Mantan Pemain Sirkus OCI Desak SP3 Dicabut Calon Jemaah Haji Majene Hiasi Koper dengan Tanda Unik Update Pencarian Korban KMP Muchlisa: Basarnas Terjunkan Penyelam Lippo Cikarang Bukukan Pra-penjualan Rp 323 Miliar pada Kuartal I 2025 Pertumbuhan Ekonomi 4,87 Persen Tetap Positif di Tengah Krisis BEI Terbitkan Edukasi Baru Waran Terstruktur Konklaf 2025 Diprediksi Hanya Berlangsung 2-3 Hari 133 Kardinal Sudah Berada di Vatikan untuk Konklaf 2025 Gedung Putih Keliru Tampilkan Meme Trump Jadi Tokoh Jahat di Star Wars Prediksi Inter vs Barcelona: Flick Mungkin Mainkan Robert Lewandowski Indro Sebut Pengurus Jadi Politisi Langgar AD/ART Jonathan Frizzy Terancam 12 Tahun Penjara karena Kasus Vape Ilegal Popemobile Paus Fransiskus Jadi Klinik di Gaza Apa Itu World App yang Viral karena Imbalan Rp 800.000 Pendaki Jatuh Dalam Jurang Gunung Saeng Bondowoso Pendaki Asal Malaysia Meninggal Terjatuh Dalam Jurang di Jalur Torean Gunung Rinjani Perjuangan Perajin Bata Merah Selama 15 Tahun Mengantarkannya ke Tanah Suci Beritasatu.com – Setelah mengungkap dugaan perselingkuhannya dengan Ridwan Kamil selebgram Lisa Mariana akhirnya membeberkan lokasi kelahiran anak yang disebut-sebut merupakan hasil hubungan dengan mantan gubernur Jawa Barat tersebut “Anak saya dari Pak RK (Ridwan Kamil) lahir di Pamulang karena dekat dengan tempat tinggal saya,” ujar Lisa Mariana dalam konferensi pers di kawasan Kelapa Gading dirinya sempat mendapat tekanan dari pihak Ridwan Kamil untuk menjalani pemeriksaan kehamilan dan persalinan di rumah sakit pilihan RK “Timnya datang dan memaksa agar proses USG dan persalinan dilakukan di rumah sakit yang ditentukan di Kelapa Gading Saya tolak karena saya takut ada rekayasa,” jelasnya lagi Ridwan Kamil hanya memberikan bantuan uang senilai Rp 15 juta yang diserahkan oleh ajudannya “Uang itu tidak cukup menutupi biaya persalinan saya jadi saya pakai BPJS Kesehatan,” tambahnya semua data kelahiran anaknya tercatat secara resmi di fasilitas kesehatan tempat ia melahirkan dan ajudan RK menjadi pihak yang menandatangani dokumen rumah sakit ajudan Pak RK yang menandatangani surat kelahiran anak saya di Pamulang,” tutup Lisa Mariana yang menyebut melahirkan anak dari hasil hubungan Ridwan Kamil di Pamulang Simak berita dan artikel lainnya di Google News Ikuti yang terbaru di WhatsApp Channel Beritasatu Presiden Prabowo Puji & Sanjung Kinerja Menteri Kabinet Merah Putih Blak-blakan Teman Kuliah Jokowi: Kalau Ijazah Beliau Palsu Magis Tari Kecak Bali Kini Bisa Dinikmati di TMII Jakarta Lonjakan Harga Dorong Tren Investasi Emas Melalui Aplikasi Serangan Tarif Trump dan Erosi Tata Kelola Perdagangan Global Tarif Trump Seret Ekonomi AS ke Jurang Stagflasi Antara TEMPO.CO, Jakarta - The Minister of Energy and Mineral Resources (ESDM), Bahlil Lahadalia, apologized for the long queues to get 3-kilogram liquified petroleum gas (LPG) in the South Tangerang area as this was done solely for restructuring purposes," said Bahlil after inspecting one of the 3-kilogram LPG depots in the Palmerah area of Jakarta on Tuesday Bahlil explained that the government is making policy improvements to prevent the situation from worsening One of the measures he has taken is to change the status of retailers to sub-depots allowing them to sell 3-kilogram LPG again Bahlil is aware that retailers are at the forefront of the 3-kilogram LPG distribution connecting depots with the wider community "What we are doing this morning and tonight is a response We want the people to receive LPG properly and easily," said Bahlil He said that 3-kilogram LPG retailers are now operating again but under the new name of sub-depots The goal of reopening these 3-kilogram LPG retailers is to normalize the distribution channels for the subsidized gas 370,000 retailers have been registered as sub-depots for the 3-kilogram LPG For those retailers who have not yet been registered as sub-depots Bahlil mentioned that the Ministry of Energy and Mineral Resources (ESDM) will actively work with Pertamina to equip them with an application system and assist in the process of becoming sub-depots Bahlil also apologized regarding the news of a resident from RT/RW 001/007 allegedly due to exhaustion after waiting in line to collect a subsidized 3-kilogram LPG in the area on Monday The sad news was shared by the Head of Neighborhood 001, Pamulang Barat, Saeful, who said that Yonih is suspected to have died from exhaustion, which was the primary factor in her death. He explained that the deceased had been waiting in line at the LPG depot the deceased then headed home and took a rest on the way He also mentioned that after realizing the deceased needed medical attention her family took Yonih to the nearest hospital it was later confirmed that she had passed away before reaching the hospital Editor's Choice: Prabowo Orders 3-Kg LPGs to be Sold by Retailers Again Today Click here to get the latest news updates from Tempo on Google News Prabowo Subianto Plans to Establish 100 People's Schools Soon Prabowo Plans to Build Emergency Bulog Warehouses in Aceh, West Nusa Tenggara Prabowo's Reason for Involving the Military in Food Matters Prabowo Open to Meeting Retired Military Forum Urging Gibran's Impeachment Free Meal Poisoning Case, Prabowo Alludes to Students Eating Without Spoons Learn About the Outsourcing Practices Prabowo Intends to Scrap Prabowo: Bill Gates to Support Free Nutritious Meal Program in Indonesia Prabowo Rejects 'Puppet President' Label, Denies Jokowi's Control Manpower Minister Unveils Reasons Behind Over 24,000 Layoffs by April 2025 Prabowo Claims 99.99% Success Rate for Free Nutritious Meal Program Jobs That AI Will Replace: Is Your Current Job at Risk Huayou Replaces LG Energy Solution in Indonesia's EV Project Energy Minister: Indonesia to Operate Nuclear Power Plants by 2030 Palace Denies Cabinet Reshuffle Rumors Following Prabowo-Megawati Meeting BI: Retail Sales Surge in March 2025 Driven by Ramadan Bahlil: Many Countries Are Against Indonesia's Downstreaming Strategy Bahlil Shrugs Off Trump's Tariffs as Normal Global Trade Dynamics Lulu Hypermarket Reportedly Closing: Analyst Says Retail Slump Is Longstanding MR DIY Indonesia Books Rp1.1tn Profit in 2024 The Pope's Role in Major Events of the Modern Era Sistine Chapel Prepares for the Election of New Pope Keanu Reeves and Sandra Bullock Reunite After 30 Years for New Film Project BGN Chief Aims for Zero Accidents in Free Nutritious Meal Program Prabowo Praises Jokowi's Inflation Control in Cabinet Session, Denies Link to Gibran's Presence Inside Sistine Chapel: 5 Key Facts About the Conclave's Iconic Venue Exposing Trump's Move on Mike Waltz Dismissal Jokowi Responds to Calls for VP Gibran's Impeachment Expert Raises Alarm on Crypto Scams, Biometric Leaks After Worldcoin Freeze Today's Top 3 News: 7 Most Beautiful Banknotes in the World, Penetration of Visa, Mastercard vs QRIS in Indonesia Jennie, Lisa, and Rose BLACKPINK Gather at the Met Gala 2025 Israel Approves Plan to Expand Gaza Onslaught, Occupy Territories Indonesia's Mount Semeru Erupts 3 Times This Morning, Sends Ash 700 Meters High WHO: Hand Hygiene is Essential, Medical Gloves Are Not a Substitute List of Baeksang Arts Awards 2025 Winners Menu 0 / 5Saat ini belum ada ulasan untuk Balboa Estate Hillside Buat UlasanHighlights of Balboa Estate HillsideFAQWhat are the exciting facilities that can be found in Balboa Estate Hillside Some attractive facilities in Balboa Estate Hillside include Kamera Keamanan What are the public facilities around Balboa Estate Hillside Some of the public facilities that can be found around Balboa Estate Hillside Where can I see the site plan of Balboa Estate Hillside You can contact the contact person listed on the page or directly explore it in the "Explore Site Plan" section What should I do if I’m interested in buying a unit in Balboa Estate Hillside Our agent will provide you with all the information you need IDR 1,4 Billion - 1,8 BillionBalboa Estate HillsidePamulang Tangerang SelatanGet more informations from similar projectsSelect ALLContact MePermintaan TerkirimPihak Pengembang akan segera menghubungimu untuk informasi lebih lengkap.Kontak PengembangUntuk dapat melakukan komunikasi dengan pengembang silahkan login terlebih dahulu E-commerce dan Platform Online Terbaik BI Awards 2024 Text description provided by the architects has purposes that was more than just a place of worship for Muslims Bioclimatic Community Mosque aims to address the fundamental issues of designing a mosque by distancing itself from the current architectural discussions based on form and focusing solely on the essence of religious space © William SutantoDesign replace the iconic Islamic dome (which mostly a quintessential characteristic of mosques) with a plate of active green roof to cool down top most plates and to reduce enormous urban heat island contributed to the surrounding environment. This were viewed as a movement to redefining Islamic spaces in postmodern context and needs based on accustomed necessity.  the mosque is also designed to blend with nature and local culture As the interior space were basically a shaded outdoor space this was found to be quite effective to tackle the problem of large gathering indoor during airborne pandemic such Covid-19 that happened recently You'll now receive updates based on what you follow Personalize your stream and start following your favorite authors If you have done all of this and still can't find the email State-owned toll operator Jasa Marga announced that President Joko "Jokowi" Widodo is set to inaugurate the Pamulang-Cinere-Bogor Toll Road on Monday This toll road serves as a link between the Jakarta Outer Ring Road (JORR) II Toll Road and the Jakarta-Bogor-Ciawi Toll Road in West Java "After successfully serving the homecoming and return traffic during the Christmas 2023 and New Year 2024 holidays starting from Dec President Joko Widodo will inaugurate the Pamulang-Cinere-Raya Bogor Toll Road on Monday The toll road segments are operated by two toll road business entities which operates the Serpong-Cinere Toll Road section which operates the Cinere-Jagorawi Toll Road section According to the Toll Road Regulatory Agency (BPJT) the construction of the 10.14-kilometer Cinere-Serpong toll road amounts to Rp 2.22 trillion the 14.7-kilometer Cinere-Jagorawi toll road incurs a cost of Rp 3.21 trillion During the preparation for the inauguration of the two toll roads CSJ and TLKJ will partially and temporarily close the Limo Toll Gate on the Cinere-Jagorawi Toll Road on Monday Motorists traveling from Soekarno Hatta Airport Tangerang and nearby areas to the Jagorawi Toll Road/Cijago Toll Road will be redirected to take an alternative route at Limo 1 Toll Gate those traveling from the Jagorawi Toll Road/Cijago Toll Road towards Soekarno Hatta Airport Tangerang and nearby areas will be directed to detour at Limo 2 Toll Gate "Jasa Marga apologizes for any inconvenience experienced during the traffic redirection," Mirza concluded Cari English Inhaling the Smoke from Burning Garbage, Boy Raya Fell Sick TEKS › English›Inhaling the Smoke from.. Iklan Inhaling the Smoke from Burning Garbage Boy Raya Fell Sick If the indiscriminate burning of waste results in the health of residents being compromised residents can sue the person burning the waste Audio Berita This article has been translated using AI. See Original Please note that this article was automatically translated using Microsoft Azure AI, Open AI, and Google Translation AI. We cannot ensure that the entire content is translated accurately. If you spot any errors or inconsistencies, contact us at hotline@kompas.id and we'll make every effort to address them TEKS The following article was translated using both Microsoft Azure Open AI and Google Translation AI underwent treatment for five days at Eka Hospital BSD He suffered from acute respiratory infection or ISPA due to inhaling smoke from garbage burning KOMPAS - Open burning of garbage is one of the triggers for poor air quality which then results in respiratory infections Affected communities can also demand accountability from those who have endangered their health by burning garbage The local government