OneFootball's home page Search Settings Sign In Sign InJoinSedang Berlangsung Duel Seru PSS Sleman Vs PSM Makassar pada Ajang BRI Liga 1 Ini Link Live Streaming-nya di Vidio | OneFootballBola.com Jakarta - PSS Sleman bakal menjalani laga krusial dalam lanjutan BRI Liga 1 2024/2025 Tim besutan Pieter Huistra itu meladeni perlawanan PSM Makassar pada pekan ke-31 duel antara PSS versus PSM digelar di Stadion Maguwoharjo Pertandingan kedua tim dimulai pada pukul 19.00 WIB dan disiarkan langsung Indosiar sertaa live streaming di Vidio Tidak ada pilihan lain bagi tim berjulukan Super Elang Jawa itu selain memenangkan pertandingan nanti Tambahan poin sangat penting untuk menjaga peluang bertahan di BRI Liga 1 musim depan Adapun PSM juga bertekad meraih poin maksimal demi menjaga kans finis di posisi lima besar klasemen akhir BRI Liga 1 bisa dipastikan bentrok kedua kubu akan berlangsung ketat sejak menit awal Related NewsVIDEO IN – Dopo Marotta Laporta al pranzo prima di Inter-Barcellona Domani -2 a #MilanBFC, allenamento alle 11 Conference League, Fiorentina-Betis: dove vedere il match in TV e streaming Sala sul nuovo Stadio: «Abbiamo delle questioni da sistemare. 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An official website of the United States government A .gov website belongs to an official government organization in the United States ) or https:// means you’ve safely connected to the .gov website Share sensitive information only on official Emergency Information for American Citizens in collaboration with Yayasan Pendidikan Haji Kalla on February 6 celebrated the opening of American Corner Makassar at the Bikin Bikin Creative Hub in Nipah Mall in Makassar the first American Corner in a public mall in Eastern Indonesia is designed to foster dialogue between Indonesians and Americans in support of our bilateral relationship Distinguished guests attending the opening ceremony included U.S Consul General in Surabaya Christopher Green Consulate General Surabaya Public Affairs Officer Genevieve Judson-Jourdain and Regional Public Engagement Specialist Marcy Carrel Representing the Kalla Group were former Vice President of the Republic of Indonesia and current Chairman of the Indonesia Red Cross Society HE Dr “American Corner Makassar is a space dedicated to education and partnership,” said Consul General Green “Welcoming all members of the public and offering critical resources and educational and skills building classes this space will support the human capital development essential to U.S.-Indonesian mutual prosperity in South Sulawesi and beyond Consulate Surabaya and the Kalla Foundation will expand the already strong bonds of friendship between the United States and Indonesia.” we can understand each other’s perspectives in order to build better relations,” said Jusuf Kalla  “I am sure that the American Corner will be a beacon that strengthens relations between Indonesia and the United States.” American Corner Makassar will be open seven days a week from 10:00 to 22:00 and offer a variety of programs and events that are free-of-charge and open to the public From advanced technology classes to film screenings offerings are intended to appeal to a wide range of interests American Spaces are key touchpoints for millions of people globally building connections between the United States and communities worldwide often in partnership with local institutions American Spaces are strategically aligned to U.S foreign policy and national security goals They showcase the United States’ core values and reflect the American spirit to international audiences Through their engagement with foreign audiences American Spaces support mutual prosperity and strength foster a greater understanding of the United States and offer opportunities to connect with aspiring leaders The American Spaces Indonesia network includes three American Centers:  MyAmerica Jakarta  in the U.S Embassy Jakarta; 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"root": "https:\/\/id.usembassy.gov\/wp-json\/" var wpcf7cf_global_settings = {"ajaxurl":"https:\/\/id.usembassy.gov\/wp-admin\/admin-ajax.php"}; var wpApiSettings = {"root":"https:\/\/id.usembassy.gov\/wp-json\/","nonce":"7f026e1f88","versionString":"wp\/v2\/"}; var _wpUtilSettings = {"ajax":{"url":"\/wp-admin\/admin-ajax.php"}}; var tve_dash_front = {"ajaxurl":"https:\/\/id.usembassy.gov\/wp-admin\/admin-ajax.php","force_ajax_send":"","is_crawler":"","recaptcha":[],"turnstile":[],"post_id":"21766"}; var ba_map_ajax = {"url":"https:\/\/id.usembassy.gov\/wp-admin\/admin-ajax.php","gif":"https:\/\/id.usembassy.gov\/wp-content\/plugins\/osmapper\/assets\/images\/loader.gif","defaultConfig":{"pin":"https:\/\/id.usembassy.gov\/wp-content\/plugins\/osmapper\/assets\/images\/pins\/pin-1.png","scheme":"http:\/\/basemaps.cartocdn.com\/light_all"}}; document.addEventListener("cmplz_run_after_all_scripts" function cmplz_thrive_fire_domContentLoadedEvent() { var tcb_current_post_lists=JSON.parse('[]'); var tcb_post_lists=tcb_post_lists?[...tcb_post_lists,...tcb_current_post_lists]:tcb_current_post_lists; (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start': new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0] j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src= 'https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f); })(window,document,'script','dataLayer','GTM-T8NZJH'); window.addRevokeListener = (callback) => { * Called from GTM template to set callback to be executed when user consent is provided window.addConsentUpdateListener = (callback) => { document.addEventListener("cmplz_fire_categories" var consentedCategories = e.detail.categories; 'personalization_storage': cmplz_in_array( 'preferences' 'analytics_storage': cmplz_in_array( 'statistics' 'ad_user_data': cmplz_in_array( 'marketing' 'ad_personalization': cmplz_in_array( 'marketing' as the TM template needs to be sure it's cmplz_ Irsyan Hasyim TEMPO.CO, Jakarta - Torrential downpours caused floods to inundate parts of South Sulawesi’s Makassar on Monday affecting 580 homes and displacing as many as 580 households heavy rain and the overflowing of the Walennae River have caused flooding and breached embankments at two points in Ujung Lero Village and one person is reported missing and presumed to have been swept away by the river current the Wajo Regency BPBD has carried out emergency response and search efforts for the missing victim "The floods have yet to recede and remain submerging houses and agricultural land in Wajo,” he said Extreme weather is also reported in Minahasa Torrential downpours and strong winds have toppled trees and damaged the roofs of houses in four villages in Kombi District The four affected villages are Kalawiran Village and Kolongan Village in the Kombi District with reported material losses including nine lightly damaged houses "The Minahasa BPBD has made efforts to assess the disaster site as well as distribute aid to affected residents," said Muhari where heavy rain and strong winds damaged a house in Karangasem Regency on Wednesday afternoon Three road accesses were obstructed due to fallen trees in Bebandem district and a person was reported killed in the incident BNPB urges local governments and residents to increase preparedness and vigilance in facing the disaster risks by regularly cleaning rivers and waterways Editor’s Choice: Extreme Rainfall Triggers Floods in Jakarta During Chinese New Year, Data Shows Click here to get the latest news updates from Tempo on Google News Lenovo Set to Launch ThinkPad Aura Edition Series for Professionals in Indonesia Wikipedia: AI Will Not Replace Human Volunteers Retina Scanning for Crypto? Cyber Law Expert Warns of Worldcoin's Biometric Dangers Samsung to Open Galaxy AI-Driven Pop-Up Restaurant in Portugal BMKG Reports M5.4 Earthquake Striking Tomini Bay; No Tsunami Threats Indonesia's Mount Semeru Erupts 3 Times This Morning, Sends Ash 700 Meters High WHO: Hand Hygiene is Essential, Medical Gloves Are Not a Substitute Jobs That AI Will Replace: Is Your Current Job at Risk? Top 10 Highest-Paying Majors Worth Pursuing in 2025 Expert Raises Alarm on Crypto Scams, Biometric Leaks After Worldcoin Freeze Coastal Flood Submerges Neighborhood Unit in North Jakarta: BPBD BMKG Warns of Extreme Weather Across Indonesia Yogyakarta Flood Causes Underground River at Pantai Baron to Overflow and Turn Brown 27 Houses Destroyed as Massive Fire Erupts in Indonesian Village Near Lake Toba Govt Warns Travelers of Extreme Weather Before Eid al-Fitr Heavy Rainfall Forecasted Across Saudi Arabia Until Eid al-Fitr 2025 Heavy Rainfall Triggers Widespread Flooding in Manado City Environment Ministry Orders Sentul City to Stop Flood-Inflicted Development Prabowo Subianto Plans to Establish 100 People's Schools Soon List of Baeksang Arts Awards 2025 Winners Prabowo Praises Jokowi's Inflation Control in Cabinet Session, Denies Link to Gibran's Presence Jokowi Responds to Calls for VP Gibran's Impeachment Prabowo Claims 99.99% Success Rate for Free Nutritious Meal Program Prabowo Rejects 'Puppet President' Label, Denies Jokowi's Control Hollywood Shaken as Trump Orders 100% Tariff on All Foreign-Made Films Hasan Nasbi Cancels Resignation as Indonesia's PCO Head: 'I Am Loyal to the President' What Are the Cheapest Businesses to Start from Home? Here Are the Top 10 Hun Sen Slams Developed Nations' Meddling in Developing Countries Ready for a Change? Discover the 10 Best Countries for Job Opportunities Abroad All 133 Cardinal Electors Arrive in Rome Ahead of Papal Conclave Halal Snack Polemic: Tests by MUI Contradict BPJPH, BPOM Findings on Pork Content Deadly Bus Accident Leaves 12 Dead in Indonesia, Police: Brake Failure Bappenas, FAO Host Training Workshop to Enhance Governance in Indonesia's Agrifood Systems Transformation World Leaders Condemn Israel's Plan to 'Conquer' Gaza Trump Ends Exemption for Low-Value Chinese Imports President Joko "Jokowi" Widodo inaugurated two new state-of-the-art hospitals in Makassar marking a significant boost to Indonesia's healthcare infrastructure built at a cost of Rp 1.56 trillion ($104 million) located in the Center Point of Indonesia area is designed to serve patients from eastern Indonesia and offers advanced services for cancer “This is the largest of the eight hospitals I’ve inaugurated so far and it surpasses even five-star hotels,” Jokowi said The hospital boasts medical technology comparable to top facilities abroad with an additional Rp 500 billion allocated for advanced healthcare equipment Acting Director of the Health Ministry Hospital $104 Million Gov't Hospital to Stop People From Getting Treatment Abroad The president said the hospitals will help prevent an annual foreign exchange loss of Rp 180 trillion which is currently spent by Indonesians seeking medical treatment in countries like Singapore “We don’t want sick people to go to Singapore and Malaysia for treatment and it feels like walking into a five-star hotel," he said Sign InJoinPieter Huistra Sebut Duel PSS Sleman Vs PSM Makassar Jadi Laga Terpenting di BRI Liga 1 Musim Ini | OneFootballBola.com sadar betul betapa pentingnya laga melawan PSM Makassar pada pekan ke-31 BRI Liga 1 2024/2025 Duel kedua tim dimainkan di Stadion Maguwoharjo Tim berjulukan Super Elang Jawa itu kini berada di dasar klasemen sementara BRI Liga 1 alias peringkat ke-18 dengan perolehan 22 poin Mereka butuh tambahan angka untuk menjaga asa bertahan di Liga 1 musim depan timnya akan berusaha maksimal untuk mendapatkan poin penuh di rumah sendiri laga melawan PSM merupakan pertandingan paling penting musim ini kami sudah menjalani latihan beberapa hari kemarin dan tim mempersiapkan dengan baik untuk lawan PSM," ujarnya saat sesi konferensi pers jelang laga "Kami terus mencoba untuk mendapatkan hasil maksimal besok adalah pertandingan paling penting dan kami harus siap," sambung juru taktik asal Belanda tersebut Related NewsBRI Liga 1: Jelang Bali United Hadapi Persija Jakarta, Teco Banyak Dapat DM dari Jakmania Rencana Timnas Indonesia Vs Malaysia, Erick Thohir: Kami Sudah Kangen Lawan Harimau Malaya, Kalau Mereka Mau Persib Akan Konvoi dari Gedung Sate ke GBLA, Wagub Jabar: Kita Rayakan Sewajarnya Saja BRI Liga 1: Duel Lawan PSS Sleman Sangat Krusial, PSIS Semarang Punya Persiapan Spesial 3 Catatan Menarik yang Mungkin Luput dari Laga Persik Vs Persebaya 23 Feb 2025 08:30:00 GMT?.css-1txiau5-AnswerContainer{color:var(--GlobalColorScheme-Text-secondaryText2);}PSM Makassar won 1–0 over Persija Jakarta on Sun Predicted lineups are available for the match a few days in advance while the actual lineup will be available about an hour ahead of the match The current head to head record for the teams are PSM Makassar 2 win(s) Haven't kept a clean sheet in 5 matches Have scored 10 goals in their last 5 matches Persija Jakarta haven't lost to PSM Makassar in their last 12 meetings (5W, 7D). 23 Feb 2025 08:30:00 GMT?PSM Makassar won 1–0 over Persija Jakarta on Sun 23 Feb 2025 08:30:00 GMT.InsightsHave scored 5 goals in their last 5 matches Persija Jakarta haven't lost to PSM Makassar in their last 12 meetings (5W PSM Makassar is playing home against Persija Jakarta at Batakan Stadium on Sun Sign InJoinPrediksi Borneo FC Vs PSM Makassar di BRI Liga 1: Menjaga Asa Tembus 5 Besar | OneFootballBola.com Jakarta - Duel seru yang mempertemukan Borneo FC kontra PSM Makassar akan tersaji di Stadion Segiri Jumat (18/4/2025) sore WIB dalam lanjutan BRI Liga 1 2024/2025 pekan ke-29 Kedua tim mengemban misi berbeda dalam laga kali ini Borneo FC bertekad kembali ke jalur kemenangan seusai bermain imbang 2-2 lawan Persib Bandung (11/4/2025) mereka juga tak mau malu di hadapan suporter sendiri Sementara PSM ingin melanjutkan tren positif setelah menang 2-0 atas Semen Padang pada pekan lalu Tambahan tiga angka penting untuk mengatrol posisi di klasemen bisa dipastikan kedua kesebelasan akan sama-sama ngotot untuk bisa memetik kemenangan 10 Apr 2025 08:30:00 GMT?.css-1txiau5-AnswerContainer{color:var(--GlobalColorScheme-Text-secondaryText2);}PSM Makassar won 2–0 over Semen Padang on Thu The current head to head record for the teams are PSM Makassar 3 win(s) Have scored 5 goals in their last 5 matches 10 Apr 2025 08:30:00 GMT?PSM Makassar won 2–0 over Semen Padang on Thu 10 Apr 2025 08:30:00 GMT.InsightsHave scored 7 goals in their last 5 matches PSM Makassar is playing home against Semen Padang at Batakan Stadium on Thu 25 Apr 2025 12:00:00 GMT?.css-1txiau5-AnswerContainer{color:var(--GlobalColorScheme-Text-secondaryText2);}Bali United FC won 1–0 over PSM Makassar on Fri The current head to head record for the teams are PSM Makassar 6 win(s) Have scored 4 goals in their last 5 matches 25 Apr 2025 12:00:00 GMT?Bali United FC won 1–0 over PSM Makassar on Fri 25 Apr 2025 12:00:00 GMT.InsightsHave scored 8 goals in their last 5 matches PSM Makassar is playing home against Bali United FC at Batakan Stadium on Fri non-commissioned officer in the Indonesian Military (TNI) is in hot water after he and several other men in military uniform allegedly brandished firearms in public in an apparent attempt to threaten a politician in Makassar tried to forcibly enter the home of Harmansyah an executive of the South Sulawesi branch of the Gerindra Party The incident was captured on CCTV footage and quickly went viral on social media the men can be seen waving pistols in front of Harmansyah’s home In a video statement made by Harmansyah’s wife Reni who was at home with her two children during the incident she said that the men came to her house because they wanted to meet with Harmansyah She described how Arifuddin and his accomplices tore down her house’s fence and went right up to the front door demanding to meet her husband Reni also said that Arifuddin threatened to harm and shoot Harmansyah if they could not meet Harmansyah there and then Delivered straight to your inbox three times weekly this curated briefing provides a concise overview of the day's most important issues covering a wide range of topics from politics to culture and society Please check your email for your newsletter subscription Hasanuddin Military Command (Kodam Hasanuddin) in Makassar said that it had opened an investigation and would summon Arifuddin along with his two accomplices for questioning Quickly share this news with your network—keep everyone informed with just a single click Customize your reading experience by adjusting the text size to small or large—find what’s most comfortable for you MAKASSAR, SOUTH SULAWESI — The people of South Sulawesi showed global humanitarian solidarity to commemorate the