AAPI ancestry/background: Japanese. Masahiro was born in Fukushima and came to the US (first to North Carolina) in 2004 to further his career in sports medicine. What he does for the Commanders: As the team's assistant athletic trainer, Masahiro is in charge of providing and coordinating all kinds of health care services for players, coaches, support and so many others within our organization. Much of his work focuses on treating and preventing injury and illness, promoting wellness and developing rehab and physical therapy programs. His favorite Japanese traditions: "A lot of stuff, but one of my favorite things in Japan was getting the whole extended family together and spending a couple days together during the New Year. New Years celebrations in Japan is like Thanksgiving in the US. I've definitely been missing that since living here, but the good thing is we still have FaceTime and Skype and everything." What AAPI Heritage Month means to him: "I only recently learned about AAPI Heritage Month actually, but I really appreciate how far our community has come and the contributions we've made. A lot of Asian flavors have been mixed in with our current American culture in so many different aspects." Favorite quote/mantra: "Teamwork makes the dreamwork" and "Control the controllables" Volume 9 - 2018 | https://doi.org/10.3389/fpls.2018.01163 This article is part of the Research TopicPolyploid Population Genetics and Evolution - From Theory to PracticeView all 13 articles Genome duplications aid in the formation of novel molecular networks through regulatory differentiation of the duplicated genes and facilitate adaptation to environmental change contains three homoeologous chromosome sets which evolved through interspecific hybridization and subsequent whole-genome duplication The divergent expression patterns of the homoeologs in hexaploid wheat suggest that they have undergone transcriptional and/or functional differentiation during wheat evolution the distribution of transcriptionally differentiated homoeologs in gene regulatory networks and their related biological functions in hexaploid wheat are still largely unexplored we retrieved 727 publicly available wheat RNA-sequencing (RNA-seq) datasets from various tissues and identified 10,415 expressed homoeologous triplets Examining the co-expression modules in the wheat transcriptome we found that 66% of the expressed homoeologous triplets possess all three homoeologs grouped in the same co-expression modules 15 triplets contain co-expressed homoeologs with differential expression levels between homoeoalleles across ≥ 95% of the 727 RNA-seq datasets suggesting a consistent trend of homoeolog expression bias we identified 2,831 differentiated homoeologs that showed gene expression patterns that deviated from those of the other two homoeologs We found that seven co-expression modules contained a high proportion of such differentiated homoeologs which accounted for ≥ 20% of the genes in each module We also found that five of the co-expression modules are abundantly composed of genes involved in biological processes such as chloroplast biogenesis the differentiated homoeologs might highly contribute to these biological functions in the gene network of hexaploid wheat These examples suggest that allopolyploidization often leads to increased productivity through fixation of genomic heterozygosity which improves environmental fitness and contributes to the habitat expansion of a species The differential employment of homoeologs through dynamic transcriptional regulation may contribute to the enhanced evolutionarily adaptability of allopolyploid species The divergent expression patterns between homoeologs suggest that they have undergone transcriptional and/or functional differentiation to elucidate homoeologous networks in hexaploid wheat and to explore their differentiation we retrieved publicly available RNA-sequencing (RNA-seq) datasets from various tissues We categorized hexaploid wheat genes to construct homoeologous groups and identified expressed homoeologous triplets We also identified differentiated homoeologs that show gene expression patterns that deviate from those of the other two homoeologs we explored gene network modules containing a high proportion of differentiated homoeologs in the transcriptome of hexaploid wheat We assessed enriched functions in the network modules and discussed the evolution of such network modules resulting from transcriptional differentiation of homoeologs in hexaploid wheat for which ≥ 50% of raw reads remained after the read removal step The reads per million mapped reads (RPM) values were calculated for all genes in the 727 read alignment datasets Genes with an RPM ≥ 3 in at least eight datasets (≥ 1% of the 727 RNA-seq datasets) were identified as significantly expressed genes and D-homoeologs that show high sequence similarity with the other two subgenomes based on BLAST analysis The e-value cut-off was set at 1e-5 and the sequence identity cut-off was set to 90% Values in brackets are percentages of the total number of query sequences (B) Proportions of genes classified into each homoeologous group ABD: sets of three homoeologs that are reciprocal best hits in all pairwise comparisons (i.e. and BD: sets of two homoeologs with reciprocal best hits for two subgenomes and without hits for the other subgenome (i.e. and D: genes without hits in any of the other two subgenomes (i.e. subgenome-unique genes); Others: genes that are not clustered into an homoeologous groups (e.g. genes with BLAST hits for the other subgenome(s) but that are not reciprocal best hits) The outer circle shows proportions of the number of expressed genes in each homoeologous group To summarize expression patterns of the genes with an RPM ≥ 3 in a range of 1–7 datasets (spatiotemporally expressed genes), t-SNE analysis was performed using the Rtsne package (v.0.13)3 in R (v.3.4.3) The number of iterations was set at 10,000 To compute co-expression modules of homoeologs, WGCNA analysis (Langfelder and Horvath, 2008) was performed based on the normalized RPM using the one-step automatic network construction method with the following parameters: power = 9 A soft-thresholding power was selected by evaluating the scale-free topology model fit Pairs of homoeologs with a false discovery rate (FDR) ≤ 0.001 and RPM (average of 2 biological replicates in the RNA-seq datasets) ≥ 3 for at least one of the homoeologs were identified as significantly differentially expressed homoeologs Spatiotemporally or non-significantly expressed genes in hexaploid wheat (A) Distribution of spatiotemporally or non-significantly expressed genes across the 21 wheat chromosomes (B) Proportion of the spatiotemporally expressed genes (RPM ≥ 3 in at least one RNA-seq dataset) and non-significantly expressed genes (RPM < 3 in all of the 727 RNA-seq datasets) in hexaploid wheat (C) t-SNE plot of the spatiotemporally expressed genes (D) Enriched GO terms in the biological processes of the spatiotemporally or non-significantly expressed genes in hexaploid wheat Co-expressed while differentially expressed homoeologs in hexaploid wheat (A) Proportion of co-expression patterns of homoeologs in the expressed homoeologous triplets homoeologous triplets in which all three homoeologs are grouped in the same co-expression module; AB-D homoeologous triplets in which A- and B-homoeologs are grouped in the same co-expression module while D-homoeolog is in another co-expression module; AD-B homoeologous triplets in which A- and D-homoeologs are grouped in the same co-expression module while B-homoeolog is in another co-expression module; BD-A homoeologous triplets in which B- and D-homoeologs are grouped in the same co-expression module while A-homoeolog is in another co-expression module; A-B-D homoeologous triplets in which all three homoeologs are assigned to different modules; Not clustered homoeologous triplets in which two or all three homoeologs are not assigned to a co-expression module (B) Number of co-expressed triplets containing differentially expressed homoeologs across ≥ 50% of the 727 RNA-seq datasets (C) Box plot of the expression levels of the homoeologs in 15 homoeologous triplets showing a consistent trend of homoeolog expression bias ≥ 95% across the 727 RNA-seq datasets (D) Enriched GO terms in the biological processes of genes in the 258 co-expressed triplets containing differentially expressed homoeologs across ≥ 50% of the 727 RNA-seq datasets These findings suggest that differentiated homoeologs might highly contribute to these biological functions in the gene network of hexaploid wheat Co-expression modules containing the differentiated homoeologs in hexaploid wheat (A) Number of homoeologs and differentiated homoeologs (numbers with their percentage in brackets) in each of the co-expression modules The Module 0 represents genes that are not clustered in a co-expression module Percentages represent proportions of the differentiated homoeologs in each of the co-expression modules (B–E) Enriched GO terms in the biological processes of genes in the co-expression module 7 (B) and 18 (F) projected to a 2D semantic space Circle size represents the –log10 of FDR values calculated using REVIGO analysis The top ten enriched GO terms are labeled in the plots our results suggest that along with other genes such differentiated homoeologs may have innovated transcriptional networks which may have contributed to adaptation to environmental change as well as to enhanced