This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page Kisumi Omori and Kokoro Fujishiro couldn't find the slightest opportunity to score during normal time Kisumi Omori scored a waza-ari to take the gold medal Khorloodoi Bishrelt (UAE) found herself facing Rin Takeuchi (JPN) during the first contest for a bronze medal Bishrelt scored a first waza-ari but Takeuchi came back with an equal score Khorloodoi Bishrelt eventually launched a yoko-guruma that sent her opponent flat on her back for ippon and a bronze medal Sosorbaram Lkhagvasuren (MGL) and Nanako Tsubone (JPN) could still be in the ranking of the category by facing each other for a bronze medal Golden score was needed to determine the winner nothing seemed to offer an idea of who the winner would be until Nanako Tsubone engaged with a low circling o-uchi-gari for waza-ari 2025 / Interviewing an Olympic champion is something special .. 2025 / The draw for the 2025 Baku Grand Slam is imminent and .. 2025 / There is a large Japanese team in Paris but several .. 2024 / As a symbol of the impressive results of the Japanese .. 2024 / Two-time junior world champion (Guayaquil and Odivelas) 2024 / The initial question of the day was clearly answered .. 2024 / With only one known international result Anticipation is building toward the release of One-Punch Man Season 3, especially now that it has been confirmed that the anime series will return this October fans still haven't seen much of the upcoming third season Aside from a Special Announcement trailer released last year and a batch of character illustrations released through the Hero Visual Project Staff — the studio animating the third season — really hasn't shared many details The lack of details has some fans concerned that One-Punch Man Season 3 won't live up to the hype Or that it could follow in the steps of Season 2 which saw a noticeable drop in quality compared to the first season Staff producer Atsushi Fujishiro has offered a hopeful promise "Preparations for the third season are steadily progressing," Fujishiro said prior to this week's release window announcement "As a highly popular title with high expectations we’re committed to delivering a season that won’t disappoint.” many fans acknowledged that there was a major drop in quality from Season 1 to Season 2 In addition to the poorer animation quality and just about everything else you can think of It should be noted that although production shifted from Madhouse to J.C It's widely acknowledged that the studio faced an incredibly rushed production schedule which resulted in underwhelming production value That hopefully won't be the case with Season 3 which was first announced back in 2022 One would thing with three years worth of production time Staff had all the time they needed to ensure that Season 3 is a return to form this week's release window announcement was only accompanied by a short promo video that assembled many of the heroes spotlighted in the Hero Visual Project It wasn't really a full-fledged trailer It's reported that the One-Punch Man Season 3 trailer will be shown at AnimeJapan 2025 later this month The annual anime convention takes place from March 22 to March 23 which is when we could get our first proper look at Season 3 all we've really had was the Special Announcement video and the character illustrations Saitama is a hero who only became a hero for fun After three years of "special training," hes become so strong that he's practically invincible he's too strong—even his mightiest opponents are taken out with a single punch Saitama performs his official hero duties as a member of the Hero Association monsters claiming to be from the Monster Association suddenly appeared taking a child of Hero Association executive as a hostage The S-class heroes gather and plan a raid on the Monster Association hideout to rescue the hostage a "human monster" who was taken by the Monster Association during a battle with the heroes awakens in the Monster Association hideout While there is understandable worry surrounding One-Punch Man Season 3 our fears will hopefully be put to rest once we see a proper trailer Please activate JavaScript function on your browser Metrics details Manipulating topological spin textures is a key for exploring unprecedented emergent electromagnetic phenomena Whereas switching control of magnetic skyrmions the transitions between a skyrmion-lattice phase and conventional magnetic orders is intensively studied towards development of future memory device concepts transitions among spin textures with different topological orders remain largely unexplored Here we develop a series of chiral magnets MnSi1−xGex serving as a platform for transitions among skyrmion- and hedgehog-lattice states Lorentz transmission electron microscopy and high-field transport measurements we observe three different topological spin textures with variation of the lattice constant controlled by Si/Ge substitution: two-dimensional skyrmion lattice in x = 0–0.25 and two distinct three-dimensional hedgehog lattices in x = 0.3–0.6 and x = 0.7–1 The emergence of various topological spin states in the chemical-pressure-controlled materials suggests a new route for direct manipulation of the spin-texture topology by facile mechanical methods The concept of topology provides a powerful scheme for the classification of electronic and magnetic states, and also for the description of their physical properties1 Topology of a magnetic structure is characterized by the winding number \(w = \frac{1}{{8{\mathrm{\pi }}}}\epsilon ^{ijk}{\kern 1pt} {\int}_S {{\kern 1pt} dS_k{\mathbf{n}}({\mathbf{r}}) \cdot [\partial _i{\mathbf{n}}({\mathbf{r}}) \times \partial _j{\mathbf{n}}({\mathbf{r}})]}\) This quantity counts how many times the direction of the local magnetization wraps the unit sphere within the unit area S When a magnetic structure possesses a non-zero integer winding number it behaves as a topologically stable spin-object producing emergent phenomena unique to its topological class f Composition x dependence of ferromagnetic transition field Hc (e) and saturation magnetization Ms (f) In order to harness the topological properties unique to each spin texture and to explore non-trivial emergent phenomena at their transitions direct control of topology of spin texture is essential switching of the spin textures among plural different topologically nontrivial classes has remained a challenge We focus on chemical/mechanical pressure as one potential approach to this end by achieving dramatic modification in magnetic interactions through changing inter-atomic distances Our neutron scattering experiment and Lorentz transmission electron microscopy (LTEM) observation confirm the conventional SkL for Si-rich composition range (x = 0–0.25) tetrahedral-4q HL for the intermediate range (x = 0.3–0.6) and cubic-3q HL for Ge-rich range (x = 0.7–1) by high-field Hall resistivity measurements we identified topological Hall effect of Berry-phase origin in each magnetic phase supporting their topological spin arrangements The error bars for x ≤ 0.25 are defined by the spatial resolution of Lorentz transmission electron microscope while those for x ≥ 0.4 represent statistical error of one standard deviation of the SANS measurements \pm \frac{q}{{\sqrt 3 }}} \right)\) and \(\left( {\frac{{2{\mathrm{\pi }}}}{a} - q,\frac{{2{\mathrm{\pi }}}}{a},0} \right)\) the q-vector directions are along 〈111〉 crystal axes for x = 0.6 As summarized in Fig. 2h, there are two features in x-dependence of the magnetic modulation q; the two-step magnetic transitions observed as distinct changes in the phase diagram (Fig. 1d) One is the discontinuous variation in λ (=2π/q) at x ~ 0.3 and the other is change in the pinned q-direction at x ~ 0.7 It is therefore clear that three distinct magnetic phases are realized in MnSi1−xGex those respectively turn out to be helicl/SkL (x = 0–0.25) Further theoretical studies are desired to clarify the crucial magnetic interaction which takes over from DMI in the course of enlarging the lattice constant Magnetic structures of MnSi1−xGex revealed by small-angle neutron scattering (SANS) and Lorentz transmission electron microscopy (LTEM) under magnetic field a The SANS setup with magnetic field H perpendicular to the incident neutron beam b Schematic illustration of LTEM observation with H parallel to the incident electron beam (orange lines) where the blue arrows represent the in-plane magnetic moment configuration of a skyrmion μ0H = 0.3 T) in (001) crystal plane and its Fourier transform pattern d–f The SANS measurement points (gray dots) and sequences (blue arrows) are shown in the magnetic phase diagrams of x = 0.2 (d) where the blue stars represent the data points shown in g–i The SANS intensity patterns of x = 0.2 (T = 25 K The small white circles emphasize the characteristic peak intensities for each composition The candidate multiple-q structures explaining the observed SANS intensity patterns are shown in h–l where the yellow rings represent the rotation degrees of freedom of q-vectors due to randomly oriented crystal domains in the polycrystalline samples and the yellow dots represent the scattering intensities on the detector plane Here we note that we cannot exclude a possibility of multi-domain state of the single-q helical structure on the basis of the SANS results alone such a scenario is incompatible with the observed large topological Hall effect which is the hallmark of the formation of non-coplanar spin textures endowed with scalar spin chirality which may imperil the validity of the present estimation of topological Hall effect the magnitude and the sign changes of topological Hall resistivity may be difficult to quantitatively elucidate at the moment while the presence of non-coplanar spin texture manifests itself by such anomalously large signals of topological Hall resistivity the impact of pressure on topological spin textures deserves further investigations in a wide range of materials in the light of the exploration of novel spin textures and emergent electrodynamics Polycrystalline samples of MnSi1−xGex were prepared by the high-pressure synthesis technique and Ge were first mixed with stoichiometric ratio and then melted in an arc furnace under an argon atmosphere it was heated at 1073 K for 1 h under 5.5–6.0 GPa with a cubic-anvil-type high-pressure apparatus Powder x-ray analyses confirmed B20-type crystal structure (P213) Magnetization was measured either by using ACMS option with Physical Property Measurement System (PPMS) or by DC option with Magnetic Property Measurement System (MPMS) Magneto-resistivity and Hall-resistivity up to 14 T were measured by using AC-transport option with PPMS Magnetic field was applied perpendicular to electrical current Higher-field measurements of magnetization and Hall resistivity were performed utilizing non-destructive pulse magnets energized by capacitor banks and a flywheel DC generator installed at International MegaGauss Science Laboratory of Institute for Solid State Physics (ISSP) The high-field magnetization was measured up to 56 T by the conventional induction method The high-field resistivity was measured up to 30T using the long (~1s) field pulse with the AC four-wires method employing a numerical phase detection technique with a sampling rate of 200,000 data points per second and an excitation current of 10 kHz and 200 mAp-p Lorentz TEM observations for a (001) MnSi0.8Ge0.2 thin plate were performed using a multifunctional transmission electron microscope (JEM2800 JEOL) equipped with double-tilt helium cooling holder (Gatan ULTDT) The thin plate was prepared by an Ar+ milling process after mechanical polishing of the bulk sample A powder sample of MnSi1−xGex was packed in an aluminum container filled by He gas The weight of the powder sample was \(0.450\,{\mathrm{g}}\left( {x = 0.2} \right)\) \(0.719\,{\mathrm{g}}\left( {x = 0.4} \right)\) \(1.059\,{\mathrm{g}}\left( {x = 0.6} \right)\) and \(0.751\,{\mathrm{g}}\left( {x = 0.8} \right)\) Magnetic field was applied perpendicular to the incident neutron beam The diffracted neutron beam with the wavelength of \(0.5 < \lambda < 7.8\) Å was collected by four detector banks of small- and high-angle and backward detector banks and analysed by using time-of-flight (TOF) method The data sets generated during and/or analysed during the current study are available from the corresponding author on reasonable request The topological theory of defects in ordered media Thermodynamically stable “vortices” in magnetically ordered crystals: the mixed state of magnets Real space observation of a two-dimensional skyrmion crystal Noncentrosymmetric magnets hosting magnetic skyrmions Nanoscale magnetic skyrmions in metallic films and multilayers: a new twist for spintronics Magnetic skyrmions: advances in physics and potential applications Writing and deleting single magnetic skyrmions