Nearly 200 emergency nurses take part in association's annual Day on the Hill 2025 /PRNewswire/ -- Emergency nurses from across the country took to the nation's capital on Wednesday to emphasize the need for passage of legislation focused on supporting their health As part of the Emergency Nurses Association's annual Day on the Hill event nearly 200 members participated in dozens of meetings with members of Congress and their staffers to highlight two key bills – the Save Healthcare Workers Act and the Dr Lorna Breen Health Care Provider Protection Reauthorization Act – which prioritize action to mitigate the epidemic of workplace violence in emergency departments and provide funding for programs which help health care workers with their mental health and well-being needs ENA members have used their voices on Capitol Hill as part of this cornerstone ENA event to bring awareness and call for action on the issues of greatest importance to emergency nurses their peers and their patients," said ENA President Ryan Oglesby "Our members take great pride in being fierce advocates particularly when it comes to protecting health care workers from violence on the job and ensuring there are resources available for those coping with job-related stress and burnout." With acts of verbal and physical violence looming over every emergency department ENA members sought legislator support for making the assault of a health care worker a federal offense as outlined in the Save Healthcare Workers Act which is poised for reintroduction soon Lorna Breen Act's initial journey through Congress as it was designed to create funding for programs and resources nurses physicians and others in health care amid the strain of the COVID-19 pandemic ENA's advocates urged for this important funding to continue as concerns persist about emergency nurses leaving their jobs because of the personal and professional toll on their health Learn more about ENA's legislative priorities and the association's actions by visiting ena.org/advocacy ENA Media Contact:Dan CampanaDirector of Communications847.460.4017[email protected] Dedicated to reaching emergency nurses worldwide the Emergency Nurses Association was recently granted specialist affiliate status by the.. When nurses have in-depth knowledge to manage complex trauma cases and can anticipate injury patterns with the appropriate interventions Health Care & Hospitals Medical Pharmaceuticals Advocacy Group Opinion Not For Profit Do not sell or share my personal information: This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page Please select what you would like included for printing: Copy the text below and then paste that into your favorite email application 2025 in the presence of her husband Foua Hang at their home in Lake Elmo her yorkie Pixie; mother Darling; siblings Mollie (Mai) (Wa MengVang) Lena (Kendrid); nieces Abbigail “Yoyo” and Mailea; along with many loving cousins and their children Ena is preceded in death by father Doua Her Ena was a firecracker - knowledgeable in all aspects of life Her presence is known when she enters a room and it is felt when she is not in attendance Ena faced many adversities in life but she did not allow it to stop her from dreaming She loved hard on her people and is the epitome of resilience in the face of adversity Her unexpected death is felt by many that she touched.  Please join her family in celebrating a beautiful life lost too soon on Sunday April 20th 2025 11 AM to 7 PM at Bradshaw Celebration of Life Center Bradshaw Celebration of Life Center - Stillwater Enter your phone number above to have directions sent via text This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply Service map data © OpenStreetMap contributors new trauma course strengthens ED nurse expertise 2025 /PRNewswire/ -- When nurses have in-depth knowledge to manage complex trauma cases and can anticipate injury patterns with the appropriate interventions they are more confident when caring for patients with severe injuries The Emergency Nurses Association recently launched the Trauma Nursing Advanced Course designed to equip nurses with the critical thinking and additional knowledge they need to strengthen their expertise trauma and critical care nurses already experienced in trauma care who are seeking advanced knowledge in trauma management severe injuries in the ED can require an advanced level of care," said ENA President Ryan Oglesby "TNAC is able to provide experienced trauma care nurses with the higher-level critical thinking and clinical skills necessary to take care of complex trauma patients with skill nurses must have a current Trauma Nursing Core Course verification after taking an 8th or 9th edition course Nurses completing TNAC receive 25 contact hours and a four-year verification demonstrating expertise in advanced trauma care with online modules; in-person or virtual classroom sessions; and a final in-person simulation experience The course covers a wide range of advanced trauma topics including critical thinking and clinical decision-making massive transfusion and hemodynamic management A study done by ENA researchers identified elements of an advanced trauma nursing program for the emergency department and defined advanced trauma nursing practice education curriculum for emergency department nurses The results of this study served as the foundation for the content to be included in TNAC "ENA is proud to have a team of researchers dedicated to improving emergency nursing care and education for nurses so they can provide the best patient care possible," said Oglesby "Watching TNAC come to fruition has been exciting as ENA is the only nursing association that develops and delivers its own research." To learn more about TNAC or bringing it to your hospital, click here ENA Media Contact:Morgan WietechaMedia Relations Strategist847.460.4038morgan.wietecha@ena.org View original content to download multimedia:https://www.prnewswire.com/news-releases/ena-launches-new-trauma-nursing-advanced-course-302426036.html We’d like to share more about how we work and what drives our day-to-day business How we use your information depends on the product and service that you use and your relationship with us To learn more about how we handle and protect your data, visit our privacy center Read our editorial policy to learn more about our process Monitor weather reports before your park visit •[MAUI] UPDATE 5/2/25 - Polipoli Spring State Recreation Area has REOPENED 8:00 AM to 4:00 PM Hawaii Standard Time (HST) except Federal and State Holidays [MAUI] - UPDATE 4/17/25: Puaʻa kaʻa Wayside and Restrooms will be CLOSED for renovations April 21 thru May 12 [MOLOKA’I] - UPDATE 3/24/25: Pala’au State Park Pavilion: Due to demolition and renovation work the pavilion will be closed from March 25 to May 31 [KAUAʻI] - UPDATE 3/21/25: Pā‘ula‘ula State Historic Site - Due to construction improvements the park closure will begin starting March 24 until further notice [OʻAHU] UPDATED 3/18/25 – Diamond Head State Monument (DHSM): Partial Park Closures Hours with operating hours from 6:00 AM to 2:00 PM will occur from April 21 onwards Monday through Friday Normal Park Hours from 6:00 AM to 6:00 PM will resume on Weekends and Holidays During Partial Park Closures Hours from 6:00 AM to 2:00 PM the last reservation time slot for Parking and Entry and Entry Only will be 12:00 p.m you must enter the Park no later than 12:30 p.m upon completion of the rock fall mitigation construction project at the Kahala Tunnel The Vehicle Access Special Use Permit is required to drive a vehicle into Ka‘ena Point State Park Reserve beyond the gate and the paved road that ends at the gravel parking area Initiated in 2015 primarily as a cultural and natural resources management tool that was generally supported by the community to protect Ka‘ena this permit system aimed to curtail 20 years of increasing landscape degradation caused by uncontrolled four-wheel drive vehicle use in the reserve Permit conditions require drivers to remain on a limited number of designated dirt roads and stress that this is not a four-wheel drive recreation area – but that the designated roads are for responsible access for fishing coastal access and to get to the Natural Area Reserve and the point Step 1: Call the Gate Combination Interactive Voice Response (IVR) system at (808) 207-9488 Step 2: Enter your assigned Confirmation Number when asked for your Confirmation Number New permit applications are not being accepted at this time Please check back here at the end of the year  If you need to contact Customer Service or Technical assistance, email [email protected] or call (808) 695-4620 Contact State Parks for other inquiries about the Kaʻena Point State Park, email [email protected] or call (808) 587-0300 The Kaʻena Point Vehicle Access Permit allows vehicles to access designated roads in the permitted section provided on the KP-Designated-Road-Map-2024. Please stay on designated roads. Park Rules & Permit Special Conditions The royals were out in full force at the Formula One race this weekend, with Harriet Sperling joining her new beau's cousins and aunt ahead of the race Lady Spencer of Alresford and the Marquess of Milford Haven have become grandparents once again, as their daughter, Lady Tatiana Mountbatten, welcomes a second child with her husband, Alexander ‘Alick’ Dru (standing left to right) Princess Helena Victoria of Schleswig-Holstein Duke of York; (seated left to right) Princess Victoria Eugenie of Battenberg Duchess of York holding Prince Edward of York Prince Arthur of Connaught (hand on chin) and Prince Alexander of Battenberg before her wedding to King Alfonso – he noticed her because of her nearly white platinum hair The Princess Royal’s son is the director of a maritime