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The page may not be displayed properly if the JavaScript is deactivated on your browser Japanese version the ‘urban vessel’, by nano architects resides along the onga river in nogata city the plot sits amongst dozens of showa-era buildings evoking images of moored vessels sitting patiently in the harbor awaiting crews to bring them into the world nano rescues the neighborhood with the ‘urban vessel’ residence the home’s design carries three traditions spawning from the vernacular ‘nagaya’ style: the linear site from land division the ‘urban vessel’ fuses elements of the past the ‘urban vessel’ leads inhabitants out of the storm and to their destination designboom has received this project from our DIY submissions  feature, where we welcome our readers to submit their own work for publication. see more project submissions from our readers here AXOR presents three bathroom concepts that are not merely places of function but destinations in themselves — sanctuaries of style Look out for your first newsletter in your inbox soon We help you navigate a myriad of possibilities Sign up for our newsletter for the best of the city By entering your email address you agree to our Terms of Use and Privacy Policy and consent to receive emails from Time Out about news, events, offers and partner promotions. Tokyo Just three minutes’ walk from Nogata Station on the Seibu-Shinjuku line, this noodle shop is best known for its rich miso ramen (¥950). The basic broth is made with white miso, garlic and lard, then mixed with pork, chicken and vegetable stock and cooked into a soup. The result is a rich but not overbearing ramen, with a taste that clearly keeps Hanamichi’s customers coming back. If you’ve got a big appetite, you can add in the house mix of leek and bean sprouts at no extra cost – just make sure you can finish all the food in your bowl. Other options on the menu include miso dandan noodles, niboshi (dried sardines) spicy miso and abura soba (soupless ramen noodles). A ramen stamp card is also available if you’re thinking of becoming a regular. facebooktwitterpinterestinstagramAbout us This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page By entering your email address you agree to our Terms of Use and Privacy Policy and consent to receive emails from Time Out about news, events, offers and partner promotions. Let's face it: connecting Shinjuku with Saitama's Kawagoe, the Seibu Shinjuku line and its yellow carriages simply don't have the name recognition of the Chuo line, Tokyo's other main westbound railway. Compared to the Chuo's Koenji and Nakano known as some of the city's trendiest 'hoods Numabukuro and Araiyakushi-mae along the Seibu are quiet and unremarkable rarely attracting neither positive nor negative attention that state of affairs has allowed these spots to develop a quirky culture all of their own something like a suburban version of that found on the picturesque streets of Yanesen 'Aranumano' (for Araiyakushi, Numabukuro and Nogata and yes we made it up) is where you can soak at an age-old public bath after trying your hand at ping-pong with the locals exploring the backstreets of run-down shopping arcades or visiting a philosophically inspired park before descending into a world of old-school izakayas ethnic eateries and box-sized cafés Our three-part area guide series shows you where to eat stroll and drink in Aranumano: first up is Nogata the westernmost of the three stations and a worthy stop for gourmands on a budget Thanks for subscribing! Look out for your first newsletter in your inbox soon! in cooperation with ITOCHU Techno-Solutions Corporation will start a demonstration experiment of a reservation-based shared-ride cab service using AI (artificial intelligence) to solve social issues in the community.The two demonstration areas are Kamitono and Hata/Eimanji Both of these areas are considered transportation blank areas with few bus routes it is a bit inconvenient to go shopping or to the hospital We will start a service that allows people to transfer to existing local buses and community buses by using Mobility as a Service (MaaS) which is a service that brings together information on railroads and allows people to search and make reservations via an application or phone call Users are free to set their own pick-up and drop-off locations within the area AI determines the most efficient route for transporting multiple users and operates a shared-ride cab service the AEON MALL Nogata can be used as a starting point and destination in the Kamitono area the starting point and destination can be near the Domeki bus stop or near the Tonno post office to connect to existing bus routes in the midst of difficult business conditions for railroads and bus routes we aim to find a solution for a sustainable public transportation system in the future by coexisting with existing bus routes.How much will the system actually be used by local residents By determining through this demonstration experiment whether this will lead to the revitalization of public transportation it is likely that it will also lead to solutions for communities with similar issues interpoint (interword separation)implementation period 　May 9 2022  interpoint (interword separation)Operating Hours 　Weekdays 8:00 a.m to 5:00 p.m.interpoint (interword separation)Reservation acceptance time 　Smartphone application: 24 hours　Phone: 8:00 a.m weekdaysinterpoint (interword separation)fare　300 yen per adult (150 yen for elementary school students and younger and persons with disabilities)/person 　*Free of charge from May 9 For those wishing to publish articles/promotional support Those who wish to support open innovation Metrics details A Corrigendum to this article was published on 10 May 2017 This article has been updated With the aim of identifying sex determinants of fig we generated the first draft genome sequence of fig and conducted the subsequent analyses Linkage analysis with a high-density genetic map established by a restriction-site associated sequencing technique and genome-wide