one-year anniversary of the genocide in Palestine on Sunday (10/6/2024). Through a Tabligh Akbar organized by Dompet Dhuafa South Sulawesi and the Student Executive Board (BEM) of the Faculty of Islamic Religion (FAI) of Universitas Muslim Indonesia (UMI) the solidarity action took place openly in Al-Jibra Auditorium and was attended by various groups-students to public figures The event with the theme “Commemorating the 1st Anniversary of Al-Aqsa Storm in Palestine” aimed to voice support and remember the events that befell the Palestinians participants were invited to reflect on the suffering of the Palestinian people due to prolonged conflict Tausiah delivered by Ustaz Icuk Rifai Al-Azhary also strengthened the spirit of humanitarian solidarity Read also: Commemorating the Prophet&#8217;s Birthday, Dompet Dhuafa Holds Grand Tabligh and Tattoo Removal at Class IIA Cilegon Prison emphasized the importance of continuing to voice support for Palestinian independence invited the public to see the Palestinian issue as a universal humanitarian problem Ramayani emphasized that the genocide in Palestine is not just a religious or racial issue “This event is a reflection that a year ago the genocide carried out by the Zionists was very cruel and claimed many victims to support Palestinian independence,” said Djamal a Palestinian student studying in Indonesia shared the sad story of his family who had to flee due to the conflict His story serves as a reminder of the harsh reality experienced by millions of Palestinians “I hope that the Indonesian people will continue to read and discuss about Palestine which is the independence of Palestine,” he hoped Read also: Welcoming Ramadan, Al Syukro Islamic College Dompet Dhuafa Holds Tabligh Akbar and Mosque Waqf Campaign Dompet Dhuafa’s Disaster Management Center (DMC) representative explained that the DMC team continues to distribute aid through humanitarian missions with the Indonesian government the Indonesian Humanitarian Alliance (IHA) we strive to continue to distribute aid to the Palestinians,” he concluded The event was closed by raising donations and praying together for our brothers and sisters in Palestine led directly by Ustad Icuk Rifai Al-Azhary Dana yang didonasikan melalui Dompet Dhuafa bukan bersumber dan bertujuan untuk pencucian uang (money laundry) maupun tindak kejahatan lainnya yang tidak sesuai prinsip syariah dan regulasi Harap konfirmasi setiap transaksi donasi ke Customer Care Dompet Dhuafa Ririe Ranggasari Petir Garda Bhwana TEMPO.CO, Jakarta - Indonesia's National Commission on Human Rights (Komnas HAM) has called on the Central Java and South Sulawesi police to immediately evaluate how they handled protests and demonstrations in Semarang and Makassar following reports of repressive force by officers during Monday night's protests "We have received reports that authorities used tear gas and even allegedly conducted searches all the way inside a mall," Komnas HAM chairwoman Atnike Nova Sigiro said in a written statement received by Tempo on Tuesday Atnike stressed that the use of excessive force in dealing with demonstrations risks violating human rights particularly the rights to freedom of assembly and expression guaranteed by the Constitution and the Human Rights Law Komnas HAM urged security personnel to refrain from using force and to prioritize a more humane and measured approach to maintaining order Komnas HAM also urged law enforcement officials to guarantee the right of arrested protesters to access legal counsel Denying such access is considered a violation of the right to justice "We also encourage all parties to exercise their fundamental rights responsibly and to maintain a conducive security situation in order to preserve the country's democratic space," she said with a number of government policies sparked the recent wave of demonstrations in Semarang and Makassar anti-Jokowi protests in front of the city hall complex and the Semarang City People's Representative Council (DPRD) were forcibly dispersed by police on Monday Police fired tear gas to disperse the crowd There were also reports that a number of demonstrators A joint demonstration by students from all across Makassar against Joko Widodo's dynastic politics under the Jalan AP Pettarani flyover ended in chaos The use of tear gas and arrests of protesters in Semarang and Makassar have been criticized by various parties There are allegations that the authorities conducted searches in public areas such as shopping malls These repressive measures have been strongly criticized by civil society organizations and academics They believe that the authorities' heavy-handed approach not only violates human rights but also aggravates the situation and threatens democracy The authorities' heavy-handed response to the demonstrations has raised concerns that the space for the expression of aspirations in Indonesia is diminishing especially in light of recent rising political tensions Editor's Choice: Authorities Allegedly Assault Tempo Journalist Covering Jakarta DPR Protest Click here to get the latest news updates from Tempo on Google News Vasectomy for Social Assistance? Dedi Mulyadi's Controversial Proposal Draws Mixed Reactions West Java's Dedi Mulyadi Prepares Rp6bn to Send Troubled Teens to Military Barracks Bareskrim Arrests 4 LPG Agents for Subsidy Fraud, Rp5.6bn in State Losses Prabowo Plans to Build Emergency Bulog Warehouses in Aceh, West Nusa Tenggara Prabowo's Reason for Involving the Military in Food Matters Bareskrim Arrests 4 LPG Agents for Subsidy Fraud How Police Repression Unfolded During Semarang May Day Rally Tempo Journalist Allegedly Assaulted by Police During May Day Rally in Semarang Chaos Erupts at Semarang May Day Rally: Police Arrest Suspected Provocateurs Civil Groups Take to the Streets of Jakarta on May Day: 'This Is the Day to Fight Back' 49 Migrant Workers from NTT Return Deceased Between January and April 2025 Bring Our Activists Home: Greenpeace Indonesia Protests at South Korean Embassy Police Disperse Protest Camp Against TNI Law Before Parliament Building for Third TIme Thousands of Americans Join Protests Against Trump Taman Safari Indonesia Refutes Claims of Circus Worker Exploitation SINGAPORE (2 April 2025) – Buriram United FC will take a two-goal lead into the second leg of their ASEAN Club Championship Shopee Cup™ semi-final against Thai rivals BG Pathum United FC while PSM Makassar of Indonesia hold a slim advantage over Công An Hà Nội FC of Vietnam after Wednesday’s keenly contested first leg clashes Pansa Hemviboon and Guilherme Bissoli earned Buriram United a 3-1 victory over BG Pathum United in Buriram City Stadium while Yuran Fernandes’ header secured a 1-0 win for PSM Makassar against Cong An Ha Noi at the Gelora B.J The teams will reconvene for the second legs in a few weeks’ time with PSM Makassar travelling to Vietnam to take on Công An Hà Nội on 30 April while BG Pathum United will host Buriram United in the return fixture of their all-Thailand affair on 7 May At stake will be a place in the final of the inaugural Shopee Cup™ as the quest to crown the leading team in Southeast Asian football reaches its climax The decider will be played over two legs on 14 and 21 May Buriram United will go into the return meeting with BG Pathum United confident of progressing after a dominant display Osmar Loss’s side taking the lead in the 24th minute when the visiting defence could only clear Peter Zulj’s cross as far as Supachai The Thailand international took aim from just inside the penalty area and after his shot took a huge deflection off the outstretched boot of Nika Sandokhadze the ball looped over Saranon Anuin to put the home side in front Sandokhadze almost made amends six minutes later with a header that hit the post but BG Pathum United did eventually level in the ninth minute of the second half capitalising on a mistake by Buriram United goalkeeper Chatchai Bootprom Gakuto Notsuda’s corner was swung towards the edge of the six-yard box and after Chatchai fumbled his attempted catch Buriram United responded almost immediately with Pansa side-footing in a well-struck volley from Zulj’s corner three minutes later to restore his team’s advantage And the Thai League 1 champions added to their lead with 16 minutes remaining when Bissoli unleashed an unstoppable strike from 25 yards out giving Saranon little chance as he bent the ball into the top corner shaded their meeting with Công An Hà Nội after the visitors squandered the opportunity to take a 15th minute lead PSM Makassar captain Fernandes upended his Công An Hà Nội counterpart Nguyen Quang Hai in the penalty area but goalkeeper Reza Arya pushed Alan Grafite’s spot-kick onto his left post to keep the scores level There was nothing to separate the teams until the 80th minute when Fernandes was given the time and space in the area to meet Victor Luiz’s corner with a header that the central defender powered beyond Filip Nguyen we still have the second match in Vietnam” said Fernandes “I’m very happy to help the team with the goal and now we focus on the second game PSM Makassar v Công An Hà Nội FC – Yuran Fernandes (#4) Buriram United FC v BG Pathum United FC – Guilherme Bissoli (#7) venues and kick-off times for the semi-finals second legs are as follows: SHOPEE CUP™ SEMI-FINAL SCHEDULE The Shopee Cup™ is the first edition of the regional club competition which brings together the leading clubs from across Southeast Asia For more information about the Shopee Cup™, please visit https://aseanutdfc.com/ and @aseanutdfc on Instagram Warius Sampari Wetipo mengatakan KNPB lahir karena militerisme di Tanah Papua yang tidak pernah berhenti sejak 1 Mei 1963 dalam praktiknya operasi militer di Tanah Papua tidak pernah berhenti sejak 1 Mei 1963 ketika bangsa Papua Barat dianeksasi Indonesia Sejak itulah operasi militer secara masif dan terstruktur terjadi di seluruh Tanah Papua Meskipun Pemerintah Indonesia tidak secara resmi mengumumkan penerapan darurat militer di Tanah Papua akan tetapi aparat keamanan terus menerus menggelar berbagai operasi militer di sana “Negara melakukan kejahatan melalui Operasi Trikora Sampai kini kita masih mendengar ada Operasi [Damai] Cartenz Papua adalah zona darurat militer atau Daerah Operasi Militer,” kata Warpo Wetipo saat diwawancarai lebih lanjut melalui layanan aplikasi WhatsApp pada Selasa (28/1/2025) Wetipo menyatakan sejak 2018 terjadi penyisiran di rumah warga penembakan terhadap warga sipil oleh aparat TNI/Polri di berbagai wilayah Papua Pengungsian besar-besaran terjadi di Kabupaten Nduga konflik bersenjata antara aparat TNI/Polri dan kelompok bersenjata Tentara Pembebasan Nasional Papua Barat atau TPNPB terjadi dan menyebar ke berbagai wilayah di Tanah Papua “Konflik bersenjata antara TPNPB dan TNI/Polri terus meluas di berbagai daerah di kita punya tanah air dan terakhir di kabupaten Yalimo dan Tambrauw Rakyat Papua adalah korban utama,” kata Wetipo Pengungsian besar-besaran membuat warga sipil kehilangan rumah Sejumlah orang lanjut usia dan bayi yang meninggal di tengah hutan meninggal Badan Pengurus Pusat BPP KNPB Bersama KNPB Konsulat Wilayah Makassar menggelar seminar nasional bertajuk “Mewujudkan Semangat Perlawanan Rakyat untuk Hak Penentuan Nasib Sendiri bagi Rakyat Papua Barat” di Kota Makassar Seminar itu juga dihadiri perwakilan organisasi kemahasiswaan yang tergabung dalam Kelompok Cipayung Aktivis Front Rakyat Indonesia untuk Pembebasan West Papua (FRI-WP) Wilayah Makassar, Arul mengulas sejarah Presiden Soekarno mengeluarkan Maklumat Trikora di Alun-alun Yogyakarta pada 19 Desember 1961 yang salah satu isinya adalah pembubaran negara boneka Papua buatan Belanda Maklumat itu juga memerintahkan mobilisasi umum yang dipimpin Soekarno “Dampaknya adalah invasi militer besar-besaran di wilayah Irian Barat (West Papua) Kemerdekaan bangsa Papua Barat dikebiri pada 19 Desember 1961,” katanya Menurut Arul konspirasi politik ekonomi Amerika dan Indonesia dalam masa perebutan Irian Barat telah mengorbankan rakyat dan bangsa Papua Barat karena rakyat dan bangsa Papua Barat tidak pernah dilibatkan dalam perundingan itu Nyora mengatakan masalah di Papua Barat itu masalah utama yang dihadap rakyat Papua adalah kolonialisme Indonesia melakukan praktik kolonialisme di Papua Barat Praktik rasisme itu sudah diterapkan sejak awal sejak Bangsa Papua Barat dicaplok masuk ke dalam NKRI,” kata Nyora Nyora membeberkan bangsa Indonesia sebagai kolonial baru merasa dirinya superior terhadap bangsa Papua Dalam setiap keputusan apa pun terkait status politik Orang Asli Papua tidak pernah dilibatkan sebagai subjek Indonesia menganggap bangsa Papua masih terbelakang Kondisi rakyat di Tanah Papua semakin buruk Nyora menyebut Papua secara ekonomi dikuasai oleh kapitalisme asing dan Indonesia hanya menjadi anjing suruhan Kondisi itu membuat masyarakat adat sering mengalami konflik agraria yang menjadi masalah serius dan melahirkan konflik baru “Indonesia hanya menjadi anjing suruhan para kapitalis asing Tanah adat sekalipun dikapitalisasi oleh kaum kapital dan borjuasi lokal,” kata Nyora Rasak menyampaikan bahwa LBH ada untuk mengadvokasi dan perlindungan hukum terhadap para korban terutama kaum lemah yang membutuhkan bantuan hukum LBH Makassar mendampingi para korban dalam berbagai isu termasuk para aktivis Papua yang menyampaikan pendapat dan melakukan protes dalam bentuk demonstrasi “Kami pernah diinterogasi aparat keamanan Indonesia ‘kenapa kalian mengadvokasi isu Papua dan lain sebagainya’ [mereka] dilindungi hak-hak fundamentalnya,” kata Rasak Untuk melihat lebih banyak content JUBI TV, click here and website in this browser for the next time I comment Terverifikasi Administrasi dan Faktual oleh Dewan Pers State construction firm Adhi Karya --also known by its ticker symbol ADHI-- has inaugurated Makassar’s largest hospital which the company hoped could serve the people in the eastern part of Indonesia The Health Ministry Hospital lies at the Center Point of Indonesia in South Sulawesi’s capital of Makassar which was inaugurated by President Joko “Jokowi” Widodo The hospital will serve medical treatment for cancer and brain diseases -- each has its own respective tower The hospital complex also has an eight-story podium a three-story wastewater treatment facility Jokowi even admitted that the hospital’s quality was on par with that of a five-star hotel “The Health Ministry Hospital comes equipped with 920 beds as well as a wide range of modern medical facilities equipped with new technologies The ADHI project also comes with a dock and helipad reflecting the company’s commitment to accessibility and integration with the people’s conditions,” ADHI wrote in its recent press statement ADHI also worked on another state-of-the-art facility namely the Wahidin Sudirohusodo Mother and Children Hospital in Tamalanrea The hospital’s area spans around 43,431.81 square meters and has 8 stories and one semi-basement Facilities include a mother and child polyclinic and radiology rooms There are also rooms for intensive care units (ICU) and a parking building with a capacity of 167 cars “ADHI as a state construction firm is committed to improving the quality of its construction and infrastructure We also wish to complete the projects on time so they benefit the community people can easily access quality health services,” ADHI said The Narcotics Unit of the Makassar Police Department recently busted an international drug ring seizing over 30 kilograms of methamphetamine and thousands of mephedrone pills in coordinated raids across Makassar In a press briefing at Makassar Police Headquarters South Sulawesi Police Chief Inspector General Yudhiawan confirmed the suspects’ ties to an international network operated from an undisclosed Indonesian prison “The operation was controlled by an international network from within a prison,” he said Monday without specifying the facility's location The investigation began with the arrest of two suspects who were found with 26 sachets containing 64 grams of meth on Jalan Opu Daeng Risadju Police later discovered an additional 2 kilograms of meth hidden in a motorcycle compartment The operation then expanded to the Green River View housing complex in Tamalate District Authorities seized 6.219 kilograms of meth in red dragon-emblazoned packages and 8,229 mephedrone pills Interrogations revealed that these substances had been sourced from Kendari The investigation further led police to Alam Salsabilah in Puuwatu District where they apprehended two additional suspects along with two suitcases containing 22 red