should strengthen its supervision and enforce strict sanctions against garbage burners were rushed to the hospital due to upper respiratory tract infections (URTI) caused by the burning of trash by residents around his house Raya was hospitalized for four days from Saturday (29/7/2023) until Wednesday (2/8/2023) Raya's condition had improved and was allowed to go home in the afternoon so she still needed some medication and further examination by the doctor Also read: Open Burning of Garbage Triggers Deteriorating Air Quality in Jakarta there are often instances of garbage burning by certain individuals in a vacant land behind the South Tangerang General Hospital my child suffered from respiratory distress and had to be taken to the hospital," said Bunga Cempaka Wangi Ranna (34) Bunga thought that Raya was just experiencing a regular cough Raya had difficulty breathing and was taken to the hospital "Raya's breathing became increasingly difficult to the point of gasping there were indentations on the chest and the breathing was getting faster we immediately took her to the hospital," said Bunga He underwent treatment for five days there due to ISPA he suffered Raya was asked to check her blood and a chest X-ray to determine the condition of her lungs Raya was declared to have ISPA because the infection was quite severe Raya's respiratory illness (ISPA) is caused by someone deliberately burning garbage in an empty lot near her house garbage burning can occur three times a week The garbage smoke billows in front of her house and disrupts her activities Bunga stated that the government needs to implement stricter sanctions and closely monitor such activities the individuals involved will not stop if only reprimanded by local residents Read also: Women and Children are Vulnerable to City Air Pollution a study by the World Health Organization (WHO) in 2016 revealed that 93 percent (1.8 billion) of children under the age of 5 in the world breathe highly polluted air WHO also estimated that around 600,000 children in the world died from acute lower respiratory infections caused by polluted air in that year "Bunga stated that the air quality in the South Tangerang area has been poor for the past few months The effect of air pollution is also threatening other children in South Tangerang The burning of garbage by unidentified individuals in an empty land specifically behind the South Tangerang General Hospital (RSU) Raya's schoolmates have been taking turns to skip school due to sickness caused by air pollution I believe that children need extra protection one of which is to keep wearing masks during school," said Bunga Tangerang Selatan was included as the city with the worst air quality in Indonesia and being located in an industrial area are some of the factors causing air pollution in Tangerang Selatan Head of the South Tangerang City Environmental Office acknowledged that the burning of garbage in South Tangerang is indeed a frequent occurrence His team continues to take action against garbage burners and educate them about the dangers of burning waste One of the garbage burners identified was located in the South Tangerang Hospital area Also read: Air Pollution Increases Dementia Risk citizens make use of neglected or disputed land to sort and burn waste that cannot be recycled or does not have any economic value," said Wahyunoto Wahyu stated that their party can only impose administrative sanctions affected residents can legally prosecute waste arsonists Residents burned garbage in an empty land used as a garbage dump in the Pondok Karya area "We need to close the land that has become a source of environmental pollution If there are citizens who are harmed due to burning of waste they can demand legal accountability," he said The criminal sanctions for anyone who intentionally or negligently burns trash and pollutes the environment are regulated in Article 112 of Law Number 32 of 2009 on Environmental Protection and Management Offenders can be imprisoned for up to 1 year or fined up to Rp 500 million along with the high daily visit rates of mosques the architects aimed to design a place of worship the project seeks to perform as more than just a space of faith all images courtesy of RAD+ar in the beginning stages of the project, RAD+ar experimented on ideas for a 100% natural stack ventilation system as well as a 15m breathable wall to envelop the interior space RAD+ar chose to replace 95% of the project’s vertical wall elements with a custom made roster comprised of 30.000 bricks maintaining the interior temperature within 25 to 28 degrees with the minimum wind speed of 1m/s the design made it possible to create a sheltered ‘outdoor-like’ mosque with a dynamic clay roster facade.  the mosque is a sculpture and a benchmark of passive design in religious architecture although the RAD+ar team followed a mainly volumetric approach in the making of the façade the specific characteristics of the region were also taken into account located in the middle of an area with harsh sunlight and high humidity the mosque was formed to be very low maintenance and self-sufficient bioclimatic design was an obvious choice to adopt in order to optimize the use of available solar energy and achieve ample cross-ventilation the perforated facade uses clay as its main material healthy environment with ideal temperature conditions the design replaces the iconic islamic dome with an active green roof plate in order to reduce the enormous urban heat island contributed to the surrounding environment the project redefines islamic spaces within a postmodern context meeting needs based on accustomed necessity with a capacity to accommodate approximately 1,000 people the mosque is designed to blend with nature and local culture, bringing order and harmony to the community the project escapes conventional cultural rules of mosque design formed based on functionality and aesthetics the directional form accentuates movement and the qibla a solemn platform within an assertive form – the internal lighting will create a lantern effect when observed through the exterior facade the breathable facade maintains full privacy for the visitors a dialogue between soul and space – users are left to be in silence and emptiness the custom brick roster facade offers privacy while ensuring 100% natural ventilation – bricks have a low time thermal lag that ensures the facade won’t release heat in the night time name: bioclimatic community mosque of pamulang architecture office: RAD+ar  lead architects: antonius richard designboom has received this project from our ‘DIY submissions‘ feature, where we welcome our readers to submit their own work for publication. see more project submissions from our readers here. edited by: myrto katsikopoulou | designboom AXOR presents three bathroom concepts that are not merely places of function but destinations in themselves — sanctuaries of style On a cool breezy night in early 2023, I paid a return visit to Earhouse, a small café in Pamulang, South Tangerang, where the Earhouse Songwriting Club (ESC) meets weekly. Every Monday, ESC runs a free songwriting class led by a respected independent musician, Endah Widiastuti, from the acoustic duo and married couple, Endah N Rhesa I paid particular attention to the way each class member performed their songs On a small stage - just an area marked by a red carpet - anyone could take their turn singing Everyone was listening carefully and later exchanged comments on each performer’s song Endah usually offers the first impression about songs before others join the discussion got up from his chair and walked to the front The song he was going to play was titled Trauma Irama which was derived from the name of dangdut (popular Indonesian music genre) legend Rhoma Irama while others were still trying to keep up with the lyrics ‘Benalu… Benar-benar tak tahu… Tak tahu malu-malu.’ As he reached the last verse others gently began to sing along Don explained that he sings this one ‘when someone annoys me in the office’ asked Don whether the pitch was too low for him Don immediately changed the song key to match his pitch By now the crowd had grasped the lyrics very well and started to sing along more enthusiastically This is a typical scene on a Monday night at Earhouse I usually watch somewhat nervously from a seat at the back of the room As an undergraduate doing research on music at Earhouse critique and knowledge-sharing speak to me deeply I see these practice sessions as acts of appreciation and community-building But what do they tell us about the wider Indonesian music scene Endah N Rhesa opened Earhouse as a way to connect once more with the local music scene in Pamulang As music lovers increasingly made their way to Earhouse besides hosting a variety of gigs and events Endah established a series of music clubs which attracts many people and has helped to build a vibrant local music scene in Pamulang Earhouse was part of the flourishing of movements in the arts and music in many cities after reformasi (the post-Suharto era in Indonesia) These are spaces with overlapping functions It is usually activated by a community and relates to art Independent from government cultural institutions some scholars have described them as ‘alternative’ and ‘initiative’ spaces One of the characteristics these spaces and communities share is their informality sharing and producing knowledge together and working collaboratively This practice was clear to see in the everyday musical interactions among members of ESC Participants could choose to join the class occasionally without the responsibility to attend every week they would gather outside the café to talk and share more about music The musical processes were seemingly endless ESC emerged as an appreciative community at a time of heightened cynical criticism within the Indonesian music scene led by its cultural gatekeepers such as community front men - senior musicians to casual listeners In our conversations many of my interlocutors told me about their encounters with ‘musical gatekeeping’ before joining ESC Their experiences included negative commentary on their performances a refusal or reluctance to share musical knowledge with each other has a know-it-all attitude and disregards mainstream popular music genres This type of attitude annoys many young people who seek an inclusive space in music as they could easily spot snobbish behaviour through their posts on ‘X’ a content creator who pioneered the idea of the videos helped popularise the word ‘skena’ on the Internet through his Instagram videos These ongoing conversations – both through social media and in real spaces like ESC - highlight a deep-rooted problem in the Indonesian musical landscape, which is the lack of mutual appreciation. This relates to what anthropologist Jeremy Wallach once highlighted as a status consciousness and prestige described by the term ‘gengsi’ Wallach observed that Indonesians had a feeling of prestige toward music that is considered superior Although what is considered superior may change over time gengsi often socially influenced people to exclude certain music from being listened to and discussed From my observations of the music scene such status consciousness or gengsi also forms the basis of many gatekeeping behaviours that neglect the variety of musical cultures By fostering collaborative learning and appreciation as a key practice in the community ESC can be seen as a response to these competing situations It offers a forum to share music ideas without judgement or prejudice and in so doing challenges the subcultural boundaries in Indonesian music Their practices are also part of a wider action as young people and many musicians strive to eradicate stereotypes and gatekeeping in the music industry described the contrast between her experiences here compared to her experiences at a few other arts communities where toxic gatekeeping was still being practised the way participants can access each other’s musical knowledge casually and without intimidation and the support and encouragement they offer each other Vanessa’s reflections are like many I have heard from ESC participants Music is not