association study followed by whole-genome resequencing analysis identified two missense mutations in RESPONSIVE-TO-ANTAGONIST1 (RAN1) orthologue encoding copper-transporting ATPase completely associated with sex phenotypes of investigated figs This result suggests that RAN1 is a possible sex determinant candidate in the fig genome The genomic resources and genetic findings obtained in this study can contribute to general understanding of Ficus species and provide an insight into fig’s and plant’s sex determination system (A) Monoecious caprifig fruit and (B) female fig fruit red and green arrowheads indicate staminate flower short-style pistillate flower and long style pistillate flower caprifig short-style pistillate flowers (center) and fig long-style pistillate flower (right) dominant allele for gynoecious flowers short-style pistils; g recessive allele for gynoecious flowers short-style pistils; A dominant allele for presence of the androecium; a recessibe allele for suppression of the androecium Two genes G/g and A/a are considered to be closely linked Table was generated in reference to Storey (1975) quantitative trait locus (QTL) mapping and genome wide association study (GWAS) would be necessary and a straightforward strategy we thereby organized the first draft genome of fig and examined its overall sequence characteristics We subsequently conducted a restriction site-associated DNA sequencing (RAD-seq) analysis of an F1 population to construct the first-ever high-density linkage map on the basis of genome-wide single nucleotide polymorphisms (SNPs) Using the genomics resources for the genetic analyses we searched a series of molecular databases for staminate flower forming gene putatively involved in sex determination of fig individuals The result led to the discovery of a prime candidate gene RESPONSIVE-TO-ANTAGONIST1 (RAN1) The genomic resources and genetic findings obtained in this study not only contribute to general understanding and improvement of fig species but also provide an insight into plant’s sex determination system Since the draft genome sequence would cover large parts of gene-rich regions in the fig genome we concluded that the genome sequence data would be sufficient to discover genes for sex determination in fig Total 7498 SNP markers were mapped to the 13 linkage groups corresponding to 13 chromosomes Most of marker positons were conserved between Caprifig and Fig maps (A) pseudo chromosome comparison between fig and jujube (Ziziphus jujube) one to one relationship of pseudo-chromosomes was confirmed except for Fc01 Fig pseudo-chromosome which correspond to jujube Chr1 is divided into two pseudo-chromosomes Fc01a and Fc01b Fig chromosome number was determined by reference to correspondent jujube chromosome number (B) pseudo chromosome comparison between fig and peach (Prunus persica) (C) pseudo chromosome comparison between fig and Japanese apricot (Prunus mume) predicted functional impacts on genes in the draft genome sequence were categorized into four classes (A) MADF (Male DNA Associated Sequence) markers and seq000259_8998 marker positioned at the diagonal region of Fc01a chromosome Most statistically significant SNP markers were mapped to the scaffold seq000259 on the Fc01a chromosome seq000259_8998 marker genotypes completely matched the sex phenotypes of 119 test materials sex controlling and sex evolution model in fig Each of 2nd exon and 7th exon has one missense variations at 12,314th and 9,876th positon respectively The results suggest that RAN1 is a prime candidate for the sex determining A gene in fig We also successfully predicted a candidate gene for sex determination which indicate that the current draft genome assembly should be sufficient for subsequent post-genome studies in fig If either or both of RAN1 variations correspond with the former mutation RAN1 is responsible gene for creating females We are currently analysing the function of the RAN1 gene through transgenic experiments As for the dioecy sex determination, the OGI/MeGI gene system, has been reported in Diospyros lotus33 The model proposed to explain this system is that OGI encoding a small RNA and homologous to MeGI influences the function of MeGI in an oppressive manner through RNA silencing Although fig is functionally dioecious and thus different from D this model might be applicable to fig as well the presence of MeGI was confirmed in the draft fig genome (not linked to the linkage map) whereas no OGI sequence homologous to the MeGI sequence was observed in the present sequences of female or male individuals Fig may therefore be a suitable species for genomic applications such as GWAS and genomic selection Future applications of the fig genomic information uncovered in this study would contribute to an understanding of its useful and unique properties and to the improvement of its productivity and marketability Total genomic DNA that was extracted from leaves of the fig cultivar Horaishi using a DNeasy plant kit (Qiagen USA) was subjected to library construction Paired-end and mate-pair libraries with multiple insert sizes (500 to 5,000 bp) were constructed for Illumina sequencing according to the manufacturer’s instructions (Illumina Five rules were applied during processing of the raw data: (1) adapter removal (2) removal of leading low-quality (quality score < 3) or ‘N’ bases (3) removal of trailing low-quality or N bases (4) scanning of reads (using a 4-base-wide sliding window) and removal when the average quality per base dropped below 15 and (5) removal of reads less than 36 bases long 18.6 Gb of high-quality data were retained representing approximately 60× genome coverage To find genome-wide SNPs and indel polymorphisms were used for the whole-genome resequencing analysis we combined all data sets to generate a high confidence gene set We performed functional gene annotations by BLASTP InterProScan and KEGG KAAS alignments to nr Gene ontology and KEGG orthology terms were obtained from the corresponding InterProScan and KEGG entries Nucleotide sequences of the libraries were determined on