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Thank you for playing DEATH NOTE Killer Within
It has been almost 6 months since the launch
To show our appreciation for your continued support since launch
we will be giving away a Headphone (Black)
an in-game item for free to all players who installed the update
Here is the latest update for DEATH NOTE Killer Within
We added two brand-new roles for even more exciting gameplay and new customization DLCs for more ways to change your appearance during investigation
The new "Watari" role knows who L is from the start
Watari can speak with L in secret using the Transceiver
passing on anything L finds on the Surveillance Cameras and
supporting L.*Unlike when Team Kira uses a Transceiver
People can figure out when Kira used the Death Note this way
forcing Team Kira to be more careful.&nbsp;Be the right-hand man for L and collaborate with him to capture Team Kira
The NPA Chief can call an Emergency Meeting and end the action phase at any time.This can only be used once per game and cannot be used with a Kira Command Card in effect
Try to use it after a Kira Command Card has ended
or when you think a meeting needs to be continued.Let the NPA Chief take the lead in meetings because all players are notified of who the NPA Chief is when the chief calls an emergency meeting
A set containing Soichiro Yagami in pajamas with a Snot Bubble and Nightcap.Idle animation changes to sprawling out and sleeping when using the Soichiro Yagami (Pajama) avatar
which allows you to obtain a variety of customization parts and rewards as you gain levels by playing
They include additional rewards if you buy the Premium Customization Track
Enjoy the thrilling game of truths and lies with two new added roles
in this latest update for DEATH NOTE Killer Within
<a href="https://en.bandainamcoent.eu/death-note/death-note-killer-within">DEATH NOTE Killer Within</a> is set for&nbsp;PC
<a class="button-base" href="https://en.bandainamcoent.eu/death-note/death-note-killer-within#shop-now">Shop now</a>
For more information about <a href="https://en.bandainamcoent.eu/death-note/death-note-killer-within">DEATH NOTE Killer Within</a> check out the <a href="/live?title=DEATH NOTE Killer Within">latest news</a>
DEATH NOTE Killer Within is an online social deduction game of life and death for up to 10 players
trying to figure out each other’s identities to either eliminate L who threatens Kira's power
© 2010 - 2025 Bandai Namco Entertainment Europe S.A.S
and its 16th and final volume in November 2023
The manga <a href="/news/2017-03-28/ms-koizumi-loves-ramen-noodles-manga-gets-tv-anime-by-studio-gokumi/.114040">inspired</a> a television anime that premiered in January 2018. <a href="/encyclopedia/company.php?id=7606">Crunchyroll</a> <a href="/news/2017-12-22/crunchyroll-to-simulcast-ms-koizumi-loves-ramen-noodles-anime/.125656">streamed</a> the series as it aired in Japan
The manga also inspired a live-action television series that premiered in 2015, followed by a New Year's Special in January 2016, a New Year's Eve Special in December 2016, and another <a href="/news/2019-03-21/live-action-ms-koizumi-loves-ramen-noodles-special-casts-yuria-kizaki/.144757">special</a> in April 2019. The live-action series' second season <a href="/news/2020-02-28/live-action-ms-koizumi-loves-ramen-noodles-gets-2nd-season-with-all-new-cast/.156931">premiered</a> in 2020
Sources: Watari-kun's ***** is About to Collapse anime's <a href="https://watarikunxx-anime.com/news/index00030000.html" target="_blank">website</a>, <a href="https://natalie.mu/comic/news/615334" target="_blank">Comic Natalie</a>
Watari-kun’s ****** Is about to Collapse (Watari-kun no xx ga Hokai Sunzen) anime revealed a teaser visual, along with the main voice cast, staff, and a July 2025 release date. Studio Staple Entertainment will be in charge of the animation, with Takashi Naoya as the director and Matsuo Asami as the assistant director. The anime <a href=https://animecorner.me/watari-kuns-is-about-to-collapse-receives-anime-adaptation/>was first announced</a> in September 2023
The voice cast is lead by Shuichiro Umeda as the titular Watari
Kodansha / Watari-kun’s ******* is About to Collapse Production Committee
L has gained two new allies in <a href="https://www.gamegrin.com/directory/game/death-note-killer-within">DEATH NOTE Killer Within</a>. Previously, there were only five roles available: L, Kira, Kira's follower, Mello, and L's investigators. But now, L has an edge, as players can now take on the role of either Watari, and the chief of the National Police Agency (NPA).
For those unfamiliar, Watari is L's trusty handler and assistant, and from the start of the game, he and L will be fully aware of who the other is. Using his transceiver, he and L can discreetly share information, which assists L's team with the investigation. Kira will need to be careful, as when Watari dies, the other players will be notified, and so Kira's identity will be in danger of being uncovered.
The second role is that of the NPA chief, Soichiro Yagami, who is also the father of Light Yagami. Playing as Soichiro, the player will be able to call an emergency meeting and end the action phase whenever, but only once per game. And it can't be done while the Kira Command Card is in play. 
Additionally, DEATH NOTE Killer Within has new DLC available, including a premium avatar of Soichiro in pyjamas with a Snot Bubble and Nightcap. This is only part of a set, which also include more in Premium Customisation Track Volume 4. Players will be able to get customisation parts and rewards as they level up, including the Watari and Soichiro avatars, and the Japanese Police Set pets, nameplates, voiced cut-ins, and various accessories.
If you're interested in playing these roles yourself, then Watari and Soichiro are available now on DEATH NOTE Killer Within, which is available on PlayStation 5, PlayStation 4, and Steam. 
Writing about all sorts like a liquorice allsort
2025Death Note: Killer Within - Official New Roles Watari &amp; NPA Chief TrailerWatch the New Roles Watari &amp; NPA Chief Trailer for Death Note: Killer Within
a strategy social deduction game developed by Grounding Inc
Players can now gain access to new roles for the game including Watari and NPA Chief to aid in the search for Kira
Watari can communicate via transceiver with L and become his right-hand man while the NPA Chief can call an emergency meeting and clinch an arrest
Watari and the NPA Chief are available now alongside a new patch for Death Note: Killer Within for PlayStation 4 (PS4)
A trailer has been released for July&#8217;s Watari-kun’s ****** Is about to Collapse anime
The short 30-second preview cycles through the main characters of the story
<a href="https://anitrendz.net/news/2025/03/12/watari-kuns-is-about-to-collapse-premieres-in-july-teaser-visual-and-more-revealed/">Watari-kun’s ****** Is about to Collapse</a> is an adaptation of the manga by&nbsp;Naru Narumi
Naoto Watari has had his share of struggles: his parents died two years ago
and now he and his sister live at his aunt’s house
his desire to protect his sister earned him notoriety as “the guy with the sister complex”—which never mattered to him
Naoto’s ordinary life is thrown into chaos … and Satsuki may just be the beginning
The 16th and final tankoubon volume was released in November 2023
• Director:&nbsp;Takashi Naoya&nbsp;(Übel Blatt,&nbsp;Tale of Wedding Rings)• Assistant director: Matsuo Asami&nbsp;(Übel Blatt) • Series composer: Tatsuya Takahashi&nbsp;(Übel Blatt)&nbsp;• Character designer: Sachiko Yasuda&nbsp;(My Happy Marriage [co-credit in Season 2])&nbsp;• Animation production: Staple Entertainment
• Shuichiro Umeda as Naoto Wataru•&nbsp;Yumika Kuno as Satsuki Tachibana•&nbsp;Yurie Igoma as Yukari Ishihara
Source: NBCUniversal Anime/Music YouTube channel
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the online social deduction game,welcomes two new roles: Watari and the NPA Chief
five roles were available in the game: L and his investigators
The two new roles are both members of L’s team which makes the game even more challenging for Kira and his team to remain undetected
Watch these two new roles in action with this trailer: 
L’s trusted handler and assistant in the anime
he and L are aware of each other’s identities
he can discreetly exchange critical information with L
giving him an edge and driving the investigation forward
Kira will have to be more careful and strategic as well because the rest of the players will be notified if Watari dies
making it easier for them to figure out who Kira is
The second role is the National Police Agency Chief
whose first and only goal is to fight for justice
the player has the ability to call for an emergency meeting and end the action phase at any time
this can only be done once per game and cannot be used while a Kira Command Card is in effect
new downloadable content is available including a set containing the premium avatar of Soichiro Yagami wearing pyjamas with a Snot Bubble and a Nightcap
players can get a variety of customisation parts and rewards as they gain levels
such as both the Watari and Soichiro Yagami avatars
About Bandai Namco Entertainment Europe S.A.S
is a leading global publisher and developer of entertainment content for platforms including
Bandai Namco Entertainment Europe is producing intellectual property for a global audience and is building its reputation with franchises such as LITTLE NIGHTMARES™
PARK BEYOND™ and UNKNOWN 9™ Awakening - developed by Reflector Entertainment based in Montreal
Bandai Namco Entertainment Europe serves as the Headquarters of Bandai Namco Holdings Inc
which is known for creating and publishing many of the Toy &amp; Entertainment industry’s top franchises
DARK SOULS™ and the recently released ELDEN RING™
For more information please visit <a href="https://www.bandainamcoent.eu/" target="_blank" rel="nofollow noopener">www.bandainamcoent.eu</a>
Press Release © 2025 Bandai Namco Entertainment Europe S.A.S
All other trademarks are properties of their respective owners
the gentle and honest man fighting using his clever set-ups and elegant techniques
joins the Guilty Gear -Strive- roster starting today as the second character of Season Pass 4
Xbox One and PC.After being left for dead during his battle with Bedman
Experience Iconic Moments and Over 30 Launch Roster Characters from the Beloved FranchiseBandai Namco Entertainment Europe today announced the launch of BLEACH Rebirth of Souls
a new action game where players fight in iconic battles based on the legendary anime franchise and experience an original story created by Tite Kubo
With the release of the fighting action game BLEACH Rebirth of Souls nearing
it is almost time for players to grip their swords and reverse their fates!In a new cinematic trailer
BLEACH Rebirth of Souls offers an immersive look into the game adapted from Tite Kubo’s legendary franchise
taking players deeper into the world of soul reapers where they will be able to impersonate iconic charact
Bandai Namco Entertainment Europe is pleased to announce SUPER ROBOT WARS Y
PlayStation 5 and PC via Steam in 2025.SUPER ROBOT WARS Y is a grid-based tactical combat RPG that brings units and pilots from a variety of anime together to battle their mutual foes
Discover some of the featured series and get ready to fight numerous enemies by watching the announcement t
Bandai Namco Entertainment Europe announces today that the iconic 90’s pocket companions are back in a new videogame with Tamagotchi Plaza,coming on 27th June 2025 for Nintendo Switch.The latest entry into the Tamagotchi Connection: Corner Shop series will bring back the unique feel of setting up quirky shops in the Tamagotchi world and serving cute characters with even more fun possibilities
Bandai Namco Entertainment Europe announced today that PATAPON 1+2 REPLAY
a remaster of the critically acclaimed PSP titles
Season 10 of MY HERO ULTRA RUMBLE starts today with a new character joining the Battle Royale; the star of General Studies
players will be able to enjoy new costumes
events and more throughout this new season.After overcoming the fear of his quirk being perceived as villainous
Shinso has gained confidence and is ready to prov
when the company took on its first clients
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The anime adaptation of the manga “ Watari-kun's XXXX is about collapse ” ( Watari-kun's ****** is about collapse ) has gained its first look
composition by Tatsuya Takahashi and character designers by Yasuda Sachiko 
Therefore the premiere of the anime “ Watari-kun's XXXX is about collapse ” ( Watari-kun's ****** is about collapse ) takes place in the July 2025 season with animation from Staple Entertainment studio
Watari Nao's life has revolved around her younger sister
He renounces club activities for the sake of spending time with her
leading many of his colleagues to label him a SISCON
Nao is willing to shut down any unwanted comments about himself
a greater danger has resurfaced – one that destroyed his way of life six years ago
and the danger comes in the form of a girl wielding a garden hoe
Watari-kun no xx ga Hōkaisunzen is a Japanese manga series written and illustrated by Naru Narumi 
It was originally serialized in Kadokawa Shoten's Young Ace magazine from August 2014 to January 2015
The series was later transferred to Kodansha's Monthly Young Magazine
where it continued from November 2015 to September 2023
Source: <a href="https://watarikunxx-anime.com/" target="_blank" rel="noreferrer noopener">Official website</a>
All images on this site belong to their respective owners
<a href="/encyclopedia/company.php?id=13564">Bandai Namco Entertainment</a> began streaming on Tuesday a video for <a href="/encyclopedia/anime.php?id=6592">DEATH NOTE</a> Killer Within, an online social deduction <a href="/news/2024-10-30/death-note-series-gets-new-online-social-deduction-game-for-ps5-ps4-pc-on-november-5/.217340">game</a> based on <a href="/encyclopedia/people.php?id=70249">Tsugumi Ohba</a> and <a href="/encyclopedia/people.php?id=5766">Takeshi Obata</a>'s Death Note manga
and it shows the new roles Watari and NPA Chief that launched on Monday:
who can communicate via transceiver with L
who can call an emergency meeting and end the action phase immediately
The game launched for <a href="/encyclopedia/company.php?id=19354">PlayStation</a> 5, PlayStation 4, and PC via <a href="/encyclopedia/company.php?id=15875">Steam</a> on November 5
up to 10 players are in one of two teams: Kira and his followers or L and the investigators
Kira's team aims to kill using the Death Note
while L's team must seize and dispose of the Death Note
Both teams are trying to uncover identities
The game features avatar customization and obtainable costumes
Mello belongs to neither team and aims to kill Kira before L's team
In Ohba and Obata's original 2003-2006 supernatural suspense manga
teenager Light Yagami finds a notebook with which he can put people to death by writing their names
He begins a self-anointed crusade against the criminals of the world
and a cat-and-mouse game begins with the authorities and one idiosyncratic genius detective
The manga inspired a 37-episode television anime series in 2006-2007
The series has also inspired live-action films, including Death Note and <a href="/encyclopedia/anime.php?id=6911">Death Note: The Last Name</a>, which premiered in Japan in 2006 and were based on the story of the manga, albeit with significant changes to the story's ending. <a href="/encyclopedia/company.php?id=10441">Netflix</a> released a live-action Death Note film by director <a href="/encyclopedia/people.php?id=162809">Adam Wingard</a> in August 2017
Watari-kun’s ****** Is about to Collapse (Watari-kun no xx ga Hokai Sunzen) anime revealed the first teaser trailer at the ongoing <a href=https://animecorner.me/tag/animejapan-2025/>AnimeJapan 2025</a> event. The anime previously revealed the <a href=https://animecorner.me/watari-kuns-is-about-to-collapse-anime-reveals-teaser-visual-main-staff-cast-july-premiere/>teaser visual, main voice cast, staff</a>
and a July 2025 release date.Studio Staple Entertainment is in charge of the animation
with Takashi Naoya as the director and Matsuo Asami as the assistant director
While the YouTube upload seems to be facing an errors
you can watch the first trailer on the anime’s official X account
Also check out our full <a href=https://animecorner.me/recap-animejapan-2025-announcements-highlights/>AnimeJapan 2025 Announcements</a> coverage
You can see the main cast below.Shuichiro Umeda as Naoto WatariYumika Yano as Satsuki TakehanaYurie Agoma as Ishihara MurasakiThe full main staff includes:
A July 2025 premiere window has been announced for the <a href="https://anitrendz.net/news/2023/09/19/watari-kuns-is-about-to-collapse-receives-anime-adaptation/?utm_source=rss&amp;utm_medium=rss&amp;utm_campaign=watari-kuns-is-about-to-collapse-receives-anime-adaptation">Watari-kun’s ****** Is about to Collapse</a> anime adaptation
Tale of Wedding Rings) is directing the Staple Entertainment production with Matsuo Asami (Übel Blatt) as assistant director
Among the other staff are Tatsuya Takahashi (Übel Blatt) as series composer and Sachiko Yasuda (My Happy Marriage [co-credit in Season 2]) as character designer
the main cast includes Shuichiro Umeda as Naoto Wataru
Watari-kun&#8217;s ****** Is about to Collapse is based on the manga by Naru Narumi
Source: <a href="https://x.com/magazine_pash/status/1899777396124918125" target="_blank">@magazine_pash</a> (via <a href="https://natalie.mu/comic/news/615334" target="_blank" rel="noopener">Comic Natalie</a>)
The official website for the <a href="/news/2023-09-19/watari-kun-is-about-to-collapse-manga-gets-anime/.202536">anime</a> of <a href="/encyclopedia/people.php?id=77749">Naru Narumi</a>'s <a href="/encyclopedia/anime.php?id=29971">Watari-kun's ***** is About to Collapse</a> (<a href="/encyclopedia/manga.php?id=22830">Watari-kun no xx ga Hōkaisunzen</a>) manga started streaming the anime's first promotional video on Sunday
The anime will <a href="/news/2025-03-12/watari-kun-is-about-to-collapse-anime-reveals-main-cast-staff-july-airing/.222238">premiere</a> in July on <a href="/encyclopedia/company.php?id=13028">Tokyo MX</a>, BS <a href="/encyclopedia/company.php?id=124">NTV</a>
Source: Watari-kun's ***** is About to Collapse anime's <a href="https://watarikunxx-anime.com/news/index00070000.html" target="_blank">website</a> via <a href="https://www.ota-suke.jp/news/283311" target="_blank">Ota-suke</a>
The dates displayed for an article provide information on when various publication milestones were reached at the journal that has published the article
activities on preceding journals at which the article was previously under consideration are not shown (for instance submission
Bioresource Technology ReportsCitation Excerpt :In addition
a DHS reactor is equipped with a sponge carrier that has a high oxygen transfer rate and does not require external aeration (Uemura et al.