only about the performer or performance but is also about the audience and listeners ESC practices sparked our sensibility toward being better listeners and encouragement are important gestures in active listening and further build appreciative interactions Cultural infrastructure and institutions are mostly focused on giving people access to perform ESC moves beyond it by expanding their resources to these forms of appreciation ESC is not only a community that romanticises leisure or hobbies It aims for a better future where musicians are well appreciated Arya Adyuta (aryajpadyuta@gmail.com) is studying cultural anthropology at Universitas Gadjah Mada Receive Inside Indonesia's latest articles and quarterly editions in your inbox © Copyright Indonesian Resources and Information Program (IRIP) 1983 - 2025. All rights reserved. We acknowledge the Traditional Custodians of the stolen lands on which Inside Indonesia is based, the Wurundjeri Woi-wurrung people, and we pay our respects to Elders past and present. We recognise that sovereignty was never ceded. Jakarta. Four individuals have been named as suspects in a case involving the alleged assault on Catholic students from Pamulang University (Unpam) who were reciting the Rosary prayer in the Babakan area, Cisauk, Tangerang. Ibnu Bagus Santoso, the South Tangerang Police Chief, revealed that the four suspects are 53-year-old D, 30-year-old I, 36-year-old S, and 26-year-old A. D holds the position of the local neighborhood chief (RT). "The suspect identified as D shouted loudly with profanity and intimidation towards the victims and their friends, with the aim of inciting others to attack them, as they were perceived as disturbing the neighborhood," Ibnu said during a press conference on Tuesday. In addition to D, suspect I also shouted at the students who were praying and physically assaulted them twice. Meanwhile, S and A were reportedly carrying sharp weapons and used them to intimidate the students. "The evidence includes video recordings, three types of knives, a red shirt, and a black shirt," he added. The suspects are facing charges under several articles, including the law on possession of sharp weapons, assault, and ordering others to commit a criminal act. They could face a maximum prison sentence of 10 years. Previously, a video went viral on social media showing Unpam students being subjected to violence while praying the Rosary. In the viral video, several students were surrounded by a crowd, and some were attacked with sharp weapons. One of the female students claimed that local RT officials were involved in the attack.  The incident was uploaded on social media by the account @KatolikG. "Catholic students of Pamulang University gathered at a house in Victor Serpong and prayed the Rosary, but they were besieged by RT officials and residents who brought sharp weapons to disperse and assault the students who were praying. Fortunately, there were no casualties," stated the caption of the video. Text description provided by the architects. Pamulang University has chosen Antonius Richard and RAD+ar to design their new Islamic Oriented public square, plaza building, and public artwork. The project attempts to strengthen the character of its Islamic based development, reconcile cultures and define identities. Octa Plaza will be a focal point by exploring much spatial fun in its 3 dimensional Plaza planning that was displayed. this roundup showcases conceptual interventions that tackle social distancing and the challenges of the pandemic in order to allow people to move freely and safely across space Cari English Karlina Supelli, Embodying Imagination TEKS › English›Karlina Supelli Embodying Imagination Karlina is sad about the things that are happening now the imagination of a better Indonesia must be maintained Audio Berita This article has been translated using AI. See Original TEKS KOMPAS/HERU SRI KUMORO (KUM) 12-04-2019Karlina Supelli Sitting in the back seat, female philosopher and astronomer Karlina Supelli (66) was happy to listen to the research presentation of seven children about Indonesian identity the lecturer at the Driyarkara School of Philosophy also noted down some input for the homeschooled students participating in the Meraba Indonesia Book Club the children aged 10-17 years old read the book Meraba Indonesia: Ekspedisi Gila Keliling Nusantara by Ahmad Yunus but also discussed together every week through the Zoom Meeting communication application "This experience familiarizes children with reading and writing Reading and writing are like a very good pair that should be developed continuously I highly appreciate it," he said in a café in Pamulang Also read: Happy to Feel Indonesian Identity Female philosopher and astronomer Karlina Supelli (second from the left) became a panelist in presenting the results of research by participants of the "Meraba Indonesia Book Club" in Pamulang He also emphasizes the importance of children to continue to imagine everyone will see things in a different way Imagination leads children to delve deeper into the content of reading This experience familiarizes children with reading and writing Reading and writing are like a very good pair to continue to develop Karlina exemplified how the theory of relativity began with Albert Einstein's imagination of riding on light the story of Friedrich August Kekulé von Stradonitz when he revealed the structural formula of benzene Benzene is known by a hexagonal structure resembling a ring Kekulé found himself in front of the fireplace then danced and coiled like a serpent devouring its own tail," he explained Also read: Reflection on the Struggle to Find Karlina Supelli one of which was regarding her imagination about Indonesia Various events happening in Indonesia make her sad the imagination of a better Indonesia for a better life together must still be nurtured He envisions an Indonesia free from corruption and religious and tribal conflict. “I not only have imagination, but also hope. Because, life without hope will stop. "Nurturing imagination and hope while we continue the process and we do everything as little as possible to help make that imagination happen," he said Nurturing imagination and hope while we continue to process and do whatever we can to realize that imagination as much as possible Ini Titik-titik Rawan Banjir di Tangerang Selatan 13 lokasi itu menjadi langganan banjir karena belum memadainya drainase titik rawan banjir sudah jauh berkurang dibanding 2017 Baca juga: Cegah Banjir, Crossing Saluran Akan Dibangun di Jakarta Garden City enggak tergenang lama," ujar Chaeruddin di Kantor Wali Kota Tangerang Selatan Titik banjir diantaranya berada di kawasan Pesanggrahan di Kompleks Deplu Lokasi lain berada di kawasan Ciputat Hilir di Komplek Japos Baca juga: Antisipasi Banjir, SDA Kalideres Angkut Sedimen hingga Siapkan Pompa Mobile Di kawasan Cantiaga berada di Jurang Mangu Permai dan Perumahan Pondok Safari Wali Kota Tangerang Selatan Airin Rachmi Diany mengatakan pihaknya juga melakukan pengerukan kali guna mencegah titik banjir meluas "Kami melakukan pengerukan-pengerukan di kali dan kami terus melakukan pemantauan," ujar Airin Kompas Cyber Media (Kompas Gramedia Digital Group) Baca juga: '+json_baca_juga.items[i].title+' Muhammad Iqbal TEMPO.CO, Jakarta - A South Tangerang Police officer's alleged arrogant behavior including being drunk and extorting street vendors The incident reportedly took place in the Setu District A photo showing a police officer drinking from a bottle suspected to contain alcohol posted on the @portalsulsel social media account has rapidly gained attention.  The caption on the post claims that Chief Brigadier (Bripka) Iwan Lombu was involved in the extortion on February 26 This situation has understandably caused concern among local street vendors Agil confirmed that the officer is currently under investigation "The incident is being handled by the South Tangerang Police's Propam (Internal Affairs Division)," Agil said on Thursday Agil also added that the officer has been placed in a special detention cell "The officer in question is currently being held in a special cell for intensive questioning," he stated He assured the public that the South Tangerang Police do not tolerate misconduct from its members "The South Tangerang Police are committed to taking strict action in accordance with the applicable regulations There is no room for personnel who commit violations," Agil concluded Editor's Choice: Approaching Eid al-Fitr, Counterfeit Money Circulates in Indramayu Click here to get the latest news updates from Tempo on Google News Today's Top 3 News: 7 Most Beautiful Banknotes Bank Indonesia Withdraws Four Banknote Denominations Says Police Behind Violence at Labor Day Rally Jakarta Police Blame Infiltrators for Labor Day Rally Unrest LBH Jakarta Reports Labor Day Protesters Still Detained by Police After Investigation Indonesian Police Bust 7 Online Gambling Sites Israel Wildfire Probe: Police Confirm 3 Arrests How Police Repression Unfolded During Semarang May Day Rally Tempo Journalist Allegedly Assaulted by Police During May Day Rally in Semarang Chaos Erupts at Semarang May Day Rally: Police Arrest Suspected Provocateurs Police Deploy 13,252 Joint Personnel to Safeguard Jakarta May Day Events 5/15/2024 Indonesia (International Christian Concern) — Four Muslim men attacked several Catholic students from Pamulang University as the students finished an evening prayer service on Sunday Around 8 p.m., the leader of the neighborhood where the students were praying approached the students shouting at them and threatening to round up locals to attack them He left and later returned with three others A bystander recorded the incident and shared the footage on social media One student said the neighborhood leader had reprimanded several Catholic students last year and told them that non-Muslims should not be allowed to worship in the area we gave in and did not report it anywhere,” the student said South Tangerang Police have charged four suspects, including the neighborhood leader. South Tangerang Mayor Benyamin Davnie emphasized that members of his community must respect the religious beliefs of others “Events like yesterday should not be repeated in South Tangerang,” Davnie said “There is no place in South Tangerang for intolerance.” For interviews, please email [email protected].  