packages of meth weighing 22.983 kilograms police have issued a wanted list for four other suspects connected to the network alleged to be a recruiter for drug couriers who reportedly acted as operators coordinating drug transactions Authorities reported that the drugs were shipped from abroad before distribution to regions including Makassar The network used secure messaging apps Signal and Zangi to avoid detection The six detained suspects face charges under Indonesia’s Narcotics Law with penalties ranging from six years to life imprisonment or even the death penalty With the seizure of 30.2 kilograms of meth and thousands of mephedrone pills—valued at approximately Rp 50 billion—police estimate that the operation potentially saved 160,000 individuals from harm red-roofed fortress complex on the historic The seventeenth-century Dutch colonial fort does not look out of place in a part of Makassar where Chinese and local South Sulawesi architecture stands alongside glassy Today the Fort is Makassar’s most famous landmark an enduring symbol of tempo doeloe (the olden times) When I first visited Fort Rotterdam last October it was a searing hot day at the end of the dry season and the place was eerily quiet When I returned for the opening night of the 2024 Makassar International Writers Festival (MIWF) in late May this year Someone told me that opening night is always ‘like a giant reunion’ for the local arts community and the public There were rows of food stalls and coloured signs and banners and the main stage was decorated with bright cartoons The festival was established by the late Dr Lily Yulianti Farid through Rumata’ Artspace – a multidisciplinary ‘artspace,’ which she co-founded with prominent film director Riri Riza at the Riza family’s old home in Gunung Sari Baru Although some outside observers perceive MIWF to be a ‘spinoff’ event from Bali’s famous Ubud Writers and Readers Festival (established 2006) MIWF 2024 was the second festival without its founder But her legacy and vision is still felt in creative energies palpable and present there were approximately sixty panels held at MIWF a number that had been steady for some years under the leadership of festival director M that number climbed to 150 programs and activities Running simultaneously with MIWF 2024 was the annual SEAScreen Academy a ‘story camp’ for Southeast Asian filmmakers established by Riri Riza in 2012 creative possibilities to expand the narratives for Eastern Indonesian arts MIWF 2024 opening night began with a dance and musical performance by bissu the fifth androgynous gender in Bugis society that embody both male and female attributes Bissu are considered spiritual mediators between humans and spirits The role of bissu in South Sulawesi life and culture for centuries is celebrated and yet the number of bissu are declining due to escalating LGBTIQ marginalisation in Indonesia By inviting bissu to perform at the festival’s opening night MIWF made an important statement: a gesture both in celebration of South Sulawesi culture and as a show of support amidst increasing threats to their tradition Every evening of the festival a program titled ‘Under the Poetic Stars’ featured on the main outdoor stage the program kicked off with MIWF’s leadership team taking turns to read out poetry by Palestinian poets translated into Bahasa Indonesia – a statement of transnational Similar activist principles are embedded in many other aspects of the festival; including a commitment to transparency this space ‘under the poetic stars’ was given to creatives both emerging and established to showcase a variety of talents these outdoor events created a dialogue between poetics The emphasis on open-air performance is reminiscent of recent history – especially in the early post-New Order period – where Fort Rotterdam was a popular public space for exhibitions and events The reformasi-era Makassar Art Forum ’99 (MAF’99 1999) curated by Solo-based cultural networker and the Journal of Moment Arts (JOMA) performing arts festival (2000-2003) also used this site The use of outdoor space also has an embodied perspective: activating a generous generative conversation in cycle with nature and surrounding environments This experience and connection with the land is especially strong across South Sulawesi’s various cultures and traditions; a point raised often in conversations with artists felt like a continuation of these various ways of storytelling and ‘Othering’ of treating someone as different or 'Other' to oneself they inspired a series of critical discussions This included on feminism and decoloniality; the role of women in filmmaking theatre and literature; and a special performance by Luna Vidya presenting a monologue adaptation of Dapur (Kitchen) responsibility – through which to shape understandings of Eastern Indonesian arts MIWF seems to have responded to this challenge by presenting a feminist space; a forum to nurture emerging perspectives and to empower voices often omitted from Java-centric new art spaces have historically emerged from existing institutions various creative and interdisciplinary initiatives have largely been established in response to the lack of such spaces Rumata’ Artspace and MIWF are positioned in a post-Reformasi-era wave that filmmaking communities and communal libraries established; further raising the profile for Makassar arts What is noticeable now – particularly after the COVID years – is that the emphasis appears to be shifting across Makassar’s arts ecosystem: moving from a preoccupation with making space to generating creative discussions through these channels The impact of this shift is seen at MIWF 2024 but participants also included members of Makassar’s various art networks It is also demonstrated in the types of events organised The festival was primarily interdisciplinary looking beyond the written word to art and photography exhibitions Fort Rotterdam’s various buildings held space for grassroots learning Although these were not necessarily new activities compared to previous years the larger scale achieved this time is notable This interest in promoting visibility through interdisciplinarity has allowed a diverse range of art forms to be represented; in turn feeding back into the larger Makassar arts ecosystem I am sometimes asked whether there is any one theme or ‘flavour’ that might capture Makassar or Eastern Indonesian art for those who have not yet visited It is hard to find an easy answer to this question except to point to the enthusiasm for interdisciplinarity in art-making freed interpretation of art(s) that resists strict categorisation as seni rupa (visual art) It is difficult to answer precisely because it would pinpoint singularity in an area of Indonesia with layers of multiplicity This plural perspective is seen in multi-ethnic multi-cultural Makassar and South Sulawesi and in a broader Eastern Indonesian region with incredible geographic There is still a tendency to see Eastern Indonesian narratives as ‘peripheral’ to Indonesian art and history There are many shifts and vantage points from which to see Indonesian art and Eastern Indonesia has always been important (though overlooked) in this constellation The panels I attended discussed themes including urban issues and experiences it shows the importance of re-centring diversity; and shaping new intra-national and international narratives from this perspective The ’International’ perspectives of MIWF and the ‘Southeast Asia’ of SEAScreen Academy situate Makassar as part of an international this included two ‘Visiting Neighbours’ forums: one for Southeast Asian panellists Bilateral exchange may have their place in these forums the scope of the activities at MIWF positioned Makassar and Eastern Indonesia within a wider set of narratives and sea of islands: looking beyond one place there have been a few speculative conversations about an increase in visibility for contemporary art from Eastern Indonesia within Indonesia at large it looked like a scattered trend was emerging It is why I initially decided to travel to Makassar last year and no doubt will do so again into the future I met with an artist a few days after MIWF It was time to nongkrong (hang out) on my last afternoon in Makassar We were debriefing about the weekend’s events at the festival and my friend remarked that there was a certain energy – a certain momentum – behind MIWF now Observers from afar may talk of a wave of visibility for art from Eastern Indonesia that may be encountered sometime in the distant future What became clear to me at MIWF 2024 is that this ‘wave’ for the arts – plural – is already here It is being shaped by a variety of leading cross-disciplinary storytellers locally and intra-nationally It is also carried through by the work of a younger generation of creative practitioners It is already appearing through principles of activism and access Where this momentum moves next will be watched with keen interest At the closing evening of MIWF I was sitting on the grass ‘under the poetic stars’ when a friend gestured to the blinking warm-bright lights that illuminate Fort Rotterdam by night They asked where else in Indonesia – indeed open venue for a writer’s festival with the historical and environmental resonances that Fort Rotterdam has Caitlin Hughes is a PhD Candidate in Art History and Curatorship at the University of Melbourne Her dissertation studies a history of interdisciplinary art in Indonesia after 1998 through inter-island and international contexts; encompassing Eastern Indonesia as part of this focus Receive Inside Indonesia's latest articles and quarterly editions in your inbox © Copyright Indonesian Resources and Information Program (IRIP) 1983 - 2025 We acknowledge the Traditional Custodians of the stolen lands on which Inside Indonesia is based and we pay our respects to Elders past and present We recognise that sovereignty was never ceded 10 Fakta Tentang Konklaf 2025 Sidang Pemilihan Paus Baru Yang Perlu Anda Ketahui Abu Dhabi Firm to Invest $2bn in Binance Using Trump’s Crypto Venture Trump Family Crypto Venture Tapped As Part Of $2B Emirati-Backed Investment Deal PN Purwokerto Mulai Aktif Pakai Mediator Non Hakim Indonesian Businessman Sues Richard Mille Jakarta Over Undelivered Timepieces Worth $5.3 Million Higher Prices On US Store Shelves Will Coming In Weeks THE EDITOR – Makassar City has made another big achievement at the international level The World Health Organisation (WHO) Southeast Asia Region awarded Healthy City Level 1 Accreditation to Makassar City in recognition of its commitment in realising a sustainable healthy city The award was announced through an official WHO letter dated 9 September 2024 addressed to Makassar Mayor Mohammad Ramdhan Pomanto WHO appreciated the Makassar City Government’s concrete steps in developing short and long term local development plans in accordance with the organisation’s guidelines “We appreciate Makassar City’s serious efforts in addressing the determinants of health and well-being in urban areas and strengthening health governance,” wrote WHO Southeast Asia Regional Director Saima Wazed The accreditation process was conducted through rigorous analyses by WHO and the Regional Laboratory of Urban Governance for Health and Wellbeing The results of the evaluation showed Makassar to be one of the first cities in Southeast Asia to receive this Level 1 accreditation WHO emphasizes the importance of sustaining the efforts that have been made and encourages the Makassar City Government to continue improving the quality of life of the community towards a higher level of accreditation in the next two years WHO will send an award plaque engraved with the name of Makassar City Makassar Mayor Mohammad Ramdhan Pomanto warmly welcomed the award and emphasized that this achievement is the result of the cooperation of all elements of society “This award is clear evidence that our hard work in creating a healthy city has paid off We are committed to continue improving health governance and the welfare of the people of Makassar,” he said WHO also invites Makassar City Government to share their successful experience through written and audio-visual publications on WHO website and social media This aims to inspire other cities in Southeast Asia to follow Makassar’s footsteps in realising a healthier urban environment With this Healthy City Level 1 Accreditation award Makassar has further strengthened its position as one of the pilot cities in Southeast Asia in creating a healthy This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page liputan6 Big match Persija Jakarta kontra PSM Makassar berakhir 1-1 pada lanjutan BRI Liga 1 2024/2025 di Stadion Sultan Agung, Minggu (29/9/2024) malam WIB. Victor Dethan membawa PSM Makassar unggul terlebih dahulu pada menit ke-8 Persija akhinya menyamakan kedudukan melalui Maciej Gajos di menit ke-79 Hasil ini tidak menguntungkan usaha kedua klub memperbaiki posisi di klasemen Liga 1 menempati peringkat 11 dengan torehan sembilan poin Meski juga gagal berjaya dalam jumlah partai sama seperti Persija Juku Eja berada di urutan lima berkat perolehan tiga angka lebih baik Setelah laga ini kompetisi rehat untuk jeda internasional. Persija Jakarta melanjutkan persaingan melawan PSIS Semarang pada 17 Oktober mendatang. Sementara PSM Makassar bersua Madura United sehari setelahnya. PSM Makassar (3-5-2): Hilmansyah; Aloisio Neto, Syahrul Lasinari, Yuran Fernandes; Victor Luiz, Akbar Tanjung, Ananda Raehan, Latyr Fall, Victor Dethan; Abdul Rahman, Tito Okello 15.30 WIB: Bali United vs Barito Putera 3-2 19.00 WIB: Persebaya Surabaya vs Dewa United 0-0 19.00 WIB: Madura United vs Persib Bandung 2-2 15.30 WIB: Semen Padang vs Persis Solo 0-0 19.00 WIB: Persija Jakarta vs PSM Makassar 1-1 Metrics details Adenine base editing can convert sickle hemoglobin (HbS a naturally occurring variant that is clinically asymptomatic the quality and functionality of purified HbG and of mature HbGG and HbGS red blood cells (RBC) has not been assessed we develop a mouse model to characterize HbG Purified HbG appears normal and does not polymerize under hypoxia The topology of the hemoglobin fold with the βΕ6Α mutation is similar to HbA in the oxy and deoxy states showing altered function and increased sickling under hypoxia Blood counts and mitochondrial retention measures place HbGS RBCs as intermediate in severity between HbAS and HbSS while organ function is comparable to HbAS Our results highlight the importance of functionally assessing the mature red cell environment when evaluating novel gene editing strategies for hematologic disorders adenine base editing technology can be used to directly edit the sickle point mutation While the sickle point mutation in the hemoglobin β subunit (βE6V) cannot be directly corrected to wild-type (βE6) by an adenine base editor (ABE) it can be converted to a naturally occurring hemoglobin variant This approach presents a promising alternative base editing option to treat SCD as it has the potential to convert the pathogenic HbS to a non-pathogenic form of hemoglobin of mature RBCs containing HbGS and HbGG have not been described the lack of an HbG protein crystal structure prevents assessing how the βE6A mutation affects the globin protein fold and its function The effect of HbC on HbS can only be observed in the mature RBC; dehydration increases the mean corpuscular hemoglobin concentration (MCHC) increasing the risk of HbS polymerization and amplifying the effect of the 50% HbS contained in these cells to further biologically de-risk a base editing strategy predicated on the direct conversion of HbS to HbG-Makassar it is crucial to understand the impact of both HbGG and HbGS on red blood cell function in the mature RBC we generate transgenic human mouse models expressing human HbGG and HbGS from a well-established Townes mouse model and characterize the purified HbG protein as well as the mature RBC biophysical properties Functional and structural characterization of HbG coupled with RBC functional assays and organ characterization reveal that purified HbG as well as homozygous HbGG RBCs have characteristics resembling that of functional hemoglobins when HbG was present with HbS in an RBC environment HbGS RBCs have a phenotype intermediate in severity between HbAS and HbSS as observed by various hematological indices In addition to highlighting the importance of functionally assessing the mature RBC environment when evaluating novel gene editing strategies for hematological disorders our work provides evidence that supports