a HiSeq (Illumina) system in paired-end The sequence data was processed and mapped onto the genome assembly SNPs called from the mapping alignments were used for linkage and association analysis Sex phenotype data were collected at the Fukuoka Agriculture and Forestry Research Center The default parameters of SnpEff were used to perform the variant effect analysis The complete RAN1 coding regions of two sexes (Caprifig6085 and Horaishi) were amplified from fruit totalRNA using PrimeScript™ II High Fidelity One Step RT-PCR Kit (Takara Bio FcRAN1-F1 (5′-ATGGCGGCGAGCGTCCGACACCT-3′) and FcRAN1-R1 (5′-TTATTTCTAGTATAGTGGTCAGC-3′) The amplified products were purified and directly sequenced using a Big-Dye Terminator v3.1 Cycle Sequencing kit on an ABI 3730xl platform (Applied Biosystems Accession codes: The fig genome data have been deposited in DDBJ/EMBL/GenBank under accession code BDEM01000001-BDEM01027995 (27995 entries) Sequence reads of transcriptome sequencing have been deposited in the NCBI sequence read archive under accession code DRA004650 Identification of RAN1 orthologue associated with sex determination through whole genome sequencing analysis in fig (Ficus carica L.) Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations A correction has been published and is appended to both the HTML and PDF versions of this paper Scientific Reports 7: Article number: 41124; published online: 25 January 2017; updated: 10 May 2017 This Article contains errors In the legend of Figure 1: ‘(D) Genetics of sex determination in F dominant allele for gynoecious flowers short-style pistils’ Should read: ‘(D) Genetics of sex determination in F horticulture and breeding In Horticultural Reviews Early domesticated fig in the Jordan Valley FAO., FAO STAT., http://faostat3.fao.org/home/E (2013) (Date of access: 09/09/2016) is regulated by light and associated with inflorescence differentiation in fig (Ficus carica L.) Sex Differences and Flowering Phenology in the Common Fig Two new nuclear isolation buffers for plant DNA flow cytometry: a test with 37 species De novo sequencing and comparative analysis of expressed sequence tags from gynodioecious fig (Ficus carica L.) fruits: caprifig and common fig The complex jujube genome provides insights into fruit tree biology The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity Multi-gene analysis provides a well-supported phylogeny of Rosales The Sequence Ontology: a tool for the unification of genome annotations A program for annotating and predicting the effects of single nucleotide polymorphisms SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3 Draft genome sequence of the mulberry tree Morus notabilis University of California Division of Agricultural Sciences 3 myr-old species of Cucurbitaceae with the largest Y/autosome divergence in flowering plants Stevens, P. F. Angiosperm Phylogeny Website Version 13, http://www.mobot.org/MOBOT/research/APweb/ (2001 onwards) (Date of access: 09/09/2016) Plant contributions to our understanding of sex chromosome evolution The M locus and ethylene control sex determination in andromonoecious cucumber plants The influence of ethylene perception on sex expression in melon (Cucumis melo L.) as assessed by expression of the mutant ethylene receptor under the control of constitutive and floral targeted promoters A cucurbit androecy gene reveals how unisexual flowers develop and dioecy emerges Flower development: sex lessons from cucumbers a Menkes/Wilson disease-related copper transporter is required for ethylene signaling in Arabidopsis Metal ions are involved in ethylene perception and signal transduction Empirical Studies: Evolution and Maintenance of Dimorphic Breeding Systems Gender and sexual dimorphism in flowering plants Berlin: Springer-Verlag 33–60 (1999) The mechanism of sex determination in dioecious flowering plants A model for the evolution of dioecy and gynodioecy Y-chromosome-encoded small RNA acts as a sex determinant in persimmons Efficient de novo assembly of highly heterozygous genomes from whole-genome shotgun short reads a database of eukaryotic repetitive elements De novo identification of repeat families in large genomes Smit, A. F. A., Hubley, R. & Green, P., RepeatMasker Open-4.0, http://www.repeatmasker.org Using native and syntenically mapped cDNA alignments to improve de novo gene finding Primer3 on the WWW for general users and for biologist programmers A second generation framework for the analysis of microsatellites in expressed sequence tags and the development of EST-SSR markers for a conifer Analytical workflow of double-digest restriction site-associated DNA sequencing based on empirical and in silico optimization in tomato JoinMap 4: software for the calculation of genetic linkage maps in experimental populations (Kyazma B.V. MapChart: software for the graphical presentation of linkage maps and QTLs Gapped BLAST and PSI-BLAST: a new generation of protein database search programs Circos: an information aesthetic for comparative genomics PLINK: a tool set for whole-genome association and population-based linkage analyses SNP discovery and linkage map construction in cultivated tomato TopHat: discovering splice junctions with RNA-Seq Download references This work was supported in part by a grant from the Ministry of Education Science and Technology/Japan Society for Promoting Science (KAKENHI grant no Fukuoka Agriculture and Forestry Research Center Buzen Branch Fukuoka Keichiku Agricultural Extension Center Fukuoka Agriculture and Forestry Research Center wrote the manuscript with contributions from K.M The authors declare no competing financial interests Download citation Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. 