The following is a summary of “Evaluated NSUN3 in reticulocytes from HbH-CS disease that reflects cellular stress in erythroblasts,” published in the April 2025 issue of Annals of Hematology by Liu et al Hemoglobin H Disease-Constant Spring (HbH-CS) is a severe α-thalassemia variant caused by reduced α-globin synthesis It leads to oxidative stress from excess β-globin chains and unstable Hemoglobin H (HbH) formation Researchers conducted a retrospective study on HbH-CS a severe α-thalassemia variant marked by reduced α-globin synthesis and excess β-globin aggregation elevated reactive oxygen species (ROS) and malondialdehyde (MDA) levels and activated the Nrf2- antioxidant response element (ARE) antioxidant response They reported abnormal NSUN3 overexpression in reticulocytes from patients with HbH-CS and constructed an in vitro NSUN3 overexpression/silencing model using K562 cells with erythroid differentiation potential and an intact bead protein gene cluster The results showed that NSUN3 overexpression significantly increased intracellular ROS and MDA levels reduced GSH content and diminished the overall cellular antioxidant capacity (T-AOC) and inhibited the synthesis of mitochondrial respiratory chain complexes I and IV through aberrant m5C→f5C modification while NSUN3 silencing produced the opposite effects Investigators provided initial insights into how NSUN3 modulated oxidative stress in erythrocytes through epigenetic modifications They contributed to understanding the clinical management of patients with HbH-CS Source: link.springer.com/article/10.1007/s00277-025-06359-1 The latest articles and insights from your colleagues in your specialty(ies) of choice SUBSCRIBE NOW View all newsletters Insights from the leaders in medical research Subscribe to our free Newsletters to receive weekly emails and even get a laugh or two from our medical cartoons SUBSCRIBE NOW About Physician’s Weekly Careers Memberships & Verifications Advertise With Us Our Partners Blog Terms & Conditions Privacy Policy Editorial Policy Contact Us The content of this site is intended for healthcare professionals Any views and opinions expressed are those of the author(s) and/or participants and do not necessarily reflect the views A profile showcasing an individual's professional experience A profile for companies that LinkedIn users can follow Constant Spring FC delivered a dominant performance in the Kingston and St Andrew Football Association (KSAFA) Wray and Nephew Championship League on Saturday securing a commanding 4-0 victory over Boys’ Town FC Javier Wilmott and Colieke Richards found the back of the net in a relentless attacking display ensuring their side walked away with all three points Duhaney Park FC outscored Maverley/Hughenden FC 4-2 in an entertaining encounter and Kamar Reid were on target for Duhaney Park Marverly/Hughenden struggled to contain their opponents’ offensive firepower Shortwood United and Olympic Gardens battled to an exciting 2-2 draw with both teams creating chances in a hard-fought contest Alex Bagalue and Malik Morris struck for Shortwood while Olympic Gardens responded through Cheanardo Anderson to ensure they shared the spoils JDF FC earned a solid 2-0 win over Browns Town FC with goals from Te-Jaurn Williams and Terwayne Foster securing the result JDF’s well-structured play saw them control the game limiting Browns Town’s attacking opportunities Real Mona FC continued their winning form with a 2-0 victory over August Town FC thanks to goals from Davonique Gould Bourne and Nashordo Gibbs Mona’s defensive organization and sharp finishing made the difference as August Town struggled to mount a comeback Barbican FC edged Santos FC 1-0 in a closely contested affair with Davian Shakes providing the lone goal of the match but Barbican’s resilience saw them come out on top The St Andrew North police are probing the murder of a man who was shot and killed by men travelling on motorcycle this morning along Constant Spring Road Our news team was reliably told that a Mercedes Benz was shot up in the vicinity of Phil’s Hardware One of the occupants was taken to Andrews Memorial Hospital where he was pronounced dead Investigators revisited the crime scene this morning for a clearer understanding and commenced a probe which is located between Shortwood Road intersection and Dunrobin Avenue is still active so motorists are advised to use alternative routes Follow The Gleaner on X, formerly Twitter, and Instagram @JamaicaGleaner and on Facebook @GleanerJamaica. Send us a message on WhatsApp at 1-876-499-0169 or email us at onlinefeedback@gleanerjm.com or editors@gleanerjm.com View the discussion thread. Close Donate now: Support recovery from severe storms across the U.S. across the plains and even in states that may have never experienced the repeated onslaught of damaging storms that we’ve witnessed this year causing massive destruction and injuring several people where we know some homes have flooded multiple times just over the past few months But this is not a new phenomenon. Over the years, we’ve often highlighted the disparity between “named” disasters that receive an outpouring of national media attention and philanthropic contributions, and low-attention disasters whose toll on communities is immeasurable and yet receive discouragingly small amounts of philanthropic support The recent barrage of severe weather events has made this even more prominent But community-based organizations that are normally preparing to support needs that may arise from a hurricane later in the summer are now having to mobilize to help people already affected by spring storms We see this happen often with low-attention disasters in rural communities but it’s crucial to remember that just because some of the communities affected by recent severe weather are more rural or not densely populated doesn’t mean the impact of the storms is less severe these smaller communities simply do not have the resources or infrastructure needed to support recovery and find it difficult to access additional resources from the federal government As the severe storms keep coming and more communities are affected, it’s our job as funders to combat the disaster fatigue, see past the media coverage and support the communities that need us most. I encourage all funders to lean into the idea that every funder is a disaster funder in an effort to mitigate the impact of disasters and ultimately build stronger At CDP, our mission is to “mobilize philanthropy to strengthen the ability of all communities to withstand disasters and recover equitably when they occur.” It’s time to mobilize because all communities are not recovering equitably right now Join us in supporting communities like those I’ve mentioned above If you want to mobilize to help communities recover from the recent onslaught of severe weather I’m happy to help you better understand immediate and long-term needs and how we can collaborate to support equitable recovery for all Give with confidence The Center for Disaster Philanthropy is a 501(c)(3) charity that has earned Charity