productivity during the evolution of hexaploid wheat provide us with an opportunity to further explore sub-/neofunctionalized homoeologs and elucidate the diploidization process that occurred during the evolution of hexaploid wheat after allopolyploidization Such analysis will enable us to identify genes and transcriptional modules that may be associated with adaptive traits in hexaploid wheat Such genes and transcriptional modules might also prove useful in enhancing the adaptation of staple crops to counter the potentially adverse impacts of global climate changes and improve their productivity KT and KI performed the bioinformatics analysis This work was partially supported by grants-in-aid for Young Scientists (A) (Grant No 26712003 to KM) from the Japan Society for the Promotion of Science (JSPS) and by funds to KM from the CREST (JPMJCR16O4) of the Japan Science and Technology Agency (JST) The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest The authors thank Shun Takaya for his help in data analysis This work was supported by RIKEN Junior Research Associate Program The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2018.01163/full#supplementary-material Evolution of duplicate gene expression in polyploid and hybrid plants Novel patterns of gene expression in polyploid plants On the relative abundance of autopolyploids and allopolyploids Functional divergence of duplicated genes formed by polyploidy during Arabidopsis evolution Trimmomatic: a flexible trimmer for Illumina sequence data transcriptional bias and functional divergence of homeologs in polyploid cotton (gossypium) Molecular mechanisms of polyploidy and hybrid vigor Mechanisms of genomic rearrangements and gene expression changes in plant polyploids Polyploidy: genome obesity and its consequences The advantages and disadvantages of being polyploid Contribution of subgenomes to the transcriptome and their intertwined regulation in the allopolyploid Coffea arabica grown at contrasted temperatures visualization and analysis in functional genomics research When gene marriages don’t work out: divorce by subfunctionalization Homeologous genes involved in mannitol synthesis reveal unequal contributions in response to abiotic stress in Coffea arabica Evolutionary genetics of genome merger and doubling in plants doi: 10.1146/annurev.genet.42.110807.091524 Genome plasticity a key factor in the success of polyploid wheat under domestication Genome evolution due to allopolyploidization in wheat Expression divergence of TaMBD2 homoeologous genes encoding methyl CpG-binding domain proteins in wheat (Triticum aestivum L.) The impact of widespread regulatory neofunctionalization on homeolog gene evolution following whole-genome duplication in maize International Wheat Genome Sequencing Consortium (2014) A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome PubMed Abstract | CrossRef Full Text | Google Scholar WGCNA: an R package for weighted correlation network analysis Patterns of homoeologous gene expression shown by RNA sequencing in hexaploid bread wheat Perspective – Genomic plasticity and the diversity of polyploid plants The impact of polyploidy on grass genome evolution Fast and accurate short read alignment with Burrows-Wheeler transform Draft genome of the wheat A-genome progenitor Triticum urartu Expression partitioning between genes duplicated by polyploidy under abiotic stress and during organ development Temporal transcriptome profiling reveals expression partitioning of homeologous genes contributing to heat and drought acclimation in wheat (Triticum aestivum L.) Genome sequence of the progenitor of the wheat D genome Aegilops tauschii The evolutionary fate and consequences of duplicate genes CrossRef Full Text | Google Scholar Polyploidy and its effect on evolutionary success: old questions revisited with new tools Evolution of polyploid triticum wheats under cultivation: the role of domestication natural hybridization and allopolyploid speciation in their diversification BLAST: at the core of a powerful and diverse set of sequence analysis tools Unlocking triticeae genomics to sustainably feed the future Discrimination of homoeologous gene expression in hexaploid wheat by SNP analysis of contigs grouped from a large number of expressed sequence tags The evolutionary dynamics of plant duplicate genes Rapid functional and evolutionary changes follow gene duplication in yeast Multiple mechanisms and challenges for the application of allopolyploidy in plants Genome interplay in the grain transcriptome of hexaploid bread wheat Multiple abiotic stimuli are integrated in the regulation of rice gene expression under field conditions The defence-associated transcriptome of hexaploid wheat displays homoeolog expression and induction bias Evolution of polyploidy and functional diploidization in sturgeons: microsatellite analysis in 10 sturgeon species CrossRef Full Text | Google Scholar and rates of polyploid formation in flowering plants Doubling down on genomes: polyploidy and crop plants edgeR: a Bioconductor package for differential expression analysis of digital gene expression data Environmental regulation of heterosis in the allopolyploid Arabidopsis suecica The role of hybridization in plant speciation doi: 10.1146/annurev.arplant.043008.092039 REVIGO summarizes and visualizes long lists of gene ontology terms Homoeolog-specific activation of genes for heat acclimation in the allopolyploid grass Brachypodium hybridum Homoeologous copy-specific expression patterns of MADS-box genes for floral formation in allopolyploid wheat The evolutionary significance of ancient genome duplications Transcriptome association identifies regulators of wheat spike architecture CrossRef Full Text | Google Scholar Transcript profiling provides evidence of functional divergence and expression networks among ribosomal protein gene paralogs in Brassica napus Evolution of physiological responses to salt stress in hexaploid wheat RNA sequencing of laser-capture microdissected compartments of the maize kernel identifies regulatory modules associated with endosperm cell differentiation Inoue K and Mochida K (2018) Gene Co-expression Network Analysis Suggests the Existence of Transcriptional Modules Containing a High Proportion of Transcriptionally Differentiated Homoeologs in Hexaploid Wheat Copyright © 2018 Takahagi, Inoue and Mochida. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) distribution or reproduction in other forums is permitted provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited in accordance with accepted academic practice distribution or reproduction is permitted which does not comply with these terms *Correspondence: Keiichi Mochida, a2VpaWNoaS5tb2NoaWRhQHJpa2VuLmpw Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher 94% of researchers rate our articles as excellent or goodLearn more about the work of our research integrity team to safeguard the quality of each article we publish The period of enrollment was only two and a half years his presence in the blue and red uniform as a high school graduate will undoubtedly leave a mark in the club's history.Kuryu MATSUKI 21 years old.The growth that led to becoming captain at a young age in Tokyo and the "path" left behind at the club.A writer who has been watching his journey and future in Kodaira every day as he sets off for Europe with the blue and red soul in his heart.Text by Kyohei Baba He was a player who embodied the concept of "Shuhari," which illustrates the process of training for those who aspire to follow the path.Kuryu MATSUKI has faithfully followed his teachings he has embarked on a new journey to establish his own unique world.I have continued to burn with an insatiable desire for improvement like something out of a boy's manga."Difficult or challenging moments can be enjoyable." he brushes his hair back and pats his cheeks to regain his determination That's how he opened the door to his dream He said that the biggest change before and after turning pro was "thinking while playing soccer.""At first I didn't think about it at all and just played without any thoughts but every time I heard stories from my seniors I was able to find ways to break through on my own."He showed a difference from the first year of his professional career at the camp If there was something he didn't understand he would catch his seniors or staff during practice or after practice and bombard them with questions "I thought I couldn't do it unless I did that much." It had become a daily routine in Odaira Yojiro TAKAHAGI was also one of the players who was often called out by Matsuki.Takahagi transferred to Tochigi SC in the J2 League in the summer of the 2022 season to find a place to shine and only played with Matsuki for six months when he visited Ajinomoto Stadium to greet everyone Matsuki approached him and asked with a serious look in his eyes I watched from above and talked about how it should be but Kuryu's drive to improve is amazing."He increased his professional learning and learned how to draw a growth curve Perhaps that's why there is a scent of the predecessors in Matsuki's play The long feed from his own goal that became the starting point for Ryotaro ARAKI's goal in the 4th round of the Meiji Yasuda J1 League against Avispa Fukuoka this season was very similar to the witty pass that Takahagi is known for Matsuki was also proactive in making changes where necessary."Shoelaces they are untied."Even though I didn't realize it myself It's because he's watching