the car was a crucial part of the getaway after the thieves who had previously stolen a gray Volkswagen Golf set the car on fire in Rolde before switching to the bright yellow Peugeot Police spokespersons confirmed the investigation into the car's use "There has indeed been extensive investigation into the yellow Peugeot." The Peugeot 208 was reportedly rented by a 36-year-old man from Alkmaar was unaware of its intended use in the heist claims he was asked to rent the car for an unrelated purpose "He was told to pick up items from Marktplaats in Hilversum and later asked to arrange a vehicle for a move," said his lawyer Police confirmed that after the car was left at Beilen who later abandoned it near the Grolloërstraat carpool area in Rolde Authorities had kept details of the yellow Peugeot’s involvement under wraps until now The suspect from Alkmaar was arrested on April 15 in connection with the heist and held in custody for nine days although he remains a suspect in the ongoing international investigation Duin has maintained that his client had no knowledge of the art theft he wouldn't have gotten involved," Duin emphasized noting that his client only realized the connection between the car and the crime after seeing media coverage of the heist at least three criminals broke into the Drents Museum breaching a white façade before detonating an explosive to blow open a door the thieves escaped with three golden artifacts: the golden helmet of Coțofenești and three golden bracelets The main suspects in the case are Douglas Chesley W. Both were arrested shortly after the robbery Duin claims that his client had no connection to these suspects "He was shown photos of the individuals linked to the robbery but he didn't recognize any of them," the lawyer said Authorities have yet to release detailed information regarding the suspects' actions and connections The prosecution has stated that they will not comment further until the pre-trial hearing scheduled for May 9 in Assen the court will decide whether three of the seven detained suspects should remain in custody While the primary suspects face the most serious charges remains under investigation for potential complicity Duin stressed that his client was unaware of the criminal intent behind the vehicle’s use "He only earned a small amount — the deposit for the rental car," Duin added "He had no idea what it would ultimately be used for." The court will also assess the continued detention of the remaining suspects as authorities continue to piece together the full scope of the heist Spring brings on the urge to get rid of our stuff Here are sustainable ways to get the job done Read also: | A #CleanTok pro opens up | Cleaning tips from readers Spring is often considered to be one of the best times in the year to reset your home whether deep cleaning it or clearing out your closet But before you start purging clothing or other household items you no longer need it’s worthwhile to think about where your unwanted stuff will go From 2019 and 2020, it is estimated that Canadians produced 12 kilograms of textile waste per person at the household level. This number was derived from measured average textile waste rates in Ontario, based on joint research from Seneca College and the University of Waterloo and from Canadian residential waste disposal rates from 2002 to 2018 Experts say there are plenty of environmentally conscious ways to deal with gently used items without creating more waste Here are some of their tips for how to get rid of your stuff Sheri Pavlović, who goes by Refashionista Sheri offers free tutorials for upcycling clothes and other items on her website She says the first step to decluttering is starting with a small area such as your closet She suggests putting on your favourite songs and trying on clothes to decide if they still fit or are worth keeping ask yourself these questions: Have I ever used this Once you’ve identified the things you want to give away Pavlović advises dividing them into three piles: upcycle The first pile is for items you want to keep but need an update This includes anything that can be transformed and reused to either wear or decorate with Items in good condition go in the sell pile Those you don’t want to keep but also don’t belong in either of the other piles belong in give away/swap/donate Pavlović suggests inviting friends and family over to have a peek through your give away pile and bring their own unwanted items for a swap party a decluttering and downsizing family-owned business in Ottawa that she and her aunt set up in 2012 It partners with local charities and grassroots organizations for donations and van Beilen advises individuals to try to do the same when spring cleaning “I would rather donate to a local charity that helps the community as opposed to a big company making money off of charity,” she said van Beilen recommends contacting charities and asking what types of things they’re looking for – and only giving items that meet their needs She notes that some organizations collect very specific things – such as the Toronto-based Artscan Circle which accepts donations of new and lightly used musical instruments to give to Indigenous youth in remote communities Other spring cleaners may prefer to donate or even sell things they no longer want to people in their neighbourhood managing director of the Nextdoor Canada app says he’s noticed many users looking to get rid of stuff