Navigator’s four-star rating and Candid’s Platinum Transparency seal Made with by creatives with a conscience Not a single smartphone or tablet was in sight as students from Constant Spring Primary and Infant School some dressed as their favourite literary characters came out to participate in a literacy fair held at the institution’s campus in St spearheaded by the school’s Literacy Committee under the theme ‘Unlock Your Superpower Read!’ featured a variety of educational and engaging activities It was aimed at fostering a love for reading among students in an interactive way while also highlighting the importance of literacy in their overall academic success Organiser of the event and Literacy Coordinator at the school told JIS News that the fair served to highlight the various aspects of reading and how to get students engaged “Our aim [was] to bring awareness to students that reading is not just siting [down] with a book “We have students here who have done drawings from stories they have written alternative endings to stories They have made items from stories that they have read [and they are] on display for parents and visitors to see,” she noted citing the exhibitions at the various classroom booths Among the students celebrating the joys of reading was grade-five student who was dressed as ‘Tiger Lily’ from Peter Pan I acted out the character and I really felt like I was actually the character,” she told JIS News makes reading more fun and engaging for children It helps to foster imagination and creativity and build their confidence by expressing themselves through role-playing “If reading was interactive students would be reading every single day because they would learn new words and they would get smarter,” Asia said Sharing an example from a book she has read she said in ‘Think Big’ there was a boy; he could not read you can simply just go to the library and get a maths book and start reading the content then you will start to understand the problems,” she added The Literacy Fair was met with widespread praise from parents who attended who has two children enrolled in the infant and grade-three programmes told JIS News that he welcomes the school’s focus on reading which he noted “widens their vocabulary and helps them to understand well” in acknowledging the role of parents in the teaching and learning process of their children recommended that parents introduce interactive reading in their homes to pique their children’s interest in reading “Kids at a young age are very imaginative so if [parents] can make the characters come alive [students] will be more engaged and have fun doing it… and will actually remember and learn from it,” he pointed out He reminded parents about the importance of engaging their children in reading from an early age for the majority of [people] in our society [who] don’t read very well it’s because from a tender age they weren’t put in the place or given the help to move forward in their reading,” Mr noting that the literacy fair aligns with the school’s long-term vision of enhancing academic achievement we would have recognised that many of our students are not reading at their grade levels and so we wanted to spark their interest in reading,” he said is for our students to be more interested in reading We want our students at this school to be reading at their grade levels We want them to be exiting grade six doing very well in terms of accessing the curriculum decode words and basically to become excellent leaders in society,” he told JIS News The school’s Literacy Committee will continue efforts to foster a reading culture and provide students with the tools they need to become confident and skilled readers Friday’s event was supported by several entities including Jamaica Information Service (JIS) Send An Email Get the latest news & updates from the JIS sent to your inbox Jamaica — Assistant Superintendent of the York Park Fire Station Courtney Thompson says two shops were completely destroyed during Monday morning’s fire at the Constant Spring Arcade in St Andrew He was providing the report to Observer Online shortly after firefighters performed cooling down operations at the location Thompson said firefighters were alerted to the blaze approximately 8:05 am and dispatched four units “Immediately we would have dispatched Half Way Tree Stony Hill and two units from York Park — based on the number of calls that we received and the severity of the arcade the fire was confined to three shops used as snack centres two of them would have completely destroyed and one partially destroyed,” Thompson said The cause of the fire is still unknown at this time but Thompson said investigators will carry out their duties in short order The Constant Spring Arcade is owned by the Kingston and St Andrew Municipal Corporation READ: WATCH: Fire at Constant Spring Arcade in St Andrew Copyright © 2025 Multimedia Jamaica Ltd Oshae Haye and Jodi Munn-Barrow copped the top spots at last weekend’s Constant Spring Golf Classic held at the Constant Spring Golf Course in St Haye competed in the WAGR (World Amateur Golf Ranking) section where the golfers complete three rounds or 54 holes in two days Golfers who compete in that section stand to gain WAGR points which allow them to play in tournaments across the region and the world Haye was the only golfer to post an under-par score which he did in the third round He posted two under par 68 and five over par 75 each in of the first two rounds for a total score of eight over par 218 His scorecard showed one birdie each in the first two rounds but had a very good third round which included four birdies He was three under par after 14 holes but bogeyed the next hole to close the round at two under par 68 who has not played in a local tournament for a couple of years was just one stroke behind Haye in second place after ending on 219 Knibbs posted the next best single round score of par 70 in his first round His other two rounds were three over par 73 and six over par 76 The normally reliable Zandre Roye had a decent first round of five over par 75 but a disastrous second round of 14 over par 84 His final round of one over par 73 contributed to his overall score of 22 over par 232 to be joint third with Dr Pro golfer Sebert Walker Jr was the only pro to finish that section of the Classic after posting scored of 77 president of the Jamaica Golf Association and perennial winner of local golf tournaments was again in the winners’ circle in the 0-12 section She scored eight over par 78 and three over par 73 for a total score of 13 over par 153 to carry the banner for the ladies "The Constant Spring Classic is the last qualifier for the JGA calendar which allows players points for national representation (I am) very pleased with the level of play that we saw The course was playing tough and Oshae came back and played very well on Sunday and was victorious” said Munn-Barrow Yvonne Brown took the 13-24 section after scoring 213 for her two rounds Vikram Dhiman was the number one player in the Men & Men Senior 0-6 The Men & Men Senior 7-12 went to Arshad Ali` who