firm, which many have interpreted as a nod to his mother's love of sailing But it was at Buckingham Palace that the next chapter in Victoria Eugenie’s life would unfold King Alfonso XIII of Spain was on an official visit to meet King Edward VII and the palace was holding a banquet in his honour The King was one of Europe’s most eligible bachelors with much of the court assuming that he would marry Victoria Eugenie’s cousin as during the state dinner King Alfonso asked his hosts the name of the girl with the almost white hair Despite sitting between Queen Alexandra and Princess Helena the King of Spain had been taken by Victoria Eugenie – and her platinum blonde tresses Throughout their intercontinental courtship it soon became clear that Alfonso’s mother she did not consider the Battenbergs to be royal and Maria Cristina feared introducing the genetic disease into the Spanish royal bloodline The first two of the Queen’s complaints were dealt with (Victoria Eugenie agreed to convert and was shipped off to Versailles to receive instruction in the Catholic faith) but the third would haunt her family for the rest of Ena’s days On 13 May 1906, an estimated 400,000 people gathered to watch the royal procession outside the Church of Saint Jerome the Royal. Spain’s most aristocratic houses attended, with godparents including Infante Carlos of Bourbon-Two Sicilies. The Minister of Grace and Justice registered the marriage in an official register, and, with that, Victoria Eugenie became the Queen of Spain. A portrait of Queen Victoria Eugenie of Spain from 1927 Crown Prince Hussein and Princess Rajwa welcomed Princess Iman on 3 August 2024 Queen Victoria Eugenie of Spain arrives in London for the wedding of Princess Alexandra Despite her troubled standing and torrid home life, Queen Victoria Eugenie dedicated her life to uplifting the poor. Her work with the Red Cross was honoured in 1929, when the city of Barcelona erected a statue of her in a nurse’s uniform When a swathe of republican sentiment led to the declaration of the Second Spanish Republic in 1931 She would go on to split her time between the UK and Viellie Fontaine Ousted from Italy – due to her affiliations with the Allied Powers in the midst of World War Two – Victoria Eugenie returned to Spain in 1968 for the baptism of her great-grandson who would go on to become King Felipe VI of Spain The once Queen of Spain died in Switzerland on 15 April 1969 at the age of 81 It would be 16 years until her remains were re-interred in the Spanish Royal Vaults Queen Victoria tells the young royal: ‘Do not cry nobody should know about your emotions.’ In the coming months surely many will know her story for the first time MELBOURNE, Australia, April 09, 2025 (GLOBE NEWSWIRE) -- ENA Respiratory a clinical-stage pharmaceutical company developing antiviral host defence enhancers to minimize the impact of viral respiratory infections announces today that it has secured the University of Maryland’s renowned global health institution as clinical partner to conduct a Phase 2 proof of concept study of INNA-051 Professor of Medicine at the University of Maryland School of Medicine’s Center for Vaccine and Global Development (CVD) and a global health physician scientist with expertise in respiratory virus protection and control will be Principal Investigator INNA-051 is being developed as a convenient once-a-week nasal dry powder product to reduce the impact of viral respiratory infections and prevent severe complications in at-risk populations those with an underlying medical condition and individuals with occupational risk such first responders study of INNA-051 in generally healthy adult participants who are at increased risk to viral respiratory infections during the North American 2025/26 fall/winter season “Partnering with CVD and having Professor Justin Ortiz as one of the Principal Investigators for this Phase 2 trial is a significant step for ENA Respiratory,” said Christophe Demaison “The esteemed team at the University of Maryland School of Medicine led by Professor Justin Ortiz alongside colleagues Professor Kirsten Lyke and Dr brings extensive experience and a proven track record in conducting similar trials We now look forward to working together to deliver the next step in the development of what we believe could be a significant new approach to reducing the enormous burden of respiratory viral diseases.” said: “Viral respiratory infections are a major global public health problem and there is a clear need for new approaches to tackle the complications that lead to significant morbidity and mortality We are excited to be partnering with ENA Respiratory on this important study to test their new approach for boosting the body’s natural host defence pathways.” Ortiz previously served as a paid scientific advisor for ENA Respiratory but he no longer maintains any financial relationship beyond funding for research ENA Respiratory is a clinical-stage pharmaceutical company tackling respiratory viral infections through the development of host defence enhancers which locally prime and boost the body’s natural first line of defence against invading pathogens ENA’s approach offers a solution to protect against common and emerging respiratory viruses for which vaccines or direct-acting antivirals have limitations or do not exist those with an underlying medical condition (including chronic lung conditions and cardiovascular disease) and individuals with occupational risk (e.g INNA-051 is a potent agonist of toll-like receptor 2/6 (TLR2/6) which plays a key role in recognising pathogens and potentiating innate immune responses With a safety profile supporting seasonal prophylaxis use it has demonstrated accelerated virus clearance and stimulation of antiviral host defences in a Phase IIa proof-of-principle study using a human influenza-challenge model the company has raised US$33 million (AU$46 million) in financing from Brandon Capital It is partnered with the US COPD Foundation to support patient-centered clinical development of INNA-051 in COPD and has been awarded a US$13.1 million contract from the U.S a joint initiative between Johnson & Johnson Innovation and BARDA designed to accelerate novel potential solutions for future pandemics For more information, please visit https://enarespiratory.com Follow us on LinkedIn Kirrily Davis, E: kdavis@bcpvc.com M: +61 (0)401 220228 Sue Charles, E: sue.charles@charles-consultants.com M: +44 (0)7968 726585 Chris Gardner, E: Chris@CGComms.onmicrosoft.com M: +44 (0)7956 031077 2025 (ENA) -- The African Union’s Smart Safety Surveillance (AU-3S) program spearheaded by African Union Development Agency (AUDA-NEPAD) is advancing drug safety in Africa through innovation and regional collaboration.  AU-3S is supporting 12 African countries in building a continental database to track the safety of essential medicines and vaccines This data-driven network enables health authorities to report and respond to adverse drug events in real time—strengthening regulatory systems and enhancing public health outcomes from event reporting to signal management and ensure that no one is left behind.” He emphasized AU-3S’s collaboration with regional economic communities to promote regulatory harmonization and improve access to safe and effective medical products Nigeria’s pharmacovigilance system was underdeveloped “Joining the AU-3S platform helped us tremendously especially in reaching WHO maturity level three The digital tools and safety app significantly boosted the quality and volume of reports while harmonizing standards,” she said Secretary of Uganda’s National Drug Authority on his part stressed the importance of regional cooperation African countries must work together to regulate medicines and promote local production We need to reduce this reliance and manufacture within the continent,” Nahamya stated He called for leveraging Africa’s common market to scale pharmaceutical manufacturing investing in scientific validation of indigenous medicine and improving data sharing to strengthen drug safety oversight “Medicines are one of the most expensive parts of healthcare,” he said affordable medicines requires coordinated action and long-term investment in stronger health systems.” AU-3S now covers seven additional products in five major disease areas The program promotes safer and more accessible medical products while strengthening health system resilience across the continent the program is funded by the Bill and Melinda Gates Foundation Metrics details spores can form an infectious particle and can take up a central role in the environmental persistence and dissemination of disease A poorly understood aspect of spore-mediated infection is the fibrous structures or ‘endospore appendages’ (ENAs) that have been seen to decorate the spores of pathogenic Bacilli and Clostridia Current methodological approaches are opening a window on these long enigmatic structures Alphafold modelling and genetic approaches we identify a sub-class of robust ENAs in a Bacillus paranthracis foodborne outbreak strain We demonstrate that L-ENA are encoded by a rare three-gene cluster (ena3) that contains all components for the self-assembly of ladder-like protein nanofibers of stacked heptameric rings and their termination in a trimeric ‘ruffle’ made of a complement C1Q-like BclA paralogue The role of ENA fibers in spore-spore interaction and the distribution of L-ENA operon as mobile genetic elements in B strains suggest that L-ENA fibers may increase the survival These pili are primarily found in pathogenic species (i.e. by binding to host tissues and/or mediating self-contact in multicellular communities or biofilms Endospores represent an important dissemination form in pathogenic Bacillaceae ENAs may mediate cell-cell and