Contribute Now I have been bestowed the official title of DUKE of Italy!Today, His Highness, the Prince of Cariati, Italy, Michael Chan, visited my office to bestow upon me the official title of Duke of Cariati, Italy.The Order of the Crown of Cariati is a dynastic order, a branch of the… pic.twitter.com/llxeeBisbp « Back 2023 at 12:17 am ET.css-79elbk{position:relative;}Elain Cariati (Elain Cariati campaign)MERIDEN CT — The 2023 municipal election is heating up in Meriden with plenty of races on the ballot Meriden Patch asked candidates to answer questions about their campaigns and will be publishing candidate profiles as election day draws near is running for election to the Meriden City Council as a Republican Does anyone in your family work in politics or government Now we'd like to ask a few questions about your reasons for running and your general views on politics and government I want to make a positive impact on my community There are so many issues that need to be addressed in Meriden to make it thrive: Lack of economic growth Wasteful spending on city projects and not to mention the overall quality of life Please complete this statement: The single most pressing issue facing my constituents is \_\_\_ I believe the #1 issue facing Meriden today is the lack of Leadership and accountability in the City Government Under our current administration we have seen our representatives sit silent as our emergency dispatch center for the last 2 years has been at dangerously low staffing level we have seen their budgets exceeded at the tune of over 1 Million dollars due to overtime costs Our elected representatives also oversaw and did nothing to help our citizens while the Tax Assessor manipulated property categories that helped lead to home and business owners to receive extremely high property revaluations What are the major differences between you and the other candidates seeking this post I am the only candidate putting forward concrete common-sense solutions to make Meriden a better place to live My Democratic opponents just want to continue on the same path of just throwing money at a problem in hopes it will fix itself Their way of thinking has cost our taxpayers millions downtown with nothing to show for it but vacant storefronts and blighted properties in what way has the current officeholder failed the community Meriden has gone astray for various reasons Whether it be the lack of accountability on how services are being delivered (Tax Assessors Office is a prime example) at city hall lack of transparency and communication between the city and its citizens or the disappointing narratives about our city (some coming from the current councilors) Meriden is being done a great injustice by the current administration economic development and good governance; once those things are focused on we have then started to move in the right direction Those are the beginning steps in the course correction of Meriden's current path but through collective efforts that Meriden can create a sustainable and prosperous future for residents What other issues do you intend to address during your campaign I would like to see landlords register with the city This way we have someone on file that will be responsible for any problems at the location I would also like to see City department heads (building engineering etc.) do monthly walks in areas to make a list of violations This way the city can address safety and blight issues that plague Meriden What accomplishments in your past would you cite as evidence you can handle this job The role of a city council member demands a unique set of tools and skills to effectively serve the community and passion for the community engagement form a strong foundation that I believe is essential to be an efficient and diplomatic council member I will be committed to making informed decisions and ensuring that the voice of the constituents are heard What is the best advice anyone ever gave you Is there anything else you would like voters to know about yourself and your positions I am always willing to listen to what citizens have to say I aim to help resolve and assist with any issues they may have that are within the capabilities Email vincent.salzo@patch.com for information on being featured in a candidate's profile on Meriden Patch Get more local news delivered straight to your inbox. Sign up for free Patch newsletters and alerts. passed away unexpectedly at her home on July 30 Jeanne was a teacher at Malibu Park Junior High School and a treasured member of both the Montpelier community and her longtime home in Malibu her ability to be a loving and open listener and her remarkable capacity for love touched and inspired everyone she met Jeanne’s life extended from her origins in Erie Spending their lives together from Florida and Washington to California doing what she loved most; creating poetry and stone carving Details: cache-fra-eddf8230140-FRA 1746508968 1736642731 The bride-to-be is a 2009 graduate of Pope John XXIII Regional High School and a 2013 graduate of Ohio State University She works as an operations assistant manager for American Eagle Outfitters is also a graduate of Pope John XXIII Regional High School and a 2014 graduate of Ohio State University A June 2015 wedding in Hackettstown is planned This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page Ferrari logoSearchDevelopment DriverAntonio FuocoBorn in Cariati Antonio Fuoco first raced karts at the age of four and competed in various European championships working his way up from the junior ranks to progress through to the KF2 category by 2012 when he finished third in WSK Euro Series and fourth in the CIK-FIA European KF2 Championship.He then competed in several European championships until moving up to the KF2 category he finished third in the WSK Euro Series and fourth in the CIK-FIA European KF2 Championship he made his single-seater debut competing in the newly formed Formula Renault 2.0 Alps with the Prema Junior team and was selected by the Ferrari Driver Academy The following year Fuoco debuted in European Formula 3 with Prema Powerteam finishing fifth in the standings with two wins at Silverstone and Spielberg and ten podiums in 33 races he made his debut in GP3 with Carlin Motorsport That year saw his debut in Formula 1 when he drove the Ferrari in the two days of testing following the Austrian Grand Prix finishing third in the championship and taking his first win with GP3 champion Charles Leclerc as teammate The Italian won at Monza in front of the Ferrari fans Fuoco was victorious at Monaco and Abu Dhabi with the Charouz Racing System the following year he left the FDA programme but was promoted to Scuderia Ferrari test driver while continuing to support the Academy winning the Italian Pro-Am class title with AF Corse’s 488 GT3 and Singaporean driver Sean Hudspeth he stayed with the simulator team and drove for the Scuderia at the end-of-season tests in Abu Dhabi He also competed in the Italian GT championship scoring two victories and the Italian Endurance title at the wheel of the AF Corse team’s Ferrari 488 GT3 with fellow countrymen Giorgio Roda and Alessio Rovera driving on the simulator and in a test on the Imola circuit in August He also stayed with the Ferrari Driver Academy increasing his involvement in GT racing and making his debut in the FIA WEC World Endurance Championship in the Cetilar Racing 488 GTE He then went on to win the LMGTE Am class in the 8 Hours of Portimão and had a season in the GT World Challenge Europe and Intercontinental GT Challenge at the wheel of the Iron Lynx and AF Corse 488 GT3 Evo 2020.  Fuoco raced in the FIA World Endurance Championship at the wheel of an AF Corse 488 GTE in the LMGTE Pro class finishing third with one win at the 8 Hours of Bahrain He also raced in the American IMSA series with Cetilar Racing taking a win here too in the colours of Iron Lynx while also continuing as a Development Driver for Scuderia Ferrari he also raced the Ferrari AF Corse Hypercar number 50 in WEC with three podium finishes to his name: second in the Monza 6 Hours and Portimao 6 Hours and third in the Sebring 1000 Miles He also took pole position in the 499P at the Le Mans 24 Hours.  For 2024 he continues racing in WEC in the 499P and as F1 Development Driver Cariati was pulled over after running a red light on North Colony Road on Nov She was later released on a promise to appear in court Dec Cariati did not respond to a request for comment from The Meriden Record-Journal Detection of bacteria by culturomics from catheter tips used for embryo transfer has been shown to be a reliable method to detect pathogen growth. Endometrial microbiota testing in clinical practice could certainly offer a means to further improve diagnosis and treatment strategies in IVF patients. Volume 14 - 2023 | https://doi.org/10.3389/fendo.2023.1204729 This article is part of the Research TopicReproductive Microbiome and its Interplay with the EnvironmentView all 8 articles A correction has been applied to this article in: Corrigendum: Endometrial microbiota profile in in-vitro fertilization (IVF) patients by culturomics-based analysis Introduction: It is well recognized that the human uterus and adjoining tissues of the female reproductive tract exist in a non-sterile state where dysbiosis can impact reproductive outcomes The endometrial microbiota is a part of this greater milieu it has largely been studied using 16S rRNA or metagenomics-based methodologies Despite the known advantages of sequencing analysis several difficulties have been noted including sample contamination and standardization of DNA extraction or sequencing The aim of this study was to use a culturomics-based method to analyze the endometrial microbiota and correlate the results with ongoing pregnancy rates Methods: A prospective cohort study was performed at the University of Naples from October 2022 and February 2023 Ninety-three patients undergoing an IVF cycle with single embryo transfer (ET) (fresh or frozen) were enrolled in the study the catheter tip was inserted into brain heart infusion (BHI) medium under sterile conditions for culture After 24h and 48h of incubation the microorganisms in the colonies were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) 68 (73,92%) patients resulted positive for one or more microbes and 25 patients (26,08%) had no microbial growth the four most important phyla were Firmicutes (87,76%) Actinobacteria (10,29%) and Ascomycota (8,82%) was significantly correlated with ongoing pregnancy rate (p=0,05) Staphylococcus subspecies (spp.) (p<0,05) and Enterobacteriaceae (p<0,001) were found to have a negative impact on the implantation rate Discussion: Detection of bacteria by culturomics from catheter tips used for embryo transfer has been shown to be a reliable method to detect pathogen growth Endometrial microbiota testing in clinical practice could certainly offer a means to further improve diagnosis and treatment strategies in IVF patients The primary aim of this study was to investigate the application of culturomics in clinical practice for endometrial microbiota analysis in patients undergoing IVF The secondary aim was to correlate culturomics results with IVF outcome Ninety-three infertile women aged between 29 and 47 years, undergoing the IVF technique between October 2022 and February 2023 at the IVF Unit, University of Naples Federico II, were included in the study. Specifically we included patients undergoing embryo transfer with a blastocyst classified as good quality based on morphology evaluation following the Gardner and Schoolcraft criteria (13) The exclusion criteria were: recent diagnosis of pelvic inflammatory disease (PID) clinically relevant abnormalities of the endometrial cavity including fibroids oocyte recovery failure or development of poor quality blastocytes antimicrobial treatment in the last 4 weeks A written informed consent form was signed by all participants involved in the study The embryo transfer was performed using the double-lumen catheter set in order to avoid the contamination from cervical canal the guiding (outer) catheter with a bigger diameter allow the isolation of the transfer (inner) catheter aspirated for capillarity and thanks to a syringe was added to 8mL of BHI culture medium taking great care to avoid any contact with non-sterile surfaces The samples thus obtained were sent to the laboratory for microbiological culture investigation Bacteriological analysis was performed in the laboratory of Bacteriology and Mycology at the Department of Molecular Medicine and Medical Biotechnology of the University of Naples Federico II (Figure 1) Microbial culture of the distal tip of the transfer catheter was performed to evaluate and identify the endometrial microflora at the time of ET the catheter tip was resuspended within a liquid BHI medium The samples for culture investigation were received in the laboratory within 10-15 minutes from the ET and subsequently seeded on plates of Tryptic Soy Agar (TSA) Columbia agar with colistin and nalidixic acid with 5% Sheep Blood (CNA) Agar Gardnerella Agar and Chocolate Agar in an anaerobic