posted scores of 72 and 81 for a total score of 13 over par 153 while Bruce Levy (172) and Cedric Blair (177) were second and third respectively Jordan Wong 163 and Andrew Spencer 186 were first and second in the Men & Men Senior 13-24 section Mike Gliechman 164 topped the Men Super Senior 0-12 section while Guan Arscott 193 took the Men Super Senior 13-24 section and Philip Wilson 174 bagged the Masters category Jamaica — A section of the Constant Spring Arcade in St Andrew is on fire It is unclear at the moment what caused the fire but black smoke could be seen billowing from the building Monday morning At least one unit from the Jamaica Fire Brigade is now on the scene View the discussion thread. Constant Spring FC turned on the style on Saturday with a commanding 4-0 victory over Santos FC in the latest round of the KSAFA Championship League The home side asserted their dominance early and never looked back as they stormed to one of the biggest wins of the weekend The result was a major boost for Constant Spring as they continued their push for promotion while Santos were left to rue defensive lapses that were punished with ruthless efficiency Boys Town and JDF FC played out a cagey 0-0 draw in a match that lacked cutting edge in the final third Olympic Gardens FC edged Real Mona FC 2-1 in an entertaining affair with Cheanardo Anderson and Andre Leslie scoring for the hosts while Jordan Fowler pulled one back for Mona Duhaney Park FC secured a hard-fought 1-0 win over August Town FC thanks to a decisive strike from Akeem Brown Shortwood United also got the better of Browns Town FC 2-1 with goals from Malik Morris and Theodore Johnson Markland Gracey netted the consolation for Browns Town Barbican FC outgunned Maverley/Hughenden FC 4-2 Dwayne Barnes opened the scoring for Barbican before Akino Bailey added a brace Marverly/Hughenden got their goals from Omarion Barrett and Gervier Grant View the discussion thread. Police process the scene after a shooting at Constant Spring tax office on Thursday Jamaica -Students of the Abilities Foundation in Kingston are set to benefit from a state-of-the-art sickbay courtesy of the Kiwanis Club of Constant Spring (KCCS) will serve as a critical facility for the students providing them with much-needed healthcare support It was officially opened during a ceremony at the school in Kingston on Friday underscored the importance of partnership for building a better Jamaica It is a true adage and something which is represented here in this partnership,” he pointed out Charles Jr commended the KCCS for its work with the Abilities Foundation noting that their collaboration has been ongoing for more than two decades “When you see a partnership last that long it means that the parties in the partnership are not just dedicated but they have goals and that there is a mutual benefit This is something which we want to continue This is something that we have to celebrate,” he said The minister noted that the Kiwanis Club has been a long-standing beacon of excellence and service and has been instrumental in many acts of kindness that have contributed to Jamaica’s development He urged other stakeholders in Corporate Jamaica to get onboard with the Kiwanis Club and do their part to build a better nation advised that the sickbay was the club’s flagship project for 2024-25 She said the space will provide a much-needed area for students who are unwell “The project involved retrofitting a 20-foot container This involved insulation and panelling of the interior proper flooring to ensure cleanliness and safety modification of the entrance to include windows and building a ramp for accessibility,” the president detailed With the physical infrastructure completed Gilzeane said the club will be embarking on phase two of the project which will involve sourcing medical equipment medical beds and over-the-counter medication recruiting a full-time nurse and securing the services of a doctor on call which is an entity under the Ministry of Labour and Social Security provides specialised vocational education and training for young adults with disabilities Jamaica— The Kingston and St Andrew Municipal Corporation (KSAMC) said it will take further action against vendors at the Constant Spring Arcade who have not complied with attaining their own Jamaica Public Service (JPS) meter in his address to the councillors during the first KSAMC monthly meeting for the year stated that the corporation provides power to the Constant Spring Arcade however vendors have installed high powered appliances and illegal electrical connections which inflates the electricity bill of the KSAMC The mayor added that this action by the vendors have also increased the risk of fires “The KSAMC provided one outlet and one light bulb socket per shop and lighting for the common area many operators have exceeded this capacity by installing high-consumption appliances “This has not only inflated our electricity bills but also created fire hazards compounded by evidence of unauthorised electrical connections,” he added The mayor informed that preliminary investigations indicate that last December’s fire at the Constant Spring arcade was caused by an electrical short circuit READ: WATCH: Two shops completely destroyed in Constant Spring Arcade fire Mayor Swaby said considering these actions the KSAMC cannot allow the vendors to remain on its JPS meter He stated that the fees collected from for shops or space rentals fall significantly short of covering the expenses incurred by the corporation for waste disposal I led consultations with vendors of the arcade clearly outlining the urgent need for each operator to secure their own JPS meters This discussion…8 attended by JPS officials Another meeting was held in September which included the JCF adherence has been inadequate,” Swaby said “The KSAMC can no longer allow vendors to remain on its meter We have written to them giving them time to enter into contracts with the Jamaica Public Service We will now pursue further actions against those who are non-compliant,” he added The alternative line is the Police Emergency 119 number The Jamaica Constabulary Force says updates will follow The word literacy is defined as the ability to read speak and listen in a way that lets us communicate effectively and make sense of the world and according to the United Nations Educational Scientific and Cultural Organization (UNESCO) it empowers and liberates by expanding people’s capabilities the Constant Spring Primary and Infant School empowered their students from all ages and grade levels at their literacy fair… held under the theme “Unlock your Superpower: Read” School principal Shay Dillon highlighted that the administration through its reading committee saw it fit to host this literacy fair because it was identified that many students were reading below their grade level Jamaica – An unidentified man was shot and killed by men travelling on a motorcycle along Constant Spring Road near its intersection with Dunrobin Avenue while travelling in a Mercedes-Benz SUV on Sunday morning He was reportedly