closely that he notices things.It's the same during interviews and then make eye contact with the other person to have a conversation They understood the questions and gave us comments that exceeded our expectations multiple times when we are impressed by their killer comments we naturally smile.That's why I've taken on a leading role in this team "I want to be a good example for my younger teammates as a representative of the young players," and I've made my position clear."I have a desire to push up from the younger generation I also have to do it as a leader of the younger generation."In his second year as a professional when asked about his responsibilities and awareness "The younger players are like 'follow me' to me," but I think half of it was a joke and half of it was serious He has taken on the role of connecting the upper and lower levels and has been working to create a team with good communication When Peter (Klamovski coach) honestly told me that he wanted me to be the captain I felt like I was in a position to lead this team but each of us played our roles in a well-balanced manner and Kei-kun communicated with various players creating a good balance." We have worked hard to enhance communication within the team instilling in each player on the pitch the awareness that they are all captains Their very presence has had a significant impact on those around them When Kota TAWARATSUMIDA and others were in their third year at FC Tokyo U-18 they were always inspired by Coach Takashi HARA (now a top team coach) the player who embodies Tokyo the most in the top team is Kuryu MATSUKI Shouldn't you academy players be like that?" They run and fight for the team Kuryu has been cherished by his seniors and has continued to walk the path he believes in as a good role model for his juniors he had become a player who truly suited the captain's armband After leaving behind many souvenirs in blue and red He fulfilled his dream of transferring to the English Premier League's Southampton FC and is now playing for Turkey's first division team Perhaps it was inevitable.Matsuki has been coming to the airport every time a player leaves to challenge overseas in the past two and a half years saying "because I am indebted to them" I am reminded that such players will follow in the future.""I have been able to play in many games and gain more experience than other players There are role models and younger players who are eager to compete That's why I have high expectations for all players I have received great support from everyone so I want to firmly follow the path I have chosen."The 2 and a half year journey shows the correctness of the path we have taken There are still many dreams we want to achieve Being selected for the Japan national team and even winning the first UEFA Champions League as a Japanese player there is a huge dream of becoming the greatest player among the players who have left FC Tokyo as promised with the blue and red singers."I believe that if you work hard your dreams will come true."The journey of Kuryu MATSUKI whose name contains the word "king" The protagonist will always take a strong step forward overcoming any difficulties in order to have the last laugh That is why he is the Kuryu MATSUKI we have seen A 15-year-old junior high school girl committed suicide last week after leaving a note accusing a male coach at her table tennis club of verbal abuse she wrote that the coach and teacher at her school had called students "idiots" and told them "I will kill you." He also made physical contact with several club members according to the Takahagi city education board in Ibaraki Prefecture (Officials of the Takahagi city education board apologize at a press conference held on May 6 2019 after the suicide of a 15-year-old junior high school girl.) The teacher is a veteran table tennis coach and the club has a track record of sending players to compete in national tournaments He largely admitted to the girl's allegations "The coaching had gone too far," the board said In a questionnaire distributed by her school the name of which the girl's family has requested be withheld "I enjoy going to school but club activities are boring She continued to attend the school but stopped going to the table tennis club from March 15 the education board received an anonymous phone call about the teacher prompting the school to give him a verbal warning and to begin monitoring him during club activities The teacher was also seen slamming a paddle on the floor when he became very angry "I've heard that the student was cheerful and energetic and saw things through to the end once she decided to do them," said Tomio Ouchi "Responsibilities of the school and the education board are very grave Though the school principal and others had warned the teacher after receiving complaints about his coaching they failed to extend sufficient care to students The board said it will set up a third-party panel involving a lawyer to investigate the cause of her suicide and to study measures to prevent a similar incident from occurring again one of the girl's friends told Kyodo News that she was also stressed about her relationship with her family before her death deputy leader of a nonprofit group tackling child suicide "It is necessary to examine how the school responded after exploring the cause of her suicide from various perspectives including her relationship with teammates and concerns about her family." To have the latest news and stories delivered to your inbox Simply enter your email address below and an email will be sent through which to complete your subscription Please check your inbox for a confirmation email Thank you for reaching out to us.We will get back to you as soon as possible Metrics details Barley is one of the founder crops of Old world agriculture and has become the fourth most important cereal worldwide Information on genome-scale DNA polymorphisms allows elucidating the evolutionary history behind domestication as well as discovering and isolating useful genes for molecular breeding Deep transcriptome sequencing enables the exploration of sequence variations in transcribed sequences; such analysis is particularly useful for species with large and complex genomes we performed RNA sequencing of 20 barley accessions comprising representatives of several biogeographic regions and a wild ancestor We identified 38,729 to 79,949 SNPs in the 19 domesticated accessions and 55,403 SNPs in the wild barley and revealed their genome-wide distribution using a reference genome Genome-scale comparisons among accessions showed a clear differentiation between oriental and occidental barley populations The results based on population structure analyses provide genome-scale properties of sub-populations grouped to oriental Our findings suggest that the oriental population of domesticated barley has genomic variations distinct from those in occidental groups which might have contributed to barley’s domestication mining of genetic diversity from the Asian landraces might allow the detection of valuable variations that can be used to improve the adaptability of barley against various environmental conditions as well as improve our understanding on the evolutional history of this crop Scalability and cost-effectiveness of RNA-seq methods allow in-depth analysis of polymorphisms found in the exome regions even in a species with a large and complex genome we estimated the population structure of domesticated barley accessions we revealed domesticated barley genomic diversity and population subdivision in a genomic context Barley accessions and transcribed regions identified using RNA-seq analysis (a) Geographic origin of the barley accessions used in this study. The origins were plotted on a map data made with Natural Earth (http://www.naturalearthdata.com/) through the QGIS 2.8.3 Wein interface (http://qgis.org/en/site/index.html) (b) Number of transcribed regions identified using RNA-seq analysis which were compared to those annotated using the Morex genome in Ensembl Plants (c) Number of transcribed regions expressed in each accession (FPKM ≥ 1) (d) Range of regions expressed across the 20 barley accessions Results of the present study suggest that comprehensive transcriptome sequencing and comparative analyses with related species are essential to further annotate TUs in the barley genome which might provide a genomic foundation to explore genetic polymorphisms in the genic regions RNA-seq analysis appears to be a feasible approach to compare transcribed regions among accessions and to allow a rapid and comprehensive acquisition of SNP data from accessions without requiring pre-designed tools such as sequence capture probes or primer sets for targeted re-sequencing that has a large genome and immature gene-structure annotation RNA-seq analysis of several accessions might allow accumulating genome sequence variations to elucidate on the diversity of the population Accuracy assessment of the RNA-seq-based SNPs and its comparison with exome-sequencing-based SNPs Proportion of RNA-seq-based SNPs also present in the exome-sequencing data obtained by Mascher et al.33 in H602 (a) and in J247 (b) Identical call A: SNPs consistent with exome-sequencing-based SNPs and called by all programs Identical call B: SNPs consistent with exome-sequencing-based SNPs and called by one or two program(s) Identical call C: SNPs shared with raw data of the exome-sequencing dataset Different call: SNPs inconsistent between the RNA-seq and