Michigan State University freshman center Inés Sotelo (15) scores a layup in the season-opening exhibition game against Wayne State University at the Breslin Center on Oct Michigan State women’s basketball freshman center Inés Sotelo is acclimating herself to her new scenery played basketball for Durán Maquinaria Ensino a professional women’s basketball club in the Spanish First Division In MSU's 95-49 win over Eastern Michigan Monday night, Sotelo shined with 13 points three rebounds and one steal for the Spartans No matter the difficulty, Sotelo is discovering her path living in a new country and learning a different style of basketball "I feel like I am finding my place here," Sotelo said "Each time I am making more things that I know In the beginning with not as much confidence in myself and now this time I’m getting more confidence Today I feel like I had a good game because I scored points and I felt more like myself." EMU forward Sisi Eleko is a major scoring threat as a big She tallied 25 points Monday night and was often Sotelo's defensive assignment that that'll be a good learning lesson for (Sotelo) but she's getting more and more comfortable," MSU head coach Robyn Fralick said "She has a really good feel and really good ball skills and athleticism with the way we play and press Three other Spartans scored in double-digits to help MSU remain in control the entire game Junior guard Theryn Hallock scored 18 points graduate guard Julia Ayrault scored 13 and redshirt freshman Kennedy Blair tallied 11 Graduate guard Jaddan Simmons led the team with seven rebounds and six assists The Spartans will be back in action Thursday 14 at Breslin Center to take on Eastern Kentucky Share and discuss “Inés Sotelo creating her own path with MSU women's basketball ” on social media they realised there were more problems than initially anticipated Trotter and his Studio’s co-founder Marcelo Martínez, took this on as their first personal interior design project together. The building renovation made using various traditional techniques and indigenous materials carefully preserves the historic features of the home as far as possible Martinez describes his thoughts when they started saying we were clear that the project had to feel cohesive and that the interventions had to relate to the structure and the place.” Rather than completely modernize it an artful balance of its existing elements and modern conveniences was achieved The internal atmosphere of the home was to be quaint yet relevant The home's entrance is through a courtyard that also doubles as the perfect spot to have breakfast and dinner as it does not receive direct sun This courtyard extends into the main living room and a fully equipped kitchen what once was the old chapel has been turned into a media room with its own powder room and outdoor shower Connected to the living room is a reflective dining room with another powder room and on the opposite edge is a garden room connected to a plunge pool Most of the ceilings on the ground floor are vaulted and around five meters high The upper level consists of three irregular bedroom suites The house is special because of its distinctive heritage qualities we tried to keep as many original elements and furniture as possible: most of the floors worked with artisans to make new external doors based on the original doors that were in the house the original items were saved wherever possible” The walls were refinished with handmade plaster which was locally acquired the colour palette was chosen in order to make some rooms feel light Once the plastering was complete and the floors cleaned the style of the house could be envisioned restored internal doors and a recreated main entrance all work in synergy to convince visitors that they were always a part of the house she enjoys writing about the way design influences culture She aspires to communicate the value of design through all her work From vernacular knowledge to modern sustainability Middle Eastern pavilions serve as living archives of architectural thought offering fresh frameworks for global adoption STIR engages with the curators of the Togo Oman and Qatar pavilions—debuting at the Venice Architecture Biennale 2025—on representation the book presents a fictional story depicting algorithms exercising control over humans and how this affects the built environment Tipnis shares how the toolbox democratises the practice of restoration via DIY resources to repair tangible urban heritage made of common building materials Exclusive preview for subscribers. Learn More Make your fridays matter. Learn More © Copyright 2019-2025 STIR Design Private Limited Please confirm your email address and we’ll send you a link to reset your password All your bookmarks will be available across all your devices Password must be 8 characters long including one capital letter By creating an account, you acknowledge and agree to the Terms of Use and Privacy Policy by STIR Select the Conversation Category you would like to watch Please enter your details and click submit Single account access for STIRworld.com,STIRpad.com and exclusive STIRfri content Verification link sent to check your inbox or spam folder to complete sign up process by Keziah Vikranth | Published on : Oct 28 Please enable JS and disable any ad blocker Please select what you would like included for printing: Copy the text below and then paste that into your favorite email application This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply Service map data © OpenStreetMap contributors Volume 12 - 2021 | https://doi.org/10.3389/fimmu.2021.778085 Type I Interferon Regulates the Survival and Functionality of B Cells in Rainbow Trout A Corrigendum onType I Interferon Regulates the Survival and Functionality of B Cells in Rainbow Trout By Benedicenti O, Wang T, Morel E, Secombes CJ, Soleto I, Díaz-Rosales P and Tafalla C (2020). Front. Immunol. 