one of at least two occupants of what has been described as a Mercedes-Benz GLE He was rushed to hospital where he was pronounced dead according to information reaching Observer Online Traffic was diverted from the area as police investigators processed the crime scene Jamaica – The Constant Spring Tax Office in St Andrew closed early Thursday following a shooting incident at the location It is understood that two armed men travelling on motorcycles attempted to rob an elderly couple on the compound but a plain clothes police officer intervened resulting in the men fleeing the scene There were no reported injuries in the incident Jamaica— Two men have been charged in relation to the seizure of a firearm on Constant Spring Road in St Andrew on Monday St Catherine and 24-year-old Markeion Slater Both men have been charged with possession of prohibited weapon Reports from the Constant Spring Police are that about 12:12 am police were on a patrol when they signalled the driver of a Toyota Voxy motor car to stop The driver complied and the police team searched the motor car One Smith and Wesson firearm was reportedly found Gooden and Slater were arrested and later charged on Saturday August 17 after a question-and-answer session in the presence of their attorneys View the discussion thread. “Tons of college kids come out for trivia night,” Aaron Nafziger says on a recent Wednesday afternoon sitting bench-side against the wall of Constant Spring the bar on Main Street in the heart of downtown Goshen that has evolved into the epitome of a town that seems to have a welcome place for everybody “Our clientele is really diverse,” Nafziger says who have been coming here for a long time because it was a clean place with no smoking Opened in March 2006 with the intention of becoming another Goshen live music venue Constant Spring was bought by Nafziger in June 2013 call me a Goshen product” — with a business degree and four working years building up his résumé as a financial adviser has already one-upped his predecessor by bringing food to the forefront of Constant Spring’s business model a model that targets the masses instead of limiting the focus to the built-in college crowd from Goshen College that is largely underaged anyway it’s not really the business model,” Nafziger says of catering to the college students “We do better food with better ingredients With 18 different beers on tap and “probably 30 or 40” additional domestic and international bottled brews plus a full bar stocked with wine and liquor the business model calls for an emphasis on drawing a wide-ranging clientele as long as they’re above the legal age of 21 But it’s the food that brings a smile to Nafziger’s face when asked about howConstant Spring has evolved in the past two years under his ownership “We have the stage,” he says of the large stage for bands situated at the back end of the long “so it was intended to be a music venue and a bar It’s still chicken sandwiches and burgers and salads and pulled pork and whenever possible we get it from local sources but now I think we hang with anybody for real good quality food.” Just to show that Goshen isn’t all local burgers and chicken Constant Spring sets aside Wednesday nights for cuisine from around the world The International Oval Plate Special features a worldly dish for $12 each Wednesday and rotates among Asian pork tacos (the featured special on this particular Wednesday) Cuban pork sandwich and Korean beef barbecue Nafziger says he limited the Wednesday rotation to five dishes in an effort to sell familiarity to the customer I’ve never had that before and it sounds kind of good but I know the burger is good so I’m just going to get the burger.’ “We actually found that fewer people were getting it when they didn’t have the opportunity to try it two or three times you should really go get them tonight.’ So we’re at five (dishes) right now … on rotation I think it’s really fair in comparison to other places around.” which has turned into the popular week night for Goshen College kids to get out and unwind at Constant Spring “Tons of college kids come out for trivia night Goshen College here is like that … the kids like trivia.” upscale bar food with Wednesday night international cuisine specials to midnight Tuesdays through Thursdays and 4 p.m • For more information: Call 574-533-3302 or visit the website aconstantspring.com View the discussion thread. Jamaica —The Jamaica Constabulary Force (JCF) is advising members of the public that all vehicles held at the Constant Spring Police Station’s pound will be impounded or auctioned if they are not retrieved in two weeks The JCF is encouraging individuals who want to make a collection to contact Inspector Roswess at 8768390558 or Corporal Brown at 8767990808 or the Constant Spring police at 8769241421 The $172.7-million Hanover Justice Centre will serve as a model for the buildout of a network of these centres islandwide The contract for the centre was signed on Friday (October 4) at the Justice Ministry’s Constant Spring Road office in Kingston said the centre is the first such dedicated facility and is intended to administer justice services in settling disputes “We do have Justice centres in all 14 parishes We have used [miscellaneous spaces] to put up justice centres but we want some model justice centres,” he said Chuck said the facility is a critical aspect of the governance structure “Justice as a commodity is as important as health education or any other [social structure] because without justice We intend that access to justice should be easy and that when you get access to justice that justice should be delivered in a timely manner It is not only getting justice in the courts; it is also in simple disagreements we are hoping that citizens will make use of these justice centres and services that these justice centres will be able to offer,” he added will be outfitted with office spaces for the Ministry of Justice’s social justice services including Child Diversion and Restorative Justice Office spaces will also be provided for the Custos and for Mediation Work is scheduled to be completed in September 2025 Automotive rumbled into Super Value Town Centre on Constant Spring Road last Sunday to see what the fuss was about The Jamaica Classic Car Club (JCCC) hosted its lively social event showcasing over 70 gleaming classics that left onlookers speechless The parking lot transformed into a vibrant display of automotive history with curious shoppers stopping in their tracks to admire the impeccably maintained vehicles The impressive turnout rivalled their January event proving that not even the midday sun could dampen the public’s enthusiasm for these beauties “This was a better turnout than we expected,” said Naresh Potopsingh “The attendees made it a fantastic meet and the quality of classic cars on display was truly exceptional.” “It’s incredible to see the public connect with Jamaica’s history through these remarkable motorcars,” he remarked The show wasn’t over by closing time either with the most notable late entry being a head-turning