the exome-sequencing datasets RNA-seq only: SNPs only observed in the RNA-seq dataset Several methods are currently available to carry out the discovery and genotyping of genome-scale polymorphisms These methods are mainly classified into pre-designed and de novo approaches: SNP chips and exome-sequencing are representatives of the pre-designed approach which require hybridisation probes or PCR primers to enrich targeted regions High-density SNP chips like Illumina’sTM Infinium beads chip can support ~1 million SNPs in a custom designed panel If a high-quality and well-annotated reference genome is available these pre-designed approaches allow genotyping validated SNPs and/or resequencing targeted regions with deeper coverage and RNA-seq are becoming popular approaches for de novo genotyping Although recent price decline in sequencing cost has made it possible to perform whole genome re-sequencing in population analysis and shallow sequencing depth often becomes a problem depending on the genome size of the target species GBS is a cost effective approach for genome-scale genotyping and has been widely used in several species being feasible for large genomes such as those of wheat and barley Although sequencing depth in GBS depends on the genome size of the target species this method enables reducing genome representation performing a cost-effective polymorphism discovery and genotyping genic and intergenic regions Because GBS does not always require a reference genome sequence it can be used in population analysis and to construct an initial genetic map in organisms whose genome has not been sequenced we applied RNA-seq to examine genome-scale polymorphisms among barley accessions Although RNA-seq approach only covers SNPs on transcribed regions of expressed genes in analysed tissues it is a cost-effective and well scalable method to analyse polymorphisms on expressed genes Such range of SNPs might be useful for genome-wide genotyping at middle-scale density between amplicon-sequencing of panelled target genes and capture-based exome-sequencing densities Our data suggested that sequencing a pooled RNAs sample is efficient to avoid tissue dependency and to increase the number of sequenced regions and identified SNPs RNA-seq does not always require a reference genome as a de novo transcriptome assembly might serve as proxy for a reference genome enabling reducing genome representation without depending on its size and restriction sites RNA-seq and GBS appear to be reliable approaches to rapidly acquire genome-scale polymorphisms in organisms without reference genome information and mature structural annotation RNA-seq might also be an efficient approach to rapidly acquire genome-scale polymorphism datasets at the population scale using a core-collection or sub-populations of landraces and mapping populations Genome-wide distribution of the SNPs identified using RNA-seq analysis of the 20 diverse barley accessions (a) Distribution of the RNA-seq-based SNPs of the 20 barley accessions The numbers of SNPs were computed for each chromosome using 5 million bases sliding windows (b) Distribution of the bi-allelic RNA-seq-based SNPs (c) Genome-wide sequence identity based on the bi-allelic SNP dataset across the 20 barley accessions and Morex (d) Neighbour-joining tree of the 20 barley accessions and Morex based on the bi-allelic SNP dataset Molecular phylogeny and population structure according to the integrated SNPs dataset derived from RNA and exome-sequencing in barley (b) PCA plot of the 31 domesticated barley accessions and three wild barley accessions (c) PCA plot of the 31 domesticated barley accessions (d) Bayesian clustering of the 31 domesticated barley accessions using K = 2 (upper) and K = 7 (lower) populations Overview of the genomic diversity in domesticated barley sub-populations (a) Genome-scale statistics and LD blocks of domesticated barley sub-populations based on the bi-allelic SNP dataset (1) Haplotype diversity calculated for the occidental (red) and marginal (blue) barley populations using sliding windows of 5 million bases (2–4) Pairwise FST values calculated for the occidental and oriental groups (2) occidental and marginal groups (3) and oriental and marginal groups (4) using sliding windows of 5 million bases (5) LD blocks (≥10 kb) of occidental (red) and oriental (green) barley sub-populations (6) Density distribution of transcribed regions for the integrated dataset comprising annotated genes and the putative novel transcribed regions identified in the RNA-seq analysis (b) LD decay plots for the occidental (red) and oriental (green) barley sub-populations The average r2 values were plotted against physical distance The genetic patterns of differentiation of the Asian landraces of oriental barley might elucidate on the potential genetic adaptability of barley to the East Asian monsoon area The genome-wide variation determined using the RNA-seq technology might become an established tool to address future food security issues Our results suggest that oriental landraces and/or Asian barley might be important for the future increase of barley productivity The barley plants used for RNA extraction were grown using hydro-culture in a growth chamber The growth chamber was maintained at 16-h light: 8-h darkness at 22 °C First leaf blades (L1) and the remaining parts of shoots (L2) were separately harvested at 14 days after germination and Rt from each barley accession was evenly pooled All RNA samples were assessed using the Agilent 2100 bioanalyzer (Agilent Technologies USA) using the Agilent RNA 6000 Pico Kit (Agilent Technologies Total RNAs were stored at −80 °C until library preparation for RNA sequencing Poly(A) + RNAs were purified using MicroPoly(A)Purist™ Kit (Life Technologies according to the manufacturer’s instructions and assessed using the Agilent 2100 bioanalyzer (Agilent Technologies USA) and the Agilent RNA 6000 Pico Kit (Agilent Technologies Libraries for strand-specific RNA sequencing were obtained in an Ion torrent sequencer using the Ion Total RNA-Seq Kit v and Ion Xpress Barcode Adapters (Life Technologies Templates for the 200 base-read libraries sequenced using the Ion Proton semiconductor sequencer (Life Technologies USA) were prepared using the Ion P1 Template OT2 200 Kit v Sequencing was performed using an Ion Proton sequencer (Life Technologies using the basic local alignment search tool and a translated nucleotide query (BLASTx The cufflinks command with –G option was used to calculate FPKM values based on the merged gene annotations including previously annotated genes and the putative novel transcribed regions for each accession Regions with FPKM ≥ 1 were defined as expressed genes or regions The intersectional SNPs identified by each of SNP callers that fulfilled the thresholds of read depth and allele frequency were subjected to further analyses as a set of reliable SNPs The uniquely mapped and properly paired reads were used in further analysis Possible duplicated reads were removed using the rmdup command in samtools The SNPs of the exome-sequencing data were identified using the same methods as in RNA-seq-based SNPs discovery Bi-allelic SNP datasets were constructed based on the SNPs that met the following criteria: (i) The SNP was detected in at least one accession; (ii) read depth of the SNP position was≥ 5 in all accessions; and (iii) other nucleotides were found at the SNP position in <10% of all accessions Average r2 values for each physical distance were calculated using original Perl scripts LD blocks in occidental and oriental barley sub-populations were found using Haploview with the setting -blockoutput GAB -nogui The LD blocks found were superimposed on the Morex reference genome using circos Accession codes: The read data have been submitted to DDBJ Sequence Read Archive [DDBJ: DRA003931] Analysis of single nucleotide polymorphisms based on RNA sequencing data of diverse bio-geographical accessions in barley Barley: a translational model for adaptation to climate change Climate change impacts on global food security Genetics and geography of wild cereal domestication in the near east The importance of barley genetics and domestication in a global perspective Measuring grain size and identifying Near Eastern cereal domestication: evidence from the Euphrates Valley From the PPNA to the PPNB: new views from the southern Levant after excavations at Zahrat adh-Dhra‘2 in Jordan Archaeobotanical research at Pre-Pottery Neolithic A sites in Jordan 119–128 (Department of Antiquities of Jordan reveals multiple independent domestications of cultivated rice Genetic evidence for a second domestication of barley (Hordeum vulgare) east of the Fertile Crescent F1 hybrid weakness in the common bean: differential geographic origin suggests two gene pools in cultivated bean germplasm Phaseolin-Protein Variability in Wild Forms and Landraces of the Common Bean (Phaseolus vulgaris): Evidence for Multiple Centers of Domestication Molecular analysis of the parallel domestication of the common bean (Phaseolus vulgaris) in Mesoamerica and the Andes Mesoamerican origin of the common bean (Phaseolus vulgaris L.) is revealed by sequence data Multiple domestications do not appear monophyletic Genes and mutations underlying domestication transitions in grasses Evolutionary history of wild barley (Hordeum vulgare subsp spontaneum) analyzed using multilocus sequence data and paleodistribution modeling Genetic resources of wild barley in the Near East: structure Two