High biomass retention and efficient oxygen transfer supported by sponge carriers are key to achieving high nitrification efficiency (Watari et al.
it is increasingly used in water recirculation and wastewater treatment systems in aquariums and aquaculture tanks (Adlin et al.
All content on this site: Copyright © 2025 Elsevier B.V.
<a href="https://www.flickeringmyth.com/">Flickering Myth</a>
March 26, 2025 by <a href="https://www.flickeringmyth.com/author/andrew-newton/" class="entry-author-link" itemprop="url" rel="author">Andrew Newton</a>  
Online social deduction game Death Note Killer Within,&nbsp;welcomes two new roles with the addition of Watari and NPA Chief
only five roles were available &#8211; Kira and Mello
and L and his investigators.&nbsp; These two new roles both belong on L&#8217;s team
making the task of being undetected all the more challenging for Kira and Mello.&nbsp; Watch the new trailer below to see these new roles in action
Watari is L’s trusted handler and assistant and will play a crucial role in the game from the start as he can discreetly exchange information with L via a transceiver.&nbsp; This will provide L an edge in his investigation as should Watari die
The second new addition is National Police Agency (NPA) Chief Soichiro Yagami
players will have the ability to call for an emergency meeting and end the action phase at any time they wish
this can only be performed once per game and not when a Kira Command Card is in effect
new downloadable content is available that includes a set featuring a premium avatar of Soichiro Yagami wearing pyjamas with a Snot Bubble and a Nightcap.&nbsp; There&#8217;s the Premium Customisation Track Vol
where players can get a variety of customisation parts and rewards
including the Watari and Soichiro Yagami avatars
the &#8220;Japanese Police Set&#8221; pets
Log into Death Note Killer Within&nbsp;now and try these new roles
 <a href="https://animenew.com.br/en/news/anime/">anime</a> adaptation of the manga “ Watari-kun's XXXX is about collapse ” ( Watari-kun's ****** is about collapse ) won his first trailer
Therefore the premiere of the anime “ Watari-kun's XXXX is about collapse ” ( Watari-kun's ****** is about collapse ) takes place in the July 2025 season with animation from Staple Entertainment  
Makoto Takatsu’s Watari Tori to Katatsumuri (Migratory Birds & Snail) manga is returning from hiatus after almost three years and it coming back big time with an anime adaptation that is in the works. The manga went on hiatus after the release of the third volume in 2021. The <a href=https://wanibooks-newscrunch.com/articles/-/5340>new chapter</a> (19th) is now out
The manga was initially serialized in the Wani Books’ Comic Gum web magazine from 2019 to 2021
It will be returning in Wani Books’ News Crunch online magazine
The volumes were published under the Crunch Comics imprint
a young man who works at a machine parts wholesaler
a picture book writer who meets him by chance
who was driving his car after failing at work and wanting to go somewhere far away
suddenly stops at a roadside station and is approached by Tsugumi
a beautiful girl of unknown age traveling with her pet dog
The car where she uses is like a secret base…Takatsu issued a statement:The migratory birds and snails began their journey in the summer of 2019.While visiting roadside stations and car camping spots around the country for interviews
and Umemayo were traveling in my daily life
I am excited to seethem sleeping in their cars in the crimson-hued dusk or the forest at dawn
where the breathing of the trees drifts like a cloud
An announcement illustration for the Watari Tori to Katatsumuri anime is now available:<a href=https://static.animecorner.me/2024/07/1720603681-594e0e0e454dee696ce165e51cf633b9.png data-rel=penci-gallery-image-content></a>Source: <a href=https://x.com/Crunch_comics82/status/1810962414260625531>Official Twitter/X</a>, <a href=https://wanibooks-newscrunch.com/articles/-/5340>Official Website</a>© Makoto Takatsu
While performing a ritual for the God of Wealth on February 7
Vietnam was unexpectedly visited by Cyro Watari
a 59-year-old Brazilian traveler who had come all the way to meet him after being astonished by their uncanny resemblance.&nbsp;
Viet told Tuoi Tre (Youth) newspaper that Watari expressed his surprise when he saw Viet
who looked so much like him and had come all the way to meet him in person
Watari was able to find Viet because Viet is an artist
as another surprise followed when Watari showed Viet photos that merged their photos
One half was a photo of Viet posted on his personal Facebook page
All of them were downloaded and merged by Watari himself.&nbsp;
It was not&nbsp;until they met that the photos were revealed
Viet admitted that they had many things in common
They share passions of travel and exploration
&ldquo;It&#39;s great to have an international friend
The fact that we look so alike makes this friend even more special,&rdquo; Viet shared
The two-day meeting between Watari and Viet in Kon Tum was full of surprises
There seemed to be no awkwardness between them.&nbsp;
Using smartphones and automatic translation software
Watari said there is&nbsp;research that suggests every person in the world has one or more people who look like them
Viet told&nbsp;Tuoi Tre that he was happy that Watari was knowledgeable about cultures in other countries and regions around the world.&nbsp;
Viet also took the opportunity to share information about Vietnamese culture and people in the Central Highlands.&nbsp;
Watari left Kon Tum and resumed his travels throughout Vietnam
<a href="https://www.facebook.com/newsaboutvietnam/" target="_blank">Like us on Facebook</a>&nbsp;or&nbsp;<a href=" https://twitter.com/i/user/262586685" target="_blank"> follow us on Twitter</a>&nbsp;to get the latest news about Vietnam
Your comment will be reviewed before being posted
Bandai Namco added two new playable papers to the <a href="https://www.bandainamcoent.com/games/death-note-killer-within">Death Note: Killer Within.</a> Watari and Soichiro Yagami
They will have strategic functions that expand the range of actions within the game
which also includes new cosmetic content and paid features for customization
With a visual style faithful to the original work
Death Note: Killer Within is an online social deduction game with support for up to ten players in matches that mix logic
The new update reinforces the game's proposal by introducing mechanics that refer directly to the anime narrative
has the ability to communicate secretly with L through a transmitter radio
allowing the exchange of information that favor the advance of investigations
It can stop the round with an emergency meeting and arrest suspects
but players can only use this action once per match
the update has brought additional items for those seeking customization
Among the highlights is the premium Avatar of Soichiro Yagami
with a comic version of the character in pajamas
Bandai Namco has launched the Premium Custom Trail Vol
identification plates and exclusive voice lanes
The new additions not only renew the interest of players
but also expand the strategic depth of matches
The functions of Watari and NPA Chief are part of the L team and present unique approaches to confront Kira's followers
Soichiro's mechanics prevent the activation of the function when Kira's command card is at stake
which forces players to choose the right time to act
Launched exclusively for PC and PlayStation 4 and 5 consoles
Death Note: Killer Within is the first official online game based on the anime universe
The proposal is to turn the tension climate of the original story into an interactive experience
where each player takes a secret role with different goals
In addition to the classic papers like Kira
the title now has the additions of Watari and the Chief of Police
totaling seven papers with their own characteristics
they must reveal - or hide - their true identities
plan actions and avoid falling into Kira's traps
the experience remains accessible and integrated among available platforms
The arrival of new content shows that Bandai Namco intends to keep the title constantly evolving
updating both the visual aspect and the game dynamics
Tokyo Art Beat presents a selection of the best exhibitions opening in December 2024. Bookmark the exhibitions on the <a href="https://www.tokyoartbeat.com/en/mypage/sign-in" target="_blank" rel="noreferrer">TAB website</a> or <a href="https://www.tokyoartbeat.com/en/aboutApp" target="_blank" rel="noreferrer">TAB app</a> and never miss the openings and closings
Artist Mana Konishi is widening the possibilities of landscape painting
which capture magnificent landscapes on large canvases and are meticulously painted down to the smallest detail
When she was forced to live in isolation due to the pandemic
Konishi visited the municipal park within walking distance of her home
and the stream that flows nearby and continued to paint these landscapes
This exhibition will be Konishi&#x27;s first large-scale solo exhibition at an art museum
and in addition to her previous representative works
Venue: <a href="https://www.tokyoartbeat.com/en/venues/-/DE1022E7" target="_blank" rel="noreferrer">Fuchu Art Museum</a>Schedule: December 14 - February 24Fee: Adults ¥800
Junior High and Elementary School Students ¥200
people have wished for a happy life and entrusted their hopes to patterns and shapes
Japanese art is full of symbols of good fortune that are displayed at celebrations such as births and weddings
This exhibition focuses on art that expresses wishes for long life
and includes many familiar motifs such as pine
as well as other works that can be said to be lucky motifs for people today
Venue: <a href="https://www.tokyoartbeat.com/en/venues/-/6A8A7FB7" target="_blank" rel="noreferrer">Yamatane Museum of Art</a>Schedule: December 14 - February 24Fee: Adults ¥1400
Junior High School Students and Under free
Ryuichi Sakamoto continuously paved the way for his era through diverse and cutting-edge artistic activities
he devoted himself to creating three-dimensional sound installations in exhibition spaces
which he developed and realized in collaboration with various artists
Focusing entirely on large-scale installation works
this first comprehensive exhibition in Japan looks back on Sakamoto&#x27;s pioneering
including some of his most well-known previous pieces and new works that he envisioned for this particular occasion before his passing
which will be dynamically constructed in and around the museum building
Venue: <a href="https://www.tokyoartbeat.com/en/venues/-/4A4AABB8" target="_blank" rel="noreferrer">Museum of Contemporary Art Tokyo</a>Schedule: December 21 - March 30Fee: Adults ¥2400; University Students
Seniors 65 &amp; Over ¥1700; High School and Junior High School Students ¥960; Elementary School Students and Under free
This exhibition provides an overview of artist Yosuke Amemiya&#x27;s practice from the beginning of his career
From the works of the early 2000sーthe melted apple sculptures
the document video of Ishinomaki Thirteen Minutes
which will be filmed at the museum during the installation for this exhibitionーhis works provide new art experiences
Amemiya will hold an open practice for his final work every Saturday evening
Venue: <a href="https://www.tokyoartbeat.com/en/venues/-/7879E100" target="_blank" rel="noreferrer">Watari-um, The Watari Museum of Contemporary Art</a>Schedule: December 21 - March 30Fee: Adults ¥1500; University Students (Under 25)
Persons with Disability Certificates + 1 Companion ¥1300; Junior High and Elementary School Students ¥500
examples of old calligraphy are generally called kohitsu
&quot;ancient handwriting.&quot; In a narrow sense
that term refers to Japanese calligraphy in poetry collections from the Heian through the Kamakura periods
aristocrats commissioned talented calligraphers to copy poetry anthologies or their private collections as gifts or furnishings
single sheets or pages or even a few lines were extracted from those works for the increasingly popular tea gatherings and personal appreciation
Those segments detached from ancient calligraphic works are known as kohitsugire
&quot;detached segments of ancient Japanese calligraphy.&quot; This exhibition showcases examples of these detached segments of ancient calligraphy from the Heian through the Kamakura periods
Venue: <a href="https://www.tokyoartbeat.com/en/venues/-/53ACE7A1" target="_blank" rel="noreferrer">The Nezu Museum</a>Schedule: December 21 - February 9Fee: [Online ticket] Adults ¥1300
Due to the diversification and personalization of contemporary society
color has become an increasingly important role not only in fashion and interior design but also in fields such as the environment
as smartphones now can reproduce over one billion colors
we have become unknowingly engulfed in a vast world of color
Focusing on color in art from the modern to the contemporary era
this exhibition aims to reconsider the role of color while touching on subjects such as the relationship between color theory and the materials used to express color