the direct bi-allelic correction of HbS to HbG using base editing as a viable and promising approach to treat individuals suffering with sickle cell disease Gene architecture of the chromosome 7 locus in the Townes mouse containing Human HBB genes63 Endogenous mouse genes are highlighted with teal bars with their respective gene names Human HBG1 (5675 bp) and human HBB (4113 bp) tandem sequences containing intact human genomic 5′ promoter and 3′ flanking regions highlighted in yellow are replacing murine Hbb-bmaj and Hbb-bmin Intended knock in nucleotide substitutions to generate Makassar β globin (E6A codon underlined) in exon 1 of HBB are highlighted in red The gRNA spacer regions used to generate KI are shown in blue and green synonymous mutations introduced with DNA donor oligo template to prevent re-cutting are indicated in bold black a Oxygen binding affinity comparison between HbA Traces shown represent a single curve of data collected in quadruplicates Numbers shown represent the average and standard deviations of Hill coefficients and p50 values calculated from each individual trace Name in parenthesis indicates source utilized for isolation (Townes-mice blood In vitro polymerization kinetics of purified (b) HbG at various concentrations (c) HbA:HbS and (d) HbG:HbS mixtures at various HbS percentages at a total hemoglobin concentration of 2 mg/mL e The relationship between the log of reciprocal delay time and HbS concentration of binary mixtures of either HbA (blue circles) or HbG (red squares) with HbS is shown Extra sum-of-squares F test was performed to compared both data sets HbS percentages used for each mixture are shown above their respective data points Source data are provided as a Source Data file c Simulated annealing composite omit maps (2Fo-Fc) are contoured to 1.0 σ for the heme cofactor in the HbG-Makassar tetramer Heme cofactor is shown as red sticks and the composite omit map is shown as blue mesh The presence (a) and absence (c) of the electron density for the water molecule coordinated to the heme iron suggest the protein is in its relaxed and tense states d Ribbon representation of superimposed structures of HbG-Makassar and HbA in its R2 (b; HbA PDB id: 1BBB) and T state (d; HbA PDB id:2DN2) The α and β protomers are shown in teal and purple for HbG-Makassar and gray for HbA Hemes are shown as red sticks and the βE6A substitution found in HbG-Makassar is shown as light purple spheres indicating that HbG contributes to red cell dehydration RBC lifespan as suggested by reticulocyte count are similar between HbAA and HbGG at steady state but may be different in a physiologically stressed or dehydrated state coinheritance of a hemoglobinopathy (HbGG or HbSS converted to HbGG by gene therapy) and an otherwise benign RBC condition that impacts RBC hydration (i.e. PIEZO1 mutations) could worsen HbGG lifespan leading to a more severe clinical presentation a Protospacer and PAM (blue) sequence used for base editing approach to target the HbS allele to convert sickle valine to alanine with target adenines highlighted b NGS of non-mobilized (n = 2 donors) and mobilized peripheral blood (n = 1 donor) HbSS CD34+ cells 48 h post-electroporation and d14 in vitro erythroid differentiated (IVED) cells indicating highly efficient Makassar editing and low percentage non-synonymous bystander editing c Globin quantification of relative abundance of Makassar and HbS globins by UHPLC of bulk IVED cultures from (b) Two independent IVED cultures were generated for the mobilized donor and quantified for globin abundance d Allelic breakdown of editing outcomes from single IVED clones derived non-mobilized (n = 2 donor) and mobilized (n = 1 donor) shown in (a) For non-mobilized donor (n = 172 and 128 single cell colonies) and for mobilized HbSS donor (n = 83 single cell colonies) single colonies were analyzed respectively e Globin abundance of bulk (n = 1 donor) and single IVED colonies (n = 18 single cell colonies) of a HbAS donor and mono-allelically edited Makassar HbSS IVED colonies (n = 14 colonies) bulk d18 IVED editing measured at day 18 following IVED Data is presented as mean ± standard deviation our ex vivo editing data suggests that installation of the HbG-Makassar variant can be achieved in CD34+ cells with high efficiency and can lead to >90% of red blood cells that no longer express HbS protein or achieve therapeutically relevant reduced levels of HbS There has been significant progress in gene-edited cell therapy options for individuals with SCD making “one-and-done” therapies a possibility HbF induction through disruption of the BCL11A erythroid enhancer element is an FDA approved gene editing strategy providing clear benefit for patients receiving this therapy Induction of HbF through various alterations of the gamma globin promoter are also in clinical trials with the potential to correct the sickle mutation to wild-type either HbF or HbA; taking advantage of the vast literature available on the effect of these hemoglobins in a mature RBC when present with HbS This observation suggests that the β6A mutation in HbG is not prone to make additional contacts in polymers being formed by hybrid HbGS mixtures Our studies with purified HbG suggests that the benefits of having the βE6A substitution in the Makassar variant in mitigating hemoglobin polymerization does not come at the expense of affecting its functional parameters when it comes to oxygen binding thus providing a direct benefit to SCD patients HbSS individuals also have increased %DRBC but this may be due to a different mechanism of gradual dehydration due to cycles of sickling and unsickling older RBCs are denser; this is why in the mouse model HbSS RBC are not dense; RBC lifespan is shorter than in the human The rheological properties of HbGG RBCs resembled that of HbAA RBCs except for metrics affected by dehydration Although the glomerular sclerosis score demonstrated a rising trend across genotypes with the highest score in HbSS differences did not achieve statistical significance likely due to the small sample size (N = 3) we hypothesize that the intermediate red cell function (between HbAS and HbSS) that we observe in HbGS is due to dehydration of the RBC A direct comparison between a HbGS and HbSC mouse RBC was not possible based on our experience with human HbSC rheology it appears that HbGS RBC are better quality than that of HbSC; possibly due to less dehydration the HbGS state represents a less frequent (<15%) editing outcome with bi-allelic editing by far predominating (>70%) A minor population of RBCs are likely to have a 60:40 ratio of HbG:HbS and even these RBCs will exhibit significant functional improvement over the original HbSS RBCs we expect to significantly improve the sickle cell phenotype in >90% of cells with a base editing approach the Makassar direct replacement strategy of pathogenic HbS offers another promising therapeutic option for patients with sickle cell disease The research presented in this manuscript complies with all relevant ethical regulations All mice were maintained and studied according to the National Institute of Health Guide for the Care and Use of Laboratory Animals following an approved protocol 202000101 by Emory University Institutional Care and Use of Animals Committees Mobilized peripheral blood stem cell (PBSC) from Sickle Cell Disease (SCD) patients were collected under BCH protocol IRB-P00034825 Written informed consent was obtained for research purposes only Non-mobilized HbSS CD34+ cells were obtained under an Emory approved IRB protocol IRB00034535 for research use in the present project and written informed consent was provided by the donors All experimental mice used in this study were bred in-house at Emory and genotypes (HbAA, HbAS, HbSS, HbGS, or HbGG) were verified using Sanger sequencing (Genewiz). Supplemental Table 5 contains the numbers of mice and statistical analyses used for each figure Each mouse was weighed at the time of sacrifice and euthanized via cervical dislocation using isoflurane anesthesia and liver) were collected to characterize hematology and rheology Mice could not be randomized to experimental groups but experimenters were blinded to genotype during the experimentation and analysis HbGS genotypes were subsequently generated by crossing HbAG to HbSS and HbAS Townes mice Genotypes were determined by Sanger sequencing The mice were group-housed in individually ventilated cages on corncob in a barrier facility given ad libitum reverse-osmosis–purified chlorinated water and irradiated rodent diet (LabDiet: 5053 - PicoLab® Rodent Diet 20) and maintained on a 12:12-h light: dark cycle The intact mass and sequence identification of purified hemoglobin proteins were determined by Liquid Chromatography-Mass Spectrometry to verify its molecular identity Purified proteins (n = 1) were diluted to 0.5 mg mL-1 and analyzed on a UHPLC system with a binary pump equipped with a 2.1 × 150 mm C18 column Mobile phase A was 0.1% v trifluoroacetic acid (TFA) in water and mobile phase B was 0.08% v TFA in acetonitrile Mass detection was performed with an in-line Q Exactive HF mass spectrometer (Thermo Fisher Scientific) acquiring intact mass data in the range from 500 to 2000 Da Spectrum was analyzed using the BioPharma FinderTM software (Thermo Fisher Scientific Oxygen equilibration curves were analyzed using the HEMOX analyzer data software yielding p50 values (partial pressure of oxygen at which hemoglobin is half-saturated) and n50 (Hill coefficient) Values reported for the p50 and Hill coefficients consist of the average and standard deviation of 4 individual measurements Polymerization assays were performed as previously described33 100 μL of sample at various concentrations (2 0.5 mg mL-1) in polymerization buffer (1.8 M potassium phosphate buffer 5 mg mL-1 sodium dithionite) was dispensed on a pre-chilled 96 well plate The plate was then loaded onto an Omega Spectrostar plate reader coupled with an atmospheric-controlled unit where hypoxic conditions (<1% oxygen) were kept by flushing the instrument with nitrogen gas Polymerization delay times were measured by monitoring the optical density of the samples at 37 °C and at a 700 nm wavelength as a function of time Polymerization of hemoglobin mixtures was performed as described above with slight modifications HbAS and HbGS mixtures of hemoglobins were made at a constant 2 mg mL-1 total hemoglobin with HbS ratios of 0% Mixtures were made in polymerization buffer and polymerization delay times were measured as described above All crystallization conditions were prepared and optimized using a Mosquito robot (SPT Labtech) at 20 °C drops were prepared by mixing 0.5 μL of HbG isolated from Townes mice (13 mg mL-1 in 20 mM Tris-HCl pH 7.5 and 1 mM TCEP) and 0.5 μL of reservoir solution (0.1 M TRIS pH 8.0; 26-36% (v/v) PEG 6,000) and equilibrated against 70 μL of reservoir solution The crystals were transferred to a cryoprotectant solution (0.1 M Tris pH 8 18% glycerol) and flash-cooled in liquid nitrogen the crystallization condition was identified and optimized in an MBraun anaerobic glovebox Drops were prepared by mixing 0.5 μL of recombinant HbG isolated from E and 20 mM sodium dithionite) and 0.5 μL of reservoir solution (0.055 M Citric acid and equilibrated against 70 μL of reservoir solution The crystals were transferred to a cryoprotectant solution (0.055 M Citric acid was used for all rheological devices and measurements Complete blood counts were obtained using hematology analyzers Element HT5 (HESKA RBCs were stained with Hoechst 33342 and subjected to 2% sodium metabisulfite (MBS) by volume as previously described58 Images were collected by light microscopy at baseline and every minute for thirty minutes post-MBS addition reported as a percentage of the total were quantified by two individuals blinded to the RBC genotype Viscosity was measured using a Beckman cone and plate viscometer An aliquot of 500 μL of whole blood was run through a multipoint viscosity test starting at 6 rpm with a shear rate of 45 (1 s-1) then raised to 30 rpm at a shear rate of 225 (1 s-1) The two average values of viscosity across those conditions were then recorded and the hematocrit to viscosity ratio (HVR) calculated As viscosity rises past a transition point typically an Hb of 15 in a healthy individual oxygen carrying capacity begins to be impaired due to decreased blood flow viscosity rises more precipitously with increase in Hb than in a healthy individual; however the HVR is more informative than whole blood viscosity; higher HVR is favorable indicating less viscosity rise with increase in Hb/hematocrit an aliquot of 5 μL of EDTA anticoagulated whole blood was washed once with staining buffer (Dulbecco’s Phosphate-Buffered Saline without Ca2+ and Mg2 +  Blood was centrifuged at 500 x g for 5 minutes at room temperature and supernatant was discarded Red blood cells were stained in 100 μL of 300 nM MitoTracker Deep Red (Invitrogen cat# M46753)) and 0.4 μg of TER119-BUV395 (BD Biosciences Cat# 563827) in staining buffer Cells were incubated in the dark for 45 min Cells were then fixed in 0.4% PFA on ice for 10 minutes before washing three times The flow panels were run on a FACSymphony A5 and A3 flow cytometers Analysis was then performed through FlowJo v10 software 1 × 10^6 cells were washed in 1× Annexin Binding Buffer (Biotium Cat# 99902) Dead cells were identified using the Live/Dead Fixable Near IR (780) Viability dye at a 1:1000 dilution Samples were incubated at room temperature for 15 min cells were stained with TER119-BUV395 (0.4 μg/106 cells) and CD44-APC (0.35 μg/106 cells) were added and incubated for an additional 20 min in the dark Cells were washed twice with 1× Annexin Binding Buffer and cells were incubated in 100 μL for 15 min in the dark Samples were analyzed on the cytometer within one hour of staining for Annexin Analysis was performed using FlowJo v10 software Sample size was derived using data from a pilot sample of three mice PCR amplification of the mRNA template was used in subsequent in vitro transcription The NEB HiScribe High-Yield Kit was used as per the instruction manual but with full substitution of N1-methyl-pseudouridine for uridine and co-transcriptional capping with CleanCap AG (Trilink) Reaction cleanup was performed by lithium chloride precipitation Electroporations were conducted with 50 nM of in vitro transcribed mRNA encoding base editor IBE12.2 and 5 μM of sgRNA targeting the HbS allele in 25 µL final volume reactions Mobilized peripheral blood CD34+ cells from individuals with SCD were generously provided by the Manis Lab (BCH) Non-mobilized HbSS CD34+ cells were obtained from red cell exchange bags collected under an Emory approved IRB protocol for research use in the present project and written informed consent was provided by the donors Peripheral blood mononuclear cells (PBMCs) were isolated using density centrifugation by Ficoll-Paque (GE healthcare) per manufacturer’s protocols of apheresis product RBCs were removed using GlyA (StemCell Technologies RBC depletion kit); CD34+ cells were isolated by magnetic separation with UltraPure human CD34+ positive enrichment kit with LS columns (Miltenyi Biotech) hCD34+ cells were pelleted and resuspended in Iscove’s Modified Dulbecco’s Medium (IMDM; ATCC) containing 5% human serum 5 μg mL-1 IL3 and 50 μM hydrocortisone (Sigma) at 2 × 104 cells per mL cells were pelleted and resuspended in the same IMDM media without IL3 and hydrocortisone at a concentration of 2 × 105 cells per mL cells were pelleted and resuspended in IMDM medium containing without SCF at 1 × 106 cells per mL the cells were pelleted and resuspended in the same IMDM medium at 5 × 106 cells per mL Differentiated red blood cells were collected on day 18 for UHPLC processing Single cell IVED clones were obtained by limiting dilution of CD34+ cells 48 h post-electroporation into 96 well U-bottom plates that were confirmed by NGS to be the genotypes of interest UHPLC analysis was performed as previously described15 The separation conditions were further optimized for the separation of HbG from HbS A reverse-phase column at a temperature of 75 °C was used Mobile phases were 0.1% v trifluoroacetic acid (TFA) in water (A) and 0.08% v TFA in acetonitrile (B) with a flow rate of 0.25 mL min-1 A gradient of 38–48%B 0–23 min; 48–99%B 10–23–26 min; and 99–38%B to 26–28 min was applied UV spectra at a wavelength of 220 nm with a data rate of 5 Hz were collected throughout the analysis Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article