genomic regions contribute disproportionately to geographic differentiation in wild barley Molecular phylogeography of domesticated barley traces expansion of agriculture in the Old World The origin and evolution of cultivated barley Resequencing data indicate a modest effect of domestication on diversity in barley: a cultigen with multiple origins Barley landraces are characterized by geographically heterogeneous genomic origins Advances in omics and bioinformatics tools for systems analyses of plant functions Genomics and bioinformatics resources for crop improvement A high-density transcript linkage map of barley derived from a single population Developing genomic resources in two Linum species via 454 pyrosequencing and genomic reduction The USDA barley core collection: genetic diversity and potential for genome-wide association studies Ascertainment biases in SNP chips affect measures of population divergence Comparative analyses identify the contributions of exotic donors to disease resistance in a barley experimental population Barley whole exome capture: a tool for genomic research in the genus Hordeum and beyond Effects of ascertainment bias and marker number on estimations of barley diversity from high-throughput SNP genotype data Detection of single nucleotide variations in expressed exons of the human genome using RNA-Seq Natural variation of barley vernalization requirements: implication of quantitative variation of winter growth habit as an adaptive trait in East Asia Large-scale collection and analysis of full-length cDNAs from Brachypodium distachyon and integration with Pooideae sequence resources Genome diversity in Brachypodium distachyon: deep sequencing of highly diverse inbred lines Low concordance of multiple variant-calling pipelines: practical implications for exome and genome sequencing Evaluation of variant identification methods for whole genome sequencing data in dairy cattle Steps to ensure accuracy in genotype and SNP calling from Illumina sequencing data Review of alignment and SNP calling algorithms for next-generation sequencing data Development of high-density genetic maps for barley and wheat using a novel two-enzyme genotyping-by-sequencing approach A high density GBS map of bread wheat and its application for dissecting complex disease resistance traits Improvement of barley genome annotations by deciphering the Haruna Nijo genome Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study Estimation of levels of gene flow from DNA sequence data Recombination and linkage disequilibrium in Arabidopsis thaliana Whole-genome sequencing reveals untapped genetic potential in Africa’s indigenous cereal crop sorghum Resequencing 50 accessions of cultivated and wild rice yields markers for identifying agronomically important genes Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection The Sequence Alignment/Map format and SAMtools Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation Haplotype-based variant detection from short-read sequencing A map of human genome variation from population-scale sequencing BEDTools: The Swiss-Army Tool for Genome Feature Analysis MEGA6: Molecular Evolutionary Genetics Analysis version 6.0 Principal components analysis corrects for stratification in genome-wide association studies Inference of population structure using multilocus genotype data PopGenome: an efficient Swiss army knife for population genomic analyses in R Circos: an information aesthetic for comparative genomics Haploview: analysis and visualization of LD and haplotype maps Download references The authors thank the National BioResource Project (NBRP: Barley) for providing barley materials This work was supported by the IPSR Joint Usage/Research Center Research Grant (No 2641) awarded to K.M and D.S and by Grants-in-Aid for Scientific Research (B) (Grant No 15KT0038 awarded to K.M and D.S.) from the Japan Society for the Promotion of Science (JSPS) The authors also thank the research support grant for research staff with family responsibilities in RIKEN awarded to Y.U.Y Biomass Engineering Research Division,RIKEN Center for Sustainable Resource Science Yukiko Uehara-Yamaguchi & Keiichi Mochida Integrated Genome Informatics Research Unit RIKEN Center for Sustainable Resource Science contributed to biological interpretation of the results All authors read and approved the final manuscript The authors declare no competing financial interests Download citation Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing newsletter — what matters in science Acquires spectrum on the tech giant's Canada-Japan cable Fiber optic network provider Mox will join the Topaz subsea cable The carrier has acquired spectrum from Google, as reported by Capacity Media. First announced in April 2022 the Google-owned Topaz subsea cable system connects Asia and Canada with landing points in Port Alberni and Vancouver in Canada Topaz features 16 fiber pairs and a total capacity of 240Tbps Mox will use its existing network to connect data hubs along the Topaz route “We are thrilled to join Topaz as a commercial network provider offering high-capacity diverse networks connecting Canada and Japan,” said Allen Meeks “With the constantly evolving and insatiable focus on technology and AI our network infrastructure and international subsea systems are integral to supporting these innovations.” director of global submarine networking at Google added: “By offering fiber and spectrum to carriers like Mox Google ensures that other customers can benefit directly from the work we are doing to improve global connectivity with increased reach Google’s cable uses advanced Wavelength Selective Switch (WSS) technology; a software-defined method used for spectrum slicing WSS increases resilience and enables routing flexibility The Topaz cable also passes alongside Indigenous territories in Canada Its current customer base includes hyperscalers and wireless providers Mox’s network currently spans more than 16,000km across North America and Japan The company provides dark fiber channels and 400G wavelengths to wholesale customers Other cables landing in Canada include the Connected Coast set to connect Guam and the Commonwealth of the Northern Mariana Islands with Japan Data Centre Dynamics Ltd (DCD), 32-38 Saffron Hill, London, EC1N 8FH Email. [email protected]DCD is a subsidiary of InfraXmedia there is a unit out at OrthoVirginia Training Center at Commanders Park that is running This corps is not made up of football players but rather athletic trainers "I think if we were to check some step-counters on ourselves we're north of 20,000 miles I'm sure," Commanders' assistant head athletic trainer Mark McCracken said Washington's athletic training staff is hustling because they are putting in non-stop serious work during preseason to meet the demands of a grueling time of year To ensure they are able to provide the best care to the team throughout these intense weeks constantly keeping their heads on a swivel working hard and leaning on the camaraderie they have developed within the department is vital Putting themselves in the position to provide the required care comes with first understanding the extreme physical impact of training camp and preseason on the players conditioning and working out with their individual coaches in the lead up to this period but that cannot fully prepare their bodies for what summer with the team "The fact of the matter is they might be training decent amount when they come into training camp but nothing will prepare you for football like football the Commanders' Director of Physical Therapy Add in the shift that NFL play has experienced over recent years and it is no wonder that concerns about load are top of mind for the training room You see players are completely different athletes – faster stronger – than they were decades ago," said assistant athletic trainer Masahiro Takahagi (also known as Hagi) The Commanders' head athletic trainer Al Bellamy has witnessed many of those changes at pro level firsthand as he has been working in the league since the late 1980s He has been through dozens of training camps and could not overemphasize the idea that "the recovery process is vital for getting through this portion of the season." All members of the Commanders athletic training staff as well as those in the strength & conditioning department play key roles in helping the guys re-cooperate and re-charge after long sessions and back-to-back days in the unforgiving heat of Virginia summer making sure to weigh players before and after practice to see what is needed to get them re-hydrated uses GPS monitoring to help track player loads And then there is the recovery mecca nestled in the back of the Commanders athletic training room It becomes a sort of sacred space during training camp the kind of environment that feels as if the opening of its doors should come with a chorus of angels singing players have access to a number of high-tech devices that assist in recovery "Last few weeks this has definitely been a popular nap location," Hagi said with a smile which means players do not always have time to get down to the recovery room the trainer knows they always need to be adapting to and overcoming the hectic schedule to deliver guys what they need "During meetings we like to try to make that a recovery space We have some mobile modalities that we can give them in there," Hagi said just utilize any time to get them better."  