Venue: <a href="https://www.tokyoartbeat.com/en/venues/-/B4BF1F55" target="_blank" rel="noreferrer">Pola Museum of Art</a>Schedule: December 14 - May 18Fee: Adults ¥2200
Persons with Disability Certificates + 1 Companion ¥1100
Takashi Kuribayashi lives between Indonesia and Japan
he has consistently presented works in Japan and abroad that urge viewers to have physical experiences through various media
under the theme of &quot;boundaries.&quot; Created from the artist&#x27;s perspective
this latest solo exhibition uses various spaces outside the exhibition room
On view are installations conceived for spaces not initially proposed for exhibition
as well as previously unpublished drawings and video works
to show the &quot;boundary = now&quot; between the past and the future of the artist who has been expanding his areas of activity in recent years
Venue: <a href="https://www.tokyoartbeat.com/en/venues/-/E06DA77D" target="_blank" rel="noreferrer">Museum of Modern Art, Hayama</a>Schedule: December 14 - March 2Fee: Adults ¥1000; Under 20s and University Students ¥850
Seniors 65 &amp; Over ¥500; High School Students ¥100; Junior High School Students and Under
Persons with Disability Certificates + 1 Companion free
one of Japan&#x27;s preeminent wood and lacquer artisans
was the first person to be designated as the holder of an important intangible cultural property (Living National Treasure) in woodworking
Kuroda is also known for questioning the then-standard practice of labor division in lacquer-making
doing everything from creating the design to performing the groundwork and applying the decorations
Kuroda pioneered a unique creative world based on a union of practicality
Marking the 120th anniversary of Kuroda&#x27;s birth
this exhibition presents the life of this artist who made a significant mark on the history of Japanese crafts through a collection of works spanning his career
Venue: <a href="https://www.tokyoartbeat.com/en/venues/-/CF834846" target="_blank" rel="noreferrer">The National Museum of Modern Art, Kyoto</a>Schedule: December 17 - March 2Fee: Adults ¥1200; University Students ¥500; High School Students and Under
This exhibition showcases the activities and work of the husband-and-wife couple of Josef Müller-Brockmann
The exhibition commemorates the 160th anniversary of establishing diplomatic relations between Switzerland and Japan and is the first large-scale exhibition to feature either Yoshikawa or Müller-Brockmann in Japan
Venue: <a href="https://www.tokyoartbeat.com/en/venues/-/nakanoshima-museum-of-art-osaka" target="_blank" rel="noreferrer">Nakanoshima Museum of Art, Osaka</a>Schedule: December 21 - March 2Fee: Adults ¥1700
the Great Hanshin-Awaji Earthquake caused extensive damage to the predecessor of the Hyogo Prefectural Museum of Art
both in terms of the building and the collection
The Hyogo Prefectural Museum of Art opened in 2002 as a cultural symbol of post-earthquake reconstruction and has held exhibitions related to the earthquake
this is the first independently planned exhibition in the museum&#x27;s history
This exhibition will be an opportunity to reexamine the theme of hope from a variety of perspectives and through a variety of expressions
Venue: <a href="https://www.tokyoartbeat.com/en/venues/-/BBE1BC75" target="_blank" rel="noreferrer">Hyogo Prefectural Museum of Art</a>Schedule: December 21 - March 9Fee: Adults ¥1600
Persons with Disability Certificates (Adults) ¥400
Persons with Disability Certificates (University Students) ¥250
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which exhibits superb works of contemporary art from its own collection
the museum aims to provide opportunities for people to experience contemporary art and to educate the next generation of art curators
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By Mutsumi Morita / Yomiuri Shimbun Staff Writer
Works on display include paintings by German contemporary master Gerhard Richter and Japanese artist Takashi Murakami
whose superflat movement is sweeping the world
Other featured artists include Olafur Eliasson
The exhibits represent a stunning volume of overwhelmingly excellent work by all-star artists currently active in Japan and abroad
“I want to share art with people around the world
I’ve been showing my collection on my website and social media
I also wanted to create a place for people to see the art for real,” said Kankuro Ueshima
the entrepreneur and investor who established the museum
With the theme of “contemporaneity,” the museum will exhibit selected pieces from the more than 650 works that Ueshima has amassed in about 2 years of serious collecting
His attitude is an unusual one among private collectors
who often do not open their collections to the public
a building in the Shibuya Kyoiku Gakuen school complex near the Miyashita Park commercial facility
The building has one basement floor and six floors above ground
with a total floor area of about 1,400 square meters
which stretches from the basement to the fifth floor
It is planned for some of the exhibits to be changed every six to 10 months
are expected to be put on display in the future
The museum will also serve educational purposes by hosting interactive art viewing events and providing opportunities for students and young curators to plan exhibitions
said: “I hope the museum will not just be a place where people can see works of art
but also will somehow have an effect on society through its exhibitions.”
Ueshima said: “I think it will be very important to keep the museum running long-term
I want to have everyone look at the art and stimulate me to enrich the collection.”
Visitors are required to make reservations in advance
Private museums of contemporary art with unique collections have made their presence felt in the art world
also known as The Watari Museum of Contemporary Art
The museum was established in 1990 by Shizuko Watari (1932-2012)
with pieces that she had collected in the 1980s
The museum has organized and hosted solo and group exhibitions of contemporary artists and architects
including German artist Joseph Beuys (1921-1986) and architect Arata Isozaki
The museum introduces visitors to some of the most cutting-edge art in Japan and abroad
greatly contributing to broadening the fan base of contemporary art
titled “Umetsu Yoichi: Exhibition Maker,” and organized by artist Yoichi Umetsu
The core works featured are from Watari’s collection
including some by Tetsuro Komai and Natsuyuki Nakanishi
and they are supplemented by works by Umetsu himself
Watari’s eldest daughter and the museum’s director
said: “The exhibition is an unexpected combination of works of art
I was able to discover new ways to appreciate the items in our collection.”
The exhibition is closed on Mondays except July 15
Our weekly ePaper presents the most noteworthy recent topics in an exciting
&#169; 2025 The Japan News - by The Yomiuri Shimbun
Anime Corner had the wonderful opportunity to interview Wataru Watari
the author of My Youth Romantic Comedy Is Wrong
Watari’s works include Girlish Number and Qualidea of Scum And a Gold Coin (co-written by Sou Sagara)
Hoshino is currently the chief editor at Gagaga Bunko
He joined the light novel editing department in 2008 and even assisted with the My Teen Romantic Comedy anime series
it’s an honor to be able to interview both of you today
Thank you for taking the time to speak with me
Watari-san has previously mentioned that he originally had no intention of writing a romantic comedy until he saw the demand for it
What was it like between the two of you when discussing the story for My Youth Romantic Comedy before it even started
And what were your personal thoughts on the idea behind the story
Hoshino: Watari-san’s debut story was about a samurai
So then we decided that for the next story
we wanted something that would cater to the demand
But I didn’t really know if Watari-san would be able to really do that
But seeing a lot of his SNS [social networking service] and social media posts
I saw that they were really funny and somewhat cynical
So I thought he would be the one to make this happen.Q: Contrast is a big part of any form of storytelling
When we take a look at the main group in My Youth Romantic Comedy
Hachiman and Yukino are similar upfront but had different bases
Yui is exceptionally different on many levels
Was making them contrasting characters an idea you had going into the story or were their little
individual differences something that just came along as you wrote the story
that was the attempt before writing the story
Yukino and Hachiman are somewhat similar characters but at the same time
The motivation behind doing that is that we wanted to make some sort of paradox and illustrated the paradoxical nature of social interactions
So they’re similar but in some ways the most different
Maybe some people who are the most different can end up being
more similar as they interact more.Q: Hoshino-san
were there any specific ideas that you had for the series that were eventually implemented in the final product that many fans might not know about
But Watari-san ends up adding a lot of his own interpretations to the given ideas
So it ends up being something that’s unique to him and sort of different from my original ideas
So looking back to my ideas and what he ended up with I sort of can’t recognize any of my original ideas in the story
you said in a previous interview that while writing Hachiman you even wondered yourself why he is so skeptical
Were there other times like this when you were questioning the characters you were writing
Watari: Almost all of the characters actually
(laughs) So I often think with certain characters
“Why are you doing that?” or “You should speak up
So I really wanted to illustrate the drama among the characters by intentionally adding questionable aspects of the characters.Q: You’ve mentioned before how much fun Iroha was to write
Could you explain a little in detail about why that is for the fans
especially the ones that shipped her with Hachiman over Yukino and Yui
Iroha is a very unique character and I refer to her as the world’s cutest annoying girl (laughs)
So I really enjoy writing her!Q: Yui can be seen as the most troubled character with a lot of character development
possibly more so than others from the story
Is her growth while being troubled a primary reason that her own side story started being published
Or was there simply a big demand from fans
Watari: There wasn’t really a fan demand [for the Yui spin-off]
We really didn’t take that into consideration
(laughs)Hoshino: So as we were writing the story
there were a lot of different possibilities to the story that branches off
we thought that there could be another story going on at the same time.Q: It’s unfortunate that we’re short on time because I have a million questions I would love to ask both of you
How would you describe this incredible journey with My Youth Romantic Comedy
Photo credits: Brian Howard (<a href=https://twitter.com/FoxyGrandpa target=_blank rel="noreferrer noopener">Twitter</a>)Translator: Seiya Furukawa (Otakon); Assistance: Jeremy Sauer
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While the short-term relationship between clouds and aerosols is well known
no adequate data is available to verify the longer-term
It is important to quantify the aerosol–cloud interaction (ACI) for mitigating uncertainty in climate prediction
Here the long-term ACI over the mid-to-high latitudes of the Northern Hemisphere was analyzed by using seasonally-resolved ion fluxes reconstructed from a southeastern Greenland ice core (SE-Dome ice core) as aerosol proxies
and satellite-based summer cloud amount between 1982 and 2014
SO42− flux in the ice core shows significant positive correlation with total cloud amounts (\({CC}_{T}\)) and cloud droplet concentration (\({N}_{d}\)) in the summer over the southeastern Greenland Sea
implying that the sulfate aerosols may contribute to the variability of \({CC}_{T}\) via microphysical cloud processes
Significant positive correlations are persistent even under the constrained conditions when cloud formation factors such as relative humidity
and summer North Atlantic Oscillation are limited within ± 1σ variability
Hence sulfate aerosols should control the interannual variability of summer \({CC}_{T}\) In terms of decadal changes
\({CC}_{T}\) was approximately 3–5% higher in the 1960s–1970s than in the 1990s–2000s
\({{{\text{SO}}}_{4}}^{2-}\) flux preserved in the SE-Dome ice core
thus the use of reanalysis data is not optimal for a detailed comparison between clouds and aerosols
combined with some passive cloud datasets to achieve increased temporal resolution with high spatial resolution covering the whole globe
(b) The area-averaged correlation coefficients between aerosol proxies preserved in the SE-Dome ice core and cloud and atmospheric properties in the target domain
The vertical axis shows the cloud properties (red text); total cloud amounts (\({CC}_{T}\))
and cloud droplet concentration (\({N}_{d}\))