Honda NSX a testament to the club’s dedication to keeping the passion for classic cars alive But the JCCC isn’t resting on its laurels They are already in high gear for their next event: a Father’s Day celebration at Hope Gardens featuring never-before-seen classics and owners from across the island Gearheads and families alike won’t want to miss this opportunity to step back in time and witness a true celebration of automotive heritage View the discussion thread. Constant Spring FC delivered a dominant performance on Saturday securing a 4-0 victory over Maverly Hughenden FC in Zone A of the KSAFA Wray and Nephew Championship League The standout player of the match was Javier Wilmott scoring all four goals to lead his team to an emphatic win Shortwood United FC edged out Boys’ Town FC in a thrilling 3-2 contest while Akeem Burton added another to give Shortwood the win Olympic Gardens FC comfortably defeated Duhaney Park FC 3-0 with Zenard Street netting twice and Mario Burton also getting on the scoresheet JDF FC secured a narrow 1-0 victory over Santos FC Browns Town FC claimed a 2-1 victory over Real Mona FC with Carl Campbell and Jevaun Shepherd finding the back of the net Barbican FC and August Town FC played out a goalless 0-0 draw the competition in the KSAFA Championship League continues to heat up as teams battle for top spots in their respective zones Metrics details To describe fetal management of homozygous hemoglobin constant spring (Hb CS) Six fetuses with homozygous Hb CS disease undergoing intrauterine transfusion (IUT) were comprehensively reviewed when combined with 8 cases previously reported The first clues of diagnosis were hydropic changes suggesting fetal anemia Increased cardiothoracic diameter ratio (CTR) was the most sensitive sonographic marker but slowly changed after IUT whereas MCA-PSV was the most sensitive in response to IUT Gestational age at diagnosis was 17–29 (22.8 ± 3.3) weeks Rates of adverse obstetric outcomes were relatively high; preterm birth: 35.7% All showed good response to IUT with disappearance of hydropic signs and all survived without short-term complications Their anemia gradually improved in childhood and transfusion independent Homozygous Hb CS can cause severe fetal anemia Early diagnosis and IUT can improve neonatal outcomes probably preventing adult diseases caused by fetal programming Prices may be subject to local taxes which are calculated during checkout The distribution of the Hb constant spring gene in Southeast Asian populations Hemoglobin constant spring among Southeast Asian Populations: haplotypic heterogeneities and phylogenetic analysis Anemia and hydrops in a fetus with homozygous hemoglobin constant spring Fetal anemia causing hydrops fetalis from an alpha-globin variant: homozygous hemoglobin constant spring Fetal anemia and hydrops fetalis associated with homozygous Hb constant spring (HBA2: c.427T > C) The fetal origin of adult disease: from sceptic to convert Heart disease link to fetal hypoxia and oxidative stress Fetal origin of childhood disease: intrauterine growth restriction in term infants and risk for hypertension at 6 years of age Early-life origin of adult disease: evidence from natural experiments Sonographic markers of hemoglobin Bart disease at midpregnancy J Ultrasound Med: Off J Am Inst Ultrasound Med Download references The Journal Club is a collaboration between the American Academy of Pediatrics—Section of Neonatal Perinatal Medicine and the International Society for Evidence-Based Neonatology (EBNEO.org) This study was financially supported by Research Fund The authors declare that they have no conflict of interest This study was conducted in accordance with the principles of the Helsinki Declaration and obtained approval from the institutional review board of the Ethics Committee Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41372-019-0397-7 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article View the discussion thread. The National Water Commission (NWC) has programmed $1.8 billion for the rehabilitation and upgrading of wastewater treatment facilities This is part of multibillion-dollar investments aimed at rectifying aging pipelines and integrating renewable energy solutions as part of efforts to modernise Jamaica’s water infrastructure strengthen the entity’s resilience and reduce inefficiencies Details are outlined in the Jamaica Public Bodies Estimates of Revenue and Expenditure for the Year ending March 2026 A key programmed activity is the Western Resilience Pipeline Project designed to enhance service reliability by replacing aging transmission mains This project will see the installation of 29 kilometres of pipelines from Martha Brae to Montego Bay and replacement of the existing 250mm pipeline from Lucea to Negril It is expected to be executed over 18 to 24 months Additional expenditure is also earmarked to upgrade 7.2 kilometres of water mains from Ferry to Rock Pond in Red Hills as well as pipeline replacements along Washington Boulevard The NWC will also continue water system upgrading islandwide with an earmarked $1.03 billion Among the systems being targeted are Greater Mandeville $216 million will be used for installing new and more accurate meters Energy efficiency remains a central focus for the NWC with plans to reduce energy costs through the installation of solar systems It is anticipated that work will be completed by the fourth quarter of fiscal year 2025/26 at the Leader Avenue pump station (350kW) and Frasers Content pumping station (100kW) These initiatives are intended to reduce energy consumption from the national grid by 800kW during the daytime The NWC will also focus on reducing non-revenue water (NRW) particularly through the co-management programme in Portmore This initiative aims to cut NRW levels from 21,000 to 10,500 cubic metres per day Established in 1980 through a merger of the Kingston and St Andrew Water Commission and the National Water Authority the NWC currently supplies potable water to more than 74 per cent of the population and provides sewerage services to 19 per cent The Commission manages an extensive network of more than 1,000 water supply facilities 11,000 kilometres of water mains and over 68 wastewater treatment plants Tax Administration Jamaica (TAJ) will open the doors of select Tax Offices this Saturday as it continues to provide weekend services every last Saturday of the month for the 2024/25 financial year The following Tax Offices will operate this Saturday between the hours of 10:00 am – 2:00 pm:  Montego Bay (FCJ Building at 11-12 Almond Way The Portmore Tax Office will continue its usual weekly Saturday operations with adjusted business hours (9:00 am – 2:00 pm) The Tax Authority continues to invest in its month-end Saturday operations thereby alleviating the usually high walk-in traffic at Tax Offices during the busy month-end weekday period Taxpayers have welcomed the enhanced service feature which gives them the convenient option of doing their usual business on the weekend These services includes access to taxpayer services such as