close to home The app has a built-in marketplace called For Sale & Free sell or give away items to people in their area “We’re seeing a real surge of ecoconscious community initiatives on the app where neighbours are organizing to reduce waste and help each other save money,” Doyle said Pavlović also recommends using neighbourhood-focused apps as a way of finding local homes for items you wish to sell or donate It may be harder to figure out what to do with some items that are piling up in our homes van Beilen suggests converting them into digital formats to help reduce clutter stamps and old photographs can also be donated to library archives and historical societies “If people are not concerned about giving their grandmother’s wedding photos away for privacy reasons While donating items can divert them from landfills in Canada experts say much of the clothing Canadians give away still ends up creating waste in other countries “You should go and see the landfills in East Africa with Toronto Maple Leaf shirts We’re exporting our garbage,” said Kate Bahen managing director of Charity Intelligence Canada a non-profit organization that researches charities and advises donors on where to spend their money In 2023, Canada exported US$144-million in used clothing making it the ninth largest exporter of used clothing in the world according to the Observatory of Economic Complexity Destinations for used-clothing exports included Pakistan Bahen echoes the advice of other experts who suggest Canadians focus on donating useful items to local charities She also says it’s best to avoid unlicensed donation bins because clothing dropped there is usually sent to other countries But she notes items that are in poor and damaged conditions should simply be thrown away “We need to put more stuff in the garbage where it goes to a Canadian landfill that has proper environmental controls rather than in garbage dumps around the world.” it all comes down to keeping possessions at a minimum in the first place to avoid waste Report an editorial error Report a technical issue Editorial code of conduct Check your horoscope to learn how the stars align for you today Sharpen your mind with today’s Cryptic Crossword Refine your strategy and solve the Daily Sudoku Relax and engage with the Daily Concise Crossword Authors and topics you follow will be added to your personal news feed in Following Read also: | A #CleanTok pro opens up | Cleaning tips from readers conducting field work and producing a podcast episode on nature-based climate solutions for Farm Radio International Dominique spent three months researching Black Canadian newspapers from the 1850s to now that led to Carleton University’s first course on the history of the Black Press in Canada Dominique has lived in four countries so far and is not planning to settle down anytime soon Tony Keller is a columnist with The Globe and Mail He joined The Globe in 1991 as an editorial writer; over a career of more than 30 years he has also served as editor of The Financial Post Magazine managing editor of Maclean’s and a TV news anchor on BNN (now BNN-Bloomberg) He returned to The Globe in 2013 to become the paper's editorials editor and remained in that position until 2022 he’s a graduate of Duke University and Yale Law School and has also been a visiting fellow at the University of Toronto Faculty of Law and the Wilson Center in Washington D.C He’s been nominated three times for the National Newspaper Award for editorial writing Andrew Willis is a business columnist for the Report on Business Working in business communications and journalism for three decades from 2010 to 2016 he was senior vice-president of communications for Brookfield Asset Management a leading global alternative asset management company which exposed the ways that Canadian police services mishandle sexual assault cases training and practices around sexual violence Doolittle’s other notable projects include the “Power Gap”, an investigation of gender inequities in the workforce, and “Secret Canada,” which examines Canada’s broken freedom of information system She is the author of two books, “Had It Coming – What’s Fair In The Age of #MeToo?,” which was shortlisted for the RBC Taylor Prize for non-fiction, and “Crazy Town: The Rob Ford Story,” both of which were national bestsellers Jameson Berkow is the capital markets reporter for The Globe and Mail to cover the economic implications of cannabis legalization He left in early 2020 to start an entrepreneurship magazine and rejoined The Globe in early 2022 to cover financial regulation and governance for Globe Advisor With more than a decade of experience in financial journalism Jameson was most recently the senior reporter for BNN Bloomberg (formerly the Business News Network) where he led live daily coverage of major business news from the television station’s Toronto headquarters He previously worked as the station’s Western Canada bureau chief based in Calgary where his reporting on pipeline politics and the 2014 oil price crash was nominated for numerous awards.