cell-tissue interaction we use a combined cryoEM-genome mining approach to identify the major structural subunit of L-ENA (hereafter referred to as Ena3A) and solve the helical fiber ultrastructure to a resolution of 3.3 Å We show that ena3A is embedded in a three-gene cluster that holds all components for L-ENA assembly and anchoring to the exosporium a Composite montage of a single endospore with a central spore body and the surrounding exosporial sack b High-magnification image of the exosporium decorated with the hairy nap and two types of endospore appendages (L- and S-ENA) exhibit a ‘ladder-like’ pattern (4.6 nm intervals) are terminally decorated with single tip fibrillae Ruffles consist of a 45 nm long stalk that terminates into a globular head domain e reconstructed cryoEM-volume of L-ENA fibers (5.8 Å at FSC = 0.143) with rigid body docking of 7 copies of Ena1B19-117 f nsTEM image of recEna3A produced in and purified from the cytoplasm of E The data shown are representative of experiments made independently in triplicate a Helical ultrastructure of L-ENA determined at 3.32 Å global resolution (cut-off criterion at FSC = 0.143 Å−1) revealing an axial stacking of heptameric Ena3A rings that rotate 18.5° clockwise relative to each other Two neighboring Ena3A monomers are colored in blue and cyan b top-view of a single ring with a highlight of the β-sheet augmentation between the blue and cyan subunit two subunits were removed from the ring highlighted with an asterisk in (a) - showing the docking of the N-terminal connectors that covalently tether the ring docked above d Highlight of the lateral intra-ring contacts: two types of disulfide bridges (Cys14-Cys15; Cys22-Cys82) exist within two neighboring subunits each subunit connects to a subunit in the ring below via their Ntc (Cys8-Cys21; inset in stick representation) e On-axis top-view of the L-ENA fiber model in cartoon with disulfide bridges shown in red; f 2D class averages of straight and curved L-ENA segments covering 4 and 9 rings the 2D class average images of all treatments (apart from the 100% FA) are very similar to the 2D class averages obtained from ex vivo purified fibers We were not able to produce 2D class averages for the 100% FA-treated sample likely indicating that the Ena3A monomers had (partially) unfolded the FA-treated fibers did not depolymerize suggesting that disulfide bonds had not been reduced We conclude that the extensive hydrogen bonding between the Ena3A subunits combined with the covalent cross-links underlies the physico-chemical robustness of the L-ENA fibers Ena3A rings exhibit a rocking motion normal to the fiber axis with the respective hinge points centered on the Ntcs L-ENA flexibility can be traced back to (i) the spatial separation between consecutive rings thereby providing the possibility for local ring displacement without introducing steric clashes (ii) combined with the intrinsic flexibility of the N-terminal connectors a Side and b on-axis view of an S-ENA fiber (pdb-id: 7A02) composed of Ena1B subunits c Dimeric contacts of Ena1B subunits via β-sheet augmentation at the interface between the G and C strands Subunits are tilted 28° with respect to the fiber axis This out-of-plane interaction leads to a helical stacking of Ena1B monomers d Side and e on-axis view of an L-ENA fiber (pdb-id: 8PDZ) composed of Ena3A subunits f Dimeric contacts of Ena3A subunits via β-sheet augmentation at the interface between the G and C strands Although subunits are tilted 14° with respect to the fiber axis their contacts remain in-plane yielding a heptameric ring and primers used in standard PCR using cDNA template; b The L-ENA genes l-bclA and ena3A form an operon Agarose gel electrophoresis (1%) of PCR products conducted on cDNA from NVH 0075-95 culture (4 Primer combinations and expected product sizes are shown in (a) Genomic DNA (gDNA) from NVH 0075-95 and cDNA prepared from its isogenic Δena1ABC/Δena3A mutant were used as positive and negative controls for the PCR respectively; c Expression of L-ENA genes relative to rpoB during vegetative growth and sporulation Data are presented as mean values with error bars representing the standard deviation of three independent experiments Source data are provided as a Source Data file Thirteen genomes with an L-ENA gene cluster were complete and closed and the genomic location of the gene cluster could be inspected The ena3 gene cluster was located either on the chromosome (n = 7 genomes) on plasmids (n = 1 genome) or were found as paralogs on one or more plasmids and on the chromosome (n = 5 genomes) The ena3 gene cluster of NVH 0075-95 is flanked by an incomplete topoisomerase downstream and a complete Tn3 family transposase upstream (Fig. 6) This gene synteny was found in only two other strains in the cblaster/clinker analysis: B cereus AFS093282 (NZ_NVMQ01000017.1) and B pacificus strain BC444B (accession NZ_JAOPRQ010000006.1) the L-ENA gene cluster was flanked by frameshifted versions of Tn3 family transposases A few strains had a shorter Tn3 family transposase upstream of the L-ENA gene clusters the L-ENA gene cluster is/or has been located on a transposon and is part of the mobilome of the B accounting for the polyphyletic distribution of this gene cluster in the population nsTEM micrographs of the respective L-ENA operon deletion mutants a the ena3A gene is embedded in a three-gene cluster preceded on the CP116205.1 plasmid by two genes here annotated as exsL and l-bclA b Domain organization of the L-ENA proteins as identified by Interpro (10.1093/nar/gkac993) ExsL: consists of an N-terminal spore coat protein Z/Y domain and a C-terminal Ena-core domain (DUF3992) L-BclA: consists of an N-terminal collagen-like domain and a C-terminal BclA-C domain Ena3A: consists of a single Ena-core domain (DUF3992) AlphaFold2 predictions are shown in cartoon representation the base building unit of the exosporium lattice Inspection of the N-terminal region of L-BclA demonstrates the absence of an exosporium leader sequence which indicates that this collagen-like protein is likely not targeted to the exosporium protein ultrastructures composed of heptameric Ena3A rings that stack axially the L-ENA fiber terminates into a bacterial collagen-like ruffle protein The L-ENA fiber protrudes through the hairy nap layer while being tethered to the exosporium via a dedicated anchoring protein ExsL ExsL is expected to couple to or be integrated in the 2D crystal lattice of the exosporium the L-ENA fibers are depicted shorter here The strategic spatial positioning of pilus-presented adhesins promotes efficient attachment and there is a high probability that the ruffles of Ena’s exert related functions The AF2 model for the ExsL-ExsY dimer suggests that ExsL could be embedded within the ExsY lattice by mimicking the homotypic ExsY contacts including the formation of an inter-molecular disulfide bridge the AF2 model for an ExsL-Ena3A dimer is reminiscent of the Ena3A contacts found in the L-ENA cryoEM model – this in turn also includes one of the intra-ring Ena3A disulfide bridges we hypothesize that ExsL acts as a bridging moiety that anchors the L-ENA pilus onto the spore surface by maintaining an unbroken chain of disulfide cross-links across the various contact points (i.e. The experimental determination of the respective stoichiometries and the elucidation of the complex epitopes will be the subject of further study Negative stain TEM (nsTEM) imaging of bacterial spores and recEna3A filaments was done using formvar/carbon-coated copper grids (Electron Microscopy Sciences) with a 400-hole mesh The grids were glow discharged (ELMO; Agar Scientific) with 4 mA plasma current for 45 s 3 μl of a bacterial spore suspension or recEna3A protein solution was applied onto the glow-discharged grids and left to adsorb for 1 min followed by three washes with 15 μl Milli-Q 15 μl drops of 2% uranyl acetate were applied three times for 10 s with a blotting step in between each application The excess uranyl acetate was then dry blotted with Whatman type 1 paper All grids were screened with a 120 kV JEOL 1400 microscope equipped with LaB6 filament and TVIPS F416 CCD camera QUANTIFOIL® holey Cu 400 mesh grids with 2‐µm holes and 1‐µm spacing were glow discharged in vacuum using plasma current of 5 mA for 1 min (ELMO; Agar Scientific) 3 µl of 0.6 mg/ml graphene oxide (GO) solution was applied onto the grid and incubated 1 min for absorption at room temperature Excess GO was blotted using a Whatman grade 1 filter paper and left to dry under ambient conditions 3 µl of fiber suspension was applied on the GO‐coated grids at 100% humidity and room temperature in a Gatan CP3 cryo‐plunger the grid was machine‐blotted with Whatman grade 2 filter paper for 3.5 s from both sides and plunge frozen into liquid ethane at −176 °C Grids were stored in liquid nitrogen until data collection Two datasets were collected for ex vivo and recEna3A appendages with slight changes in the collection parameters High‐resolution cryoEM movies were recorded on a JEOL CRYO ARM 300 microscope equipped with omega energy filter and a K2 or K3 direct electron detector run in counting mode the microscope was equipped with a K2 summit detector and had the following settings: 300 keV The recEna3A dataset was recorded using a K3 detector and a total exposure of 64.66 e−/Å2 accrued over 60 frames/movie DNA fragment containing a 300 bp region upstream of the start codon of the l-bclA-ena3A operon and open reading frames of the respective genes was ordered (Synbio Technologies LLC) or amplified by PCR and cloned in the low copy number plasmid pHT304 at EcoRI restriction site we used nutrient broth (oxoid) in place of bacto medium (Difco) a sample of 20 ml culture was withdrawn every four hours (4 h and cell pellets