glove box (DW Scientific Anaerobic Workstation) The plates were incubated at 37°C for 24-48 hours in aerobic conditions except for Gardnerella Agar plates which were placed in both a 5% CO2 incubator and anaerobiosis (two plates were seeded for each sample) and Chocolate agar which was incubated under anaerobic conditions Anaerobiosis was created by using an anaerobic generator sachet (BD GasPak™) in the related jar approximately 1.5 mL of each sample was collected using a disposable Pasteur and subsequently sown on the selected media and then incubated at 37°C for 24-48 hours in aerobic about 2.5 mL of BHI were taken with the use of a sterile Pasteur and inoculated into a liquid enrichment broth Fluid Thioglycollate Medium (FTM),that supports the growth of anaerobic bacteria without the use of an anaerobic chamber Microbial growth was evaluated initially at 24 hours and subsequently at 48 hours after inoculating and diluting the previously incubated BHI medium on TSA the previously incubated FTM enrichment medium was inoculated and diluted on a solid Schaedler Agar medium and incubated at 37°C for 24-48 under anaerobic conditions as previously specified Once the incubation period in anaerobic jars was over the microbial growth of all prepared plates was evaluated Lactobacillus was cultured on Gardnerella Agar and TSA in anaerobiosis conditions after 24 and 72 hours Figure 1 Flowchart of Culturomics-based Analysis The microorganisms were identified by MALDI-TOF MS (Bruken Daltonics it permits identification of organisms by species-specific profiles of peptide and protein masses a pure colony was taken from the culture plate using a wooden stick and transferred onto a MALDI-TOF MS target plate consisting of 96 spots The spot on the target plate was then overlaid with 1-2 μL of matrix the bacterial cells were coated with formic acid/acetonitrile before adding to the matrix Fungal cells were treated with ethanol and formic acid/acetonitrile on the target plate before matrix overlay This procedure was referred to as “On Target Lysis” The matrix was applied within a short time to avoid oxidation of the sample on the target plate After a short period of drying at room temperature the plate was placed in the ion chamber of the mass spectrometer for analysis The automatically generated mass spectrum was compared to a database of mass spectra using its software resulting in the identification of the microorganism Data are presented as frequency (percentages) Comparisons between groups for categorical variables were performed with the chi-square test or with Fisher’s exact test as appropriate Continuity correction was applied where necessary the significance level was set at α = 0,05 All analyses were performed using the statistical software R Features of the patients enrolled in the study including anthropometric characteristics, lifestyle related factors, hormonal values, causes and years of infertility and numbers of previous embryo transfer was reported in Table 1. Biochemical screening, microbiological and genetic tests and strumental exams pre IVF with relative results was listed in Tables 2, 3 Table 1 Characteristics of patients included in the study Table 2 Analysis or exams of patients included in the study before undergoing to IVF Table 3 Analysis/exams pre-In vitro Fertilization techniques in both groups Table 4 Species revealed from culturomics in all patients included in the study Figure 2 Relative abundance of species between two analyzed groups Relative abundance is the percent composition of an organism of a particular kind relative to the total number of organisms in the area Following the evaluation of the bacterial isolates 47 infertile women (69,1%) were positive only for Gram-positive bacteria 15 infertile women (22%) showed positivity for both Gram-positive and Gram- negative bacteria simultaneously while the endometrium of 6 women (8,9%) had microbial colonization sustained exclusively by Gram-negative bacteria the results of the microbiological culture demonstrated that the uterine microbiota of the patients included in the study mainly consisted of bacteria belonging to the following phyla: Firmicutes (87,76%) The phylum Firmicutes was the most abundant operative taxonomic unit isolated in 59 of 68 infertile women (87,35%) the genera revealed were Lactobacillus spp (51,47%) from the cultural analysis it is evident that the greatest number of infertile women (35) had a clear prevalence of the lactobacillary population in the uterine cavity the Lactobacillus species isolated by mass spectrometry with MALDI-TOF technology were the following: Lactobacillus gasseri Lactobacillus paracasei and Lactobacillus johnsonii Taking into account patients who achieved clinical pregnancy independently from the age (35/93 27 patients (39,7%) showed endometrial bacterial colonization while only 8 patients showed no microbial development (33,3%) we did not detect significant differences between infertile women who tested positive for microbiology and those with no microbial development The microbial species present in infertile women who obtained a clinical pregnancy (Group A) were compared with the isolated species in those with an unfavorable IVF outcome (Group B) Results showed a significant difference in Lactobacillus spp. isolation in patients of Group A with respect to implantation failure (37% vs 5% p=0,05) (Table 5). In addition, the presence of the following families was associated with a poor prognosis of the IVF technique after blastocyst transfer: Staphylococcaceae (8% vs 35%, p<0,05); Enterobacteriaceae (60% vs 100%, p<0,001) (Table 5) Table 5 Comparison between Group A (pregnant patients) and Group B (no pregnant patients) However, although the uterine microbiota of the two different groups was found to be very similar in terms of taxonomic variety, the Phylum Actinobacteria including Bifidobacteriaceae, Corynebacteriaceae and Microbacteriaceae families was exclusively expressed in the group of women who did not achieve a clinical pregnancy (Group B, P=0,037) (Table 5) Specific species identified were Bacillus simplex Corynebacterium coyleae and Microbacterium maritypicum MALDI TOF analysis also revealed the presence of fungi such as Candida albicans Candida parapsilosis and Candida lusitaniae The two groups include a different identification of species of Ascomycota: Group A was positive for Candida krusei and Candida glabrata; Group B for Candida albicans Candida parapsilosis and Candida lusitanie the percentage of patients positive to the culture investigation for one or more species stands out in the foreground 74% of patients is a data that definitely open the discussion about the endometrial investigation prior to embryo transfer Significant results of this study demonstrate a positive impact of Lactobacillus spp several findings including the different expression of some families (Staphylococcaceae Enterobacteriaceae) between the two analyzed groups and the exclusively expression of the Phylum Actinobacteria in the group of women who did not achieve a clinical pregnancy demonstrate the potential negative impact of disbiotic microbiota on IVF outcome Many studies have examined the endometrial microbiome on IVF outcome. However, most of these have been performed applying a metagenomics approach (14) The use of new genomic sequencing technologies applied to the study of the uterine microbiota has limitations as the latter is considered a low biomass microbiome Culture studies of the endometrial microbiota are rare Only very few studies describe the use of culture techniques on endometrial samples and almost all are designed to reveal specific opportunistic bacteria such as Escherichia coli and Gardnerella vaginalis In this study the endometrial microbiota was analyzed by a culturomics method to test its feasibility in the clinical IVF procedure Each sample was seeded on solid and liquid media The bacterial population present in the samples was evaluated primarily by growth on solid media Enrichment broths were considered only in cases where no colonies were obtained on solid plates These results suggest that the genus Lactobacillus could be a biomarker of endometrial microflora for IVF treatment success Although further studies are needed to better understand the role of endometrial bacteria in reproductive function these preliminary results suggest an important role for microbial communities in embryo implantation and pregnancy establishment and also support the use of transfer catheters of embryos associated with scrupulous microbiological culture investigation to test the endometrial microbiota Even if culturomics provides additional information this information is only available in qualitative terms where we recall instead that sequencing is also able to provide a representation of the quantitative composition of the microbiota this study demonstrates that culture-based testing is an efficient first-level method to define microbiota in clinical practice Table 6 Comparison between NGS and culturomics A limitation of this study is the possible contamination of the microbiota population in the uterus with the vaginal microbiome Although a strategy to isolate the transfer catheter was applied as described above it is not possible to exclude completely a contamination from the cervix a eubiotic endometrial microbiota could be considered as a permissive microbial community for ongoing pregnancy regardless of the presence of minimal pathogenic bacteria the study of endometrial microbiota could be a future means of improving reproductive outcomes in infertile patients standardized protocols and larger patient cohorts are needed for studies to be comparable and help better understand uterine microbiota The raw data supporting the conclusions of this article will be made available by the authors Ethical review and approval was not required for the study on human participants in accordance with the local legislation and institutional requirements The patients/participants provided their written informed consent to participate in this study FC and CC contributed to conception and design of the study FC wrote the first draft of the manuscript RDG and AC edited and reviewed the manuscript All authors contributed to manuscript revision The author(s) declare that no financial support was received for the research The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher Future of microbiomes through the national microbiome initiative PubMed Abstract | CrossRef Full Text | Google Scholar Functional interactions between the gut microbiota and host metabolism PubMed Abstract | CrossRef Full Text | Google Scholar Introduction: microbiome in human reproduction PubMed Abstract | CrossRef Full Text | Google Scholar The endometrial microbiome and its impact on 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gastrointestinal mucosa by real-time PCR J Clin Microbiol (2002) 40(12):4423–7 Characterization of the protein subset desorbed by MALDI from whole bacterial cells PubMed Abstract | CrossRef Full Text | Google Scholar Culturing the human microbiota and culturomics Strina I and Alviggi C (2023) Endometrial microbiota profile in in-vitro fertilization (IVF) patients by culturomics-based analysis Received: 12 April 2023; Accepted: 28 June 2023;Published: 04 August 2023 Copyright © 2023 Cariati, Carotenuto, Bagnulo, Pacella, Marrone, Paolillo, Catania, Di Girolamo, Conforti, Strina and Alviggi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) distribution or reproduction in other forums is permitted provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited in accordance with accepted academic practice distribution or reproduction is permitted which does not comply with these terms *Correspondence: Federica Cariati, ZmVkZXJpY2EuY2FyaWF0aUB1bmluYS5pdA== Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher. 94% of researchers rate our articles as excellent or goodLearn more about the work of our research integrity team to safeguard the quality of each article we publish. Volume 15 - 2024 | https://doi.org/10.3389/fendo.2024.1362947 Endometrial microbiota profile in in-vitro fertilization (IVF) patients by culturomics-based analysis A Corrigendum onEndometrial microbiota profile in in-vitro fertilization (IVF) patients by culturomics-based analysis By Cariati F, Carotenuto C, Bagnulo F, Pacella D, Marrone V, Paolillo R, Catania MR, Di Girolamo R, Conforti A, Strina I and Alviggi C (2023) Front. Endocrinol. 