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Volume 12 - 2021 | https://doi.org/10.3389/fpls.2021.647599 This article is part of the Research TopicBreeding Innovations in Underutilized Temperate Fruit TreesView all 11 articles The common fig (Ficus carica L.) has a gynodioecious breeding system and its sex phenotype is an important trait for breeding because only female plant fruits are edible During breeding to select for female plants which is strongly associated with the sex phenotype In 12 F1 populations derived from 13 cross combinations the FcRAN1 genotype segregation ratio was 1:1 whereas the M119-226 × H238-107 hybridization resulted in an extremely male-biased segregation ratio (178:7 = male:female) This finding suggests that the segregation distortion was caused by some genetic factor(s) A whole-genome resequencing of breeding parents (paternal and maternal lines) identified 9,061 high-impact SNPs in the parents A genome-wide linkage analysis exploring the gene(s) responsible for the distortion revealed 194 high-impact SNPs specific to Caprifig6085 (i.e. seed parent ancestor) and 215 high-impact SNPs specific to H238-107 (i.e. A comparison between the annotated genes and the genes required for normal embryo or gametophyte development and function identified several candidate genes possibly responsible for the segregation distortion This is the first report describing segregation distortion in F we genotyped the FcRAN1 alleles of some breeding populations to identify sex phenotypes We observed that individuals with male type alleles are produced at a high rate only in a specific cross combination (M119-226 × H238-107) suggesting that the FcRAN1 locus is affected by segregation distortion Clarifying the cause of this distortion may lead to improved sex control and selection during fig breeding but it may also provide insights into sex ratio transitions and sex chromosome evolution in fig we examined the segregation distortion in fig breeding populations and performed preliminary analyses to explore the responsible genetic factors we investigated the distribution of SNPs specific to each pollen and seed parent generating segregation distortion in a whole-chromosome pseudomolecule and considered the biological and evolutionary background related to the occurrence of the segregation distortion F-test for the segregation distortion of the FcRAN1 genotype in the F1 population Genotyping results of FcRAN1 SNP by KASP assay Genotypic results for 10 progenies derived from 10 cross combination using 7 pollen parent lines The scatter plot with axes x and y represents allelic discrimination of FcRAN1 genotypes green dots represent the A/A homozygous (female fig type) A/G heterozygous (male caprifig type) respectively Optical microscope images of stained pollen grains (A) H238-107 (distorting line: left) and (B) Kibaru (CH13) (non-distorting line: right) Pedigree chart related to “M119-226.” Seed parent (Masui Dauphine) and paternal grand seed parent (Violette Solies) are marked with “ND” as parents that didn’t cause segregation distortion “M119-226” and the other genotypes are marked with “D” or “D?” as parents that caused or could cause segregation distortion Amino acid sequence alignment of FcLEA genes (LC639910) “Horaishi” is the variety of the reference genome “Caprifig6085” indicates a variety with segregation distortion and “Masui Dauphine” indicates a representative varietie of the group without segregation distortion The leucine residue marked with an asterisk (*) in FcLEA was mutated to proline to make a non-synonymous substitution Numbers in left side of sequence indicate the position of the beginning amino acid residue in each protein sequence The mapping of all GWLA SNPs to whole pseudomolecules indicated that high-impact SNPs were widely distributed throughout the genome in the seed and pollen parents, but there were no high-impact SNPs on chromosomes Fc03 and Fc06 of the seed parents (Figure 5) Schematic Diagram showing GWLA SNPs that mapped to the whole chromosome pseudomolecules (A) High impact SNP marker map in pollen parents and (B) high impact SNP marker map in seed parents Horizontal lines on each chromosome indicate the location of SNP markers The left side number indicate the distance from a telomere point of Fc01a chromosome pseudomolecule it was assumed that the Caprifig6085 and VC-119 genomes include a causative factor of segregation distortion Caprifig6085 was used as a substitute for M119-226 Since it is assumed that there are a wide variety of factors that can cause segregation distortion, the number of candidate genes involved is considered to be large corresponding to be the number of factors. For example, in Arabidopsis thaliana, 510 EMBRYO-DEFECTIVE (EMB) genes (Meinke, 2020) and 129 genes associated with female gametophyte deficiency (Pagnussat et al., 2005) have been identified all of which may contribute to segregation distortion 98 genes were detected as specific to M119-226 we identified several genes similar to the above-mentioned A thaliana genes related to female gametophyte deficiency The fertilization of the A. thaliana mutant line UNE15, which has a mutation in a gene encoding a LEA protein, is reportedly abnormal because of defective pollen tube guidance (Pagnussat et al., 2005) the female gametophytes of the seed parent M119-226 may be infertile because of a mutation similar to that in UNE15 other candidate genes may be responsible for segregation distortion including genes encoding proteins with unknown functions Y chromosome drive model for the role of distorter in Segregation Distortion (SD) is expressed during male meiosis to cut a target sequence on the X-chromosome The shredding of the X-chromosome favors the unaffected Y-carrying gametophyte and results in the production of a male-biased progeny The FcRAN1 KASP marker analysis indicated that some plants in the C100 population had the G/A genotype There are two possible explanations for this observation the effect of the distorter may have been incomplete chromosomal recombination) may have occurred in which the distorter gene was not activated Future studies should further characterize the genetic background of the distortion via crossing experiments involving multiple seed parents because the candidate genes discussed herein were selected on the basis of bioinformatics data their functions will need to be validated through genetic transformation or genome editing The high-impact SNPs in the