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Il segreto di Pulcinella è un’espressione che indica qualcosa che in realtà non è più confidenziale questo idiomatismo assume un altro significato: è il nome della pizzeria di Giuseppe Bove Giuseppe Bove ripercorre il percorso paterno frequentando l’istituto alberghiero di Benevento e facendo esperienze lavorative in diverse cucine Nel 2012 apre un ristorante a Santa Maria a Vico (Caserta) ma paga l’inesperienza in campo imprenditoriale Da questa delusione nasce poi la prima pizzeria Il Segreto di Pulcinella a Montesarchio (paese di residenza dell’allora fidanzata Antonella il cui nome rimanda all’avventura conclusasi poco felicemente e al dispiacere celato agli occhi altrui Giuseppe impara il mestiere del pizzaiolo da autodidatta Ricerca ingredienti di qualità sempre crescente con l’andare del tempo e con l’aumentare della clientela il 17 maggio 2023 apre il nuovo Il Segreto di Pulcinella nell’attuale sede di Via Napoli due forni a legna nella cucina a vista (più un terzo usato per asciugare e rigenerare) numerose referenze vinicole in esposizione Il Segreto di Pulcinella ha uno stile lineare e confortevole La mise en place è semplice e curata; i piatti delle pizze riportano il logo del locale Tra il personale di sala c’è anche Antonella La pizzeria dispone di un ampio parcheggio esterno pizze al padellino e pizze tonde di scuola napoletana L’impasto delle pizze – leggero e digeribile – è di sapore piuttosto neutro; in molti casi è il topping ad essere protagonista: realizzato con ingredienti di estrema ricerca spesso esclusivi (ad esempio lo speck d’oca affumicato lavorati e abbinati con la sapienza tipica di uno chef Per alcune ricette di montanare e padellini vengono impiegate farine di tipo 1 o ai 7 semi Gli oli extravergini sono di un’ottima azienda beneventana Assai ricercati anche i drink da fine pasto In occasione di una cena riservata alla stampa organizzata dalla giornalista Laura Gambacorta abbiamo potuto degustare una selezione di pizze come amano dire qui) realizzato con l’impasto del padellino PanPizza: Impasto tripla lievitazione in doppia cottura (in forno con cottura al vapore e poi tostata sempre nel forno) Nella versione vegetariana la tartare è sostituita da funghi porcini spadellati PanPizza: Impasto tripla lievitazione in doppia cottura (in forno con cottura al vapore e poi tostata sempre nel forno) Signature Pizza (dall’amore per i cardellini di Giuseppe) ‘O cardill: Montanara in doppia cottura (fritta e ripassata al forno) con impasto ai 7 semi Interessantissimo l’uso delle verdure amare mentre l’impasto ai semi regala un’ottima masticabilità ‘O cardill: Montanara in doppia cottura (fritta e ripassata al forno) con impasto ai 7 semi Ricetta della tradizione con ingredienti selezionati la Provola e Pepe: Ristretto di pomodoro biologico Provola e Pepe: Ristretto di pomodoro biologico Creativa la Capasanta Flowers: Vellutata di topinambur Capasanta Flowers: Vellutata di topinambur Buonissima La Norma secondo Bove: Pomodoro arrosto concentrato con melanzane concassé crumble di basilico e olio extravergine di oliva Al posto della classica foglia di basilico sulla sommità viene posto un crumble preparato con farina di mandorle e basilico fresco La Norma secondo Bove: Pomodoro arrosto concentrato con melanzane concassé Mette tutti d’accordo Ortolana Bove: Crema di verdure selvatiche Gran Murgiano (formaggio vegetale al latte di mandorla) basilico e olio extravergine di oliva Seleca Una delle pizze ortolane più buone che mi sia capitato di assaggiare Ortolana Bove: Crema di verdure selvatiche Può fungere anche da dessert la Bufala Cheese che prevede sei formaggi di bufala: in cottura mozzarella di bufala e moringhello di bufala; dopo la cottura I formaggi sulla superficie vengono poi fiammeggiati con il cannello per dare loro un po’ di calore e consentire al tutto di amalgamarsi meglio Non una classica “Quattro formaggi” dunque ma una “Sei formaggi” della stessa lattifera Bufala Cheese – In cottura: mozzarella di bufala moringhello di bufala Dopo la cottura: Camembert di bufala Il Segreto di Pulcinella è una di quelle mete capaci di ispirare al viaggio: non fatevi scoraggiare dalla distanza prendetela come una sfida per raggiungere un premio ambito Il Segreto di PulcinellaVia Napoli, 92 – Montesarchio, Beneventotel. 329/8760545www.facebook.com/IlSegretodiPulcinellaMontesarchioAperto solo a cena.Giorno di chiusura: martedì Tag: Il tuo indirizzo email non sarà pubblicato email e sito web in questo browser per la prossima volta che commento Δdocument.getElementById( "ak_js_1" ).setAttribute( "value" Questo sito utilizza Akismet per ridurre lo spam. Scopri come vengono elaborati i dati derivati dai commenti Prossimi appuntamenti EVENTI GOLOSI DEL MESE – Maggio 2025 Gelaterie / News FENICE DI CONOSCERLO, A CASERTA UN PERCORSO DEGUSTAZIONE PER IMPARARE A CONOSCERE IL GELATO DI QUALITÀ Ristoranti TAVERNA LA RIGGIOLA EVENTI GOLOSI DEL MESE – Aprile 2025 News ITINERARIO SQUISITO: UN VIAGGIO SENSORIALE ATTRAVERSO LA CITTÀ DI NAPOLI EVENTI GOLOSI DEL MESE – Marzo 2025 News / Pasticcerie BIAGIO MARTINELLI PASTICCERIA, NUOVA SEDE ATTIVA DALLA COLAZIONE AL DOPOCENA EVENTI GOLOSI DEL MESE – Febbraio 2025 Pizzerie PIZZERIA DA MIMÌ AD AVERSA EVENTI GOLOSI DEL MESE – Gennaio 2025 Prodotti e produttori DA ASSAGGIATRICE