frozen immediately at −80 °C until RNA extraction we collected 1 ml of the sample every hour and stained it with 10 µM of the membrane dye FM4-64 (MedChemExpress followed by a 2-min incubation at room temperature 3 µl of the stained sample was applied to a thin layer of 1.5% agarose (w/v) (Sigma Aldrich # A9539) solidified on a 10-well multitest slide (MP Biomedicals The slide was incubated for 1 min at room temperature in a biological safety cabinet to immobilize the sample and subsequently covered with a standard coverslip Images were captured using the 100× phase-contrast objective (Plan Apo λ Nikon) of the Nikon Eclipse Ti2 fluorescence microscope equipped with a DS-Qi2 camera Exposure times were set to 1 s and 200 ms for the 100× phase-contrast and TRITC RNA was extracted using Purelink RNA minikit (Ambion Life Technologies) according to the manufacturer’s protocol with a slight modification cell pellets were thawed on ice followed by addition of 200 µl of lysozyme solution (10 mM Tris-HCl (pH 8) 10 mg/ml lysozyme) and incubation for 5 min at RT A volume of 1 µl of 10% SDS was added to the cell suspension and vortexed after which 150 µl was transferred to new RNAse-free tubes Following addition of 350 µl of lysis buffer the cells were lysed by bead beating (FastPrep®-24 MP Biomedicals) for a total of two minutes at Speed 6 The lysates were centrifuged for 5 min (2600 × g) and the supernatants were transferred to new RNAse-free tubes The remaining steps were carried out as described in the manufacturer’s protocol RNA concentration was measured using Nanodrop 1000 (Thermo Scientific) and 1.5 µg RNA was treated with Turbo DNAase (Ambion) in 20 µl reaction volume following the manufacturer’s instruction and their efficiencies were calculated from the slope of the standard curve using the formula (10−1/slope-1) × 100 No template was added to the negative control samples The Ct values obtained for the 4-h samples were used as the calibrator sample in the data analysis and rpoB served as an internal control gene Each cDNA sample was analyzed in triplicate and the experiment was repeated independently The cycling conditions of the qRT-PCR were: 50 °C/2 min To determine if exsL, bclA, and ena3A are co-expressed, standard PCR was conducted using the cDNAs as templates. Primers that span across the indicated genes (Table S4) and DreamTaq PCR master mix (Thermo Scientific™) were used Purified genomic DNA (gDNA) from NVH 0075-95 and cDNA from NVH 0075-95 ΔenaABC/Δena3A (4 h and 16 h) were used as positive and negative controls the strain was subjected to hybrid assembly of long- and short-read sequences Genomic DNA from NVH 0075-95 was prepared from bacteria grown overnight on blood agar plates All sequencing was performed by Novogene Co UK) using a combination of Pacific Biosciences (PacBio) RS II single-molecule real-time (SMRT) sequencing platform with a with a SMRTbell template library and an Illumina NovaSeq 6000 platform (2 × 150 bp) with an insert size of 300 bp Data is available at NCBI under accession number GCA_027945115.1 Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article The Bacillus subtilis endospore: assembly and functions of the multilayered coat Bacterial spore germination receptors are nutrient-gated ion channels Cryo-EM analysis of the organization of BclA and BxpB in the Bacillus anthracis exosporium High resolution FESEM and TEM reveal bacterial spore attachment Surface architecture of endospores of the Bacillus cereus/anthracis/thuringiensis family at the subnanometer scale Bacillus anthracis spore surface protein BclA mediates complement factor H binding to spores and promotes spore persistence The spore antigens of Clostridium sporogenes Morphology of the spore of some strains of Clostridium botulinum type E The morphology and ultrastructure of the spore and exosporium of some Clostridium species Filamentous appendages of Bacillus cereus spores Spore appendages and taxonomy of Clostridium sordellii A.) 369–413 (Springer International Publishing Architects at the bacterial surface — sortases and the assembly of pili with isopeptide bonds an enzyme that anchors surface proteins to the cell wall Assembly of pili on the surface of Corynebacterium diphtheriae Properties and origin of filamentous appendages on spores of Bacillus cereus Endospore Appendages: a novel pilus superfamily from the endospores of pathogenic Bacilli Efficient Electrotransformation of Bacillus thuringiensis for Gene Manipulation and Expression Jonsmoen, U. L. et al. The role of endospore appendages in spore-spore contacts in pathogenic bacilli. Preprint at bioRxiv https://doi.org/10.1101/2024.04.22.590507 (2024) Challenges in homology search: HMMER3 and convergent evolution of coiled-coil regions UCSF ChimeraX: structure visualization for researchers UCSF ChimeraX: meeting modern challenges in visualization and analysis The anatomy and taxonomy of protein structure Global mRNA decay analysis at single nucleotide resolution reveals segmental and positional degradation patterns in a Gram-positive bacterium Highly accurate protein structure prediction with AlphaFold Richard, E. et al. Protein complex prediction with AlphaFold-Multimer. Preprint at bioRxiv https://doi.org/10.1101/2021.10.04.463034 (2022) ColabFold: making protein folding accessible to all Protein-protein interaction through beta-strand addition A collagen-like surface glycoprotein is a structural component of the Bacillus anthracis exosporium Polymorphism in the collagen-like region of the Bacillus anthracis BclA protein leads to variation in exosporium filament length The crystal structure of the Bacillus anthracis spore surface protein BclA shows remarkable similarity to mammalian proteins Targeting of the BclA and BclB proteins to the Bacillus anthracis spore surface Exosporia and appendages of spores of Bacillus species Protein composition of the outermost exosporium-like layer of Clostridium difficile 630 spores Characterisation of a large family of polymorphic collagen-like proteins in the endospore-forming bacterium Pasteuria ramosa Current insights on the diverse structures and functions in bacterial collagen-like proteins Glycosylation of BclA glycoprotein from Bacillus cereus and Bacillus anthracis exosporium is domain-specific The Bacillus anthracis Exosporium: what’s the big “hairy” deal Sequence motifs and proteolytic cleavage of the collagen-like glycoprotein BclA required for its attachment to the exosporium of Bacillus anthracis Crystal structure and induced stability of trimeric BxpB: implications for the assembly of BxpB-BclA complexes in the exosporium of Bacillus anthracis Inflammation-induced adhesin-receptor interaction provides a fitness advantage to uropathogenic E The biosynthesis and structures of bacterial pili Bacillus cereus sensu lato biofilm formation and its ecological importance Bacillus cereus spores and toxins - The potential role of biofilms Air-liquid interface biofilms of Bacillus cereus: formation Structural basis for Acinetobacter baumannii biofilm formation Molecular tiling on the surface of a bacterial spore - the exosporium of the Bacillus anthracis/cereus/thuringiensis group Routine markerless gene replacement in Bacillus anthracis influences multicellular behaviour and efficient production of virulence factors in Bacillus cereus A new mathematical model for relative quantification in real-time RT-PCR Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads Versatile genome assembly evaluation with QUAST-LG Bandage: interactive visualization of de novo genome assemblies BUSCO update: novel and streamlined workflows along with broader and deeper phylogenetic coverage for scoring of eukaryotic Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement TYGS and LPSN: a database tandem for fast and reliable genome-based classification and nomenclature of prokaryotes TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy PlasFlow: predicting plasmid sequences in metagenomic data using genome signatures NCBI prokaryotic genome annotation pipeline RefSeq: an update on prokaryotic genome annotation and curation RefSeq: expanding the Prokaryotic Genome Annotation Pipeline reach with protein family model curation cblaster: a remote search tool for rapid identification and visualization of homologous gene clusters Mashtree: a rapid comparison of whole genome sequence files Microreact: visualizing and sharing data for genomic epidemiology and phylogeography Download references We thank Dirk Reiter at the VIB-VUB Facility for Bio Electron Cryogenic Microscopy (BECM) and Yohannes Beyene Mekonnen at NMBU for technical assistance EOS Excellence in Research Program by FWO through grant G0G0818N to H.R recognizes the Grant from the Norwegian Research Council (NFR): 335029 - FORSKER22 Norwegian University of Life Sciences (NMBU) and cryo-EM imaging and data processing with assistance from M.F designed and generated the knockout strains and complementation constructs and prepared the spores for analysis with support from K.O.S conducted all sequencing and genomic analysis wrote the manuscript with contributions from N.V.G The authors declare no competing interests Nature Communications thanks Grace Taylor-Joyce and the other reviewers for their contribution to the peer review of this work Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41467-024-51804-w Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing newsletter — what matters in science Storyknife Did you know? Through its holding line Ena Holding and its subsidiaries Calista owns commercial real estate in Alaska We recently interviewed Ena Holding’s new president Karim Ders to learn more about the holding line’s business objectives and recent activities real estate markets that show strong growth and stability Our goal is