and the atmospheric properties (black text); wind components at 10 m height (\({U}_{10m}\) and \({V}_{10m}\))
downward solar radiation at the surface (\({R}_{sd}\))
The horizontal axis shows the aerosol proxies reconstructed in the SE-Dome ice core
White asterisks in panel b denote p &lt; 0.05
\({{{\text{SO}}}_{4}}^{2-}\) flux in the SE-Dome ice core is highly correlated with cloud amounts which varies with the COD
suggesting that summer SO42− flux contributes to marine cloud formation over the domain
</a>\({N}_{d}\) have positive correlation with SO42− flux and \({CC}_{T}\) in the target domain
(a) Geographical distribution of correlation coefficients between SO42− flux and \({N}_{d}\) for low-level warm clouds with a resolution of 0.5º from 1982 to 2014
(b) Dependence of \({CC}_{T}\) on \({N}_{d}\) in the target domain (black polygon in panel a)
Error bars represent 1 standard deviation of the monthly \({CC}_{T}\)
</a>Relationships between \({CC}_{T}\) and SO42− flux under the constrained atmospheric conditions for cloud formation
Geographical distributions of the correlation coefficients between SO42− flux and \({CC}_{T}\) of (a) CCI and (b) ERA5 datasets under the constrained atmospheric conditions (\({T}_{cloud\_height}\)
and sNAO within &lt;  ± 1σ) in the target domain
The mean \({CC}_{T}\) and \({N}_{d}\) in the target domain from 1982 to 2014 were 82.7 ± 7.8% and 94.0 ± 14.7 cm−3
while the \({CC}_{T}\) and \({N}_{d}\) anomalies were observed to be up to 8.9% and 59.1 cm−3
The observed changes in \({CC}_{T}\) and \({N}_{d}\) can be attributed to the increased \({{{\text{SO}}}_{4}}^{2-}\) flux at the SE-Dome site
The atmospheric transport processes from the emission sources to the SE-Dome site should have accounted for the higher \({{{\text{SO}}}_{4}}^{2-}\) fluxes
the higher \({{{\text{SO}}}_{4}}^{2-}\) could have increased the cloud amount
The relationship between 11 year-averaged \({{{\text{SO}}}_{4}}^{2-}\) flux (\({F}_{{\text{SO}}4}\)) and satellite-based \({CC}_{T}\) (%) are obtained for the 33 year period from 1982 to 2014 (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s41598-024-59817-7#Fig4">4</a>a) to reconstruct past cloud amounts:
</a>Reconstruction of CCT from the SO42− flux in ice-core
(a) The scatter plot of the \({CC}_{T}\) and SO42− flux from 1982 to 2014 with a quadratic approximation (black line)
The dashed red line indicates the value of 15.28 mg m−2 season−1 in SO42− flux
(b) An 11 year running mean of reconstructed \({CC}_{T}\) from the SE-Dome ice core (blue line) and satellite-based \({CC}_{T}\) in the CCI dataset averaged for the target domain (red line)
the absence of a clear correlation for small values of \({{{\text{SO}}}_{4}}^{2-}\) flux in the relationship between averaged \({{{\text{SO}}}_{4}}^{2-}\) flux and satellite-based \({CC}_{T}\) for 55 years suggests a threshold of 15.28 mg m−2 season−1 due to the highest correlation to increases cloud amount effectively
This implies that an increase in aerosols does not directly cause an increase in amount of cloud
but that a certain amount of aerosols is required for a substantial increase in cloud amount
The concept of a threshold may be important in reconstructing cloud amounts over a wide area
which is required for further comparison between ice core data and satellite (or reanalysis) measurements
and would lead to improvement of physical mechanisms in aerosol-climate models
pointed out in the review that the degree to which human activities affect natural aerosol levels
to aerosol perturbations remain particularly uncertain
The present paper reconstructs \({CC}_{T}\) during 1960–70 in Greenland
when the \({{\text{SO}}}_{x}\) emission maximum from around countries
The relationship between \({{{\text{SO}}}_{4}}^{2-}\)- aerosols and \({N}_{d}\) (and \({CC}_{T}\)) during the \({{\text{SO}}}_{{\text{x}}}\) emission maximum will help to reduce the uncertainty in projections of future aerosols interacting with radiation and clouds through an improvement of climate models
The black line shows the area where the integrated probability of air mass at the SE-Dome was more than 50%
If the correlation coefficient between \({{{\text{SO}}}_{4}}^{2-}\) flux and cloud amount is still significant even under the constrained conditions of cloud formation factors (14-period)
the variability of cloud amounts can be explained by the variability of aerosol concentrations
aerosol fluxes are obtained using the accumulation rate of the SE-Dome ice core multiplied by the ion concentration
The CCI datasets from 1982 to 2014 were used in this research
providing sufficient samples to obtain statistically significant results of long-term ACI
The advantage of the CC4CL algorithm is that the visible infrared imager (AVHRR) has a proven track record of long-term observations and is useful for climate research
the disadvantage is that it is a passive sensor
so information on the vertical structure of clouds is limited
and so estimation of overlapping clouds is subject to large errors
and the condensation rate (\({C}_{w}\)) is calculated using parcel model with ERA5 datasets as follows
The adiabatic liquid water content at different cloud base temperatures for a given 900 hPa is calculated using ERA5 data
The altitude at 900 hPa is defined as the cloud base pressure in this research
the dependence of \({C}_{w}\) on the cloud base temperature (\({T}_{c}\)) was obtained in order to perform a regression analysis between calculated water condensation and liquid water content
The maximum and minimum temperatures in the target domain are 265.8 (K) and 298.7 (K)
thus the relationships within this temperature range are investigated
shows the relative uncertainty associated with cloud optical thickness and cloud particle size are both less than 20%
\({N}_{d}\) is proportional to 1/2 power of cloud optical thickness and − 5/2 power of cloud particle size (assuming that the uncertainties of these other variables are sufficiently small)
so the relative uncertainty of \({N}_{d}\) is calculated as less than 60% (\(&lt;\frac{1}{2}\left(20\%\right)+\frac{5}{2}(20\%)\)) )
Code for calculations and data processing are available from the corresponding author upon request
Aerosol effects on cloud cover as evidenced by ground-based and space-based observations at five rural sites in the United States
Significant underestimation of radiative forcing by aerosol–cloud interactions derived from satellite- based methods
Indirect and semi-direct aerosol campaign: The impact of arctic aerosols on clouds
Lightning enhancement over major oceanic shipping lanes
Bellouin, N. et al. Bounding global aerosol radiative forcing of climate change. Rev. Geophys. <a href="https://doi.org/10.1029/2019RG000660" data-track="click_references" data-track-action="external reference" data-track-value="external reference" data-track-label="10.1029/2019RG000660">https://doi.org/10.1029/2019RG000660</a> (2020)
Analyzing the uncertainties between reanalysis meteorological data and ground measured meteorological data
Intercomparison of Arctic regional climate models: Modeling clouds and radiation for SHEBA in May 1998
Processes and impacts of Arctic amplification: A research synthesis
High-resolution aerosol concentration data from the Greenland North GRIP and NEEM deep ice cores
East Greenland ice core dust record reveals timing of Greenland ice sheet advance and retreat
Seasonal-scale dating of a shallow ice core from Greenland using oxygen isotope matching between data and simulation
A 60 year record of atmospheric aerosol depositions preserved in a high-accumulation dome ice core
Analyzing the arctic feedback mechanism between sea ice and low-level clouds using 34 years of satellite observations
Cloud_cci advanced very high resolution radiometer post meridiem (AVHRR-PM) datasets version 3: 35 Year climatology of global cloud and radiation properties
The Community Cloud retrieval for CLimate (CC4CL)-part 1: A framework applied to multiple satellite imaging sensors
The Community Cloud retrieval for CLimate (CC4CL)-part 2: The optimal estimation approach
Forty years of improvements in European air quality: Regional policy-industry interactions with global impacts
Dominant role of mineral dust in cirrus cloud formation revealed by global-scale measurements
Annual distributions and sources of Arctic aerosol components
Sulphuric acid closure and contribution to nucleation mode particle growth
Aerosol–cloud interactions in ship tracks using Terra MODIS/MISR
Large contribution of organic aerosols to cloud-condensation-nuclei concentration
Under what conditions can we trust retrieved cloud drop concentrations in broken marine stratocumulus?
Correlation between cloud condensation nuclei concentration and aerosol optical thickness in remote and polluted regions
Aerosol-driven droplet concentrations dominate coverage and water of oceanic low-level clouds
Synoptic variability of low-cloud properties and meteorological parameters in the subtropical trade wind boundary layer
Numerical simulations and a conceptual model of the stratocumulus to trade cumulus transition
High flux of small sulfate aerosols during the 1970s reconstructed from the SE-Dome ice core in Greenland
The summer North Atlantic oscillation: Past
Ice core drilling and related observations at SE-Dome site
SE-Dome II ice core dating with half-year precision: Increasing melting events from 1799 to 2020 in southeastern Greenland
Noaa’s hysplit atmospheric transport and dispersion modeling system
Increasing dust emission from ice free terrain in southeastern Greenland since 2000
Pinsky, M. et al. Analytical estimation of droplet concentration at cloud base. J. Geophys. Res. <a href="https://doi.org/10.1029/2012JD017753" data-track="click_references" data-track-action="external reference" data-track-value="external reference" data-track-label="10.1029/2012JD017753">https://doi.org/10.1029/2012JD017753</a> (2012)
Variability of optical depth and effective radius in marine stratocumulus clouds
Long-term cloud condensation nuclei number concentration
particle number size distribution and chemical composition measurements at regionally representative observatories
<a data-track="click" data-track-action="download citation references" data-track-label="link" rel="nofollow" href="https://citation-needed.springer.com/v2/references/10.1038/s41598-024-59817-7?format=refman&amp;flavour=references">Download references</a>
We are grateful to the drilling and initial analysis teams of the SE-Dome ice core project
This work was supported by JSPS KAKENHI Grant Numbers 18H05292 and 23H00511
and the Joint Research Program and Readership program of the Institute of Low Temperature Science
This study was a part of ArCS II (Arctic challenge for sustainability II
This work was supported by Japan Society for the Promotion of Science
Arctic Challenge for Sustainability II Program
Present address: Nippon Koei Energy Solutions Co.
Institute for Space-Earth Environmental Research
Graduate School of Fisheries and Environmental Sciences
All authors contributed to revising the paper
The authors declare no competing interests
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations
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DOI: https://doi.org/10.1038/s41598-024-59817-7
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Veteran Japanese action movie star and singer Tetsuya Watari
known for his role in the hit police drama "Seibu Keisatsu" (Western Police)
died of pneumonia at a hospital in Tokyo earlier this week
He had remained active in show business even after undergoing an operation for rectal cancer in 1991
Watari gained popularity on television for his roles in police drama series that began in the 1970s
such as "Dai Tokai" (The Big City) and "Seibu Keisatsu." He was also praised for his roles in movies about yakuza gangsters such as "Tokyo Drifter" and "Graveyard of Honor."
including the smash hit "Kuchinashi no Hana" (gardenia flower)
The Hyogo Prefecture native was scouted while attending Aoyama Gakuin University in Tokyo
and made his starring debut in 1965 in the film "Abare Kishido" (Wild Chivalry)
He appeared in many productions by major filmmaking company Nikkatsu
before joining a production company founded by fellow action star Yujiro Ishihara
The veteran actor was part of the "Ishihara Gundan," meaning Ishihara Corps
in reference to actors who belong to Ishihara's talent agency
will end its talent agency operations early next year
Watari also devoted himself to volunteer activities following major disasters in Japan
About a month after a powerful earthquake and tsunami devastated northeastern Japan on March 11
he visited the tsunami-hit city of Ishinomaki in Miyagi Prefecture and was involved in soup kitchens for displaced local residents
A 77-year-old woman who recalled receiving food from him at the time said of the actor's death
helped to prepare food with Watari in the aftermath of the disaster
Noting that Watari told her nobody can beat him when it comes to cooking yakisoba stir-fried noodles
particularly showing his smile to children."