AMATORIALE AD ASSAGGIATRICE ONAF CASEIFICIO SAVOIA Scuole di cucina L’ABC DELLA PASTICCERIA A CITTÀ DEL GUSTO Pasticcerie GOLOSITÀ CAMPANE Viaggi ALL’ASSAGGIO DI… PARIGI Eventi e resoconti FESTA A VICO 2012 All'assaggio BUON COMPLEANNO ALL’ASSAGGIO! Botteghe QUALCOSA DI TÈ PIZZERIA SALVO A SAN GIORGIO A CREMANO ALL’ASSAGGIO DI… NORIMBERGA Questo sito non rappresenta una testata giornalistica in quanto viene aggiornato senza alcuna periodicità non può considerarsi un prodotto editoriale ai sensi della legge n° 62 del 7/03/2001 — A new olive oil company in Tampa is connecting the Caudine Valley of Italy with the beaches of Florida They credit their success to a hard-working family and one specific type of olive grew up picking olives in their hometown of Montesarchio Mauriello said no Costiera Extra Virgin Olive Oil bottle is quite the same The ceramic jars are all made from scratch in Italy and shipped to the United States “This premium olive oil deserves a premium packaging,” said Mauriello but it’s the best packaging for this special olive oil.” You could say he was born into the business so we produce a high-quality premium olive oil from just one kind of olive called Peranzana,” said Mauriello The couple always had aspirations beyond Italy “We came down here like five or six times with my wife and the kids The small family business officially expanded to a second continent The response from Tampa Bay restaurants and stores has been better than they could have ever hoped He said the best part about sharing his family's olive oil with the people of Tampa Bay is the look on their faces when they try it they are used to buying olive oil in the supermarkets For more information on Costiera Extra Virgin Olive Oil and how to buy it, go to www.costieragroup.com Report a typo The video released on 6 August showed a person light a match and place it on the grass before running away near Montesarchio a town 31 miles (49km) from Naples in Italy In Italy the civil protection authority warned on Monday of more fires to come as temperatures in parts of the country reach 45C Electrochimica ActaCitation Excerpt :Acid-cleavable hydrazine N-succinimidyl-4-(2-pyridyldithio) pentanoate (SPP) and N-succinimidyl-4-(2-pyridyldithio) butyrate (SPDB) are some examples of cytosolic cleavable linkers [44] Several modifications that hinder the renal filtration are represented by attachments of PEG [45] It is conceivable to use automated analytical instrumentation to measure binding constants such as High-Performance Liquid Chromatography (HPLC) a strategy dependent on the partition of bound and unbound aptamer between the stationary and mobile phase capillary electrophoresis (CE) based on differential migration performed inside capillaries when a potential is applied International Journal of Biological MacromoleculesCitation Excerpt :To achieve this an organic compound capable of recognizing these G-quadruplexes in the cytoplasm is required Thanks to the enthusiastic efforts of scientists the research of organic compounds capable of recognizing G-quadruplexes has made great progress in recent years [17–21] Compounds such as N-TASQ and QUMA-1 were developed for in situ detection of intracellular RNA G-quadruplexes [22–24] Metrics details Looking for new metal-based anticancer treatments in recent years many ruthenium complexes have been proposed as effective and safe potential drugs In this context we have recently developed a novel approach for the in vivo delivery of Ru(III) complexes preparing stable ruthenium-based nucleolipidic nanoaggregates endowed with significant antiproliferative activity Herein we describe the cellular response to our ruthenium-containing formulations in selected models of human breast cancer By in vitro bioscreens in the context of preclinical studies we have focused on their ability to inhibit breast cancer cell proliferation by the activation of the intrinsic apoptotic pathway possibly via mitochondrial perturbations involving Bcl-2 family members and predisposing to programmed cell death the most efficient ruthenium-containing cationic nanoaggregates we have hitherto developed are able to elicit both extrinsic and intrinsic apoptosis To limit chemoresistance and counteract uncontrolled proliferation multiple cell death pathways activation by metal-based chemotherapeutics is a challenging yet very promising strategy for targeted therapy development in aggressive cancer diseases such as triple-negative breast cancer with limited treatment options original knowledge on ruthenium-based candidate drugs and new insights for future optimized cancer treatment protocols The investigated Ru(III) nucleolipidic complexes (a) ToThyRu and (b) DoHuRu along with the low molecular weight Ru(III) complex (c) AziRu we have herein investigated in-depth the anticancer profile and mode of action of Ru(III)-containing liposomes by focusing on well-established breast cancer cell lines as in vitro models for human mammary tumours we here describe via preclinical bioscreens how nucleolipidic ruthenium complexes are able to activate specific molecular cell death pathways thereby interfering with cancer cells growth and proliferation We also provide evidence that could strengthen novel drug-discovery strategies and ultimately lead to more selective ruthenium-based anticancer agents