Current-induced skyrmion dynamics in constricted geometries stability and current-induced motion of isolated magnetic skyrmions in nanostructures Unusual hall effect anomaly in MnSi under pressure Topological hall effect in the a phase of MnSi Large topological hall effect in a short-period helimagnet MnGe Dynamics of skyrmion crystals in metallic thin films Current-induced rotational torques in the skymion lattice phase of chiral magnets Emergent electrodynamics of skyrmions in a chiral magnet Unwinding of a skyrmion lattice by magnetic monopoles Dynamics and energetics of emergent magnetic monopoles in chiral magnets Partial order in the non-Fermi-liquid phase of MnSi Formation of a topological non-Fermi liquid in MnSi Electrical magnetochiral effect induced by chiral spin fluctuations Possible skyrmion-lattice ground state in B20 chiral-lattice magnet MnGe as seen via small-angle neutron scattering Critical phenomena of emergent magnetic monopoles in a chiral magnet Large magneto-thermopower in MnGe with topological spin texture Two-step pressure-induced collapse of magnetic order in the MnGe chiral magnet Variation of skyrmion forms and their stability in MnSi thin plates Generic aspects of skyrmion lattices in chiral magnets First-principles evaluation of the Dzyaloshinskii–Moriya interaction Hidden quantum phase transition in Mn1−xFexGe evidenced by small-angle neutron scattering Effective bilinear-biquadratic model for noncoplanar ordering in itinerant magnets Multiple-q states and the skyrmion lattice of the triangular-lattice Heisenberg antiferromagnet under magnetic field Multiply periodic states and isolated skyrmions in an anisotropic frustrated magnet Topological properties and dynamics of magnetic skyrmions Spin chirality induced skew scattering and anomalous Hall effect in chiral magnets Versatile helimagnetic phases under magnetic fields in cubic perovskite SrFeO3 Ishiwata, S. et al. Emergent topological spin structures in a centrosymmetric cubic perovskite. Preprint at https://arxiv.org/abs/1806.02309 (2018) The design and q resolution of the small and wide angle neutron scattering instrument (TAIKAN) in J-PARC From ultrasoft pseudopotentials to the projector augmented-wave method Fully unconstrained noncollinear magnetism within the projector augmented-wave method Generalized gradient approximation made simple Interplay between crystalline chirality and magnetic structure in Mn1−xFexSi Crystal structure and thermal expansion of Mn1−xFexGe Dzyaloshinskii-moriya interaction as a consequence of a doppler shift due to spin-orbit-induced intrinsic spin current Energy band structure calculations for crystals with spiral magnetic structure Density-functional theory and strong interactions: orbital ordering in Mott-Hubbard insulators Download references The neutron experiment at the Materials and Life Science Experimental Facility of J-PARC was performed under the user program (Proposal No This work was supported by JSPS KAKENHI (Grants No RIKEN Center for Emergent Matter Science (CEMS) Comprehensive Research Organization for Science and Society (CROSS) The Institute for Solid State Physics (ISSP) synthesized polycrystalline samples of MnSi1−xGex and performed magnetization and transport measurement by using PPMS/MPMS Neutron scattering experiment were performed by Y.F. Magnetization and Hall resistivity measurement under high magnetic field was performed by Y.F. Band structure calculations were performed by T.K All the authors discussed the results and commented on the manuscript The authors declare no competing interests Journal peer review information: Nature Communications thanks the anonymous reviewers for their contribution to the peer review of this work Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41467-019-08985-6 Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily. "none"); */ } $("document").ready(function($) { let url = window.location.pathname; let nav_home = $('.only-home'); if (url != '/' && url != '/#') nav_home.removeClass("row"); }); $("document").ready(function($) { let url = window.location.pathname; let nav_home = $('.only-home'); let nav_home2 = $('.only-home2'); let nav = $('.without-home'); if (url != '') { $(window).bind('scroll load resize' RORZE CORPORATION Yoshiyuki Fujishiro was appointed the president and CEO of RORZE Corporation the leading automation solutions provider in the semiconductor industry Mr.Fujishiro  previously served as executive director and general manager of software solutions department for several years He has deep knowledge and experience in the software and IT domain Fujishiro received his master degree in informatics from Kyoto University and an MBA degree from Kobe University A joyful evening may follow a sorrowful morning industrial and financial news about global economies with a focus on understanding them from within Please enable JS and disable any ad blocker Metrics details Hybrid cluster proteins (HCPs) are Fe-S-O cluster-containing metalloenzymes in three distinct classes (class I and II: monomer all of which structurally related to homodimeric Ni Here we show X-ray crystal structure of class III HCP from Methanothermobacter marburgensis (Mm HCP) demonstrating its homodimeric architecture structurally resembles those of CODHs despite the different architectures of class III and I/II HCPs [4Fe-4S] and hybrid clusters are found in equivalent positions in all HCPs Structural comparison of Mm HCP and CODHs unveils some distinct features such as the environments of their homodimeric interfaces and the active site metalloclusters structural analysis of Mm HCP C67Y and characterization of several Mm HCP variants with a Cys67 mutation reveal the significance of Cys67 in protein structure metallocluster binding and hydroxylamine reductase activity Structure-based bioinformatics analysis of HCPs and CODHs provides insights into the structural evolution of the HCP/CODH superfamily the metallocluster-binding sites of CODHs and HCPs are located at equivalent positions the Ni-Fe-S-type cluster (C-cluster) for CODHs and Fe-S-O-type cluster (hybrid cluster) for HCPs despite differences in their cluster types This indicates that CODHs and HCPs originate from the same ancestor and belong to the same protein superfamily: the HCP/CODH superfamily the structure-based evolutionary relationship between CODHs and HCPs remains unverified We herein studied X-ray crystal structure of class III Methanothermobacter marburgensis HCP (Mm HCP) for structural comparison of it with class I/II HCPs and CODHs to gain critical insights into the structural diversity and evolution of the CODH/HCP superfamily we studied several Mm HCP variants with a Cys67 mutation showing the N-terminal denaturation with keeping this homodimeric architecture Each polypeptide of Mm HCP is shown in green or light blue [4Fe-4S] cluster and hybrid cluster ([4Fe-2S-3O] cluster) are represented as spheres S and O atoms of the metallocentres are coloured in brown b View of the alignments of the metal cofactors of Mm HCP in the same orientation as the overall structure in Fig Dashed lines indicate the distances between metallocentres c Close-up view of the metal-binding sites of Mm HCP These metallocentres were symmetrically aligned from the dimer interface of Mm HCP (Fig. 1b) In one side of the aligned symmetric clusters the distances between the Fe site and [4Fe-4S] and [4Fe-4S] and hybrid clusters were reasonable for electron transfer between the metallocentres in one side the distance between two [4Fe-4S] clusters was longer than that of the metalloclusters (i.e [4Fe-4S] and hybrid cluster of the HCP domain) in one side suggesting that electron transfer in one side is more favourable than that between two HCP domains via the interface in the schematic primary structures indicate conserved Cys As an exceptional region showing no clear sequence similarity to the other regions of class III Mm HCP, a green-coloured loop–short–helix region (loop-short-helix repeat) was identified in class I Dv HCP (Fig. 2b, d) This region links one outer helix (in brown) and one inner helix (in cyan) indicating that it is unique to monomeric HCPs In the class I Dv HCP-based model of Pf HCP the residue Leu96-Met106 formed a long loop connecting two helices this loop was not observed in class III Mm HCP in the class III Mm HCP-based model of Pf HCP the residue Leu96-Met106 formed an α-helix corresponding to one of the inner helices at the dimer interface the X-ray crystal structure of class III HCP is important for developing more reliable structural models for other class III HCPs The blue and red coloured surfaces indicate the positively and negatively charged regions The white-coloured surface indicates the hydrophobic region The box in the thick black line indicates the close-up views around the N-terminal [4Fe-4S] cluster which could mainly serve as an electron-accepting cofactor from outside [4Fe-4S] and hybrid clusters are shown in spheres a physiological meaning of rubredoxin is unclear; however it will be of interest to consider a physiological partner of class III HCPs hybrid cluster and C-cluster are shown as spheres Residues at the interfaces are represented as stick models indicating that the interfacial Fe-S cluster is a distinct feature between class III HCPs and CODHs By viewing both the dimer interfaces and absence/presence of the interfacial Fe-S cluster we demonstrated that class III HCPs and CODHs may be evolutionarily more distinct than expected based on the overall structure Considering the equivalent positions of the ligands of HCPs/CODHs despite some differences in amino acid ligand types a hypothetical scenario for differences in the ligand sets may have been caused by point mutations during HCP/CODH family evolution a spontaneous point-mutated Mm hcp was unexpectedly cloned DNA sequencing revealed that this mutation was a Cys67 to Tyr change resulting in the codon conversion of TGC to TAC at residue 67 Although the exact reason for this mutation remains unclear; it may have been caused by directly cloning Mm hcp using M marburgensis cells (not its pure genomic DNA) as a template in polymerase chain reaction (PCR) which was modelled as a [4Fe-2S-3O] cluster None of the [4Fe-4S] clusters or the rubredoxin domain were modelled because there were no observations in the corresponding electron density maps b Hybrid cluster-binding site of the Mm HCP C67Y variant Ligands of the hybrid cluster are coloured in cyan The surrounding residues (W432–M433 and Q435–A437) are coloured in pink and Glu434 is coloured in green the C67Y mutation may have induced the loss of the [4Fe-4S] cluster in Mm HCP C67Y possibly causing N-terminal region denaturation the loop region around Glu434 became unstabilized and shifted to the solvent area which may have been favourable because the hydrophilic residues may compensate for the positions where the [4Fe-4S] cluster-binding domain of WT was occupied resulting in the stabilisation of this area via networks of polar interactions with solvent waters Spectroscopic and functional characterisations were performed to assess Mm HCP WT and the C67Y variant To determine the effects of Cys67 mutation on Mm HCP properties the C67S and C67A variants were prepared and characterised b EPR spectra of the as-isolated Mm HCP WT and variants in the magnetic fields 3300–3500G c EPR spectra of the dithionite-reduced Mm HCP WT and variants in the magnetic fields 3300–3700G C67S and C67A variants are indicated as black Inserted grey lines in the EPR spectra allow a comparison of the EPR signals and their g-values Mm HCP WT showed two clear EPR signals at g = 9.424 and 6.374 which can be assigned to the [4Fe-2S-3O]-type hybrid cluster as observed in previous data for the EPR of Pf HCP Mm HCP C67Y and C67A exhibited weak EPR signals further indicating that this variant has no hybrid cluster these variants are unlikely to have the [4Fe-4S] cluster supporting the significance of the Glu ligand for HCPs function The lowest kcat can be understood by the fact that this variant had neither [4Fe-4S] nor [4Fe-2S-3O]-type clusters unknown Fe-type cofactor may have been included in this variant as seen in EPR spectra which may cause the structure of Mm HCP C67A was rather different from WT and the other variants and lower KM It is noted that Mb CODH is a dimer of heterodimer (α- and ε-domains) but its catalytic core is composed of a homodimer of α-domains in the same manner as other homodimer-type CODHs we identified the X-ray crystal structure of class III HCPs using Mm HCP and demonstrated that its unique homodimeric architecture is related to CODHs providing structure-based clues for the hypothetical scenario of the molecular evolution of the HCP/CODH superfamily we analysed the Mm HCP variants with Cys67 mutation a ligand of the N-terminal [4Fe-4S] cluster and demonstrated the significance of the N-terminal region for the architecture