to build long-term value while providing reliable financial returns and opportunities for Calista Shareholders we plan to sell properties that don’t align with our goals and reinvest in areas with better growth and income potential We are focusing more on warehouse properties because they offer steady returns and are less affected by economic downturns We’re also working with other Calista holding lines to find ways to collaborate we’re adopting new technologies and processes and procedures to streamline efficiency I have worked in real estate across the United States developing expertise in cross-border transactions I have worked for publicly traded Real Estate Investment Trusts (REITs) and private real estate investment companies serving clients such as pension funds and insurance companies My work has included managing property operations I have focused on strengthening Ena’s team understanding the Shareholders and communities we serve and conducting a comprehensive assessment of our properties Eek and Quinhagak has given me invaluable insights into Shareholders’ needs and the unique challenges in these communities we’ll continue embracing Calista’s core value of Continuous Improvement We’re dedicated to creating job opportunities for Calista Shareholders we’re building internship and apprenticeship programs to provide hands-on experience in the real estate field We’re also setting up training programs to help our current Shareholder employees grow in their careers We’re excited to support Calista’s mission and look forward to serving our Shareholders and communities in the years to come a California-based organization founded to advance innovative approaches for the prevention and treatment of influenza provided the funding to support ENA’s clinical development of INNA-051 seasonally delivered product designed to minimize the impact of viral respiratory infections It addresses the limitations of current vaccine and direct-acting antiviral approaches and could provide a critical advancement in reducing the continued impact of common and emerging respiratory viruses on vulnerable populations and the healthcare system INNA-051 is a host defence immune enhancer which locally primes and boosts the body’s innate immune response – the natural first line of defence The Flu Lab investment will support ENA’s planned Phase II proof-of-concept community infection study to assess the safety and potential efficacy of INNA-051 in reducing the incidence and duration of symptomatic infections caused by common respiratory viruses respiratory syncytial virus and human metapneumovirus in young adults at risk for exposure living in the US said: “There is an urgent need for new approaches to reduce the enormous burden of serious viral respiratory infections including influenza and this investment from Flu Lab will allow us to accelerate our clinical program We are delighted to have Flu Lab’s support and believe it is a strong endorsement of the work we have done to date in developing INNA-051 and its potential to make a meaningful difference in reducing mortality and morbidity.” ENA Respiratory is a clinical-stage pharmaceutical company tackling respiratory viral infections through the development of host defence immune enhancers which locally prime and boost the body’s innate immune response – the natural first line of defence against invading pathogens ENA’s antiviral host defence enhancers offer solutions to protect against common and emerging respiratory viruses for which vaccines or direct-acting antivirals have limitations or do not exist It is partnered with the US COPD Foundation to support the clinical development of INNA-051 in COPD and has been awarded a US$13.1 million contract from the U.S For more information, please visit https://enarespiratory.com Kirrily Davis, E: kdavis@bcpvc.com M: +61 (0)401 220228 Sue Charles, E: sue.charles@charles-consultants.com M: +44 (0)7968 726585 Chris Gardner, E: Chris@CGComms.onmicrosoft.com M: +44 (0)7956 031077 Scholarship and grant applications now available is looking forward to the opportunity to give back and mentor emergency nurses as the 2025 ENA Foundation chairperson emergency nursing is a calling and according to friends Russe has been on a path to leadership since she joined the Foundation board "I'm very honored to lead the ENA Foundation in 2025 and continue to make a positive impact in the lives of emergency nurses through the funding the Foundation provides I have been extremely blessed in my career as an ED nurse and I believe in paying it forward," said Russe "I wholeheartedly believe in the mission of the ENA Foundation and want to help make a difference especially in the area of education and research I'm looking forward to collaborating with our trustees to see what we can accomplish in 2025." which is a 501(c)(3) tax-exempt organization has strived to provide educational scholarships and research grants to those in the emergency nursing specialty the Foundation awarded more than $584,000 to 246 nurses through its various scholarships and grants 36 were awarded emergency relief grants which assist nurses going through hardship or natural disaster recovery The 2025 ENA Foundation Board of Trustees includes: ENA Media Contact:Morgan WietechaMedia Relations Strategist847.460.4038[email protected] Emergency nurses from across the country took to the nation's capital on Wednesday to emphasize the need for passage of legislation focused on.. Personnel Announcements 2025 (ENA)—Ethiopia is advancing its manufacturing sector through increased investment opportunities These efforts were prominently showcased at the third ‘Made in Ethiopia’ Expo held in Addis Ababa where government officials and industry leaders gathered to review progress and promote collaboration Zeleke Temesgen highlighted the country's ongoing initiatives to create a stable and attractive investment climate  He emphasized the government's dual approach: promoting Ethiopia's investment potential internationally while strengthening collaboration with domestic stakeholders to improve the business environment  A key discussion panel on the second day of the expo focused on "Expanding the Manufacturing Industry's Global Export Market Access Options and Integrating into Global Trade Systems."  Commissioner Zeleke stressed that Ethiopia is working to convert its vast potential into tangible outcomes by improving logistics and connecting international buyers with local producers the Ethiopian Investment Commission has launched a campaign targeting over 3,000 internationally recognized companies to invest in Ethiopia aiming to boost both foreign direct investment and local participation in manufacturing  State Minister of Trade and Regional Integration reiterated the government’s commitment to export growth  He said Ethiopia is prioritizing both continental and global markets and aggressively pursuing market access through bilateral The state minister also noted that Ethiopia has completed five rounds of WTO accession talks and is finalizing preparations for the African Continental Free Trade Area (CFTA) pilot project a landmark agreement among 52 African nations  He emphasized the sector’s potential to tap into the CFTA’s $3 trillion market and called for greater use of science and technology to improve quality Director General of the Ethiopian Capital Market Authority (ECMA)  Hana Tehelku emphasized role of capital markets in industrial transformation  She announced the rollout of long-term financing tools and highlighted the implementation of new legal frameworks aimed at increasing market competitiveness  Hana also urged manufacturers to embrace financial innovations and strengthen partnerships with foreign investors to ensure sustainable  The third Made in Ethiopia Expo ultimately served as a critical platform for aligning public and private sector efforts to advance Ethiopia’s integration into the global economy and strengthen its domestic manufacturing base Russia’s JSC ENA has delivered 16 pumps of the AKA-Zh 200-200-250-K-5-T4 type 2 safety classes quality assurance categories QA2 to units 3&4 of India’s Kudankulam NPP The supplied pumps are part of the refrigeration system for responsible consumers which is a critically important system for maintaining stable operation of a nuclear power plant JSC ENA has been supplying pumping equipment to Kudankulam NPP for more than 20 years This was the first time the company manufactured pumps of safety class 2 quality assurance category QA2 for Kudankulam which means that the entire design and production process was strictly controlled by an authorized organisation and representatives of the customer (IKAEL) at each stage Secondly these are the most high-performance pumps supplied to Kudankulam NPP Joint-stock company ENA was established in 1992 on the basis of the Shchelkovsky pump plant ENA is a leading Russian manufacturer of pumping equipment The company produces a range of centrifugal pumps for industry including for NPPs and the Navy the plant has specialised in the manufacture of pumps of II and III safety classes for NPPs Give your business an edge with our leading industry insights View all newsletters from across the Progressive Media network © Business Trade Media International Limited ENA's 54th president to emphasize advocacy 2025 /PRNewswire/ -- The passion for emergency nursing started early for the new Emergency Nurses Association President Ryan Oglesby From watching medical shows on TV to earning scout badges in first aid and then obtaining EMT certification Oglesby worked his way to a career in emergency nursing that has included ENA for two decades "It's an honor to be able to lead an organization that has given so much to me," said ENA President Ryan Oglesby "I'm really looking forward to connecting