11:1494. doi: 10.3389/fimmu.2020.01494 In the original article, there was a mistake in Figure 1 as published. Although the numbers were correct, all dot plots in Figure 1A were the same. The corrected Figure 1 appears below Figure 1 Survival of blood IgM+IgD+ B cells in response to type I and type II IFNs 20 ng/ml rIFNg or media alone (control) and cultured at 20°C for 72 h Leukocytes were then labeled with specific monoclonal antibodies against trout IgM and IgD and analyzed by flow cytometry Cells were gated on the basis of their FSC and SSC and percentages of IgM+IgD+ cells determined on singlet and live (DAPI negative) cells Representative dot plots from one individual fish are shown (A) along with mean percentages and total number of cells detected for IgM+IgD+ B cells (B) and IgM−IgD− cells (C) (mean + SEM; n = 9) B cells were sorted from blood leukocytes using a biotinyilated Fab fragment of anti-IgM 1.14 and then incubated with the rIFNs as described above the percentage of live IgM+IgD+ B cells and the total number of live IgM+IgD+ B cells determined by flow cytometry as described in the Materials and Methods section (mean + SEM; n = 7) (D) Asterisks denote significant differences between samples treated with rIFNs and control samples (*P ≤ 0.05 The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher Díaz-Rosales P and Tafalla C (2021) Corrigendum: Type I Interferon Regulates the Survival and Functionality of B Cells in Rainbow Trout Received: 16 September 2021; Accepted: 29 September 2021;Published: 18 October 2021 Copyright © 2021 Benedicenti, Wang, Morel, Secombes, Soleto, Díaz-Rosales and Tafalla. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) distribution or reproduction in other forums is permitted provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited in accordance with accepted academic practice distribution or reproduction is permitted which does not comply with these terms *Correspondence: Carolina Tafalla, dGFmYWxsYUBpbmlhLmVz Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher 94% of researchers rate our articles as excellent or goodLearn more about the work of our research integrity team to safeguard the quality of each article we publish In some areas of the house the ceilings reach a height of more than 16 feet The 18th-century walnut dining table was purchased from Le Icone in Cisternino All the carpets in the house are by Armadillo Rugs Casa Soleto is located in the municipality with a population of 5,000 that has the same name as the house equidistant from the Ionian and Adriatic seas This part of Italy is an unusual linguistic island known as Salentinian Greece and many residents still speak Griko a variant of Greek that exists only here and in a corner of Calabria a map dating to around 500 BCE and representing ancient Salento was found engraved on a fragment from a terracotta vase “Children play outside and old men argue in the street while their wives make pasta” “It’s like being in an old Italian film.” The old medieval village retains its original layout with narrow streets lined with distinguished buildings Studio Andrea Trotter and Marcelo Martínez collaborated with Domingue Finishes on the walls “They make some of the most beautiful plasters we have ever seen The application was carried out by Tullio Cardinale and his team The façade of the house was restored to its original state it had a façade with baroque details and an interior with high vaulted ceilings and old family photos—all just as they had been left more than 20 years earlier “It seemed like all the house needed was a little love and we could have moved in right away The restoration took longer than expected and required replacing the roof and removing layers of concrete from the walls starting with adding a bathroom for each bedroom much of the original house was in good condition Some parts of it are 400 years old yet required little intervention on the part of the designers there is a chapel that has now been converted into a media room Also read: Designer Namrata Datt's 1,400-square-foot Delhi home is a Parisian paradise were restored by Alba Falegnameria (Lecce) The restoration involved several local artisans who were passionate participants in the work and you have to go through the front patio and up the outside staircase to reach the bedrooms There are three spacious bedrooms on the first floor: one overlooks the garden On the ground floor there is a green room designed for afternoon tea from which there is access to the porch and from there to the garden with a swimming pool there are many different rooms and spaces waiting to be discovered.” Hanging on the walls are paintings that came with the house while other works have been specially created by Eleanor Herbosch There are also a number of pieces by Swedish artist Lucas Morten Studio Andrew Trotter has a number of projects in Puglia His interest in the region started about nine years ago when a friend asked Trotter to visit with him in search of a building to renovate the friend called to tell him that he had found a property while asking for Trotter’s help with its renovation While Trotter had studied interior design in Australia he didn’t pursue a career in the field until later in life (Instead his resume’s highlights included some unusual stints among them a year working with Australian actress Anouska Hempel.) Still in 2010 he established Openhouse and then Openhouse Magazine followed in 2014 Trotter said