around the hybrid cluster and catalytic function of HCPs Considering that three-dimensional structures of all HCP classes—class I HCPs from D they can be used in future structural comparison studies to provide insights regarding the diversity of HCP classes evolutionary relationships between HCPs and CODHs and interactions between HCPs and their potential partners at the molecular level The presented structure of class III HCP is a milestone in understanding the origin of the HCP/CODH superfamily a key enzyme superfamily in the anaerobic biological world Isopropyl-β-D-thiogalactopyranoside (IPTG) was purchased from BLD Pharmatech Inc Imidazole and dithiothreitol (DTT) were purchased from Wako Pure Chemical Industries (Osaka Other chemicals used in this study were purchased from Sigma-Aldrich (St All oligo DNA primers were purchased from Eurofins Genomics Inc marburgensis was provided by RIKEN BRC through the National BioResource Project of MEXT/AMED The M. marburgensis gene encoding Mm HCP was amplified via PCR using KOD FX neo (Toyobo, Japan), M. marburgensis cells and NdeI-Mm HCP-F and SalI-Mm HCP-R primers (Supplementary Table 1) The amplified gene was then cloned into the pET21a plasmid (Novagen The cloned genes were verified via DNA sequencing which may have been caused by a spontaneous point mutation during the PCR cycle it was hypothesised that this occurred because PCR amplification of Mm hcp was performed using a crude sample The expression plasmids for Mm HCP WT and Mm HCP C67Y variants were designated as pET21a-mmhcp-his and pET21a-mmhcp-c67y-his To construct the expression plasmids for the rubredoxin domain, the Mm HCP C67S variant and Mm HCP C67A variant, inverse PCR was performed using pET21a-mmhcp-his as a template and the mutagenic primers listed in Supplementary Table 1 The amplified PCR products were treated with DpnI at 37 °C for 2 h The DpnI-treated DNA fragments were ligated and transformed into E The colonies of the transformants were cultivated and plasmids were extracted pET21a-mmhcp-c67s-his and pET21a-mmhcp-c67a-his) were verified via DNA sequencing coli cells were then cultured at 37 °C for 4 h in Luria–Bertani (LB) medium supplemented with 100 μg/mL ammonium iron citrate 50 μg/mL ampicillin and 20 μg/mL gentamycin When the optical density at 600 nm (OD600) reached 0.8–1.0 ITPG was added to the culture at a final concentration of 1 mM The culture was then sealed under anaerobic conditions followed by further cultivation at 20 °C for 20 h the cells were harvested by centrifuging the samples at 4 °C and 9000 × g for 20 min The harvested cells were frozen in liquid nitrogen and stored at −80 °C until further use coli cells were resuspended in buffer A (50 mM Tris–HCl buffer disrupted by sonication on ice and then centrifuged at 4 °C and 20,000 × g for 40 min The resulting supernatant was loaded onto a HisTrap FF crude column (Cytiva proteins bound to the column were eluted with buffer B (50 mM Tris–HCl buffer The eluted fractions were concentrated using a 50-kDa cutoff Amicon Ultra-15 (Merck KGaA The concentrated fractions were then loaded onto a Sephacryl S-200 16/60 gel filtration column (Cytiva) equilibrated with buffer C (50 mM Tris–HCl The Mm HCP fractions were then pooled and concentrated for further use coli C41(DE3) cells transformed with the pET21a-rubredoxin plasmid were cultured at 37 °C for 4 h in LB medium supplemented with 100 μg/mL ammonium iron citrate and 50 μg/mL ampicillin IPTG was added to the culture at a final concentration of 1 mM once the optical density at 600 nm (OD600) reached 0.8–1.0 The cells were subsequently cultured at 20 °C for 20 h and harvested by centrifugation at 4 °C and 9000 × g for 20 min The harvested cells were then frozen in liquid nitrogen and stored at −80 °C until further use The rubredoxin domain was aerobically purified at 4 °C or on ice coli cells expressing the rubredoxin domain were disrupted by sonication on ice and then centrifuged at 4 °C and 20,000 × g for 40 min for preparation of the supernatant the supernatant was heated at 65 °C for 20 min and then centrifuged in the same conditions as described above the resulting supernatant was concentrated using a 3-kDa cutoff Amicon Ultra-15 (Merck KGaA) The concentrated rubredoxin domain-containing solution was loaded onto a Sephacryl S-100 16/60 gel column (Cytiva) and equilibrated with buffer C Red-brown fractions containing the rubredoxin domain were pooled and concentrated for further use UV–visible spectra of Mm HCP WT and the variants in as-isolated and dithionite-reduced forms were anaerobically recorded in the Coy chamber The dithionite-reduced forms of Mm HCP WT and the variants were prepared by adding 1 mM sodium dithionite to the as-isolated Mm HCP WT and different variants Protein concentration of each of Mm HCP WT and the variants for UV–visible spectroscopy was 40 μM The UV–visible spectrum of the rubredoxin domain was aerobically recorded Protein concentration of the rubredoxin domain for UV–visible spectroscopy was 14 μM a NanoPhotometer C40 UV–visible spectrophotometer was used with a quartz cuvette with a 1 cm path Mm HCP C67S variant and Mm HCP C67A variant in the as-isolated forms were recorded on a Bruker E500 (Bruker) equipped with an Oxford ESR900 cryostat (Oxford Instruments The EPR spectra of the reduced states of Mm HCP WT Mm HCP C67S variant and Mm HCP C67A variant with 10 mM sodium dithionite were recorded at 12 K Protein concentrations were 4 mM for WT and 2 mM for the variants in both as-isolated and dithionite-reduced states The EPR spectrum of the rubredoxin domain in an as-isolated state was recorded at 20 K The following parameters were used for EPR measurements: microwave frequency Protein concentration of the as-isolated rubredoxin domain for EPR measurement was 0.28 mM The initial rate of this consumption was plotted against the concentrations of hydroxylamine as a substrate The plots were used for non-linear least squares curve-fitting using the Michaelis–Menten equation resulting in the calculation of kcat and KM values Curve-fitting was performed using Igor Pro 8.0 software (WaveMetrics The reactions at each time point were conducted in at least triplicate (n = 3) for each reaction composition Crystals of WT and the C67Y Mm HCP variant were obtained within 3 months the inner pressure of the anaerobic Coy chamber was partially reduced via vacuuming to avoid blowout of the chamber due to vaporising liquid nitrogen A crystal of interest was picked up with a cryo-loop (CrystalCap SPINE HT followed by immersing the crystal into the liquid nitrogen as soon as possible a plastic cap was attached to the crystal-mounted loop in liquid nitrogen the crystal in liquid nitrogen in the dewar flask was taken out from the anaerobic chamber Superimposition of protein structures was conducted in PyMOL Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article Structure and function of the hybrid cluster protein Novel electron-paramagnetic resonance signals from an Fe/S protein containing 6 iron atoms Purification and biochemical characterization of a putative [6Fe-6S] prismane-cluster-containing protein from Desulfovibrio vulgaris (Hildenborough) Direct spectroscopic evidence for the presence of a 6Fe cluster in an iron–sulfur protein isolated from Desulfovibrio desulfuricans (ATCC 27774) EPR spectroscopy of putative enzyme intermediates in the NO reductase and the auto-nitrosylation reaction of Desulfovibrio vulgaris hybrid cluster protein in high affinity nitric oxide reduction that protects anaerobic cultures of Escherichia coli against nitrosative stress The hybrid-cluster protein (‘prismane protein’) from Escherichia coli Characterization of the hybrid-cluster protein redox properties of the [2Fe-2S] and [4Fe-2S-2O] clusters and identification of an associated NADH oxidoreductase containing FAD and [2Fe-2S] Hydroxylamine reductase activity of the hybrid cluster protein from Escherichia coli Modulation of virulence by two acidified nitrite-responsive loci of Salmonella enterica serovar Typhimurium Identification of the hybrid cluster protein from amitochondriate eukaryotes and its phylogenetic implications Hydroxylamine assimilation by Rhodobacter capsulatus E1F1 Requirement of the hcp gene (hybrid cluster protein) located in the nitrate assimilation nas gene region for hydroxylamine reduction Dissimilatory metabolism of nitrogen oxides in bacteria: comparative reconstruction of transcriptional networks The role of the hybrid cluster protein in oxidative stress defense The NsrR regulon in nitrosative stress resistance of Salmonella enterica serovar Typhimurium A multiplex enzymatic machinery for cellular protein S-nitrosylation The “prismane” protein resolved: X-ray structure at 1.7 Å and multiple spectroscopy of two novel 4Fe clusters Hybrid-cluster protein (HCP) from Desulfovibrio vulgaris (Hildenborough) at 1.6 Å resolution Hybrid cluster proteins (HCPs) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at 1.25 Å resolution using synchrotron radiation Structure of the hybrid cluster protein (HCP) from Desulfovibrio desulfuricans ATCC 27774 containing molecules in the oxidized and reduced states Reduced hybrid cluster proteins (HCP) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at high resolution using synchrotron radiation Structural and functional relationships in the hybrid cluster protein family: structure of the anaerobically purified hybrid cluster protein from Desulfovibrio vulgaris at 1.35 Å resolution Structural and biochemical elucidation of class I hybrid cluster protein natively extracted from a marine methanogenic archaeon Crystal structure of Escherichia coli class II hybrid cluster protein reveals a [4Fe-4S] cluster at the N-terminal protrusion Escherichia coli class II hybrid cluster protein HCP in Encyclopedia of Inorganic and Bioinorganic Chemistry A thermostable hybrid cluster protein from Pyrococcus furiosus: effects of the loss of a three helix bundle subdomain and mechanism of the nickel metalloenzymes Maturation of the [Ni–4Fe–4S] active site of carbon monoxide dehydrogenases Evolutionary history of carbon monoxide dehydrogenase/acetyl-CoA synthase Structural and phylogenetic diversity of anaerobic carbon-monoxide dehydrogenases Gas channel rerouting in a primordial enzyme: structural insights of the carbon-monoxide dehydrogenase/acetyl-CoA synthase complex from the acetogen Clostridium autoethanogenum Life on carbon monoxide: X-ray structure of Rhodospirillum rubrum Ni-Fe-S carbon monoxide dehydrogenase A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase Ni-Zn-[Fe4-S4] and Ni-Ni-[Fe4-S4] clusters in closed and open α subunits of acetyl-CoA synthase/carbon monoxide dehydrogenase Carbon monoxide induced decomposition of the active site [Ni−4Fe−5S] cluster of CO dehydrogenase Carbon dioxide activation at the Ni,Fe-cluster of anaerobic carbon monoxide dehydrogenase Xenon in and at the end of the tunnel of bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase Structure of the α2ε2 Ni-dependent CO dehydrogenase component of the Methanosarcina barkeri acetyl-CoA decarbonylase/synthase complex Structural basis of cyanide inhibition of Ni Fe-containing carbon monoxide dehydrogenase Crystallographic snapshots of cyanide- and water-bound C-clusters from bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase n-Butyl isocyanide oxidation at the [NiFe4S4OHx] cluster of CO dehydrogenase How the [NiFe4S4] cluster of CO dehydrogenase activates CO2 and NCO- When the inhibitor tells more than the substrate: the cyanide-bound state of a carbon monoxide dehydrogenase CODH-IV: A high-efficiency CO-scavenging CO dehydrogenase with resistance to O2 Redox-dependent rearrangements of the NiFeS cluster of carbon monoxide dehydrogenase Structural insight into metallocofactor maturation in carbon monoxide dehydrogenase Crystallographic characterization of the carbonylated A-cluster in carbon monoxide dehydrogenase/acetyl-CoA synthase The two CO-dehydrogenases of Thermococcus sp A morphing [4Fe-3S-nO]-cluster within a carbon monoxide dehydrogenase scaffold Visualizing the gas channel of a monofunctional carbon monoxide dehydrogenase Crystal structure of rubredoxin from Pyrococcus furiosus at 0.95 Å resolution and the structures of N-terminal methionine and formylmethionine variants of Pf Rd Contributions of N-terminal interactions to thermostability Crystallographic refinement of rubredoxin at 1.2 Å resolution EPR spectroscopy of biological iron-sulfur clusters with spin-admixed S = 3/2 ground states PROMALS3D: a tool for multiple protein sequence and structure alignments Visualizing the superfamily of metallo-β-lactamases through sequence similarity network neighborhood connectivity analysis The structure of the dimeric state of IscU harboring two adjacent [2Fe-2S] clusters provides mechanistic insights into cluster conversion to [4Fe-4S] Hyperproduction of recombinant ferredoxins in