14:1204729. doi: 10.3389/fendo.2023.1204729 the correct one was already reported in the in main body of the manuscript (Material and methods section) This sentence previously stated: “A prospective cohort study was performed at the University of Naples from June 2022 to December 2022” “A prospective cohort study was performed at the University of Naples from October 2022 and February 2023” In the published article, Figure 2 and Table 5 reported errors. In details, in Figure 2A a color label association error was made. Table 5 showed an error in data on Firmicutes/Lactobacillaceae presented as absolute value and not as a percentage. The corrected Figure 2 and Table 5 and its caption appear below In the published article, there was an error in the legend for Table 5 as published The legend “ Comparison between Group A (pregnant patients) and Group B (no pregnant patients)” does not properly match the contents Comparison between Group A (pregnant patients) and Group B (no pregnant patients) Data are presented as relative prevalence among the families of the phylum (%) *Relative prevalence with respect to the distribution of the Phylum The authors apologize for these errors and state that they does not change the scientific conclusions of the article in any way Strina I and Alviggi C (2024) Corrigendum: Endometrial microbiota profile in in-vitro fertilization (IVF) patients by culturomics-based analysis Received: 29 December 2023; Accepted: 03 January 2024;Published: 23 January 2024 Copyright © 2024 Cariati, Carotenuto, Bagnulo, Pacella, Marrone, Paolillo, Catania, Di Girolamo, Conforti, Strina and Alviggi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher 94% of researchers rate our articles as excellent or goodLearn more about the work of our research integrity team to safeguard the quality of each article we publish The Record-Journal reported that Meriden's Democratic incumbents were expected to win their respective races in the 13th Senate District Democrat Jan Hochadel faced challenges from Republican Elain Cariati and Independent Party candidate Gwen Samuel in the 13th Senate District Michael Quinn faced Republican Lamar Terrell in the 82nd House District and Democrat Jack Fazzino was challenged by Republican Joseph Vollano in the 83rd District Also, U.S. Sen. Chris Murphy, D-CT, won re-election and a third term in Congress and now we wait for the results to come in through the night and likely into Wednesday morning for several key races All eyes are on the presidential race between main party candidates Vice President Kamala Harris and former President Donald J is seeking re-election to a new six-year term and is being challenged by Matthew M Residents also voted for their local representative in the U.S and their local state representative and senators in the General Assembly in Hartford Tuesday night's vote totals will also determine whether Connecticut will allow each person to vote by absentee ballot in future elections We'll have live updates throughout the night. Check back for updates. You can follow along for live election results, and sign up for free Patch news alerts and daily newsletters the candidates are: Kamala Harris and Tim Walz Murphy is also endorsed by the Working Families Party the candidates are: Democrat Jahana Hayes and Republican George Logan Hayes is also endorsed by the Working Families Party For state senate in the 13th senatorial district the candidates are Democrat Jan Hochadel (also endorsed by the Working Families Party) Republican Elain Cariati and Independent Party candidate Gwen Samuel For state representative in the 82nd House District, the candidates are Democrat Michael Quinn (also endorsed by the Working Families Party) and Republican Lamar Terrell In the 83rd House District, the candidates are Democrat Jack Fazzino (also endorsed by the Working Families Party and Independent Party) and Republican Joseph Vollano Democrat Hilda Santiago is running unopposed Metrics details Infertility is considered a major public health issue and approximately 1 out of 6 people worldwide suffer from infertility during their reproductive lifespans genetic tests are becoming increasingly relevant in reproductive medicine More genetic tests are required to identify the cause of male and/or female infertility identify carriers of inherited diseases and plan antenatal testing genetic tests provide direction toward the most appropriate assisted reproductive techniques the use of molecular analysis in this field is still fragmented and cumbersome The aim of this review is to highlight the conditions in which a genetic evaluation (counselling and testing) plays a role in improving the reproductive outcomes of infertile couples and starting from the observation of specific signs and symptoms both partners' reproductive systems need to function in a precisely choreographed manner Our results highlight the increasing importance of molecular testing in reproductive medicine there have been a series of striking advancements in reproductive and laboratory medicine that have essentially caused these two fields to become inextricably connected Laboratory medicine now plays a critical role in all stages of the reproductive process from diagnostic approaches to the choice of the most complex therapy In particular, genetic tests are carried out for three main purposes in reproductive medicine: the identification of the infertility causes, identification of genetic diseases transmissible to offspring, and optimization of the assisted reproductive technology (ART) (Fig. 1). The three main fields of application for which genetic testing is required to improve reproductive medicine: identification of the infertility causes (a) identification of genetic diseases transmissible to offspring (b) and optimization of the assisted reproductive techniques (c) The reproductive systems of both partners function in a combined and precisely coordinated way to conceive a child; for this reason evaluation of both members of the couple is mandatory Confirmation of the clinical diagnosis through genetic evaluation (counselling and testing) can lead to more specific and targeted medical management To optimize the application of genetic tests in clinical practice we discuss (1) the genetic conditions related to infertility including the common and rare ones that are case appropriate; (2) the diagnostic strategies in families at risk of known monogenic disease transmission; and (3) the impact of PGT in the optimization of ART techniques abnormalities in sex chromosomes have a greater impact on spermatogenesis while mutations affecting autosomes are more related teratospermia or asthenozoospermia and to familial forms of obstructive azoospermia to improve and personalize the entire diagnostic–therapeutic pathway of male infertility targeted genetic tests should be performed in the presence of specific clinical pictures always after appropriate genetic counselling: (1) for diagnostic purposes (2) during clinical decision-making to establish the most appropriate ART strategy (for example in the presence of deletions of the AZFa and AZFb regions the possibility of sperm recovery using testicular biopsy is extremely low) and (3) for prognostic purposes (to establish the risk of transmitting the pathology and plan a prenatal or preimplantation diagnostic procedures) the relative information on the phenotypic aspect the laboratory tests to highlight them and the indications for antenatal genetic testing Starting from the clinical and laboratory evaluation, as shown in Tables 3, 4, 5 the main genetic conditions that could interfere with healthy reproduction are reported with the aim of improving the targeted genetic test in the presence of specific clinical pictures more details have been reported concerning the main chromosomal and genetic alterations that could interfere with healthy reproduction; for each of them the main phenotypic presentations and the laboratory tests that are available in the pre- and postnatal periods are reported The most clinically important structural disorders in infertile females are translocations both reciprocal (exchange of two terminal segments from different chromosomes) or Robertsonian (centric fusion of two acrocentric chromosomes) responsible for blocks of meiosis and structural alterations of the X chromosome Patients with reciprocal translocations are at a significantly increased risk of infertility including hypogonadotropic hypogonadism with primary or secondary amenorrhea or oligomenorrhea The balanced rearrangements do not create health problems for their carriers because they cause neither loss nor duplication of genetic information but they can give rise to gametes in which the genetic information is unbalanced and can thus become a cause of infertility or multiple miscarriage could not be clearly associated with infertility Identifying the mutations in a timely fashion is of paramount importance to managing the reproductive options and choosing a preimplantation genetic diagnosis program: the aim is to identify the specific clinical pictures in which a targeted genetic test could guide a personalized diagnostic–therapeutic treatment approach It is well known that in 20–25% of cases, perinatal mortality is caused by inherited chromosomal or genetic alterations [127] Thanks to medical awareness in recent decades preconception carrier screening has become widely requested The identification of couples at risk of transmitting a specific inherited disorder to their offspring offers the possibility of making informed reproductive choices to future parents If the reproductive partner happens to carry a gene alteration for one of the genetic conditions the pregnancy would be at risk for a child with that disease Testing is available for more than 2000 genetic disorders such as mental retardation and congenital heart disease a variety of techniques are available to identify a transmissible disorder to the offspring in the presence of carrier or affected couples Each of these techniques can be applied only during a specific time period of pregnancy or at different embryo stages in the IVF protocol The disruptive technology of NGS together with the haplotyping strategy is driving the possibility of using NIPD in clinical cases PGT has the same diagnostic motivation as the traditional PND with the advantage of advancing the timing of diagnosis at the embryo stage Only disease-free embryos are transferred to the mother Even for couples who are able to conceive naturally PGT requires the application of IVF techniques including (a) the collection of gametes from both partners; (b) the fertilization of the oocyte by intracytoplasmic sperm injection (ICSI); (c) the embryo biopsy which allows one or more cells from the blastomere or trophectoderm to be taken 3 or 5 days postfertilization; (d) molecular analysis and (e) the embryo transfer The protocol is based on an enlarged panel of disease-associated genes (approximately 5000 genes) and enables (a) the direct detection of family mutations and the indirect detection through linkage analysis of heterozygous SNPs (PGT-M); (b) a chromosomal translocation (PGT-SR) analysis; and (c) testing for aneuploidies the limitations of a single NGS protocol are related to its inability to detect haploidies the analysis of consanguineous families is not recommended other limitations regarding the limit of detection or the size of the translocation supported by the protocol could be overcome using haplotyping in the presence of the index case It was soon overcome by the analysis of the whole chromosome set by using different genetic platforms such as metaphase Comparative Genomic Hybridization (mCGH) array-based Comparative Genomic Hybridization (aCGH) single nucleotide polymorphism (SNP) microarray quantitative polymerase chain reaction (qPCR) infertility can be the result of nongenetic and genetic factors hundreds of genes must interact in a precise manner during sex determination and early development to generate healthy offspring known genetic causes of infertility include chromosomal aberrations single gene variants and phenotypes with multifactorial inheritance specific genes and mutations have been confirmed to be associated with infertility phenotypes in males and our knowledge regarding the molecular basis of infertility is continually growing Confirmation of a clinical diagnosis through genetic testing may lead to personalized medical management Similar clinical symptoms may be the result of different genetic variations genetic evaluations (counseling and testing) can contribute to the specific identification of the disease or to the confirmation of a suspected diagnosis The combination of the detailed clinical information provided and the identified genetic cause will allow the development of a personalized diagnostic–therapeutic strategy through the identification of risk factors and diseases that may be present allows the organization of targeted diagnostic–therapeutic approaches The couples in which the reproductive risk is lower are those in which an unhealthy lifestyle was evident in the absence of pathological conditions; in this case it is necessary to take action based on this information to promote a healthy lifestyle The reproductive risk increases in couples in which the presence of a disease in only one of the partners is identified there are specific interventions aimed at the patient targeted interventions are required in couples with a high reproductive risk when both partners are affected by a pathology and after the failure of all methods to achieve pregnancy naturally and the ethnicity of both groups of grandparents gynecology) should be considered to exclude maternal/paternal infertility causes In the case of identification of pathologies standard diagnostic algorithms will be used