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She was one of 11 siblings whom she cherished and adored
Francisca enjoyed traveling the country with her husband
and her many hobbies that included needlework
Cantu is preceded in death by her loving husband
Donald Romero and Monico “Chuck” Vega; and daughter-in-law
Amalia Vega and Aurora (Tim) O’Toole; 11 grandchildren; many great grandchildren and great-great grandchildren
Francisca is also survived by numerous close relatives
Funeral service will take place at 9:30 a.m
Interment will follow at Rio Grande Valley State Veterans Cemetery in Mission
The family of Francisca would like to thank the care providers at Villa Pueblo for their continuous care and a very heartfelt thanks to Molly Duran for her companionship and friendship to Francisca while at Villa Pueblo
The family would also like to thank the entire family and staff
Funeral services are under the direction of Memorial Funeral Home in San Juan
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a multi-vehicle collision occurred around 9 p.m
on Highway 101 south of Miraposa Reina in Gaviota
Five vehicles were involved in the incident
The first northbound and southbound lanes were closed
One adult female patient was transported by ambulance with moderate injuries to Santa Barbara Cottage Emergency Room
Officials reported heavy traffic in the area
The cause of the crash is still under investigation with the California Highway Patrol
PEP.ph has been granted the NPC Seal of Registration in recognition of the successful registration of its DPO and DPS
Filipino pageant enthusiasts rejoiced after Filipina beauty queen Dia Maté bagged the Reina Hispanoamericana 2025 title
Dia stood out among 23 contestants from various countries with Hispanic influences during the pageant's grand finale on Monday
She became the successor of Maricielo Gamarra of Peru and the second Filipina to clinch this title
following Winwyn Marquez's win in 2017
Read: Philippine bet Dia Mate wins Reina Hispanoamericana 2025
the spotlight is now on the Philippines' second "Latina Slayer." Here's everything you need to know about Reina Hispanoamericana 2025 Dia Mate:
Dia is the daughter of business and marketing professionals Lea and Mike Mate
She hails from the influential Remulla political clan of Cavite and is the granddaughter of Justice Secretary Jesus Crispin “Boying” Remulla
Dia is in a relationship with OPM singer Juan Karlos Labajo
she took to Instagram to post a heartfelt birthday message for her boyfriend
whom she lovingly referred to as her "best friend" and "greatest love."
"Happiest birthday to my best friend and my greatest love [heart emoji] You deserve the world and more."
Read: JK Labajo congratulates gf Dia Mate for winning Reina crown
Juan Karlos officially announced their relationship on Valentine's Day
Read: JK Labajo Shares Sweet Pic with Dia Mate on Valentine's
making her debut in May 2024 at the Miss Universe Philippines pageant
but this didn't deter her from becoming a beauty queen
she joined the Miss World Philippines and was crowned Reina Hispanoamericana Filipinas 2024
A highlight of her latest pageant journey was Dia's Best in National Costume win for her stunning "Banaag" ensemble
who drew inspiration from the ancient Baroque churches of the Philippines
acknowledged as an architectural treasure by UNESCO
Dia also dazzled in a vibrant carnival costume named "Enero," designed by Er Stephen Alvarado
who used the grand festivals of January—like Sinulog
Dia radiated elegance in a gold gown created by Rian Fernandez
Dia made a lasting impression during the question-and-answer segment when the judges asked her about the values she considers essential for building a fair society and their significance
"I think the most important value that we should have is kindness
Latinas have shown me so much kindness and so much love even though racially I am not Latino
"And the most beautiful thing I've noticed is that even though we don't speak the same language
"And I hope this shows everybody that if we use kindness we can show that we are all the same and can create a better world and a better society for us all."
Dia actively promotes mental health awareness and advocates for music therapy as a helpful means of healing mental illnesses
she has been actively involved with Cribs Foundation Inc.
where she facilitates music therapy sessions for neglected children and sexually abused girls aged 7 to 17
truly found my talent and what I was good at when I was at my lowest
which is when I was experiencing mental illness
I wanted to teach other people how music can not just be a tool for entertainment
I'm currently partnering with Cribs Foundation
which is a foundation that holds neglected children and also sexually abused girls from 7 to 17 years old
truly helps them and how their eyes open up with light and joy
"And it's something that really touched my heart
and it's something that I want to share with the world."
where she achieved a spot in the Grand Finals
she also learned the art of music production that year
Dia began sharing her music on streaming services
beginning with the R&B track "Heart Hates Me." She subsequently released her debut EP titled don't quote me featuring the songs "faded," "Beautiful Disaster," "take me away," and "Dream."
followed by "I Wanna Make You Mine" in 2023
"Ganda Gandahan," became a TikTok sensation
this synth-pop anthem champions the embrace of beauty and confidence
aligning seamlessly with her pageant experiences
Dia co-wrote the song with her best friend
Dia mentioned that this release marks her reintroduction to the music scene as a singer after going through a self-discovery
"I kind of figure out where I really was [as a singer]
And I wasn't afraid anymore to be myself and make music that is out of the box
it's OPM but it's not your particular OPM like
and I accept that I'm not the best of it
I grew up in the south so I can really be conyo
He really helped me figure out who I was especially musically
and my branding as a pop girl and that's when Ganda Gandahan is born."
a few days after flying to Bolivia for the Reina Hispanoamericana 2025 pageant
Dia released her latest song "Ikaw Ang Pinili Ko."