Polymerization in erythrocytes containing S and non-S hemoglobins Hemoglobin S polymerization: primary determinant of the hemolytic and clinical severity of the sickling syndromes Life expectancy and risk factors for early death Factors associated with survival in a contemporary adult sickle cell disease cohort Long-term survival with sickle cell disease: a nationwide cohort study of Medicare and Medicaid beneficiaries Defining curative endpoints for sickle cell disease in the era of gene therapy and gene editing Efficacy and safety of recently approved drugs for sickle cell disease: a review of clinical trials Transfusion therapy for sickle cell disease: what’s new Allogeneic donor availability for hematopoietic stem cell transplantation in children with sickle cell disease HLA haploidentical stem cell transplant with pretransplant immunosuppression for patients with sickle cell disease Haploidentical peripheral blood stem cell transplantation demonstrates stable engraftment in adults with sickle cell disease Gene therapy for sickle cell disease: moving from the bench to the bedside Curative options for sickle cell disease: haploidentical stem cell transplantation or gene therapy Potent and uniform fetal hemoglobin induction via base editing Directed evolution of adenine base editors with increased activity and therapeutic application Hemoglobin G Makassar: beta-6 Glu leads to Ala Molecular basis and hematological features of hemoglobin variants in Southern Thailand Hemoglobin variants in northern thailand: prevalence heterogeneity and molecular characteristics Clinical and haematological characteristics of 38 individuals with Hb G-Makassar in Malaysia Hb G Makassar (beta 6:Glu-Ala) in a Thai family Base editing of haematopoietic stem cells rescues sickle cell disease in mice Enhanced polymerization of recombinant human deoxyhemoglobin beta 6 Glu–Ile Polymerization and solubility of recombinant hemoglobins alpha 2 beta 2 6 Glu->Ala (Hb Makassar) and alpha 2 beta 2 6 Glu->Ala Steric and hydrophobic determinants of the solubilities of recombinant sickle cell hemoglobins Effects of beta 6 amino acid hydrophobicity on stability and solubility of hemoglobin tetramers Human hemoglobin G-Makassar variant masquerading as sickle cell anemia A new inherited abnormality of human hemoglobin The hematologic effects of hemoglobin C alone and in combination with sickle cell hemoglobin Hemoglobin C: report of the homozygous condition and of combinations with normal and sickle cell hemoglobin Human sickle hemoglobin in transgenic mice Rationally designed base editors for precise editing of the sickle cell disease mutation Nucleation-controlled aggregation of deoxyhemoglobin S Possible difference in the size of nuclei in different phosphate concentrations A third quaternary structure of human hemoglobin A at 1.7-A resolution The high resolution crystal structure of deoxyhemoglobin S Automated quantitation of cell density distribution and hyperdense cell fraction in RBC disorders Kinetics of sickle cell biorheology and implications for painful vasoocclusive crisis Erythrocyte density in sickle cell syndromes is associated with specific clinical manifestations and hemolysis Biological parameters predictive of percent dense red blood cell decrease under hydroxyurea Sickle-cell haemoglobin polymerization: is it the primary pathogenic event of sickle-cell anaemia Density ultracentrifugation of sickle cells during and after pain crisis: increased dense echinocytes in crisis Pathophysiological characterization of the Townes mouse model for sickle cell disease Hemoglobin CC disease: rheological properties or erythrocytes and abnormalities in cell water Cell volume regulation in hemoglobin CC and AA erythrocytes Comparison of mechanisms of anemia in mice with sickle cell disease and beta-thalassemia: peripheral destruction and phospholipid scramblase-mediated phosphatidylserine exposure Blood rheology biomarkers in sickle cell disease Rheological properties of sickle erythrocytes in patients with sickle-cell anemia: The effect of hydroxyurea Separation of hemoglobin variants by ion-exchange chromatography on Monobead resins Production of unmodified human adult hemoglobin in Escherichia coli Purification of hemoglobin from red blood cells using tangential flow filtration and immobilized metal ion affinity chromatography An enzymic reduction system for metmyoglobin and methemoglobin and its application to functional studies of oxygen carriers How good are my data and what is the resolution 1.25 A resolution crystal structures of human haemoglobin in the oxy PHENIX: a comprehensive Python-based system for macromolecular structure solution Highly efficient therapeutic gene editing of human hematopoietic stem cells Oxygen gradient ektacytometry-derived biomarkers are associated with vaso-occlusive crises and correlate with treatment response in sickle cell disease Retention of functional mitochondria in mature red blood cells from patients with sickle cell disease Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells Hyperfiltration predicts long-term renal outcomes in humanized sickle cell mice Knockout-transgenic mouse model of sickle cell disease Download references and Blood Institute at the NIH for providing the expression plasmids for recombinant hemoglobin and Damien Fenske-Corbiere from Beam Therapeutics for NGS support The authors thank the Drennan Lab at MIT for providing access to equipment needed to perform anaerobic crystallographic studies This research used resources of the National Synchrotron Light Source II; a U.S Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Brookhaven National Laboratory under Contract No The Center for BioMolecular Structure (CBMS) is primarily supported by the National Institutes of Health National Institute of General Medical Sciences (NIGMS) through a Center Core P30 Grant (P30GM133893) and by the DOE Office of Biological and Environmental Research (KP1605010) These authors contributed equally: Zachary Kostamo performed biochemical and structural studies performed ex vivo primary human CD34 isolation editing and downstream in vitro experiments performed all LC-MS and UHPLC globin analytic assays supervised biochemical and structural work supervised the project and reviewed manuscript designed the red cell function and mouse pathology experiments All authors from Beam Therapeutics disclose a conflict of interest and are shareholders of Beam Therapeutics The remaining authors declare no competing interest Nature Communications thanks the anonymous reviewers for their contribution to the peer review of this work Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41467-025-56578-3 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology Cari English The Clean Water Crisis That Makes North Makassar Miserable TEKS › English›The Clean Water Crisis That.. Iklan The Clean Water Crisis That Makes North Makassar Miserable The clean water crisis is a problem for some residents of Makassar City Audio Berita This article has been translated using AI. See Original Please note that this article was automatically translated using Microsoft Azure AI, Open AI, and Google Translation AI. We cannot ensure that the entire content is translated accurately. If you spot any errors or inconsistencies, contact us at hotline@kompas.id and we'll make every effort to address them TEKS KOMPAS/RENY SRI AYU ARMANCondition of the spillway building at Leko Pancing Weir Currently the weir is dry due to the drought clean water is a scarce commodity for some residents of Makassar City clean water provided by the local water company cannot reach all areas The clean water crisis often arrives before the dry season and causes residents to suffer It was late afternoon on Monday (10/6/2024) when Hajrah (45) was sitting on the edge of a alley in Sabutung 1 Street She had just finished buying water from a roaming water vendor Clean water is bought from vendors who roam around carrying carts filled with 5-20 liter jerrycans The price is Rp 5,000 for one cart filled with 10 five-liter jerrycans or Rp 5,000 for six 20-liter jerrycans "Every day I have to set aside 10,000-20,000 rupiahs for water expenses for buying water can swell," he said A cart loaded with jerrycans was seen in one of the alleys on Sabutung 1 Street The water crisis in this area has forced residents to fulfill their need for clean water by purchasing it spends at least Rp 30,000 per day to buy clean water The number of people in her house is more numerous Every day I have to set aside 10,000-20,000 for water The water sold is generally clean water produced by the local water company Some lucky homeowners who receive consistent water supply from the company use this as a source of income It is from these homes that traders buy water and then sell it again Also read: The Water Crisis in Makassar is Getting Worse sometimes they are directly connected to the homeowner's pipes and we pay per hour Usually it's Rp 30,000 or Rp 40,000 per hour I also often connect it to a neighbor's house where the water happens to flow," said Jumriah It is not surprising to see water hoses stretching from house to house in the northern part of Makassar especially in the Ujung Tanah and Tallo districts It is also common to see carts filled with jerrycans in the alleys and settlements Condition of the Bili-Bili dam in Gowa Regency This dam is a source of raw water for PDAM Makassar acknowledged that the water crisis in this area has been happening for a long time He calculated that residents have been experiencing this type of situation for almost 20 years PDAM water only flows for around 4-5 months when there was no water flow from before Eid until the end of the year," he said This means that this year's water crisis will last around 6-7 months It's really difficult being a person in the northern region," he said are not without efforts to find sources of clean water apart from the PDAM "The problem in the North is that there are very few options for clean water the water is fresh but its color is like fish water (murky) the water is salty like seawater," he said Also read: Water is difficult and electricity is out in Makassar The condition of the Jeneberang River in Gowa Regency on Friday (7/6/2024) is in disarray due to rampant mining activities for coal and stones along the river's banks Director of PDAM Makassar Beni Iskandar admitted that parts of the northern region of Makassar are still experiencing a clean water crisis at least 20 percent experience a clean water crisis every year Problems ranging from old pipes to installing illegal pipes into people's homes are the reasons. Not to mention the raw water supply at Leko Pancing Dam which is often stretched due to sharing with rice field irrigation water illegal connections to residents' fields are also common Even though irrigation channels have been regulated and divided Bendung Leko Pancing was only intended for raw water needs of the local water company it has also been regulated for field irrigation there are many illegal connections for fields,” he said which is one of the raw water sources for the Makassar PDAM namely damage in the upstream and the river basin areas the dryness of the Leko Pancing River even caused the Leko Pancing Dam to be unable to produce clean water only 300 liters per second remain out of the normal capacity of 500 liters per second A visitor walks in the pine forest in Lembanna The forest is located at the foot of Mount Bawakaraeng where the headwaters of Jeneberang River is located Another raw water source for Makassar's water supply company PDAM is the Bili-Bili Dam This dam utilizes the Jeneberang River as its main water source The upstream of this river is located in the Gunung Bawakaraeng and Gunung Lompobattang areas Also read: Feast of Ideas from the World for Sustainable Water the upstream area of Jeneberang also experiences degradation In the Tinggimoncong and Tombolo Pao districts of Gowa Regency The upstream condition worsened after a major landslide in the caldera of Mount Bawakaraeng in 2004 Landslides also cause sediment to enter the Bili-Bili Dam at any time Sand and stone mining along the Jeneberang River has also changed the river's form the Bili-Bili Dam experienced a significant decrease in water levels The siltation has made the water unable to pass through the spillway structure Last year's prolonged drought resulting in a water crisis is no longer limited to Northern Makassar but is now affecting the majority of the city The Makassar PDAM and Damkar have intervened by supplying water to residential areas using tanker trucks Visitors are enjoying the Takapala waterfall in Tinggimoncong District with several rivers including the Jeneberang River serves as a source of raw water for the Bili-Bili Dam PDAM is currently making an intake in the Moncongloe River and also utilizing the flow of the Tallo River which divides Makassar "The work is currently in progress and we hope it can be completed soon and utilized As for the connection of illegal irrigation to residents' rice fields we have also communicated with the river bureau responsible for it," said Beni the Mayor of Makassar stated that he would try to search for groundwater sources by utilizing a geoelectric system "We will try to find points where there is still usable groundwater," he said The Leko Pancing Dam is temporarily not operating due to receding river water as seen on Wednesday (30/8/2023) The issue of water should actually be addressed across sectors and involve neighboring Makassar district government but also other parties related to land opening and mining issues the water crisis is likely to continue to worsen Also read: Merging Reservoirs and Rivers Juara bertahan Persib Bandung gagal kembali ke jalur kemenangan di BRI Liga 1 2024/2025. Mereka harus puas bermain tanpa gol melawan tuan rumah PSM Makassar di Stadion Batakan, Rabu (11/9/2024). Mereka harus puas bermain tanpa gol melawan tuan rumah PSM Makassar di Stadion Batakan Kedua tim gagal menembus pertahanan lawan dengan amunisi masing-masing Juku Eja memiliki Nermin Haljeta yang berstatus top skor sementara kompetisi Sementara walau David Da Silva masih absen Pangeran Biru punya Ciro Alves dan Dimas Drajad Hasil ini tidak banyak mengubah posisi kedua tim di klasemen sementara Liga 1. PSM masih di puncak dengan koleksi 10 angka hasil empat pertandingan. Namun, Juku Eja rawan disalip rival. Sementara Persib berada di urutan tujuh tabel. Pasukan Bojan Hodak baru memiliki enam poin usai gagal berjaya di tiga pertandingan terakhir. PSM selanjutnya dijadwalkan menghadapi Arema FC, Minggu (15/9/2024). Sementara Persib bertemu PSIS Semarang di hari yang sama. Di Stadion Kapten I Wayan Dipta, Bali United urung memanfaatkan faktor kandang untuk mengalahkan Arema FC. Laga ini berakhir tanpa gol. 15.30 WIB: Dewa United vs PSIS Semarang 2-1 15.30 WIB: PSM Makassar vs Persib Bandung 0-0 19.00 WIB: Persita Tangerang vs Persebaya Surabaya Sign InJoinBRI Liga 1: Bernardo Tavares Bicara Peluang PSM Makassar Gaet Pemain di Bursa Transfer Paruh Musim | OneFootballBola.com Bola.com, Jakarta - Putaran pertama BRI Liga 1 2024/2025 tinggal menyisakan tiga pertandingan masih ada tiga partai lagi yang akan dimainkan di pengujung tahun ini Menurut jadwal, pekan ke-15 hingga ke-17 berlangsung pada 14-29 Desember 2024. Sementara itu, bursa transfer BRI Liga 1 mulai dibuka pada 19 Desember 2024 sampai 15 Januari 2025 persaingan di papan klasemen kasta tertinggi sepak bola Indonesia itu pun kian seru PSM Makassar untuk sementara berada di peringkat keenam dengan koleksi 23 poin Tim berjulukan Juku Eja itu ada dibawah Persebaya Surabaya yang mengemas 33 poin Airport Dimensions is expanding into Indonesia through a strategic 45 per cent investment stake in Blue Sky Group with a new lounge opening later this year as a result of the partnership The new airport lounge at Makassar Sultan Hasanuddin International Airport is set to open later this year and is the first project under this collaboration Blue Sky Lounge already operates various premium airport lounges in key Indonesian airports including Soekarno-Hatta International Airport in Jakarta Ngurah Rai International Airport in Bali and Juanda International Airport in Surabaya the partnership will see Airport Dimensions welcome a further 11 airport lounges to its network in regions and airports such as Jakarta Driven by rising tourism and increasing foreign investment “Indonesia represents an incredibly dynamic market and we are thrilled to partner with Blue Sky Lounge to bring our global expertise