integration will need to be made with specific diagnostic procedures that require integration with eligibility criteria to access ART other molecular features of the patients should be taken into account before starting therapeutic protocols to avoid potential side effects An opportunity comes from the example of the BRCA test BRCA1 and BRCA2 are the most common genes related to hereditary breast and ovarian cancers Molecular screening for these genes has greatly increased in recent years due to technological simplification and the availability of specific drugs that are suitable for patients with mutations in these genes several affected and nonaffected young females have been found to be carriers of a BRCA mutation This information should be taken into account when reproductive choices are planned considering the potential risk of treating these patients with hormonal therapy Although it is difficult to predict at this time how much the diagnostic yield of genetic tests for the different subtypes of male and female infertility will increase it is realistic to expect a decrease in the current percentage of idiopathic infertility The general state of health in the reproductive environment is gaining increasing attention and clinical relevance reproductive specialists have the task of evaluating infertile couples by considering both their general and reproductive health since the relative conditions of comorbidity can influence their reproduction Medicine is undergoing an important transformation from a reactive to a preventive approach: the future will focus on the integrated diagnosis treatment and prevention of 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universal method for genome wide analysis of genetic disease based on mapping crossovers between parental haplotypes Live birth after PGD with confirmation by a comprehensive approach (karyomapping) for simultaneous detection of monogenic and chromosomal disorders Karyomapping-a comprehensive means of simultaneous monogenic and cytogenetic PGD: comparison with standard approaches in real time for Marfan syndrome Evaluation of targeted next-generation sequencing–based preimplantation genetic diagnosis of monogenic disease Detection and phasing of single base de novo mutations in biopsies from human in vitro fertilized embryos by advanced whole-genome sequencing Validation of next-generation sequencing for comprehensive chromosome screening of embryos ESHRE PGD consortium data collection X: cycles from January to December 2007 with pregnancy follow-up to October 2008 Clinical applications of preimplantation genetic testing Preimplantation genetic diagnosis guided by single-cell genomics Next-generation sequencing: the dawn of a new era for preimplantation genetic diagnostics 24-chromosome copy number analysis: a comparison of available technologies The impact of next-generation sequencing technology on preimplantation genetic diagnosis and screening Next-generation sequencing: challenges in reproductive genetics Novel technologies emerging for preimplantation genetic diagnosis and preimplantation genetic testing for aneuploidy Bisphenol a: an emerging threat to male fertility Recent advances in understanding & managing male infertility Frequency of the male infertility evaluation: data from the national survey of family growth Clinical relevance of genetic variants of gonadotrophins and their receptors in controlled ovarian stimulation: a systematic review and meta-analysis Recent developments in genetics and medically assisted reproduction: from research to clinical applications Download references Valeria D’Argenio & Rossella Tomaiuolo Dipartimento di Medicina Molecolare e Biotecnologie Mediche Università degli Studi di Napoli Federico II FC and RT contributed to the conception of this manuscript All authors were responsible for the literature review All authors read and approved the final manuscript The authors declare that they have no competing interests Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1186/s12967-019-2019-8 Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Menu.page-176630022{--slot3:#f0e8f6;--slot4:#b892de;--slot5:#222222;--slot6:#8254b0;--slot7:#f0e8f6;--slot8:#8254b0;--slot9:#b892de;--slot11:#222222;--slot12:#b892de;--slot14:#8254b0;--slot15:#b892de;--slot16:#222222;--textShadowColor:#b892de88;--metaColor:#8254b0;--navColor:#8254b0}Curiosity Is OKWhat Do You Call Non-Binary Or Trans Parents?Respecting a fellow parent's unique lived experiences and identity is the bare minimum As LGBTQ+ Americans of all ages continue to fight for equal rights, freedom, and safety they face discrimination at every turn just for being who they are Respecting someone’s identity should be the absolute bare minimum but for people in marginalized communities and accepted for who they are can feel like an unfortunate rarity If you’re hoping to treat the trans, non-binary, and/or gender non-conforming parents in your life with the respect and kindness they deserve, you might be wondering what, exactly, to call them. Thankfully, it’s a pretty straightforward answer with a pretty simple solution, as Rebecca Cariati, L.Ac., LGBTQ+ Health Specialist at Spectrum Chinese Medicine, tells Scary Mommy. Cariati, a non-binary parent, sums it up pretty succinctly. “There are no rules when it comes to parent titles for trans, non-binary, and gender non-conforming parents.” Within their community and with their clients, they add, “I've seen everything from ‘mom’ and ‘dad’ to sweet nicknames that are a play on the parent's first name to words in that parent's culture for a loved one,” such as “Baba” or “Renny.” “In my practice, I always ask people, ‘What does your little one call you?’ which is usually preceded by, ‘My pronouns or they/them, what are yours?’ In a social situation you could say, ‘I'm [insert your parental name], what does your kiddo call you?’” Ultimately, Cariati reassures that it really is as simple as asking someone’s name when you meet them. These introductions can also be teachable moments for your little ones, especially if they express curiosity about why someone might not go by “mom” or “dad.” Says Cariati: “The vast majority of kids are not as acculturated to binary gender guidelines as adults are. We put the gender binary overlay onto our kids, thinking pronouns, same sex couples, queer families, and ‘different’ parental names are a much bigger deal to explain than they actually are.” Hopefully, these conversations and practices will become so normalized that it will simply become the standard to respect someone’s pronouns and chosen parental identifiers. But until that time, it’s always worth a reminder that approaching any person with kindness and respect is of utmost importance. This article was originally published on Dec. 9, 2022 Storms and heavy rainfall have been affecting much of Italy over the last few days Forecasters say that there could well be further rainfall to come although things should be clamer (but colder) by Wednesday 4 December Meanwhile, Calabria, which was hit by severe flooding in late November is still suffering from the heavy rainfall roughs seas and storm surges along coastal regions Sea levels have risen to such an extent that 10 homes had to be evacuated The towns of Corigliano and Cariati have been badly affected and schools remain closed until Tuesday 3 December There have also been reports of some landslides in Crotone Sicily has seen over 100mm of rainfall in just 24 hours So far there have been only minor incidents of flooding Sources: TG Com24; Il Messaggero Italy Richard Davies is the founder of floodlist.com and reports on flooding news More Floods in Greece More Floods in Trinidad Cookies | Privacy | Contacts © Copyright 2025 FloodList Amanda Cariati's got plenty of time this time. When she plays her euphonium, Cariati can sustain her strength for 10 minutes. "Yeah," said Cariati, a San Joaquin Delta College student whose unique "baby-tuba" skills make her a center of attention Wednesday. "It gets heavy after awhile. It weighs 40 pounds. Or maybe it just feels that heavy after 10 minutes of playing." Cariati, a musical rarity who aspires to play in the American president's own band, performs an eight-minute euphonium solo - well within her limit - as a member of Delta's Symphonic Winds and Wind Ensemble on Wednesday at Atherton Auditorium. Cariati, 20, a Lincoln High School graduate, performs her solo during "Pantomime," by English composer Phillip Sparke. Flute player Baldeep Singh goes solo during Wolfgang Amadeus Mozart's Concerto for Flute in D minor. It's all part of a busy musical week that includes Delta's 11th Spring Festival of Bands on Thursday and Friday. On those days, students from 37 high schools and middle schools play 50 concerts and learn at clinics. Cariati did that during all four high-school years. She began her euphonium explorations early. "There aren't many young women who play euphonium," said Cariati, who attended Colonial Heights School and began on a "little dinky thing," a 10-pound mini-baby tuba. "I actually got interested in first grade. "I thought it was cool. I liked the sound of it. It was really beautiful. Just really warm." At Lincoln, she played in the school's marching band and became its drum major during her junior and senior years. While leading the band, she kept her euphonium skills sharp in Lincoln's concert band. She "just got back" from a Northern California Community College conference at Los Medanos College in Antioch. During two years at Delta, she's traveled to Reno as part of the All-Western Honor Band. "It's not too horribly difficult," Cariati said of mastering the euphonium. "It's like any other instrument. It takes a lot of practice." Tutored by Sacramento's Brian Schlegel, she also was influenced by Matt Tropman, a University of the Pacific instructor (tuba and euphonium) since 2010. He's already accomplished one of Cariati's goals: playing in the U.S. Marine Band (President's Own). "My immediate plan is to go into one of the military bands," Cariati said. "Since I was a kid, I wanted to be a part of the President's Own Marine Band. "Also, one of my up-in-the-clouds dreams is to go over and play with the brass bands in Europe." First, though, she's been accepted at the University of North Texas (Denton) and still is applying to the University of Washington in Seattle. "She's a very fine player," said Art Holton, who organized this week's events as director of Delta's music program and wind instruments. "The best I've ever had in my career. She's got a really nice tone quality and is really an all-round good kid and player." He described Baldeep Singh, a graduate of Cesar Chavez High School who'll play the flute solo in Mozart's Concert for Flute, with similar enthusiasm. "He's one of the most talented flutists I've had at Delta," said Holton, the former Lincoln High band director now in his 12th year at Delta. "He's very good. He really flies on the instrument. He plays well with a lot of technique." Cariati attended Delta's band festival four times as a Lincoln student. "It's been helpful in kind of rounding things off," said Cariati, one of three siblings whose single mom (Jan) plays bass trombone and works as a mechanic. "Telling me what to expect at a four-year college. I get a little taste of it before I go. It's good to play some challenging music. It's more fun than super-hard." The hands-on experience also assists in attracting and informing future students. Delta now has 70 music majors - "the most since I've been here," Holton said - and is "one of the top two or three" community college music programs in Northern California. "Education is No. 1," Holton said of Delta's design. "They get to play on a nice stage with professional judges, who are also clinicians, and work with the bands to help them get better. They provide recorded comments. "It also lets kids know what Delta is. What kind of program we have. We have students who come from 60 miles away to be in the program." That also can include the extra-curricular Community Band, whose 90 volunteer members are from Stockton and Lodi. The group performs on April 22 at the Kennedy Center in New York. "I'm absolutely ecstatic," said Cariati, who'll add her euphonium tones to the community group, which played a "send-off" concert Sunday at Atherton Auditorium. "I'm so excited." There won't be any 10-minute euphonium solos involved. Contact reporter Tony Sauro at (209) 546-8267 or tsauro@recordnet.com. Volume 11 - 2020 | https://doi.org/10.3389/fendo.2020.00453 This article is part of the Research TopicBisphenols and Male Reproductive HealthView all 10 articles Bisphenol A (BPA) is a widespread chemical agent which can exert detrimental effects on the male reproductive system Exposure to BPA has been shown to induce several epigenetic modifications in both animal and human cells BPA could not only modify the methylation pattern of multiple genes encoding proteins related to reproductive physiology but also directly influence the genes responsible for DNA methylation microscopic and macroscopic alteration of male reproductive organs and inheritable epigenetic changes