After releasing her latest track and completing her commitments in Bolivia as Reina Hispanoamericana 2025
Dia is preparing to unveil her debut studio album this year
I'm going to release my debut album this year and your pop girl album
And I can't wait for everybody to hear it."
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Cameras in the town showed Beitar Jerusalem supporters writing “Death to Reineh’s people
To hell,” before burning the placard and allegedly attempting to set fire to a nearby corner shop
The club has never signed a player from the Palestinian community inside Israel
despite it making up around 20 percent of the country’s population
two of the top scorers in Israel's Premier League have been Palestinian citizens of Israel - Dia and Ahmed Saba
told Arab48 that he had initially thought the Beitar Jerusalem fans were rubbish collectors
“I heard noises around 5am… I looked out of my room window and saw a fire near the shops
and four unmasked young men carrying a three-metre-tall placard with writing in Hebrew saying ‘Death to Reinah’s people
He shouted at them and called the Israeli police
Reinawi said he thought that the fans probably did not come from Jerusalem itself
but were supporters of Beitar Jerusalem from a nearby Israeli town
which competes in Israel’s second division
is due to play an away match against Beitar Jerusalem on Thursday evening as part of the State Cup of Israel tournament
who play in black and yellow stripes at West Jerusalem's Teddy Stadium
is one of Israel's biggest football clubs
having won six league championships and seven cup titles in their 76-year history
are notoriously abusive towards opposing players
routinely taunting them with racist and anti-Arab chants
Fans of the club have been seen flying giant banners at their home ground depicting a man carrying a gun and emblazoned with the words "WAR!" and "La Familia"
the club's offices were torched over the signing of two Muslim football players from Chechnya - Zaur Sadayev and Dzhabrail Kadiyev
The club has also been penalised several times for incidents including booing during a moment of silence for the murdered former Israeli Prime Minister Yitzhak Rabin
singing songs deriding the Prophet Muhammad
and physically assaulting Arab maintenance workers in stadiums
The club has historic political ties to the ruling right-wing Likud Party
Israel’s culture and sports minister and a member of Likud
released footage of herself next to Beitar fans as they chanted: “May your village be burned,” “I hate all Arabs,” and “Muhammad is dead.”
technology entrepreneur Moshe Hogeg bought the club
Copyright © 2014 - 2025. Middle East Eye
Only England and Wales jurisdiction apply in all legal matters
Middle East Eye ISSN 2634-2456
a team of Israeli archaeologists has concluded after discovering a second workshop for stone tableware in northern Israel
What the archaeologists found was a man-made cave that served as both chalk quarry and workshop for making vessels from the soft stone
Its surfaces were covered with chisel marks
Inside were thousands of stone cores and other waste from making bowls
as well as tableware in various stages of production
“It is possible that large stone containers of the type mentioned in the Wedding at Cana story may have been produced locally in Galilee,” says Yardenna Alexandre, an archaeologist with the Israel Antiquities Authority who specializes in Roman-era Galilee.
Open gallery viewDr Yonatan Adler, director of excavations at a site dating to the Roman period, shows chalkstone mugs and cores uncovered two-months prior, in the village of Reina, near Nazareth, August 10, 2017.Credit: MENAHEM KAHANA/AFPPeople around the ancient world generally used pottery to eat on and to store food. The only ones to adopt stone en masse were the ancient Jews in the late Second Temple Era.
Stone is heavy, chalkstone is absorbent and it’s no walk in the park to clean. This adoption of this considerable inconvenience was apparently based on evolving thought about purity, originating with a curious omission in the Bible’s Book of Leviticus, explains Yonatan Adler of Ariel University, director of the excavations for the IAA.
In short, impurity was a metaphysical thing, not a physical one. So the absorbent properties of chalk versus glazed pottery were irrelevant to purity.
And thus Jews began to make their tableware and storage vessels out of stone in the second half of the first century B.C.E., the time of King Herod, explains Adler, adding, “We have found stone vessels throughout the country, specifically in Jewish sites.”
Given that other peoples around the world did not ascribe the imperviousness of chalkstone to impurity, workshops for stone tableware are believed to be unique to Israel.
Open gallery viewArchaeologist Yardenna Alexandre inspects chalkstone cores and mugs uncovered at an excavation site dating to the Roman period in Reineh, on August 10, 2017.Credit: MENAHEM KAHANA/AFPThe cave with the chalk workshop was found during works ahead of building a city sports center in Reineh. It is the fourth such workshop found here, the first two being near Jerusalem (one at Hizma and one at Jebal Mukabber) and the third just a kilometer from the Reineh site.
The archaeologists were surprised to find the two stone workshops, within a kilometer of one another, in the Galilee precisely because of the long-running question about the practice of Judaism in Judea versus in the Galilee, where it became influenced by Jesus, Adler says.