Escherichia coli by coexpression of the ORF1-ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster Coot: model-building tools for molecular graphics Refinement of macromolecular structures by the maximum-likelihood method Towards automated crystallographic structure refinement with phenix.refine Use of knowledge-based restraints in phenix.refine to improve macromolecular refinement at low resolution MolProbity: all-atom structure validation for macromolecular crystallography Clustal Omega for making accurate alignments of many protein sequences Deciphering key features in protein structures with the new ENDscript server PDBsum: structural summaries of PDB entries DALI shines a light on remote homologs: one hundred discoveries MEGA11: molecular evolutionary genetics analysis version 11 SWISS-MODEL: homology modelling of protein structures and complexes Electrostatics of nanosystems: application to microtubules and the ribosome Download references Yasuhiro Takahashi at Saitama University (Saitama The authors are grateful to the staff of Photon Factory (Tsukuba Japan) and SPring-8 for the use of beamlines with proposals 2018G505 The use of the beamlines for X-ray crystallography was also partially supported by the Platform Project for Supporting Drug Discovery and Life Science Research BINDS from AMED as well as by the Cooperative Research Program of the Institute for Protein Research Mizue Asada for their help with the EPR measurements at the Institute for Molecular Science (Okazaki supported by the Nanotechnology Platform Program This work was financially supported by JSPS Grant-in-Aid for Transformative Research Areas JSPS Grant-in-Aid for Scientific Research (C) 22K05137 (to T.F.) and Tokyo Chemical Industry Foundation (to T.F.) Department of Biochemistry and Molecular Biology Graduate School of Science and Engineering spectroscopic measurements and bioinformatics; T.F Nature Communications thanks Peter-Leon Hagedoorn reviewer(s) for their contribution to the peer review of this work Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41467-023-41289-4 a shareable link is not currently available for this article Sign up for the Nature Briefing newsletter — what matters in science The all-Japanese final between Kisumi Omori and Kokoro Fujishiro delivered a tough gripping battle combined with a display of varied and dangerous ne-waza Both came close to scoring on the ground but in tachi-waza they could not be separated at all It took more than 8 minutes of fighting for Omori to come out on top It was a tactical win between two skilled judoka The first bronze medal contest saw Ayumi Leiva Sanchez (ESP) face Sofia Asvesta (CYP) both of whom had given electric performances through the morning session For the first 2 and a half minutes there was really nothing between them but then Leiva Sanchez took a deep cross grip over the back and applied a strong o-uchi-gari which It scored ippon and gave Spain their first medal of this Paris Grand Slam In the second bronze medal contest of the -52 kg category Khorloodoi Bishrelt (UAE) and Distria Krasniqi (KOS) faced off The -48 kg Olympic champion in Tokyo and Olympic silver medallist at -52 kg last year won the fight by capitalising on a poorly prepared seoi-otoshi from Bishrelt pulling her back for a clean landing on the back Krasniqi didn’t look happy with the overall result of the day but could be satisfied with still reaching the podium 2025 / On Monday 14th April 2025 the International Judo Federation .. 2025 / Daniel De Angelis is the Director of the IJF Kata Commission 2025 / Follow the Reims IJF Kata World Series 2025 live on .. 2025 / The Tbilisi Grand Slam is a firm favourite on the IJF .. 2025 / Catherine 'Cathy' Arnaud is a legend of French and .. 2025 / Marie-Paule Panza is a figure of judo in the East of .. 2025 / Daniel Fernandes (FRA) could be found all weekend around .. Metrics details The electrical Hall effect can be significantly enhanced through the interplay of the conduction electrons with magnetism which is known as the anomalous Hall effect (AHE) Whereas the mechanism related to band topology has been intensively studied towards energy efficient electronics those related to electron scattering have received limited attention Here we report the observation of giant AHE of electron-scattering origin in a chiral magnet MnGe thin film reach \(40,000\) Ω−1 cm−1 and \(18\)% in the ferromagnetic region exceeding the conventional limits of AHE of intrinsic and extrinsic origins A possible origin of the large AHE is attributed to a new type of skew-scattering via thermally excited spin-clusters with scalar spin chirality which is corroborated by the temperature–magnetic-field profile of the AHE being sensitive to the film-thickness or magneto-crystalline anisotropy Our results may open up a new platform to explore giant AHE responses in various systems including frustrated magnets and thin-film heterostructures the Hall conductivity of these “intrinsic” mechanisms has the upper threshold set by the Berry curvature In the case of momentum space Berry curvature the consequent Hall conductivity should be less than e2/ha (h and a being Planck’s constant and a typical lattice constant values) and hence of the order of \(\sigma _{xy} = 10^2\)–\(10^3\) Ω−1 cm−1 a B20-type chiral crystal structure (space group P213) of MnGe The chirality is characterized by the stacking direction of atoms as viewed from [111] direction b Temperature dependence of conductivity (\(\sigma _{xx}\)) at zero magnetic field for the film-thickness of t = 160 nm c Magnetic-field dependence of Hall conductivity (\(\sigma _{xy}\)) at various temperatures for t = 160 nm The negative dip structures are attributed to topological Hall effect arising from the formation of spin hedgehogs and anti-hedgehogs bridged by skyrmion-strings (shown in the inset) d High-magnetic-field data of \(\sigma _{xy}\) at various temperatures for t = 160 nm The value of the quantization limit (\(e^2/ha\)) in three-dimensions is denoted by a dashed line The observed giant AHE far exceeds the value allowed by the intrinsic AHE the reason why the Hall signal is enhanced at high magnetic field under fixed temperature remains elusive and needs further theoretical investigations This may be attributed to the divergent spin correlation and the criticality around the FM transition The former invalidates the short-correlation-length assumption used in the skew-scattering theory while the latter may affect the transport phenomena in a nontrivial way Study on such effects is interesting but requires intensive theoretical investigations that go beyond the scope of this paper Contour plot of \(\tan \Theta _{\mathrm{H}}( = \sigma _{xy}/\sigma _{xx})\) for the thickness of \(t = 80\) (a) The solid diamond markers connected by a dashed line represent the maximum point of \(\tan \theta _{\mathrm{H}}\) at each temperature The insets are the intuitive schematics to explain the fact that the larger scalar spin chirality excitation is more favorable in thinner films with enhanced in-plane magnetic anisotropy and the three-spin correlation without SOC should dominate over the two-spin correlation with SOC Further experiments such as the direct observation of electronic structure would be necessary to discuss the possibility of large normal Hall effect or the presence of magnetic Weyl points in MnGe the characteristic features discussed in this work and magneto-crystalline anisotropy dependence of the Hall response strongly suggest the spin-chirality skew-scattering mechanism argued above Unlike the conventional intrinsic AHE which has been limited to ferromagnets or the ordered phases of the SSC the proposed new paradigm of spin-chirality skew-scattering would be applicable to a wide range of materials; since it is expected even in materials with no magnetic-ordering as well as in the temperature-magnetic-field region where the SSC loses its long-range order from a perspective of short-range spin correlation would open up a new frontier in the discovery of novel electronic functionalities MnGe thin films were grown epitaxially on Si(111) substrates by the molecular beam epitaxy method22 We employed a 2 nm thick MnSi(111) buffer layer which was grown by reacting a deposited Mn layer with the Si(111)-(7\(\times\)7) surface at \(250\,^\circ {\mathrm{C}}\) Mn and Ge were co-evaporated at approximately \(90\,^\circ {\mathrm{C}}\) followed by annealing at \(250\,^\circ {\mathrm{C}}\) The growth of B20-type MnGe was confirmed by \(\theta\)-\(2\theta\) x-ray diffraction scans Resistivities up to 14 T were measured with a conventional four-probe method using a DC option of Physical Properties Measurement System (PPMS High-magnetic-field transport measurements up to 56 T were performed utilizing non-destructive pulse magnets energized by capacitor banks installed at the International MegaGauss Science Laboratory of Institute for Solid State Physics (ISSP) The magnetic field was applied perpendicular to the film plane while the electric field was applied parallel to \(\left[ {1\bar 10} \right]\) direction The longitudinal conductivity (\(\sigma _{xx}\)) and the Hall conductivity (\(\sigma _{xy}\)) were calculated as \(\sigma _{xx} = \rho _{xx}/( {\rho _{xx}^2 + \rho _{yx}^2} )\) and \(\sigma _{xy} = \rho _{yx}/(\rho _{xx}^2 + \rho _{yx}^2)\) \(\rho _{xx}\) and \(\rho _{yx}\) are the longitudinal and Hall resistivity We could not perform the transport measurements in thick samples (film-thickness larger than \(400\)  nm) due to the formation of microcracks upon cooling The spots were isolated using the objective aperture and the passing electrons were focused into an image of the domains onto the charge-coupled device (CCD) camera installed within the microscope The data sets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request Berry phase effects on electronic properties The anomalous Hall effect and magnetic monopoles in momentum space Large anomalous Hall effect in a half-Heusler antiferromagnet Giant anomalous Hall effect in a ferromagnetic Kagomé-lattice semimetal Experimental observation of the quantum anomalous Hall effect in a magnetic topological insulator Hall effect of the colossal magnetoresistance manganite La1-xCaxMnO3 Berry phase theory of the anomalous Hall effect: application to colossal magnetoresistance manganites and anomalous Hall effect in a frustrated ferromagnet Topological Hall effect in the A phase of MnSi Large topological Hall effect in a short-period helimagnet MnGe Skyrmion lattice with a giant topological Hall effect in a frustrated triangular-lattice magnet Crossover behavior of the anomalous Hall effect and anomalous Nernst effect in itinerant ferromagnets Quantum transport theory of anomalous electric The spontaneous Hall effect in ferromagnetics II Side-jump mechanism for the Hall effect of ferromagnets Extrinsic anomalous Hall effect in charge and heat transport in pure iron Fe0.997Si0.003 Multivariable scaling for the anomalous Hall effect Topological spin-hedgehog crystals of a chiral magnet as engineered with magnetic anisotropy Engineering skyrmions and emergent monopoles in topological spin crystals Repicky, J. et al. Atomic scale visualization of topological spin textures in the chiral magnet MnGe. Preprint at https://arxiv.org/abs/2008.00886 (2020) Ishizuka, H. & Nagaosa, N. Theory of giant skew scattering by spin cluster. Preprint at https://arxiv.org/abs/1906.06501 (2020) unconventional anomalous Hall effect in the metallic frustrated magnet candidate Multiple-q states and the skyrmion lattice of the triangular-lattice Heisenberg antiferromagnet under magnetic fields Topological-chiral magnetic interactions driven by emergent orbital magnetism Anomalous Hall conductivity due to vector spin chirality in the weak coupling regime Chiral Hall effect in noncollinear magnets from a cyclic cohomology approach Noncommutative quantum mechanics and skew scattering in ferromagnetic metals Ultrahigh mobility and giant magnetoresistance in the Dirac semimetal Cd3As2 Twin domains in epitaxial thin MnSi layers on Si(111) Determination of crystallographic chirality of MnSi thin film grown on Si(111) substrate Large anomalous Hall effect in a non-collinear antiferromagnet at room temperature Large anomalous Hall effect in a silicon-based magnetic semiconductor Anomalous Hall effect derived from multiple Weyl nodes in high-mobility EuTiO3 films Download references Mogi for experimental supports and fruitful discussions This research was supported in part by JSPS KAKENHI (Grants No These authors contributed equally: Yukako Fujishiro Transport and magnetization measurements using PPMS/MPMS were performed by Y.F High-magnetic-field transport measurements were performed by Y.F. Theoretical calculations were provided by H.I Dark-field TEM measurements were performed by F.S.Y wrote the paper with the support from H.I. Peer review information Nature Communications