involving human reproduction BPA exposure was also linked to prostate cancer This review aims to show the current scenario of BPA-induced epigenetic changes and its effects on the male reproductive system Possible strategies to counter the toxic effect of BPA were also addressed It is now acknowledged that widespread chemical agents can exert detrimental effects on human physiology. Some substances identified as “endocrine-disrupting chemicals” (EDC) could also interfere with the endocrine system (2–4) The ability of BPA to alter normal epigenetic patterns has been recently demonstrated. Some studies revealed a role in the differentiation of spermatogenic cells, through the functional modification of some genes (10, 11) This review intends to summarize the epigenetic mechanisms by which BPA acts on both animal and human male reproductive systems the possible strategies to counteract BPA effects were also disclosed and discussed Genes studied in relation to BPA exposure and male reproductive system alterations BPA is able to bind hormonal receptors, stimulating, or inhibiting the physiologic pathway. Consequently, the ability to interfere with the hormonal axis has been observed, thereby influencing steroid signaling (12). BPA affects testis competence, varying the gene expression of steroid hormone receptors and influencing the enzymes that catalyze DNA methylation, as demonstrated by in vivo and in vitro animal studies (13–21) In fishes (adult males of rare minnow Gobiocypris rarus), BPA has been demonstrated to affect the gene expression of steroid hormone biosynthesis, blood–testis barrier, proteolysis, lipid transport, and metabolism (13) The majority of the studies principally focus on molecular mechanisms of pathophysiological changes and not on proper structural abnormalities some evidence from animal studies showed how the BPA exposure promotes teratogenesis and affects testis morphology In zebrafish embryo–larvae, BPA shows teratogenic properties, provoking different anomalies going from cardiac edema to craniofacial abnormalities, spinal malformations, cranial hemorrhage, and yolk sac deformity, depending on dose of exposure (22) In mice, BPA administration appears to compromise the testis morphology; especially the size of seminiferous tubules and the epithelium were significantly reduced with impairment of spermatogenesis at various stages (21) Moreover, El Henafy et al. (16) evidenced that BPA could significantly impair anogenital distance which represents an important measure of genital development Another study showed the involvement of Sertoli cells, essential for physical and nutritional support of developing germ cells, as a target of epigenetic and transcriptome alterations from environmental toxicant exposures. These epigenetic alterations are related to testis abnormalities (23) Cho et al. studied the influence of BPA on micro-RNA (miRNA): in mouse Sertoli cell lines, the BPA was shown to alter miRNA expression, with subsequent gene expression modification, and related changes in reproductive patterns (24) An in vitro study on testicular fragments culture from 7-day-old male pigs exposed to BPA demonstrated a downregulation of EXPO5 and Dicer genes and an upregulation of Drosha and AGO2 genes, involved in miRNA pathways. Also, Leydig cells' morphology was not altered but interstitial tissue collagen was increased (25) In mice, in vitro studies on testis germ cells exposed to high doses of BPA demonstrated a decrease in the global DNA methylation levels, due to a reduction in DNMT1 protein and mRNA. At the same time, histone hypomethylation of H3K9Me3, H3K27Me, H3K9Me1, and H3K9Me2 was revealed. These changes seem to be mediated by a reduction in G9a proteins, which are essential methyltransferases for the meiotic process and hence for the whole spermatogenesis (20, 30) The toxic effect of BPA on mouse semen quality was demonstrated from Zhang et al., who observed an increased number of morphologically altered and headless spermatozoa; in addition, sperm motility was reduced, after subcutaneous injection or feeding with BPA (31). Yin et al. demonstrated the alteration of DNA methylation of MYBPH and PRKCD, eliciting a change in spermatocyte proliferation and motility in a murine model (32) In fishes, several studies showed that BPA exposure causes an impairment of global DNA methylation in the testes and consequently reduced rate of fertilization (33–36) In Danio rerio zebrafish, Lombó et al. observed sperm DNA fragmentation dependent on dose and time of BPA exposure (6) since the effects of BPA exposure are also unfolded by DNA damage and epigenetic modifications information on the influence of BPA on spermatogenesis and related male infertility is derived not only by studies directly analyzing sperm parameters but also through evidence of embryo and offspring abnormalities The process of DNA methylation is closely linked to the well-known phenomenon of genomic imprinting, wherein a gene is differentially expressed depending on whether it has been inherited from the mother or from the father. Examples of imprinting-derived diseases are Angelman syndrome and Prader-Willi syndrome. These, although caused by the epigenetic modification of the same gene, elicit different consequences depending on which parent it has been inherited from (45) When a “safe” dose of bisphenol A was administered for a long time in rats, a decrease in histone acetylation of H3K9, H3K27, and H4K12, an increase in deacetylase Sirt1 expression with reduced binding, and finally an increase in estrogen receptor β (ERβ) to caveolin-1 (Cav-1) binding were observed. These processes and the related findings provided clues about the underlying mechanisms for epigenetic inheritance induced by BPA exposure (46) Oppositely, Zhang et al. noticed no changes in methylation of IGF2, IGF2R, Peg3, and H19, which are imprinted genes. However, they acknowledged that the offspring of BPA-exposed mice had smaller size and worse pelage quality, thus admitting a certain effect of this compound (31) Shi et al. demonstrated how BPA modifies the mRNA expression of DNA and histone methyltransferases and their associated factors in the testis of a generation of mouse neonates prenatally exposed to that compound and how these effects were transmitted to the third generation of offspring (49) In Danio rerio zebrafish, treatment with BPA during embryogenesis did not impact the methylation profile of sperm, although a decrease in H3K9ac, involved in sperm development, was observed (50) An in vivo study on adult zebrafish males exposed to BPA during spermatogenesis and mated with non-exposed females revealed a disruption of cardiogenesis in forthcoming generations (51) Akhter et al. studied the appearance of different malformations in various generations of zebrafish, after that the parental generation was exposed to BPA, finding abnormalities in the testes of the second-generation males and explaining this as a trans-generational effect most probably due to epigenetic mechanisms (52) Other lines of evidence from animal studies showed that sperm motility was associated with methylation variation affecting genes involved in chromatin organization. The result of this alteration could affect embryo development (53, 54) In a study on perinatal exposure of pregnant rats to BPA, the authors observed male fertility impairments in the three subsequent generations (13) Hong et al. observed a reduction in the population of all sperm cells at different stages of development (spermatogonia, spermatocytes, and spermatids) in adult mouse testes, after exposure of preimplantation embryos to low-dose BPA, suggesting it as a consequence of epigenetic mechanisms (21) Moreover, male rats subjected to neonatal BPA exposure showed downregulation of DNMT gene expression and related transcription factors, with impact on sperm epigenome and therefore influence on embryo development and implantation process (47) In addition, after fetal exposure to BPA in utero, male rats were mated with unexposed female rats: the results showed an epigenetic alteration of IGF2 methylation in the male germline and subsequently promotion of glucose intolerance and β-cell dysfunction in the offspring, proving therefore the inheritance of epigenetic pattern changes, leading to dysregulation and disease (55, 56) Our review intended to highlight the mechanisms by which BPA modifies at various levels the reproductive system we looked into literature and summarized the studies that analyzed the epigenetic changes leading to impairment of the different aspects of male reproduction Epigenetics is responsible for the control of many genes implicated in hormonal production, sperm parameters, and inheritable abnormalities (Tables 2–6) Characteristics of the studies which analyzed hormonal axis disturbances Characteristics of the studies which analyzed morphological alterations Characteristics of the studies which analyzed the epigenetic impairment of spermatogenesis Characteristics of the studies which analyzed the transgenerational effects of BPA exposure Characteristics of the studies which analyzed the risk of prostate cancer induced by BPA exposure The interesting point that comes out from our analysis is that BPA acts on two levels of epigenetic changes it is responsible for the widely altered DNA methylation the most commonly studied epigenetic mechanism; on the other hand studies showed that the main effect of bisphenol A is on genes related to methylation proteins BPA might be considered as an example of a proper epigenetic controller In this paper, we have also illustrated the possible strategies to counteract the epigenetic effect of BPA. Indeed, several antioxidants can ameliorate reproductive function by inhibiting BPA's effect on oxidative stress (68–70) As abovementioned, flavonoids can defend from the epigenetic modifications induced by bisphenol A, due to their antioxidant and similar estrogenic properties (17). In addition, thanks to its antioxidant and free radical scavenger properties, melatonin has been demonstrated to pass the blood–testis barrier and protect steroidogenesis and spermatogenesis, acting principally on H3K9me2 and DNA methylation (20, 72) showing reduction in the hypomethylation pattern and hence pelage modification These studies support that the transgenerational effects of BPA could be reduced by folate administration In 2011, Hardy and Tollefsbol coined the term “epigenetic diet” to refer to the dietary intake of all the compounds with protective properties against epigenetic modifications, including folates, isothiocyanates, isoflavones, resveratrol, curcumin, and tea polyphenols, among others (75) data on therapeutic options to reduce the impact of BPA are still quite scarce this is the first comprehensive narrative review on BPA-induced epigenetic changes and its consequence on male reproductive health we explored the effect of BPA in any aspect of reproductive system anomalies various epigenetic targets of BPA in reproductive disorders were also analyzed we recognize that this led us also to a limitation since we did not apply a systematic approach Given the relevant epigenetic effect of BPA and other EDCs, it could be useful in future to define specific epigenetic markers associated with male reproductive dysfunction during preconceptional analysis (8, 76, 77) since epigenetic changes can be potentially treated target therapies could represent a very interesting topic of study in order to preserve fertility in subsequent generations Exposure to BPA has the potential to induce epigenetic modifications in both animal and human cells Such modifications could in turn play a role in male reproductive disorders and cancer development An epigenetic transmission to offspring was also demonstrated Further research is needed to define the mechanisms underlying BPA-related epigenetic changes in paternal sperm and offspring phenotype and to find appropriate therapies to reduce the impact of BPA-induced dysfunctions and AC contributed to the conception and design of the study FC and LC wrote the first draft of the manuscript and CA wrote the sections of the manuscript All authors contributed to the manuscript revision Bisphenol-A: epigenetic reprogramming and effects on reproduction and behavior EDC-2: the endocrine society's second scientific statement on endocrine-disrupting chemicals Air pollution and female fertility: a systematic review of literature Association between intrafollicular concentration of benzene and outcome of controlled ovarian stimulation in IVF/ICSI cycles: a pilot study The adverse health effects of bisphenol A and related toxicity mechanisms Genetic and epigenetic alterations induced by bisphenol A exposure during different periods of spermatogenesis: from spermatozoa to the progeny Bisphenol A: an endocrine disruptor with widespread exposure and multiple effects PubMed Abstract | CrossRef Full Text | Google 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to bisphenols and phthalates and association with oxidant stress Oxidative stress and alterations in DNA methylation: two sides of the same coin in reproduction Melatonin