“Some scholars would claim Galilee Jews were different from Judean Jews with respect to Judaism, that the Judeans were more religiously observant particularly in respect to the purity laws. Some scholars would claim the Galilee Jews were more lax,” Adler says. Others figure they were about the same. “The fact that we have such large-scale production of stone vessels in Galilee for the local market really shows the strength of purity observance in Galilee at the time,” he concludes.
No, Adler says in answer to a question: It does not seem that the Galilee Jews were a bunch of skeptics who exported their stone handiwork: Stone vessels of the type they made have not been found elsewhere.
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Volume 8 - 2020 | https://doi.org/10.3389/fcell.2020.00397
Cysteine residues are reactive amino acids that can undergo several modifications driven by redox reagents
Mitochondria are the source of an abundant production of radical species
and it is surprising that such a large availability of highly reactive chemicals is compatible with viable and active organelles
we review the results highlighting the modifications of cysteines in the most abundant proteins of the outer mitochondrial membrane (OMM)
the voltage-dependent anion selective channel (VDAC) isoforms
This interesting protein family carries several cysteines exposed to the oxidative intermembrane space (IMS)
cysteine posttranslational modifications (PTMs) were precisely determined
and it was discovered that such cysteines can be subject to several oxidization degrees
ranging from the disulfide bridge to the most oxidized
The large spectra of VDAC cysteine oxidations
indicate that they have both a regulative function and a buffering capacity able to counteract excess of mitochondrial reactive oxygen species (ROS) load
The consequence of these peculiar cysteine PTMs is discussed
knowledge about the impact of irreversible Ox-PTMs on cell physiology is quite restricted
The acetylation of the N-terminal amino group also represents a protective mechanism against irreversible oxidation and seems to act as a degradation signal in the Ac/N–degron pathway
Sulfonic acid is present in mammalian cells as taurine
also an osmoregulator and participant in calcium signaling pathways
Taurine is particularly abundant in the heart
Structure of human VDAC isoforms highlighting cysteine residues and their preferred oxidation states
The structures of hVDAC2 (yellow) and hVDAC3 (light green) β-barrels were modeled using the structure of hVDAC1 [dark green (PDB ID: 2JH4)] as a template
(A) Top view of hVDACs with the N-terminal α-helix folded within the pore
(B) Side view of the β-barrels embedded in the OMM
The cysteine residues are highlighted (in red)
and their oxidative state is indicated by colored sparks: green (totally reduced)
There are no VDAC cysteines in the outer face of the membrane
Cysteine localization in the aligned sequences of human and rat VDAC isoforms
The N-terminal α-helix is shown in light orange
and Cys229 were entirely identified in the reduced form
Cys36 and Cys65 were detected in a reduced form and trioxidized to sulfonic acid
Based on MS data reported for VDAC1, VDAC2, and VDAC3 in humans and rats, we found that the oxidation state of each specific residue is the same (Saletti et al., 2018; Pittalà et al., 2020). Thus, we speculated about the relationships between location of residue and oxidation state. In Figure 1, the position of the cysteines with respect to the water/phospholipidic phases is shown. Figure 2
the oxidative modifications borne by the residues with respect to their location in structural elements of the pore
We thus noticed that cysteines whose lateral residue is embedded in the hydrophobic moiety of the membrane are trioxidized to –SO3H
since the charged modified sulfur residues protrude into the most hydrophobic part of the membrane bilayer
unless it has a function as a spot for a structure intended to dock the OMM
These residues are present only in VDAC1 and VDAC2
and these ROS are candidate to oxidize VDAC cysteines
is that types of cysteine residues may have specific roles in the operation of VDAC isoforms
the pattern of cysteine oxidation could also be the key to understanding the evolution of the three VDAC isoforms: VDAC1 has only two
one of them in sulfonic state and exposed to the hydrophobic layer of the OMM; VDAC2 has 9–11 cysteines
in the three categories we described above; thus
it has also the overoxidized one; VDAC3 has again six to seven cysteines but lacks the category of overoxidized one
whereas no evidence about the VDAC3 cysteine status or its presence within MDVs has been reported
and VDAC3 among the S-nitrosylated targets in the prostate epithelial cell line
The role of VDAC cysteine disulfide bridges or of its oxidation to sulfonic acid has yet to be discovered
It is suggested that the degree of VDAC oxidation on the mitochondrial surface could function as a signal of ROS load within the mitochondrial network
even if much more needs to be done to truly understand their biological meaning
SR conceived the review organization and wrote most of it
MP selected the references and contributed to the oxidized cysteine discovery
SF and RS reviewed and revised the text and the Mass Spectroscopy analysis
This research was partly supported by a grant from “Piano della Ricerca di Ateneo 2016–2018” of the University of Catania
to all the participants and by a MIUR PNR “Proof of Concept 2018” grant (codex: PEPSLA) to AM
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest
The authors gratefully acknowledge the Bio-Nanotech Research and Innovation Tower (BRIT; PON project financed by the Italian Ministry for Education
Cubisino (University of Catania) for modeling human VDAC structures