with members and showing them what is possible – that individually and as a group we can make a difference." In his seventh year on the ENA Board of Directors Oglesby looks to bring the voice of emergency nurses to the forefront during his presidential year Oglesby brings over 25 years of emergency care to this role working his way from an EMT to the assistant vice president for HCA Healthcare's South Florida Patient Logistics Center holding various committee positions and president of the North Carolina ENA State Council in 2012 He has also served as ENA secretary/treasurer and most recently "I hope to lead our board and members to continue the great work that has already been done to advance the emergency nursing specialty by providing quality education and resources and advocacy efforts around workplace violence and staffing," Oglesby said The full 2025 ENA Board of Directors also includes: ENA Media Contact:Morgan WietechaMedia Relations Strategist 847.460.4038 [email protected] It is with heavy hearts that we announce the passing of Mrs a native of Chauvin and resident of Montegut She was a cook at Boudreaux Canal and Lacache schools Bethany Chiasson (Tommy) and Casey Robichaux (Shana); great grandchildren Maddox and Walker Robichaux; great-great grandchild parents Alexander and Therese Blanchard; 6 siblings Betty Babin (Jesse) and Emma Battise (Richard) We would like to invite family and friends to attend a visitation in honor of Ena on Saturday Teaty for offering medical care and support Ena remained at her home and was surrounded by family and friends the family requests that donations be made to St Chauvin Funeral Home is honored to serve the family of Ena Savoie Essential digital access to quality FT journalism on any device Complete digital access to quality FT journalism with expert analysis from industry leaders Complete digital access to quality analysis and expert insights complemented with our award-winning Weekend Print edition Terms & Conditions apply Discover all the plans currently available in your country See why over a million readers pay to read the Financial Times MELBOURNE, Australia, April 02, 2025 (GLOBE NEWSWIRE) -- ENA Respiratory announces today the early view publication of results of a Phase I extension study in older adults (66-80) of its liquid formulation of INNA-051 a leading research journal of the European Respiratory Society1 In a placebo-controlled dose-escalation Phase 1 study a cohort of healthy older volunteers (66 – 80 years) received repeated intranasal doses of INNA-051 Biomarker analysis showed that INNA-051 triggered host defence pathways within eight hours of each dose The publication also includes supportive preclinical data Dosing of INNA-051 in aged mice challenged with H3N2 seasonal influenza virus was shown to stimulate natural nasopharyngeal host defences and resulted in reduced viral dissemination to the lungs Medicine Development Leader (Consultant) at ENA Respiratory said: “These newly published results form part of a growing body of data supporting the potential of INNA-051 as a new virus-agnostic approach to protecting people from significant morbidity and mortality resulting from respiratory viral infections Increased age is a major risk factor for such infections and these data demonstrate that INNA-051 is well-tolerated in an older population and stimulates natural host defence pathways in the nose aiming to provide rapid clearance of respiratory viruses which may prevent severe disease.” ENA will soon report the full results of a recently completed Phase 1b study of a more stable dry powder formulation in adults aged 18-80 years Headline results demonstrate that INNA-051 was well-tolerated in all age groups and led to local activation of host defence pathways This study provides further evidence for INNA-051 development in a broad age range ENA is planning to initiate a Q4 2025 Phase II community infection study in the US to assess the potential efficacy of INNA-051 to reduce the incidence severity and duration of symptomatic infections caused by common respiratory viruses 1. ERJ Open Research 2025 01044-2024; DOI: https://doi.org/10.1183/23120541.01044-2024 For more information, please visit https://enarespiratory.com Follow us on LinkedIn Kirrily Davis, E: kdavis@bcpvc.com M: +61 (0)401 220228 Sue Charles, E: sue.charles@charles-consultants.com M: +44 (0)7968 726585 Chris Gardner, E: Chris@CGComms.onmicrosoft.com M: +44 (0)7956 031077 Moments before last October’s BYU football game against Arizona, linebacker’s coach Justin Ena stood proudly in the coaches’ box high atop LaVell Edwards Stadium and watched his wife Shari deliver the play of the game As the Cougar Marching Band belted out the national anthem for all to hear Shari Ena performed the song in sign language for those who couldn’t She is an extrovert and I’m an introvert,” Justin Ena told the “Y’s Guys” podcast this week “It has been an amazing journey with her because she will put herself out there and then she pulls me along Justin makes a living interacting with young linebackers. He prides himself on being clear and concise in the way he teaches the likes of Jack Kelly, Harrisson Taggert, Isaiah Glasker and the others who are fighting for playing time The success of his message is reflected by how they perform Communicating in his marriage requires the same effort with an even greater attention to detail — starting on their first date “I had to mouth things really well because she reads lips and so everything was mouthed and I was talking like that,” Justin said “I’m not a loud person when I’m off the field it could be as loud as possible and all I had to do was mouth (the words) and she would pick up on everything Watching Shari conquer her challenge motivates Justin to tackle his “It’s cool because she is such a loving person and she has been doing it her whole life,” he said Ena was a defensive star for the Cougars between 1998-2001 and led the team in tackles his junior and senior seasons Ena began coaching in 2008 and two years after meeting and marrying Shari Among the requirements in the linebackers room this off season is to maintain weight and muscle mass Isaiah Glasker and company weigh-in every day Ena wants his guys to tip the scales around 242 pounds heading into fall camp 1-ranked defense last season with 70 tackles He arrived at BYU in 2022 weighing 197 and will be 45 pounds heavier when Portland State comes to Provo on Aug They want to be the best at what they do,” Ena said BYU’s Big Three get most of the headlines and deservedly so Taggert and Kelly finished among the top five in total tackles and generated 27 tackles for loss But Ena is confident in the group right behind them “I think Ace may have had the best spring of all the linebackers,” Ena said 240-pound cousin of Maika and former Cougars Bronson The sophomore appeared in six games and had one tackle during last year’s 11-2 season “He’s one of those kids who you think is almost too good to be true because he’s a great human being,” Ena said “(When) I say something out of line sometimes I feel like there is a general authority across the way and he’s a 21-year-old kid Dave McCann is a sportswriter and columnist for the Deseret News and is a play-by-play announcer and show host for BYUtv/ESPN+. He co-hosts “Y’s Guys” at ysguys.com and is the author of the children’s book “C is for Cougar,” available at deseretbook.com Based on the surreal animated series from YouTuber Joel G ENA Dream BBQ is a new indie that's already rolling in positive Steam reviews Follow us for daily PC games news, guides and reviews on X, Facebook, Google News, and Steam. Or sign up to our free newsletter MEXC Backs Decentralized Stable Assets with USDe Listing Since their inception, stablecoins have played an important role in the crypto ecosystem. However, many face limitations due to dependence on centralized custodians and traditional banking infrastructure. USDe, issued by the Ethereum-based DeFi platform Ethena (ENA) It is a fully decentralized synthetic USD asset that uses delta-neutral hedging to maintain a soft peg to the U.S dollar without the need for overcollateralization or central custody USDe employs smart contracts to automatically open and close perpetual short positions As a global leader in digital asset trading MEXC's listing of USDe and USDE-related trading pairs highlights the growing importance of decentralized stable assets in the evolving DeFi landscape This initiative reaffirms MEXC's commitment to supporting innovative blockchain solutions and promoting decentralized finance By providing strong liquidity and broad market coverage MEXC creates the ideal environment for projects like USDe to thrive and unlock new possibilities in the digital economy MEXC also offers users the chance to participate in a $1,000,000 reward pool through four major activities This initiative enables users to engage with cutting-edge DeFi 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returns providing a multifaceted approach to digital asset growth that caters to both new and experienced market participants MEXC aims to become the go-to platform offering the widest range of valuable crypto assets The platform has grown its user base to 30 million by providing a diverse selection of tokens including 1,716 initial listings and 605 memecoins with total airdrop rewards exceeding $136 million MEXC is committed to being "Your Easiest Way to Crypto" Serving over 32 million users across 170+ countries MEXC is known for its broad selection of trending tokens Our user-friendly platform is designed to support both new traders and experienced investors offering secure and efficient access to digital assets MEXC prioritizes simplicity and innovation making crypto trading more accessible and