protects prepuberal testis from deleterious effects of bisphenol A or diethylhexyl phthalate by preserving H3K9 methylation Exposure of preimplantation embryos to low-dose bisphenol A impairs testes development and suppresses histone acetylation of StAR promoter to reduce production of testosterone in mice and estrogenic effects of bisphenol A and its alternative replacements bisphenol S and bisphenol AF in zebrafish embryo-larvae Epigenetic transgenerational inheritance of testis pathology and sertoli cell epimutations: generational origins of male infertility A relationship between miRNA and gene expression in the mouse sertoli cell line after exposure to bisphenol A Do G-protein coupled estrogen receptor and bisphenol A analogs influence on Leydig cell epigenetic regulation in immature boar testis ex vivo A unique 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contents DNA demethylation mediated by down-regulated TETs in the testes of rare minnow Gobiocypris rarus under bisphenol A exposure Global DNA methylation in gonads of adult zebrafish Danio rerio under bisphenol A exposure Male exposure to bisphenol a impairs spermatogenesis and triggers histone hyperacetylation in zebrafish testes H3K4me2 accompanies chromatin immaturity in human spermatozoa: an epigenetic marker for sperm quality assessment Genome-wide alteration in DNA hydroxymethylation in the sperm from bisphenol A-exposed men Association of bisphenol a exposure with line-1 hydroxymethylation in human semen LINE-1 hypomethylation in spermatozoa is associated with bisphenol A exposure DNA hydroxymethylation rate in the AChE and HoxC4 promoter associated with human sperm quality Epigenetics and imprinting in human disease PubMed Abstract | CrossRef Full Text | Google Scholar Long-term exposure to a ‘safe' dose of bisphenol A reduced protein acetylation in adult rat testes Effect of neonatal exposure on male rats to bisphenol A on the expression of DNA methylation machinery in the postimplantation embryo Aberrant DNA methylation at Igf2-H19 imprinting control region in spermatozoa upon neonatal exposure to bisphenol A and its association with post implantation loss and S induces transgenerational effects on male reproductive functions in mice Embryonic exposure to bisphenol a impairs primordial germ cell migration without jeopardizing male breeding capacity Transgenerational inheritance of heart disorders caused by paternal bisphenol A exposure Next-generation and further transgenerational effects of bisphenol A on zebrafish reproductive tissues Epigenetic analysis of high and low motile sperm populations reveals methylation variation in satellite regions within the pericentromeric position and in genes functionally related to sperm DNA organization and maintenance in Bos taurus Histone hyperacetylation and DNA methylation interplay during murine spermatogenesis Paternal BPA exposure in early life alters Igf2 epigenetic status in sperm and induces pancreatic impairment in rat offspring F0 maternal BPA exposure induced glucose intolerance of F2 generation through DNA methylation change in Gck Transgenerational actions of environmental compounds on reproductive disease and identification of epigenetic biomarkers of ancestral exposures Plastics derived endocrine disruptors (BPA DEHP and DBP) induce epigenetic transgenerational inheritance of obesity reproductive disease and sperm epimutations Developmental exposure to estradiol and bisphenol A increases susceptibility to prostate carcinogenesis and epigenetically regulates phosphodiesterase type 4 variant 4 Developmental estrogen exposures predispose to prostate carcinogenesis with aging Perinatal exposure to oestradiol and bisphenol A alters the prostate epigenome and increases susceptibility to carcinogenesis Developmental exposure to bisphenol A increases prostate cancer susceptibility in adult rats: epigenetic mode of action is implicated Neonatal exposure to estradiol/bisphenol A alters promoter methylation and expression of Nsbp1 and Hpcal1 genes and transcriptional programs of Dnmt3a/b and Mbd2/4 in the rat prostate gland throughout life DNA methylome changes by estradiol benzoate and bisphenol A links early-life environmental exposures to prostate cancer risk Prostate cancer risk and DNA methylation signatures in aging rats following developmental BPA exposure: a dose-response analysis Genome-wide analysis of low dose bisphenol-A (BPA) exposure in human prostate cells Global and region-specific post-transcriptional and post-translational modifications of bisphenol A in human prostate cancer cells Aqueous extract of Eruca Sativa protects human spermatozoa from mitochondrial failure due to bisphenol A exposure Protective action of eruca sativa leaves aqueous extracts against bisphenol a-caused in vivo testicular damages The effect of FT500 Plus(®) on ovarian stimulation in PCOS women Taurine effects on bisphenol A-induced oxidative stress in the mouse testicular mitochondria and sperm motility Melatonin ameliorates bisphenol A-induced DNA damage in the germ cells of adult male rats Pancreatic impairment and Igf2 hypermethylation induced by developmental exposure to bisphenol A can be counteracted by maternal folate supplementation Maternal nutrient supplementation counteracts bisphenol A-induced DNA hypomethylation in early development Epigenetic diet: impact on the epigenome and cancer PubMed Abstract | CrossRef Full Text | Google Scholar The evolving role of genetic tests in reproductive medicine Investigation of sperm telomere length as a potential marker of paternal genome integrity and semen quality Alviggi C and Strina I (2020) Bisphenol A-Induced Epigenetic Changes and Its Effects on the Male Reproductive System Received: 21 March 2020; Accepted: 09 June 2020; Published: 30 July 2020 Copyright © 2020 Cariati, Carbone, Conforti, Bagnulo, Peluso, Carotenuto, Buonfantino, Alviggi, Alviggi and Strina. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) *Correspondence: Federica Cariati, Y2FyaWF0aUBjZWluZ2UudW5pbmEuaXQ= †These authors have contributed equally to this work Volume 3 - 2021 | https://doi.org/10.3389/frph.2021.634813 This article is part of the Research TopicLuteal Phase Support for Assisted ReproductionView all 5 articles Luteal phase support (LPS) is crucial in assisted reproductive technology (ART) cycles when the luteal phase has been found to be defective Such deficiency is most likely related to the supraphysiological steroid levels that usually occurr in stimulated cycles which could severely affect luteinizing hormone (LH) secretion and function thereby negatively influencing the luteal phase A number of different medications and routes have been successfully used for LPS in ART Although an optimal protocol has not yet been identified the existing plethora of medications offer the opportunity to personalize LPS according to individual needs Subcutaneous administration progesterone has been proposed for LPS and could represent an alternative to a vaginal and intramuscular route The aim of the present systematic review is to summarize the evidence found in the literature concerning the application of subcutaneous progesterone in ARTs highlighting the benefits and limits of this novel strategy we carried out systematic research in the Medline and Embase databases from their inception through to November 2020 Randomized controlled trials (RCTs) were preferred by the authors in the elaboration of this article although case-control and cohort studies have also been considered in women with a good prognosis undergoing a fresh in vitro fertilization (IVF) cycle subcutaneous Pg is not inferior to vaginal products In the Frozen-thawed embryo transfer (FET) cycle data concerning efficacy is mixed with an increased miscarriage rate in women undergoing a subcutaneous route in oocyte donor recipients Data concerning the acceptance of the subcutaneous route versus the vaginal route are encouraging despite the different scales and questionnaires which were used a cost-effective analysis has not yet been conducted Recently, a water-soluble formulation of Pg for subcutaneous administration has been introduced (19, 20). A preliminary study demonstrated that this route can induce endometrial decidualization and present bioavailability similar to oil-based products (19–21) we summarize the clinical studies that have adopted subcutaneous Pg for LPS in an ART setting A systematic search was undertaken in Medline and EMBASE databases from their inception through to November 2020 studies in which subcutaneous Pg was adopted for LPS in women undergoing in vitro fertilization (IVF) or intrauterine inseminations (IUI) were selected RCTs were preferred by the authors in the elaboration of this search although case-control and cohort studies have been also considered Three authors (AC, VM, and FC) independently evaluated the risk of bias. Senior authors solved conflicts (CA and IS). Eligible RCTs were assessed using the Cochrane risk of bias assessment tool (12, 22) The following issues were assessed in detail: (1) random sequence generation; (2) allocation concealment; (3) binding of participants and personnel; (4) incomplete outcome data; and (5) selective reporting The risk of bias was graded per consideration as low Non-RCTs were assessed using the Newcastle–Ottawa scale (NOS) (23) score according to three issues: selection of study group Characteristics of studies included (fresh cycle) Characteristics of studies included (frozen cycle) A recent individual patient meta-analysis was carried out merging data from these two RCTs (34) The pooled risk difference in terms of ongoing pregnancy rates for subcutaneous Pg vs vaginal Pg was −0.03 (95% CI −0.08–0.02) the pooled risk difference regarding live birth rates was −0.02 (95% CI −0.07–0.03) the pooled odds ratio (OR) regarding OHSS risk was similar between the groups (OR 1.04 A comparison between the intramuscular and subcutaneous routes was recently carried out by Mele et al. in 2020 (30) 130 women undergoing a first IVF cycle were randomized to intramuscular Pg (33 mg/day from ovum pick up and 50 mg/day from embryo transfer) and subcutaneous Pg (25 mg/day) The authors did not observe any difference in terms of the hCGβ pregnancy test between groups what was observed was significantly higher prolactin and cortisol levels measured seven days after ovum pick up in women who had undergone intramuscular Pg compared with those who had undergone a subcutaneous route These data could suggest that the subcutaneous route might represent a solution to reduce stress and anxiety compared with the more painful intramuscular route Frozen–thawed embryo transfer is widely used in ART. In contrast with a conventional fresh cycle, the transfer of embryos did not occur immediately after OS. This approach dramatically reduced the risk of OHSS and, at the same time, offered the possibility to transfer the embryos in a more physiological environment. Recent meta-analyses suggest that the transfer of cryopreserved embryos is associated with favorable perinatal outcomes (35–38) only vitrified blastocysts reaching at least an expansion grade were transferred Both groups showed comparable body mass index Ongoing pregnancy rates (RR 1.11; 95% CI 0.78–1.56) and miscarriage rates (RR 1.08; 95% CI 0.76–1.55) were similar between groups Another retrospective trial explored the combination of both vaginal and Pg routes for LPS following FET (32) Two hundred thirteen women under 42-years-old and no history of recurrent miscarriage were included Patients were stratified according to serum Pg measured 1–2 days before embryo transfer LP was supported using vaginal formulation at a dose of 800 mg/day plus subcutaneous injections of Pg at a dose of 25 mg/day Women with Pg levels above 21.9 ng/ml showed significantly lower miscarriage rates compared with those below these cut-off values implantation and clinical pregnancy rates (gestational sac with heart activity) were similar The first one regarded previous experience with a vaginal device and the third questionnaire was 8 days later evaluating their experience with subcutaneous progesterone The authors reported better acceptance of the subcutaneous route compared with vaginal products Only one trial was developed to investigate the effect of subcutaneous Pg in women undergoing IUI (26) A total of 246 women were randomized to receive subcutaneous Pg (n = 126 Ongoing pregnancy rates per cycle (11.9 vs as was tolerability measured by a satisfactory score no cost-effective analysis has been conducted a fair amount of evidence exists to support the hypothesis that in women with a good prognosis undergoing fresh IVF cycles data concerning efficacy are mixed with one RCT conducted in oocyte donor recipients which observed reduced clinical pregnancy rates and increased miscarriage rates in women undergoing a subcutaneous route This data should be interpreted with caution considering that there is still too much uncertainty about the dosages to be used in women undergoing the FET cycle Data concerning the acceptance of a subcutaneous vs vaginal route are encouraging despite the different scales and questionnaires