Hexokinase-I protection against apoptotic cell death is mediated via interaction with the voltage-dependent anion channel-1: mapping the site of binding
(Hyper)tension release by N-terminal acetylation
First things first: vital protein marks by N-terminal acetyltransferases
Chemical proteomics reveals new targets of cysteine sulfinic acid reductase
Chemical methods for mapping cysteine oxidation
Protein-thiol oxidation and cell death: regulatory role of glutaredoxins
Distinctive biochemistry in the trypanosome mitochondrial intermembrane space suggests a model for stepwise evolution of the MIA pathway for import of cysteine-rich proteins
Voltage-dependent anion channel-2 interaction with nitric oxide synthase enhances pulmonary artery endothelial cell nitric oxide production
Charged residues distribution modulates selectivity of the open state of human isoforms of the voltage dependent anion-selective channel
Enzymatic mechanisms regulating protein S-nitrosylation: implications in health and disease
oxSWATH: An integrative method for a comprehensive redox-centered analysis combined with a generic differential proteomics screening
VDAC1 cysteine residues: topology and function in channel activity and apoptosis
Mediation of the antiapoptotic activity of Bcl-xL protein upon interaction with VDAC1 protein
Identifying functional cysteine residues in the mitochondria
MIA40 is an oxidoreductase that catalyzes oxidative protein folding in mitochondria
Anamorsin is a [2Fe-2S] cluster-containing substrate of the Mia40-dependent mitochondrial protein trapping machinery
Modular biogenesis of mitochondrial respiratory complexes
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From structure to redox: the diverse functional roles of disulfides and implications in disease
Permeation of hydrophilic solutes through mitochondrial outer membranes: review on mitochondrial porins
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Mitochondrial disulfide bond formation is driven by intersubunit electron transfer in Erv1 and proofread by glutathione
ATP-dependent reduction of cysteine-sulphinic acid by S
Cloning and functional expression in yeast of two human isoforms of the outer mitochondrial membrane channel
Formation of a stabilized cysteine sulfinic acid is critical for the mitochondrial function of the parkinsonism protein DJ-1
Generator-specific targets of mitochondrial reactive oxygen species
Mitochondrial protein oxidation and degradation in response to oxidative stress and aging
MitoCarta2.0: an updated inventory of mammalian mitochondrial proteins
Mitochondrial approaches to protect against cardiac ischemia and reperfusion injury
Vascular Nox (NADPH Oxidase) compartmentalization
and endoplasmic reticulum stress response in hypertension
Canet-Avilés
The Parkinson’s disease protein DJ-1 is neuroprotective due to cysteine-sulfinic acid-driven mitochondrial localization
Genome-wide analysis of eukaryotic twin CX9C proteins
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Characterization of mammalian sulfiredoxin and its reactivation of hyperoxidized peroxiredoxin through reduction of cysteine sulfinic acid in the active site to cysteine
The mitochondrial intermembrane space: a hub for oxidative folding linked to protein biogenesis
Recombinant human voltage dependent anion selective channel isoform 3 (hVDAC3) forms pores with a very small conductance
VDAC2 inhibits BAK activation and mitochondrial apoptosis
VDAC2 enables BAX to mediate apoptosis and limit tumor development
Mitochondrial ROS regulate thermogenic energy expenditure and sulfenylation of UCP1
Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I
Cysteine oxidative post translational modifications: emerging regulation in the cardiovascular system
Distinguishing protein-coding and noncoding genes in the human genome
a channel in the outer mitochondrial membrane
H2O2-induced block of glycolysis as an active ADP-ribosylation reaction protecting cells from apoptosis
A role for cytochrome c and cytochrome c peroxidase in electron shuttling from Erv1
Protein S-Glutathionylation: a regulatory device from bacteria to humans
Characterization of SH groups in porin of bovine heart mitochondria
Porin cysteines are localized in the channel walls
Characterization of human VDAC isoforms: a peculiar function for VDAC3
Role of cysteines in mammalian VDAC isoforms’ function
Determination of the conformation of the human VDAC1 N-terminal peptide
a protein moiety essential for the functional properties of the pore
A thiol peroxidase is an H2O2 receptor and redoxtransducer in gene activation
The roles of post-translational modifications in the context of protein interaction networks
Cytoprotective effects of nitrite during in vivo ischemia-reperfusion of the heart and liver
Peroxiredoxins are conserved markers of circadian rhythms
The-N-end rule: the beginning determines the end
Kinetic and structural basis for Acyl-group selectivity and NAD (+) dependence in sirtuin-catalyzed deacylation
S-Nitrosylation: an emerging paradigm of redox signaling
Factors affecting protein thiol reactivity and specificity in peroxide reduction
Evidence for identity between the hexokinase-binding protein and the mitochondrial porin in the outer membrane of rat liver mitochondria
A classification scheme for redox-based modifications of proteins
Superoxide radical and superoxide dismutases
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Disulfide cross-linked protein represents a significant fraction of ALS-associated Cu
Zn-superoxide dismutase aggregates in spinal cords of model mice
Taking advantage of oxidation to characterize thiol-containing polymer chains by MALDI-TOF mass spectrometry
Causes and consequences of cysteine S-Glutathionylation