Watari also visited his native Hyogo Prefecture to cheer up local residents about a month after a strong quake rattled its port city of Kobe and the vicinity on Jan
Watari brought food by truck to neighboring Ashiya City where they cooked yakisoba and other dishes for locals for several days
remembers interacting with Watari at the time
"In contrast to his terrifying image in police dramas
died in March 2017 of multiple organ failure at the age of 72
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Decarbonization strategies for the cement and concrete sector have relied heavily on supply-side technologies
including carbon capture and storage (CCS)
masking opportunities for demand-side intervention
Here we show that cross-cutting strategies involving both the supply and demand sides can achieve net-zero emissions by 2050 across the entire Japanese cement and concrete cycle without resorting to mass deployment of CCS
Our analysis shows that a series of mitigation efforts on the supply side can reduce 2050 CO2 emissions by up to 80% from baseline levels and that the remaining 20% mitigation gap can be fully bridged by the efficient use of cement and concrete in the built environment
this decarbonization pathway is dependent on how CO2 uptake by carbonation and carbon capture and utilization is accounted for in the inventory
Our analysis underscores the importance of including demand-side interventions at the heart of decarbonization strategies and highlights the urgent need to discuss how to account for CO2 uptake in national inventories under the Paris Agreement
the key question centers on what actions need to be taken
in order to decarbonize the complete cement and concrete cycle
they tend to consider only a limited set of concrete end uses and thus do not capture a large part of concrete flows and stocks
These trends provide a solid foundation for exploring how the cement and concrete cycle may evolve in the future
</a>Conventional supply-side strategies are envisioned to enhance the current cement and concrete industry’s efforts to reduce CO2 emissions
while emerging supply-side strategies refer to strategies that are innovative
Demand-side strategies refer to interventions by systems and actors that use concrete in the built environment
Since most of the CO2 emissions associated with the cement and concrete cycle are dominated by limestone calcination and fuel combustion for heat supply
the emission saving from electricity decarbonization is limited to 8% in 2050
reflecting the fact that the thermal and electrical energy efficiency of Japanese cement facilities is already close to the global 10% best-in-class
energy efficiency improvements would mitigate emissions by only 3% in 2050
what would truly be effective is fuel switching
Switching from the coal used in cement kilns to low-carbon fuels and reducing the clinker-to-cement ratio by increasing the use of SCMs could provide additional mitigations of 11% and 25% in 2050
systemic improvements in operations and logistics across all aspects of road freight
and support for the use of alternative fuels in transport could result in additional savings of 10% in 2050
Further mitigation could be achieved by several emerging strategies that are currently under active development in the cement and concrete industry
We estimate that additional emissions reductions of 13% and 7% could be achieved by 2050 through maximizing the use of low-carbon cement chemistries and CCU technologies (both concrete curing and mineralization)
even if all of these measures are implemented
the collective mitigation potential is only ~80%
which is 20% short of achieving net-zero emissions
This reflects the fact that emerging supply-side strategies are limited in their potential by the applicable concrete strength class and the availability of industrial waste
suggesting the need for complementary measures
The remaining 20% mitigation gap could be filled by more efficient use of cement and concrete in the built environment through multi-stakeholder actions
including performance-based concrete design
could bring about additional emissions mitigation of 9% by 2050
Another 11% mitigation could be achieved through more intensive use of buildings and infrastructure
through such means as enhanced sharing practices and consolidation of urban functions
Extending the service life of buildings and infrastructure further provides additional mitigation of 4%
enabling even deeper and faster decarbonization
and stockpiling of demolition waste together all play a limited role in emissions reduction
but harmonize circular economy principles with deep decarbonization of the sector
our analysis reveals that it is technically possible to achieve net-zero emissions across the cement and concrete cycle by 2050 through the rapid and ambitious implementation of all the supply- and demand-side strategies spanning the entire value chain
The vertical dashed lines mark the year in which the future scenarios begin (2020)
while the bottom panel shows the total values
The vertical dashed lines mark the year in which the future scenario analyses begin (2020)
</a>The black lines mark the divisions between pathways that do (blue) and do not (red) achieve net-zero emissions
all supply-side strategies are expected to be implemented except CCS
our analysis calls into question the decarbonization roadmaps currently being proposed by government and industry
and emphasizes the importance of demand-side strategies to enable efficient use of cement and concrete in the built environment
in conjunction with supply-side strategies
Our analysis bridges this gap by providing a set of indicators
that are fully consistent with the net-zero emissions target
As demonstrated in the sensitivity analysis
since demand-side strategies and CCS can be complementary
placing the material flow-related indicators presented in this work as national targets will allow us to prepare for the risk of failure to scale up CCS sufficiently in time
It is important to note that our analysis does not negate the need for investment in CCS
this study emphasizes that the arsenal required to decarbonize the cement and concrete sector should include strategic options that go beyond just “silver bullet” supply-side technologies
material-efficient design and more intensive use need to be a priority in light of their high emissions-saving potential
From the perspective of consumption activities
the key intervention domain includes housing
These findings provide a window of opportunity for effective emission mitigation through targeted measures that enable and encourage consumers to seek out and adapt to material-efficient buildings and infrastructure in their consumption-related activities
the residual CO2 emissions will need to be reduced in other ways
Since this can clearly make a substantial difference in the design of strategies and investments
there is an urgent need to start and settle the debate on how to account for concrete-related CO2 uptake in national inventories under the Paris Agreement
the industry’s vision of achieving net-zero emissions will be inconsistent with that at the national level under the Paris Agreement
which will in turn jeopardize the effectiveness of net-zero emissions in the context of specific climate goals
now have an important opportunity to systematically converge their stock growth at a much lower level than existing developed countries by incorporating efficient use of cement and concrete into urban development planning
assuming the specific lifetime of each end-use category
this is a time-cohort-type approach that derives the in-use concrete stocks from the sum of the concrete inflows embedded in surviving buildings and infrastructure each year
the in-use stock of concrete is defined as the apparent quantity of material in buildings and infrastructure that are in-use in any given year
Note that since the focus of this study is on domestic demand
international trade is not taken into account here
This study considers 11 end-use categories of concrete based on national official statistics: (i) residential buildings; (ii) non-residential buildings; (iii) roads; (iv) landslide and flood control; (v) agriculture
forestry and fisheries; (vi) industrial water; (vii) sewerage; (viii) harbors and airports; (x) railways and telecommunications
residential and non-residential buildings are further broken down into six sub-categories according to structure type: wood
System variables and parameters are determined by aggregating various fragmented statistical data on cement
Some data are supplemented with information from previous MFA studies and interviews with industry representatives
A more detailed description of the modeling procedures and data sources is provided in Section 1.1 in the Supplementary Information
This study estimates the CO2 uptake by concrete carbonation considering four sinks: concrete
The progress of carbonization is explicitly modeled based on Fick’s law of diffusion
the carbonation rate of concrete and mortar is adjusted to account for the effects of exposed surface area
The carbonation rates of demolished concrete and mortar are modeled assuming that the waste particles are spherical
Uncertainties in CO2 uptake estimates are evaluated by Monte Carlo simulations in which each parameter is randomly extracted from a specific probability distribution
Details of the physicochemical model can be found in Section 1.3 of the Supplementary Information
using material intensity data for each application
dynamic changes in the flow and stock of buildings and infrastructure can be tied to a series of concrete and associated resource uses
We consider a total of 16 strategies to achieve net-zero emissions in the cement and concrete sector
These can be broadly classified into three categories: conventional supply-side strategies (six strategies)
emerging supply-side strategies (three strategies)
and demand-side strategies (seven strategies)
A brief description of each strategy group is given below
and barriers to implementation to be found in Section 3 of the Supplementary Information
the speed of implementation is assumed to be through linear growth from 2030 to 2050
implementation is envisioned to begin in 2021
as is the case with conventional supply-side strategies
It is important to note that the demand-side strategies considered here are not independent and that they influence each other
A prominent example is the strategies related to lifetime extension and several end-of-life options: the longer a concrete structure lasts through lifetime extension
the less end-of-life material is available for component reuse
Our model captures such interactions through a set of mass balance equations that ensure the feasibility of each strategy in terms of mass balance constraints
The interaction of strategies that do not depend on mass balance constraints is further explained in Section 3 of the Supplementary Information
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Implementing the material footprint to measure progress towards sustainable development goals 8 and 12
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Climate and health damages from global concrete production
Impacts of booming concrete production on water resources worldwide
The technological and social timelines of climate mitigation: lessons from 12 past transitions
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<a data-track="click" data-track-action="download citation references" data-track-label="link" rel="nofollow" href="https://citation-needed.springer.com/v2/references/10.1038/s41467-022-31806-2?format=refman&amp;flavour=references">Download references</a>
This research was supported in part by a Grant-in-Aid for Research (No
21K12344) from the Japanese Ministry of Education
and by the Environment Research and Technology Development Fund (JPMEERF20S20604 and JPMEERF20223001)
National Institute for Environmental Studies
Energy and Materials in Infrastructure and Buildings (EMIB)
Nature Communications thanks the anonymous reviewer(s) for their contribution to the peer review of this work. <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s41467-022-31806-2#MOESM2">Peer reviewer reports</a> are available
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations
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The guests also took some time to answer questions from the moderator and audience
revealing tidbits from the series production like the cast selection process—Watari said that almost all the actors were instantly selected
Three other candidates were in the running for the role
One audience member asked the panelists if there was any chance for more seasons
Watari to write some more,” to which Watari answered
“I'm definitely interested in writing more
so it is up to your support to motivate me,” which was met with thunderous applause
Another audience member mentioned that the club members in My Teen Romantic Comedy SNAFU spent a lot of time planning things that ultimately aren't that important
and asked if that happened during any part of the light novel or anime creation process
writing the novel was the same thing sometimes
A lot of effort on some things that were trivial.” Horii
who was decked out in a New York Yankees cap and enthusiastically used baseball metaphors in his answer
“I take what I can to the recording studio
My Teen Romantic Comedy SNAFU is very popular globally
When you all started on your individual journeys with this title—as a creator
actor—did you think you would be overseas with so many fans of this work
this anime is about a normal high school life in a relatively micro-ish environment centering around a single high schooler
We didn't expect that this would resonate with American students with their hardships
or with people around the world and their own hardships and ways of life
Horii: I did not expect this kind of popularity around the world either
and I love that I'm able to share this love for this anime with everyone here
I started on this project from the second season
the novel was already selling very well in Japan
but I also did not expect that this would be as much of a hit in North America too
I was able to get a confirmation of that by being invited here
Have you been surprised that things like comedy and romance are able to transcend language barriers so well
Watari: I actually did not expect that—with the comedy aspect especially—that the comedy would resonate with North American readers over here because the comedy I do is relatively Japanese in the sense that it focuses around word play
but seeing the magic that the translators and the editors did with the localized version
I was really impressed at the effort they took to adopt my comedy elements
Horii: I didn't expect the comedy elements to transcend language barriers either
I'm very happy that it did because now I'm able to share all those funny moments with the SNAFU family over here
I'm definitely happy those elements come across and everything
but I actually think it's due to the times too
because this anime was first animated ten years ago
let's say 20 years total—I wonder if the same elements would've been accepted in North America
North American fans may have gained a better understanding of Japanese anime in general
I think mediums like anime have really flattened some of the cultural barriers between countries
I think there's a little bit of privilege but also pressure to be able to be cultural ambassadors
or do you try not to think about that at all when you're making the product
Watari: There might be some pressure, but the fact that we're invited to the U.S. like this—we actually get fan letters from across the sea, too. What can I say? They're like a source of power for us! When you look on <a href="/encyclopedia/company.php?id=24886">Twitter</a> and similar platforms
and you just see how many people are excited at what we do
Maybe someday I could be a bridge to other parts of the world from Japan
I'm honored to have worked with such great content that people around the world have enjoyed that too
we get to see first-hand all the love that people show for this anime
come to a better understanding with each other
like the whole idea of “love and peace,” right
Kato: I am going to go at this from an anime producer's standpoint
I would like to keep the quality high for the things that I like
and the things that Japanese audiences like
but I would also like to be able to sell those kinds of things all around the world
We have some great games and everything over here in America
and I believe that one day—as a businessman—if I could also be here in the same market
and 2.5-D would be the realization of anime as live action on stage
 I am hoping that this gains popularity in North America as well
I actually read somewhere online that you (Watari) didn't participate in a lot of clubs in high school
Yet here is this massively popular series that revolves around an unusual club
Watari: Ummmmmmmmmmmm… well… if I didn't know who was in the club
I may have made a mistake somewhere in there
Did you find it challenging to find those tender moments amongst all the comedy
Watari: Although I personally didn't have any difficulty writing them
I think there can be discrepancies when readers or watchers think that this anime or this novel is just a comedy
and they see things like depressing scenes or romantic scenes and what not