used to test the acceptance of women among trials subcutaneous Pg could be proposed to women who are against vaginal administration and may be associated with better tolerability and reduced injection site reaction The original contributions presented in the study are included in the article/supplementary material further inquiries can be directed to the corresponding author/s AC idealized the article and wrote the first draft All authors participated in the literature research and paper editing All authors listed have made an intellectual contribution to the work and approved the final version AC and CA decleare fees from Merck Serono Italia outside the submitted work The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest The differential secretion of FSH and LH: regulation through genes CrossRef Full Text | Google Scholar Efficacy of follicle-stimulating hormone (FSH) alone human menopausal gonadotropin or FSH + human chorionic gonadotropin on assisted reproductive technology outcomes in the “Personalized” medicine era: a meta-analysis Supplementation in ovarian stimulation for IVF: the individual PubMed Abstract | CrossRef Full Text | Google Scholar The role of recombinant LH in women with hypo-response to controlled ovarian stimulation: a systematic review and meta-analysis Daytime variation in serum progesterone during the mid-luteal phase in women undergoing in vitro fertilization treatment Luteal phase supplementation after gonadotropin-releasing hormone agonist trigger in fresh embryo transfer: the American versus European approaches Expression of vascular endothelial growth factor and its receptors in the human corpus luteum during the menstrual cycle and in early pregnancy Endometrial expression of receptivity markers subject to ovulation induction agents The luteal phase after 3 decades of IVF: what do we know PubMed Abstract | CrossRef Full Text | Google Scholar Luteal phase support for assisted reproduction cycles CrossRef Full Text | Google Scholar The efficacy of modified luteal phase support with intramuscular progesterone in IVF/ICSI cycles: a retrospective observational study Oral administration of micronized progesterone: a review and more experience PubMed Abstract | Google Scholar Progesterone use in assisted reproductive technology CrossRef Full Text | Google Scholar Dydrogesterone as an oral alternative to vaginal progesterone for IVF luteal phase support: A systematic review and individual participant data meta-analysis Luteal phase support in IVF: comparison between evidence-based medicine and real-life practices Luteal-phase support in assisted reproduction treatment: real-life practices reported worldwide by an updated website-based survey Pharmacokinetics and safety profile of a novel progesterone aqueous formulation administered by the sc route Gynecol Endocrinol Off J Int Soc Gynecol Endocrinol Randomized trial comparing the endometrial effects of daily subcutaneous administration of 25 mg and 50 mg progesterone in aqueous preparation Water soluble progesterone–hydroxypropyl-β-cyclodextrin complex for injectable formulations Cochrane Handbook for Systematic Reviews of Interventions Newcastle-Ottawa Scale: comparing reviewers' to authors' assessments Is luteal phase support successfully performed using 25 mg/day of subcutaneous progesterone in controlled ovarian stimulation cycles with GnRH agonist triggering doi: 10.26226/morressier.573c1512d462b80296c9856b Subcutaneous progesterone for endometrial preparation in substituted cycles for oocyte donation recipients: A randomized controlled trial Google Scholar Subcutaneous aqueous versus vaginal progesterone gel for luteal phase support in intrauterine insemination cycles: A pilot randomized doi: 10.26226/morressier.573c1513d462b80296c98741 Patients prefer subcutaneous progesterone over vaginal administration CrossRef Full Text | Google Scholar controlled trial comparing the efficacy and safety of aqueous subcutaneous progesterone with vaginal progesterone for luteal phase support of in vitro fertilization Subcutaneous progesterone versus vaginal progesterone gel for luteal phase support in in-vitro fertilization: a noninferiority randomized controlled study (2014) 101.doi: 10.1016/j.fertnstert.2013.09.010 In vitro fertilization and psychological stress: New insight about different routes of progesterone administration Subcutaneous versus vaginal progesterone for vitrified-warmed blastocyst transfer in artificial cycles Is there a link between plasma progesterone 1-2 days before frozen embryo transfers (FET) and ART outcomes in frozen blastocyst transfers Progesterone for preparation of the endometrium for frozen-thawed blastocyst transfer in vitro fertilization cycles: a prospective study on patients' opinions on a new subcutaneous formulation Subcutaneous progesterone is effective and safe for luteal phase support in IVF: an individual patient data meta-analysis of the phase III trials Influence of cryopreservation on perinatal outcome after blastocyst- vs cleavage-stage embryo transfer: systematic review and meta-analysis Pregnancy-related complications and perinatal outcomes resulting from transfer of cryopreserved versus fresh embryos in vitro fertilization: a meta-analysis Perinatal and obstetric outcomes in singleton pregnancies following fresh versus cryopreserved blastocyst transfer: a meta-analysis Is frozen embryo transfer better for mothers and babies Can cumulative meta-analysis provide a definitive answer? A phase III randomized controlled trial comparing the efficacy safety and tolerability of oral dydrogesterone versus micronized vaginal progesterone for luteal support in in vitro fertilization The POSEIDON criteria and its measure of success through the eyes of clinicians and embryologists Poseidon Group (Patient-Oriented Strategies Encompassing IndividualizeD Oocyte Number) A new more detailed stratification of low responders to ovarian stimulation: from a poor ovarian response to a low prognosis concept Management of women with an unexpected low ovarian response to gonadotropin Novel approaches for diagnosis and management of low prognosis patients in assisted reproductive technology: the POSEIDON concept plays a significant role for the cumulative live birth rate of a POSEIDON criteria patient Defining low prognosis patients undergoing assisted reproductive technology: POSEIDON criteria-the why Ivf during coronavirus pandemic: Who comes first The effect of FT500 Plus® on ovarian stimulation in PCOS women The Distribution of Stroma and Antral Follicles Differs between Insulin-Resistance and Hyperandrogenism-Related Polycystic Ovarian Syndrome Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome Strina I and Alviggi C (2021) Luteal Phase Support Using Subcutaneous Progesterone: A Systematic Review Received: 28 November 2020; Accepted: 25 June 2021; Published: 06 August 2021 Copyright © 2021 Conforti, Carbone, Iorio, Cariati, Bagnulo, Marrone, Strina and Alviggi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) *Correspondence: Alessandro Conforti, YWxlc3NhbmRyby5jb25mb3J0aUB1bmluYS5pdA== beloved legislator and friend of the citizens of Montpelier and beyond George Pappas; grandchildren Karis and Keller Pappas; and wife and intellect with a strong spirit and an open He will be remembered in the community for his determination to promote services and pass laws in the state legislature to benefit all people He referred to Montpelier as “My Little Town,” a favorite Paul Simon song Warren was the co-creator and owner of Onion River Sports for over 25 years Many remember his radio ads that began with “Hello Folks!” People often stopped him in the street and related their memorable experience of purchasing their first bicycle at ORS and quick wit attracted people of all ages was a member of the state legislature for 16 years including a major impact on Vermonters’ health and welfare and the revival of the Kellogg-Hubbard Library to serve the community Governor Howard Dean appointed Warren to Karen’s seat Warren’s seat mates were Francis Brooks and former mayor of Montpelier and recently retired legislator Warren retired from the Vermont State Legislature this year He served on the Commerce Committee and was an active member of Friends of the State House and the People’s Health and Welfare Committee He served on the board of Lost Nation Theater Warren was proud to coordinate the annual Karen Kitzmiller Coat Drive and by his daughters Carrie Kitzmiller and Amy Kitzmiller Pappas and sisters Kathryn Carlson and Mary Jo Yonkers who has known him since elementary school in Erie Vincent Cariati; and George’s daughter Cannon Pappas Warren kept in touch with his classmates of Academy High School in Erie and his friendship remains special to all who knew him Fritz and Jeanne reconnected in 2004 and have been married for 16 years Warren will forever be known as a star in his community believed … it is who you are in your heart that matters… A memorial service will be held at a later date Cards or notes with stories of remembrance would be warmly received at: Family of Warren Kitzmiller Do you know of something going on we should cover Nobleton resident Julia Cariati beat out thousands of other contestants to make it to the semifinals of The Greatest Baker Contest needs people to vote for her to be in first place in her grouping by Thursday to make it to the finals needs people to vote for her to win her grouping by Thursday in order to advance to the finals and the information within may be out of date When Julia Cariati applied to compete in The Greatest Baker Contest she had no idea she’d beat out thousands of other contestants and make it to the semifinals who went to Woodbridge’s Toronto District Christian High School graduated from George Brown College in Baking and Pastry Arts in 2022 She runs a baking business through Instagram called Julia’s Sweet Treats presented by the well-known “Cake Boss” Buddy Valastro will receive a package valued at about $22,500 including $10,000 (cash) and a double-page feature spread in Bake from Scratch magazine Winning the competition would bring Cariati closer to several dreams The first thing she wants to do is to donate a portion of the money to breast cancer research “My mom is currently dealing with breast cancer and I realize the only way to ensure she receives the best treatment possible is to keep donating to research,” she said in answer to competition questions “The rest of the money would be put towards fulfilling my dream of opening my own bakery,” she said “I know I am still very young and have much more to learn I would love to apply to pastry school in Europe and winning the money would make a huge difference.” The North American wide competition does not centre on one live contest pitting baker versus baker votes cast by the public will decide the winner Cariati sent in a portfolio showing highlights of what she had baked and submitted answers to competition questions Cariati said she’s loved baking for years “I have been baking for as long as I can remember I would always make desserts for family birthdays and functions I knew baking was what I wanted to do for the rest of my life.” She showed off some of her creations via her small business through Instagram (@juliassweettreats) in 2018 posting photos of cakes for friends She also began selling at local markets and pop-up shops I have grown so much and I now take custom orders all the time.” Cariati said she would bake for her brother’s birthday bring cupcakes to school and bake with her Nona friends and other followers who have helped get her to the semifinals “I didn’t think I’d make it this far” she said adding the support she has received has been “crazy” Cariati needs to be in first place in her grouping by Thursday (Jan If you’d like to vote, visit: https://greatestbaker.com/2023/julia-cariati She writes about new businesses and also highlights notable restaurants as part of yorkregion.com’s Neighborhood Eats series Simone attended Ryerson University (now TMU) for journalism She lives in Vaughan with her husband and two teenaged daughters Your browser is out of date and potentially vulnerable to security risks.We recommend switching to one of the following browsers: Invalid password or account does not exist Submitting this form below will send a message to your email with a link to change your password An email message containing instructions on how to reset your password has been sent to the email address listed on your account Selecting impeccable visual art and sculpture displays for drawing rooms and libraries can prove somewhat daunting to the untrained eye the city of Shreveport has some of the most formidable minds in the state when it comes to optioning high-end art and sculpture These are five perfectly dazzling combinations that can be procured locally Nader's Gallery features Joe Cariati hand-blown glass which is made to order in the United States  The glass works are available in shapes and styles that include decanters and lamps Each piece is entirely “free blown” — a Venetian glassblowing process that utilizes the human hand not blow molds — to form vessels from start to