same day driver’s licence processing motor vehicle registration and other motor vehicle transactions but excludes those of Compliance and Audit transactions Persons have the option to also apply for a Taxpayer Registration Number (TRN) as the Tax Authority continues to provide additional access to its services Individuals are able to do business on the go or in the comfort of their homes by conducting several transactions with the Tax Authority online which helps them to avoid visiting a Tax Office These transactions take into consideration Motor Vehicle Registration Certificate (MVRC) renewal online as well as electronically querying Property Tax liabilities Customers may also avail themselves of a range of online payment options via the TAJ Direct Funds Transfer service for business-related tax payments These are in addition to customers being able to make payments via the Tax Authority’s website www.jamaicatax.gov.jm using a credit card or other banking card with credit card features taxpayers have the option to utilize both the National Commercial Bank and Scotiabank Online banking and make tax payments via their online banking platforms by adding TAJ as a Payee The Tax Authority also offers its expanded Direct Funds Transfer (DFT) payment channel which includes Stamp Duty and Transfer Tax as a payment type as well as its Scotiabank account as a beneficiary account This means that in addition to the ability to make payments for Payroll Deductions as well as several other business-related taxes customers now also have the ability to make Stamp Duty and Transfer Tax payments using the convenient DFT option For further information call the Tax Administration Customer Care Centre at 888-TAX-HELP (888-829-4357) or visit the website www.jamaicatax.gov.jm. Metrics details An Author Correction to this article was published on 10 November 2021 This article has been updated The thalassemia of Hemoglobin H-Constant Spring disease (HbH-CS) is the most common type of Thalassemia in non-transfusion thalassemia the clinical manifestations of the same genotype of thalassemia can be vastly different we used microarray technology to reveal the epigenetic regulation of m6A in modifiable diseases and demonstrated a role of BCL2A1 in disease regulation we revealed that methylating enzyme writers including METTL16 and ZC3H13 displayed low expression and the demethylating enzyme ALKBH5 along with reader proteins including IGF2BP2 and YTHDF3 exhibited high expression BCL2A1 was hypo-methylated and showed low expression We also revealed that the BCL2A1 methylation level and IGF2BP2 expression were negatively correlated the mRNAs expression between ALKBH5 and IGF2BP2 were positively correlated which may play an important role in the process of red blood cell differentiation and development of HbH-CS the mRNA-M6A methylation status may be regulated by the demethylating enzyme ALKBH5 via IGF2BP2 the effect of epigenetic modification on HbH-CS must be considered we analyzed the link between epigenetics and the HbH-CS phenotype using the human m6A-mRNA and lncRNA epitranscriptomic microarray data from the immature erythrocytes of 5 HbH-CS thalassemia (T) and 5 normal healthy volunteers (N) Our goal was to elucidate the underlying mechanism behind HbH-CS pathogenesis Confirmation of red blood cell sorting, using flow cytometry. Aggregation of signals in the Q3 region is indicative of a mostly (90.2%) CD71+ (denoting RBC) cell population. Expression and methylation profiles of mRNAs in immature RBCs of Hb cs thalassemia (T) and healthy volunteers (N) The differentially expressed profile of m6A-mRNAs in immature red blood cells of Hb CS thalassemia (T) and healthy volunteers controls (N) (*P < 0.05.) The qRT-PCR data (A) was consistent with the epitranscriptomic rnicroarray sequence (B) The top ten enrichment scores of significant enrichment hypo-methylated genes ontology (in bar format) depicted genes from the biological process Significance was established at p-value 0.05 The top ten enrichment scores of significant enrichment hypo-methylated genes pathway (− log10 (Pvalue)) (B) The degree of red corresponds to significance The size of the dots corresponds to mRNA entities that are directly linked to the noted Pathway IDs The “m6A methylation level” for all transcripts was calculated as the percentage of Modified RNA (% Modified) in all RNAs based on the immunoprecipitated (IP) (Cy5-labelled) and supernatant (Sup) (Cy3-labelled) normalized intensities: % Modified = (modified RNA)/(Total RNA) = IP/ (IP + Sup) = 〖IP〗_(Cy5 normalized intensity)/(〖IP〗_(Cy5 normalized intensity) + 〖Sup〗_(Cy3 normalized intensity) ) BCL2A1 mRNA levels are modulated by m6A associations. (A) An illustration of m6A sites in BCL2A121 (http://m6avar.renlab.org/) (B) The m6A-BCL2A1 association was severely hypo-regulated (P < 0.0001) (C) BCL2A1 transcription was markedly reduced in T versus N (P = 0.0085) (D) BCL2A1 methylation status and IGF2BP2 levels were negatively correlated (r = − 0.7015 (E) Transcriptional relationship between ALKBH5 and IGF2BP2 (r = 0.6989 P-values were derived from Student's t-test and Correlation Analysis ALKBH5 and BCL2A1 in K562 transfected with siRNA negative control (siCtrl) ALKBH5 and BCL2A1 in K562 transfected with siRNA negative control (siCtr1) To determine whether m6A modification is involved in producing the thalassemia phenotype we assessed immature erythrocytes from the peripheral blood of 15 T and 16 N We identified that methylating enzymes like METTL16 and ZC3H13 were scarcely expressed and the demethylating enzyme ALKBH5 we detected differential regulation of other m6A-methylated RNAs like RBM15 we believe that the m6A-mediated methylation is crucial for the pathogenesis of HbH-CS When BCL2A1 levels diminish during erythroid development we detected BCL2A1 in 3 separate signaling pathways Acute Myeloid Leukemia and transcriptional misregulation in cancer (Supplemental Information) we also discovered that the m6A methylation status of BCL2A1 was negatively correlated with IGF2BP2 gene expression m6A methylation was not directly affected by the M6A-associated enzymes ALKBH5 is positively correlated with IGF2BP2 we propose that ALKBH5 regulates RBC differentiation and development by altering the methylation status of BCL2A1 via IGF2BP2 this requires further study and confirmation M6A plays a significant role in thalassemia explored the m6A RNA methylation status and its regulation of RNA stability in HbH-CS patients we established a profile of differentially methylated mRNA in T versus N samples we proposed involvement of m6A-regulated BCL2A1 in the pathogenesis of thalassemia more investigation is necessary to explore the methylation status of relevant RNAs and modulation of target downstream genes during erythroid differentiation we discovered that there were two m6A methylation sites in BCL2A1 we did not elucidate which site holds more significance to thalassemia the underlying mechanism behind RNA methylation of BCL2A1 was not examined The sorted RBC samples were subsequently maintained in TRIZOL at − 80 °C for further