This scenario also highlights one of the trainers' unofficial mantras: take the guess work out of life for the guys whenever possible because preseason alone is incredibly taxing Moments where this slogan is being ascribed to can be identified wherever the trainers are a subtle but important gesture that every trainer can be seen doing out at practice The athletic training staff members do not just hold water bottles and wait for the players to make eye contact with them to hand it over they are constantly offering water bottles to the players at their eye level There are physical demands and there's mental demands so we want to make sure we're taking care of that for them," McCracken explained Operating with this mindset for 90 players and over a dozen coaches all day while their job is to (at times quite literally) fill up the cups of the players they also need to find bits of time and space to fill up their own cups Many trainers try to squeeze in workouts whenever they can Music and even trivia are played often during treatment McCracken stressed the desire for the training room to "not be like a hospital setting." Turning to one another on staff also helps make sure the work is high quality "If someone's having a hard day or maybe they have a case with an athlete that they're struggling with Being there for each other is as important to the job as having a stim or rolls of athletic tape on hand They wear earpieces at practice to talk to each other Shared digital platforms provide streamlined ways for everyone on the athlete wellness and player performance side to stay looped in on players' statuses Hagi described it as a real "family" environment All of them know that together – with their different accreditations and strengths – they can offer up the kind of hard work and knowledge that will help the Commanders get to where they need to be and we need to provide that service accordingly," Ruiz said "Being able to help someone go out and achieve their dream and provide for their families A morning roundup of content from the Washington Commanders on Monday The Commanders rounded out their draft class by taking Jacory Croskey-Merritt Here are five things to know about the newest member of the backfield A morning roundup of content from the Washington Commanders on Friday The Washington Commanders added depth to their linebacker room by taking UCLA's Kain Medrano with the 125th overall pick Here are five things to know about the rookie and while they didn't get another pass-rusher they like they players they got in their five-person class The Commanders added depth to their wide receiver room with Jaylin Lane in the fourth round Here are five things to know about the newest pass-catcher A morning roundup of content from the Washington Commanders on Wednesday Here's what The Athletic's Dane Brugler had to say about each play in this year's version of "The Beast," an annual draft preview that provides in-depth analysis of hundreds of prospects each year a fifth-round pick by the Tampa Bay Buccaneers in the 2019 draft comes to Washington after spending two seasons with the Indianapolis Colts The Commanders used their second-round pick to take Ole Miss cornerback Trey Amos Here are five things to know about their newest defensive back $2.7 Billion Investment from the Commanders is the Single Largest Private Investment in District History Washington had the second smallest haul in the draft managed to fill needs deepen positions and get highly-rated players fit into their culture the teams have been drafted and the line has been drawn heading into Louisiana Tech's T-Day Spring Game Saturday at Joe Aillet Stadium at 1 p.m Louisiana Tech's football staff meeting room turned into a War Room Tuesday night following the Bulldogs 13th practice of the spring as head coach Derek Dooley sat at the head of the table surrounded by this year's senior-class-to-be The task in front of those Bulldog players .. to draft their teammates for this weekend's event which is free and open to all LA Tech fans "I feel really good about our team," said Graham who served as the official spokesman during the draft for the White Team "We drafted a lot of guys off the starting defense and we got Ross at quarterback who is 2-0 in these games so far "We have pretty much the entire starting defensive line who I think is going to dominate that Randy guy or whoever is starting at quarterback for them." Be looking for me to run all over their defense." Porter and Co. countered their opposition's selection of Jenkins by selecting his normal snapping partner in starting center Lon Roberts with their first pic It was the first of a number of strategic selections during the hour-long event Graham and the White Team's strategy was to keep Porter -- a first team all-WAC selection last year -- in check by drafting a majority of the Bulldogs starting defensive linemen, including Mason Hitt and Matt Broha who will also team up with Smith and Jordan up front "I know Porter is probably talking a lot of trash about how many touchdowns he is going to score but I think our defense is going to shut him down," Graham said Smith agreed with his teammate's assessment "We got pretty much the entire D-Line on our team and I think three out of the five starting offensive lineman and that s where it starts support personnel and athletic administrators Nick White and Sean Fox who will serve as the guest media head coaches were also selected as White will lead the White Team while Fox will walk the sideline for the Red Team "I feel especially good about our head coach Sean Fox," Porter said He's a real cool guy on the radio so we're excited to have him." "I'm real excited about our coach Nick White," Graham said "He's not afraid to tell it like it is and that's what we need in our head coach I know him and Fox get into it on the show a lot and I think Nick is going to come out on top in this one "I think he has a good idea about the game and I think he knows how to handle the pressure of being a head coach better than Fox does." The contest will be comprised of four 10-minute quarters with a 15-minute halftime Season ticket for the 2009 Bulldog season are on sale and can be purchased by calling 318-257-3631 emailing techtickets@latech.edu or logging onto www.latechsports.com 3 Randy Hardin QB 4 Tarence Calais DB 5 Dale Wallace K 7 Steven Ensminger QB 9 Cruz Williams WR 11 DeMarcion Evans DB 16 Darrius Terry DB 18 Adrian Linwood WR 19 Sumner Ellis DB 20 Daniel Porter RB 24 Andrew Guillot RB 29 Zach Booker RB 32 Joel Hall K 34 Antonio Baker DB 35 Olajuwan Paige CB 36 Brian White LB 40 Kiamni Washington LB 42 Tristan Broussard FB 44 Adrien Cole LB 48 Dusty Rust LB 49 Dennis Morris TE 59 Kevin Saia OL 60 Lon Roberts OL 61 Jared Barron DL 64 Corbin Best OL 72 Will Taylor OL 75 Michael Lacy OL 76 Cudahy Harmon OL 79 Kris Cavitt OL 83 Adrian Logan DE 85 Randy Grigsby DL 86 Eric Fiege WR 87 Clayton Chambers TE 92 Philip Longino DL 93 Christian Lacey DL 94 Ramone Randle DL Ed Jackson -- Strength and Conditioning Missy Farrar -- Student Success Specialist Leslie Kiletico -- Recruiting Office Coordinator Mason Ellenberger -- GM LA Tech Sports Properties Lori Hamilton -- Assistant to Athletic Director & COO Dana Petitt -- Athletics Accounting Technician Zach Williams -- Assistant AD/Ticket Operations 1 Richie Casey WR 5 D'Anthony Smith DL 6 Phillip Livas WR 8 Shawn Simmons S 8 Tym Toussaint DB 10 John Hinton K 10 Colby Cameron QB 10 Lamar Haddox WR 11 Ross Jenkins QB 14 Cade Glasgow K 15 Josh Victorian DB 18 Todd Pfaffenberger K 20 Alex Anglin DB 21 Dominique Faust LB 24 Todd Spence DB 25 Deon Young DB 26 Myke Compton RB 28 Terry Carter DB 30 Drexel Perkins DB 32 Allen Gilbert RB 39 Roosevelt Falls FB 41 Cornelious Nickelson RB 43 Dustin Mitchell TE 45 Jay Dudley LB 50 Jacob Miller OL 52 Thomas Graham DS 53 Adam Hymel OL 56 Solomon Randle LB 62 Ben Harris OL 63 Steven Warner OL 67 Jared Miles OL 68 Chris Tusch OL 73 Josh Parrish OL 74 Rob McGill OL 80 Houston Tuminello WR 83 R.P. Stuart WR 89 Chandler Spence TE 90 Mason Hitt DL 91 Matt Broha DL 96 John White DL 97 Kwame Jordan DL 98 Eric Harper LB Damon Harrington -- Strength and Conditioning Aaron Lips -- Strength and Conditioning Intern Kyle Manley -- Strength and Conditioning GA Bradley Choate -- Student Equipment Manager Greg Greathouse -- Student Video Coordinator Linda Lewis -- FB Operations Office Coordinator Marvin Roberts -- Student Video Coordinator Masahiro "Hagi" Takahagi -- GA Athletic Trainer Aaron Thacker -- Assistant Video Coordinator Natalie Fasullo -- Student Academic Assistant Malcolm Butler -- Associate AD/Media Relations Mary Kay Hungate -- Deputy Athletic Director Josh Perot -- Account Executive/LA Tech Sports Properties Thanks for visiting The use of software that blocks ads hinders our ability to serve you the content you came here to enjoy We ask that you consider turning off your ad blocker so we can deliver you the best experience possible while you are here Fixtures & Results, Trainings Players & Staff Stadium Hometown Activities Frequently Asked Questions Yojiro TAKAHAGI achieved his 300th appearance in the Meiji Yasuda J1 League Sec 22 match against Shimizu S-Pulse.To commemorate this we will be accepting orders for Yojiro TAKAHAGI player J1 League 300 games played commemorative goods.The product details and sales overview are as follows Please take this opportunity to make a purchase □Product Lineup1.Yojiro TAKAHAGI 300 Match Appearance Commemorative Replica Jersey*This is a limited product available only at the official online shop and Euro Sports Ajinomoto Stadium store.