There might be a discrepancy there between expectations
But I wanted to write a life story of these characters
and I don't think that comedy or romantic stories really stand up on their own
They're an integral part of a person's story
there are those points in life where you have those comedic moments as well as sad moments and happy moments
I wanted to write Hachiman's life and all the elements that are associated with it
If you're familiar with this line of Japanese drama series
and the great flow of history throughout time
I kind of wanted to write something like that
your character (Tobe Kakeru) is very interesting
Is that something you thought about while you were reading his lines
Horii: I think Tobe is kind of a mood-maker
There are moments that I think Tobe doesn't really show what his parts of the story are
but I think when he's in front of everyone
there's a concept called a “hype man.” Your character is like the ultimate hype man
I know there was a bit of a delay due to COVID
Did that make the production schedule more challenging
Not in the sense that there was one single person affected or anything
but more of a scheduling thing on a company-wide level
we had to delay the anime three months because of that
we were able to show the second season during that time period
so it actually ended up going okay in Japan
because I think people needed something to cheer them up during that time
Kato: If we did our part in making the world a better place
Would you rather be romantically rejected by your crush face-to-face
or have someone like Hachiman save you from rejection
and the fact that Hachiman remembered and saved Tobe… I'd like to be in that situation too
I have to say that I'd rather Hachiman come and intervene so I could be “Saaaaaaaaaafe!” (imitates a baseball umpire)
Think about it—there's going to be life after that rejection
A high school guy's heart is pretty easy to break
I don't want to carry over the trauma from that rejection
Watari: Thank you for supporting SNAFU for such a long time
a strange story that focuses on Japan in the small prefecture of Chiba—it's very specific to a country and place
So you in North America might see it as kind of an oddity at times
as things that go on in a very specific region in Japan
But this is actually modeled after a real location and everything
While we might still be locked up with COVID
maybe take that time to watch the series a few more times
and enjoy the basis of what became SNAFU in Japan
<a href="/articles/s42003-023-04543-5/metrics" data-track="click" data-track-action="view metrics" data-track-label="link" rel="nofollow">Metrics details</a>
Three-dimensional retinal organoids (3D-retinas) are a promising graft source for transplantation therapy
We previously developed self-organizing culture for 3D-retina generation from human pluripotent stem cells (hPSCs)
Here we present a quality control method and preclinical studies for tissue-sheet transplantation
Self-organizing hPSCs differentiated into both retinal and off-target tissues
Gene expression analyses identified the major off-target tissues as eye-related
we developed a qPCR-based test in which each hPSC-derived neuroepithelium was dissected into two tissue-sheets: inner-central sheet for transplantation and outer-peripheral sheet for qPCR to ensure retinal tissue selection
tissue-sheets were stored for 3–4 days using a newly developed preservation method
no transplant-related adverse events were observed
retinal transplants differentiated into mature photoreceptors and exhibited light responses in electrophysiology assays
These results demonstrate our rationale toward self-organizing retinal sheet transplantation therapy
The 3D-retinas in this culture system contain neural retinal (retinal) progenitors that expand to give rise to photoreceptors and other retinal neurons and form a multilayered stratified retinal tissue in a stage-dependent manner
These preclinical transplantation studies indicate that allogeneic PSC-derived retinal sheets have the potential to improve visual function
xenogeneic fluorescent protein knock-in reporter lines are not ideal for clinical applications
Here we establish a new QC strategy to select retinal tissue in the organoids
We then performed preclinical safety and efficacy studies to demonstrate the rationale for allogeneic retinal sheet transplantation therapy
</a>a Scheme of the self-organizing culture
b Bright-field view of iPSC-S17-derived cell aggregate containing retinal tissue on days 14
Immunostaining of iPSC-S17-derived retinal tissue on days 14
Crx (green) and Chx10 (red) in middle panels
Pax6 (green) and Recoverin (red) in lower panels
c Immunostaining of iPSC-S17-derived retinal tissue on day 180
RXRG (green) and NRL (red) in upper-left panel
CtBP2 (green) and Recoverin (red) in lower-left panel
Cone-arrestin (green) in lower-right panel
d Gene expression analysis in iPSC-S17-derived cell aggregates containing retinal tissue on days 0
mRNA levels were determined by qPCR analysis
Relative mRNA expression was determined by the delta-delta Ct method with GAPDH as an endogenous control
Data are presented as mean ± SE (n = 4 per time point)
e Bright-field view of iPSC-S17-derived retinal sheet
f Immunostaining of iPSC-S17-derived retinal sheet on day 87
Rx (green) and Recoverin (red) in right panel
human allogeneic iPSC-derived retinal sheets were reproducibly generated
</a>a Bright-field view of retinal tissue and off-target tissues in iPSC-LPF11-derived cell aggregates
b Proportions of cell aggregates containing retinal tissue and off-target tissues
Three independent batches derived from iPSC-LPF11 cells and three independent batches derived from iPSC-S17 cells were analyzed (n = 59–95 aggregates per each batch)
c Heat map for gene expressions in retinal tissue and off-target tissues derived from iPSC-LPF11 cells
gene expressions in undifferentiated iPSC-LPF11 cells are also shown
Gene expressions were measured by qPCR analyses
Row Z-scores were calculated for each tissue and plotted as a heat map
d Representative bright-field images and IHC images for off-target tissue-1 and -2 derived from iPSC-LPF11 cells
The bright-field images and IHC images were captured at the same positions
Immunostaining for Aqp1 (upper right; red) and Emx2 (lower right; red) is shown
e Heat map for gene expressions in retinal tissue and off-target tissue-3 derived from iPSC-1231A3 cells
mRNA levels were determined by microarray analysis
LogFC values were calculated for each gene and plotted as a heat map
Note that off-target tissue-3 expressed Hox genes compared with retinal tissue
hPSCs robustly differentiated to form a multilayered retinal tissue and the major off-target tissues were Aqp1+ eye-related tissue (off-target tissue-1)
Emx2+ cortex-like tissue (off-target tissue-2)
and HoxB2+ spinal cord-like tissue (off-target tissue-3)
</a>a Scheme for dissection of the cap and ring and the ring-PCR test in Process 5 (dissection)
right) images of a representative cap and ring
The cap and ring were derived from iPSC-LPF11 cells
c Heat map for gene expressions in caps and rings derived from iPSC-LPF11 cells
Row Z-scores were calculated for each cap and ring
d Representative gene expressions in cap and ring dissected from the same aggregate shown as a plot with a regression line (left)
The coefficient of determination (R2) for the gene expression between each cap and ring is shown (right)
e Violin plots of single cell PCR data obtained from a retinal sheet
Expressions of genes for photoreceptor cells (Crx
The continuous neuroepithelium, self-formed in iPSC-derived 3D-retina, was dissected into the cap and ring (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s42003-023-04543-5#Fig3">3a, b</a>)
The cap and ring were both found to contain a multilayered continuous retinal tissue comprising a photoreceptor precursor layer with Crx+ and Recoverin+ cells on the outer surface and a retinal progenitor cell layer with Chx10+ and Pax6+ cells on the inner side
These data indicate that the gene expression levels of retinal genes and off-target tissue-marker genes are almost same between the cap and ring dissected from the same aggregate
These results demonstrated that the ring-PCR test can estimate the gene expression in the cap to select retinal sheets comprised of retinal cells
subretinal transplantation of frozen retinal tissue in nude rats showed impaired engraftment
We therefore aimed to develop a non-freezing preservation method for retinal tissue
</a>a Screening for the non-freezing preservation conditions
+++: multilayered continuous retinal epithelium was preserved
++: multilayered continuous retinal epithelium was preserved but neural rosettes appeared
+: multilayered continuous retinal epithelium was observed but very limited
no multilayered continuous retinal epithelium was observed after the preservation
c Proportions of Crx+ photoreceptor precursors in multilayered retinal tissues
The proportions were calculated by dividing the number of Crx+ cells by the number of nuclei (DAPI)
Data are presented as mean ± SE for n = 5 (4 °C)
d Cell viability in non-preserved retinas (Control) and retinas preserved for 4 days
Data are shown as mean ± SE (n = 5 per group)
e Gene expressions of Chx10 and Crx in non-preserved retinas (Control) and retinas preserved for 4 days
Data are presented as mean ± SE (n = 3 per group)
f IHC analysis of retinas that were not preserved (d70 + 0)
preserved in Optisol for 2 days (d70 + 2) and 4 days (d70 + 4)
and preserved in Optisol for 4 days followed by recovery culture for 3 days (d70 + 4 + 3) and 7 days (d70 + 4 + 7)
g Numbers of EdU-positive cells per 100 µm-wide multilayered retinal tissue in each group
Data are presented as mean ± SE (n = 6 per group)
***p &lt; 0.001 for one-way ANOVA followed by a Tukey’s test
the preferable range of preservation temperature was 17 ± 5 °C and the optimal temperature was 17–22 °C
the mRNA levels of Chx10 and Crx in retinas preserved in Optisol at 17 °C for 7 days were comparable to those in control retinas in culture
These results demonstrated that non-freezing preservation in Optisol at 17 °C was the optimal condition for retinal tissue
The transplanted rats showed no abnormalities in their body weights, survival rates, and clinical signs compared with intact or sham-operated rats during the observation period of up to 78 weeks after transplantation (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/articles/s42003-023-04543-5#Fig5">5a, b</a>).
</a>Time courses of body weight (a) and Kaplan–Meier survival curves (b) in nude rats in the tumorigenicity study
Intact: control nude rat without surgery (n = 4)
Sham: control nude rat with subretinal injection of vehicle control (n = 4)
Transplanted: nude rat with subretinal transplantation of a single iPSC-Q-derived retinal sheet (n = 4)
d HE staining of transplanted eye sections at 13
e–h Immunostaining of transplanted eye sections for human nuclear markers
g High-magnification image of immunostaining for the graft (52 weeks) with antibodies for human Ku80 (green)
h Percentages of Ki67+ and Ku80+ cells among the Ku80+ human cells
the iPSC-Q-derived retinal sheets became engrafted at &gt;1.5 years after transplantation even under the xenotransplantation condition
no tumorigenicity and no other adverse effects related to the iPSC-Q-derived retinal sheets were observed
</a>a–j’ Immunostaining of rat eyes transplanted with iPSC-S17-derived retinal sheets
The retinal sheets were transplanted in the subretinal space of RD-nude rats
The rat retinas were fixed at 263 days after transplantation (341 days after initiation of differentiation)
Recoverin+ and PKCalpha+ rat bipolar cells
b–b” Immunostaining for human Ku80 (green)
High magnification in (b’) and higher magnification in (b”)
c–h’ Immunostaining for photoreceptor markers
S-opsin (green) and L/M-opsin (red) in (d)
g”): Synaptophysin-positive neurites in no nuclear space
Arrowheads in (h’): Calbindin-positive neurites
i’ Maximum projection image of Z-stacks immunostained for human Ku80 (green)
Note that human Ku80− and PKCalpha+ bipolar cells were located near the human Ku80+ and Recoverin+ photoreceptors (arrowheads)
j’ Maximum projection image of Z-stacks stained for CtBP2 (green)
Note that photoreceptor-synapse marker CtBP2 and LRIT3 were expressed near the neurites of PKCalpha+ rod bipolar cells
These results demonstrated that preserved retinal sheets became engrafted into severe RD-model immunodeficient rats to differentiate into mature photoreceptors with OS-like and OPL-like structures
</a>a Scheme of ex vivo electrophysiology assay using MEA to evaluate the light responses in transplanted iPSC-retinas
iPSC-S17-derived retinal sheets were transplanted in the subretinal space in RD-nude rats
Transplanted and non-transplanted rat retinas were mounted on the MEA electrodes for electrophysiological recording
light stimuli were conducted in different intensities (weak
and the rat retinas were incubated in Ames’ medium (Before)
b–d Representative results of MEA analysis
(b) Differential interference contrast (DIC) image of a RD-nude rat retina mounted on the MEA
Engrafted area was indicated by red dotted line
d Peri-stimulus time histograms to examine the RGC-spike counts during strong light stimuli (12.84 log photons/cm2/s)
The X-axis and Y-axis represent the time and average RGC-spike count
Recording data for iPSC-S17-derived retinal sheet-transplanted rat retinas (Transplanted; upper) and non-transplanted rat retinas (Non-transplanted; lower) in the three conditions (Before
Multiple electrode recording data are shown in (c) and representative data (red box in c) are shown in (d)
Red arrows in (d): start timing of light stimuli
Control non-transplanted rat retinas (Control; n = 12) and iPSC-S17-derived retinal sheet-transplanted rat retinas (Transplanted; n = 12) were subjected to MEA analysis to evaluate the RGC responses to light stimulation
Dots and bars show the per sample (recorded rat retina) summary of the collected data
Dot: RGC light response probability estimated by statistical modeling (Bayesian statistical inference with Markov Chain Monte Carlo sampling)
The key remaining technologies for tissue/organoid transplantation were a QC strategy and a preservation method
we developed a QC strategy for hPSC-derived retinal sheets
Retinal tissue in culture self-forms a unique morphological structure that is easy to distinguish under a microscope
and therefore retinal sheets can be dissected
To exclude possible errors in the selection of retinal tissue and avoidance of off-target tissues
we characterized the major off-target tissues and developed the ring-PCR test
Retinal tissue with its large continuous neuroepithelial structure can be dissected into two tissue-sheets: the inner-central sheet (cap) and the outer-peripheral sheet (ring) with similar gene expression patterns
qPCR analysis of the ring can be used to estimate the gene expression in the corresponding cap
As the ring-PCR test requires 1–2 days to complete
we developed the RT-preservation method to store retinal sheets for at least 3–4 days
To demonstrate the rationale for the QC strategy and the RT-preservation method
we performed in vivo tumorigenicity and preclinical efficacy studies
no tumors or no other adverse effects related to the iPSC-derived retinal sheets were observed
The preclinical efficacy study demonstrated that preserved retinal sheets had the potential to become engrafted and mature in the severely degenerated condition and exhibited light response functions measured by ex vivo electrophysiology assays
These preclinical studies support the concept of iPSC-derived retinal-sheet transplantation for retinal degeneration
Kobe City Eye Hospital applied for clinical research for a limited number of patients using iPSC-derived retinal sheets for retinitis pigmentosa
the clinical research plan was approved by the Health Science Council at the Ministry of Health
and the clinical research was started (Trial ID: jRCTa050200027)
Sumitomo Pharma developed a clinical grade manufacturing process and generated clinical grade allogeneic iPSC-derived retinal sheets
iPSC-retinal sheets were transplanted in two patients in 2020–2021
This feature of 3D self-organization possibly based on the intrinsic embryonic development program is the core technology for robust generation of retinal sheets with controlled quality
automation and mechanization of the dissection process will be important for future manufacture of iPSC-derived retinal sheets
imaging technologies and computational image processing
are promising approaches for the development of non-destructive QC tests
we would like to proceed with single cell analysis and imaging technology to further investigate the quality of the self-organizing 3D-retinas
We developed an RT-preservation method and performed transplantation studies
for retinal sheets under the RT-preservation condition
Toward future clinical applications in large numbers of patients
the large animal studies to develop surgical procedures
and immunosuppression protocols are important to enable widespread use of retinal sheets
Based on the self-organizing stem cell culture technology
the realization of tissue/organoid therapy in medicine is ongoing
The experimental protocols using clinical grade human iPSCs were approved by the Research Ethics Committee of Sumitomo Pharma Co.