finish using ancient techniques that have been perfected over the course of Joe’s twenty-year career," his website states PRICE: Ranging from $600 to $2,000 and beyond The Global Views sculpture "Butterflies for Brains" complements the back splash of wall art titled “Resting Beauty" at Nader's Gallery on Kings Highway The statue is pure bronze welded together on a marble base each butterfly of the statue is connected at the wing tip There is only one small bar actually connecting the butterflies to her head PRICE: Just over $2,000 for the combination Whether selecting the perfect accessories to adorn a shelf Global Views offers some of the most fascinating and luxurious pieces around Its collection of vases was designed by Roger Thomas (designer of the Wynn hotel in Las Vegas) and are inspired by ancient and Asian influences The vases range from $172.50 for the smallest and up to $747.50 for the largest PRICE: $3,538 for the entire set of vases and the two bowls These are the sort of pieces that not only scream luxury but also tell a particular tale of the men and women who choose to display them in their homes The tall bronze people statues —  86 inches tall — are made of resin and covered with a bronze paint They are considered indoor/outdoor pieces and priced at $1,500 each Nolan Harris Simmons is a local gem who is skillfully churning out some of the most opulent and exquisite pieces of three-dimensional art the 21st century has seen Simmons was selected for exhibition at the Emerging Artist Gallery at the Bossier Arts Council in 2014 Inspired by sacred geometry and chakra meditation Simmons has two complementing pieces remaining from his Visual Vibrations series Whole Step/Half Step Diminished Scale and Harmonic Major are  on display at The Agora Borealis Both are available to area connoisseurs of fine art SHOP: The Agora Borealis, 421 Lake Street in Shreveport. Call Katy Larsen at (318) 268-3011, or contact Nolan Simmons at nolansimmons817@gmail.com. Volume 3 - 2021 | https://doi.org/10.3389/frph.2021.704153 This article is part of the Research TopicHighlights in Assisted Reproduction 2021/22View all 5 articles We report a rare case of ovarian hyperstimulation syndrome (OHSS) in a 28-year-old woman with breast cancer and with a history of polycystic ovary syndrome (PCOS) despite treatment with letrozole and gonadotropin-releasing hormone agonist (GnRH-a) triggering in a GnRH antagonist (GnRH-ant) protocol without the administration of any human chorionic gonadotropin (hCG) for luteal-phase support who underwent controlled ovarian syndrome (COS)-oocyte cryopreservation before chemotherapy Complete recovery was achieved after treatment with volume expanders it is possible to establish that estradiol (E2) modulation with letrozole and GnRH-a triggering does not eliminate the risk of OHSS it is advisable to postpone GnRH-a depot to minimize the risk of OHSS after the suspension of letrozole following menstruation or at least 7–8 days after triggering It would be desirable to identify high-risk patients in order to avoid delays in oncologic treatments that could strongly impact life expectancy We report a case of OHSS that occurred in a woman with breast cancer following COS performed in co-treatment with letrozole and triggered with GnRH-a a review of similar cases reported in the literature studies was performed All procedures were conducted in conformity with the Declaration of Helsinki and the patient signed an informed consent form there was a flap in the Douglas (26 mm x 16 mm) and there was an anterior-free fluid collection (34 × 39 mm) and white blood cell count (WBC) values were 12.8 g/dl Treatment with letrozole was suspended on the day of GnRH-a triggering and was restarted after collecting eggs until E2 levels were below 50 pg/ml Italy; 3.75 mg/28 days) was administered for ovarian protection during the chemotherapy which the patient would have started on the following day E2 and progesterone levels on the 5th day after the administration of triptorelin depot (17th after starting COS) were 195 pg/ml and 562 ng/ml the patient came to the hospital emergency room complaining of abdominal bloating USG-TV revealed markedly enlarged ovaries (70 mm × 55 mm bilaterally) and significant anterior (70 × 50 mm) and posterior (70 mm x 60 mm) free fluid the collection of a subphrenic (82 mm) and subhepatic (119 mm) fluid was found The patient was treated with volume expanders (tetramido 500 ml/day) prophylactic anticoagulation (enoxaparin sodium 4,000 UI/day) and antibiotic therapy (cefazoline 2 g/day) 20 mg of furosemide and 0.5 mg cabergoline were added to therapy the patient developed severe leukopenia (530/μl) for which filgrastrim (1/day for 7 days) was started Fluid therapy and cabergoline were continued for 12 days She was discharged on the 30th day after starting COS when Hb the size of the ovaries and the free fluid were reduced with the disappearance of the collection of subhepatic and subphrenic fluid Her chemotherapy regimen was scheduled for the same day the patient restarted letrozole administration and received triptorelin depot 3.75 mg The OHSS occurred on the 6th day after the depot Several cases of different grade of OHSS have been reported following treatment with GnRH-ant associated with GnRH-a triggering in non-oncologic patients (7–14) These cases raise questions about the dogma that the presence of exogenous or endogenous hCG is required for the onset of OHSS Possible explanations for these observations include variations of genes such as allelic variants of hCGs and their receptors The dosage of androgen levels in the days preceding the administration of GnRH-a depot could be useful in the decision-making process the onset of OHSS followed the use of GnRH-a depot Cases of OHSS reported after GnRH agonist depot The waiting duration before safely administering GnRH-a depot after PU is not well established but the authors assumed that waiting 7–8 days before the administration may be sufficient to avoid an OHSS However, it is interesting to note that, in the case reported by Friedler et al., OHSS occurred after the administration of a GnRH-a, which is used as luteal phase support (LPS) (8) Regarding the LPS, it is important to introduce the concept of “luteal costing,” which could be adapted to patients with cancer (29, 30) The idea of individualizing the LPS by monitoring serum progesterone levels could be used in these patients to identify the best timing for the administration of GnRH-a depot to prevent the onset of OHSS the Notch pathway seems to affect the expression of VEGFR3 through regulating VEGFR3 promoters it is feasible to hypothesize that the Notch pathway is dysregulated in PCOS Future research studies are needed to establish a clinically useful connection between the receptor mutations and the occurrence and intensity of OHSS there is a higher risk profile linked to increased vascular permeability and angiogenic stimulus it is possible to suggest that [1) OHSS is still possible even after modulating estrogen production with letrozole and using GnRH-ant and that the use of GnRH-a triggering is to be preferred due to the necessity to prevent comorbidities and delays in adjuvant therapy and [2) it is advisable to postpone GnRH-a depot for minimizing the risk for OHSS an ideal timing has to be identified (i.e. or at least 7–8 days after triggering) it would be desirable to identify high-risk patients Written informed consent was obtained from the individual(s) for the publication of any potentially identifiable images or data included in this article GI and CA contributed to the conception and design of the study GI and MR wrote the first draft of the manuscript LC and AC wrote sections of the manuscript and CA did the final editing of the manuscript All authors contributed to the review of the manuscript The reviewer HF declared a past co-authorship with one of the authors AL to the handling 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Marca A and Alviggi C (2021) Severe Ovarian Hyperstimulation Syndrome in a Woman With Breast Cancer Under Letrozole Triggered With GnRH Agonist: A Case Report and Review of the Literature Received: 01 May 2021; Accepted: 10 June 2021; Published: 06 July 2021 Copyright © 2021 Iorio, Rovetto, Conforti, Carbone, Vallone, Cariati, Bagnulo, Di Girolamo, La Marca and Alviggi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) *Correspondence: Giuseppe Gabriele Iorio, Z2l1c2VwcGVnLmlvcmlvOTRAZ21haWwuY29t; orcid.org/0000-0002-1723-3420 2021 at 1:27 pm ET.css-79elbk{position:relative;}Meriden election results CT — The votes have all been counted in Meriden and here are the totals for the office of mayor The vote totals are courtesy of the Secretary of State website and some candidates ran on multiple party lines which is why they are listed more than once EGG HARBOR TOWNSHIP — Pope John’s Emily Carrollo has one high school performance left The whole state can finally take a deep breath when she’s done Carrollo won her second consecutive Non Public A 400 and 800-meter titles Saturday afternoon at Egg Harbor Township What makes this win so special is that the races were about 45 minutes apart Rain and thunderstorms on Friday afternoon moved the 800 who also is deeply involved with her club soccer team this time of year perhaps the most physically demanding of all the races The rest of the top five in the 800 were fresh and did not run in the 400 “After Friday we knew we had an outside shot at winning the team title so I gave her the option of running just the 800 or both,” Pope John coach Brian Corcoran said Carrollo will run the 800 and the 4x400 — along with Kelsey Steele Ashley Taylor and Sarah Bieganousky — at the Meet of Champions Who knows how many seconds faster Carrollo could have run in the 800 if she hadn’t raced 45 minutes earlier but Corcoran still thinks her time will put her in the fast heat of the 800 where she was third in the state last season depending on who enters (many of those runners are also terrific 1,600 runners) should be the most exciting of the event The Pope John girls finished fourth in Non Public A with 50 points Paramus Catholic and Immaculate Heart tied for first with 120 points and Notre Dame (58 points) was third will make her second consecutive trip to the Meet of Champions as she was second in the 400 hurdles in 1:08.81 Senior Sarah Cariati was fifth in the 1,600 (5:05.95) but was just under three seconds from the winner It was Cariati’s best time this season and could approach the five-minute mark in the Meet of Champions Cariati had only 45 minutes or so between and 800 and 1,600 Senior Sylvia Okoye threw her best of the season in the shot put finishing fifth (35-1.50) and onto the Meet of Champions on Wednesday She also set a personal record in the discus Senior Melissa Romero was second in the javelin at 125 feet “She was a little disappointed in her performance last year and maybe got a little too caught up in watching (two-time champion) Shannon Sullivan She wanted to show everyone maybe last year was a fluke for her.” won at 133 feet and is the favorite for the state championship Pope John’s Kathleen Oroho was fourth 116-11 and also qualifies for her second Meet of Champions and should do much better than her 37th place she had last year The Pope John boys finished third in Non Public A with 66 points Don Bosco Prep won the championship with 98 points and Seton Hall Prep at second with 66 After running a season-best 11.08 in the preliminaries junior Jon Hilgart placed third in the 100 meters in 11.29 Sophomore Dylan Hurley ran a season-best 49.30 in the 400 placing third in a field that included Parros and state 400 freshman record holder Najee Glass of St Kyle Nelson and Greg Moore was second to Seton Hall in 3:23.11 Senior Ricky Okoye again will qualify for three Meet of Champions events He won the discus (which was over two days because of the weather) in 153-9 three inches better than Seton Hall’s Chris DiLorenzo Okoye was second in the triple jump (43-04) and was fifth in the long jump (19-11.25) He’s likely to do the triple jump and discus on Wednesday Sophomore Cole Ferrand was second in the shot put in 51-3.50 Pole vaulters Christian Lavorgna and Sean Reid also will be in the Meet of Champions The Vernon boys and girls did not have an individual qualify for the Meet of Champions nor did either score in the team events 15th in the triple jump and 20th in the javelin His high jump (6-2) was tied with eight other competitors It is possible he could be a wild card entrant in the Meet of Champions junior Allie Kleine was 16th in the long jump (15-9.75) with teammate Mallory Costello placing right behind her (17th Costello was also 18th in the triple jump (33-1) Haley Merritt was 21st in the 800 meters (2:28.47) and sophomore Megan Kelly was 18th in the 3,200 in 12 minutes Freshman Meg Kleeschulte was 22nd in the 3,200 in 12:06.78 Veritas Christian pole vaulters Josh and Jon Brinkerhoff qualified for the Meet of Champions for the second straight year with Jon winning with 14 feet and Josh placing second in 13-6 They are likely to both be in the top eight in the state on Wednesday.