to this rapidly growing aviation network,” said Errol McGlothan President EMEA and APAC at Airport Dimensions Describing the region as the second-fastest growing aviation market in the world McGlothan added that “Indonesia offers immense opportunities” He also said that the new lounge at Makassar “signifies [Airport Dimensions’] commitment to elevating the passenger experience and we look forward to seeing how this will transform the airport lounge experience in the region.” Airport Dimensions will work closely with Blue Sky Lounge and local stakeholders to deliver high-quality innovative lounge experiences that are perfectly tailored to the local environment and culture added that the partnership “marks a significant milestone in our over 50 years of journey in this hospitality industry in the country We hope to bring greater travel experience into all airports throughout Indonesia and we hope that through this partnership we can expand our presence internationally.” Image source: Airport Dimensions – Signing ceremony Airport Dimensions EMEA President Errol McGlothan and Blue Sky Group President Sign InJoinLink Live Streaming PSM Makassar Vs Persik Kediri di BRI Liga 1 | OneFootballBola.com Bola.com, Jakarta - Link live streaming PSM Makassar versus Persik kediri pada lanjutan BRI Liga 1 2024/2025 Pertandingan ini dapat disaksikan melalui siaran langsung di Indosiar dan live streaming di Vidio PSM datang bermodal catatan tak terkalahkan dalam empat pertandingan terakhir di Liga 1 Mereka menang dua kali dan imbang juga dua kali PSM bertengger di peringkat kelima dengan raihan 16 poin dari sembilan pertandingan Posisi PSM jelas lebih baik daripada Persik Saat ini, Persik menempati peringkat ke-10 klasemen BRI Liga 1. Namun, jarak dengan PSM Makassar tidak terlalu jauh karena kedua tim hanya dipisahkan dengan dua poin Related NewsBarcelona hand trial to teenage Brazilian midfield prospect How soon can Manchester City secure UEFA Champions League qualification this season? “Good on you, man!” – Alan Shearer backs Chelsea player after controversial incident Arsenal suffer a blow ahead of Champions League semi-final tie with PSG Newcastle United star has been nominated for PFA award and the Makassar City Government of Indonesia formally signed the Concession Agreement for the Makassar Waste-to-Energy Project Assistant Deputy Minister of Energy of Maritime and Investment Coordination Ministry as well as leaders from the Makassar Environmental Department "The implementation of this project represents a pivotal advancement in Indonesia's transition to a clean energy future The introduction of advanced waste treatment technology will not only help Indonesia address its municipal waste issues but also contribute to optimizing the country's energy structure." Chief Technology Officer of SUS ENVIRONMENT "The Makassar Waste-to-Energy Project is not only a crucial component of SUS Environment's global strategic blueprint but also a significant milestone in Indonesia's march towards a green energy era and the innovation of its waste management system."  He further emphasized that SUS would leverage its technological and managerial strengths to ensure the simultaneous achievement of environmental working alongside various sectors in Indonesia to promote environmental protection and contribute to the well-being of local communities and global environmental improvement the capital of South Sulawesi Province in Indonesia has a construction scale of 1,300 t/d and is equipped with 2 x 650 t/d incineration lines and a 1 x 35MW turbine & generator.  The project is expected to commence construction in 2024 with an estimated total investment of approximately USD 200 million with operations scheduled to begin by the end of 2026 With a total investment of approximately USD 200 million the project is expected to commence construction in 2024 and commence operations by the end of 2026 The project will address Indonesia's urgent waste management challenges while also significantly reducing greenhouse gas emissions it will create numerous job opportunities for the local community and drive the development of related industries in Indonesia reinforcing the country's commitment to carbon neutrality goals and sustainable development efforts Sign InJoinLink Live Streaming Semen Padang Vs PSM Makassar di BRI Liga 1 | OneFootballBola.com Bola.com, Jakarta - Link live streaming Semen Padang melawan PSM Makassar pada lanjutan BRI Liga 1 2024/2025 Semen Padang akan menghadapi tantangan berat pada lanjutan BRI Liga 1 2024/2025 Armada Eduardo Almeida itu dijadwalkan berjumpa tim papan atas PSM Makassar dalam laga pekan ke-11 Tim berjulukan Kabau Sirah itu kini berada di dasar klasemen atau peringkat ke-18 BRI Liga 1 dengan torehan lima poin dari 10 pertandingan. Adapun PSM Makassar bertengger di urutan keenam dengan koleksi 17 angka Laga Semen Padang versus PSM Makassar diprediksi berjalan menarik dan ketat Terlebih bagi tuan rumah yang saat ini berusaha keras keluar dari zona degradasi Tambahan poin sangat penting buat memperbaiki posisi di papan klasemen Essential digital access to quality FT journalism on any device Complete digital access to quality FT journalism with expert analysis from industry leaders Complete digital access to quality analysis and expert insights complemented with our award-winning Weekend Print edition Terms & Conditions apply Discover all the plans currently available in your country Digital access for organisations. Includes exclusive features and content. See why over a million readers pay to read the Financial Times. 01 Feb 2025 12:00:00 GMT?.css-1txiau5-AnswerContainer{color:var(--GlobalColorScheme-Text-secondaryText2);}Persib Bandung won 1–0 over PSM Makassar on Sat The current head to head record for the teams are Persib Bandung 4 win(s) Have scored 8 goals in their last 5 matches PSM Makassar haven't lost to Persib Bandung in their last 5 meetings (3W, 2D). 01 Feb 2025 12:00:00 GMT?Persib Bandung won 1–0 over PSM Makassar on Sat 01 Feb 2025 12:00:00 GMT.InsightsHave scored 6 goals in their last 5 matches PSM Makassar haven't lost to Persib Bandung in their last 5 meetings (3W Persib Bandung is playing home against PSM Makassar at Gelora Bandung Lautan Api Stadium on Sat ZTE Chairman Fang Rong: Technology for Good Co-creating a Green BRICS Sustainable Future ZTE earns GlobalData's highest rating for IP Core and Edge Routers reinforcing Telkomsel's  end-to-end AI and ML driven approach (Hyper AI) aimed at delivering customer-centric network excellence Indonesia, 21 November 2024 – Telkomsel Indonesia's leading digital telecommunications provider in collaboration with ZTE Corporation (0763.HK/000063.SZ) a global leading provider of integrated information and communication technology solutions has introduced an AI and Machine Learning (ML) based network solution to enhance the quality of its 4G/LTE services in Makassar and Kendari Known as the Self-Adaptive Feedback solution this advanced technology marks the continuation of the two companies' collaboration at Mobile World Congress 2024 in Barcelona As the demand for high-speed internet continues to grow Telkomsel and ZTE have introduced the Self-Adaptive Feedback solution to optimize network performance without requiring additional hardware This technology enables service providers to automatically adjust network parameters resource-demanding applications such as video streaming and gaming the solution helps reduce operational costs through energy efficiency Telkomsel's Chief Strategic Planning Officer, stated "In line with our commitment to continuously improving service quality through the latest technological innovations Telkomsel and ZTE have reviewed and implemented the Self-Adaptive Feedback solution in Makassar and Kendari to provide a smoother and more efficient internet experience We hope that the adoption of cutting-edge technology like Self-Adaptive Feedback – as part of Telkomsel's Hyper AI approach – will not only enhance customer satisfaction but also strengthen our position as an industry leader consistently delivering positive impacts and benefits for Indonesia." Network tests with Self-Adaptive Feedback in Makassar and Kendari have shown a significant improvement in user experience Video buffering has decreased by 15% while download speed has increased by 11% and webpage loading times have improved by nearly 30% Gaming latency has also decreased by up to 47% offering a more responsive gaming experience Self-Adaptive Feedback technology is equipped with energy-saving features that automatically shift base stations to low-power mode during low-traffic periods Power efficiency has improved by around 15% while energy consumption has decreased by 8% President Director of ZTE Indonesia, stated "We are proud to collaborate with Telkomsel in delivering innovative solutions that can support user needs we believe that every innovation brings new hope and opens the door to various opportunities in the future By integrating artificial intelligence into the network we not only enhance efficiency but also address the increasing demand for access to high-quality digital content we are committed to improving the quality of digital access in Indonesia and making a positive contribution to society." Self-Adaptive Feedback technology has been deployed at over 90 Telkomsel sites As part of the Hyper AI approach – particularly within Telkomsel's  leading Autonomous Network architecture that leverages AI for faster and more reliable network service automation – the implementation of Native AI will be expanded across the entire Telkomsel-ZTE network in Indonesia This initiative aims to accelerate digital progress across various sectors Although Thai League 1 outfits Buriram United and BG Pathum United were widely regarded as the frontrunners CAHN rose to the occasion right from the start and swept aside all who stood in their way Starting with a statement 2-1 win over Buriram, CAHN racked up four more consecutive victories over Lion City Sailors, Kaya FC-Iloilo, Kuala Lumpur City and Borneo Samarinda to be the only team with a perfect record in the group stage Facing off against Group A runners-up PSM Makassar in the semis CAHN were widely expected to continue their winning ways -- and for good reason After all, this has been a side littered with Vietnam internationals -- many of whom were involved in the recent ASEAN Championship triumph at the start of the year *Catch all the ASEAN Club Championship action live on the ASEAN United FC YouTube channel here CAHN's 15 goals also made them the most-prolific outfit in the group stage while the defence also stood up well as they conceded just five at the end of 90 minutes at the start of the month CAHN found themselves on the receiving end of a 1-0 first-leg defeat Credit has to go to PSM as they remained resolute despite having just 37% of the possession while also boasting just six shots to CAHN's 11 With one of just two efforts on target, PSM snatched the victory in the 80th minute as Yuran Fernandes met a corner with a perfectly-guided header into the back of the net. View this post on Instagram A post shared by ASEAN United FC (@aseanutdfc) the Liga 1 outfit will be aware that they can ill afford to get ahead of themselves CAHN have notched three wins and a draw on the domestic front Coached by Alexandré Pölking, who famously presided over Thailand's most-recent era of success CAHN will be hoping they are now firmly back on the right track following a slump in form which saw them pick up just one win in seven outings Currently 6th in V.League 1, a repeat of their stunning 2023 title triumph -- which came in their first season after promotion -- looks beyond them although the ten-point gap between them and leaders Thép Xanh Nam Định is still mathematically assailable with a game in hand Success could also come in the form of the Vietnamese Cup with a semifinal against Thể Công-Viettel taking place next month But perhaps CAHN's best chance of silverware this season remains in the one competition where they have looked the team to beat all campaign long they need to overturn a deficit on Wednesday evening and book their place in the ASEAN Club Championship final Cari English Pampering Your Tongue at Makassar Street Culinary TEKS › English›Pampering Your Tongue at.. Iklan Pampering Your Tongue at Makassar Street Culinary The presence of street culinary in Makassar not only drives the economy Audio Berita This article has been translated using AI. See Original TEKS The following article was translated using both Microsoft Azure Open AI and Google Translation AI. The original article can be found in Memanjakan Lidah di Kuliner Jalanan Makassar The atmosphere of the street food area of Pasar Cidu in Makassar was buzzing with visitors on Saturday (30/9/2023) Born from the initiative of residents trying to recover from the pandemic this culinary center has transformed quiet and crime-prone places in corners of the city into bustling with residents' economic activity It doesn't take a lot of money to enjoy delicious food on the streets of Makassar City One of the culinary centers that many residents currently visit is Pasar Cidu street food on Jalan Tinumbu both on the left and right sides of the road Japanese food in the form of soup containing meat which is an oriental snack made from chicken or beef residents can also taste fried shrimp with flour traders on the streets of Pasar Cidu also sell various cakes Gogos is a traditional Makassar cake made from sticky rice filled with meat wrapped in leaves and baked It is already possible to enjoy several types of food and drinks At least there is an alternative place to hang out and eat with friends," said M Yusuf (19) a Psychology student at Makassar State University who visited the place on Saturday (30/9/2023)." culinary carts were lined with bright lights Cidu Market looks like a street food area in Singapore or Bangkok Also read: Makassar, Fish Culinary Heaven A seller serves customers in the culinary area of Pasar Cidu street market The street food culture in this area is quite bustling sit on a mat laid along the sidewalk or the front of a shop behind the row of carts The hustle and bustle is far from the image of the Pasar Cidu area a few years ago It was even often used as a place to drink and party for groups of young people The Cidu Market is estimated to have existed since the 1950s one of the oldest markets in Makassar city only consisted of fish and vegetable traders who sold their wares using mats or carrying poles The area on Jalan Tinumbu was later named Cidu Market because it was located on a triangular-shaped land Cidu in the Makassar language means sharp or pointed Of all the street culinary delights in Makassar Pasar Cidu street food is indeed the busiest This also inspired the birth of other culinary markets One of the community leaders in Cidu Market stated that the market has been on fire several times and the vendors were relocated to a new location currently known as Pannampu Market Some sell their goods in alleys and front yards of houses The former Cidu Market was then turned into a terminal and now it's full of buildings and gas stations," he said The presence of street food vendors is like reviving the old Cidu Market but with different appearances and offerings which is also known as the North because of its location in the north of Makassar Also read: Culinary Hunting on the South Sulawesi Route It was often used as a place for young people to drink and get drunk What's more important is that there is money circulating and residents are more well-off," said Ridwan As a resident living around the Cidu Market since childhood They organize parking and help maintain security Buyers are queuing up at one of the food carts in the street food area of Pasar Cidu This area is one of the main street food centers in Makassar The hustle and bustle of the Pasar Cidu area has been particularly noticeable in the past two years The presence of street food vendors at Pasar Cidu has inspired similar activities on the streets of Makassar The city's residents are also benefitting from this night-time culinary activity The portion is more than enough for a side dish accompanied by a plate of rice and he sells until 01.00 WITA in the morning sometimes the merchandise is sold out in just a matter of hours have sweetness and are krenyes-krenyes," he said who sells mixed fruit salad and spicy fruit salad He has a wide selection of fruits to choose from Also read: Going to Makassar? Come on, “Makang Ikang” a 30-year-old vendor in the