iPSCs were treated with SB431542 (SB; Wako) and/or smoothened agonist (SAG; Enzo Biochem) as the preconditioning step
the culture medium was changed every 3–4 days to generate immature retinal tissue
the immature retinal tissue generated from iPSCs was subjected to a two-step induction-reversal culture as follows
cell aggregates on days 14–18 were transferred from 96-well plates to 90-mm non-cell-adhesive petri dishes (Sumitomo Bakelite; approximately 32–48 aggregates/90-mm dish)
and cultured for 3 days in DMEM/F12-Glutamax medium (Gibco) containing 1% N2 supplement (Gibco)
For the reversal culture (Process 3) and the maturation culture (Process 4)
the cell aggregates were cultured in retina maturation medium as described (Nukaya et al
The medium was changed every third or fourth day to obtain 3D-retinas
Floating cell aggregates were analyzed using an inverted microscope (Keyence BZ-X810
The dissected inner-central neuroepithelial tissue sheet was collected as the cap
the surrounding outer-peripheral part of the neuroepithelial tissue was cut from the cell aggregate and harvested as the ring
The obtained caps and rings were subjected to the RT-preservation method and the ring-qPCR test
Caps whose rings passed the ring-PCR test were used as retinal sheets for transplantation
Rings and other tissue samples were lysed with Buffer RLT (Qiagen) containing 1% 2-mercaptoethanol
and total RNA was extracted and purified using an RNeasy Micro Kit (Qiagen)
The total RNA was reverse-transcribed and subjected to qPCR using a StepOne Plus Real-Time PCR System (Applied Biosystems) or Biomark HD (Fluidigm) according to the manufacturers’ instructions
Z-scores were calculated for the individual caps or rings
Data were visualized as heat maps using the heatmap.2 function of the R/Bioconductor package gplots
PCA was performed using the prcomp function in the R base package
The PCA results were visualized using R package ggplot2
The following qPCR primers (TaqMan probes) were purchased from Applied Biosystems
and used for qPCR analysis: GAPDH (Hs02758991_g1)
Dissected tissues in the cell aggregates were lysed with Buffer RLT (Qiagen) containing 1% 2-mercaptoethanol
Microarray analysis using a GeneChip array was performed by Kurabo Industries (Osaka
total RNA was reverse-transcribed to cDNA with T7 oligo d(T) primer (Affymetrix)
biotin-labeled cRNA was synthesized and amplified by in vitro transcription of the second-strand cDNA template using T7 RNA polymerase (Affymetrix)
The labeled cRNA was purified and fragmented
loaded onto a GeneChip® Human Genome U133 Plus2.0 array (Affymetrix)
and hybridized according to the manufacturer’s protocol
Raw intensity data from the GeneChip array were analyzed using GeneChip Operating Software (Affymetrix)
The data were logarithmically transformed and logFC was calculated for each gene
The logFC data were visualized as heat maps using the heatmap.2 function of the R/Bioconductor package gplots
Caps and rings were dissected from 3D-retinas
The cap whose ring passed the ring-PCR test was subjected to scPCR analysis
The cap was dissociated into single cells by using papain (Wako)
followed by loaded into the C1 preamp IFC (10–17 μm
Pre-amplification of genes was performed in the C1 instrument (Fluidigm) according to manufacturer’s instructions
The pre-amplified cDNAs from each cell were further amplified by using the Biomark HD instrument (Fluidigm) with the TaqMan assays
Cell aggregates and tissue sheets (caps) were transferred to 1.5-mL or 15-mL tubes
preservation solution was added and the tubes were placed in incubators set at 4
the temperatures of the incubators were monitored by temperature loggers in some experiments
Belzer UW Cold Storage Solution (Bridge to Life)
each 1.5-mL tube containing a cap in Optisol was placed in a cool incubator (Mitsubishi Electric Engineering) set at 17 °C
including the ring-PCR test were picked up and used for transplantation
Tissue sheets which were dissected from 3D-retinas were dissociated into single cells by using papain (Wako)
After centrifugation and removal of supernatants
dissociated cells were suspended in fresh media
Total cells and dead cells were stained by acridine orange and DAPI
and the number and the percentage of viable cells were determined by using NucleoCounter® NC-200 (ChemoMetec)
Fundus imaging and OCT analysis were performed using RS-3000 Advance (Nidek) and Micron IV and OCT (Phoenix research labs) according to the manufacturers’ instructions
the eyeballs were fixed at 4 °C in SUPERFIX (KY-500; Kurabo Japan)
and sliced with a microtome at 3-µm thickness
One in every five sections was used for HE staining and histopathological analysis
and the other sections were used for immunohistochemistry
IHC was performed as described<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 8" title="Kuwahara, A. et al. Preconditioning the initial state of feeder-free human pluripotent stem cells promotes self-formation of three-dimensional retinal tissue. Sci. Rep. 9, 18936 (2019)." href="/articles/s42003-023-04543-5#ref-CR8" id="ref-link-section-d462790059e2628">8</a>
Cell aggregates and transplanted retinas harvested from RD-nude rats were fixed with 4% paraformaldehyde (Wako) and sectioned with a cryostat (Leica) to prepare frozen sections
Frozen sections and paraffin sections were treated with or without heat-based antigen retrieval in Target Retrieval solution (Dako) at 105 °C for 15 min
The primary antibodies used were as follows: anti-Chx10 (sheep; 1:500; Exalpha)
anti-Recoverin (rabbit; 1:500; Proteintech)
anti-RXRG (mouse; 1:500; Santa Cruz Biotechnology)
anti-Rhodopsin (mouse; 1:1000; Sigma Aldrich)
anti-S-opsin (goat; 1:1000; Santa Cruz Biotechnology)
anti-S-opsin (rabbit; 1:500; Santa Cruz Biotechnology)
anti-Cone-arrestin (Arrestin-3; goat; 1:500; Novus)
anti-PKCalpha (goat; 1:500; R&amp;D Systems)
anti-Aqp1 (Aquaporin1; rabbit; 1:500; Millipore)
anti-cleaved caspase-3 (rabbit; 1:200; Cell Signaling Technology)
anti-human Ku80 (rabbit; 1:500; Cell Signaling Technology)
anti-human Ku80 (goat; 1:500; R&amp;D Systems)
anti-Gnat1 (rabbit; 1:500; Santa Cruz Biotechnology)
anti-Gnat2 (rabbit; 1:500; Santa Cruz Biotechnology)
Peanut agglutinin (PNA) (1:500; Thermo Fisher Scientific)
anti-Synaptophysin (goat; 1:500; R&amp;D Systems)
Nuclear counterstaining was performed with DAPI (Nacalai)
Stained sections were analyzed with a fluorescence microscope (Keyence BZ-X810) or a laser scanning confocal microscope (Olympus Fluoview FV1000D
Image analyses were performed with IMARIS (Oxford Instruments)
and Zen Blue (Carl Zeiss) imaging software
control retinas before preservation (d70 + 0)
retinas preserved in Optisol at 17 °C for 2 days (d70 + 2) and 4 days (d70 + 4)
and retinas recovered in culture at 37 °C for 3 days (d70 + 4 + 3) and 7 days (d70 + 4 + 7) were treated with the thymidine analog EdU for 4 h
The EdU-treated retinas were fixed and sectioned with a cryostat (Leica)
Sections of the retinas were stained with Alexa Fluor 488-labeled azide (Invitrogen) according to the manufacturer’s protocol
Images were obtained using a confocal laser microscope and the numbers of EdU-positive cells and DAPI-positive nuclei were counted using ImageJ software (National Institutes of Health)
Statistical analyses were performed with R version 3.6.0 (The R Foundation for Statistical Computing)
A two-tailed Student’s t-test was carried out for two-group comparisons
and one-way analysis of variance (ANOVA) followed by Tukey’s test was performed for multiple-group comparisons
Intergroup differences in body weight and survival rate were tested by one-way ANOVA and the log-rank test
Further information on research design is available in the <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s42003-023-04543-5#MOESM4">Nature Portfolio Reporting Summary</a> linked to this article
All reasonable requests will be promptly reviewed by the senior authors to determine whether the request is subject to any intellectual property or confidentiality obligations. This study did not generate new cell lines. Source data for graphs is provided as <a data-track="click" data-track-label="link" data-track-action="supplementary material anchor" href="/articles/s42003-023-04543-5#MOESM3">Supplementary Data</a>
Microarray transcriptome data are available with accession number GSE197446
Next-generation regenerative medicine: organogenesis from stem cells in 3D culture
Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity
Generation of Rx+/Pax6+ neural retinal precursors from embryonic stem cells
Toward the generation of rod and cone photoreceptors from mouse
Self-organizing optic-cup morphogenesis in three-dimensional culture
Self-formation of optic cups and storable stratified neural retina from human ESCs
Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue
Preconditioning the initial state of feeder-free human pluripotent stem cells promotes self-formation of three-dimensional retinal tissue
Efficient generation of retinal progenitor cells from human embryonic stem cells
Transplantation of human embryonic stem cell-derived photoreceptors restores some visual function in Crx-deficient mice
Modeling early retinal development with human embryonic and induced pluripotent stem cells
Optic vesicle-like structures derived from human pluripotent stem cells facilitate a customized approach to retinal disease treatment
Reproducibility and staging of 3D human retinal organoids across multiple pluripotent stem cell lines
Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs
Photoreceptor outer segment-like structures in long-term 3D retinas from human pluripotent stem cells
Brief report: self-organizing neuroepithelium from human pluripotent stem cells facilitates derivation of photoreceptors
Photoreceptor precursors derived from three-dimensional embryonic stem cell cultures integrate and mature within adult degenerate retina
Recapitulation of human retinal development from human pluripotent stem cells generates transplantable populations of cone photoreceptors
From confluent human iPS cells to self-forming neural retina and retinal pigmented epithelium
Generation of storable retinal organoids and retinal pigmented epithelium from adherent human iPS cells in xeno-free and feeder-free conditions
Efficient stage-specific differentiation of human pluripotent stem cells toward retinal photoreceptor cells
IGF-1 signaling plays an important role in the formation of three-dimensional laminated neural retina and other ocular structures from human embryonic stem cells
Cell replacement and visual restoration by retinal sheet transplants
Photoreceptor cell replacement in macular degeneration and retinitis pigmentosa: A pluripotent stem cell-based approach
Restoration of visual function in advanced disease after transplantation of purified human pluripotent stem cell-derived cone photoreceptors
Vision improvement in retinal degeneration patients by implantation of retina together with retinal pigment epithelium
Retinal repair by transplantation of photoreceptor precursors
Restoration of vision after transplantation of photoreceptors
Material exchange in photoreceptor transplantation: updating our understanding of donor/host communication and the future of cell engraftment science
Immunosuppression via loss of IL2rgamma enhances long-term functional integration of hESC-derived photoreceptors in the mouse retina
Characterization and transplantation of CD73-positive photoreceptors isolated from human iPSC-derived retinal organoids
Transplantation of embryonic and induced pluripotent stem cell-derived 3D retinal sheets into retinal degenerative mice
iPSC-derived retina transplants improve vision in rd1 end-stage retinal-degeneration mice
Establishment of immunodeficient retinal degeneration model mice and functional maturation of human ESC-derived retinal sheets after transplantation
Medium- to long-term survival and functional examination of human iPSC-derived retinas in rat and primate models of retinal degeneration
Transplanted hESC-derived retina organoid sheets differentiate
and improve visual function in retinal degenerate rats
Low immunogenicity and immunosuppressive properties of human ESC- and iPSC-derived retinas
Transplantation of human embryonic stem cell-derived retinal tissue in two primate models of retinal degeneration
Self-organized formation of polarized cortical tissues from ESCs and its active manipulation by extrinsic signals
A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells
Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells
Aquaporins in complex tissues: distribution of aquaporins 1-5 in human and rat eye
Aquaporin-1 channels in human retinal pigment epithelium: role in transepithelial water movement
Wnt and Bmp signalling cooperatively regulate graded Emx2 expression in the dorsal telencephalon
Neuronal defects in the hindbrain of Hoxa1
Hoxb1 and Hoxb2 mutants reflect regulatory interactions among these Hox genes
HoxB2 binds mutant SOD1 and is altered in transgenic model of ALS
Highly sensitive in vitro methods for detection of residual undifferentiated cells in retinal pigment epithelial cells derived from human iPS cells
Hypothermic temperature effects on organ survival and restoration
A comparison of the preservation of mouse adipose tissue-derived mesenchymal stem cells using the university of Wisconsin Solution and Hank’s balanced salt solution
The corneal epithelium after optisol-GS storage
Tumorigenicity studies of induced pluripotent stem cell (iPSC)-derived retinal pigment epithelium (RPE) for the treatment of age-related macular degeneration
First-in-human clinical trial of transplantation of iPSC-derived NS/PCs in subacute complete spinal cord injury: Study protocol
Retinal transplantation-induced recovery of retinotectal visual function in a rodent model of retinitis pigmentosa
A new immunodeficient pigmented retinal degenerate rat strain to study transplantation of human cells without immunosuppression
Genetically engineered stem cell-derived retinal grafts for improved retinal reconstruction after transplantation
Retina organoid transplants develop photoreceptors and improve visual function in RCS rats with RPE dysfunction
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
Self-formation of functional adenohypophysis in three-dimensional culture
Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue
CRISPR generated SIX6 and POU4F2 reporters allow identification of brain and optic transcriptional differences in human PSC-derived organoids
A novel approach to single cell RNA-sequence analysis facilitates in silico gene reporting of human pluripotent stem cell-derived retinal cell types
Deconstructing retinal organoids: single cell RNA-Seq reveals the cellular components of human pluripotent stem cell-derived retina
Cell types of the human retina and its organoids at single-cell resolution
Single-cell transcriptomic comparison of human fetal retina
Critical functionality effects from storage temperature on human induced pluripotent stem cell-derived retinal pigment epithelium cell suspensions
Room temperature shipment does not affect the biological activity of pluripotent stem cell-derived retinal organoids
Sheets of human retinal progenitor transplants improve vision in rats with severe retinal degeneration
A clinical-grade HLA haplobank of human induced pluripotent stem cells matching approximately 40% of the Japanese population
Pre-clinical study of induced pluripotent stem cell-derived dopaminergic progenitor cells for Parkinson’s disease
Design of a tumorigenicity test for induced pluripotent stem cell (iPSC)-derived cell products
<a data-track="click" data-track-action="download citation references" data-track-label="link" rel="nofollow" href="https://citation-needed.springer.com/v2/references/10.1038/s42003-023-04543-5?format=refman&amp;flavour=references">Download references</a>
Mototsugu Eiraku for advice on retinal maturation culture
and members of RACMO for fruitful discussions
would like to express deep gratitude to his late mentor Dr
a gifted scientist who pioneered self-organizing stem cell biology
This work was supported by the Research Center Network for Realization of Regenerative Medicine from the Japan Agency for Medical Research and Development (AMED) (M.T.