※There is a limited production quantity so it is possible to end the acceptance even during the order period so please be aware in advance.【Price】25,000 yen (excluding tax)【Size Range】SS-S 【Notes】(1) This will be signed by Yojiro TAKAHAGI.(2) Please note that due to the fact that the autograph is handwritten by the player himself Thank you for your understanding.(3) Please note that the design may change so please be aware in advance.(4) This product is limited to one purchase per person In the event that multiple purchases are discovered we may cancel the purchase.(5) If you purchase at the official online shop you can only use credit card payment.(6) There is a limit to the production quantity so it may be possible to end the acceptance even during the order period Yojiro TAKAHAGI 300 Games Played Commemorative Photo Frame 【Price】8,000 yen (excluding tax) 【Size】Approximately W360mm×H225mm【Notes】(1) The signature will be a printed design not handwritten.(2) Please note that the design and specifications may change in some cases Yojiro TAKAHAGI 300th Game Commemorative Uniform Bear 【Price】3L Size: 7,000 yen (excluding tax)L Size: 3,500 yen (excluding tax) 【Size】3L Size: Length approximately 350mmL Size: Length approximately 250mm【Notes】Please note that the design and specifications are subject to change Yojiro TAKAHAGI 300 Match Commemorative Neck Gaiter 【Price】2,000 yen (excluding tax) 【Size】Approximately W250mm×H500mm【Material】96% Polyester 4% Spandex[Notes]Please note that the design and specifications may change □Gift plan from Yojiro TAKAHAGIYojiro TAKAHAGI player himself has offered to hold a giveaway where 10 lucky winners who have purchased any of the Yojiro TAKAHAGI player's 300-game commemorative goods will receive a signed item by Yojiro TAKAHAGI player through a lottery!The winning prize will be sent to you ※The announcement of the winners will be made by shipping.※Signed goods cannot be selected so please understand in advance.□ Sales[Sales Location]・Official Online Shop ・Official Shops: 6 Locations(Euro Sports Ajinomoto Stadium Store, GALLERY・2 Shinjuku Store, GALLERY・2 Shibuya Store, Soccer Shop KAMO Harajuku Store, Soccer Shop KAMO Shinjuku Store, FC Tokyo Park Kodaira Tenjin)◇For details on the official shops, click here[Order Period]From Oct 16 2020 (Sun) 23:59[About product delivery]Scheduled for early December 2020 Announcement of New Product Sale on Wednesday 2025 FC Tokyo "May VICTORY AUCTION" Announcement Notice of merchandise booth at Niigata match on 5/3 (Sat Holiday) Shimizu Match Goods Shop & Gachapon Corner Announcement Announcement of merchandise sales commemorating Kei KOIZUMI's 300 J1 League appearances [4/25 Update] Announcement of new product sales on April 23 (Wednesday) Notice of merchandise sales and gacha corner for the G Osaka match on 4/25 (Friday) One legacy of the Fukushima nuclear disaster last year has already become apparent through a study of butterflies in Japan: Their rate of genetic mutations and deformities has increased with succeeding generations "Nature in the Fukushima area has been damaged," said Joji Otaki a professor at the University of the Ryukyus in Okinawa The abnormalities, which the researchers traced to the radiation released from the nuclear power plant, include infertility, deformed wings and the inability to fight their way out of their cocoons The butterflies from the sites with the most radiation in the environment have the most physical abnormalities "Insects have been considered to be highly resistant to radiation "It is quite concerning to see accumulated effects occurring over relatively short time periods Get the world’s most fascinating discoveries delivered straight to your inbox pale grass blue butterflies (Zizeeria maha) were overwintering as larvae Otaki and his colleagues collected adult butterflies from 10 locations They observed changes in the butterflies' eyes The researchers had been studying the pale grass blue butterfly for more than 10 years The insects live in the same places as people – gardens and public parks – which make them good environmental indicators and they are sensitive to environmental changes The team also bred the collected butterflies at the university's labs in Okinawa 1,100 miles (1,750 kilometers) from Fukushima They noticed more-severe abnormalities in successive generations such as forked antennas and asymmetrical wings The September butterflies were likely fourth- or fifth-generation descendants from the larvae present in May It is likely that the first generation of butterflies suffered both physical damage from radiation sickness and genetic damage from the massive exposure to radioactive isotopes after the disaster This generation passed on their genetic mutations to their offspring who then acquired their own genetic defects from eating radioactive leaves and from exposure to low levels of radiation remaining in the environment The cumulative effect caused successive generations to develop more serious physical abnormalities "Note that every generation was continuously exposed," said Otaki "This study adds to the growing evidence that low-dose radiation can lead to significant increases in mutations and deformities in wild animal populations." The findings are consistent with previous studies in Japan and at Chernobyl "The ecological studies that we have conducted found that the entire butterfly community in Fukushima was depressed in radioactive areas and that the patterns seen in Fukushima were similar to what has been observed in Chernobyl If the plants and animals are mutating and dying this should be cause for significant public concern." Editor's note: This story was updated at 5:20 p.m to correct the spelling of Timothy Mousseau's name.  La Niña is dead — what that means for this year's hurricanes and weather El Cono: The mysterious sacred 'pyramid' hidden deep in the Amazon rainforest Digital 'resurrection' of the Titanic sheds light on fateful night the ship tore apart Spiranovic greets Wanderers' big signing Popovic Wanderers stars Juric and Covic among 10 gone Giro d’Italia and Vuelta a España.Get the latest with our sport podcasts on your favourite podcast apps.Watch on SBS SportSport News making it easy to decorate even at home!Let's cheer for the players at the stadium!【Price】1,320 yen (including tax)【Size】80cm×80cm□FC Tokyo【Custom】NB Pile Cap '22This is a cap made of soft pile material that suggests a more casual viewing style!With the blue and red colors on the back of the cap and the blue and red tag on the back you can subtly show your club love!!【Price】4,180 yen (including tax)【Size】Head circumference 58cm (adjustable)【Material】80% Polyester 20% Cotton□ FC Tokyo【Custom】NB Reversible Hat '22It is a reversible hat that allows you to switch styles according to the scene OFF (black) while moving and ON (blue-red) while watching the game switch it to ON and dye Ajinomoto Stadium blue and red!【Price】5,060 yen (including tax)【Size】Head circumference 58cm【Material】80% Polyester 20% Cotton□FC Tokyo【Custom】NB Adventure Hat '22Outdoor hats which are gaining renewed attention due to the outdoor boom have arrived!The product is designed to block the sunlight and can be used not only for watching games but also for everyday activities such as picnics and supporting children's games!【Price】5,390 yen (including tax)【Size】Head circumference 58cm【Material】100% Polyester□Stadium Goods Store Limited Project Let's Win and Get It if you purchase over 1,500 yen (including tax) at the home side goods store inside the stadium you will receive one Victory Coin as a gift!!Stack up victories and collect coins! It is also recommended as a commemoration of watching the game we will give you one ticket at the home team goods store inside the stadium when you purchase 1,500 yen (tax included) or more.※1 ticket will be distributed for purchases of 1,500 yen or more (including tax).※Excluding gachagacha.※Distribution will end as soon as it runs out for each game.【Sales Locations】M Merchandise StoreN42 Merchandise StoreB37 Merchandise Store【Sales Hours】Mar 12 2022 (Sat)Doors open until 20 minutes after the end of the game ※Distribution of Victory Coins will only be available after the end of the winning game "Players Gachagacha Shop"If there is a long wait or congestion due to trading near the checkout line or Gachagacha corner we may cancel future sales.Please prepare 100 yen coins in advance as we will be using capsule vending machines.【Sales Location】Gachapon Corner near Main Side M9【Sales Hours】 From gate opening to 20 minutes after the end of the game sales will end as soon as each item is sold out 【Sales Method】 Please prepare 100 yen coins in advance and come to the venue as we will be selling capsules using a gachapon machine.【Notes】 (1) Each individual is limited to a maximum of 5 purchases per item (2) We cannot accept exchanges or returns due to customer convenience (3) Please line up in the representative waiting queue only trading of goods around the Gacha Gacha corner is prohibited If long delays or congestion due to trading are observed near the checkout queue or the Gacha Gacha corner future sales may be suspended.(5) In case of bad weather "Character Gacha Gacha Shop" Please prepare 500 yen coins in advance as the capsules will be sold using Gacha Gacha machines 【Sales Location】 Gachagacha Corner near N47【Sales Hours】 From gate opening to 20 minutes after the end of the game 【Sales Method】 Please prepare 500 yen coins in advance and come to the venue as we will be selling capsules using a gachapon machine.