analysis Total RNA was extracted with RNAiso Plus (Takara) and cDNA was synthesized with Prime Script TMRT reagent Kit with GDNA Eraser (Perfect Real-Time; Takara Bio real time polymerase chain reaction (RT-qPCR) was conducted with the QuantiNova SYBR Green PCR Kit (QIAGEN β-Actin was employed for normalization of gene expression 1–3 μg of total RNA and m6A spike-in control were introduced to 300 μL 1 × IP buffer (50 mM Tris–HCl 40U/μL RNase Inhibitor) with 2 μg of anti-m6A rabbit polyclonal Ab (Synaptic Systems) The solution was then maintained with head-over-tail rotation at 4 °C for 2 h 20μL (per sample) of Dynabeads™ with M-280 Sheep Anti-Rabbit IgG suspension was blocked with freshly made 0.5% BSA at 4 °C for 2 h and resuspended in the total RNA-antibody mixture described above RNA was allowed to bind to the m6A-Ab beads during head-over-tail rotations at 4 °C for 2 h the beads were rinsed thrice with 500 μL 1 × IP buffer and twice with 500 μL wash buffer (50 mM Tris–HCl the enriched RNA was eluted with 200 μL elution buffer (10 mM Tris–HCl before extraction and precipitation with acid phenol–chloroform and ethanol The modified RNAs were eluted from the immunoprecipitated magnetic beads as “IP” (immunoprecipitated The unmodified RNAs were recovered from the supernatant as “Sup” (supernatant The “IP” and “Sup” RNAs were then labeled with Cy5 and Cy3 respectively The RNAs were combined together and hybridized onto Arraystar Human mRNA & lncRNA Epitranscriptomic Microarray (8 × 60 K the arrays were scanned in the two-color channels by an Agilent Scanner G2505C K562 cell lines (Procell Biotechnology Co. CHINA) were cultured as grown in RPMI medium 1640 basic (Gibco) supplemented with 10% fetal bovine serum (FBS) (Sijiqing Cells were transfected with siRNAs (final concentration: 20 nM) by riboFECT™ CP Reagent (RiboBio China) according to the manufacturer’s instructions All siRNAs were obtained from Tsingke Biotechnology Co.Ltd China) shown below: IGF2BP2 sense: GAAGUGAUCGUCAGAAUUATT antisense: UAAUUCUGACGAUCACUUCTT; siRNA Negative control (siCtrl) IGF2BP2、ALKBH5 and BCL2A1 expression were confirmed by RT-Qpcr and Immunoblot Cells were lysed using in RIPA buffer (Beyotime Proteins were electrophoretically resolved on 10% or 15% SDS–polyacrylamide gels (40 μg per lane) and transferred onto nitrocellulose membranes (Bio-Rad and electroblotted onto polyvinylidene difluoride (PVDF) membranes After blocking with 5% BSA Blocking Buffer (Solarbio China)The membranes were washed 3 times with TBST (Solarbio After incubation with appropriate first antibody 4 °C overnight and HRP-coupled secondary antibodies at room teperature for 1 h Target proteins were detected in Gel and chemiluminescence dual imaging system (FluouChem HD2 CA USA)and developed with BeyoECL Plus (Beyotime China) and images analyzed with ImageJ (version 1.51j) Antibodies used for experiment were as follows: GAPDH (60004-1-1 g 1:2000) and HRP-coupled secondary antibodies (SA00001-1,1:2000) were from Proteintech Group BCL2A1 (A0134,1:500) antibody was from Abclonal (Wuhan The Agilent Feature Extraction software (version 11.0.1.1) was employed for the analysis of acquired array images Raw intensities of Modified RNA (Cy5-labelled) and Unmodified RNA (Cy3-labelled) were normalized with the mean of log2-scaled Spike-in RNA intensities signals with Present (P) or Marginal (M) QC flags in a minimum of 1 in 10 samples were marked as “All Targets Value” in Excel for further “m6A methylation level” “m6A quantity” and “expression level” calculations The “m6A methylation level” was measured via the percentage of modification based on sample normalized intensities The “m6A quantity” was assessed from the Modified RNA (Cy5-labelled) normalized intensities The “RNA expression level” was analyzed according to the normalized intensities of all RNA we performed additional quartile normalization using the limma package to ascertain array expression Differentially m6A-methylated RNAs or differentially expressed RNAs between the HbH-CS and healthy volunteers were recognized by fold change and statistical significance (p-value) values hierarchical clustering was done to show degrees of m6A-methylation within samples Our works received ethical approval from the First Affiliated Hospital of Guangxi Medical University All participants agreed to sign informed consent forms All authors agree to the publication of this article The following information was supplied regarding data availability The sequencing data are available in the Supplemental Files A Correction to this paper has been published: https://doi.org/10.1038/s41598-021-01793-3 Beta­thalassemia: From genotype to phenotype Guidelines for the management of non transfusion dependent thalassaemia (NTDT) (Thalassaemia International Federation The definition and epidemiology of non-transfusion-dependent thalassemia The inherited diseases of hemoglobin are an emerging global health burden Global epidemiology of haemoglobin disorders and derived service indicators Heterogeneity of hemoglobin H disease in childhood Epigenetic inactivation of ERF reactivates γ-globin expression in β-thalassemia A natural DNMT1 mutation elevates the fetal hemoglobin level via epigenetic derepression of the γ-globin gene in β-thalassemia Polycystic ovary syndrome is transmitted via a transgenerational epigenetic process Genome-wide impact of hydrogen peroxide onmaintenance DNA methylation in replicating cells The histone mark H3K36me2 recruits DNMT3A and shapes the intergenic DNA methylation landscape m6A-RNA demethylase FTO inhibitors impair self-renewal in glioblastoma stem cells FTO-dependent N6-methyladenosine modifications inhibit ovarian cancer stem cell self-renewal by blocking cAMP signaling Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia m6AVar: A database of functional variants involved in m6A modification Chemical genetic straregy identifies histone deadetulase1 (HDAC1) and HDAC2 as therapeutic targets in sickle cell disease MicroRNAs in erythroid and megakaruocytic differentiation and megakaryocyte-erythroid progenitor lineage commitment Mir-q44 selectively regulates embryonic alpha-hemoglobin synthesis during primitive erythropoiesis A molecular study on the role of alpha-hemoglobin-stabilizingprotein in hemoglobin H disease Dynamic transcriptomic m6 A decoration: Writers RNA modifications modulate gene expression during development Dynamic transcriptomic m(6)A decoration: Writers TRIM17 and TRIM28 antagonistically regulate the ubiquitination and anti-apoptotic activity of BCL2A1 MiR-140-5p inhibits cell proliferation and metastasis by regulating MUC1 via BCL2A1/MAPK pathway in triple negative breast cancer Recognition of RNA N6 -methyladenosine by IGF2BP proteins enhances mRNA stability and translation Dynamic m6 A mRNA methylation directs translational control of heat shock response GATA-1 and erythropoietin cooperatr to promote erythroid cell survival by regulating bcl-Xl expression Revisiting the non-transfusion-dependent (NTDT) vs transfusion-dependent (TDT) thalassemia classification 10 years later Management of