rewarding Contact:Lucia HuPR Managerlucia.hu@mexc.com Disclaimer: This content is provided by MEXC and opinions expressed in this content are solely those of the content provider and do not necessarily reflect the views of this media platform or its publisher or reliability of any information presented This content is for informational purposes only and should not be considered financial Investing in crypto and mining related opportunities involves significant risks Readers are strongly encouraged to conduct their own research and consult with a qualified financial advisor before making any investment decisions due to the inherently speculative nature of the blockchain sector--including cryptocurrency and mining--complete accuracy cannot always be guaranteed Neither the media platform nor the publisher shall be held responsible for any fraudulent activities or financial losses arising from the content of this press release GreeceChevron SantoriniChevron OiaChevron For the chance to hide out in the chicest of Cyclades cave homes with unparalleled views over Santorini’s glittering caldera and excellent local cuisine a stone’s throw from Oia’s smart high street Sweep past baskets of grapes punctuating the roadside vineyards up to the fishing village of Oia you’ll reach a pearly grey pathway inviting you into Ena carved directly into the half moon of Santorini's glistening cliff face the Canaves Collection’s first hotel has just unveiled a total renovation Its 18 ultra-chic cave rooms are now all Santorinian marble and woods glassy infinity pools and bubbling jacuzzis with blistering Cycladic stucco walls set against flashes of pink bougainvillaea and watching the sun dip low over the yachts in the water grazing on spiced moussaka and filo honey-drizzled pies There may now be five hotels in the Canaves Collection but Ena remains the one to beat for a break for two The Chaidemenos family caves dutifully functioned as wine cellars from the 17th century right up until 1985 when Ioannis Chaidemenos and his wife Anna had other ideas Turning the cellars into guest rooms meant the barrels were out The aim was to create a hotel deserving of its spot on Santorini’s unique cliff sides but a few partial redesigns in the following 40 years gave Ena a disjointed feel Greek architect Daphne Zografou stepped up and a singular warm grey Cycladic path leads you around 18 blistering white cave suites a pool with swim-up bar and an excellent Greek restaurant overlooking sparkling caldera views The natural architecture has been preserved maintaining the imperfections of the lines and the structural integrity as it always has been It means even the least expensive rooms have some of the loveliest features – yes but follow the staircase down to the former wine cellar and you’ll find a cavernous The River Pool Suite has a pool that curves its way from the verandah right up to the bathroom window (guests that aren’t such sticklers for the rules have been known to enter this way The idea is to create the sense you’re hanging out chummily at grandma’s house with the added boon of glittery sea views from every seat in the house It’s mostly sharing plates; pillowy tomato fritters A mezze ring of terracotta pots arrives filled with the likes of anchovy-rich taramasalata fleshy olives and a side of generously-salted pita Moussaka is fluffy and sweetly spiced and a wodge of walnut pie glints with raspberries and a swirl of chocolate cream The hotel will arrange transfers to the collection’s other restaurants which we’d recommend if only for the octopus bolognese at Epitome’s Omnia (we asked but were forbidden knowledge of the spice blend used – house secret) also concealed within a 17th century former wine cave now containing two treatment rooms sauna and couples’ treatment room with caldera views with liberal drizzles of sweet fig and hazelnut oil and backdrop of twinkly candles will firmly tease out any remaining woes retained in your shoulders before you’re sent floating back to Ena Oia is the island’s second largest village after Fira and certainly the prettiest famous blue-domed churches and eclectic wibbly-wobbly cliffsides When not floating on a yacht at sunset (boat tours can be booked easily via the hotel’s WhatsApp concierge you’ll find a sophisticated bunch of tourists perusing the smart boutiques on the marble-tiled high street or sipping cocktails in the smattering of excellent bars and restaurants “Character is more important than skill,” says manager John-Nicholas Bubaris “There is lots of pressure and stress in the world so we want to provide comfort and simplicity to those who stay here that is luxury in 2024 – not bowing in front of guests.” with the ethos being that the staff complement the walls of the hotel; it’s the people that make up the true character of any space It’s paid off – all hires for 2023 returned for the 2024 season Even our taxi driver complimented John on his management is a better bet to book for those travelling with family along with eco-housekeeping and building methods Only locals are employed and nothing but seasonal Santorinian produce is served in the collection’s bars and restaurants But the Chaidemenos family has a longstanding commitment to sustainable tourism father to current managing director Markos He passed a law where no additional bars or restaurants could open without replacing an existing one to help the village’s small businesses collaborating with the Mayor of Santorini on a number of long-term over-tourism and environmental initiatives including the introduction of a tourist levy reducing the number of cruise passengers and limiting construction on Santorini for starters All listings featured on Condé Nast Traveler are independently selected by our editors. If you book something through our links, we may earn an affiliate commission. up-to-the-minute voice in all things travel Condé Nast Traveler is the global citizen’s bible and muse We understand that time is the greatest luxury which is why Condé Nast Traveler mines its network of experts and influencers so that you never waste a meal or a hotel stay wherever you are in the world 2025 (ENA) -- The First Global Public Security Cooperation Forum was held in Addis Ababa today Present at the form included the Ethiopian Federal Police Commissioner General Demelash Gebremichael Chairman of the Global Public Security Cooperation Forum members of the Eastern Africa Police Chiefs Cooperation (EAPCCO) and representatives of police commissions from various African countries Commissioner General Demelash Gebremichael indicated that the forum gives priority to collective security adding that Ethiopia is making significant contribution to foster peace at the continental level By participating at the Global Public Security Forum Ethiopia raised key issues of public safety indicated that the forum is making global efforts to address conflicts and their root causes Stating that the forum upholds collective security he stated that efforts are underway to encourage collective efforts The forum strives to ensure lasting peace and development at the global level adding that implementing innovative ideas is another dimension thereby upholding public well-being and fostering collaboration for economic development Participants of the forum on their part said that the Global Public Security Cooperation Forum offers a significant opportunity to work collaboratively in preventing terrorism They emphasized the importance of working in cooperation to combat cross-border crimes Recall that Ethiopia has been elected as Board of Director of the Global Public Security Cooperation Forum (Lianyungang) 2025 (ENA) --- Present-day Ethiopia is need of second generation patriots that will complement our liberty fully Ethiopians are commemorating the 84th anniversary of Patriots' Victory Day today Prime Minister Abiy extended a message to the Ethiopian people in connection with the 84th anniversary of  victory day In a message he shared on his social media page Prime Minister Abiy stated that our forefathers and foremothers handed down to us a nation with sacrifice paid dearly to maintain their liberty; and we are proud of it present-day Ethiopia is need of second generation patriots that complement our liberty fully he stressed and added these patriots will elevate the country to the overall prosperity and achieve the country's complete freedom He further said that such patriots are engaged in peaceful struggle on political social and diplomacy fronts and they defeat poverty and backwardness The tools of today’s patriotism are knowledge while the underlying passions are love for the country,  steadfastness and integrity Stating that realizing Ethiopia’s vision—to become prosperous on all fronts the prime minister emphasized the country needs both patriotism and this generation in particular is in need of the latter patriotism the Patriots’ Day reminds us to commemorate heroism of our ancestors I hope it also serve to secure the foundation for the second patriotism MELBOURNE, Australia, Dec. 18, 2024 (GLOBE NEWSWIRE) -- ENA Respiratory a clinical-stage pharmaceutical company developing innate immune modulators for the prevention of complications associated with respiratory viral infections in at-risk populations announces today the publication of results of its Phase IIa proof-of-principle study with its liquid formulation of INNA-051 in ERJ Open Research The publication also includes data from ENA Respiratory’s dose-escalation study to assess tolerability and mechanistic biomarkers following single and repeated intranasal administrations of INNA-051 in individuals aged 18-55 A virus-agnostic intranasal host defence immunomodulator is a potent first-in-class agonist of toll-like receptor 2/6 (TLR2/6) which plays a key role in