The hydrogen atom transfer reactivity of sulfinic acids
Mitochondrial Ccs1 contains a structural disulfide bond crucial for the import of this unconventional substrate by the disulfide relay system
A computational study of ion current modulation in hVDAC3 induced by disulfide bonds
Structural evidence that peroxiredoxin catalytic power is based on transition-state stabilization
Voltage-dependent anion channels control the release of the superoxide anion from mitochondria to cytosol
Direct cysteine sulfenylation drives activation of the Src kinase
Mitochondrial disulfide relay: redox-regulated protein import into the intermembrane space
Solution structure of the integral human membrane protein VDAC-1 in detergent micelles
Antioxidant function of thioredoxin and glutaredoxin systems
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Thiol redox control via thioredoxin and glutaredoxin systems
Monitoring of the proton electrochemical gradient in reconstituted vesicles: quantitative measurements of both transmembrane potential and intravesicular pH by ratiometric fluorescent probes
The redox environment in the mitochondrial intermembrane space is maintained separately from the cytosol and matrix
An interaction between Bcl-xL and the voltage-dependent anion channel (VDAC)promotes mitochondrial Ca2+ uptake
S-sulfhydration as a cellular redox regulation
International Human Genome Sequencing Consortium
Finishing the euchromatic sequence of the human genome
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Two enzymes in one; two yeast peroxiredoxins display oxidative stress-dependent switching from a peroxidase to a molecular chaperone function
Peroxiredoxin 1 functions as a signal peroxidase to receive
and transmit peroxide signals in mammalian cells
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VDAC-targeted drugs affecting cytoprotection and mitochondrial physiology in cerebrovascular and cardiovascular diseases
Identification of disulfide cross-linked tau dimer responsible for tau propagation
Glutathione redox potential in the mitochondrial intermembrane space is linked to the cytosol and impacts the Mia40 redox state
Kinetic control by limiting glutaredoxin amounts enables thiol oxidation in the reducing mitochondrial intermembrane space
New insights into the mechanism of permeation through large channels
Site-specific attachment of palmitate or stearate to cytoplasmic versus transmembrane cysteines is a common feature of viral spike proteins
H2S-Induced sulfhydration of the phosphatase PTP1B and its role in the endoplasmic reticulum stress response
Comprehensive identification and modified-site mapping of S-nitrosylated targets in prostate epithelial cells
Mitochondrial ion channels as oncological targets
Erv1p from Saccharomyces cerevisiae is a FAD-linked sulfhydryl oxidase
Irreversible oxidation of the active-site cysteine of peroxiredoxin to cysteine sulfonic acid for enhanced molecular chaperone activity
Bim and VDAC1 are hierarchically essential for mitochondrial ATF2 mediated cell death
and Golgi pH in single living cells with green fluorescent proteins
The redox biochemistry of protein sulfenylation and sulfinylation
The disulfide relay of the intermembrane space oxidizes the ribosomal subunit mrp10 on its transit into the mitochondrial matrix
Glutathione homeostasis and functions: potential targets for medical interventions
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Hexokinase I N-terminal based peptide prevents the VDAC1-SOD1 G93A interaction and re-establishes ALS cell viability
VDAC1 as pharmacological target in cancer and neurodegeneration: focus on its role in apoptosis
Redox regulation of mitochondrial function with emphasis on cysteine oxidation reactions
VDAC3 and Mps1 negatively regulate ciliogenesis
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Warburg revisited: regulation of mitochondrial metabolism by voltage-dependent anion channels in cancer cells
Free tubulin modulates mitochondrial membrane potential in cancer cells
Abnormal interaction of VDAC1 with amyloid beta and phosphorylated tau causes mitochondrial dysfunction in Alzheimer’s disease
Modulation of human mitochondrial voltage-dependent anion channel 2 (hVDAC-2) structural stability by cysteine-assisted barrel-lipid interactions
Influence of protein – micelle ratios and cysteine residues on the kinetic stability and unfolding rates of human mitochondrial VDAC-2
N-helix and cysteines inter-regulate human mitochondrial VDAC-2 function and biochemistry
VDAC-2: Mitochondrial outer membrane regulator masquerading as a channel
Molecular and functional characterization of VDAC2 purified from mammal spermatozoa
Influence of oxidative stress on catalytic and non-glycolytic functions of glyceraldehyde-3-phosphate dehydrogenase
Chasing cysteine oxidative modifications: proteomic tools for characterizing cysteine redox status
VDAC2-specific cellular functions and the underlying structure
Motifs of VDAC2 required for mitochondrial Bak import and tBid-induced apoptosis
Aberrant protein s-nitrosylation in neurodegenerative diseases
Essential role for the peroxiredoxin Prdx1 in erythrocyte antioxidant defence and tumour suppression
Mitochondrial outer membrane proteome of Trypanosoma brucei reveals novel factors required to maintain mitochondrial morphology
VDAC3 gating is activated by suppression of disulfide-bond formation between the N-terminal region and the bottom of the pore
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Gasotransmitter hydrogen sulfide signaling in neuronal health and disease
Individual S-acylated cysteines differentially contribute to H-Ras endomembrane trafficking and acylation/deacylation cycles
The Ca2+-dependent release of the Mia40-Induced MICU1-MICU2 dimer from MCU regulates mitochondrial Ca2+ uptake