And we know that in all things God works for the good of those who love him who have been called according to his purpose Cari English Users Have Easy Access to Get Hard Drugs in South Tangerang TEKS › English›Users Have Easy Access to Get .. Iklan Users Have Easy Access to Get Hard Drugs in South Tangerang Hard drugs such as Tramadol are still easily accessible by the public freely This is inseparable from the affordable price and the ease of finding sellers of this type of drug Audio Berita This article has been translated using AI. See Original TEKS The following article was translated using both Microsoft Azure Open AI and Google Translation AI. The original article can be found in Pengguna Punya Akses Mudah Mendapatkan Obat Keras di Tangerang Selatan which were confiscated by the Serang Kota Police in Banten were supposed to be only available through a doctor's prescription but are being sold illegally and without regulation KOMPAS - Easy access to obtaining prescription drugs has made some people increasingly free to consume types of drugs that should only be used with a doctor's prescription This ease of access has made the use of prescription drugs without a prescription more widespread Users can easily obtain hard drugs at several stores in the Tangerang Selatan area of Banten province a street vendor in the Pondok Benda area of Pamulang admitted that he can easily get a type of hard drug called Tramadol There are plenty of sellers around here," said AR while pointing out several shops where he usually buys Tramadol along Jalan Parakan Also read: Easy to Buy Tramadol from Jakarta to Tangerang AR showed several locations along Parakan Street Many shops and community centers can be found in this area One of the cosmetic stores that sells hard drugs such as Tramadol in South Jakarta was discovered on Tuesday (29/8/2023) The hard drug was sold without a doctor's prescription "But if the income (when I become a lazy person) is high to be influenced to use these illegal drugs a parking attendant at a shopping center in the Serpong area F usually purchases Tramadol medicine from a cosmetic store on Puspiptek Street He usually buys in the evening or at night He also admitted that he is not worried if there is a raid by the authorities because usually the storekeeper will ensure the safety of the location "The information that I heard is that if the store regularly pays dues to the authorities That's why we feel more at ease buying from stores that are less likely to be inspected," he said A hard medicine pill was found in possession of a shop parking attendant in Serpong shops around Serpong and Pamulang are closed This is due to a raid that took place as a fallout from the murder case of Imam Masykur (25) an Acehnese citizen who was suspected to be a dealer of hard drugs most likely due to the widely publicized murder case involving a Tramadol vendor that has been circulating on social media Also read: Police Arrest Three Civilians Involved in the Murder of Imam Masykur The community living near the location of the sale also harbors suspicions about the transactions of these prescription drugs the owner of a house adjacent to the store that sells prescription drugs is puzzled by a cosmetics store that is dominated by male buyers Evidence of prohibited hard drugs were displayed in a press conference at the Cirebon Kota Police Headquarters in West Java on Monday (9/9/2019) He does not deny that the free sale of hard drugs has become a phenomenon in society Amir is actually surprised that the crackdown on cases seems to be never-ending He is worried that the misuse of such hard drugs could destroy the nation's younger generation Professor of Pharmacy at Gadjah Mada University Using Tramadol can also cause side effects such as nausea the motivation of the users who consume it is not for therapy Note: No forbidden words found in this article to be translated the worst part is that Tramadol addiction can increase the risk of decreased brain function and even death He stated that most users choose to consume this hard drug for other purposes namely to enhance the euphoric effects similar to the use of narcotics the proper use of this type of medication is for relieving pain or discomfort from daily activities the motivation of users who consume it is not for therapy Note: No forbidden words present in this article to be translated State-owned toll road operator Jasa Marga recorded a 28.55 percent increase in total revenue soaring from Rp 16.58 trillion ($1.05 billion) in 2022 to Rp 21.31 trillion in 2023 primarily propelled by increased traffic and newly established toll road sections The company witnessed a 147.37 percent surge in net profit year on year reaching Rp 6.74 trillion compared to the previous year's Rp 2.74 trillion The major share of Jasa Marga's revenue is attributed to toll road operations from Rp 12.44 trillion to Rp 13.94 trillion Further contributing to the revenue was income from other business segments escalating from Rp 1.33 trillion in 2022 to Rp 1.61 trillion in 2023 Head of Corporate Communication and Community Development Group at Jasa Marga elaborated that the EBITDA for 2023 surged by 14.2 percent This upswing is attributed to the commencement of operations on newly established toll road sections enhancing connectivity within the Jasa Marga Group's toll road network "This growth is harmonized and fine-tuned with Jasa Marga's adeptness in managing the expansion of operational costs and ensuring business cost-effectiveness," Lisye said on Monday Jasa Marga reported a surge in the average daily traffic (ADT) on its toll roads The ADT on the Jasa Marga Group toll roads reached 3.5 million vehicles daily reflecting a 5.3 percent increase compared to the ADT of the previous year Maintaining its stronghold as the market leader in the toll road industry Jasa Marga operates toll roads spanning a total length of 1,264 km representing 47 percent of the toll roads in operation across Indonesia This includes the addition of the Serpong-Cinere Toll Road Section 2 from Pamulang to Cinere covering 3.64 km and managed by Cinere Serpong Jaya (CSJ) since December 22 The company owns toll road concessions totaling 1,736 km throughout Indonesia This figure encompasses the latest concession spanning 37.05 km and managed by a subsidiary is actively involved in several ongoing projects including the Jakarta-Cikampek II South Toll Road the Solo-Yogyakarta-NYIA Kulonprogo Toll Road has an unusual hobby: picking up stray dogs off the streets and taking care of them in her house are often bullied and face abuse from neighbors for looking after the dogs and Muslims are not allowed to come in contact with it The 37-year-old met the first stray dog she rescued scrounging for food in a rubbish tip in 2015 My neighbor had to come with me to the tip," Hesti said So I asked my family if I could keep it in the house until it gets better," she said The mother of two said a lot of people still consider stray dogs and cats as "pests," and leave them to die on the streets Animal rights groups say many dogs are stolen from their owners or picked up from the streets to be slaughtered for their meat "I didn't really want to keep them in the house But I can't stand seeing them suffering on the streets," Hesti said Hesti said she once took in seven puppies whose parents had been killed Hesti sells kerupuk (crackers) for a living and has had to sell her motorcycle and other belongings to be able to keep her cats and dogs donations have come from people who saw her posts about her adopted dogs on Facebook Hesti said the posts had also created controversies since many Muslims think she is transgressing Islamic laws by keeping and touching dogs in her house I always purify myself after touching them either by wudu [ritual washing] or by taking a shower," Hesti said She said she never replies to people insulting her and calling her names on social media Hesti's dream is to move to her parents' village near Bogor where she will have more space for the dogs The people there are a lot friendlier to dogs My neighbors here in Pamulang often complain about the noises they make," Hesti said Submit opinion articles or press release to news@tfr.news World Architecture Festival 2021 recently published its shortlists consisting of 200 candidates selected from 700 applicants worldwide Indonesia-based firms RAD+ar and SHAU are among the shortlists RAD+ar is listed under the Office category with its Micro Tropicality building in Jakarta and the Religion category with its Bioclimatic Community Mosque of Pamulang in Tangerang is listed under two categories: Civic & Community and Culture.  World Architecture Festival (WAF) is an annual festival and awards ceremony as well as one of the most prestigious events dedicated to the architecture and development industry the festival carries Resetting the City: Greening The shortlists celebrate completed buildings and landscapes around the world between 2019 and 2021.  the abbreviation for Research Artistic Design + architecture is a group of Indonesian-based architects and designers which focuses on the field of architecture research and development.  Its general concept is to approach architecture with the most provocative and research-based utopian way to tackle urban challenges The Micro Tropicality building is RAD+ar’s explorative response to the local climate and human experience the building challenges various “basic codes” in tropical building design such as installing the facade facing East-West instead of North-South to manipulate internal temperature while retaining rainwater via its roof while minimising the usage of artificial lights at the same time RAD+ar’s Bioclimatic Community Mosque embraces the concept that a mosque is more than just a place of worship The building utilises the sun’s direct heat for solar energy and 12 hours of indirect natural lighting The company also chose to build partition walls using roster blocks instead of bricks to enable optimum air circulation and natural light it uses an active green roof for temperature control throughout the building SHAU is a multinational architecture firm based in the Netherlands The firm was built by experienced architects Florian Heinzelmann SHAU submitted its latest design: Microlibrary Warak Kayu in Semarang which is commissioned by Arkatama Isvara Foundation As described in SHAU’s website the building is elevated like a traditional rumah panggung to add value to the building The diamond-patterned brise-soleils (sunlight deflector) resemble the scaly skin of a local mythical creature called Warak Ngendog This building embodies SHAU’s interest that leans more towards providing design solutions embedded with societal and environmental values The shortlists will compete at the FIL exhibition centre a festival that celebrates the world’s most refined interior designs Jakarta - Jakarta Metro Police confiscated 130 kilograms of marijuana from Suyono Suyono was arrested after the police setup a fake deal South Jakarta Police Spokesman Commissioner Aswin stated that the police took him down as soon as he was on sight Suyono carried along four boxes containing 62 dried marijuana Investigation soon began and led to Suyono's house in Pamulang there were 130 kilograms of ready-to-go dried marijuana," the commissioner said Today's Top 3 News: Police Arrest Indonesia's Drug Cartel Fugitive in the Philippines Police Arrest Indonesia's Drug Cartel Fugitive in the Philippines Govt Assures Indonesian Nationals Safe in Ecuador Amid Rising Gang Violence to Designate Mexican Drug Cartels as Terrorists BNN Depok: Bogor is Center of Marijuana Trafficking Drug Lord's Father In-law Arrested in Mexico   World's Biggest Cocaine Importer on Trial in Italy   Brutal Dog-Drug Smuggling Operation in Italy Uncovered Ricky Mohammad Nugraha Mahinda Arkyasa Jakarta - The food and beverages industry employing people with disabilities is starting to appear in the capital there are at least three coffee shops that employ people with hearing impairment as a barista or a waiter/waitress Other than presenting an economic contribution these coffee shops will certainly hit a soft spot in potential customers' hearts The following is a list of cafes in Jakarta that are made special with the help of disabled people This café located in Pamulang was founded by Disa Ahdanisa in May of 2015 The café that also acts a business specifically empowers people with disabilities in the food and beverage industry was inspired by a café located in Nicaragua The Deaf Cafe Fingertalk currently has two branches with the first at Pamulang and the second is in Limo The business platform that adds social value to the public also received praise from former US President Barack Obama The special element in Disa's cafes is that customers are engaged in understanding sign languages presented by the waiter and waitresses Customers who are not familiar with sign language have nothing to worry since the employees here are able to read lips and certified to do the job The café that literally translates as 'deaf coffee' was founded by three friends; Putri Santoso The difference with this compared to the previously mentioned café is that this café is developed by Putri who is hearing impaired and has a goal of opening job opportunities for people with hearing loss The constant discrimination Putri experienced upon applying for a job was what motivated her to establish the café Putri together with her friends established Kopi Tuli which offers affordable coffee beverages to consumers This café located on RS Fatmawati Street No.15 in South Jakarta was established just two months ago and was founded by Mario Gultom The graduates of a University in Singapore built the café as a business that is based on Socioenterpreneur concept that focuses not only on financial gains but also a business that contributes to the public in its surrounding Sunyi House of Coffee and Hope employs various people with disabilities which