street food arena of Pasar Cidu in Makassar This area has become a hub for affordable culinary options "Mixing it up or choosing only the fruits you like is possible That's why I never miss buying asinan when I come here It still keeps well when stored in the fridge," said Hasriani (20) Hasriani is one of the regular visitors to the Cidu Market "Fifty thousand rupiah for a café visit is not enough I can be full and even take some home," she said street food is now popular in various areas in Makassar one of the oldest night markets in Makassar culinary delights are now also sought after Initially the market on Jalan Cendrawasih was only filled with clothing and shoe traders traders come to put up tents and arrange sales causing traffic jams in the surrounding area Visitors who finish exercising walked among the tents selling clothes and cuisine in the GOR Sudiang area Several culinary markets have started to emerge in Makassar during and towards the end of the pandemic but have become increasingly crowded in recent years these culinary hubs are born out of citizen initiatives Some citizens originally started trading food but there are also those who only started trading when street food became popular street food vendors are also changing part of the face of the GOR Sudiang area it was abandoned and surrounded by overgrown vegetation it is now becoming cleaner and more crowded more and more Sudiang residents are utilizing this area for sports activities or various events Every morning until afternoon on weekends and holidays food vendors will fill the area around the GOR There are also clothing vendors who sell their wares every day The hustle and bustle of the GOR Sudiang area will be visible when residents who have finished exercising join in eating and drinking at the food stalls Visitors crowded in front of the food carts in the street food area of Pasar Cidu I sell again until the evening," said Musrifah (42) precisely on the edge of Tunggu Pampang Reservoir cafes are also lined up and equally crowded There is also a street food area at Pasar Segar and even in the elite Center Point of Indonesia area located in the middle of Makassar City All of them serve delicious and relatively affordable food See also: Creativity of Processed Bananas from Makassar The existence of street food in various corners of the city has a significant impact not only on the economy but also on job creation and the development of small and medium enterprises Street food also transforms the surrounding areas into a more friendly and welcoming place for everyone especially for those who want to indulge in a feast until they are satisfied The rise of street food in Makassar is also in line with the branding or imagery promoted by the Makassar City Government This Branding was launched by the Mayor of Makassar It is hoped that this Branding will not just be a slogan but will further strengthen Makassar's emergence as a city where good food can be eaten We use cookies to distinguish you from other users and to provide you with a better experience on our websites. 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BrowseServicesOpen researchInstitution LoginSearchMenu links This article describes the evolution of the commercial connections between China and the southern Sulawesian port of Makassar from the beginning of the seventeenth century until 1669 when the Dutch Vereenigde Oost Indische Compagnie conquered Makassar It attempts to show that these connections went through several transformations Initially direct Chinese shipping supplied Makassar with Chinese goods but this direct trade lasted only about a decade commerce carried by Macanese ships and trade in Indochinese ports that were frequented by both Sulawesian and Chinese vessels maintained the commercial connection This connection in its different forms allowed Makassar to act as an entrepôt that supplied Chinese goods and Japanese copper to more distant parts of Southeast Asia especially those in the eastern Indonesian archipelago The article concludes by arguing that after the conquest of Makassar Banjarmasin in southern Borneo developed as a new regional entrepôt connecting China to the eastern archipelago This article traces the connections between the trading network centred on the port city of Makassar in the southern Sulawesian kingdom of Gowa and the trading network of China's maritime merchants during the mid-seventeenth century It also attempts to periodise the relationship between these two trading systems from the establishment of their first connection in or around the year 1613 until the conquest of Gowa by the Dutch Vereenigde Oost Indische Compagnie (VOC) in 1669 The most basic argument that the article makes is that although the connections between the Chinese and Makassar networks went through a series of transformations they allowed Makassar to act as a distribution hub for Chinese products Sulawesian merchants carried these products to the parts of the eastern archipelago that the VOC had isolated from other trading networks as well as a number of more western and southern ports that they visited The present article will take a different approach It will focus on the movement of trade goods rather than ships or people and will attempt to show that in the seventeenth century the reach of overseas Chinese trading networks extended further than Chinese ships actually sailed thanks to links between them and the trading systems of other groups in Southeast Asia Makassar was able to play an important role in the redistribution of Chinese goods long before the VOC's conquest of Gowa despite the absence of direct Chinese shipping Several indirect connections to Makassar brought Chinese porcelain and a variety of other goods exported or re-exported from Chinese ports and these were in turn sent to markets in the eastern part of the Indonesian archipelago or to Makassar's important trading partners in the west This indirect flow of goods from the cargo holds of Chinese ships through intermediary ports to Makassar's harbour and then on to more distant markets is evident in our sources but has largely fallen through the gap between the scholarship focussed on Makassar's pre-1669 trading world and the scholarship focussed on the trials and tribulations of the Zheng family This article is an attempt to bridge this gap The one type of scholarship that has acknowledged the commercial connection between Makassar and China in the mid-seventeenth century has focussed on the activities of Portuguese traders sailing from Macau, particularly the work of George Bryan Souza and Roderich Ptak.Footnote 7 This article's central concern is the flow of Chinese trade goods which were originally exported or re-exported from China by Chinese ships but because the trade between Macau and Makassar carried by Portuguese merchants also supplied Chinese goods the analysis here will necessarily draw upon the works of Souza and Ptak to analyse the relative importance of the different connections between China and Makassar It will argue that although the Macanese connection may have sometimes been the most important link especially in the last decade or so of Gowa's independence the inclusion of Makassar on the Selden Map along the northern littoral route of the Java Sea does tally with other sources because if Chen Jiarong is correct in dating the map to 1624 Chinese merchant vessels would have been visiting the city for more than a decade by then according to the 1613 English report this move by the Dutch company also hints that there were Chinese ships arriving at least sporadically in Makassar up until that year which would have justified the port's inclusion in the Selden Map it was likely a result of changes taking place within the structures of the trading systems of both China's coast and the Indonesian archipelago it became focussed on the incredibly lucrative silk-silver trade in Japan and Luzon at the expense of trade in other Southeast Asian ports the Portuguese were carrying archipelagic goods back to Macau and these were distributed through the Chinese coastal trading network creating more competition in what seems to have been an increasingly limited market Zheng Chenggong 鄭成功 (still often referred to as “Koxinga” in European language scholarship) Zheng Zhilong's son and successor as patriarch of the family from 1646 to 1662 had managed to take control of most of China's foreign trade Acting both as a merchant prince and a Ming loyalist warlord he slowed Qing advances into south-eastern China and supported his military forces with more direct control over the merchant fleets that had previously been relatively loosely affiliated with his father He accomplished this with an impressive naval force and unintentional help from the Manchu Qing's anti-trade policies in the parts of China that the new empire controlled Despite Zheng Chenggong's relatively greater interest in Southeast Asia, Makassar was still not part of the family's business strategy. Although on at least one occasion the family's merchants did send a ship to Manado on the Minahasa peninsula of northern Sulawesi for rice,Footnote 42 there is no record of any Chinese trading vessels sailing from China to Makassar during Zheng Chenggong's rule The Zheng organisation's continued lack of interest in the port was likely because the already limited market for eastern archipelagic goods in China declined even further thanks to economic disruption and poverty caused by the Ming-Qing war Whatever demand remained for these types of goods was probably satisfied either by supplies from the VOC's factory in Taiwan or by Zheng trade in the Indochinese ports which were still linked to Sulawesi and the eastern archipelago by Makassar's own trading fleet just as the clause requiring the exclusion of Chinese trade was but they suggest that the Dutch as well as the English recognised that trade with Cambodia was an important trading partner for Makassar which was the primary destination for most high value-per-mass Chinese goods in Manila so they likely represented a “spill over” from the Zheng family's trade to Luzon the reversal of this commercial flow does testify to the relative weakness of the Macanese trade in Makassar after 1660 and to the gathering strength of the Zheng family's trade in Manila after 1663 when Zheng Jing ended the family's hostile stance towards the Spanish colony the greatest beneficiary of the company's rule in Makassar This article has sought to trace the development of Makassar's relationship with Chinese trading networks from the port city's rise as a major emporium around the turn of the seventeenth century until its occupation by the Dutch VOC in 1669 there was no direct trade between China and Makassar other than that carried by Portuguese ships from Macau and consequently past scholarship that has dealt with the relationship between China and Makassar has focussed almost exclusively on the period after 1669 or on the activities of Macau-based Portuguese merchants even Cheng Wei-chung's recent detailed study of the Zheng family's trading system based on the VOC's archives has not examined the influence of the family's trade beyond the regions in Southeast Asia that their ships actually sailed to The analysis presented here shows that despite the lack of a direct connection the Zheng family's trading system and Makassar were connected by a flow of Chinese goods through intermediary ports These indirect connections allowed Makassar to function as a major link between Chinese trade in the western part of Southeast Asia and the eastern archipelago and some of these certainly found their way back to China some and perhaps most of the Chinese goods that arrived at Makassar were sent eastwards to satisfy the consumer markets of the eastern archipelago The article also attempts to connect the development of these trading links between China and the eastern archipelago during the mid-seventeenth century with those that developed after 1669 Drawing on the work of Heather Sutherland and others its conclusion is that Makassar ceased to function as a major commercial hub between the post-1683 Qing Chinese trade network and the archipelago demand for Chinese goods in the archipelago did not diminish and Chinese demand for tropical goods probably grew as China recovered from its long period of war and upheaval in the last two decades of the seventeenth century which had been an important pepper supplier to Makassar and indirectly much of the rest of Asia throughout the middle of the seventeenth century emerged as a new emporium supplying both pepper and eastern archipelagic goods to Chinese merchants who began sailing there directly in the late 1680s or early 1690s Makassar gradually developed a new role in China's expanding eighteenth-century trading system by becoming an entrepôt for turtle shell and trepang I would like to thank Lin Man-houng and Pin-Tsun Chang for their comments on the original version of this paper as well as the Maritime History Studies Program of the Research Center for Humanities and Social Sciences at Academia Sinica for hosting the workshop where I originally presented it who was incredibly helpful and encouraging during the revision process and to the two anonymous reviewers for their keen insights Part of the research for this article was made possible by support from the National Science and Technology Council of Taiwan (grant number 112-2410-H-004-005-MY3) “Scramble for Spices: Makassar's Role in European and Asian Competition in the Eastern Archipelago Up to 1616,” in The Dutch and English East India Companies: Diplomacy Adam Clulow and Tristan Mostert (Amsterdam: Amsterdam University Press “Ubiquitous but Elusive: The Chinese of Makassar in VOC Times,” Journal of Asian History 47 “Taiwan Ming-Zheng yu dong nan ya zhi mao yi guan xi chu tan: Fa zhan dong nan ya mao yi zhi dong ji shi wu ji wai shang zhi qian lai 臺灣明鄭與東南亞之貿易關係初探: 發展東南亞貿易之動機、實務及外商之前來” (A preliminary study of the trading relationship between the Ming-Zheng of Taiwan and Southeast Asia: Motivations and foreign merchants in the development of trade in Southeast Asia) Taiwan shi fan da xue li shi xue bao 臺灣師範大學歷史學報 14 (1986) City and Systems,” in Environment Trade and Society in Southeast Asia: A Longue Durée Perspective David Henley and Henk Schulte Nordholt (Leiden: Brill 1613–1667,” Journal of the Malayan Branch of the Royal Asiatic Society 31:1 (May 1958) Bassett notes that one English company employee had arrived in October 1612 on a Guajarati ship from Patani so we can tentatively assume no Chinese vessels from China arrived during the winter monsoon of 1612/1613 “Chinese Maritime Trade: The Case of Sixteenth-Century Fu-chien (Fukien)” (PhD diss. Liang zhong hai dao zhen jing 兩種海道針經 (Two maritime rutters) (Beijing: Zhong hua shu ju Mills believes that one route described in the Shun feng xiang song terminates somewhere in northern Sulawesi although the rutter's text makes this quite uncertain Dong xi yang kao 東西洋考 (Record of the eastern and western oceans) (Beijing: Zhong hua shu ju 17 For the dating of the map see Chen Jiarong 陳佳榮 “‘Ming wei jiang li ji Zhang Quan hang hai tong jiao tu’: Bian hui shi jian te se ji hai wai jiao tong di min glue xi 《明末疆里及漳泉航海通交圖》編繪時間 特色及海外交通地名略析” (Notes on the Selden Map of China with a focus on its compilation “The Origins of the Selden Map of China: Scientific Analysis of the Painting Materials and Techniques Using a Holistic Approach,” Heritage science 4:28 (2016) 19 Chen Jiarong 陳佳榮, “Shun feng xiang song zuo zhe ji wan cheng nian dai xin kao 《順風相送》作者及完成年代新考” (Notes on the Shun-feng Xiang-song (Fair winds for escort) with a focus on its author and compilation) in “Nan ming wang 南溟網,” http://www.world10k.com/blog/?p=2028 De Jacht op sandelhout: De VOC en de tweedeling van Timor in de zeventiende eeuw (The hunt for sandalwood: The VOC and the division of Timor in the seventeenth century) (Zutphen: Walburg Pers The Italian was named Giovanni Maria Moretti and his relationship with the VOC has very recently been examined by Tristan Mostert the Dutch East India Company and the struggle for the Ambon Islands (c 23 The partial exception is a mention of a Chinese merchant and resident of Makassar who the English company servant claims was bound on a trading voyage in 1655 intending to sail first to Batavia and then on to China 25 The best overview of Zheng Zhilong's rise and the strategies of the Anhai merchants is Cheng For an overview of the Ming dynasty's regulation of maritime trade “Chinese Maritime Trade.” For an older but still very