Regulation of mitochondrial processes by protein S-nitrosylation
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Succination is Increased on Select Proteins in the Brainstem of the NADH dehydrogenase (ubiquinone) Fe-S protein 4 (Ndufs4) Knockout Mouse
Pittalà
A high resolution mass spectrometry study reveals the potential of disulfide formation in human mitochondrial voltage-dependent anion selective channel isoforms (hVDACs)
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Discovering mechanisms of signaling-mediated cysteine oxidation
Insights into the mechanism of peroxiredoxin 6 sulfenic acid reduction by ascorbate
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A lower affinity to cytosolic proteins reveals VDAC3 isoform-specific role in mitochondrial biology
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Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling
VDAC3 as a sensor of oxidative state of the intermembrane space of mitochondria: the putative role of cysteine residue modifications
Anti-cancer compounds targeted to VDAC: potential and perspectives
Swapping of the N-terminus of VDAC1 with VDAC3 restores full activity of the channel and confers anti-aging features to the cell
AIF meets the CHCHD4/Mia40-dependent mitochondrial import pathway
The VDAC2-BAK rheostat controls thymocyte survival
Protein sulfenic acid formation: From cellular damage to redox regulation
Using VAAST to identify an X-linked disorder resulting in lethality in male infants due to N-terminal acetyltransferase deficiency
H2S biosynthesis and catabolism: new insights from molecular studies
α-synuclein shows high affinity interaction with voltage-dependent anion channel
suggesting mechanisms of mitochondrial regulation and toxicity in Parkinson disease
Global analysis of protein palmitoylation in yeast
High resolution mass spectrometry characterization of the oxidation pattern of methionine and cysteine residues in rat liver mitochondria voltage-dependent anion selective channel 3 (VDAC3)
Post-translational modifications of VDAC1 and VDAC2 cysteines from rat liver mitochondria
Immotile sperm and infertility in mice lacking mitochondrial voltage-dependent anion channel type 3
The murine voltage-dependent anion channel gene family
Santo-Domingo
Modulation of the matrix redox signaling by mitochondrial Ca2+
VDAC: old protein with new roles in diabetes
Proteome analysis of mitochondrial outer membrane from Neurospora crassa
Schredelseker
High resolution structure and double electron-electron resonance of the zebrafish voltage-dependent anion channel 2 reveal an oligomeric population
Quantitative analysis of the cysteine redoxome by iodoacetyl tandem mass tags
Mitochondrial Ca2+ uptake by the voltage-dependent anion channel 2 regulates cardiac rhythmicity
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Essential role of voltage-dependent anion channel in various forms of apoptosis in mammalian cells
Shoshan-Barmatz
a multi-functional mitochondrial protein regulating cell life and death
and reactive oxygen species in hepatocellular damage
Peroxiredoxin-2 and STAT3 form a redox relay for H2O2 signaling
Reconstitution of mitochondria derived vesicle formation demonstrates selective enrichment of oxidized cargo
The redox switch: dynamic regulation of protein function by cysteine modifications
Protein oxidation: role in signalling and detection by mass spectrometry
Cysteine dioxygenase: a robust system for regulation of cellular cysteine levels
The MIA system for protein import into the mitochondrial intermembrane space
Voltage-dependent anion channel 2 modulates resting Ca2+ sparks
but not action potential-induced Ca2+ signaling in cardiac myocytes
A new pathway for mitochondrial quality control: mitochondrial-derived vesicles
Protein-thiol substitution or protein dethiolation by thiol/disulfide exchange reactions: the albumin model
Affixing N-terminal α-helix to the wall of the voltage-dependent anion channel does not prevent its voltage gating
The sulfhydryl oxidase Erv1 is a substrate of the Mia40-dependent protein translocation pathway
Reconstitution of the mia40-erv1 oxidative folding pathway for the small tim proteins
The voltage-dependent anion selective channel 1 (VDAC1) topography in the mitochondrial outer membrane as detected in intact cell
Molecular basis for redox activation of epidermal growth factor receptor kinase
The crystal structure of mouse VDAC1 at 2.3 A resolution reveals mechanistic insights into metabolite gating
Structural preferences of cysteine sulfinic acid: the sulfinate engages in multiple local interactions with the peptide backbone
AIF deficiency compromises oxidative phosphorylation
Valdez Taubas
Swf1-dependent palmitoylation of the SNARE Tlg1 prevents its ubiquitination and degradation
Vander Heiden
Bcl-xL promotes the open configuration of the voltage-dependent anion channel and metabolite passage through the outer mitochondrial membrane
Vögtle
Intermembrane space proteome of yeast mitochondria
Using intramolecular disulfide bonds in tau protein to deduce structural features of aggregation-resistant conformations
doi: 10.1146/annurev.pathol.4.110807.092314
Palmitoylated proteins: purification and identification
Protein post-translational modifications and regulation of pluripotency in human stem cells
Atp23 biogenesis reveals a chaperone-like folding activity of Mia40 in the IMS of mitochondria
Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease
Sulfonation of the resolving cysteine in human peroxiredoxin 1: a comprehensive analysis by mass spectrometry
S-Glutathionylation: from molecular mechanisms to health
Mouse VDAC isoforms expressed in yeast: channel properties and their roles in mitochondrial outer membrane permeability