evaluated by the MTT assay and monitoring of live/dead cell ratio for MCF-7 and CG-5 cell lines following 48 h of incubation with the indicated concentration (the range 1 → 1000 μM has been explored and of the ruthenium-containing (15% mol/mol) POPC formulations (ToThyRu/POPC and DoHuRu/POPC) and DOTAP formulations (ToThyRu/DOTAP and DoHuRu/DOTAP) Data are expressed as percentage of untreated control cells and are reported as mean of five independent experiments ± SEM (n = 30) (b) Representative microphotographs at a 200 × magnification (20 × objective and a 10 × eyepiece) by phase-contrast light microscopy of MCF-7 (left panels) and MDA-MB-231 (right panels) breast cancer cells treated for 48 and 72 h with ruthenium IC50 micromolar concentrations of DoHuRu/POPC (18.9 and 14.7 μM respectively) and DoHuRu/DOTAP liposomes (10.3 and 12.1 μM showing the cellular morphological changes and the cytotoxic effects on cell monolayers The shown images are representative of three independent experiments (c) Representative microphotographs of untreated (left panel) and 48 h DoHuRu/DOTAP treated (right panel) MCF-7 cells by phase-contrast light microscopy at a 600 × magnification (30 × objective and a 20 × eyepiece) DoHuRu/DOTAP (at IC50 concentration) induces the formation of autophagic vacuoles detectable in cell cytoplasm Inset: higher magnifications of MCF-7 cells before and after treatment autophagic vacuoles clearly appear when MCF-7 cells were treated with the DoHuRu/DOTAP liposomes for 48 h at IC50 concentration providing a morphological support of autophagy activation These observations were further confirmed by FACS analysis the AziRu in vitro treatment by the DoHuRu/POPC liposome seems to trigger exclusively apoptosis whereas the one with the cationic DoHuRu/DOTAP seems to simultaneously activate apoptosis and autophagy Apoptosis was evaluated by FACS analysis after cell labeling with propidium iodide (PI) and FITC-Annexin V MCF-7 (a) and MDA-MB-231 (b) cells were both unlabeled and untreated (CTR−) treated with DoHuRu/POPC or with DoHuRu/DOTAP for 48 and 72 h using IC50 concentrations The lower left quadrants of each panels show the viable cells which exclude PI and are negative for FITC-Annexin V binding The upper left quadrants contain the non-viable negative for FITC-Annexin V binding and positive for PI uptake The lower right quadrants represent the cells in early apoptosis that are FITC-Annexin V positive and PI negative The upper right quadrants represent the cells in late apoptosis positive for both FITC-Annexin V binding and for PI uptake The experiments were performed at least three times with similar results early and late apoptotic MCF-7 (c) and MDA-MB-231 (d) cells after 48 and 72 h of treatments are shown Data are expressed as percentage of untreated control cells and are reported as mean of four independent experiments ± SEM (n = 24); ***p < 0.001 vs (e) DNA fragmentation assay on MCF-7 and MDA-MB-321 cells treated or not (C) with IC50 concentrations of DoHuRu/POPC (RuPOPC) and DoHuRu/DOTAP (RuDOTAP) for 48 h respectively) of cisplatin (cDDP) as the positive control for DNA fragmentation the DNA was extracted and visualized on 1.5% agarose gel as detailed in the Methods section The lane in the middle corresponds to the molecular weight markers The agarose gel is representative of three independent experiments Western blot analysis showing the effects of IC50 concentrations of DoHuRu/POPC and DoHuRu/DOTAP following 48 and 72 h of incubations in MCF-7 (a) and MDA-MB-231 (b) cells on caspases-3 Shown are blots representative of four independent experiments the bands resulting from MCF-7 (c,e) and MDA-MB-231 (d,f) cell extracts were quantified by densitometric analysis and plotted in line (solid and dotted line for Bax and Bcl-2 proteins -8 and -9) graphs as percentage of control in relation to the used ruthenium-containing nanoaggregate Shown are the average ± SEM values of four independent experiments The anti-tubulin antibody was used to standardize the amounts of proteins in each lane In the same way, ruthenium treatment in MDA-MB-231 breast cancer cells via DoHuRu/POPC and DoHuRu/DOTAP in vitro administration at IC50 concentrations elicited caspase-9 activation after 48 h, and this anew was coupled to the simultaneous Bax and Bcl-2 up-regulation and down-regulation, respectively (Fig. 4b,d,f) in this breast cancer cell line DoHuRu/DOTAP seemed able to promote full length pro-caspase-8 cleavage as evidenced by the formation of the active p10 and p18 fragments activated initiator caspases further process other caspase members which generally leads to complete the programmed cell death process the immunoblotting analysis performed on MDA-MB-231 cells exposed to Ru(III)-containing liposomes further revealed a marked proteolytic cleavage of the inactive proenzyme to activate caspase-3 the evaluation of apoptosis regulatory proteins in breast cancer models for the in vitro preclinical evaluation of ruthenium biological effects suggests the invariable induction of the mitochondrial apoptotic cell death pathway but also the cell-specific activation of the extrinsic death pathway particularly in the case of the cationic nanoaggregate Quantitative flow cytometric analysis of autophagosomes formation (MDC incorporation) in MCF-7 (a) and MDA-MD-231 (b) breast cancer