thanks the anonymous reviewers for their contribution to the peer review of this work Download citation DOI: https://doi.org/10.1038/s41467-020-20384-w Metrics details Rice grain number directly affects crop yield Identifying alleles that improve panicle architecture would greatly aid the development of high-yield varieties we show that the quantitative trait locus qSrn7 contains rice FRIZZY PANICLE (FZP) a previously reported gene encoding an ERF transcription factor that promotes floral transition Reduced expression of FZP in the reproductive stage increases the extent of higher order branching of the panicle Genotype analysis of this gene in cultivars from the publicly available National Institute of Agrobiological Sciences (NIAS) Core Collection demonstrated that the extent of higher order branching was increased in those cultivars carrying the FZP allele associated with qSrn7 chromosome segment substitution lines resulting from a cross between Koshihikari and Kasalath also showed that upper panicle higher order branching and grain yield were increased by qSrn7/FZP Our findings indicate that qSrn7/FZP influences panicle branching pattern and is thus useful in the breeding of high-yield rice varieties Panicle branching pattern of Koshihikari and ST-5 (b) Panicle morphologies of Koshihikari and ST-5 Red circles indicate secondary rachis branches (SBs) (c–g) Comparison of panicle attributes between Koshihikari and ST-5 (d) Number of primary rachis branches (PBs) per main panicle (f) Number of tertiary branches per main panicle (g) Comparison of number of SBs per PBs between Koshihikari and ST-5 (h) Panicle branching pattern of Koshihikari and ST-5 **Significant at 1% level (Student’s t-test) These past studies have examined terminal phenotypes such as grain number and panicle length; however panicle structure as determined by its branching pattern has not been characterized in detail such as through comprehensive analysis of several organs The rice panicle exhibits a recursive structure in which higher-order rachis branches radiate from lower-order rachis branches knowledge of how the order of each rachis branch is affected by a particular gene will greatly assist in efforts to alter the architecture of the rachis for optimal yield identifying a novel quantitative trait locus (QTL) that regulates these organs would assist in breeding new rice varieties with increased yields we describe the identification and characterization of a major QTL which regulates a novel panicle branching pattern in rice Analysis of chromosome segment substitution lines (CSSLs) carrying qSrn7 showed that grain number and yield increased in conjunction with changing panicle structure and branching pattern These results describe the mechanism by which qSrn7 influences panicle structure and suggest that qSrn7 may be used in efforts to generate rice varieties with high grain yield Aanalysis of FZP expression and FZP antisense transgenic phenotype (a) The qSrn7 locus was detected between RM22060 and RM22156 on Chromosome 7 (b) Numbers on the map indicate the number of recombinants (c) The candidate region of qSrn7 was located to the 35.3-kb region between RM22114 and RM22118 by fine mapping Nucleotide insertions present in ST-5 are indicated (e) FZP expression analysis in inflorescence tissue at various developmental stages and in leaf tissue by quantitative real-time PCR PBI: Primary rachis branch initiation stage SI: Spikelet initiation stage of inflorescence development (f) Comparison of number of SBs per main panicle between vector control and FZP antisense transgenic plants (g) In situ hybridization of FZP transcript during panicle development in Koshihikari (A,C) and ST-5 (B,D) (A–D) Developing inflorescence at the stage of primary (A,B) and secondary (C,D) branch differentiation (Insets) Close-up view of the apical region of a developing primary branch that is initiating a secondary rachis branch meristem Arrowheads indicate incidence region of expression *Significant at 5% level (Student’s t-test) (a) Dot plots of total SB number per panicle against the genotype determined at RM22114 and RM22115 (b) Dot plots of SB number per upper three PBs of the panicle against the genotype determined at RM22114 and RM22115 (c) Panicle branching patterns in lines carrying ST-5 (top panels) and Koshihikari (bottom panels) genotypes determined at RM22114 and RM22115 (d) Panicle branching pattern of Koshihikari Red circles: secondary rachis branches (SBs) (e) Comparison of panicle branching patterns of Koshihikari Tln: Total number of lateral branches on the PB (SBs + SRs) Solid lines denote the loess smoothing curves and shadows represent the estimated SE (f) Relative expression of FZP in Koshihikari and CSSLs in developing inflorescences at the secondary branch initiation stage These results suggest that the qSrn7/FZP allele has reduced expression in tissue at the SB initiation stage which causes an increase in higher order branching in upper regions of the panicle by preventing the transition from branch to spikelet meristem Grain yield performance of CSSLs compared with Koshihikari (c–g) Comparison of main panicle traits between Koshihikari (d) Number of primary rachis branches (PBs) per panicle (e) Number of secondary rachis branches (SBs) per panicle (f) Total number of lateral branches (secondary rachillae (SRs) + SBs) per panicle (h–j) Comparison of yield factors between Koshihikari **P < 0.01; *P < 0.05 versus Koshihikari (Dunnett’s multiple comparison test) it is important to characterize how a particular gene affects the panicle branching pattern Efforts to improve panicle grain number would benefit both from focusing on previously characterized genes that influence branching pattern and on identifying additional genes that influence panicle traits genes that affect panicle branching pattern had not hitherto been identified we used a rice variety with increased panicle grain number to identify and characterize a gene involved in panicle branching pattern suggesting that rice qSrn7/FZP cultivars may benefit rice breeding projects aimed at improving grain yield These results show that qSrn7/FZP affects higher order branching and suggests that combining qSrn7/FZP with other genes affecting panicle structure would be a beneficial strategy in projects aimed at developing novel high-yield rice varieties this study demonstrates that the combination of QTL analysis using natural variation and the detailed analysis of panicle branching pattern is a useful strategy for identifying genes that can further increase crop productivity Experimental plant material was grown in the research field of Togo Field for Science and Education at Nagoya University Field experiments for the grain yield were performed for two years therefore the cultivation results for 2015 are indicated here The transgenic plants were grown in isolated greenhouses under standard growth conditions Main panicle per plant was used for analysis of panicle branching pattern Measured parameters were the number of grains QTL analysis was performed with 94 F2 plants and the software package R/QTL (R version 3.1.3; R/qtl package 1.31–5). QTLs were identified using Haley–Knott regression and the significance threshold was set using 1,000 permutations. SSR markers used in positional cloning are listed in Supplementary Table 2 Transformed cells and plants were selected by hygromycin resistance and regenerated seedlings were grown to maturity in pots under greenhouse conditions More than ten independent T0 plants were isolated and five plants were analyzed transformants containing an empty vector were included and five of the resulting control plants were included in each analysis Total RNA from various organs was prepared as described by Sambrook et al.29 with some modifications and was treated with DNase I First-strand complementary DNA (cDNA) was synthesized from 1 μg of total RNA using a QuantiTect Reverse Transcription Kit (Qiagen) Quantitative real-time PCR was performed using the QuantiTect SYBR Green PCR Kit (Qiagen) and a LightCycler System (LightCycler 1.5; Roche Applied Science) Transcript levels were measured in three independent biological replicates The UBQ gene from rice was used as an internal standard for normalizing variations in cDNA concentration The primer sets used for PCR were as follows: FZP primers 5′-CACATTGGCTCGTACGGTC-3′ and 5′-GAGAAGAGGAAGTCGTGG-3′; UBQ primers 5′-AGAAGGAGTCCACCCTCCACC-3′ and 5′-GCATCCAGCACAGTAAAACACG-3′ Plant science called up to provide food security Architectural Evolution and its Implications for Domestication in Grasses Analysis of rice panicle traits and detection of QTLs using an image analyzing method Aberrant Panicle Organization 1 temporally regulates meristem identity in rice Expression level of ABERRANT PANICLE ORGANIZATION1 determines rice inflorescence form through control of cell proliferation in the meristem Genetical Studies on Dense and Lax Panicles in Rice: I Character Expression and Mode of Lax Panicle Rice Two-Step Regulation of LAX PANICLE1 Protein Accumulation in Axillary Meristem Formation in Rice Natural variation at the DEP1 locus enhances grain yield in rice Cytokinin oxidase regulates rice grain production OsSPL14 promotes panicle branching and higher grain productivity in rice Varietal Differences of Rice in Differentiation and Degeneration of Secondary Rachis-branches and Spikelets in Terms of Their Nodal Distribution on a Rachis Evaluation of yield performance in rice near-isogenic lines with increased spikelet number FRIZZY PANICLE is required to prevent the formation of axillary meristems and to establish floral meristem identity in rice spikelets Regulatory role of FZP in the determination of panicle branching and spikelet formation in rice Construction and Evaluation of Chromosome Segment Substitution Lines Carrying Overlapping Chromosome Segments of indica Rice Cultivar ‘Kasalath’ in a Genetic Background of japonica Elite Cultivar ‘Koshihikari’ Genome-wide association and high-resolution phenotyping link Oryza sativa panicle traits to numerous trait-specific QTL clusters Yield Potential Trends of Tropical Rice since the Release of IR8 and the Challenge of Increasing Rice Yield Potential Progress in ideotype breeding to increase rice yield potential The Control of Spikelet Meristem Identity by the branched silkless1 Gene in Maize Upf1 Senses 3′UTR Length to Potentiate mRNA Decay Nonsense-Mediated Decay of Alternative Precursor mRNA Splicing Variants Is a Major Determinant of the Arabidopsis Steady State Transcriptome The morphogenetic mechanism of formation of the panicle branching system in rice plants A high-efficiency Agrobacterium-mediated transformation system of rice (Oryza sativa L.) (New York: Cold spring harbor laboratory press Use of a new adhesive film for the preparation of multi-purpose fresh-frozen sections from hard tissues Isolation and characterization of novel nodulin cDNAs representing genes expressed at early stages of soybean nodule development Download references Sato for helping to analyze panicle branching pattern This work was supported by a grant from the Ministry of Agriculture Forestry and Fisheries of Japan (Genomics-based Technology for Agricultural Improvement Ayumi Agata and Sadayuki Ota contributed equally Graduate School of Bioagricultural Sciences performed the positional cloning experiments; Y.F. Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41598-018-30395-9 Metrics details Quantum states characterized by nontrivial topology produce interesting electrodynamics and versatile electronic functionalities One source for such remarkable phenomena is emergent electromagnetic field which is the outcome of interplay between topological spin structures with scalar spin chirality and conduction electrons it has scarcely been exploited for emergent function related to heat-electricity conversion Here we report an unusually enhanced thermopower by application of magnetic field in MnGe hosting topological spin textures By considering all conceivable origins through quantitative investigations of electronic structures and properties a possible origin of large magneto-thermopower is assigned to the strong energy dependence of charge-transport lifetime caused by unconventional carrier scattering via the dynamics of emergent magnetic field high-magnetic-field measurements corroborate the presence of residual magnetic fluctuations even in the nominally ferromagnetic region leading to a subsisting behavior of field-enhanced thermopower The present finding may pave a way for thermoelectric function of topological magnets High-performance thermoelectric materials provide a viable solution towards environmental issues since they realize efficient electricity generation from waste heat without greenhouse gas emissions1 extensive efforts have been made to enhance Seebeck coefficient (S) with minimal increase in electrical resistivity (ρ) to improve thermoelectric figure of merit ZT = S2T/ρκ where T and κ represent temperature and thermal conductivity Schematics of spin textures and magneto-thermopower in MnGe and MnSi a Spin texture of MnGe which can be regarded as a periodic array of hedgehogs and anti-hedgehogs with a short magnetic period of 3 nm They serve as quantized source (monopole) and sink (anti-monopole) of emergent magnetic field b Field-induced large magneto-thermopower observed in MnGe Topological phase transition from hedgehog lattice to ferromagnetic state manifests itself as a kink in the S–H curve (shown as black triangles) suggestive of its link to the observed thermopower There are also observed anomalies in the S–H curves at low temperatures c Magneto-thermopower profiles for MnGe in the temperature–magnetic field space The contour mappings are displayed in the bottom plane with the white line representing the phase boundary between ferromagnetic (FM) state and hedgehog lattice (HL) d Conical spin texture (C) with a magnetic period of 18 nm in MnSi e Magneto-thermopower in MnSi as the contrasting example to MnGe which shows a monotonic decrease generically found in magnetic materials f Magneto-thermopower profiles for MnSi in the temperature–magnetic field space which is revealed by photoemission spectroscopy (PES) and band calculations which alone is not enough to explain the observed magnitude Along with other striking contrast between magneto-resistivity (MR) and specific heat of MnGe and MnSi a unique scattering mechanism originating from strong fluctuations of emergent fields in MnGe may cause the strong dependence of transport lifetime leading to the enhanced S even at low temperatures This proposed scenario is corroborated by high-magnetic-field measurements where we demonstrate the close correlation between MR and S in terms of magnetic fluctuations which is a behavior generically expected for magnetic materials; this highlights the unconventional magneto-thermopower in MnGe Specific heat and magneto-resistivity in MnGe and MnSi Specific heat and magneto-resistivity for MnSi (a b C/T plotted against T2 under various magnetic fields d Change in the specific heat ΔC(H) as a function of external magnetic field normalized by the critical field Hc at respective temperatures MnGe shows a sharp enhancement at the topological phase transition (H/Hc = 1.0) f Magneto-resistivity normalized by the value at zero field at respective temperature Large positive anomaly observed in MnGe (f) is attributed to the fluctuations of emergent magnetic field (monopole fluctuations) which is again quantitatively insufficient to be a dominant origin of the large magneto-thermopower in MnGe The anticipated strong energy dependence of τ may also be rooted in such a H-dependent enhancement of the fluctuating emergent magnetic field Magneto-resistivity and thermopower in MnGe at high-magnetic fields a–d Longitudinal magneto-resistivity (MR) measured by pulsed magnet at low temperatures (T = 2 Thick-line curves are the results on the same sample by steady-field measurements (PPMS) up to 14 T Bold black curves are the estimated conventional MR associated with the variations of magnetization shown in the black thin lines in particular for the low-field and low-temperature region in the pulse-field results are due to the low resistivity of the sample e–h The estimated positive MR contributions due to the emergent-field fluctuations which correspond to the color-shaded regions in a–d i–l Magnetic-field dependence of thermopower at low temperatures measured with a 25 T superconducting magnet (T = 2 We have unraveled an unusual magneto-Seebeck effect in MnGe which shows a large enhancement by applying external magnetic field Through examining its origin from every possible aspect by photoemission spectroscopy we propose that the anomalous enhancement is rooted in strong energy dependence of transport lifetime τ which may arise from the H-dependent dynamics of emergent magnetic field high-field measurements on MR and S verifies the presence of surviving magnetic fluctuations to scatter the conduction electrons even in the FM phase The most important integrant for the observed thermoelectric phenomena in MnGe should be the dense lattice of magnetic singularities like spin hedgehogs and anti-hedgehogs where their large emergent fields and fluctuations critically affect the motion of electrons that is the efficient heat-electricity conversion of topological origin may lead to new guiding principles of achieving high thermoelectric performance in topological magnets A single crystal of MnSi was grown by the Czochralski method in tetra-arc furnace under an argon atmosphere Powder X-ray diffraction pattern of the pulverized single crystal indicated that the sample was of single phase Magneto-resistivity and specific heat capacity were measured by using AC-transport option (AC excitation current of 23 Hz and 20 mA) and heat capacity option with Physical Property Measurement System (PPMS) Magnetic field was applied parallel to electrical current for magneto-resistivity measurement High-field measurements of magnetization and longitudinal magneto-resistivity were performed utilizing nondestructive pulsed magnets installed at International MegaGauss Science Laboratory of Institute for Solid State Physics (ISSP) Magnetization was measured by the conventional induction method Resistivity was measured by the conventional four probe method with voltage pre-amplifiers using a numerical lock-in technique with an excitation current of 25 kHz and 20 mA Photoemission spectroscopy on MnGe was performed with a VG-Scienta R4000WAL electron analyzer and a helium discharge lamp with the photon energy of 21.2 eV at the University of Tokyo The Fermi energy was determined from the photoemission spectrum of a gold film evaporated on the substrate within an accuracy of better than ± 0.3 meV The MnGe sample was fractured at 11 K in an ultrahigh vacuum better than 1 × 10−10 Torr We confirmed the reproducibility of the temperature-dependent photoemission spectrum by the temperature-cycled measurements The application of Onsager’s reciprocal relations to thermoelectric Anomalously large thermoelectric cooling figure of merit in the Kondo systems CePd3 and Celn3 Thermoelectric transport in perfectly conducting channels in quantum spin Hall systems Enhanced thermoelectric performance and anomalous Seebeck effects in topological insulators Quantal phase factors accompanying adiabatic changes Large thermoelectric power in NaCo2O4 single crystals The chiral anomaly and thermopower of Weyl fermions in the half-Heusler GdPtBi Topological Nernst effect in a three-dimensional skyrmion-lattice phase Real-space observation of short-period cubic lattice of skyrmions in MnGe Real-space observation of a two-dimensional skyrmion crystal Longitudinal and transverse thermoelectric transport in MnSi Electrons and Phonons: The Theory of Transport Phenomena in Solids Ch Large enhancement of emergent magnetic fields in MnSi with impurities and pressure Critical features of colossal magnetoresistive manganites First performance test of a 25 T cryogen-free superconducting magnet Quantum ESPRESSO: a modular and open-source software project for quantum simulations of materials Soft self-consistent pseudopotentials in a generalized eigenvalue formalism BoltzWann: a code for the evaluation of thermoelectric and electronic transport properties with a maximally localized Wannier functions basis wannier90: A tool for obtaining maximally localised Wannier functions Download references Mitsuishi for their cooperation on photoemission spectroscopy This work was supported by JSPS KAKENHI (Grants Nos 24224009 and 15H05456) and JST CREST (Grant No A part of study was performed at International MegaGauss Science Laboratory of Institute for Solid State Physics and at High Field Laboratory for Superconducting Materials Department of Applied Physics and Quantum Phase Electronics Center (QPEC) synthesized polycrystalline samples of MnGe and performed transport and specific heat capacity measurements conducted photoemission spectroscopy under the supervision of K.I performed calculations of band structure and Seebeck coefficient performed high-field measurements of magnetization and magneto-resistivity using pulsed magnet at ISSP conducted high-field measurements of Seebeck effect using 25 T CSM at IMR under the supervision of A.T analyzed the data and wrote the draft with support from T.S. The authors declare no competing financial interests Download citation DOI: https://doi.org/10.1038/s41467-018-02857-1 AD Leave a rating/comment#PaintingBack to ArticlesSHARE Which language would you like to use this site in In response to the news that three people were hanged in Japan today Death Penalty Advisor at Amnesty International “The recent appointment of Prime Minister Fumio Kishida was a chance for progress on human rights in Japan But today’s abhorrent resumption of executions is a damning indictment of this government’s lack of respect for the right to life this feels like a missed opportunity for Japan to take long overdue steps to abolish the cruel practice of the death penalty “More than 100 countries worldwide have completely abolished the death penalty in law and two-thirds in total have abandoned it in law or practice “It is dismaying that Japan bucks this trend by continuing to use this cruel and inhuman punishment It is high time to establish a moratorium on all executions as a first important step.” Three death row inmates – Yasutaka Fujishiro Mitsunori Onogawa and Tomoaki Takanezawa – were hanged on Tuesday Japan is one of a handful of countries that has persistently executed in recent years Amnesty International recorded 483 executions in 18 countries excluding China in 2020 the lowest number of executions recorded in at least a decade Executions in Japan are shrouded in secrecy with prisoners typically given only a few hours’ notice and some given no warning at all before their death sentences are carried out Their families are usually notified about the execution only after it has taken place Amnesty International has repeatedly called on Japan to establish an immediate official moratorium on all executions as a first step towards total abolition These are the first executions under Prime Minister Fumio Kishida Yasutaka Fujishiro killed seven of his relatives in 2004 while Mitsunori Onogawa and Tomoaki Takanezawa were convicted of two murders in 2003 but the court ruled that he could be held criminally responsible for his actions Onogawa had filed a second request for a retrial a decision about which was still pending when he was executed Takanezawa had previously withdrawn an appeal to the high court filed by his lawyer His lawyer requested the court to cancel the withdrawal Japan’s most recent execution prior to today took place in December 2019 when a Chinese man convicted of four murders was hanged Amnesty International opposes the death penalty in all cases without exception regardless of the nature or circumstances of the crime innocence or other characteristics of the individual or the method used by the state to carry out the execution Together we can fight for human rights everywhere Your donation can transform the lives of millions If you are talented and passionate about human rights then Amnesty International wants to hear from you Your browser does not support JavaScript, or it is disabled．