he would take on the project and The success of that first commission led to others starting with Villa Cardo and Villa Castelluccio met in Barcelona and immediately began collaborating deciding shortly afterwards to open Studio Andrew Trotter together Casa Soleto is their first “personal project” One of four bedrooms with a late-18th/early-19th-century wardrobe from Lombardy Casa Soleto differs somewhat from Trotter’s other buildings in Puglia: the walls were painted with lime to achieve a seductive patina that resonates with the history of the building “We wanted to move away from the typical white interiors we usually do here It is the ideal place to stay cool on hot days to linger with friends or to relax while reading a book” while explaining how he laid several jute carpets they help to make this an oasis with a peaceful ambience Also read: A private rooftop in Hyderabad designed for memorable sundowners The church of Soleto. From here, it only takes 25 minutes to reach the sea while not far away is Galatina, Soleto’s twin with many centuries-old baroque buildings. One of the bathrooms, with an enamelled iron bathtub. The deep pool in the back garden is the perfect place to relax and cool down. Being close to the church adds a certain sense of authenticity: “It's magical to wake up to the sounds of birds and bells!” says Trotter. And then the day begins in the courtyard in front of the kitchen. “It’s still cool in the morning, and it’s the perfect place to have coffee and breakfast.” All at a leisurely pace, of course. SALVA LOPEZ1/11The 18th-century walnut dining table was recovered from a monastery in Abruzzo and purchased at Le Icone in Cisternino. SALVA LOPEZ2/11Blasco sofas and armchairs. SALVA LOPEZ3/11Armadillo Rugs. The most modern elements in the house come from Frame. SALVA LOPEZ4/11In the kitchen, the original cabinets were retained while new ones in a similar design were installed to hide the appliances. ILVE appliances and range. SALVA LOPEZ5/11The external staircase in the courtyard joins the living area to the bedrooms. SALVA LOPEZ6/11The view of the pool from one of the bedrooms. SALVA LOPEZ7/11A corner of the children’s bedroom upstairs. SALVA LOPEZ8/11For the bathrooms, Trotter and Martínez chose Valadares fixtures. SALVA LOPEZ9/11In the small pink bathroom, a custom-made washbasin by Arte Ippolito. SALVA LOPEZ10/11Many of the paintings were purchased with the house; to these the owners added works by their friend Eleanor Herbosch, a young artist from Antwerp. SALVA LOPEZ11/11The desk in one of the rooms. The material on this site may not be reproduced except with the prior written permission of Condé Nast Jackson County Sheriff's Office deputies arrested Jose "Alfredo" Sotelo-Palma after receiving a tip about his potential location Officers found Sotelo-Palma around 8:30 p.m Friday evening at a property in the 2900 block of Eastside Road in rural Jacksonville The location is approximately four miles away from where the alleged murder occurred Soleto-Palma was arrested without incident and is lodged at the Jackson County Jail without bail JCSO thanks the community for the information that let to his arrest Jackson County Sheriff's Office (JCSO) detectives are asking for help in finding a suspect wanted for murder Jose "Alfredo" Sotelo-Palma from Sinaloa Mexico is wanted in connection with the shooting homicide Monday Detectives say the homicide happened at a marijuana growing and processing facility outside Jacksonville The victim was identified as Luis Ayala-Zavala Sotelo-Palma is facing charges of second-degree murder Anyone with information on his location is asked to call dispatch at 541-776-7206 If you have more information on the suspected homicide call the JCSO tip line at 541-774-8333 and reference case number 22-6947 No further information is available at this time Metrics details As B cells are singularly equipped with a B cell receptor (BCR) and a range of innate receptors they are able to integrate both antigen-specific and innate signals with the latter being essential to reach an adequate level of activation Whether teleost B cells sense pathogens through innate mechanisms has not yet been explored despite the fact that fish B cells display a wider array of innate receptors than many mammalian B cell subsets we have investigated the effects of inactivated Aeromonas salmonicida on trout splenic IgM+ B cells in vitro in the presence or absence of different inhibitors of Toll-like receptor (TLR) signalling to establish to what degree innate signals are contributing to the activation of B cells in teleosts Our results demonstrate that most of the effects that A salmonicida exerts on trout IgM+ B cells are significantly blocked in the presence of inhibitors of MyD88 and TRIF TLR signalling is essential for the activation of IgM+ B cells These results will be useful for the future optimization of novel vaccines and adjuvants highlighting the great potential of TLR ligands as adjuvants Although commercial vaccines are able to induce long-term protection furunculosis outbreaks are still frequent in several fresh and marine aquacultured species which provide novel information regarding the mechanisms through which fish B cells recognize bacteria and become activated will surely be valuable for the future optimization of novel prevention strategies against this and other pathogenic bacteria salmonicida previously labelled with Syto BC Green at a 1:2 cell:bacteria ratio cells were stained with anti-IgM (shown as red) and plated onto poly-L-lysine coated glass slides Samples were then analysed by confocal fluorescence microscopy Representative confocal microscopy images include a large field (top images) and a higher magnification (lower images) showing both an IgM+ B cell and an IgM- cell phagocyting A 10 µm on the large fields and 2 µm on the higher magnifications) Splenic leukocytes were incubated with MyD88 inhibitor peptide (100 µM) the same volume of DMSO or media alone for 1 h Controls without bacteria were also included cells were stained with anti-trout IgM-APC and analysed by flow cytometry Representative dot plot from one individual fish is shown (b) along with the quantification of the percentage of phagocytic IgM+ B cells (cells in the upper right quadrant) among total IgM+ cells (cells in upper quadrants) after each treatment (mean + SD; n = 7 individual fish) (c Asterisks denote significant differences between groups as indicated (*P ≤ 0.05) A. salmonicida increases IgM+ B cell survival and has lymphoproliferative effects. Splenocytes were incubated with the different inhibitors or left unstimulated as described in the legend of Fig. 1 salmonicida at a ratio 1:2 (cell:bacteria) cells were labelled with anti-trout IgM-APC and the percentage of IgM+ B cells in the cultures determined by flow cytometry together with a quantification of the percentage of IgM+ B cells in cultures (mean + SD; n = 12) (b,c) salmonicida on B cells were determined in parallel cells were pre-treated with the inhibitors and then stimulated with the bacteria as described above splenic leukocytes were incubated with EdU for an additional 24 h cells were labelled with anti-trout IgM-APC and the percentage of proliferating cells determined as described in the Materials and Methods section together with a quantification of the percentage of proliferating IgM+ B cells (mean + SD; n = 9) (e,f) Asterisks denote significant differences between groups as indicated (*P ≤ 0.05; ***P ≤ 0.005) A. salmonicida increases the expression of surface MHC II on IgM+ B cells. Splenocytes were incubated with the different inhibitors or left unstimulated as described in the legend of Fig. 1 cells were labelled with anti-trout IgM-FITC and anti-trout MHC II-APC and analysed by flow cytometry Representative dot plots (a) and histograms (b) showing MHC II expression levels in IgM+ B cells from one representative fish are included along with the quantification of MHC II mean fluorescence intensity (MFI) values in IgM+ B cells (c) and IgM- cells (d) (mean + SD; n = 12) Asterisks denote significant differences between groups as indicated (*P ≤ 0.05; **P ≤ 0.01) A. salmonicida differentiates B cells to IgM-secreting plasmablasts. Splenocytes were incubated with inhibitors or left unstimulated as described in the legend of Fig. 1 cells were plated into ELISPOT plates previously coated with anti-trout IgM and incubated for a further 24 h cells were washed and a biotinylated anti-trout IgM used to detect number of spot forming cells Images from a representative fish are shown (a) together with a quantification of the number of IgM-secreting cells (mean + SD; n = 12) (b) Splenocytes pre-treated with the inhibitors salmonicida were also analysed by flow cytometry after 72 h of incubation with the bacteria IgM+ B cells were gated and the MFI of their forward scatter (FSC) Representative histograms are shown (c) along with a quantification of FSC MFI values in IgM+ B cells (mean + SD; n = 12) (d) splenocyte cultures were stimulated with A IgM+ B cells were isolated by flow cytometry RNA extracted and the levels of transcription of Blimp-1 and Pax5 determined by real time PCR as described in the Materials and Methods section Expression relative to the endogenous control EF-1α was calculated for each sample Asterisks denote significant differences between groups as indicated (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.005) salmonicida or an amount of LPS that corresponded to that same number of bacterial cells for 3 days at 20 ºC cells were labelled anti-trout IgM-APC and analysed by flow cytometry to estimate the percentage of IgM+ B cells (a) Representative dot plots are shown together with a quantification of average IgM+ B cells in cultures (mean + SD; n = 12) (b) At the same time the lymphoproliferative effect was measured after incubating the splenocytes with EdU for a further 24 h cells were labelled with anti-trout IgM-APC and number of proliferating cells determined as described in Materials and Methods Representative dot plots are shown (c) together with a quantification of proliferative IgM+ B cells (mean + SD; n = 9) (d) The expression of MHC II on the surface of IgM+ B cells was also measured after the incubation with A Representative plots and histogram are shown (e) together with a quantification of the mean intensity fluorescence of MHC II on the surface of IgM+ B cells (mean + SD; n = 12) (f) we thought of great interest to establish to what degree TLR signalling was contributing to the activation of B cells in this species an important rainbow trout pathogen that continues to cause mass mortalities in salmonid aquaculture worldwide To rule out any possible bacteria-mediated effects This might explain why most of the reversions of A salmonicida-induced activation of B cells were much stronger in the case of resveratrol than in response to the MyD88 inhibitor it should be noted that the precise effects of the two inhibitors used in this study in downstream TLR signalling has never been established in teleost fish due to a lack of specific reagents although the work presented in this paper represents sufficient evidence of how both inhibitors are capable of blocking inflammatory responses also in fish it might be possible that these inhibitors are not 100% efficacious in fish or that they have effects slightly different to those