cells treated with IC50 concentrations of DoHuRu/POPC or with DoHuRu/DOTAP for 48 and 72 h The main fluorescence intensities (MFIs) were calculated values are expressed as percentage of control cells and are reported as mean of four independent experiments ± SEM (n = 24); ***p < 0.001 vs it cannot be excluded that the simultaneous activation of different mechanisms of cell death can be caused by multiple potential interactions at the subcellular/molecular level the activation of multiple death pathways by metal-based chemotherapeutics in aggressive cancer diseases with limited treatment options is a largely desired goal in order to possibly restrict the onset of chemoresistance as well as to efficiently counteract uncontrolled proliferation Upcoming investigations on the critical proteins and pathways involved in autophagy control exerted by Ru(III)-containing liposomes are underway to strengthen knowledge in favour of future in vivo applications for these high potential candidate drugs Human HaCaT keratinocytes and rat L6 skeletal muscle cells were grown in the same experimental conditions Cell viability was evaluated using the MTT assay procedure which measures the level of mitochondrial dehydrogenase activity using the yellow 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT The assay is based on the redox ability of living mitochondria to convert dissolved MTT into insoluble purple formazan the medium was removed and the cells were incubated with 20 μl/well of a MTT solution (5 mg/mL) for 1 h in a humidified 5% CO2 incubator at 37 °C The incubation was stopped by removing the MTT solution and by adding 100 μl/well of DMSO to solubilize the obtained formazan the absorbance was monitored at 550 nm using a microplate reader (iMark microplate reader Cell number was determined by TC10 automated cell counter (Bio-Rad providing an accurate and reproducible total count of cells and a live/dead ratio in one step by a specific dye (trypan blue) exclusion assay Bio-Rad’s TC10 automated cell counter uses disposable slides TC10 trypan blue dye (0.4% trypan blue dye w/v in 0.81% sodium chloride and 0.06% potassium phosphate dibasic solution) and a CCD camera to count cells based on the analyses of captured images Once the loaded slide is inserted into the slide port the TC10 automatically focuses on the cells detects the presence of trypan blue dye and provides the count trypan blue can enter the cell allowing living cells to be counted after treatments in 96-microwell culture plates the medium was removed and the cells were collected mixed with 0.4% trypan blue solution at 1:1 ratio were loaded into the chambers of disposable slides The results are expressed in terms of total cell count (number of cells per ml) the instrument also accounts for the dilution and shows live cell count and percent viability Total counts and live/dead ratio from random samples for each cell line were subjected to comparisons with manual hemocytometers in control experiments The calculation of the concentration required to inhibit the net increase in the cell number and viability by 50% (IC50) is based on plots of data (n = 6 for each experiment) and repeated five times (total n = 30) IC50 values were obtained by means of a concentration response curve by nonlinear regression using a curve fitting program and are expressed as mean values ± SEM (n = 30) of five independent experiments Human breast cancer cell lines were grown on 60 mm culture dishes by plating 5 × 105 cells cells were incubated for 48 h with IC50 concentrations of the ruthenium-containing liposomes (DoHuRu/POPC and DoHuRu/DOTAP) under the same in vitro experimental conditions described above and were then morphologically examined by a phase-contrast microscope (Labovert microscope Leizt) for autophagic vacuoles and apoptotic markers Microphotographs at a 200 × total magnification (20 × objective and 10 × eyepiece) were taken with a standard VCR camera (Nikon) cells were collected and the pellets were suspended in lysis buffer (50 mM Tris-HCl 0.5 mg/mL Proteinase K) and incubated at 50 °C 10 mg/mL RNase was added to the lysates and incubated for 1 h at 50 °C DNA was precipitated with NaOAc pH 5.2 and ice cold 100% EtOH and centrifuged at 14000 × g for 10 min Pellets were dissolved in TE buffer (10 mM Tris-HCl A 20 μL aliquot of each DNA sample was analyzed on a 1.5% agarose gel stained with ethidium bromide and visualized under UV light The study was carried out by triplicate determination on at least three separate experiments Treated cells were incubated with 50 μM MDC in PBS at 37 °C for 15 min and immediately analyzed by flow cytometry with a FACScalibur flow cytometer (Becton Dickinson) The fluorescent emissions were collected through a 530 nm band pass filter (FL1 channel) At least 104 events were acquired in log mode CellQuest software (Becton Dickinson) was used to calculate mean fluorescence intensities (MFIs) The MFIs were calculated by the formula (MFI treated/MFI control) where MFI treated is the fluorescence intensity of cells treated with the various compounds and MFI control is the fluorescence intensity of untreated and unstained cells Values reported in the figures are the mean values ± SEM from three independent experiments The supernatant fraction was obtained by centrifugation at 15,000 × g for 10 min at 4 °C and then stored at −80 °C Protein concentration was determined by the Bio-Rad protein assay (Bio-Rad Normalization of results was ensured by incubating the nitrocellulose membranes in parallel with the tubulin antibody (Sigma-Aldrich) All data were presented as mean values ± SEM The statistical analysis was performed using Graph-Pad Prism (Graph-Pad software Inc. CA) and ANOVA test for multiple comparisons was performed followed by Bonferroni’s test Antiproliferative effects of ruthenium-based nucleolipidic nanoaggregates in human models of breast cancer in vitro: insights into their mode of action Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Metal-based anticancer chemotherapeutic agents Platinum salts in advanced breast cancer: a systematic review and meta-analysis of randomized clinical trials Cisplatin in cancer therapy: molecular mechanisms of action Novel metal-based anticancer drugs: a new challenge in drug delivery Approaching tumour therapy beyond platinum drugs: status of the art and perspectives of ruthenium drug candidates Recent developments in ruthenium anticancer drugs A ruthenium anticancer compound interacts with histones and impacts differently on epigenetic and death pathways compared to cisplatin Editorial of Special Issue Ruthenium Complex: The Expanding Chemistry of the Ruthenium Complexes Appraisal of the redox behaviour of the antimetastatic ruthenium(III) complex [ImH][RuCl(4)(DMSO)(Im)] Transport of therapeutic vanadium and ruthenium complexes by blood plasma components Fighting Cancer with Transition Metal Complexes: From Naked DNA to Protein and Chromatin Targeting Strategies a new redox-active anticancer agent–preclinical development and results of a clinical phase I study in tumor patients Molecular mode of action of NKP-1339 - a clinically investigated ruthenium-based drug - involves ER- and ROS-related effects in colon carcinoma cell lines A theoretical study on the hydrolysis process of the antimetastatic ruthenium(III) complex NAMI-A Anticancer cationic ruthenium nanovectors: from rational molecular design to cellular uptake and bioactivity Ruthenium anticancer compounds: myths and realities of the emerging metal-based drugs Thirty years of the drug candidate NAMI-A and the myths in the field of ruthenium anticancer compounds: a personal perspective Cholesterol-based nucleolipid-ruthenium complex stabilized by lipid aggregates for antineoplastic therapy Pyridine analogues of the antimetastatic Ru(III) complex NAMI-A targeting non-covalent interactions with albumin Ru(III) complexes for anticancer therapy: the importance of being nucleolipidic Ruthenium-based complex nanocarriers for cancer therapy A new design for nucleolipid-based Ru(III) complexes as anticancer agents Cationic liposomes as efficient nanocarriers for the drug delivery of an anticancer cholesterol-based ruthenium complex Structure-activity relationships for NAMI-A-type complexes (HL)[trans-RuCl4L(S-dmso)ruthenate(III)] (L = imidazole Apoptosis-inducing activity of cisplatin (cDDP) against human hepatoma and oral squamous cell carcinoma cell lines Discoveries and controversies in BCL-2 protein-mediated apoptosis Cancer drug resistance: an evolving paradigm Relevance of breast cancer cell lines as models for breast tumours: an update Choosing the right cell line for breast cancer research Platinum resistance in breast and ovarian cancer cell lines Insights into the in vitro Anticancer Effects of Diruthenium-1 Interaction of anticancer Ru(III) complexes with single stranded and duplex DNA model systems Insights into the mechanism of action and cellular targets of ruthenium complexes from NMR spectroscopy Ruthenium(II) Complexes as Potential Apoptosis Inducers in Chemotherapy doi: 10.2174/1871520616666160622085441 (2016) Broad targeting of resistance to apoptosis in cancer Molecular mechanisms for tumour resistance to chemotherapy Mitochondrial apoptosis: killing cancer using the enemy within Down-regulation of caspase 3 in breast cancer: a possible mechanism for chemoresistance Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation Major apoptotic mechanisms and genes involved in apoptosis Extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy Cytoxicity and apoptotic mechanism of ruthenium(II) amino acid complexes in sarcoma-180 tumor cells Thirty years of BCL-2: translating cell death discoveries into novel cancer therapies Impaired BAX protein expression in breast cancer: mutational analysis of the BAX and the p53 gene Targeting BCL-2 to enhance vulnerability to therapy in estrogen receptor-positive breast cancer The induction of mitochondria-mediated apoptosis in cancer cells by ruthenium(II) asymmetric complexes Caspase-mediated crosstalk between autophagy and apoptosis: Mutual adjustment or matter of dominance Targeting autophagy in cancer management - strategies and developments Simultaneous Induction of Non-Canonical Autophagy and Apoptosis in Cancer Cells by ROS-Dependent ERK and JNK Activation Regulation of the autophagic bcl-2/beclin 1 interaction Nucleolipid nanovectors as molecular carriers for potential applications in drug delivery Pharmacological inhibition of HSP90 and ras activity as a new strategy in the treatment of HNSCC Aurora B expression in post-puberal testicular germ cell tumours Antagonistic effects of chloroquine on autophagy