having a shorter N-terminus than VDAC2 but showing the same migration in an SDS-polyacrylamide gel
is the predominant form expressed in mitochondria of various tissues
Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes
The evolutionary history of mitochondrial porins
H2S-induced sulfhydration: biological function and detection methodology
Preserving insulin secretion in diabetes by inhibiting VDAC1 overexpression and surface translocation in β cells
S-Nitrosylation in Alzheimer’s disease
Mitochondrial outer membrane voltage-dependent anion channel is involved in renal dysfunction in a spontaneously hypertensive rat carrying transfer RNA mutations
Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity
voltage-dependent anion selective channel isoforms
Foti S and Saletti R (2020) Cysteine Oxidations in Mitochondrial Membrane Proteins: The Case of VDAC Isoforms in Mammals
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what they had in mind was less a renovation than a revelation
“The place was built in 1936—one of those classic old Americana-style Route 66 motor courts,” Alison says
It’s got these beautiful old adobe brick bones
and when we pulled out part of the wall to build these new nichos—these kind of carved-in shelves—to hold the bottles for the bar
It’s really been a process of stripping things down to their essence so the adobe architecture sings and it just becomes this calm
though it’s open while the work continues)
a nicely curated selection of local food trucks will be pulling up next to the big outdoor courtyard
which operates as something of a cartoon version of a 1970s California biker clubhouse and sells t-shirts
“Steve’s an accomplished set designer,” Jay says
“and they built this place to host those types of shoots
and out of that grew this kind of clubhouse where Alison and I had our wedding reception and where we’ve had all kinds of parties
But a couple months ago we decided to do something more public.”
While both their home and their new outposts might have once seemed both far-fetched and far-flung, reality isn’t proving that to be so. “It surprised us when we moved to what we thought was the middle of nowhere that the world comes to us,” says Jay. “And that’s only increased in more recent years. People come from LA, from Austin, from New York, from everywhere. We’re more social in Joshua Tree than we’ve been in any city we’ve lived in.”
The cigar is likely most notable for its vitola, a 5 3/8 (137mm) x 48 perfecto known as the Británicas Extra that debuted back in 2012 with the Bolívar Británicas, another Edición Regional for Great Britain, and another one that showed up late as that cigar was part of the 2011 cohort. Since then, it has been used for a few other Ediciónes Regional:
Hunters & Frankau, the importer of Habanos S.A. products in the United Kingdom, commissioned 6,000 individually numbered boxes of 10 cigars, a total run of 60,000 cigars.
Of note beyond the vitola is that the band is slightly different from regular production cigars. Jemma Freeman, executive director of Hunters & Frankau, found the design while searching the brand’s archives, and while its history wasn’t immediately discernible, it does look a bit different, notably missing Cuba after Havana.
Pricing is set at £30.20 per cigar or £289 per box of 10, which works out to about $39.43 per cigar based on current exchange rates though it was closer to $37.50 when the cigar arrived in stores.
2016Courtesy of Eniac Martinez/USA Network.Save this storySaveSave this storySaveUSA’s new narco-drama Queen of the South
has perhaps one of the most bonkers backstories in TV history
which takes its inspiration from real-life players in the drug game
The novel’s first adaptation was a telenovela by the same name starring Kate del Castillo—and if the actress’s name sounds familiar
it’s because you probably heard about her earlier this year: she was the one who introduced Sean Penn to the infamous drug lord El Chapo
Vanity Fair had to speak with Queen of the South executive producer David Friendly to find out where USA’s new show fits in
Queen of the South follows a charismatic protagonist named Teresa (Alice Braga) whose desirability belies her true asset—a cunning
Cribs-worthy mansion and discusses her drug kingpin—er
she’s assassinated by a single gunshot through her window
Her death sends us plunging back into the past to find out how she got here
Friendly said when he came across La Reina del Sur three years ago
he knew he wanted to make a show out of it
which ran its own 63-episode adaptation in 2011
and was told that the English-language rights had to be acquired from the novel’s author
getting the author’s go-ahead was no cakewalk
I spoke to his agent in Spain every day begging him to let me make an offer for the English rights to the book,” Friendly said
“I thought it was a terrific story.” Eventually
his relentless perseverance won out: “They got tired of my phone calls
whose real name is Joaquín Archivaldo Guzmán Loera
runs the Sinaloa cartel in Mexico—where Queen of the South’s protagonist is from
El Chapo has escaped prison in Mexico twice; he was still a wanted convict in hiding when Penn interviewed him
How on earth did Penn manage to get in touch with El Chapo? Reina del Sur star Kate del Castillo introduced them
Turns out that she and El Chapo had been in contact ever since he reached out to her
asking del Castillo to help him make a biopic about his life
del Castillo’s involvement introduced both the actress and her telenovela to many people who had never heard of either before
As all of the intrigue surrounding his upcoming series’s real-life and fictional influences unfolded
“I was certainly aware of what was going on
he considered himself nothing more than an “interested observer.”