reported for mammalian cells the receptor that is mediating these effects in B cells is still unknown and is an issue that should be addressed in future studies only resveratrol and not the MyD88 inhibitor was capable of significantly reverting this effect Whether this is a consequence of surface MHC II expression up-regulation requiring the internalization of the bacteria or because this effect is mediated through a TRIF-dependent mechanism is still undetermined and deserves further investigation we found that the bacterial LPS on its own was capable of inducing higher levels of surface MHC II expression in IgM+ B cells than those observed in response to the intact bacteria these results suggests that an internalization of the bacteria is not required to induce surface MHC II expression it seems plausible to hypothesize that TLR engagement plays a quite prominent role in teleost B cells This hypothesis seems confirmed by the fact that the degree of reversion exerted by the TLR inhibitors on the effects provoked by A salmonicida on trout IgM+ B cells was quite high we have demonstrated that upon recognition of inactivated A rainbow trout IgM+ B cells increase in number increase surface MHC II expression and differentiate towards IgM-secreting cells the MyD88 inhibitor significantly reverted the increased IgM+ B cell survival and the up-regulated IgM secretion whereas resveratrol significantly reverted the higher surface MHC II levels and the increased IgM secretion These results highlight a large contribution of TLR signalling in the activation of B cells by the bacteria salmonicida LPS by itself has stronger effects on MHC II surface expression than the complete bacteria while having lower lymphoproliferative effects Understanding how fish B cells sense antigens is essential for the development of effective vaccines and adjuvants therefore our work will hopefully contribute to the design of a more effective A fish were maintained at the Animal Health Research Centre (CISA-INIA) laboratory at 16 ºC with a re-circulating water system and 12:12 h light:dark photoperiod Fish were fed twice a day with a commercial diet (Skretting fish were acclimatized to laboratory conditions for 2 weeks and during this period no clinical signs were ever observed The experiments described comply with the Guidelines of the European Union Council (2010/63/EU) for the use of laboratory animals and were previously approved by the Ethics committee from the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA; Code CEEA PROEX002/17) Invitrogen) supplemented with 100 I.U./ml penicillin and 100 µg/ml streptomycin (P/S 10 units/ml heparin (Sigma) and 5% foetal calf serum (FCS Cell suspensions were placed onto 30/51% discontinuous Percoll (GE Healthcare) density gradients and centrifuged at 500 × g for 30 min at 4 ºC The interface cells were washed twice in L-15 with 2% FCS and cells were resuspended in L-15 with 5% FCS The viable cell concentration was determined by Trypan blue (Sigma-Aldrich) exclusion adjusting the concentration to 2 × 106 cells/ml salmonicida CECT4237 was aerobically grown in Tryptone Soya Broth (Oxoid) at 25 ˚C To stimulate the rainbow trout splenocytes salmonicida grown in broth overnight to exponential phase was heat-inactivated at 65 °C for 1 h salmonicida was used to stimulate the cells LPS was isolated using a commercial extraction kit (iNtRON Biotechnology) following the manufacturer’s protocol The absence of DNA and protein contamination was confirmed by SDS-PAGE and agarose electrophoresis A MyD88 inhibitor peptide was purchased from Novusbio and used at a concentration of 100 µM A control peptide was used as a control at the same concentration was diluted in DMSO and was used in cells at a final concentration of 50 µM the same volume of DMSO was added to cell cultures as a negative control Flow cytometry analysis was performed with FlowJo 10 (TreeStar) cells labelled with anti-trout IgM-APC (5 µg/ml) were washed with serum-free L-15 medium Laser scanning confocal microscopy images (0.3 μm thickness) were acquired with an inverted Zeiss Axiovert LSM 880 microscope Images were analysed with Zen 2.0 (Carl Zeiss) and Fiji (NIH) software packages splenocytes at a concentration of 2 × 106 cells per ml were incubated for 1 h with the different TLR inhibitors their controls or media alone as described above The cells were then stimulated with inactivated A salmonicida for 3 days at 20 ºC as described above or left unstimulated EdU (1 µM) was added to the cultures and the cells were incubated for an additional 24 h cells were collected and stained with anti-trout IgM-APC (0.5 μg/ml) cells were then fixed and permeabilised with Cytofix/Cytoperm buffer for 15 min at room temperature (RT) the incorporation of EdU to the DNA was detected following the manufacturer´s instructions and then analysed by flow cytometry on a FACS Celesta flow cytometer Splenocytes (2 × 106 cells/ml) were incubated with the different TLR inhibitors salmonicida as described above for 48 h at 20 ºC Cells (5 × 104 cells per well) were then transferred to ELISPOT plates pre-coated with anti-trout IgM (2 µg/ml) cells were washed away 5 times with PBS and plates were blocked with 2% BSA in PBS for 1 h at RT biotinylated anti-trout IgM was added to the plates and incubated at 1 µg/ml for 1 h at RT Following additional washing steps (5 times in PBS) the plates were developed using streptavidin-HRP at 100 ng/ml (Thermo Fischer Scientific) at RT for 1 h washed again with PBS and incubated with 3-amino 9-ethylcarbazole (Sigma-Aldrich) for 30 min at RT in the dark Substrate reaction was stopped by washing the plates with tap water the number of spots in each