pollen parents were within 5 Mbp from the end of Fc01a (Figure 5) suggestive of a relationship between the sex-linked region and the segregation distortion FcRAN1 or a nearby gene) basically affects the population sex ratio the distorter gene and FcRAN1 are likely located close together and behave similarly The biological or evolutionary significance of the segregation distortion observed in figs is unclear. However, the occurrence of segregation distortion can change the genetic composition of the species population and may maintain or increase the genetic diversity of the population. In fact, segregation distortion reportedly leads to stable large-scale genomic differences between males and females in hybrid ants (Kulmuni et al., 2010) Because the FcRAN1 genotype is strongly correlated with the sex phenotype, the genotypic distortion could influence the evolution of sexual reproduction and sex chromosomes in various ways (Kozielska et al., 2010). Fig X and Y chromosomes are homomorphic, with no significant differences in their molecular structure (Storey, 1975) indicating that fig sex chromosomes are like autosomal chromosomes and are not well differentiated clarifying the segregation distortion of the FcRAN1 genotype may help elucidate the differentiation of fig sex and autosomal chromosomes Seven seed parents (M119-226, V180-13, Masui Dauphine, H180-2, HIG5, Toyomitsuhime, and Burjassotte Greece) and six pollen parents (H238-107, M238-1, VD238-83, VS238-53, NG60, and CH13) were used for hybridizations, which were completed between 1989 and 2018 according to Storey (1975) The obtained seeds were sown in a seedbed and the resulting young seedlings were transplanted to 9-cm pots containing dedicated soil Leaf samples were collected from the seedlings for KASP and CAPS marker analyses as described below The SNP genotyping data generated from the KASP marker analysis were visualized using the SNPviewer software (LGC The pollen of each paternal line was immersed undisturbed in a 600 μl 1% (w/v) iodine solution for 5 min after which 50 μl of the mixture was added to a microscope slide Pollen grains stained orange or brown were considered to be viable and were counted The SNPs with an F_A (frequency of this allele in cases) of 1.0 or 0.5 and an F_U (frequency of this allele in controls) of 0.0 were selected as candidate SNPs responsible for the difference between distorting and non-distorting lines The FcLEA gene sequence has been deposited in DDBJ/EMBL/GenBank under accession number LC639910 Genome sequencing reads of caprifig lines have been deposited in the NCBI Sequence Read Archive under accession numbers DRA011350 and DRA012209 The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material All authors contributed to the article and approved the submitted version Science and Technology/Japan Society for Promoting Science (KAKENHI grant 19H02952 and 16H04878) The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2021.647599/full#supplementary-material Supplementary Table 1 | “Type” refers to the genetic segregation type: 1| 0 means that the target sample is homozygous for the reference sequence and the other samples are homozygous for the other sequence whereas 0.5| 0 means the target sample is heterozygous for the reference sequence and the other samples are homozygous for the other sequence Supplementary Table 2 | “Type” refers to the genetic segregation type: 1| 0 means that the target sample is homozygous for the reference sequence and the other samples are homozygous for the other sequence Google Scholar Uncovering the post-embryonic functions of gametophytic- and embryonic-lethal genes Google Scholar Dobrovolskaia-Zavadskaia Google Scholar and breeding,” in Horticultural Reviews Google Scholar Cloning and chromosomal mapping of a human immunodeficiency virus 1 “TATA” element modulatory factor A new sex-ratio abnormality in DROSOPHILA OBSCURA CrossRef Full Text | Google Scholar Genetic distortion of sex ratio in a mosquito CrossRef Full Text | Google Scholar Segregation distortion and the evolution of sex-determining mechanisms Segregation distortion causes large-scale differences between male and female genomes in hybrid ants The Arabidopsis TFIID factor AtTAF6 controls pollen tube growth PubMed Abstract | CrossRef Full Text | Google Scholar Genetic linkage map of Coffea canephora: effect of segregation distortion and analysis of recombination rate in male and female meioses Male sterility in natural populations of hermaphrodite plants the equilibrium between females and hermaphrodites to be expected with different types of inheritance CrossRef Full Text | Google Scholar The sequence alignment/map format and SAMtools The origin and behavior of mutable loci in maize PubMed Abstract | CrossRef Full Text | Google Scholar Genome-wide identification of EMBRYO-DEFECTIVE (EMB) genes required for growth and development in Arabidopsis Google Scholar Transient RNA-DNA hybrids are required for efficient double-strand break repair Google Scholar Parasitic nature of extra fragment chromosomes Google Scholar Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis Sex chromosome meiotic drive in stalk-eyed flies CrossRef Full Text | Google Scholar Fast identification and removal of sequence contamination from genomic and metagenomic datasets The Ficus erecta genome aids Ceratocystis canker resistance breeding in common fig (F A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae “Figs,” in Advances in Fruit Breeding Google Scholar CrossRef Full Text | Google Scholar Geographical distribution and cytology of “Sex Ratio” in Drosophila pseudoobscura and related species CrossRef Full Text | Google Scholar Sex ratio distortion caused by meiotic drive in a mosquito CrossRef Full Text | Google Scholar Common features of segregation distortion in plants and animals Google Scholar Preliminary characterization of “sex ratio” and rediscovery and reinterpretation of “male sex ratio” in Drosophila affinis CrossRef Full Text | Google Scholar Google Scholar Sex-ratio distortion caused by meiotic drive in mosquitoes Google Scholar Tashiro K and Nogata H (2021) Analysis of the Segregation Distortion of FcRAN1 Genotypes Based on Whole-Genome Resequencing of Fig (Ficus carica L.) Breeding Parents Copyright © 2021 Ikegami, Shirasawa, Yakushiji, Yabe, Sato, Hayashi, Tashiro and Nogata. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) distribution or reproduction in other forums is permitted provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited in accordance with accepted academic practice distribution or reproduction is permitted which does not comply with these terms *Correspondence: Hidetoshi Ikegami, aWtlZ2FtaUBmYXJjLnByZWYuZnVrdW9rYS5qcA== Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher 94% of researchers rate our articles as excellent or goodLearn more about the work of our research integrity team to safeguard the quality of each article we publish Today's print edition Home Delivery The article you have been looking for has expired and is no longer available on our system Japanese version Get quality reporting directly into your inbox Russia’s largest petrochemical company, Sibur, has long been associated with President Vladimir Putin. His old friend, oligarch Gennady Timchenko, acquired a significant stake in the company in 2011. And his former son-in-law made headlines when it was revealed that he picked up a 17-percent stake in Sibur that made him a billionaire leaked emails show that the company spent large sums on a villa linked to the president — with top-level employees not even understanding why The house is a stately mansion on the Gulf of Finland known as Villa Sellgren. In 2016, TV channel Dozhd reported that it had become a vacation destination for Putin In an independent investigation, opposition leader Alexei Navalny made the same claims, and pointed out that a banner near the villa’s entrance identified it as a recreation center for Sibur He suggested that the Sibur sign was a way of covering up Putin’s use of the villa A Sibur banner near the entrance to the villa highlighted by Alexei Navalny in a 2017 investigation (below) A leak of emails from the company obtained by OCCRP provides new evidence that his hypothesis was correct agreed in 2012 to pay over $1 million per year to rent the villa from its owner through a property management company called Nogata But the petrochemical giant was not actually allowed to use the facilities and some of its senior executives remained mystified by the arrangement “The director [of Nogata] told me she has a task even do corporate celebrations there,” wrote Sibur’s managing director Can we use it?” he asked the chairman of the company’s board has close links to Putin: It belongs to Nikolai Shamalov a friend of Putin and the father of Kirill Shamalov who married the president’s daughter in February 2013 a Nogata representative wrote to Sibur explaining that a new management company would take over running the property and that Sibur’s lease must be terminated within ten days To arrange a new lease with the new management company Sibur would need to speak with a man named Pavel Zaitsev Reporters found another connection between Sibur and Putin in the south of Russia Leaked emails show that a senior executive of the petrochemical giant enjoys the use of a heavily guarded Black Sea resort that appears to be associated with Putin’s nearby palace was owned by the town of Gelendzhik until 2014 It was then acquired by a private company called Investstroy which is owned by a woman named Tatiana Kuznetsova are closely associated with the famous “Putin’s Palace,” which is perched on a nearby cliff Kuznetsov is an employee of the Federal Protective Service and headed the military unit that helped develop the palace in the early stages of its construction was listed as the manager of the palace between 2014 and 2017 Putin’s Palace is held by an LLCInvest company and there are signs that Strela is also connected to the holding: Its director between 2014 and 2018 was Alexei Vasilyuk who headed two other LLCInvest companies at the same time: Yuzhniy Tsitadel which is supposed to grow mussels in the nearby coastal waters which owns extensive vineyards near the palace Local residents call the resort “Alina Kabaeva’s dacha,” referring to a rhythmic gymnast who is reportedly Putin’s longtime partner The reason for the rumored connection to her is unclear Leaked emails from Sibur’s top management reveal what it’s used for Sibur board member Marina Medvedeva was renting a guest cottage on the grounds of Strela resort For her 228-square-meter escape on the shores of the Black Sea she paid just 20,000 rubles ($270) per month The resort itself took care of all the upkeep: It bought curtains and furniture Medvedeva paid another 100 rubles — just over a dollar — every month Medvedeva said that the rent she paid was determined by market prices and that she paid additional on-the-spot fees that made up ”the majority” of her payments No such fees are mentioned in a rental agreement for her cottage that appeared in the leaked emails reporters counted 25 such cottages on the territory of the sanatorium but the resort is heavily guarded against prying eyes For clarification on what to do after Nogata’s email which they appear to have found perplexing “Act in accordance with the proposed recommendations?” one asked Konov promised to “ask.” Two days later he suggested that Sibur’s vice president who was still Putin’s son-in-law at the time Konov instructed Mikhailov: “we continue to pay.” Sibur employees noted that the new management was not letting them visit the villa which they were supposed to do periodically to ensure Sibur’s payments were justified “A preliminary answer was received from the new management company with a rejection [of our request] to visit the property,” a board member wrote to Konov a Sibur representative described the company’s arrangement as a “sublet” that gave it only limited non-exclusive access to the property between 2012 and 2013 He said Sibur used it for corporate events such as management seminars and team-building exercises and that the company had no information about