that Pili believes will benefit aspiring youth She joins a growing list of Indigenous athletes playing a variety of professional sports “I hope that me being in this position has a lot of impact and opens that pathway for (them),” Pili said “I’m always somebody who’s going to support my people …I carry that with pride and I hope that people don’t settle no matter what culture they come from.” Before she was selected in the first round of the 2024 WNBA draft Pili was a three-time Alaska Gatorade Player of the Year winner and Pac-12 Player of the Year Laura Tina Poyer and her young daughter Malia were in the stands near the Lynx bench one had a photo of Malia with Pili when she played for Utah The other sign had a Samoan greeting along with Pili's name we are Samoan and it's awesome to see someone from our culture our roots in the WNBA,” Laura Tina Poyer said "We follow her current team right now I think she's very versatile on the floor She's a beast in the paint," Poyer added Fans of Minnesota Lynx rookie Alissa Pili Although she’s still adjusting to the WNBA’s intense pace and starting with a new team Pili said the support she’s received has only grown since playing professionally “In college I had a bunch of support and it’s only carrying over and getting bigger,” Pili said “This league does a great job of just uplifting women Everybody is very supportive of each other so it’s a super cool thing to be a part of.” ICT’s Dalton Walker contributed to this report Leah Mesquita is a student at Arizona State University and ICT intern for the 2024 summer.  Alissa Pili #35 of the Minnesota Lynx is taking to the court a WNBA basketball game between Minnesota Lynx and Seattle Storm on May 17 in Minneapolis.Kerem Yücel | MPR NewsGo Deeper.CloseCreate an account or log in to save stories We have added it to a list of your favorite stories This story comes to you from ICT through a partnership with MPR News. Laney Maria Lupe remembers when Alissa Pili was still playing for the University of Utah. MPR News helps you turn down the noise and build shared understanding. Turn up your support for this public resource and keep trusted journalism accessible to all. Having attended a game against the University of Arizona in Tucson, she recalled her initial conversation with the Minnesota Lynx rookie forward and the devout connection they shared, even before Pili’s WNBA debut. Friday night in downtown Phoenix was no different. Pili, Samoan and Iñupiaq, and her Lynx team were in the city to play the Phoenix Mercury. “I gifted her an eagle feather because it has significance with Indigenous people,” Lupe, who currently holds the title of Miss Indian Arizona for the 2023-24 year, said. “(It’s for) strength, wisdom and guidance throughout her journey.” Lupe, like many other Indigenous fans, has followed Pili throughout her career and understands the significant role she plays as an up-and-coming Indigenous basketball player. Lupe, dressed in her Miss Indian Arizona regalia, chatted some with Pili before the game when the rookie walked around near her bench and signed autographs for fans and posed for photos. “That’s my main reason for coming here,” Lupe said. “(I’m) letting her know that we’re all behind her because she’s the one that’s breaking down barriers and walls for us.” Alissa Pili (35) of the Minnesota Lynx in action during the WNBA basketball game between the Minnesota Lynx and the Seattle Storm on May 17 in Minneapolis.Kerem Yücel | MPR NewsPili checked into the game for the first time late in the third quarter to a roar of the crowd. The game was a thriller, ending with a game-winning shot by Phoenix with less than a second to play. The Mercury won, 81-80. Before Pili checked in, short chants by some fans asking for Pili could be heard sporadically in the arena. Pili took one shot after checking but went scoreless in her four minutes played. So far in her young WNBA career, she has come off the bench. Pili averages around six minutes a game and has played in 9 of the team’s first 12 games. Just a week before coming to Arizona, she exploded for 20 points in a win against the Mercury in Minnesota. Despite her rookie status, Pili signed an endorsement deal with Nike as a N7 athlete. The announcement was made on Friday and Nike held a private ceremony after the game to recognize her. “When I talked to my agent about marketing myself in that area, I wanted to include my heritage,” Pili told ICT before Friday’s game. “I wanted to include the people who have supported me along the way and represent them and myself.” Lupe Fruean 16, (right) and Lelia Fruean,14 (left) chanting for Alissa Pili #35 of the Minnesota Lynx during the WNBA basketball game between the Minnesota Lynx and the Seattle Storm on May 17 in Minneapolis.Kerem Yücel | MPR NewsThe deal aims to provide Indigenous communities with inclusivity and equity both on and off the court, an opportunity that Pili believes will benefit aspiring youth. She joins a growing list of Indigenous athletes playing a variety of professional sports. “I hope that me being in this position has a lot of impact and opens that pathway for (them),” Pili said. “I’m always somebody who’s going to support my people. …I carry that with pride and I hope that people don’t settle, no matter what culture they come from.” Before she was selected in the first round of the 2024 WNBA draft, Pili was a three-time Alaska Gatorade Player of the Year winner and Pac-12 Player of the Year. Laura Tina Poyer and her young daughter Malia were in the stands near the Lynx bench. Both had signs, one had a photo of Malia with Pili when she played for Utah. The other sign had a Samoan greeting along with Pili’s name. Fans are chanting for Alissa Pili #35 of the Minnesota Lynx during the WNBA basketball game between the Minnesota Lynx and the Seattle Storm on May 17 in Minneapolis.Kerem Yücel | MPR News“She’s Samoan Native, we are Samoan and it’s awesome to see someone from our culture, our roots in the WNBA,” Laura Tina Poyer said. “We follow her current team right now, I think she’s