well was determined using an AID iSpot Reader System (Autoimmun Diagnostika GmbH) IgM+ B cells populations were isolated by flow cytometry in a BD FACSAria III cell sorter (BD Biosciences) after staining spleen leukocytes with anti-trout IgM-APC as described above using their FSC/SSC and fluorescence characteristics In this case 7-AAD (BD Biosciences) at 2.5 µg/ml was used to check the cell viability Each sample was measured in duplicate under the following conditions: 10 min at 95 ºC followed by 40 amplification cycles (15 s at 95 ºC and 1 min at 60 ºC) A melting curve for each primer set was obtained by reading fluorescence every degree between 60 ºC and 95 ºC to ensure only a single product had been amplified The expression of individual genes was normalized to the relative expression of trout housekeeping gene elongation factor 1α (EF-1α) and the expression levels were calculated using the 2-ΔCt method where ΔCt is determined by subtracting the EF-1α value from the target Ct No template negative controls and minus reverse transcriptase controls were included in all the experiments Statistical analyses were performed using the Graphpad prism version 6 (Graphpad software) All values were verified to be normally distributed one-way ANOVA followed by Tukey’s test as a post-hoc was performed The differences between the mean values were considered significant on different degrees Pattern recognition receptors in innate immunity TLR signaling in B-cell development and activation The role of pattern-recognition receptors in innate immunity: Update on Toll-like receptors Toll-like receptors–sentries in the B-cell response LPS stimulates IgM production in vivo without help from non-B cells Differential regulation of TLR4 expression in human B cells and monocytes A role for Toll-like receptors in acquired immunity: Up-regulation of TLR9 by BCR triggering in naive B cells and constitutive expression in memory B cells The establishment of early B cell tolerance in humans: Lessons from primary immunodeficiency diseases Toll-like receptor 7 controls the anti-retroviral germinal center response Selective utilization of Toll-like receptor and MyD88 signaling in B cells for enhancement of the antiviral germinal center response Toll-like receptor recognition of bacteria in fish: ligand specificity and signal pathways Ligand specificities of Toll-like receptors in fish: Indications from infection studies Transcriptional heterogeneity of IgM(+) cells in rainbow trout (Oncorhynchus mykiss) tissues Distinct differentiation programs triggered by IL-6 and LPS in teleost IgM(+) B cells in the absence of germinal centers Profiling Atlantic salmon B cell populations: CpG-mediated TLR-ligation enhances IgM secretion and modulates immune gene expression CpG oligodeoxynucleotides modulate innate and adaptive functions of IgM(+) B cells in rainbow trout Aeromonas salmonicida: Updates on an old acquaintance Resveratrol modulates phagocytosis of bacteria through an NF-kappaB-dependent gene program Blimp-1 orchestrates plasma cell differentiation by extinguishing the mature B cell gene expression program B cell-activating factor regulates different aspects of B cell functionality and is produced by a subset of splenic B cells in teleost fish Regulation of IgM(+) B cell activities by rainbow trout APRIL reveals specific effects of this cytokine in lower vertebrates TLR agonists selectively promote terminal plasma cell differentiation of B cell subsets specialized in thymus-independent responses Identification of the first teleost CD5 molecule: Additional evidence on phenotypical and functional similarities between fish IgM(+) B cells and mammalian B1 cells B lymphocytes from early vertebrates have potent phagocytic and microbicidal abilities Different IgM(+) B cell subpopulations residing within the peritoneal cavity of vaccinated rainbow trout are differently regulated by BAFF Control of B-cell responses by Toll-like receptors Adjuvant-enhanced antibody responses in the absence of toll-like receptor signaling Toll-like receptors and innate immunity in B-cell activation and antibody responses Phylogeny of lower vertebrates and their immunological structures Identification of teleost skin CD8alpha+ dendritic-like cells representing a potential common ancestor for mammalian cross-presenting dendritic cells Download references This work was supported by the European Research Council (ERC Consolidator Grant 2016 725061 TEMUBLYM) Innovation and Universities (project AGL2017-85494-C2-1-R) and by the Comunidad de Madrid (grant 2016-T1/BIO-1672) The authors want to acknowledge Lucía González and Diana Martín for technical support Patricia Díaz-Rosales & Carolina Tafalla that also interpreted the results with help from P.D.R The authors declare no competing interests Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41598-020-73999-w Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily. This website is using a security service to protect itself from online attacks. The action you just performed triggered the security solution. There are several actions that could trigger this block including submitting a certain word or phrase, a SQL command or malformed data. You can email the site owner to let them know you were blocked. Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page. Homer football coach Glen Kyle couldn’t believe the eventual diagnosis he received when he entered Minden Medical Center a few weeks ago with a painful appendix but not many end up with a cancer diagnosis after the pesky organ is removed Only about a thousand cases of appendix cancer are diagnosed annually in the United States a Homer native and longtime coach at Homer and Ruston thought he was in the clear after having his appendix removed on a recent Monday and returning to football before the end of that week Kyle though Soleto was talking about what could have happened to him “It was as if I was falling through space Soleto quickly corralled my thoughts and told me there was no need for a will and testament just yet,” Kyle said.  