are proven by the two baristas who are both paraplegic The strategic location can be an alternative for those living in South Jakarta since it offers quite affordable menus for the food and beverages sold there KOSTCON 2025, the First Largest K-Drama OST Concert in Jakarta, to Take Place on August 2nd 10 Best Cities to Visit in Japan Besides Tokyo to Add to Your Bucket List 10 Recommended Budget-Friendly Tourist Destinations in the United States What Are the Cheapest Businesses to Start from Home? Here Are the Top 10 Hollywood Shaken as Trump Orders 100% Tariff on All Foreign-Made Films Where to Go in May? Here Are the Top 12 Destinations Vesak 2025: Borobudur Temple to Host Thousands of Devotees with Spiritual Celebrations the First Largest K-Drama OST Concert in Jakarta Another Street Brawl Erupts in Jakarta's Manggarai Area Indonesia's Cardinal Suharyo Arrives in Vatican for Papal Conclave Jakarta Civil Servants Told to Take Public Transport on Wednesdays or Face Trouble Pramono Anung Announces Six New Transjabodetabek Routes Set for Launch This Year 2025 Earth Day Action in Jakarta Sees Reduction of Carbon Emissions Coastal Flood Submerges Neighborhood Unit in North Jakarta: BPBD Jakarta Mandates Govt Employees to Use Public Transport on Wednesdays Jakarta Governor: We Will Pursue Vehicle Tax Delinquents Metrics details Mesenchymal stem cell-derived exosomes exhibit promising potential in tissue regeneration Recent studies highlight its significant therapeutic potential in various stages of wound healing the clinical translation of exosome-based therapy was hindered due to issues regarding low productivity and the lack of efficient production protocol to obtain a clinically relevant exosome quantity this study established a potential upscaling protocol to produce exosomes derived from canine adipose-derived mesenchymal stem cells (cAD-MSCs) and explored its potential for wound treatment was carried out using VSCBIC-3 in-house serum-free exosome-collecting solution in a three-dimensional (3D) culture system followed by the tangential flow filtration (TFF) isolation Our findings suggest that culturing cAD-MSCs with VSCBIC-3 maintained cell morphology and viability Compared to conventional two-dimensional (2D) protocols The potential upscaling protocol increased exosome yield and concentration in conditioned medium by 2.4-fold and 3.2-fold The quality assessment revealed enhanced purity and bioactivity of exosomes produced using the VSCBIC-3-3D protocol the cAD-MSCs-derived exosomes were shown to significantly improve fibroblast migration and wound healing-related gene expression in vitro This study collectively demonstrates that potential upscaling protocol establishment allowed robust production of exosomes from cAD-MSCs which exhibit therapeutic potential for wound healing in vitro exosomes isolated from MSCs have been intensively investigated and reported to exhibit therapeutic effects similar to MSCs themselves the use of extracellular vesicles especially exosomes in veterinary study is still developing upscaling exosome production is crucial to enable their broader therapeutic application this study established a potential upscaling protocol for the production of exosomes derived from canine adipose-derived mesenchymal stem cells (cAD-MSCs) This development combined microcarrier-based 3D culture system with TFF isolation method along with the establishment of in-house exosome-collecting medium the wound-healing potential of cAD-MSCs-derived exosomes on fibroblast in vitro was explored This study found increased production yield of cAD-MSCs-derived exosomes produced using the established protocol cAD-MSCs-derived exosomes were evident to enhance the migration and expression of genes related to early wound healing which collectively indicates promising wound treatment potential This establishment is expected to be a robust strategy to produce cAD-MSCs-derived exosomes that are not only beneficial to enhance wound healing but also practical to be applied for veterinary clinical applications B) Morphology representation of isolated cAD-MSCs was observed using phase-contrast microscopy at 40X and 200X magnifications (C) Stemness markers (Oct4 and Rex1) and proliferative marker (Ki67) mRNA expression were assessed via RT-qPCR and normalized to a reference gene (Gapdh) (D) Expression of MSC and hematopoietic-related surface markers was analyzed using Flow cytometry (E) Osteogenic differentiation potency was identified by Alizarin red staining and analysis of gene expression for osteogenic-related markers (F) Chondrogenic differential potency was determined using Alcian blue staining and chondrogenic-related gene expression analysis (G) Adipogenic differential potency was evaluated through Oil Red O staining and the mRNA expression of adipogenic-related markers mRNA expression of multilineage differentiation markers was normalized to the reference gene and the undifferentiated group served as control with asterisks denoting the significance levels (*p < 0.05 These collective findings indicate that the isolated cAD-MSCs exhibit the MSC characteristics and multilineage differentiation potential Establishment of culture medium potential for cAD-MSc exosome collection (B) Morphological representation of cAD-MSCs cultured with various exosome-collecting media investigated at days 1 (C) Live/dead staining of cAD-MSCs upon the culture within various exosome-collecting media Live cells were labeled with green fluorescence using calcein-AM while dead cells were marked with red fluorescence using propidium iodide (D) The viability of cAD-MSCs cultured in different exosome-collecting media was evaluated using the resazurin assay on days 1 The results are presented as fold change normalized to the control group for each day of incubation with asterisks presenting the significance levels compared to the control (*p < 0.05 To preliminarily evaluate the efficiency of the exosome-collecting solutions the morphological appearance of the exosome-producing cells the canine adipose-derived mesenchymal stem cells (cAD-MSCs) maintained under each exosome-collecting solution was observed along with the cell viability as analyzed by live/dead assay and cell population analysis as assessed by resazurin assay The findings suggest that an in-house exosome-collecting solution and population number for at least 3 days which was optimum for the application during the exosome-collecting period The impact of exosome production protocols on both cAD-MSCs characteristics and cAD-MSC-derived exosome qualitative specifications Schematic illustration of (A) 2D-based exosome production protocol and (B) 3D-based exosome production protocol (C) Morphology representation of cAD-MSCs undergoing exosome production was obtained at the beginning (day 0) and the end (day 2) of the conditioning period (D) Live/dead staining of cAD-MSCs was conducted at the beginning and final days of the conditioning period during exosome production Live cells were labeled with calcein-AM dye while dead cells were stained with propidium iodide dye (E) The morphology of cAD-MSC-derived exosomes isolated from different production protocols was visualized using TEM (F) Particle size and distribution and (G) mode size of cAD-MSC-derived exosomes produced from either 2D platforms or 3D platform were analyzed using NTA The value indicating mean was plotted (n = 4) (H) Pseudo-contour diagram generated from Nano-scale flow cytometry analysis illustrating gates of CD9+ exosome produced from different protocols (I) CD9 positivity of exosome was generated from nano-scale flow cytometry findings The results are presented as fold change normalized to the exosome from SF-DMEM-2D as control (J) Zeta potential value of cAD-MSC-derived exosome obtained from various production protocols The asterisks illustrate the significance levels (*p < 0.05 These findings suggest that replacement into the exosome-collecting medium affected the morphology and viability of cAD-MSCs maintained in both 2D and 3D culture systems These collective findings indicate the typical exosome characteristics were detected in samples from all production protocols Quantitative and productivity assessment of cAD-MSC-derived exosome production protocols (A) Schematic workflow of exosome production calculated by total particle production analyzed from NTA divided by initial cell seeding number in various production protocols (n = 4) (C) Comparison of exosome concentration (particle/mL) in conditioned medium of cell cultured using different production protocol (n = 4) (D) The ratio of particle number to microgram protein obtained from different exosome production protocols (E) Pearson’s correlation coefficient between the particle-to-protein ratio and CD9 positivity obtained from exosome characterization across all groups (n = 9) The asterisks indicate the significance levels (*p < 0.05 The aforementioned results suggest similarities in the production profile between SF-DMEM-2D and VSCBIC-3-2D protocols VSCBIC-3-3D protocol demonstrated enhanced exosome production quality and quantity VSCBIC-3-3D exosome production is considered suitable as a potential upscaling protocol In addition to productivity, bioactivity was another essential consideration in developing exosome upscaling production. The bioactivity of exosomes obtained from various production protocols was evaluated by an in vitro migration assay as illustrated in Fig. 5A. cAD–MSC–derived exosomes produced by potential upscaling protocol enhanced the migration of fibroblast in vitro (A) Illustrative diagram depicting in vitro migration assay (B–D) images representing gap closure assessment of cells without or with exosome treatment from various production protocols (E–G) Closure rate analysis illustrates the response of cells to exosome treatment during (E) 0–12 h incubation The data (n = 4) are presented as fold change normalized with the untreated control group in each experimental setup The asterisks suggest the significance levels (*p < 0.05 the higher concentration of VSCBIC-3-3D-exosomes tends to demonstrate increased fibroblast migration While both 5 × 107 particle/mL and 108 particle/mL demonstrated a comparable effect the former at 5 × 107 particle/mL was deemed the cost-effective dose of VSCBIC-3-3D-exosomes These collective findings indicate the beneficial effect of the potential upscaling protocol VSCBIC-3-3D-exosomes were selected to investigate further the phenotypic and genotypic effect of cAD-MSC-exosomes on fibroblasts in vitro Improved Fibroblast Proliferation in vitro induced by cAD-MSC-derived exosomes at various concentrations (A) schematic illustration of the single time point proliferation assay (B) Representative images depicting the morphology and live/dead staining of fibroblasts after 24 h of treatment with different concentrations of exosomes (C) Cell proliferation was assessed using a resazurin assay to evaluate the impact of exosome treatment at varying concentrations during a 24-hour incubation period (D) Schematic workflow of serial time point proliferation assay (E) Representative brightfield images depicting the morphology of fibroblasts at days 1 (F) Live/dead staining of fibroblast co-cultured within varying concentrations of cAD-MSC-derived exosomes over 4 days incubation (G) Cell proliferation analysis compared the effect of exosome treatment at various concentrations over 4-day incubation period normalized with the basal medium control in each day while asterisks indicating the significance levels compared to the control (*p < 0.05 Although no significant difference was observed in the first two days of maintenance that possibly was caused by cellular adaptation cell proliferation significantly increased by day 3 in which the VSCBIC-3-3D exosomes treated groups predominantly showed higher cell proliferation compared to the control despite not being statistically significant These results collectively implied that VSCBIC-3-3D-exosome treatment potentially promotes fibroblast survival and proliferation in vitro The impact of cAD-MSC-derived exosome treatment on fibroblast gene expression in vitro mRNA expression of (A) wound healing-related markers was observed