“It really had nothing to do with our show and what we were doing,” Friendly said
his focus remained on one thing: making his adaptation as good as it could possibly be
especially for its underserved Hispanic audience
“There is a balance going on here,” Friendly said
and making it resonate with both Hispanic and non-Hispanic viewers
required some precise considerations—and a couple tweaks to the original story line
Teresa makes her home in Spain; in USA’s series
viewers—one that also introduces the potential for some fascinating angles on ever-contentious border issues
should the show decide to go in that direction
Friendly also wanted to ensure that Spanish made its way into the show—in the right way
so obviously the main language is English,” he explained
“But there is a fair amount of Spanish in the show with subtitles.”
the show’s Brazilian leading lady Alice Braga was more than happy to put in the work that would make her character
That may be why the book’s author loved this adaptation
Braga is magnetic and confident—a believably compelling honcho in the narcosphere
That enthusiasm and energy is present both on- and offscreen; the actress was in constant communication with the directors and show-runners to make sure everything about her character
“She literally carries around a tattered copy of the book with her every shoot day,” Friendly said
Teresa Mendoza is set to return to the small screen in Season 2 of the narco series “La Reina Del Sur.” Kate del Castillo herself announced the release date and teased on a more explosive season as Teresa gets personal in her fight for survival
Hailed as one of the most successful and popular television series from Telemundo
“La Reina Del Sur” continues to depict the life of Teresa Mendoza as a woman drug trafficker in the underground world of drugs
the series recasts Kate del Castillo as Teresa
What awaits Teresa in “La Reina Del Sur” Season 2
Teresa moves out of the United States and out of the US Federal Witness Protection Program
which she was in for bringing down Mexican president Epifanio Vargas
Teresa chose to live in Italy quietly in an alias Maria Dantes and out of the radar with daughter Sofia
After eight years, the narco world found Teresa and kidnaps Sofia. The actress herself teases on Teresa’s vulnerability
and the character’s motivation to get her daughter back
Teresa will do everything to protect Sofia
even if it means reclaiming her throne again as “La Reina Del Sur.” Teresa’s search for her daughter will take viewers to a trip around the world
Del Castillo posted a teaser poster on her Instagram
captioned with the release date of the series
A post shared by instagram
Streaming giant Netflix will bring “La Reina Del Sur” Season 2 to the international scene
Telemundo has the exclusive rights to air the Columbian series in the U.S
Season 2 will premiere on April 22 at 10 pm in ET/PT
The show has reportedly ended with its most recent third season
La Reina Del Sur is streaming on Netflix globally
with all three seasons now streaming for everyone
A season 4 of La Reina Del Sur currently isn’t on the cards
The show has been big on Netflix, ultimately spending 550 unique days in various countries’ top 10s according to FlixPatrol
including strong performance in the United States
If you’re looking for a Spanish-language telenovela
there’s perhaps no better recommendation we can give than La Reina Del Sur
with 183 episodes under its belt over the course of its three seasons
The show began in 2011 but would return again to our screens in 2019 with season 2
Netflix in the United States was last to receive the most recent third season
with most of the world having already acquired the license to season 3 in December 2022
We should also note that most regions of Netflix carry the show as a Netflix Original title (although it just means it’s exclusive)
starting from season 2 the show was co-produced by Telemundo Global Studios and Netflix
All answers point to season 3 of the Spanish-language series being the last
In interviews ahead of season 3, Kate del Castillo expressed that season 3 is almost certainly the end
The actress told the Telemundo program La Mesa Caliente (translated from Spanish):
“I think the third is the last and how it should be (we must) say goodbye to Teresa
Further reporting seems to have backed this up as the final season despite official assets and promotion for season 3 not stating it’s the last. Of course, if you’ve gotten to the end of season 3, the story, as noted by numerous outlets
has concluded nicely and doesn’t leave too much room for additional stories to be told
The good news is that if you need more, Queen of the South remains on Netflix in full for the foreseeable future
All five seasons of the US-centered spinoff of La Reina Del Sur are on Netflix globally
and while it follows a similar story beat to La Reina Del Sur
there’s enough changes to keep it fresh and new
Netflix is also home to a myriad of other Telemundo shows
Other recommendations we have for you if you loved La Reina Del Sur:
Do you wish La Reina Del Sur was coming back for a fourth season on Netflix
Kasey Moore is the founder and editor-in-chief of What's on Netflix
the leading independent resource covering Netflix with over a decade of hands-on experience tracking Netflix’s new releases
His reporting and data insights have been featured in leading publications including Variety
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