recognizing pathogens and triggering the innate immune response The data presented in the publication demonstrate: said: “The publication of the results of these studies demonstrates the strength of the data supporting our continued development of INNA-051 as a potentially significant new approach to protecting people at risk of real harm from serious respiratory viral infections INNA-051 is designed to boost the body’s innate immune response – the natural first line of defence – directly at the site of infection and prevent complications relating to infection by common respiratory viruses or emerging new viruses The newly published data confirm pre-clinical data demonstrating that INNA-051 is well-tolerated and primes virus-agnostic local innate immune responses that boost host defence pathways.” The results of the publication announced today supported continued development of INNA-051 and the planned Phase II study to assess the potential of INNA-051 to prevent symptomatic viral respiratory infections in a community setting ENA is completing a Phase 1b study of a dry powder formulation of INNA-051 with an anticipated shelf life of over two years at room temperature Headline data demonstrated an acceptable safety profile and evidence of local activation of innate immune pathways in older adults 1. ERJ Open Res 2024 Dec 9;10(6):00199-2024. doi: 10.1183/23120541.00199-2024 ENA Respiratory is a clinical-stage pharmaceutical company tackling serious respiratory viral infections through the development of host defence immune modulators which locally prime and boost the body’s innate immune response – the natural first line of defence immune enhancers are complementary to often virus-specific vaccines and existing direct-acting antivirals once-a-week nasal dry powder product to prevent complications associated with respiratory viral infections in at-risk populations the company has raised US$26M (AU$44million) in equity financing from Brandon Capital For more information, please visit https://enarespiratory.com Follow us on LinkedIn: https://www.linkedin.com/company/enarespiratory-pty-ltd/ E: sue.charles@charles-consultants.com M: +44 (0)7968 726585 E: Chris@CGComms.onmicrosoft.com M: +44 (0)7956 031077 2025 (ENA) --- The African Union Commission has officially launched the Comprehensive African Agricultural Programme (CAADP) Strategy and Action Plan 2026-2035 and as the Kampala declaration at a ceremony held in South Africa This declaration is crucial as it sets the stage for the ambitious CAADP Action Plan 2026-2035 Blue economy and sustainable Environment (ARBE) during the launch said a seed of hope has been planted in the fertile soil of Africa’s agricultural landscape Vilakati said they gather to nurture this seed into a bountiful harvest symbolizing the transformation of the agriculture sector and the prosperity of the African people “Our Journey so far in Agriculture has made us traverse the path from the Maputo Declaration to the Malabo Declaration we embark on a new journey with the Kampala Declaration and the CAADP Strategy and Action Plan 2025-2036 Despite various challenges faced including budgetary constraints African Union Member States remain resilient to the commitment of CAADP.” many countries have made commendable progress though the targets set by the Malabo Commitments have not been fully achieved extending gratitude to  the African Union Member States for the fortitude shown to make Africa self-reliant in terms of food Self Sufficiency The new CAADP Strategy and Action Plan 2025-2036 marks a significant shift towards agrifood systems transformation “We recognize that agriculture is not just about production but about creating a holistic system that encompasses production It also envisions sustainable and resilient agri-food systems for a healthy and prosperous Africa.” the continent has been offered a springboard to create greater coherence in the bid to drive nature-positive agricultural production systems that will support ecosystem restoration and bio-diversity gain Africa is home to nine of the 10 countries most vulnerable to climate change and African Union Member states are already diverting up to 9 per cent of their national budgets to respond to climate extremes Moses Vilakati finally urged member states to support the domestication of the CAADP Strategy and Action Plan (2026-2035) through committing increased budgetary allocations 2025 (ENA) -- Ethiopian Minister of Foreign Affairs Gedion Timothewos and Israeli Foreign Minister Gideon Sa'ar held high-level talks in Addis Ababa signaling a strong mutual commitment to reinforcing the historic and multifaceted diplomatic relations between their nations which followed Minister Gedion Timothewos's earlier visit to Israel underscored the deep-rooted friendship and strategic importance both countries place on their partnership both ministers focused on strengthening bilateral cooperation across various sectors and addressing shared regional and international concerns reflecting on the significance of his visit reflects our will to strengthen the cooperation and the relations between our countries." He emphasized the profound historical connection "The relationship between Ethiopia and Israel is deep and historic Minister Sa'ar also highlighted Ethiopia's crucial role as a regional partner explaining his choice for his first visit to Africa as foreign minister: which demonstrates the importance of your country to us and we want to upgrade the relationship between the countries in all aspects." He further described Ethiopia as "a gateway to Africa for us," acknowledging the vital link provided by Ethiopian Airlines which continued flights to Israel "almost without interruption during the war." Ethiopian Foreign Minister Gedion Timothewos reciprocated the sentiment expressing his pleasure in hosting his counterpart it's a great privilege and honor to host you here in Addis I'm very glad that you have honored your promise to come and visit very soon after my visit to Jerusalem." He stressed the economic dimension of the relationship and economic cooperation between the two countries would serve as an anchor to a comprehensive strategic partnership between Israel and Ethiopia." The ministers discussed various sectors of mutual interest Minister Gedion Timothewos specifically highlighted the success of existing cooperation very important in transforming horticulture practices in Ethiopia our avocado exports have boomed thanks to Israeli technical cooperation." Both ministers expressed their commitment to expanding collaboration in these vital areas Minister Sa'ar is accompanied by a business delegation with a large Israel business forum scheduled for tomorrow which he noted "includes dozens of public and private sector participants from both countries seeking new business and collaboration opportunities." "We are committed to expanding our cooperation in all areas We are committed to realizing the great potential in trade and economic partnerships." A significant portion of the discussion was dedicated to the shared threat of terrorism "Terrorism is a common threat to our nations." He said they must be defeated." Minister Gedion Timothewos concurred on the severity of the threat "Terrorism is a trait both to Israel and Ethiopia He emphasized the importance of continued collaboration in this area: ".. our continued and strengthened cooperation in this sector is very important to counter terrorism in the Horn of Africa and in the Middle East." The ministers concluded their meeting by reaffirming their commitment to strengthening the longstanding bilateral relations Minister Gedion Timothewos expressed hope that the visit "will provide further impetus and momentum to this growing and very critical partnership for both countries." Minister Sa'ar's visit to Addis Ababa and the high-level discussions underscore a renewed focus on deepening diplomatic and security ties between Ethiopia and Israel at a crucial time for both nations and the wider region 2025 (ENA) ---President Taye Atseke Slassie emphasized the importance of emulating the patriotism and dedication of Ethiopian heroic patriots.  The president made these remarks as the nation is commemorating the 84th Patriots' Victory Day at the Patriots’ Memorial Victory Monument in Addis Ababa President Taye laid a wreath in honor of those who fought for Ethiopia’s freedom Among those in attendance were Lij Daniel Jote Mesfin President of the Ethiopian Patriots Association leaders and members of the National Defense Force president Taye remarked that the victory of the Ethiopian patriots was a historic stand against fascism and colonialism serving as an inspiration of resistance for the world He underscored the need for present and future generations to emulate the bravery and selflessness of the patriots by contributing to the nation’s ongoing development it is impossible to stop or hinder Ethiopia's progress,” he noted Director General of Psychological Development within the National Defense Forces noted that Patriots’ Victory Day is a solemn occasion to honor those who sacrificed their lives for Ethiopia's freedom and dignity He also called for greater awareness among the younger generation about the high price paid for national sovereignty emphasized the city administration's commitment to preserving and enhancing heritage sites Renovation was made to the Patriots’ Victory Monument and its surroundings aiming to ensure the area remain a proud symbol for future generations Lij Daniel Jote Mesfin on his part praised the beautification efforts around the Arat Kilo area saying the revitalized infrastructure has elevated the holiday’s significance.  He also called on the youth to carry forward the legacy of the patriots by contributing to peace and national development