very versatile on the floor. She’s a beast in the paint,” Poyer added. Although she’s still adjusting to the WNBA’s intense pace and starting with a new team, Pili said the support she’s received has only grown since playing professionally. “In college I had a bunch of support and it’s only carrying over and getting bigger,” Pili said. “This league does a great job of just uplifting women, not only the league, but players as well. Everybody is very supportive of each other, so it’s a super cool thing to be a part of.” ICT’s Dalton Walker contributed to this report. was recently drafted by the Minnesota Lynx in this year's WNBA draft Known for her tenacity and skill on the court Pili brings a unique heritage and perspective to the league Her signing with Nike N7 highlights her exceptional talent and dedication to representation and advocacy for Indigenous and Polynesian communities Pili expressed her enthusiasm and sense of duty "I’m so honored to join the Nike Family as a Nike N7 athlete that they can reach their dreams." These words resonate with her journey and the broader mission of Nike N7 Pili's partnership with Nike N7 is expected to inspire many young athletes who see themselves reflected in her story Her role as a Nike N7 athlete will undoubtedly amplify Indigenous and Polynesian communities' voices spotlighting their rich cultures and the importance of inclusivity in sports Enjoy all the latest sneaker raffle and releases and exclusive sneaker news in the palm of your hand Alaska native who of Iñupiat and Samoan heritage was selected by the Lynx as the eighth overall selection in the 2024 WNBA Draft She has averaged 3.6 points and 1.3 rebounds in seven games off the bench for the 7-2 Lynx this season The company's website says that since launching the N7 brand in 2009 it has donated over $10.8 million to nearly 300 organizations supporting Indigenous communities "I’m so honored to join the Nike Family as a Nike N7 athlete that they can reach their dreams," said Pili in a statement on social media Pili will also reportedly meet with Arizona Indigenous leaders following Minnesota's game Friday night in Phoenix Tipoff for that game between the Lynx and Mercury is set for 9 p.m Apr 15, 2024; Brooklyn, NY, USA; Alissa Pili poses with WNBA commissioner Cathy Engelbert after she is selected with the number eighth overall pick to the Minnesota Lynx in the 2024 WNBA Draft at Brooklyn Academy of Music. a screening of the movie Sight and Sound says is the best movie ever made saying he'd killed the mother of his child 60 IN 60 1:41 PM | Updated: Jun 7 Utah State picked up a commitment from former BYU linebacker Logan Pili BY KSL SPORTS SALT LAKE CITY – The countdown for Hans & Scotty’s 60 in 60 is on Coming in at #59 is Utah State‘s Logan Pili (LB) A post shared by KSL Sports (@kslsports) Pili was a three-star prospect and the 11th-ranked player in the state of Utah In his senior year at Timpview High School and one interception which earned him a spot on the 2020 Polynesian Bowl roster Pili started his collegiate career by redshirting at BYU He played in he appeared in three games and had seven tackles pic.twitter.com/ZBFzLQDpTM — Logan Pili (@LoganPili8) January 25, 2024 Pili moved north in 2023 to suit up for the Utah State Aggies He played in 12 games and finished with 31 tackles who both played football with the BYU Cougars Every summer Hans and Scotty reveal the best college football players in the state of Utah in their annual 60 in 60 as voted on by the media the 60 in 60 included one vote based on a fan ballot Are you on Threads yet? Let’s connect, give us a follow @kslsports Click here for the complete 60 in 60 rankings countdown. Download the new & improved KSL Sports app from Utah’s sports leader. You can stream live radio, video and stay up to date on all of your favorite teams. Follow @kslsports Everyone on Rocky Top expects great things out of first-year starting quarterback Nico Iamaleava during his career at Tennessee According to Vols linebacker Keenan Pili who lines up against Iamaleava every day at practice he’s seen first-hand how talented the former five-star recruit was in high school Pili believes he’s proven to be a bit of a locker room leader heading into his second year with the program He’s very talented,” Pili said at SEC Media Days “The physical part is pretty easy to see when you see Nico play One thing I’ve seen from Nico over this offseason is really his mental side of the game and his growth as a player “He’s really found a way to get some respect in the locker room just his humility has drawn a lot of people to want him to lead and I think that’s kind of been one of his greatest attributes so far and the way he leads us is through that.” While the former blue-chip prospect didn’t see the field much during his first season in Knoxville, he made the most of his limited opportunities backing up former QB Joe Milton. Iamaleava went on to receive the first start of his career against Iowa in the Citrus Bowl after Milton opted out of the game ultimately defeating the Hawkeyes 35-0 having scored three rushing touchdowns on 15 attempts while going 12-of-19 passing to go with one touchdown pass and no interceptions He finished his true freshman season with 31 completions for 314 yards and two touchdowns leading many to become optimistic about his future and the success that may come with it Fans were given a preview of Iamaleava’s passing skills during April’s spring game, completing his first seven of his passes for 93 yards and a touchdown during his time on the field. He capped the first half of action for his squad with a 27-yard strike to Chas Nimrod in the end zone Iamaleava didn’t play much after the first half and finished the gae 7 of 9 for 96 yards and the lone touchdown to Nimrod There’s a lot to be excited about offensively for Josh Heupel’s group heading into 2024. Behind Pili and Iamaleava’s kead, Tennessee kick things off against in-state foe Chattanooga before travelling to Charlotte to play NC State inside Bank of America stadium in Wek 2.