occurrence potentiate the anticancer effects of everolimus on renal cancer cells Cysteine Prevents the Reduction in Keratin Synthesis Induced by Iron Deficiency in Human Keratinocytes 2,3,7,8-Tetrachlorodibenzo-p-dioxin promotes BHV-1 infection in mammalian cells by interfering with iron homeostasis regulation Deregulation of HOX B13 expression in urinary bladder cancer progression Regulatory role of rpL3 in cell response to nucleolar stress induced by Act D in tumor cells lacking functional p53 Palmitoylethanolamide inhibits rMCP-5 expression by regulating MITF activation in rat chronic granulomatous inflammation Download references This work was supported by the Italian Association for Cancer Research (AIRC) (IG n We also thank MIUR (PRIN 2010 – BJ23MN_007) for financial support Carlo Irace and Gabriella Misso: These authors contributed equally to this work CSGI - Consorzio Sistemi a Grande Interfase conceived and designed the experiments and analyzed data prepared the manuscript and assembled figures in vitro bioscreens and immunoblotting assays conducted light microscopy experiments and DNA fragmentation assay prepared and characterized POPC and DOTAP ruthenium-containing nanoaggregates All authors discussed the results and commented on the manuscript The authors declare no competing financial interests Download citation Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing newsletter — what matters in science analytics and third-party cookies.By continuing to browse The Consulate General of Italy in Munich and the Italian Cultural Institute present the most important archaeological exhibition on the ancient Samnite people ever held curated by the Staatliche Antikensammlungen / State Collections of Classical Antiquities aims to provide a broad overview of the history Thanks to the rich loans especially from the collections of Benevento (the Arcos Museum and the Samnium Museum) Montesarchio (the Samnium Caudino Archaeological Museum) and Campobasso (the Samnite Museum  Casino Calvitti di Larino) but also of Alfedena the Samnite culture can be appreciated in a complete exhibition for the first time outside Italy the Samnites came to the fore in history due to the rivalry with Rome which with alternating events (including the famous episode of the “Caudine Forks”) ended up incorporating them into its sphere of influence The exhibition highlights the distinctive features of a warlike people who managed to preserve their cultural independence for a long time despite the growing influence of the Romans The exhibits on display also testify the belonging of Samnite culture’s belonging to the Hellenistic koiné of Greek influence developed in central Italy starting from the classical period (5th and 4th centuries BC) and common to the Romans themselves and to other Italic peoples such as the Etruscans More information at iicmonaco.esteri.it Archeo3D'Italia is the video-platform dedicated to the Italian archaeological sites registered on the UNESCO World Heritage List The Rovereto International Festival of Archaeological Film was founded in 1990 with the aim of reaching and raising awareness among.. Italy has created many methodological experiences and reflections The Unification of Italy made it necessary to harmonize the different laws on the protection of archaeological and artistic heritage... The section ‘Let's talk about archaeology’ edited by Andrea Balletta e Gianluca Biscardi is a road route spanning 30,000 kilometers and.. Thanks for visiting The use of software that blocks ads hinders our ability to serve you the content you came here to enjoy We ask that you consider turning off your ad blocker so we can deliver you the best experience possible while you are here The situation in Italy is dire when it comes to COVID-19 currently with more than 1,000 deaths.  Authorities and science are doing the impossible to make progress in combating this virus Tocilizumab is a drug that treats moderate to severe rheumatoid arthritis It can also treat polyarticular juvenile idiopathic arthritis (PJIA) and systemic juvenile idiopathic arthritis (SJIA) Now its being tried on COVID-19 and with some encouraging results Italy has been experimenting with this drug to fight Covid-19  This news was spread in the Italian press and from the sources of Italian secure communications: RAI-TV A collaboration between the Colli Hospital the Cancer Institute of Naples and Chinese doctors it was verified that “Tocilizumab” can give positive results in the care of people infected with Coronavirus Two patients from Naples immediately responded  The drug was administered to two patients hospitalized at Cotugno Hospital (in Naples) suffering from severe Covid-19 pneumonia last Saturday who arrived at the hospital as a particularly critical case This was reported by the Neapolitan hospital of the Hills The same drug has been used in China in 21 patients and now for the first time has been administered in Italy in Coronavirus patience Based on this experiment it is currently evaluated if treating other critically ill patients is recommended The result was made possible thanks to a great team effort mentioning: the Oncology of the Azienda dei Colli by Vincenzo Montesarchio the Oncological Immunotherapy and Innovative Therapies of Pascale by Paolo Ascierto together with the virologist Franco Buonaguro