using qRT-PCR after 24 h of treatment (C) Pearson’s correlation analysis across different gene marker expressions related to the effect of cAD-MSC-derived exosome treatment on fibroblast in vitro The asterisks illustrate the significant levels compared to the control (*p < 0.05 Emerging studies have investigated the therapeutic potential of MSC-derived exosomes for various clinical applications including wound healing its translation to clinical practice faces challenges due to the lack of an efficient production protocol to obtain a clinically relevant exosome quantity this study aimed to establish a potential upscaling protocol for exosome production from cAD-MSCs and explore its wound treatment potential in vitro This suggests a potential contamination of apoptotic bodies maintaining cell viability becomes an essential consideration in establishing exosome-collecting medium and ensuring the quality of exosome production VSCBIC-3 in-house exosome-collecting solution was eventually selected in this research to establish a potential upscaling protocol for exosome production This may suggest the potential reproducibility of exosome production from a consistent cell source this approach may lead to scaling out production which is not aligned with our study’s scalability objectives our design controlled key variables in the isolation process to minimize the potential impact of different isolation methods on exosome characteristics we maintained the consistency of the molecular weight cut-off of the membrane and concentration factor of exosome samples derived from different tested protocols These measures ensured that any observed differences in exosome yield and purity were genuinely due to established protocol effects rather than variability in isolation conditions thereby reinforcing the validity of our results Our findings confirmed the exosome production across all groups as evidenced by the presence of typical exosome characteristics in the samples The quantitative profiles of exosome production further revealed similar trends for SF-DMEM-2D and VSCBIC-3-2D protocols suggesting that exosome productivity was not influenced by the choice of conditioned medium the VSCBIC-3-3D protocol demonstrated enhanced productivity marked by 2.4 times higher exosome yield and 3.2-fold increased exosome concentration in the conditioned medium compared to VSCBIC-3-2D protocol This may correspond to the cumulative effect of the microcarrier-based 3D culture system and the TFF isolation method utilized in the VSCBIC-3-3D protocol While the number of cells can indeed affect the overall yield of exosomes our study specifically analyzed exosome yield using the particle-to-cell ratio By normalizing exosome particles to the number of seeding cells in each protocol this indicator reflects exosome production per cell providing a consistent basis for comparison across all protocols our study maintained a constant cell density involved in both 2D and 3D culture conditions allowing reliable comparison of the tested platform Our results reveal a higher particle-to-protein ratio and CD9 positivity attributed to VSCBIC-3-3D-exosomes compared to exosomes obtained from both SF-DMEM-2D and VSCBIC-3-2D This may be associated with lower protein contamination in the sample from the VSCBIC-3-3D-exosomes which highlights the various exosome biogenesis pathways many of which are not reliant on tetraspanins our study encountered some challenges since EV studies have been primarily human-oriented The unavailability of commercially produced anti-dog antibodies tailored for detecting exosome surface antigens limited our examination we aim to expand this approach in future research to further enhance our understanding Our study assessed the bioactivity of exosomes from different production protocols using an in vitro migration assay The findings demonstrated that cAD-MSC-exosomes produced from all given protocols promote the migration effect of fibroblast compared to untreated control similar trends were observed in exosome-induced fibroblast migration for both 2D culture-based protocols either using VSCBIC-3 or SF-DMEM as the collection media the VSCBIC-3-3D exosomes appeared to enhance fibroblast migration The aforementioned evidence possibly contributes to the effects of cAD-MSC-exosome treatment on fibroblasts via autocrine or paracrine pathways the cAD-MSC-exosome effect on enhanced fibroblast activity may correlated to cellular glucose uptake and metabolism which was evidenced by the upregulation of Glut157 Given the wound healing effect of cAD-MSC-exosomes on fibroblast the extended effect of exosome treatment on various cell types and phases involved in wound healing remains to be discovered This may suggest that while the 3D culture system allows enhanced exosome purity this impact may also be influenced by another contributing factor in the VSCBIC-3-3D protocol further study would be in our best interest to examine the altered protein and nucleic acid profile of exosomes produced using our established potential upscaling protocol compared to the 2D culture-based production PBS is considered safe and compatible with clinical standards ensuring the clinical application of our exosome preparation the usage of PBS buffer upon the TEM sample preparation may introduce salt deposition According to the variation observed in the gap closure rate result the following clarification should be noted the in vitro wound healing assay was conducted by maintaining fibroblast cultures in serum-free DMEM as the basal medium omitting the co-founding effect of serum supplementation to evaluate the cell migration and behavior throughout the incubation period the gap closure rate was assessed over three distinct time intervals: 0–12 h This calculation was performed using linear regression in which the wound gap area was plotted against the designated incubation time the slope derived from the regression equation served as the gap closure rate A great variation was observed in the gap closure rate of negative control in the VSCBIC-3-2D group marked by an outlier in the dataset during the 12–24 h incubation time This data point is likely associated with the minimum slope generated from the equation further indicating the low migration rate of the particular replicate during the later stage of incubation This result is possibly attributed to the behavior of the cells upon prolonged serum deprivation This may reflect the challenge for cells to adapt to the nutrient-deficient environment leading to less predictable and high variation migration activity in the subsequent stages of the assay This study was aimed at establishing the fundamental platform for the production of exosome product for clinical application the study’s objectives were set up in two major parts the first is to establish the “potential upscaling protocol” for producing exosomes from cAD-MSCs exploration of the potential application of the cAD-MSCs-derived exosomes produced from the “potential upscaling protocol” for wound healing in vitro For the establishment of the “potential upscaling protocol” for the production of cAD-MSC-derived exosomes The practical upscaling protocol was conducted by using a 3D culture system with microcarrier-assisted culture along with the use of an in-house serum-free exosome collecting medium (VSCBIC-3) This was to ensure that the upscaling protocol was feasible for further pre-industrial-scale production and had the freedom to operate (FTO) according to the patented intellectual property exosomes should be characterized to determine their abundance by evaluating total particle number and/or protein content Although the quantification of these components does not perfectly correlate with exosome numbers it is suggested as a proxy for exosome quantification The analysis to justify the quantification method was recommended to indicate the degree of purity In addition to the quantification of bulk exosome preparation single vesicle analysis allows for the visualization of individual exosomes providing information related to their structure and morphology for ensuring the purity of exosome components The comparison of parameters including exosome morphology expression of exosome-specific surface markers and zeta potential were used to reflect the exosome qualitatively exosome productivity comparison was based on quantitative parameters such as the exosome yield and concentration in a conditioned medium we plan to further investigate the components of cAD-MSC-derived exosomes produced by our upscaling protocol by using the bioinformatics and multi-omics approaches to define the potential active molecules governing the biological activities To explore the potential application of the cAD-MSCs-derived exosomes produced from the potential upscaling protocol for wound healing phenotypic and genotypic analyses were employed This information provided the potential benefits of the exosomes and suggested the potential aspects of further in vivo study models Based on our upscaling production protocol the in-house serum-free exosome collecting medium (VSCBIC-3) along with the exosome collecting process using TFF were used to ensure the quality and purity of exosome components the exosome components in this product were mainly derived from cAD-MSCs’ secretion Our established exosome collection protocol aimed to avoid the isolation of exosomes and soluble particles derived from the serum This approach ensures the purity of exosome preparation providing reliable results portraying exosomes’ utmost efficacy To study the phenotypic properties of cAD-MSC-derived exosomes on wound healing properties fibroblast-based wound healing assays were conducted According to the fibroblast-based wound healing assays it is worth highlighting that fibroblasts are one of the key cell populations governing wound healing involved in multiple stages of the healing process prominently during proliferation and matrix remodeling stages Fibroblast migration and proliferation in the wound site are the key to granulation tissue formation play a pivotal role in regulating collagen deposition and releasing various soluble mediators essential for extracellular matrix remodeling fibroblast-based proliferation and wound-healing (in vitro migration) assays along with the artificial intelligence assistance were used for determining the phenotypic properties of cAD-MSC-derived exosomes on wound healing properties Further genotypic properties were explored to see the dynamic of mRNA marker expression relating to wound healing and help suggested the potential underlying mechanism governing the phenotypic phenomena These promising results lay the groundwork for subsequent research phases our study further aims to advance to subsequent stages for this development which may involve in vivo pre-clinical studies and ultimately step 3 regarding clinical trials it is evident that the established potential upscaling protocol allowed the enhanced exosome production and bioactivity of cAD-MSC-exosomes This protocol establishment involves the establishment of an exosome-collecting medium and microcarrier-based 3D-culture system Our comparative analysis exhibits the enhanced effect of cAD-MSC-exosomes produced by this platform on fibroblast in vitro adipose tissue samples were collected under anesthesia from the abdominal fat of each donor (n = 4) The donor inclusion criteria included healthy dogs aged between 10 months and 5 years and the participation was contingent upon the owner’s consent The tissue was washed with Phosphate Buffer Saline (PBS Thermo Fisher Scientific) containing 2% Antibiotic–Antimycotic (Thermo Fisher Scientific) followed by mincing and incubating within Cell Recovery Solution (Corning) for 2 h at 37 °C The mixture was subsequently filtered through a 70 μm Falcon™ Cell Strainers (Corning) followed by washing using PBS and centrifuged at 600 g for 5 min The collected cells were resuspended and seeded onto a 100 mm-culture dish (Corning) utilizing routine used growth medium consisting of Dulbecco’s Modified Eagle Medium (DMEM and 1% Antibiotic–Antimycotic (Thermo Fisher Scientific) at 37 °C in a humidified incubator with 5% CO2 Cell morphology