Please check the site policy for more information National Report Japan carried out its first executions in two years on Dec suggesting the new Kishida administration will continue to implement a hardline stance against capital crimes occurred when Masako Mori was justice minister The latest hangings bring the number of convicts on death row to 107 who was sentenced to death for killing seven people including his relatives and neighbors in Hyogo Prefecture in 2004 were put to death separately at the Tokyo Detention House They were handed the death sentence for killing two people and stealing cash from one of the victims as well as an employer of another man they murdered in Gunma Prefecture in 2003 targeting relatives and neighbors in Kakogawa He apparently bore a grudge against the victims believing they looked down on and made light of him He initially fatally stabbed three relatives aged between 46 and 80 and seriously injured a woman Fujishiro continued his rampage by fatally stabbing four members of a family aged between 26 and 64 who also lived nearby His first trial at a district court ended with a death sentence arguing that he had a diminished mental capacity The Supreme Court dismissed the appeal in 2015 Takanezawa and Onogawa were sentenced to death in connection with two murder cases The first concerned the death by strangulation with a rope of a 47-year-old pachinko parlor employee inside a car parked on a mountain in Miyagi The court heard that the pair later broke into the pachinko parlor using a key taken from the man they had just killed and stole 3 million yen ($26,000) kept on the premises They abandoned the man’s body in a river in Gyoda in neighboring Saitama Prefecture They continued their crime spree by targeting another pachinko parlor employee The 25-year-old man was also strangled to death with a rope inside a parked car The pair stole 119,000 yen he had on him in cash They tried to enter the pachinko parlor by using the key they had taken from him They dumped his body in the same river in Gyoda They both pleaded guilty to charges of murder and robbery in their first trial at a district court and were sentenced to death but Takanezawa’s death sentence was confirmed after he withdrew the appeal in 2005 Onogawa argued that he only followed Takanezawa’s instructions because he was scared of him but the Supreme Court dismissed his appeal in 2009 during which time Yoko Kamikawa served as justice minister for a third time Kamikawa ordered the executions of 13 former members of the Aum Shinrikyo doomsday cult during her second stint in office in 2018 death sentences were confirmed for four people this year both first-graders at a junior high school in Neyagawa Another killed three men and tried to murder a fourth by getting them to swallow capsules containing a cyanide compound died of illness before their sentences could be carried out The average age of death row inmates now stands at 59 They have been detained for just over 13 years on average Inmates sue state over executions imposed on day of notification Zama serial killer drops appeal; sentence to be finalized Serial killer’s death sentence stands; deadline for appeal over Ex-death row inmate’s acquittal in 1966 case a step closer Death sentence finalized for ‘Black Widow’ killer of 3 men Prosecutors seek death penalty for man over murder of 6 in Ibaraki Information on the latest cherry blossom conditions Please right click to use your browser’s translation function.) A series based on diplomatic documents declassified by Japan’s Foreign Ministry Here is a collection of first-hand accounts by “hibakusha” atomic bomb survivors chefs and others involved in the field of food introduce their special recipes intertwined with their paths in life A series about Japanese-Americans and their memories of World War II In-house News and Messages No reproduction or republication without written permission said the stories stumbled upon along the way are often his most treasured For his new documentary about George Stout a fellow Iowan who helped recover and preserve art endangered by World War II Kelley found himself in an unlikely apartment in Detroit Stout was one of the now famous Monuments Men Kelley and producer Marie Wilkes spoke with historians This path led them to the apartment of 95-year-old Motoko Fujishiro Huthwaite "Her eyes lit up as we spoke," Kelley said Displayed on a table in Fujishiro's room was a Congressional Gold Medal with a Dwight Eisenhower quote in relief: "It is our privilege to pass on to the coming centuries treasures of past ages." Kelley came to Fujishiro's apartment because she worked for a time for Stout and the Monument Men Fujishiro's story began well before the end of WWII. In her interview Kelley heard firsthand what a fearful country was capable of The damage done by the bombing of Pearl Harbor extended well past the lagoon that day The mainland did not have to be anywhere near Oahu to have felt the attack to fear the attacker. It was this fear that led the United States to intern its own citizens a first generation Japanese immigrant to the U.S., ran a dental practice there in the city Fujishiro told Kelley that life became more difficult after the war broke out as concerns about spying and national security were used to rationalize racist suspicion against Japanese nationals Both of her parents were Japanese citizens her family decided to take refuge in Japan was arrested by the FBI and sent to an internment camp.  Fujishiro survived the terror of Allied bombings in Tokyo And when Japan surrendered and the United States occupation began Fujishiro got a job as a clerk working for the Civil Information and Education Section under the Supreme Commander for the Allied Powers. She was part of the organizational effort to prevent looters and American soldiers from stealing art. It was for this work that she received the Congressional Gold Medal in 2015 "I was watching her through the lens of my camera and she was telling her story," Kelley said "And I was thinking these are the kinds of things when you make documentaries that make them more powerful." Even if it seems circuitous, the context Fujishiro offers to Stout's story informs the work that his protagonist George Stout was doing to protect art in Japan "I didn't think we would go into the (United States') concentration camps but when I first started researching George Stout I learned about Motoko and it's a part of this story," Kelley said "There was a tremendous amount of fear after the surrender about what would happen to the art in Japan." What began as an interesting wrinkle helped fill out the story and echoed for Kelley a phenomenon he is seeing today.  "Today there is a lot of fear," Kelley said. "What I love about history is the parallels you can see with the country today Kelley's documentary Stout Hearted: George Stout and the Guardians of Art will have its Iowa City premiere at 7 p.m The screening will begin with a Q&A with Kevin Kelley a former congressman; Giselle Simón a conservator with the University of Iowa Libraries; and members of the Stout family Tickets can be pre-purchased at icfilmscene.org or at the box office SUPPORT LOCAL JOURNALISM: Subscribe to the Iowa City Press-Citizen. Zachary Oren Smith writes about government growth and development for the Press-Citizen Reach him at zsmith@press-citizen.com or 319-339-7354 FILMSCENE HAPPENINGS:Why FilmScene is showing nothing but films made by women in March The July issue of Square Enix's Shonen Gangan magazine revealed on June 12 that Takeshi Fujishiro will launch a new manga titled RiriRe Customize - Modeler mo Isekai de Renkinjutsushi!? (Even a Modeler is an Alchemist in a Parallel World?!) on Square Enix's Gangan Online website on July 1 Fujishiro recently ended the Cafe Tanteibu (Cafe Detective Club) manga last November Square Enix published the manga's 12th and final volume on April 12 The manga launched on Square Enix's Gangan Online website in 2009 Fujishiro also drew the Nagasarete Airantou manga. The 2007 anime adaptation streamed briefly with subtitles on the now-defunct Daisuki streaming service Japanese artist Fujishiro Seiji has unveiled a special new work inspired by the ongoing suffering in Ukraine His portrait depicts the country's President Volodymyr Zelenskyy holding sunflowers Sunflowers turning toward the sun — they are a symbol of light and peace the 98-year-old told NHK in an interview in Tokyo Fujishiro says the images from Russia's invasion of Ukraine have triggered his own difficult memories from World War Two He decided to pick up his brushes to capture his thoughts and emotions Fujishiro drew upon a 1970 Italian movie classic "I Girasoli" that tells the story of people torn apart by war "I hope the people of Ukraine can live well "I want them to feel the joy of living — not the pain of military invasion Fujishiro is a pioneer of the genre known as shadow painting His works are filled with colors and often depict a dream world Some of the themes he explores include light The artist lived through World War Two and served in the now-defunct Imperial Navy but was ordered to produce Molotov cocktails with other young servicemen were sent to their deaths on kamikaze suicide missions – and he still struggles with their loss "The same tragedy is being repeated now," says Fujishiro He says the images from Ukraine summon his memories of Tokyo "People's lives are the most important thing I am so angry to see the Russians invade." Fujishiro has created works that reflect on World War Two They feature the Atomic Bomb Dome in Hiroshima he never used it as subject matter before 2005 He says he only started doing so to prevent memories of the war from fading "It's wrong to develop weapons that kill people and to possess a lot of them I want people to think more about peace as the whole world is connected." artist Uso Fujishiro’s one-man exhibit titled “Charatronica” will be at Earth+ Gallery in Koto The curator of the exhibit will be Noriyuki Tsuji (Geijutsu Keisu) Fujishiro is an energetically active artist who is the leader of the human-powered SNS group of Internet-based artists calling itself “Post Poppers” and was previously the representative of the art group “Chaos Lounge.” In addition to creating work based constantly on characters from pop culture anime because of the collective power of artists and creators from Twitter and other SNS sites that have art message boards is being praised from all sides for being a model case of an Internet-based artist exhibiting at a real gallery Beginning with his representative illustration “Hametsu Lounge” (2010) who had many opportunities to create group work will announce a new illustration series that shares its name with the exhibit Due to the work being a symbol for Fujishiro’s practice of continuing to create art based on the concept of “characters,” it can be said that this is a must-see Making full use of the space of the gallery the exhibit will be organized into two sections In addition to the aforementioned new series other works to be on display include “Day” and “Night,” the basis to which came about after Fujishiro visited the area affected by the Tohoku earthquake in March 2011 a lounge space that will seemingly function like a cafe area will include Fujishiro’s past work talk events will be held at the exhibit on July 20 and 27 who wrote the criticism “Ghost no Jouken,” will talk on July 20 known for his research on the cultural representation theory Event InformationGeijutsu Keisu Presents Uso Fujishiro’s One-Man Exhibit “Charactronica”Dates: July 13 2013 (Sun) (Closed on Mondays)Times: 11:00 a.m - 7:00 p.m.Planning: Noriyuki Tsuji (Geijutsu Keisu)Entrance Fee: Free (There will be a participation fee for the talk events)Location: Earth+ GalleryAddress: 3-18-17 1F Kiba Event ItineraryOpening Reception: July 13 (Participation is free)Talk Events: 2,000 yen participation cost (Includes one drink)- Yuichi Murakami (Critic): July 20 - 8:00 p.m.- Yoshiharu Ishioka (Researcher/Critic): July 27 Information on related events going on during the exhibit may be changed or updated on Geijutsu Keisu’s website or Earth+ Gallery’s website Source:http://kai-you.net/article/597 (Japanese) The timetable and area map have been announced for Japan’s largest idol festival Tokyo Idol Festival 2013 to be held on July 27 and 28 at Zepp Tokyo and other locations within Odaiba Calbee will release the “Baki the Grappler” collaborative snack “Baki Potato Chips Strongest Meat on Earth Life&Culture Japanese shadow painting master delivers hope Fairy tale illustrations of shadow and light are on exhibition at “Fantasy of Light and Shadow” being held at the Hangaram Arts Center Museum in southern Seoul offering visitors an extraordinary experience “Kage-e,” which literally means shadow painting in Japanese was pioneered by Japanese artist Seiji Fujishiro The exhibition is the Kage-e master’s largest solo exhibition in Seoul introducing some 160 artworks that he created over the past 70 years started out as an oil painter but began exploring the new genre during World War II as it became difficult to secure brushes and painting oil in the chaos of the war colored cellophane cutouts to create a picture The paintings come to life when light is reflected behind the paintings we can create something like the purest beauty,” he once said “The artist recalled he would not have thought of Kage-e if it not for the war,” said Kang Hye-sook director of Kart Communication who is organizing the exhibition “Fujishiro experienced the crucial times in Japanese history and the 1960s and 1970s when Japanese pop culture rapidly changed He is now 98 years old and it is a great inspiration to many people that he still actively continues his career as an artist.” While Fujishiro witnessed the tragedy of war he strived to deliver hope and bright messages through his art Many of his Kage-e paintings feature vivid colors and fantastical worlds with child-like imagination The exhibition includes “The Legacy of a Sad and Beautiful Peace” (2005) an artwork which shows the Hiroshima Atomic Bomb Dome The dome was destroyed in the nuclear bombing in 1945 in which more than 200,000 people were killed In a catalogue accompanying the exhibition Fujishiro recalled how he felt seeing what was left of the structure He stayed there for seven days to sketch the remains of the