including Wei Haiming Ming from the First Affiliated Hospital of University of Science and Technology of China as well as the team of doctors from Cotugno director of the department of infectious diseases and infectious diseases director of Uoc Diseases respiratory infectives director of the Uoc Anesthesia resuscitation and intensive care director of the Uoc Systemic infections and immunosuppressed head of the Infectivology First Aid always by Cotugno and Luigi Atripaldi director of the Microbiology laboratory and virology Paolo Ascierto and Vincenzo Montesarchio explained out how 21 patients had been treated All of them showed a visible improvement already in the first 24-48 hours after treatment Treatment included a single solution It acted without interfering with the protocol therapeutic use based on antiviral drugs Germany this news may be good also for Roche Pharma the manufacturer of the medicine.  4 injections sell for 1900,00€ © 2022 St Kitts Nevis Observer / Nevis Printing Ltd Newspaper Theme by tagDiv | All rights reserved Feature Films Database Southern Mediterranean films database Scriptwriters European Film Schools Production Companies Distributors International Sales Submit a Film Industry Reports Co-Production Podcast Online Screenwriting Training Course Guided Course for Feature Film Writing Script Analysis Analysis of the potential of your series Cineuropa's Training Catalogue Film Festival Photographs Newsletter Photogalleries EUFCN Location Award Euro Film Fest 27 Times Cinema GoCritic! Advertise on Cineuropa Logos and Banners FILMS / REVIEWS Italy by Camillo De Marco 02/07/2024 - Documentary filmmaker Romano Montesarchio makes his fiction debut feverish and hallucinating angle to a story of mafia and denied love Only a cinema encyclopaedia can tell us how much the psychopathology of criminal behaviour in mafia systems has fascinated screenwriters and directors, both in a grotesque and a dramatic register. Romano Montesarchio, an expert documentarian, adopts instead an anti-realistic and personal angle for his fiction debut, Glory Hole which world premiered in the Panorama section of the Shanghai International Film Festival the filmmaker from Caserta draws inspiration from very concrete and cruel news (and political) items this phenomenon of eco-mafias that have indiscriminately poisoned parts of the Italian territory corrupting and enriching themselves without measures at the expense of citizens isn’t to direct another episode of Gomorra but to dive into the internal dimension of a protagonist of organised crime a white collar criminal of the Camorra organisation who makes money with the illegal disposal of toxic waste The plot of Glory Hole isn’t one to make you jump out of your seat with its originality It’s a love story in a criminal environment distorted and sick to the point of paradox in which the very negation of love and beauty is required written by the director with Edgardo Pistone and Stefano Russo reserves a few interesting drifts and shifts that take us through the 95-minute running time Silvestro (a new demonstration of skill by Francesco Di Leva) meets the young Alba (Mariacarla Casillo) daughter of the boss for whom he works (Gaetano Di Vaio an impossible union that goes against all the rules of mafia families and which soon spirals into chaos Silvestro is forced to hide in one of those bunkers full of tunnels used by fugitives the ones we see in news reports – tombs equipped with all the comforts except for freedom of movement (well done to the set designer Massimiliano Forlenza) Silvestro is helped by two childhood friends a priest (Mario Pirrello) who seems lost as well and the extravagant owner of a private club to which we owe the film’s title (Roberto De Francesco But the circle is getting tighter for Silvestro paranoia keeps rising and reality turns into a nightmare With a very current aesthetic that looks to the younger spectator who grew up with Nicolas Winding Refn the photography by Matteo Vieille Rivara knows no half measures playing with the in and out of focus and colouring each scene with very clear tones grey-blue natural light or the blood red neon of the club or the green of the subterranean tunnels in which polarised human figures move The indecipherable strings orchestrated by maestro Mario Tronco and a directing consisting of fixed cameras and slow movements interspersed with images from surveillance cameras create an obsessive and claustrophobic film “I hate pretending to be happy”) in its search for the humanity in the monster the film reveals itself slowly and culminates in a final monologue of brutal concreteness a confession-J’accuse very close to a civil theatre performance: “I’ve been the solution to all your problems… I’ve gained power and money in exchange for the health of my children’s children.” Glory Hole was produced by Bronx Film, Minerva Pictures, Eskimo, Rai Cinema. Minerva Pictures Group handles international sales Please subscribe to our newsletter to receive more stories like this directly in your inbox The veteran Italian director’s movie, which will close the Venice Film Festival, is set during the Liberation and is led by Filippo Scotti   25/07/2024 | Production | Funding | Italy Documentary filmmaker Romano Montesarchio makes his fiction debut, and manages to lend a personal, feverish