was examined under a phase-contrast microscope (EVOS™ Core Imaging System microscope; Thermo Fisher Scientific) The mRNA expressions related to stemness makers (Rex1 and Oct4) and proliferative marker (Ki67) were analysed by means of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) Surface marker expression related to MSCs (CD73 and CD44) and hematopoietic cells (CD45) was examined by flow cytometry Staining was performed using specific antibodies namely PE-conjugated mouse anti-human CD29 monoclonal antibody (BioLegend) PE-conjugated rat anti-dog CD90 monoclonal antibody (eBioscience) Alexa Fluor 488-conjugated rat anti-dog CD44 antibody (BioRad) mouse anti-human CD73 monoclonal antibody (Thermo Fisher Scientific) and FITC-conjugated goat anti-mouse immunoglobulin G (IgG) as the secondary antibody (BioRad) FITC-conjugated mouse anti-human CD45 monoclonal antibody (BioLegend) While isotype controls used in this study were PE-conjugated mouse IgG1 kappa Isotype (BioLegend) PE-conjugated rat IgG2b kappa Isotype (eBioscience) Alexa Fluor 488-conjugated rat IgG2a Isotype (BioRad) FITC-conjugated mouse IgG1 kappa Isotype (BioLegend) mouse IgG2a kappa Isotype (Thermo Fisher Scientific) The analysis was performed using the BD FACSCalibur Flow Cytometer (BD Bioscience) Osteogenic differentiation protocol commenced by seeding 2.5 × 104 cells onto 24-well culture plate followed by maintenance using osteogenic induction medium containing routine used growth medium supplemented with 50 mg/mL ascorbic acid (Sigma) and 10 mM β-glycerophosphate (Sigma) for 14 days The mineralization of ECM was detected using Alizarin Red staining (Sigma) The expression of osteogenic-related markers For the chondrogenic differentiation protocol cells were seeded on 24-well plates (Corning) within 5 × 104 cell/well density and maintained in a chondrogenic induction medium containing DMEM (Thermo Fisher Scientific) supplemented with 15% FBS (Thermo Fisher Scientific) and 10 ng/mL transforming growth factor beta 3 (TGF-β3 The formation of glycosaminoglycan was analyzed using Alcian Blue staining (Sigma) after 21 days of maintenance The mRNA expression of chondrogenic-related markers (Sox9 and Col2a1) was assessed using RT-qPCR the adipogenic differentiation protocol was conducted for 28 days Cells were seeded on 24-well plates (Corning) within 3 × 104 cell/well density and maintained in adipogenic induction medium containing growth medium supplemented with 1 µM dexamethasone (Sigma) and 0.1 mg/mL insulin (Sigma-Aldrich) for 72 h followed by 24 h maintenance in adipogenic maintenance medium consist of growth medium supplemented with 0.1 mg/mL insulin Those induction cycles were subjected to up to 5 cycles following maintenance until 28 days in total the generated intracellular lipid droplets were detected using Oil Red O staining (Sigma) and the expression of adipogenic-related markers utilized in this study consisted of DMEM supplemented 1% GlutaMAX™ (Thermo Fisher Scientific) and 1% Antibiotic–Antimycotic (Thermo Fisher Scientific) which was also used as a group comparison to the established in-house media Various exosome-collecting media were chemically defined and tailored based on our prior unpublished investigation These modifications included different supplementation of the basal medium as follows: (i) exosome-depleted-FBS supplemented medium (dEx-DMEM) consisting of basal medium supplemented with 5% of in-house exosome-depleted-FBS (ii) in-house exosome-collecting solution (VSCBIC-3) consisted of basal medium modified by the addition of chemically defined supplements in which the exact formulation cannot be disclosed due to the confidential contract (iii) exosome-depleted-FBS supplemented in-house exosome-collecting solution (dEx-VSCBIC-3) consist of in-house VSCBIC-3 solution supplemented with 5% of in-house exosome-depleted-FBS a routine growth medium was used as a control in this study The qualitative analysis of cell viability was assessed using live/dead double staining at 1 The cells were washed with sterile PBS and incubated in a serum-free medium containing 1 µg/mL calcein-AM and 1 µg/mL propidium iodide for 20 min The evaluation was performed using a confocal microscope system (AX / AX R with NSPARC The concentrate was collected and subsequently diafiltrated using PBS (Sigma) and eventually filtered through a 0.22 μm sterile syringe filter unit (Thermo Fisher Scientific) The isolated exosomes were then stored at -80 °C for further experiments followed by diafiltration using PBS (Thermo Fisher Scientific) exosomes were filtered through a 0.22 μm sterile syringe filter unit (Thermo Fisher Scientific) and stored at -80 °C for further experiment 3T3-J2 fibroblasts was seeded in a 24-well plate at a density of 5 × 104 cells/well and incubated at 37 °C until reaching a monolayer confluency a scratch was generated at the center of each well using SPLScar™ Scratcher (SPL Life Science) and washed with PBS (Sigma) to eliminate debris The cells were then co-cultured with exosomes produced from various protocols (at concentrations of 106 while cells maintained in serum-free basal medium was served as untreated control The gap closure process was observed at multiple time points (0 and 24 h) using a brightfield microscope (confocal microscope system AX / AX R with NSPARC closure rate analysis and comparisons were conducted using NIS-Elements Advanced Research (Nikon) 3T3-J2 fibroblasts were seeded on 96-well plate within 5 × 103 cells per well density and allowed to adhere for 24 h The cells were then co-cultured with VSCBIC-3-3D-exosomes at concentrations of 106 while cells cultured in serum-free basal medium was subjected as untreated control group Resazurin assay was performed by adding a solution of resazurin (Sigma) to each well until reaching a final concentration of 0.015 mg/mL within the medium The absorbance was measured using a spectrophotometer at wavelengths of 570 nm and 600 nm to examine the long-term effect of exosome therapy serial time point proliferation assay was carried out DMEM supplemented with 3.5% FBS (Sigma) served as the basal medium and untreated control group 3T3 fibroblasts were co-cultured with or without VSCBIC-3-3D-exosomes at various concentrations The mRNA expression was normalized using Glyceraldehyde 3-phosphate dehydrogenase (Gapdh) for canine cells and ribosomal Protein L13 (Rlp13A) for mouse cells Relative mRNA value was calculated using the 2−ΔΔt method by comparing it to the control group The generated data was plotted and statistically analysed using RStudio (Posit Software version 2023.06.1 + 524) and presented as mean and standard deviation (mean ± SD) Kruskal Wallis test was used to compare more than two groups of samples Man-Whitney U test was utilized for pairwise comparisons between two groups the correlation between two different variables was calculated using Pearson’s correlation coefficients The p-value < 0.05 was considered statistically significant The datasets used and/or analyzed during the current study available from the corresponding author on reasonable request Prasai, A., Jay, J. W., Jupiter, D. & Wolf, S. E. El Ayadi, A. Role of exosomes in dermal wound healing: A systematic review. J. Invest. Dermatology. 142, 662–678e668. https://doi.org/10.1016/j.jid.2021.07.167 (2022) Ayavoo, T., Murugesan, K. & Gnanasekaran, A. Roles and mechanisms of stem cell in wound healing. Stem Cell. Investig. 8, 4. https://doi.org/10.21037/sci-2020-027 (2021) Zeng, Q. L. & Liu, D. W. Mesenchymal stem cell-derived exosomes: an emerging therapeutic strategy for normal and chronic wound healing. World J. Clin. Cases. 9, 6218–6233. https://doi.org/10.12998/wjcc.v9.i22.6218 (2021) Dai, W. et al. Microenvironmental cue-regulated exosomes as therapeutic strategies for improving chronic wound healing. NPG Asia Mater. 14 https://doi.org/10.1038/s41427-022-00419-y (2022) Vu, N. B. et al. Stem cell-derived exosomes for wound healing: current status and promising directions. Minerva Med. 112, 384–400. https://doi.org/10.23736/s0026-4806.20.07205-5 (2021) Katsha, A. M. et al. Paracrine factors of multipotent stromal cells ameliorate lung injury in an Elastase-induced emphysema model. Mol. Ther. 19, 196–203. https://doi.org/10.1038/mt.2010.192 (2011) Li, D. & Wu, N. Mechanism and application of exosomes in the wound healing process in diabetes mellitus. Diabetes Res. Clin. Pract. 187, 109882. https://doi.org/10.1016/j.diabres.2022.109882 (2022) He, X. et al. MSC-Derived Exosome Promotes M2 Polarization and Enhances Cutaneous Wound Healing. Stem Cells Int 7132708, (2019). https://doi.org/10.1155/2019/7132708 (2019) Gupta, D., Zickler, A. M. & El Andaloussi, S. Dosing extracellular vesicles. Adv. Drug Deliv. Rev. 178, 113961. https://doi.org/10.1016/j.addr.2021.113961 (2021) Cao, J. et al. Three-dimensional culture of MSCs produces exosomes with improved yield and enhanced therapeutic efficacy for cisplatin-induced acute kidney injury. Stem Cell. Res. Ther. 11, 206. https://doi.org/10.1186/s13287-020-01719-2 (2020) Rezaie, J., Feghhi, M. & Etemadi, T. A review on exosomes application in clinical trials: perspective, questions, and challenges. Cell. Commun. Signal. 20, 145. https://doi.org/10.1186/s12964-022-00959-4 (2022) Haraszti, R. A. et al. Exosomes produced from 3D cultures of MSCs by tangential flow filtration show higher yield and improved activity. Mol. Ther. 26, 2838–2847. https://doi.org/10.1016/j.ymthe.2018.09.015 (2018) Casajuana Ester, M. & Day, R. M. Production and utility of extracellular vesicles with 3D culture methods. Pharmaceutics 15 https://doi.org/10.3390/pharmaceutics15020663 (2023) Jonkman, J. E. N. et al. An introduction to the wound healing assay using live-cell microscopy. Cell Adhes. Migr. 8, 440–451. https://doi.org/10.4161/cam.36224 (2014) Dominici, M. et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement. Cytotherapy 8, 315–317. https://doi.org/10.1080/14653240600855905 (2006) Dang Le, Q. et al. In vitro generation of transplantable insulin-producing cells from canine adipose-derived mesenchymal stem cells. Sci. Rep. 12, 9127. https://doi.org/10.1038/s41598-022-13114-3 (2022) Rodprasert, W. et al. Tailored generation of insulin producing cells from canine mesenchymal stem cells derived from bone marrow and adipose tissue. Sci. Rep. 11, 12409. https://doi.org/10.1038/s41598-021-91774-3 (2021) Nantavisai, S. et al. Systems biology analysis of osteogenic differentiation behavior by canine mesenchymal stem cells derived from bone marrow and dental pulp. Sci. Rep. 10, 20703. https://doi.org/10.1038/s41598-020-77656-0 (2020) Ivanovska, A. et al. Immunophenotypical characterization of canine mesenchymal stem cells from perivisceral and subcutaneous adipose tissue by a species-specific panel of antibodies. Res. Vet. Sci. 114, 51–58. https://doi.org/10.1016/j.rvsc.2017.02.019 (2017) Extensive characterization of feline intra-abdominal adipose-derived mesenchymal stem cells Humenik, F. et al. A comparative study of canine mesenchymal stem cells isolated from different sources. Anim. (Basel). 12 https://doi.org/10.3390/ani12121502 (2022) Purbantoro, S. D., Osathanon, T., Nantavisai, S. & Sawangmake, C. Osteogenic growth peptide enhances osteogenic differentiation of human periodontal ligament stem cells. Heliyon 8, e09936. https://doi.org/10.1016/j.heliyon.2022.e09936 (2022) Davies, R. et al. Extracellular vesicle depletion protocols of foetal bovine serum influence umbilical cord mesenchymal stromal cell phenotype, Immunomodulation, and particle release. Int. J. Mol. Sci. 24, 9242. https://doi.org/10.3390/ijms24119242 (2023) Li, J. et al. Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles. J. Extracell. Vesicles. 4, 26883. https://doi.org/10.3402/jev.v4.26883 (2015) Giannasi, C. et al. Serum starvation affects mitochondrial metabolism of adipose-derived stem/stromal cells. Cytotherapy 25, 704–711. https://doi.org/10.1016/j.jcyt.2023.03.004 (2023) Man, K., Eisenstein, N. M., Hoey, D. A. & Cox, S. C. Bioengineering extracellular vesicles: smart nanomaterials for bone regeneration. J. Nanobiotechnol. 