dome The rubble was turned into a beautiful Kage-e painting with water lilies surrounding the building as paper cranes fly above it The artist specifically created “Sleeping Forest” for the Seoul show “I am putting all my heart for the exhibition in Seoul I want to learn more about South Korea and want to get closer to South Korea,” he said in the catalogue the Fujishiro Seiji Museum in Tochigi prefecture is dedicated solely to the artist’s works housing some 140 Kage-e paintings and 200 of his drawings A shadow theater and brick chapel with stained glass windows designed by the artist are also on site Lee Jae-myung leads in hypothetical three-way race with Han Duck-soo the presidential candidate of South Korea’s main liberal Democratic Party holds a lead in a potential three-way race for the country’s next election 15% of Korean wage workers have flexible work schedules: data Koryo saram are 'living bridges' of Kazakh-Korean ties 'Smile at me': Cancer-fighting senior influencer inspires with strength Netflix's 'The Devil’s Plan' returns with star-packed season 2 Seoul to host inaugural 'MyK Festa' in June Yoo Seung-ho returns to stage as Brutus in reimagining of 'Julius Caesar' Interview: A legend contemplates usefulness Respect wanes: Teaching no longer highly coveted job in S South Koreans get creative with Parents’ Day gifts Korean mothers are opting for C-sections at record rates Share on FacebookShare on X (formerly Twitter)Share on PinterestShare on LinkedInOne man has been arrested for his involvement in an armed robbery in late April of this year was arrested on May 8 and hit with federal charges of using force to interfere with interstate commerce That decision was reached as the firearms were "made outside the state of Alaska," prosecutors said related to an APD stolen vehicle investigation Fujishiro was in possession of one of the stolen Cash America weapons and later charged in state court records with armed robbery The federal document did not identify Fujishiro as being involved in the Cash America robbery but court records show he was booked on May 9 charged with stealing firearms as well as the armed robbery charges consistent with what happened at Cash America The case stems from that robbery of the Cash America pawn store on Fireweed at around 7:40 p.m three masked and armed men stormed the store which have not yet been filed but are expected the federal charge of affecting commerce will potentially mean more jail time for Paleka and Explosives Bureau (ATF) got involved because the robbery included the theft of eight guns; seven were hand guns Officers made the arrest earlier this week on May 8 Not only was Paleka captured on video camera but in a report filed by a special agent with ATF authorities say Paleka also left blood at the scene the camera captured Paleka smashing that case as well as pointing his gun "directly at the employees and several customers Paleka was arrested and later admitted to the crime stating that he knew he would be caught because his DNA was at the scene of the crime The other two people with Paleka have not yet been identified Paleka confessed that the robbery was his idea and that he went to the store two times before the robbery to "scope it out." However he stopped short at saying who helped him commit the crime "Paleka then enlisted the other two suspects to help him but would not identify who his co-conspirators were," the ATF agent wrote Paleka is currently listed in the online court database for state charges The film adaptation of the novel “She’ll Be Here in April” and its release on Friday The film is a romantic novel published in 2016 by Genki Kawamura who produced a number of films including “Suzume no Dokkiri” and last year became the first Japanese to win the Best Director Award at the 70th San Sebastian International Film Festival for “Hyakka” The main character Shun Fujishiro is played by Takeru Sato Fujishiro’s fiancée Yayoi Sakamoto by Masami Nagasawa who has directed music videos for Genji Yonezu’s “Lemon” and Aimiyoon’s “Marigold,” is directing his first feature film and Takeshi Kobayashi is in charge of music and Uyuni to recreate the magnificent world of the original story When I read the original work 7 years ago I sympathized with Fujishiro who was searching for the true meaning of love without knowing what love is what he gained and lost during his time with his girlfriend could get as close as possible to the true nature of love Nagasawa approached her work with the utmost sincerity who has a tenderness and fragility that makes her put others before herself and yet she lives her life harder and more carefully than anyone else but together we created the character of Fujishiro who learns the joy of falling in love and being united with someone in earnest was very sincere in his pursuit of the play while worrying together with the actors and we had a very fulfilling time during the filming I hope you will come to the theater to find out the answers for yourselves I am sure that there were some anxieties and distortions there but I also think that she was feeling bold enough to take a new step onto a new path but I felt at ease filming him because he plays the role and the story calmly I enjoyed working with him because I developed a sense of trust in him day by day Director Yamada is a director who is unfazed by anything and has a strong sense of what he is concerned about and I was able to trust his sense and sensibility I had the sense that the story was gradually developed on the set This is a film for people who are struggling with love I think it will be a film that makes people want to reconsider what it means to love someone The release of the film is still some time away but I would be happy if you could look forward to it I feel very honored to be able to act in this original work with my seniors whom I respect very much What kind of presence can I be as Haru in this work I kept thinking about it every day during the filming period and Uyuni on my first overseas job in my life and all of those countries showed me how incredibly beautiful they are I felt like I was playing spring with the world on my side and I spent every day frantically burning it into my mind I look forward to the day when I can bring such an experience to you all It must be seven years ago that Takeru Sato gave me an enthusiastic feedback when he read “April comes I have worked with him in various capacities and he told me that it was his favorite novel that I had written I still recall our deep discussion about a sentence I wrote about the question of “how not to lose love” in the novel but I feel that the issues of love and romance are still as complicated as ever The filmmaking process proceeded with careful discussions with Takeru Sato with the aim of making a romantic film for the current era I felt that Masami Nagasawa’s sincere approach to her work often overlaps with the role of Yayoi who gives light to people who are supposed to be working hard and earnestly but somehow find it hard to live Nanana Mori gave a wonderful performance as Haru who is like a “memory of my first love Her journey around the world took my breath away more than once but I now believe that it was necessary for this cast and crew to come together I would like to thank Takeru Sato for accompanying me in the making of this film from beginning to end and truly made this cast a dream come true The time I spent with these three sincere and supremely expressive people was exciting and irreplaceable Takeru Sato participated from the script meeting stage and we worked together to create a portrait of the characters through repeated discussions which is not a common practice in Japanese films whose role was newly adapted for the film version was a perfect embodiment of the “new elements beyond the original novel,” and she sincerely portrayed a character with a lovely and pure humanity that could never have been created by anyone other than Ms whom I had worked with in a previous short film and trusted immensely showed an impressive transparency and presence surpassing the script by far and leaving much to her talent on the set The contrast she brought to the film was shocking I am very proud to have had the opportunity to work with the three of you in this new expression She’s Here” (Japanese text only) In theaters nationwide at Toho on March 29 2024 Director : Tomokazu Yamada Screenplay : Yuichiro Kido Genki Kawamura Music : Takeshi Kobayashi Cast : Takeru Sato ©2024 “She’ll Be Here in April” Production Committee NiEW Best Music is a playlist featuring artists leading the music scene and offering alternative styles in our rapidly evolving society the NiEW editorial team proudly curates outstanding music that transcends size The 107th chapter of Takeshi Fujishiro's Cafe Tanteibu (Cafe Detective Club) manga announced on Thursday that the manga will end with the next chapter The manga launched on Square Enix's Gangan Online website in 2009 and Square Enix shipped the 11th compiled volume on May 22 The manga takes place at a school that is famous for accepting any and all student clubs The incoming freshmen are excited to start their own club but the staff put a limit on the new clubs that can be created Five girls want to create a detective club and retro gaming club end up teaming up to create the Cafe Detective Club Source: Cafe Tanteibu chapter 107 Boston’s only completely owned and operated full-service Japanese restaurant has recently undergone massive renovations and updated their menus Owners Lena Kikuchi and Yujin Kawakami-Hess announced this week that they’ll implement an official re-launch later this month The restaurant now features a remodeled outdoor patio overlooking Newbury Street and a casual-chic downstairs bar Executive Chef Kaoru “Fuji” Fujishiro has revamped the menu with plenty of new non-sushi dishes like his gratin a Japanese mac and cheese made with lobster Another addition is the “Doria,” a rice casserole served with cheese and a choice of proteins Fujishiro also consulted with with several chefs in Japan and developed Itadaki’s new ramen recipe The tonkotsu (pork-based) broth is the featured soup but the ramen will also available in shoyu or miso styles Itadaki has made tweaks to most of their small plates and their full-range of sushi pizzas—fried rice cakes topped with sashimi and other toppings Newbury Street’s newest patio will be open seven days a week for lunch and dinner 269 Newbury St., Boston; 617-267-0840 or  itadakiboston.com Here’s Where to Find the Top Doughnuts in Boston Where to Eat in Greater Boston in April 2025 Where to Eat in Greater Boston in May 2025 photographer Meisa Fujishiro has transcended the boundaries of gravure and she continues to present her unique worldview even now Meisa Fujishiro's early photo book "90 NIGHTS" is now on sale This book is a compilation of the energy that swirled with the rise of DJ music captured on film over a period of four to five years with a Konica Big Mini in hand at a time when new nightlife venues such as the now-defunct GOLD and Daisan Soko in Shibaura and CAVE in Shibuya were opening one after another and a new movement was taking off in Tokyo The film is a collection of images of the club's residents captured over a period of four to five years with famous DJs and creators among those who appear in the book a photo exhibition is currently being held at "BOOKMARC" in Harajuku (until June 3) featuring works including those not yet published in the book Please take advantage of this unique opportunity to experience the world view of the time Text_Jun Nakada Today's print edition Home Delivery the term "zakka," which literally means "uncategorizable things," was usually used for mundane everyday tools and objects such as kettles it has evolved to mean miscellaneous goods of a much broader range and is often used by stores for toys housewares and even groceries.googletag.cmd.push(function() { googletag.display('div-gpt-ad-1499653692894-0'); }); Through the works of artists Shinya Aota and Phillipe Weisbecker and various objects selected by professionals of various fields — including stylist Miyoko Okao art director Naomi Hirabayashi and Morioka Shoten bookstore owner Yoshiyuki Morioka — this exhibition explores the culture of zakka the variety of things it encompasses and the role it plays in our lives 21_21 Design Sight; 9-7-6 Akasaka, Minato-ku, Tokyo. Roppongi Stn. 10 a.m.-7 p.m. ¥1,100. Closed Tue. 03-3475-2121; www.2121designsight.jp/en In a time of both misinformation and too much information quality journalism is more crucial than ever.By subscribing Your subscription plan doesn't allow commenting. To learn more see our FAQ Sponsored contents planned and edited by JT Media Enterprise Division Please view the main text area of the page by skipping the main menu. The page may not be displayed properly if the JavaScript is deactivated on your browser