non-transfusion-dependent β-thalassemia (NTDT): The next 5 years A DNA/Ki67-based flow cytometry assay for cell cycle analysis of antigen-specific CD8 T cells in vaccinated mice Hematopoietic stem cell cultures and assays Download references We are grateful to Professor Lihong Pang for her helpful suggestions and thank doctors Fang Yang and Xueyu Li for their help in collecting clinical data The views expressed in this article are the personal opinions of the authors and do not reflect the policy of the Department of Health and Human Services Funding This work was supported by grants from the National Natural Science Foundation of China (Nos Special Fund of Characteristic Innovation Team of the First Affiliated Hospital of Guangxi Medical University (NO YYZS202008) and the construction of clinical intervention protocols Guangxi key R & D program (Guike AB20159031) The First Affiliated Hospital of Guangxi Medical University Affiliated Minzu Hospital of Guangxi Medical University is a major participant in this study and the author of the paper; F.Y. participated in the collection of specimens for this topic and the analysis of subject data participated in the design of the topic and overall experimental guidance The authors declare no competing interests Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations The original online version of this Article was revised: The original version of this Article contained an error under subheading “Participants and samples” “Patient demographics are provided in Table 4.” now reads: “Patient demographics are provided in Table 1." Download citation DOI: https://doi.org/10.1038/s41598-021-99867-9 Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology President of the Kingston and St Andrew Football Association (KSAFA) said the football fraternity has lost a mentor and father figure with the passing of Constant Spring Football Club founder Maurice ‘Danny’ Lyn who passed away yesterday morning in Canada was residing with family overseas at the time of his passing and Shaw said the former coach and administrator will be missed He is someone you can always go to for assistance and he has done so much to help a lot of young men and women,” Shaw said “When we (KSAFA) use his football complex but Danny is a lover of football and KSAFA so he had no issue [in] letting us use it His contribution was invaluable and he will truly be missed,” Shaw added “It is a great loss to football in Jamaica and I just want to say to his family and friends Danny’s brother and former Constant Spring team manager Danny spent the last four months in Canada getting treatment “He has been ailing seriously for about four months He went to Canada to try and see what they could have done there but there was only so much that they could do as he was deteriorating as the days go by,” he revealed Neville recalled how his bother built the club from the ground up with mainly community players from in and around the area and stamped it with his own personal style of play “He started the club with just players from the Cassava Piece area and developed a pattern of football for himself and took it straight to the Premier League “It (club) was more about family and helping youths in the area It wasn’t just about football for him he wanted to build better characters and better people.” “The complex is also his legacy and I will try my best to see what I can do to maintain that complex He built a legacy with the football team and whoever is there to take care of it I will always be there to assist Constant Spring football I will not allow it to die; we have to keep his legacy alive,” he added He is survived by his wife and seven children (four boys and three girls) hailed Lyn as a stalwart of Jamaican football “The Jamaica Football Federation mourns today as football has lost a stalwart gave his heart and his life to the sport he loved although coaching and mentoring were his passion Danny contributed to the success and livelihood of countless youngsters over many years The Constant Spring Football Club and the Constant Spring football field stand as monuments to his support of the beautiful game,” read the release livingston.scott@gleanerjm.com View the discussion thread. View the discussion thread. CELEBRATION filled the air at Constant Spring Primary and Junior High School on Monday as the institution observed its 50th anniversary and the start of the Easter term according to Acting Principal Michelle Wiggins was justified as in her estimation the school had come a far way and staff was grateful to see its growth the entrance was decorated with balloons of the school’s signature colours — yellow and blue — and a red carpet which students and staff graced as they received a high five from the Minister of Education and Youth Fayval Williams to the song Jerusalema by Master KG sharing a memorable moment throughout her 31 years of service to the institution noted that the literacy improvement was a major accomplishment She said when she became principal in 2010 the literacy rate for grade four students moved from about 35 per cent to 86 per cent “I have been here from 1987 and when I became principal in 2010 and realised that the literacy rate was about 35 per cent I asked the teachers of grade four to give some time to teaching the students both in maths and in language arts and I taught even as principal,” she said it was published in the papers and we got 86 per cent from about 35 per cent and I think that was really a memorable moment because together by giving a little extra time to teaching,” she told the Jamaica Observer Minister of Education and Youth Fayval Williams who commended the school on their milestone also encouraged students to obey the school rules and maintain COVID-19 protocols “This school serves communities that are vulnerable communities; communities in which tough things happen sometimes and for it to be here thriving and surviving it is the school of choice in the area and that is something to celebrate,” she said I am expecting that you will be following in the footsteps of those who graduated gone on to college to become leaders in Jamaica leaders in other parts of the world,” added Williams We have been hearing more news stories about COVID-19 all over the world and I just want to encourage you students to remember what you have learnt about hand washing at school and home so that we are able to continue school in the face-to face mode,” she said past and present leaders of the institution expressed gratitude for the school’s performance over the years while predicting more growth and achievements “We continue to persevere for excellence We have excellent teachers at this institution brilliant students and of course we continue to blaze the trail of excellence,” said the school’s Principal Shay Dillon during the back-to-school welcome devotion The school will engage in a church service on January 28 followed by other events such as a banquet and open day for its 50th anniversary celebration the Constant Spring Primary and Junior High School was established in 1973 View the discussion thread.