21, 137. https://doi.org/10.1186/s12951-023-01895-2 (2023) Koh, B. et al. A Three-Dimensional Xeno-Free Culture Condition for Wharton’s Jelly-Mesenchymal Stem Cells: The Pros and Cons. Int. J. Mol. Sci. 24 https://doi.org/10.3390/ijms24043745 (2023) Ferrari, C. et al. Limiting cell aggregation during mesenchymal stem cell expansion on microcarriers. Biotechnol. Prog. 28, 780–787. https://doi.org/10.1002/btpr.1527 (2012) Takahashi, I., Sato, K., Mera, H., Wakitani, S. & Takagi, M. Effects of agitation rate on aggregation during beads-to-beads subcultivation of microcarrier culture of human mesenchymal stem cells. Cytotechnology 69, 503–509. https://doi.org/10.1007/s10616-016-9999-5 (2017) Hanga, M. P. et al. Scale-up of an intensified bioprocess for the expansion of bovine adipose-derived stem cells (bASCs) in stirred tank bioreactors. Biotechnol. Bioeng. 118, 3175–3186. https://doi.org/10.1002/bit.27842 (2021) Syromiatnikova, V., Prokopeva, A. & Gomzikova, M. Methods of the Large-Scale production of extracellular vesicles. Int. J. Mol. Sci. 23 https://doi.org/10.3390/ijms231810522 (2022) Paolini, L. et al. Large-scale production of extracellular vesicles: report on the massivevs ISEV workshop. J. Extracell. Biology. 1, e63. https://doi.org/10.1002/jex2.63 (2022) de Fuzeta, A. Scalable production of human mesenchymal stromal Cell-Derived extracellular vesicles under Serum-/Xeno-Free conditions in a Microcarrier-Based bioreactor culture system. Front. Cell. Dev. Biol. 8, 553444. https://doi.org/10.3389/fcell.2020.553444 (2020) Koh, B. et al. Three dimensional microcarrier system in mesenchymal stem cell culture: a systematic review. Cell. Biosci. 10, 75. https://doi.org/10.1186/s13578-020-00438-8 (2020) Jeske, R. et al. Upscaling human mesenchymal stromal cell production in a novel vertical-wheel bioreactor enhances extracellular vesicle secretion and cargo profile. Bioactive Mater. 25, 732–747. https://doi.org/10.1016/j.bioactmat.2022.07.004 (2023) Muok, L. et al. Extracellular vesicle biogenesis of three-dimensional human pluripotent stem cells in a novel Vertical-Wheel bioreactor. J. Extracell. Biology. 3, e133. https://doi.org/10.1002/jex2.133 (2024) Debbi, L., Guo, S., Safina, D. & Levenberg, S. Boosting extracellular vesicle secretion. Biotechnol. Adv. 59, 107983. https://doi.org/10.1016/j.biotechadv.2022.107983 (2022) Yuan, X. et al. Engineering extracellular vesicles by three-dimensional dynamic culture of human mesenchymal stem cells. J. Extracell. Vesicles. 11, e12235. https://doi.org/10.1002/jev2.12235 (2022) Busatto, S. et al. Tangential flow filtration for highly efficient concentration of extracellular vesicles from large volumes of fluid. Cells 7 https://doi.org/10.3390/cells7120273 (2018) Patel, D. B. et al. Impact of cell culture parameters on production and vascularization bioactivity of mesenchymal stem cell-derived extracellular vesicles. Bioeng. Transl Med. 2, 170–179. https://doi.org/10.1002/btm2.10065 (2017) Barekzai, J. et al. Process development for the production of mesenchymal stromal cell-derived extracellular vesicles in conventional 2D systems. Cytotherapy 26, 999–1012. https://doi.org/10.1016/j.jcyt.2024.04.071 (2024) Bader, J., Narayanan, H., Arosio, P. & Leroux, J. C. Improving extracellular vesicles production through a bayesian optimization-based experimental design. Eur. J. Pharm. Biopharm. 182, 103–114. https://doi.org/10.1016/j.ejpb.2022.12.004 (2023) Kim, J. Y. et al. Defined MSC exosome with high yield and purity to improve regenerative activity. J. Tissue Eng. 12, 20417314211008626. https://doi.org/10.1177/20417314211008626 (2021) Welsh, J. A. et al. Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches. J. Extracell. Vesicles. 13, e12404. https://doi.org/10.1002/jev2.12404 (2024) Ciftci, E. et al. Comparative analysis of magnetically activated cell sorting and ultracentrifugation methods for exosome isolation. PLoS One. 18, e0282238. https://doi.org/10.1371/journal.pone.0282238 (2023) Furuta, T. et al. Mesenchymal stem Cell-Derived exosomes promote fracture healing in a mouse model. Stem Cells Transl Med. 5, 1620–1630. https://doi.org/10.5966/sctm.2015-0285 (2016) Hu, L. et al. Exosomes derived from human adipose mensenchymal stem cells accelerates cutaneous wound healing via optimizing the characteristics of fibroblasts. Sci. Rep. 6, 32993. https://doi.org/10.1038/srep32993 (2016) Oh, E. J. et al. Extracellular vesicles derived from fibroblasts promote wound healing by optimizing fibroblast and endothelial cellular functions. STEM CELLS. 39, 266–279. https://doi.org/10.1002/stem.3310 (2021) Barrientos, S., Brem, H., Stojadinovic, O. & Tomic-Canic, M. Clinical application of growth factors and cytokines in wound healing. Wound Repair. Regen. 22, 569–578. https://doi.org/10.1111/wrr.12205 (2014) Gilbert, R. W. D., Vickaryous, M. K. & Viloria-Petit, A. M. Signalling by transforming growth factor Beta isoforms in wound healing and tissue regeneration. J. Dev. Biol. 4 https://doi.org/10.3390/jdb4020021 (2016) Wang, L. et al. Exosomes secreted by human adipose mesenchymal stem cells promote scarless cutaneous repair by regulating extracellular matrix remodelling. Sci. Rep. 7, 13321. https://doi.org/10.1038/s41598-017-12919-x (2017) Jiang, T., Wang, Z. & Sun, J. Human bone marrow mesenchymal stem cell-derived exosomes stimulate cutaneous wound healing mediates through TGF-beta/Smad signaling pathway. Stem Cell. Res. Ther. 11, 198. https://doi.org/10.1186/s13287-020-01723-6 (2020) TNF-alpha stimulation of fibroblast proliferation Dependence on platelet-derived growth factor (PDGF) secretion and alteration of PDGF receptor expression Ritsu, M. et al. Critical role of tumor necrosis factor-α in the early process of wound healing in skin. J. Dermatology Dermatol. Surg. 21, 14–19. https://doi.org/10.1016/j.jdds.2016.09.001 (2017) Chen, X. & Thibeault, S. L. Role of tumor necrosis factor-alpha in wound repair in human vocal fold fibroblasts. Laryngoscope 120, 1819–1825. https://doi.org/10.1002/lary.21037 (2010) Oh, S., Kim, H., Nam, K. & Shin, I. Glut1 promotes cell proliferation, migration and invasion by regulating epidermal growth factor receptor and integrin signaling in triple-negative breast cancer cells. BMB Rep. 50, 132–137. https://doi.org/10.5483/bmbrep.2017.50.3.189 (2017) Kawai-Harada, Y., Nimmagadda, V. & Harada, M. Scalable isolation of surface-engineered extracellular vesicles and separation of free proteins via tangential flow filtration and size exclusion chromatography (TFF-SEC). BMC Methods. 1 https://doi.org/10.1186/s44330-024-00009-0 (2024) Kim, H. et al. Comprehensive molecular profiles of functionally effective MSC-Derived extracellular vesicles in Immunomodulation. Mol. Ther. 28, 1628–1644. https://doi.org/10.1016/j.ymthe.2020.04.020 (2020) Jarmalaviciute, A., Tunaitis, V., Pivoraite, U., Venalis, A. & Pivoriunas, A. Exosomes from dental pulp stem cells rescue human dopaminergic neurons from 6-hydroxy-dopamine-induced apoptosis. Cytotherapy 17, 932–939. https://doi.org/10.1016/j.jcyt.2014.07.013 (2015) Camoes, S. P. et al. 3D-MSCs A151 ODN-loaded exosomes are Immunomodulatory and reveal a proteomic cargo that sustains wound resolution. J. Adv. Res. 41, 113–128. https://doi.org/10.1016/j.jare.2022.01.013 (2022) Cheng, K. & Kalluri, R. Guidelines for clinical translation and commercialization of extracellular vesicles and exosomes based therapeutics. Extracell. Vesicle. 2, 100029. https://doi.org/10.1016/j.vesic.2023.100029 (2023) Rikkert, L. G., Nieuwland, R., Terstappen, L. & Coumans, F. A. W. Quality of extracellular vesicle images by transmission electron microscopy is operator and protocol dependent. J. Extracell. Vesicles. 8, 1555419. https://doi.org/10.1080/20013078.2018.1555419 (2019) Iwanicki, M. et al. PDZ-RhoGEF and ROCKII cooperate to regulate adhesion movement and trailing-edge Retraction in fibroblasts. J. Cell Sci. 121, 895–905. https://doi.org/10.1242/jcs.020941 (2008) U.S. Food and Drug Administration. The Drug Development Process, (2018). https://www.fda.gov/patients/learn-about-drug-and-device-approvals/drug-development-process Zhang, J. et al. Exosomes derived from human endothelial progenitor cells accelerate cutaneous wound healing by promoting angiogenesis through Erk1/2 signaling. Int. J. Biol. Sci. 12, 1472–1487. https://doi.org/10.7150/ijbs.15514 (2016) Jeske, R. et al. Agitation in a Microcarrier-based spinner flask bioreactor modulates homeostasis of human mesenchymal stem cells. Biochem. Eng. J. 168 https://doi.org/10.1016/j.bej.2021.107947 (2021) Kim, M. K., Choi, Y. C., Cho, S. H., Choi, J. S. & Cho, Y. W. The antioxidant effect of small extracellular vesicles derived from Aloe vera peels for wound healing. Tissue Eng. Regen Med. 18, 561–571. https://doi.org/10.1007/s13770-021-00367-8 (2021) Midekessa, G. et al. Zeta potential of extracellular vesicles: toward Understanding the attributes that determine colloidal stability. ACS Omega. 5, 16701–16710. https://doi.org/10.1021/acsomega.0c01582 (2020) Download references was supported by Chulalongkorn University’s Graduate Scholarship Program for ASEAN or Non -ASEAN Countries and the 90th Anniversary Chulalongkorn University Fund this research is funded by Thailand Science Research and Innovation Fund Chulalongkorn University Center of Excellence for Regenerative Dentistry Chulalongkorn University for providing access to spectrofluorometer instrument; and the Center of Excellence for Veterinary Clinical Stem Cells and Bioengineering Chulalongkorn University for allowing access to the research facility The International Graduate Program of Veterinary Science and Technology (VST) Yudith Violetta Pamulang & Irma Padeta Center of Excellence for Veterinary Clinical Stem Cells and Bioengineering Chutirat Torsahakul & Chenphop Sawangmake Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC) Noppadol Sa-Ard-lam & Rangsini Mahanonda Center of Excellence in Periodontal Disease and Dental Implant Center of Excellence in Regenerative Dentistry designed and performed the majority of the experiments; collected and interpreted the data; and wrote the manuscript provided the ideas and designed the exosome production protocols provided the analysis and interpretation of data provided substantial revision to the manuscript conceived the main ideas; designed the experiments; analyzed and interpreted the data; and wrote and revised the manuscript The authors declare no competing interests Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Below is the link to the electronic supplementary material Download citation DOI: https://doi.org/10.1038/s41598-025-93219-7 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing newsletter — what matters in science 4/29/2025 Indonesia (International Christian Concern) — After more than three decades of worshiping in limited social facilities, the Huria Kristen Batak Protestant (HKBP) Pamulang Resort Ciputat Banten District finally got a permit to build its church building. The permit to build a house of worship has now been granted by residents and the local government The news was announced to the congregation during the delivery of God’s Word during the Palm Sunday Service on April 13. The congregation has been worshiping in the Social Facility Building while waiting for the government to grant its building permit HKBP Pamulang has begun construction of a church building next to its previous worship facility With the local community’s blessing and the local government’s approval HKBP Pamulang can now continue building a long-awaited house of worship This is the answer to the prayers and hopes that we have prayed for years,” said Rev He added that the congregation’s joy is great and they are now preparing for the next stages of construction The hope is that this church can get support from various parties to support the construction of the building which is planned to be used in late 2025 or early 2026 To read more news stories, visit the ICC Newsroom. For interviews, please email [email protected].  And we know that in all things God works for the good of those who love him, who have been called according to his purpose. ShareGet SBS News daily and direct to your InboxSign up now for the latest news from Australia and around the world direct to your inbox.Your email address *Morning (Mon–Fri)