But the doc scheduled a colonoscopy for the following Monday The bad news came when Kyle received a call from an oncologist/hematologist scheduling an appointment.  I was not sure what a hematologist was but I dang sure knew what an oncologist is,” he said of a doctor who deals with cancer treatments All of this happened before Homer’s season opener with Delta Charter There’s been doctor appointments and surgeries while trying to take care of coaching duties to make sure a promising Pelicans team remains on track “The first person I told when I found out what I was about to go through was not my wife or son or mother -- I told my athletic director Elaine Gilbert,” Kyle said “She brought me back down to earth and told me how she handled her fight with cancer several years ago but going through something like this makes you really put your life in order.” Since the appendix was attached to the outside of Kyle’s colon the oncologist decided it would be best to remove that part of his colon he would have told the doctors to wait until after the end of football season to have the surgery. “Not anymore so I would not have that lingering over me all season,” he said Gilbert said Homer’s four assistant coaches have been staying at the field house later and staying longer on weekends to pick up Kyle’s slack.  “Our school and town always seems to push through during tough times,” Gilbert said “The coaches are encouraging each other because that’s what you do when one of your own is not able to be with you This crisis in coach Kyle’s life is tough for his family and extended school family but it’s also bringing this Pelican family together Remember: God does not put on you more than you can handle.” who played at Homer for the late Ronnie Beard and Wayne Volentine missed Friday’s 26-12 victory over Beekman Charter which moved the Pelicans to 2-0 on the season His son Facebook-lived the game so he could watch it Tuesday he received the results of his lab work and was told that everything came back negative – no more cancer.  that cancer could have spread throughout my colon undetected,” Kyle said I had to go in to get mine removed.” has 37 staples in his gut and hopes to return in some capacity in a couple of weeks he gets daily practice reports from offensive coordinator Richie Casey while worrying about what lies ahead for the Pelicans who host Vidalia this week and have Jonesboro-Hodge Parkway and Haynesville later on the schedule.  “My assistants are putting the time and effort in,” he said “We have some tough games down the line but I am very pleased with where we are now.” "Tales of fire" is a photographic project that addresses the complex phenomenon of fires in Salento the Salento territory was characterized by a flourishing monoculture of olive trees due to the spread of the Xylella fastidiosa bacterium which appeared under unclear circumstances olive trees began to suffer from a disease called Rapid Desiccation Complex it is estimated that the infected plants in the area are more than 21 million The most obvious consequence is a landscape that has turned from green and lush into an endless expanse of dead trees In a territory in progressive depopulation and withering fires have found their sustenance: dry olive trees burn like incandescent torches generating an unprecedented environmental disaster in the last twenty years their frequency and intensity has increased dramatically the reasons given are many: abandonment of the land by new generations; use of fire as an cheap method to free land from dried up olive trees these factors alone are not enough to explain a phenomenon of such vastness The real reasons may have much deeper economic roots and would be linked to the public funding for agriculture and to the public funding for the uprooting and replanting of diseased olive trees with more productive varieties according to several testimonies collected in many cases fire is a way to depreciate a land that is already on sale or the tool used to force the owners to sell their land Behind Salento there seem to be different interests from those related to new projects of super-intensive olive oil production which would make Italy able to compete again on the international market by holding up the competition with other countries (such as Spain or Tunisia) to those related to bioeconomy and energy transition and to go up in smoke is not just the territory but also a centuries-old agricultural tradition where the small owner was the guardian of the land Veronica Andrea Sauchelli and Valentina Borgato burning olive trees produce the characteristic "chimney effect" continuing to burn from the inside and becoming impossible to extinguish Italy An old sickle used for cleaning the fields Nowadays these tools have been replaced by very powerful and harmful chemical herbicides responsible for the impoverishment of the soil and closely connected to the proliferation of Xylella After a summer in which the fires have tripled it has begun to believe that the olive trees were burned to create political pressure to release public funding for the removal of dead trees has declared that "Fire is not a method" but has increased funding from 6 to 60 million After seeing the flames enter her land twice Chiara launched the petition "Save the olive trees of Salento" collecting