and hallucinating angle to a story of mafia and denied love   02/07/2024 | Films | Reviews | Italy 06/05/2025Crossing Europe 2025 Review: Slackers 06/05/2025Crossing Europe 2025 Review: Callas, Darling 02/05/2025goEast 2025 Review: My Magical World 30/04/2025Films / Reviews – Italy Review: San Damiano 30/04/2025Hot Docs 2025 Review: King Matt the First 29/04/2025Films / Reviews – Italy Review: Storia di una notte Subscribe to our newsletter to receive the most important daily or weekly news on European cinema Festivals / Awards Spain The 21st Play-Doc continues the dialogue between past and present Crossing Europe 2025 Crossing Europe 2025 Cannes 2025 Marché du Film The Party’s Over! leads France TV distribution’s Cannes slate CPH:DOX 2025 CPH:DOX Industry Europa Distribution explores the release of documentaries at CPH:DOX Cannes 2025 Marché du Film AFCI runs its second annual Global Film Commission Network Summit at Marché du Film Festivals / Awards Czech Republic Czech Republic’s Anifilm goes sci-fi Distribution / Releases / Exhibitors Europe European Arthouse Cinema Day set to return on 23 November Cannes 2025 Marché du Film Indie Sales presents a three-star line-up at Cannes HOFF 2025 The Shadow and U Are the Universe win at Estonia’s Haapsalu Horror and Fantasy Film Festival Crossing Europe 2025 Awards The New Year That Never Came and The Flats crowned at Crossing Europe Cannes 2025 Marché du Film Be For Films to sell Love Me Tender in Cannes Market TrendsFOCUSA busy spring festival season awaits the European film industry. Cineuropa will continue to keep its readers up to date with the latest news and market insights, covering the buzziest events, including Cannes, Kraków, Karlovy Vary, Tribeca, Hot Docs, Annecy, Brussels, Munich and many others Distribution, Exhibition and Streaming – 06/05/2025Europa Distribution explores the release of documentaries at CPH:DOXThe network has held a case study workshop as part of its brand-new partnership with the Copenhagen-based festival Distribution, Exhibition and Streaming – 02/05/2025Slovak crime-thriller Černák becomes the highest-grossing film in domestic cinemasThe second film in the saga about a local mafia boss, directed by Jakub Króner, outgrossed its first part, which dominated Slovak cinemas last year Jaśmina Wójcik • Director of King Matt the First The Polish director discusses her approach to taking on a 1920s children’s literary classic in an unexpected way Želimir Žilnik • Director of Eighty Plus The Serbian director discusses his deep suspicion of ideologies in relation to his irresistibly charming latest feature, which follows a man whose life spans three political systems Paulina Jaroszewicz • Distribution and marketing manager, New Horizons Association Cineuropa sat down with the Polish distributor to discuss her company’s strategy as well as the connection between its distribution line-up and BNP Paribas New Horizons Festival’s programme Lorcan Finnegan • Director of The Surfer The Irish filmmaker discusses his mystery-thriller, how he created the character with Nicolas Cage and his approach to the use of colours in the film Privacy Policy The images used on this website have been provided by journalists and are believed to be free of rights if you are the owner of an image used on this website and believe that its use infringes on your copyright We will remove the image in question as soon as possible We have made reasonable efforts to ensure that all images used on this website are used legally and in accordance with copyright laws About us | Contact us | Logos and Banners Mission | Partners | Team | Donations | Terms and conditions Italian cruise line Costa Crociere has been fined €1m for the Concordia cruise ship sinking which killed 32 people. Costa had asked for a plea bargain deal to respond to the administrative sanctions which under Italian law are for companies whose employees commit crimes Judge Valeria Montesarchio of the Tuscan tribunal accepted the plea after a hearing has sought to blame the disaster entirely on captain Francesco Schettino who took the cruise ship off course and rammed it into a reef off the Tuscan island of Giglio on January 13 Grosseto prosecutors are seeking indictments for Schettino and five other people on charges including manslaughter closed-door hearing is scheduled for Monday but it is not clear if the judge will make a decision then on whether to order a trial two other officials who were on the bridge during the grounding and the Costa official on land who was managing the crisis causing a shipwreck and abandoning the vessel before all the passengers had been evacuated Passengers have recounted a harrowing evacuation; by the time the captain ordered passengers to evacuate the ship was listing so far to one side that many lifeboats could not be lowered claiming it was his deft steering after the collision that allowed the ship to move closer to the port and helped to save lives He also maintained the reef was not marked on the ship’s navigational charts say the reef is a well-known tourist attraction in the pristine waters off Giglio remains on its side in Giglio’s port; efforts to right it and tow it away are under way From as little as €1 a week with our digital introductory offer Already a subscriber? 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