useful overview of maritime Chinese history from the mid sixteenth century to late seventeenth “Maritime China from Wang Chih to Shih Lang: Themes in Peripheral History,” in From Ming to Ch'ing: Conquest and Continuity in Seventeenth-Century China Les Philippines et le Pacifique des Ibériques (XVI e XVIII e siécles) Introduction Méthodologique et Indices d'activité (Paris: S.E.V.P.E.N. “The Door Ajar: Japan's Foreign Trade in the Seventeenth Century” (PhD diss. 31 General Missive by Governor-General and Council in Generale missiven van gouverneurs-generaal en raden aan Heren XVII der Verenigde Oostindische Compagnie (General missives of governors-general and councils to the Heren XVII of the Dutch East India Company) 32 General Missive by Governor-General and Council 34 De Dagregisters van het Kasteel Zeelandia 1629 – 1662 (Daily registers of Fort Zeelandia For an overview of the complicated relationship between the VOC and Zheng Zhilong's organisation “The Company's Chinese Pirates: How the Dutch East India Company Tried to Lead a Coalition of Pirates to War against China Journal of World History 15:4 (2005): 415-44 Bouwstoffen voor de geschiedenis der Nederlanders in den Maleischen Archipel (Materials for the history of the Dutch in the Malay Archipelago) ('s-Gravenhage: Martinus Nijhoff 36 General Missive by Governor-General and Council 38 For an example of the Chinese exports brought by Macanese ships Dagh-register gehouden int Casteel Batavia vant passerende daer ter plaetse als over geheel Nederlandts-India: Anno 1636 (Daily register kept in Batavia Castle concerning what passed there as well as throughout the Dutch Indies: Year 1636) ('s-Gravenhage: Martinus Nijhoff “Taiwan Ming-Zheng yu dong nan ya zhi mao yi”: 71 42 General Missive by Governor-General and Council “English Trade in Celebes,” 29–30; and Leonard Y The Heritage of Arung Palakka: A History of South Sulawesi (Celebes) in the Seventeenth Century (The Hague: Martinus Nijhoff Embassies and Illusions: Dutch and Portuguese Envoys to K'ang-his 1666 – 1687 (Cambridge: Harvard University Press 50 Anthony Farrington and Dhiravat na Pombejra Another indirect piece of evidence for the importance of Makassar's commercial relationship with Siam is the large number of Makassarese refugees who settled in Ayutthaya after VOC's conquest of their homeland in 1669 “A Political History of Siam under the Prasatthong Dynasty Murder and Mayhem in Seventeenth-Century Cambodia: Anthony van Diemen vs King Ramadhipati I (Chiang Mai: Silkworm Books De Oost-Indische Compagnie in Cambodja en Laos: Verzameling van bescheiden van 1636 tot 1670 (The East India Company in Cambodia and Laos: Collection of records from 1636 to 1670) ('s-Gravenhage: Martinus Nijhoff “Reconceptualizing Southern Vietnamese History from the 15th to 18th Centuries: Competition along the Coasts from Guangdong to Cambodia” (PhD diss. “De handelsrelaties van het Makassaarse rijk volgens de Notitie van Cornelis Speelman uit 1670,” (The trade relations of the Makassarese Empire According to the Notice of Cornelis Speelman in 1670) Nederlands Historische Bronnen 3 (1983) 63 Ibid. 65 See for example the goods listed in Paulus de Brievings and Jacob Cloeck The Maguindanao Sultanate in the 17th Century: Triumph of Moro Diplomacy (Quezon City: New Day Publishers 207; and the types of sundry goods carried aboard a Chinese vessel shipwrecked off the coast of modern Vietnam in 1690 that are listed in Michael Flecker Vietnam,” International Journal of Nautical Archaeology 21:3 (1992) “Tardíos amores insulindios: Manila y el sultanato de Macasar en el siglo XVII,” (Insulindian late loves: Manila and the sultanate of Makassar in the 17th century) Vegueta: Anuario de la Facultad de Geografía e Historia 20 (2020) 68 Ibid. 70 For the development of the Zheng family's trading system in the 1660s 76 General Missive by Governor-General and Council General Missive by Governor-General and Council A Voyage to and From the Island of Borneo in the East Indies (London: T Too many automated requests from this network - No HTML tags allowed- Web page URLs will display as text only- Lines and paragraphs break automatically- Attachments Your email address will be used in order to notify you when your comment has been reviewed by the moderator and in case the author(s) of the article or the moderator need to contact you directly shared ownership in or any close relationship with any organisation whose interests may be affected by the publication of the response Please also list any non-financial associations or interests (personal religious or other) that a reasonable reader would want to know about in relation to the submitted work This pertains to all the authors of the piece Metrics details The extreme conditions of the deep-sea environment create a natural habitat for deep-sea bacteria These remarkable microorganisms have developed unique strategies to survive and adapt to their surroundings research on the diversity of deep-sea bacteria both culture-dependent and culture-independent This study focused on exploring the biodiversity of deep-sea bacteria specifically in the Makassar and Lombok Strait the main Indonesian throughflow pathway characterized by relatively fertile water which serves as an important deep-sea region High-throughput DNA sequencing of full-length 16S rRNA was employed to construct a genomic database The results of the bioinformatic analysis revealed that two stations exhibited a more similar community structure of deep-sea bacteria than did station 33 (Lombok Strait) Among the predominant phyla found at a depth of 1000 m and Psychrobacter consistently appeared at all three stations albeit with varying relative abundance values These bacterial genera share common characteristics and are commonly found in deep-sea ecosystem The environmental conditions at a depth of 1000 m were relatively stable phosphate 6.32 µmol/L and dissolved oxygen (DO) 2.90 mg/L The bacterial community structures at the three sampling stations located at the same depth (1000 m) exhibited similarities as indicated by the closely aligned similarity index values The physical characteristics of the deep sea differ from those of shallow waters The deep sea is characterized by high pressure Bacteria might also have defenses against cold temperatures that harm cell structures These geographical conditions create an exceptional and diverse habitat that sustains a wide array of marine life The exploration of metagenomics in the study of deep-sea bacteria within the Makassar Strait is particularly intriguing there is a notable absence of data pertaining to this specific location the primary objective of this research was to determine the richness and variety of deep-sea bacteria by leveraging the 16S ribosomal RNA approach This undertaking serves as an inaugural step in characterizing the deep-sea bacterial diversity within Indonesian waters paving the way for subsequent bioprospecting initiatives based on genomic information The hope is that this research will make a valuable contribution to our broader understanding of marine microbiology while shedding light on the pivotal role of marine bacteria in maintaining equilibrium within deep-sea ecosystems (a) The sampling location within the Makassar Strait represents a pivotal route for water movement from the tropical Pacific Ocean to the Indian Ocean The microorganism station is marked by the red circle (b) The bathymetric transect from station 50 and 48 (Makassar Strait) to station 33 (Lombok Strait) Deep-sea water samples were collected from a 1000 m depth using a Carousel Water Sampler type SBE 32 equipped with a conductivity temperature depth (CTD) type SBE 911 + (Sea-Bird Scientific) to measure environmental parameters of seawater (pressure Phosphate and nitrate concentrations were measured using a spectrophotometer (UV–Vis Shimadzu 1700 CTD casts were deployed from the surface to a depth of 1000 m The recording interval was set to 32 measurements/second and the casting speed was kept at a maximum of 50 m/min to minimize noise during the measurements 5 L of seawater was filtered through 0.22 μm pore-size cellulose nitrate membrane filters with diameter of 47 mm The filters were stored in falcon tubes at − 20 °C onboard and in the laboratory DNA concentration and purity were assessed using 1% agarose gels the DNA was subsequently diluted to 1 ng/μL using sterile water The same volume of 1 × loading buffer (SYBR Green) was mixed with the PCR products which were then subjected to electrophoresis on a 2% agarose gel for detection The PCR products were combined in equal density ratios and purified using a Qiagen Gel Extraction Kit (Qiagen Based on environmental parameter data measured at a depth of 1000 m The alpha diversity index (Table 1) serves as a metric for characterizing the average species diversity within a specific location or habitat on a local scale This index quantifies the number of distinct species present within a given community or ecosystem at a particular location The highest diversity index was found in the Makassar Strait followed by Station 33 in the Lombok Strait in conjunction with the Simpson index approaching 1 collectively indicates a well-balanced and diverse community structure characterized by a high degree of species richness and an equitable distribution of individuals among species (a) Relative abundance of deep-sea bacteria at the Phylum between two location Makassar Strait (sta 48 & sta 50) and Lombok Strait (sta 33) (b)Ternary plot station 33, 48 and 50. UPGMA-clustered dendrograms based on Bray–Curtis dissimilarity and Relative abundance of deep-sea bacteria at the Genus between three different station Sankey diagram visualization of bacterial diversity at the species level (a) station 33 (a) Temperature and Salinity (TS) diagram and (b) Vertical profile of Dissolve Oxygen the summary is that the water mass at a depth of 1000 m in the Makassar Strait is not connected with the water mass at the same depth in the Lombok Strait and is subject to significantly different oceanographic dynamics and Psychrobacter across all stations suggests these deep-sea bacterial genera are well-adapted to high salinity This study provides a significant contribution to our understanding of deep-sea bacterial biodiversity within the 1000 m water column along the Indonesian Throughflow (ITF) route specifically in the Makassar and Lombok Straits The findings reveal a high level of biodiversity reflecting the complexity of the deep-sea ecosystem in these regions This ecosystem is characterized by extreme environmental conditions which collectively create a habitat for a highly specialized and exclusive bacterial community this study is the first to document the presence of Colwellia psychrerythraea and Psychrobacter pacificensis in the deep-sea waters of Indonesia specifically within the 1000-m water column future research should explore the ecological roles and metabolic pathways of these microorganisms as well as their interactions within the tropical deep-sea ecosystem bioprospecting could uncover novel enzymes or metabolites with applications in biotechnology According to the analysis of the metagenomic data the bacterial community profiles at the three stations exhibited relatively similar bacterial types The primary phyla most prevalent at this 1000-m depth across all three stations were Proteobacteria these bacterial genera share common characteristics and are commonly found in deep-sea environments The physicochemical conditions of the water at these three locations a more comprehensive study is necessary to discern the influence of these environmental conditions on the distribution of bacterial communities and other factors that have yet to be investigated The raw read sequences have been deposited in GenBank (NCBI) under the Accession Number PRJNA1046651 New perspectives in benthic deep-sea microbial ecology Coupled RNA-SIP and metatranscriptomics of active chemolithoautotrophic communities at a deep-sea hydrothermal vent Microorganisms from deep-sea hydrothermal vents Bacterial and fungal diversity in sediment and water column from the abyssal regions of the Indian Ocean Phylogenetic identification of marine bacteria isolated from deep-sea sediments of the eastern South Atlantic Ocean Metagenomic analysis and metabolite profiling of deep-sea sediments from the Gulf of Mexico following the Deepwater Horizon oil spill a novel piezophilic species from deep-sea sediments of the Japan Trench and sustainability of the global deep-sea sponge microbiome Compositional and quantitative insights into bacterial and archaeal communities of south pacific deep-sea sponges (Demospongiae and Hexactinellida) Microbial oceanography of the dark ocean’s pelagic realm Properties and applications of extremozymes from deep-sea extremophilic microorganisms (2019) Metagenomic analysis of bacterial communities of antarctic surface snow Metagenomics approaches in discovery and development of new bioactive compounds from marine actinomycetes Taxonomic and functional profiling of the microbial communities of Arabian Sea: A metagenomics approach Bioprospection of marine microorganisms: Biotechnological applications and methods | Bioprospección de microorganismos marinos: Aplicaciones biotecnológicas y métodos Marine metagenomics as a source for bioprospecting Metagenomics-based drug discovery and marine microbial diversity Newly designed 16S rRNA metabarcoding primers amplify diverse and novel archaeal taxa from the environment Variability of Indonesian throughflow within Makassar Strait Oceanography surrounding Krakatau Volcano in the Sunda Strait Seasonal and interannual variability of tidal mixing signatures in Indonesian seas from high-resolution sea surface temperature (2022) Vertical profile of culturable bacteria from the Makassar Strait Performance of neural network basecalling tools for Oxford Nanopore sequencing NanoPack: Visualizing and processing long-read sequencing data Centrifuge: Rapid and sensitive classification of metagenomic sequences On some properties of the Bray–Curtis dissimilarity and their ecological meaning BioSankey: Visualization of microbial communities over time Software Manual Seasoft V2 : SBE Data Processing Vennpainter: A tool for the comparison and identification of candidate genes based on Venn diagrams Gordon, A. 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A novel Halomonas species isolated from a deep-sea sediment sample of the new Britain trench exhibits high anti-oxidative stress capability. 2925–2925 (2020). https://doi.org/10.46427/gold2020.2925 L-glutaminase synthesis by marine Halomonas meridiana isolated from the red sea and its efficiency against colorectal cancer cell lines Microbe profile: Alteromonas macleodii—A widespread Phylogenetic analysis of psychrophilic bacteria isolated from the Japan Trench including a description of the deep-sea species Psychrobacter pacificensis sp Download references Our sincere gratitude goes to the captain and the dedicated crew of R/V Baruna Jaya VIII during the 2019 TRIUMPH expedition for their invaluable assistance in the sampling process We extend our appreciation to the research teams from the First Institute of Oceanography (FIO) which allowed us to benefit from their wealth of knowledge and experience throughout the TRIUMPH Cruise This study also received partial funding through the Excellence in Research Program under the Institut Teknologi Bandung—Research Center Scheme for 2023 and supported by the Rumah Program “Pengungkapan dan Pemanfaatan Biodiversitas Nusantara" 2023 of the Research Organization for Life Sciences and Environment This work is also supported by the Physical Oceanography Program of the U.S National Aeronautics and Space Administration (NASA; Grant #80NSSC18K0777) and National Sciences Foundation (NSF; Grant 2242151) through the University of Maryland for R.D.S Rafidha Dh Ahmad Opier & Ocky Karna Radjasa Department of Atmospheric and Oceanic Science and Key Laboratory of Marine Science and Numerical Modeling Center of Collaborative Research on Aquatic Ecosystem in Eastern Indonesia play a role in the process of sampling and measuring environmental data on board All authors have read and agreed to the final version of the manuscript The authors declare no competing interests Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Below is the link to the electronic supplementary material Download citation DOI: https://doi.org/10.1038/s41598-024-74118-9 Sign up for the Nature Briefing: Microbiology newsletter — what matters in microbiology research