more than 37 thousand signatures Italy A lamp from Chiara's biodynamic forest one of the founding members of Karadrà: an innovative agricultural cooperative whose fields were set on fire the fire was set for intimidation purposes Lecce An olive tree in flames during a fire that involved more than a thousand trees Lecce A window of an abandoned "masseria" The masserias in Apulia are rural buildings that were used by the agricultural activities to store objects and tools for daily use of work in the countryside but were also used as warehouses for food (such as fodder) or used as stables for livestock they were often used as houses from shepherds and farmers during the period of major agricultural work For this reason the name "masseria" derives from "masserizie" that is a place where various things were contained Today most of the masserias are being abandoned Italy The fumes of a large fire in a private property in fact they believe that the authors were people who came the day before to bargain to buy the land sings under an olive tree during a typical farmer's feast in a private masseria This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page Periphery have been on a creative roll Only a year and half after 2015‘s Juggernaut: Alpha and Juggernaut: Omega were released band is back with their fifth full-length album, Periphery III: Select Difficulty Select Difficulty was not initially supposed to be a full-length “Juggernaut was so long and comparatively stressful,” says guitarist Misha Mansoor The time crunch actually helped it become the most cohesive too Periphery III: Select Difficulty clocks in at more than an hour Unlike the extended intros of past records this time around the band gets right down to business with “The Price Is Wrong.” It’s intense and aggressive but also packed with clever riffs and their trademark djent tendencies The barrage continues with “Motormouth.” Vocalist Spencer Soleto really brings it on this album That’s encapsulated in the epic “Marigold,” where he displays a variety of vocal styles from harsh yells to melodic croons to a surprisingly effective falsetto The track is augmented by orchestral elements which add an interesting atmosphere Those synths and orchestral elements are used throughout the album but inserts enough to give the songs additional depth and texture The last minute plus of “Absolomb” is very cinematic Periphery’s triple-guitar attack is known for their technical prowess but they also help make the album a cohesive one Jake Bowen and Mark Holcomb blend a variety of riffs with creative fills that are sometimes powerful and heavy One of the highlights of the later part of the album is “Prayer Position,” a concise and sometimes brutal track that’s balanced by a catchy melodic chorus The album wraps up with “Lune,” an atmospheric ballad that gathers momentum before a mellow Periphery III: Select Difficulty is an impressive album The band’s multi-faceted musicianship hits on all cylinders with the arrangements showing a lot of diversity While not as experimental as some of their past releases Periphery still push plenty of musical boundaries making it a challenging and satisfying release Read our review of Periphery's new album 'Periphery III: Select Difficulty.'\nRead More Periphery have been on a creative roll Romanian non-profit green-tech firm Justin Capra Foundation for Sustainable Technologies and Inventions (or FITS) has unveiled the Soleta zeroEnergy range of sustainable off-grid homes The flexible dwellings are available in several shapes and sizes and combine rustic modular design with the proverbial kitchen sink of energy saving and producing technologies The Soleta zeroEnergy range comprises several homes ranging from a small unit with just 48 square-meters (516 sq ft) usable floor space (plus attic) up to a relatively sizable model suitable for a family of five with a main area floor space of 100 square-meters Clever space-saving storage solutions will help make the most of available space While the name may imply that these homes have no energy needs they've actually been designed to utilize renewable energy sources to allow residents to jump feet-first into the off-grid lifestyle modern amenities like running water and electricity can be hooked-up if desired Depending on need and budget, each zeroEnergy home can incorporate a geothermal water heating system, wind power, solar power, and water collection. Large low-e windows and LED lighting feature throughout and a pellet-burning stove is also available for cooler climes A computer monitoring system helps keep everything in check The houses are reportedly manufactured from 97 percent recycled materials with wood being the primary material of choice and so if space does eventually become an issue an extra section should be relatively simple to add The homes can be built on either wooden foundations or existing concrete foundations at lower cost The Soleta zeroEnergy range of homes are by no means the only viable fully off-grid abodes we’ve covered here at Gizmag, and Studio H:T’s effort springs to mind as an appealing alternative The range starts at €25,000 (roughly US$32,000) for the smallest zeroEnergy One with the larger zeroEnergy Two unit commanding a cool €57,000 A ten-year warranty is included with the sale and FITS is currently exhibiting a show house outside the US embassy in Bucharest Source: Soleta (in Romanian but a company rep informed us that English-language content will be added soon) VancouverNews'We are broken in pieces': Family of man killed in Vancouver roof collapse wants answersBy Tahmina AzizPublished: July 16, 2022 at 12:06PM EDT Twitter feed ©2025 BellMedia All Rights Reserved