its use by Putin He provided an additional response from Konov Sibur’s board chairman at the time of the rental who said the idea Sibur would carry out the president’s personal wishes was “absurd.” None of the documents seen by reporters describe the arrangement as a limited “sublet,” and the sign designating Villa Sellgren as a Sibur “recreation center” is difficult to square with such an arrangement the company representative said that the sign had not been placed by Sibur and noted that its layout and formatting did not match Sibur’s standard branding.) the day after Navalny released his investigation the leaked emails show Sibur executives strategizing about how to contain social-media chatter linking the company to the villa — which they referred to only as “the real estate.” but keeping in mind the fast organic growth of the comments around the video … our comments are not noticeable Attached to the email is a list of online comments defending Putin and Sibur “You’re not going to store your mushrooms and salt your fish in a shed :) :) :).” Support from readers like you helps OCCRP expose organized crime and corruption around the world you’ll be directly supporting investigative journalism as a public good You’ll also gain access to exclusive insights and benefits Vladimir Putin has always denied having any serious wealth But one of his relatives somehow amassed as much as $573 million.. An email domain not visible to the public — LLCInvest.ru — helped reporters uncover a group of interconnected companies that.. Trump cabinet member retains a stake in company that provides shipping for Putin son-in-law and sanctioned oligarchs Professional kickboxer and model Hitomi Nogata is looking to boost her internet fame by offering a literal kick in the pants to anyone willing, for absolutely free. Willing participants have their "kick ass" videos uploaded to Nogata's YouTube channel a 22-year-old representative of a Tokyo venture company He commented that the effect was very stimulating and while he wouldn't let Nogata kick him every day The female kickboxer took her talent to the Niconico Kaigi 2015 event in April where women and Nogata's own manager also volunteered for a motivating kick The market for these mildly masochistic activities have gotten more attention lately. Gravure idol Risa Yoshiki released a series of videos dedicating to berating the viewer in different situations and settings like a work environment and in Edo-era Japan Source: Nikkei Asian Review, Tokyo Kinky Japanese hostage in Iraq believed to be civilian traveler (Agencies) Updated: 2004-10-27 14:33 6,953 officials probed for power flop Statistics show rise in industrial profits Dissecting China's 'middle class' FM: talks with Japan on sea border advance AP: New Bush guard papers leave questions Israeli parliament backs Gaza pullout plan 'Aranumano' (for Araiyakushi, Numabukuro and Nogata stroll and drink in Aranumano: last but not least comes Numabukuro the liveliest and most surprising of these three 'hoods Japan refuses to withdraw troops from Iraq (Agencies) Updated: 2004-10-27 20:42 Making of anti-trust law is speeded up Housing price up 9.9% in major cities Powell's remarks consistent with US China policy Hi-tech private firms in China are booming One-China policy key to talks with Taipei Multinationals' fund flow gets easier A Korean college student in his 20s studying in Japan was arrested by local police on charges of sexual harassment of minors the Nogata Police of the Japanese Metropolitan Police Agency arrested a 23-year-old Korean international student A on charges of sexual harassment and is investigating him A is accused of touching the chest and patting the thigh of a middle school girl B for about an hour at her apartment in Nakano-gu is known to be an acquaintance of the woman A was dating A is known to have committed the crime when his girlfriend and B visited his house B reported the damage to the police in February A reportedly denied some of the charges in a police investigation "It is true that I touched the victim's chest ※ This service is provided by machine translation tool Nakano and Koenji are promoting exercise and healthy eating with contests This year has gone by in a blink of an eye with October already swooping in on a refreshing autumnal wind It’s the perfect month to be outside traversing undiscovered places in well-known neighborhoods Nakano is encouraging just that with a local treasure hunt and Koenji is promoting its ward with a festival filled with family activities Continue reading to find out what else these Western Tokyo hubs have in store for us in October Even in a sizeable city like Tokyo, it can be hard to find authentic lesser-known cuisine such as Sri Lankan food. This is where Spice Curry comes in they have created a nutritious curry that cuts out the fat but keeps in the good stuff the dishes come with a mix of wonderful flavors all on one plate One of the options is a traditional parippu curry (also known as dhal curry) which lacks gluten but not flavor A post shared by SWEET HOME SALAD (@sweethomesalad) Down the road in Nogata, you’ll find a new take-out shop. From the people behind the local Daily Coffee Stand, arrives Sweet Home Salad They had a soft opening which meant soups and side dishes were available early but customers had to wait until the grand opening on October 11 for the highly-anticipated salads made from chestnut pork and Russian-style beef The newly opened Gimbap One is providing a healthy and iconic Korean snack for the Koenji community these spicy seaweed rolls are jam-packed with nutritional ingredients that aren’t overpowered by rice try the melty cheese gimbap that harmonizes spicy and salty flavors Those watching their calories may opt for the egg gimbap which is a refreshing snack uncover the real flavors of Korea without traveling too far The hunt for international ingredients in Japan can be slightly laborious and expensive You have to know where to look and sometimes do the rounds in various small import shops This new supermarket in Nakano Broadway offers a lot of products in one place They provide a generous stock of food from China you can fill your baskets with various types of dumplings There’s also a broad selection of juices including coconut If you enjoyed the first group exhibition from the Mono.logues art space then you must attend the second installment: Unlogical 02 They will introduce artists Hayato Tobayama Mai Yokoyama and Sal who all vary in their use of mediums and motifs Tobayama focuses on abstract images and hidden figures while Yokoyama uses oil paints to explore what it means to be ‘genuine.’ Lastly One of the four major Koenji festivals, this two-day autumn event riles the townsfolk of Koenji into an extraordinary display of community you can participate in live musical performances stamp rallies and sales in the vicinity of Koenji station It’s a great chance to try the “Curry Battle of Suginami” where you can go head-to-head with other curry lovers Kosugiyu is a much-loved and long-established Koenji bathhouse known for its beautiful Mount Fuji mural It is currently holding a promotion with the new slogan: “Ato wa neru dake,” meaning “all that’s left now is go to sleep.” Beautiful illustrations will be exhibited during this period as well as a stamp rally for the popular relaxation drink Chill Out it might be time to go soak in the thermal waters of your local sento https://www.instagram.com/p/CTeh-f3HwFa/?utm_source=ig_web_copy_link Winners will be published on the official website and get souvenirs If you want to stay up to date with what’s new in Tokyo, Yokohama, Chiba and Kamakura, make sure to check out our What’s New section Elevate your thrifting game at these lesser known gems By Jamila Brown Thrift stores are as common as konbini (convenience stores) in Japan’s fashion capital. In Tokyo, entire districts like Shimokitazawa and Harajuku have blossomed into designated secondhand havens Due to the deep-rooted concept of mottainai (aversion to wastefulness) within Japanese culture and the fact that styles are cycling back into fashion faster than ever the demand for high-quality vintage clothes has been met with a seemingly endless abundance of thrift stores popping up across Japan Most J-fashion and lifestyle vloggers could walk you through all the big retail giants like Second Street, Kinji and New York Joe — all beloved mainstays of the Tokyo thrifting experience While those stores are a great jumping-off point for recycled fashion here are five lesser-known thrift stores in Tokyo to elevate your thrifting strategy.  with the most expensive item priced at ¥5,000 which can be a relief for those who prefer to shop without being in the leering eyes of shopkeepers making it less of an ideal option for those shopping in groups There isn’t a working website available so it can be difficult to find updates about the store.  A post shared by number me / ナンバーミー (@number_me_official) on Sep 13 Tucked away in the northern end of Shibuya, Number Me is a vintage thrift store that’s rooted in upscale aesthetics The style of the clothing here has the quality cut and sewing techniques rooted heavily in Japanese fashion design The store carries a variety of secondhand clothes for both men and women as well as its own brand of clothing.  Pros: The shop is conveniently located in Shibuya and carries a variety of carefully selected pieces to suit a more upscale Cons: With only one sign hinting at the location The prices are a little higher than what’s usually expected at a vintage shop.  Expect to Find: High quality Japanese and foreign brands including Beams A post shared by 古着屋FunnyFace ※東京都『田端』に移転🌈 (@usedfunnyface) on Sep 5 If you’re a fan of brightly colored dresses that look like they’ve jived straight of the 50s, you’ll find plenty at Used Funny Face in Arakawa Ward The shop’s vibrant collection is reflected in its friendly staff members Pros: The average price of the dresses are around ￥5,000 The staff are friendly and there are a variety of sizes available.   Cons: The location may feel a bit out of the way for some it’s a 10-minute walk to the store.  Expect to Find: Non-branded and Japanese brand dresses A post shared by TOKYO LAMPOON / EREVA (@tokyolampoon_ereva) on Sep 6 Thrifting wouldn’t mean much without the chance to bag yourself some high-end designer items at a fraction of their retail price. Tokyo Lampoon & Ereva offers a large selection of designer products from Gucci handbags to Dior earrings.  Pros: All items are certified designer brands at fair prices There’s an online shop to check for availability as well so you’ll have to be quick and decisive if you see something you like.  Tokyo is synonymous with cosplay trends, and for all the anime lovers out there Mitsubado Cosplay Shop is a great thrift store to find quality costumes at low prices Shoppers will be able to find anything from typical maid outfits to popular Naruto costumes and even some lesser-known princess costumes The price of a costume set generally ranges from ¥1,000 to around ¥10,000.  Pros: The shop is conveniently located in Akihabara and boasts great price points for cosplayers on a budget You’ll likely be able to find many popular cosplays as well.  The inventory is also highly dependent on current cosplay trends Not a large variety in sizes as most of the costumes are in Japanese sizes which typically run smaller than Western sizes.  From purple sweet potatoes to wild horse sightings here’s how to make the most of Okinawa’s tropical escape Explore the best local brands shaping Tokyo’s fashion scene From vintage clothing to Hawaiian-themed goods here’s what’s worth checking out before it’s gone From buying and registering a bike to key rules of the road Collagen staples for beauty and health in Japan Our handpicked list of the best events going on this month and the untold tales of Japan’s queer community Everything you need to know before and during a natural disaster Stay up to date with Tokyo news and events 'Aranumano' (for Araiyakushi, Numabukuro and Nogata stroll and drink in Aranumano: this second entry looks at the streets around Araiyakushi-mae Station Japanese version