【Notes】 (1) Each individual is limited to a maximum of 5 purchases per item future sales may be suspended.(5) In case of severe weather "Visitor's Merchandise Shop"We will be selling visitor's merchandise Please check on the day for available items.【Sales Location】Merchandise Store near S16【Sales Hours】 From gate opening to 20 minutes after the end of the game ※However Notice of merchandise sales at the C Osaka match on 4/20 (Sun) SINGAPORE — With the spotlight from home firmly on him Safuwan Baharudin (picture) admitted that he was feeling a bit of pressure to live up to expectations at Melbourne City but insisted he would be ready to seize the opportunity when it arrives Singapore national defender Safuwan Baharudin has made his long-awaited debut for Melbourne City in a 3-0 defeat to city rivals Melbourne Victory today (Feb 7) who is on a three-month loan deal to the Australian Hyundai A-League side was an unused substitute during yesterday’s 2-1 win over bottom side Western Sydney Wanderers The match saw Western Sydney take a fifth minute lead through Yojiro Takahagi which are sixth in the 10-team A-League standings fought back through Jacob Melling in the 24th minute and Aaron Mooy after 73 minutes to secure the win Safuwan knows time is against him to sufficiently impress head coach John van ’t Schip to push for a permanent move “It has been difficult to handle so many things with just a few days here,” the 23-year-old “It is not easy because I have not really settled down I am happy too that I have got so much support from back home my supporters can see me play in the Melbourne shirt really soon I think I can fit in nicely (in the team).” He added: “Being the first Singaporean to play in the A-League people back home want to know how I’m doing had been hoping to get the call from Van ’t Schip during yesterday’s match at Melbourne City’s AAMI Stadium The quality of football played is totally different from the M-League or the S-League,” he said “It was a mixed feeling when I did not get any minutes (on the field) today but I will just wait and grab the opportunity when it comes I’m new and there are so many things to learn during my time at Melbourne.” Safuwan secured the loan move from Singaporean side LionsXII after impressing the club’s selectors during a 12-day training tour of the United Arab Emirates last month And his wait to make his debut for Melbourne City could happen this Saturday when the team face city rivals and two-time A-League champions Melbourne Victory at their 53,000-seater Etihad Stadium Said Safuwan: “John just told me to keep working hard when I arrived on the first day Todayonline.com and Today Online domains and apps are now part of 'Channelnewsasia.com' domain We know it's a hassle to switch browsers but we want your experience with TODAY to be fast Upgraded but still having issues? Contact us 4 MAY 2024 – As the Singapore Premier League (SPL) gears up for another thrilling season reigning league champions Albirex Niigata (S) and Singapore Cup holders Lion City Sailors are set to clash in the 16th edition of the SPL Community Shield at Jalan Besar Stadium (JBS) this Saturday The traditional curtain raiser is set to be a rematch of the 2022 SPL Community Shield that saw the Sailors ran out 2-1 winners over their Japanese opponents Both Albirex and Sailors had commendable campaigns last year with the White Swans securing their sixth SPL title and the Sailors making waves in the AFC Champions League while clinching the 2023 Singapore Cup Albirex held the upper hand in their matchups against their rivals securing victories in two out of their three encounters their paths crossed once again during the recent SPL Interim tournament in early March where Albirex emerged victorious over a youthful Lion City Sailors side Albirex has transitioned to a local outfit whereby the club will compete with a squad comprising mostly of Singaporean players Among their notable acquisitions is former Japan international Yojiro Takahagi whose wealth of experience and midfield prowess are poised to bolster Albirex’s midfield and provide a vital backbone to the team’s structure Sailors will look to their talismanic playmaker Maxime Lestienne who is the reigning SPL Player of the season to lead them to success once again The Belgian will be looking to replicate his stellar form from last season where he netted 25 goals in 24 appearances and provided 21 assists for the Sailors Yojiro Takahagi will be a key player for the White Swans Both teams are poised to unveil their latest acquisitions with the aim of enhancing their prospects for success Albirex has also signed a core of seasoned campaigners such as Ho Wai Loon Shuhei ‘Jumbo’ Hoshino and Daniel Goh in the hopes of strengthening their challenge on the league title Excitement is also brewing in the Sailors Camp as the Singapore Cup champions have added quality acquisitions for the forthcoming season Dutchman Bart Ramselar and Croatian Toni Datkovic bring with them a wealth of experience from European leagues and will be seen as the catalysts for the Sailors’ quest for success the return of midfielder Song Ui-Young adds depth to Sailors’ midfield Bart Ramselar will be a player to watch for the Sailors Kazuaki Yoshinaga’s tactical prowess propelled Albirex to their sixth title in the last campaign and positioned him as one of the league’s most successful coaches A victory on Saturday would mark his fourth SPL Community Shield win Albirex Niigata (S) Coach Kazuaki Yoshinaga Aleksandar Rankovic leads Sailors into a season brimming with challenges across multiple fronts and will be looking to begin the campaign with a piece of silverware Albirex Niigata (S): All players fit and ready to play Lion City Sailors: New signings are set to feature in the match Don’t miss the action from the SPL 2024/25 Community Shield and get your tickets via the FAS website (www.fas.org.sg/tickets) Follow us on Facebook, Twitter and Instagram Ahead of the 2024-2025 Singapore Premier League (SPL) season we look at each of the nine clubs participating in the 29th season of the domestic top flight We start with defending champions Albirex Niigata (S) Shuhei Hoshino (Japan) Stevia Egbus Mikuni (Japan) Social media platforms: Facebook | Twitter | Instagram The defending SPL champions Albirex will have their sights firmly set on a third consecutive league title heading into the 2024-2025 season The White Swans were dominant last time out securing the title with two games to spare and recording seven victories in a row to end their league campaign — with Kazuaki Yoshinaga and Seia Kunori also taking home Coach of the Year and Young Player of the Year honour at the end-of-season Football Association of Singapore (FAS) Awards Night will not be straightforward due to several changes at the club Albirex will transition to a local outfit in the upcoming season this change will see the club compete with a squad comprising mostly Singaporean players while the club may also be eligible to represent Singapore in Asian Football Confederation (AFC) tournaments They will also feel the void left by the retirement of veteran forward Tadanari Lee along with the departures of key players Kunori to BG Tampines Rovers and Riku Fukashiro to Balestier Khalsa Albirex have added ex-Japan international Yojiro Takahagi and former Japan youth international Stevia Egbus Mikuni to their ranks The Jurong-based side will also be bolstered by the return of attackers Daniel Goh and Shuhei Hoshino who netted a league-high 19 goals in 23 appearances during his previous stint at the club in 2018 Kazuaki Yoshinaga will be back in the dugout this season to provide some form of continuity The Japanese signed a new contract with the six-time SPL winners at the end of 2023 — ending speculation that he would become head coach of the Singapore national team The four-time SPL Coach of the Year will look to deliver another SPL title and guide the White Swans to a spot in the AFC Champions League 2 for the first time in club history Follow us on Facebook, X (formerly known as Twitter) and Instagram I tried using "Wi-Fi Millre" which digitizes Wi-Fi electric waves in real time and can grasp the dead space with a heat map Fast forward looking at how to create an overwhelming realistic transcendental image using Photoshop Dec 28, 2016 21:58:00 in Note Head coach Vahid Halilhodzic of Japan National Team recently announced his roster for the upcoming two matches against the United Arab Emirates (UAE) and Thailand as part of the final round of 2018 FIFA World Cup qualification later this month The 25-man squad will face the UAE on Thursday March 23 in Al Ain and Thailand on Tuesday The squad announcement sees the return of midfielders Yojiro Takahagi (FC Tokyo) and Yasuyuki Konno (Gamba Osaka) as well as the presence of familiar faces such as midfielders Makoto Hasebe (Eintracht Frankfurt) Halilhodzic said: “We are expected to play with big pressure in a unique environment We also need to stay patient without being provoked by the opponents Mental toughness and experience are important.” never lose our poise and play without fear,” added Halilhodzic teams need to finish at either one of the top two places in the 6-team group Japan have 10 points and are standing at the second place in Group B trailing Saudi Arabia who are leading only on the goal difference Goalkeepers: Shusaku Nishikawa (Urawa Red Diamonds) Midfielders: Makoto Hasebe (Eintracht Frankfurt)