These authors contributed equally: Kenji Watari
Regenerative &amp; Cellular Medicine Kobe Center
RIKEN Center for Biosystems Dynamics Research
Research &amp; Development Center for Cell Therapy
Foundation for Biomedical Research and Innovation at Kobe
Technology Research &amp; Development Division
RIKEN Program for Drug Discovery and Medical Technology Platforms
designed the study for off-target tissue analysis
designed the study for self-organizing culture and IHC analysis in vivo
designed and performed electrophysiology assay
designed and performed tumorigenicity study
supervised and designed in vivo tumorigenicity study
supervised and designed in vivo efficacy study
supervised and designed self-organizing culture
This work was funded by AMED under grant number JP21bm0204002 (M.T.
has a scientific consulting role for Sumitomo
have received research funding from Sumitomo
The authors are co-inventors on patent applications
The remaining authors declare no competing interests
reviewer(s) for their contribution to the peer review of this work
Primary Handling Editors: Simona Chera and Eve Rogers
<a data-test="citation-link" data-track="click" data-track-action="download article citation" data-track-label="link" data-track-external="" rel="nofollow" href="https://citation-needed.springer.com/v2/references/10.1038/s42003-023-04543-5?format=refman&amp;flavour=citation">Download citation</a>
DOI: https://doi.org/10.1038/s42003-023-04543-5
Acta Neuropathologica Communications (2024)
The manga <a href="/news/2023-08-24/watari-kun-is-about-to-collapse-manga-ends-in-september/.201572">ended</a> with its final two chapters in the same issue
Narumi <a href="https://natalie.mu/comic/news/120458" target="_blank">launched</a> the manga in <a href="/encyclopedia/company.php?id=15420">Kadokawa</a>'s <a href="/encyclopedia/manga.php?id=12495">Young Ace</a> magazine in August 2014. The manga <a href="https://natalie.mu/comic/news/166652" target="_blank">moved</a> its serialization to Monthly Young Magazine in November 2015
Kodansha published the manga's first compiled book volume in May 2016
Source: Monthly Young Magazine October issue
In November 2017 when Duane Watari found boxes of poetry written in the Japanese form of senryu by his grandfather during World War II
he immediately sought help in translating it
That emailed plea landed on Shelley Baker-Gard’s lap as she just happened to be traveling by train from Nara to Kyoto
The Oregon Coordinator of the Haiku Society of America (HSA) agreed to help
What followed is a tale of what some might call serendipity
This month – six years after Watari wrote that first note -- Oregon State University Press debuts the book inspired by his find
“They Never Asked - Senryu Poetry from the WWII Portland Assembly Center.”
It is a tale that might as easily been lost as found had it not been for Watari’s careful eye on a day in 2017 as he cleaned out the basement of his mother’s Portland home in preparation for its sale
but she was an organized hoarder,” Watari said
You could tell she had some stuff sorted out.”
separating the contents into the things he would keep
those he would toss — and the ones calling for a closer look – like the notebooks and tablets containing his grandfather’s writing
There were poems in Japanese and diaries in English and one tablet
written vertically along one side were the words: “W.C.C.A
2.” Inside were pages and pages of senryu poetry – all written in Japanese and all utterly unintelligible to Watari
but not read or write it.) Though he couldn’t read the writing
and I’ll bet they are from when they were imprisoned.’ I felt this sense of
here is this piece of history that needs to be told.”
For Watari’s family and more than 3,600 Japanese Americans from Oregon and Washington
that piece of history began in March 1942 when the Wartime Civil Control Administration gave them 48 hours to gather their belongings
operated a small business in Portland selling seasonal goods like produce and bedding plants
That ended when they were taken to the Portland International Livestock Exposition Center
where they were housed in what had been animal stalls
they were transferred to the newly built camp in Minidoka
They were returned home after the war ended in 1945 and resumed the family business
though the property had been extensively vandalized during the war
Watari’s family didn’t talk about those years
“It was known after the war that the Japanese community did not want to talk about what happened
standing in the basement of the house his parents bought in Northeast Portland in 1959
Watari held in his hands the truth of what it is to be forcibly taken from your home
imprisoned and left to live every day in fear
Watari searched the internet for “senryu,” but found nothing locally
so he tried “haiku.” Both Japanese poetry forms have a 5-7-5 rhythm
but while haiku are generally about nature and the nonhuman world
senryu tend toward human relationships and the contemporary world
I’d love to take a look at the journals and see what we can do,” Baker-Gard told him
Baker-Gard reached out to Watari to set a date to meet and promised to look for someone to translate the work
the script was not modern and would be difficult to translate
“Then,” Baker-Gard writes in the introduction of ‘They Never Asked,’ “serendipitously
Michael Freiling came to the Portland Haiku Group meeting
“I’d recently started going back to Japan for the first time in 25 years
I thought it would be interesting to find out who was writing haiku at the time
Freiling was a Luce Scholar – a competitive fellowship program that offers immersive experiences in Asia – and spent time in Japan in the 1970s
his fluency in Japanese and experience in translation
I literally cornered him,” Baker-Gard recalled
and Baker-Gard fully understood the significance of what he’d found
“I just felt a very strong emotional response to it,” she said
“I felt this had to be what they were experiencing in the moment because that’s what this type of poetry form relates
and you are describing them in a very brief way
I knew they had to be experiencing conflicting emotions because they were rounded up and put in this assembly center
I was awestruck as it dawned on me that I was holding a piece of history in my hands
who was born and raised in Kyoto and teaches English in Japan
Some of the poems were written by Watari’s grandfather
who wrote under the pen name of Jōnan; other poems were penned by fellow members of the Bara Ginsha Senryu group
The first poem Freiling had read was one chosen at random when Baker-Gard dropped by his office
It was also the first he and Takikawa translated
*The train was departing to the Minidoka Idaho incarceration camp between August and September
“My experience with this was pretty much overwhelming,” Freiling said
sensing the awesome sense of how we’re human beings who want the same things and are all trying our best
It’s really inspiring to see how these people persevered in these horrible conditions
If you were to ask me to describe my feelings in a single word
Although Watari’s family didn’t talk about the war years
would be happy the book has been published
“He would feel proud to have something translated
but so everybody could understand what they felt when they wrote these poems
They expressed real feelings about what was going on
They talked about other families; what others are doing in the camp
I’m just going to call it the luck of the draw that I just happened to find this.”
— Lori Tobias is the author of “Storm Beat: A Journalist Reports from the Oregon Coast,” published by Oregon State University Press
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Watari-kun no xx ga Hokaisunzen (Watari-kun’s ****** Is About to Collapse) will receive an anime adaptation
The announcement came in the October edition of Kodansha’s Monthly Young Magazine
where the manga ended its serialization with two final chapters
<a href=https://kodansha.us/series/watari-kuns-is-about-to-collapse>Kodansha USA </a>publishes the manga digitally in English
Naoto’s ordinary life is thrown into chaos … and Satsuki may just be the beginning.Naru Narumi first launched Watari-kun’s ****** Is About to Collapse in August 2014
and the series has since been collected into fifteen volumes
with fourteen currently available in English
No further information about the anime adaptation has been announced at this stage
the Japanese hairstylist opened up his London salon last year
Words: <a href="https://theface.com/contributors/jade-wickes" class="contributors__name tw-no-underline hover:tw-underline">Jade Wickes</a>12th July 2021
When hairdresser extraordinaire <a href="https://www.instagram.com/keisuke_watari_/">Keisuke Watari</a> moved from Japan to London in 2019
he’d already undergone five years of intensive training in his home country
So when the UK’s first lockdown was lifted last April
Watari was well placed to open up a&nbsp;salon in Bethnal Green
Nameless and websiteless for the time being
his Instagram account and word of mouth do the talking
and it seems Londoners can’t get enough of Watari’s electrifying cuts
and between being an artist and a&nbsp;commercial hairdresser,” the 30-year-old says
​“I don’t see being a&nbsp;hairdresser as art – I&nbsp;work with real people
These things create chemistry and a&nbsp;one-of-a-kind
the hairdresser possesses an undeniable artistic flair
finding references in both the historical and contemporary
you’ll find a&nbsp;take on Chinese queue hair
an ancient style worn by Manchurians since the 1600s
hot pink mohawk and bright red cross sprayed onto bleach-blonde hair
and post-mullets that look just the right amount of lived-in
dreams and encountering things I&nbsp;don’t like,” he continues
​“Most of my customers leave it up to me to decide what style they’ll get
I&nbsp;want to translate their vibe after spending time together and make the hairstyles as alive as possible.”
Though the ripple effects of lockdown came down hard on <a href="https://theface.com/life/generation-beauty-nails-aesthetics-botox-skincare-coronavirus-lockdown">the beauty industry</a>
it was one of the most memorable periods of Watari’s life
He visited clients in their homes and was grateful for the opportunity to get to know them better
​“It made me feel like we were going through this difficult time together,” he says
​“Working with these people is one of the main reasons I&nbsp;want to stay in the UK.” 
but he isn’t always happy with the final outcome
​“I still have a&nbsp;lot of areas that I&nbsp;don’t like about my own look
but that sensitivity allows me to see and understand my clients’ own complexities,” he explains
“I also don’t want to have a&nbsp;signature style
That doesn’t mean I&nbsp;want to strike a&nbsp;balance
though – I&nbsp;want to be able to create both extreme energies.”
Watari hopes to open up a&nbsp;second salon in Japan
in a&nbsp;bid to further blend British and Japanese hairstyles with one another: ​“I feel as though both sides misunderstand each other
2022A Watari-um Trip Through Our Visual-centric ExistenceExploring The Watari Museum of Contemporary Art&#x27;s Collection Installation View of &quot;I Love Art 16: Vision – Focusing on Drawings by 15 Artists Including Warhol
<a href="https://www.tokyoartbeat.com/en/events/-/2022%2Fi-love-art-16-vision-focusing-on-drawings-by-15-artists-including-warhol-beuys-and-paik" target="_blank" rel="noreferrer">I Love Art 16: Vision – Focusing on Drawings by 15 Artists Including Warhol, Beuys, and Paik</a> at The Watari Museum of Contemporary Art (Watari-um) until May 15th reflects the  influence of the pandemic on our visual experiences
Meetings now happen via computer and mobile phone
Our eyes have become accustomed to browsing screens rapidly and seemingly endlessly
One outcome of this change could be an invigorated capacity to see and perceive images
We may even be shifting toward shared visions that create collective interpretations of information
Another way to stimulate our optical sensors and emotional instincts is by viewing art
Through 160 drawings and paintings by 15 world-renowned artists such as Andy Warhol
this exhibition aims to impart visual experiences that enrich our sense of sight
Three plaster sculptures by Keith Haring demonstrate the artist’s trademark free-flowing lines and bold color manipulation: Untitled (toruso) (1983)
Untitled (cubic face–red &amp; yellow) (1983)
and Untitled (cubic face–red &amp; green) (1983)
These works come from Haring’s first Japan exhibition in 1983
and on building walls around Harajuku and Aoyama
Haring once stated that his drawings do not convey any specific message
but rather a more direct intention of spreading happiness to people of all ages and races around the world
Video art pioneer Nam June Paik used television as a conduit for live media that could connect people
Paik drew two flowers with a crayon and pen
one in a flower pot labeled “Flux” and another on a TV monitor
“Flux”  is derived from “fluxus,” a Latin word denoting “flow” and the name of an international art movement of the 1960s–1970s to which Paik belonged
Members produced experimental works developing new forms of art
Paik noted: &quot;Once you are on videotape
you are not allowed to die.&quot; The exhibition text
responds: &quot;Will the flowers appearing on the monitor be allowed to wither?&quot;
the exhibition also displays two eye-catching suspended lamps: Homage to Paul Schatz (2012) by Olafur Eliasson and I dreamed I found a red ruby (1986) by Jonathan Borofsky
Eliasson’s work is made of optical lighting film
and LED strips enclosing two orthogonal circles
an engineering concept developed by scientist Paul Schatz
The design philosophy is based on the idea that “nature is sometimes inside and sometimes outside.” Eliasson exaggerates the shape by elongating it
so that it appears to be pushing itself apart from within
Borofsky’s beaming ruby-red sculpture is based on a dream
He transposed the intense image of the ruby from a drawing to a sculpture
Contemporary Japanese artists from the Watari-um collection featured in the show include Ryoko Aoki
Participating as a guest artist is Hiraki Sawa
Subscribers to the <a href="https://www.tokyoartbeat.com/en/aboutApp" target="_blank" rel="noreferrer">Tokyo Art Beat app</a> are eligible for ¥300 admission discounts to this show with MuPon museum coupons
Located not far from the chic district of Ometosando
its exhibitions and retrospectives will delight all
The Watari Museum is a private institution, located in the ward of Shibuya, not far from the popular areas of <a href="https://www.japan-experience.com/all-about-japan/tokyo/attractions-excursions/harajuku" target="_blank">Harajuku</a> and <a href="https://www.japan-experience.com/all-about-japan/tokyo/attractions-excursions/omotesando-0" target="_blank">Omotesando</a>
which now shines on the international contemporary art scene
was opened in 1990 at the will of Shikusho Watari
an art enthusiast belonging to a large family
It was the Swiss architect Mario Botta who was chosen in 1985 to design the building that would house the museum
The latter is very well known for his modern designs of churches and cathedrals
and in particular for the design of the Museum of Modern Art in San Francisco
The museum now stands on a triangular plot
while the ground floor houses a café and a bookstore
The top floors are reserved for staff and the private residence of the owners
This museum focusing only on contemporary art does not present permanent collections but organizes temporary exhibitions
generally centered on such or such contemporary artists
The museum presents contemporary art through all media: painting
This private museum has given itself the mission of promoting contemporary art in Japan
This unique space gives voice to both emerging and famous creators from the art world
He is also interested in the place of Japanese artists on the contemporary scene&nbsp;and strives to create links between Japanese and Asian artists
The Watari Museum thus regularly invites curators
and curators to organize exhibitions highlighting international artists who are committed to the development of contemporary art
Retrospectives of major artists such as Joseph Beuys
or Henry Darger have been offered in its rooms
who notably became known for having made the Louvre pyramid "disappear" in 2016
was notably exhibited at the Watari Museum in 2013
the American artist John Lurie is highlighted in an exhibition entitled "Walk this Way"
      The Watari Museum of Contemporary Art
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