\nHis series of reports from Fort McMurray Alberta in 2015 was a finalist for the RTDNA Dave Rogers Award Jameson was the technology reporter for the Financial Post in Toronto where he created and hosted the FP Tech Desk podcast and authored the weekly Startup Spotlight profile series Jameson got his start in journalism in 2007 as a fact-checker for Toronto Life magazine where his first byline was for a story about two dogs getting married Paul Attfield is a reporter at The Globe and Mail Born in England and raised both there and in France Paul is now a dual citizen of Canada and the United Kingdom He has called Toronto home since moving there from London in 2005 Working in The Globe’s sports department since 2006 Paul started out covering predominantly soccer and rugby he has become more of a general assignment reporter writing about pretty much anything involving a bat Temur Durrani is a national reporter for The Globe and Mail a Globe business podcast about how our failures shape us he was a technology reporter for The Globe’s Report on Business he broke news and wrote extensively about Canadian firms like Shopify turbulence in global cryptocurrency markets A globe-trotting newshound hailing from British Columbia and even the Raptors’ historic run to the NBA final Before joining The Globe in February of 2022 where he reported investigative stories and business features for broadcast and digital audiences he was a staffer at the Winnipeg Free Press A juror since 2021 for the annual Dalton Camp Award which grants young writers with a $10,000 prize for the best essay on the link between media and democracy TV and radio panels to provide news analysis He speaks in six languages fluently or conversationally (guess which ones!) takes his caffeinated beverages very seriously Carrie Tait is a reporter in The Globe and Mail’s Calgary Bureau Her coverage ranges from race relations in her home province of Saskatchewan to the lighthearted topic of skiing cats in Alberta Carrie has reported on the wildfires and floods in Alberta and British Columbia; how Cargill’s meat-processing plant in High River became the site of Canada’s largest single outbreak of COVID-19; and naming trends among Calgary Stampede participants she covered energy for the Globe’s Report on Business and has also reported for the National Post She joined the National Post’s Calgary bureau in 2008 Barry Hertz is the Deputy Arts Editor and Film Editor for The Globe and Mail He previously served as the Executive Producer of Features for the National Post and was a manager and writer at Maclean’s before that Barry’s arts and culture writing has also been featured in several publications, including Reader’s Digest and NOW Magazine. His favourite film franchise is the Fast and Furious series and he will offer no apologies for that fact Conservative Party Leader Pierre Poilievre and his wife Anaida Poilievre depart a polling station after voting in Ottawa on Monday Cathal Kelly is a columnist for The Globe and Mail Emily Haws has been a journalist on Parliament Hill since 2017 except for a stint in Iqaluit with CBC North Emily was a producer for the Cable Public Affairs Channel (CPAC) and CBC's Power & Politics Welcome to The Globe and Mail’s comment community. This is a space where subscribers can engage with each other and Globe staff. Non-subscribers can read and sort comments but will not be able to engage with them in any way. Click here to subscribe If you would like to write a letter to the editor, please forward it to letters@globeandmail.com. 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For more information on our commenting policies and how our community-based moderation works, please read our Community Guidelines and our Terms and Conditions we are discussing the madness that occurred at the Ft Lauderdale Pro Swim Marchand has a few areas of improvement to work on if he hopes to return to his Paris form at the World Championships this summer in Singapore having tight battles in both the 200 and 400 IM but ultimately touching 2nd in both Cal Bear and Mexican swimmer Humberto Najera took down a pair of national records in the men’s backstroke events the oldest swimmer to win a medal at a World Championship would qualify for the 2025 World Aquatics with his record-setting swim October 27th, 2012 International, Short Course World Championships Swimming Canada has announced their lineup for the 2012 World Short Course Championships that will take place from December 12th-16th in Istanbul Like we’ve seen from many countries outside of the European continent the federation is sending very much of a slimmed-down squad to the meet The women’s side will carry a lot more fireworks than the men’s side will on this roster including the country’s top two breaststrokers Martha McCabe and Tera van Beilen Brittany and Heather are also joining this squad off of Canada’s Olympic team; as is butterflier Katerine Savard who was a member of the Canadian 400 free relay team that finished 10th overall It’s also interesting to note the split between swimmers who compete/competed in the NCAA (America’s college system) and the CIS (Canada’s college system) This has become a hot-button issue as Canada pushes to develop their domestic system Brittany MacLean and Chantal van Landeghem This meet will fit well into their schedule as Georgia is already sending a big contingent to the meet Other NCAA swimmers on this roster include Jake Tapp but it is largely a home-grown lineup for Istanbul who will be the first member of the Simon Fraser Canadian/NCAA crossover squad to make an international roster after they’ve begun official NCAA competition The City of Vancouver continues to be Canada’s hotbed for swimming talent as 5 of the 14 members of the team represent the Vancouver Dolphins and over half (8 out of 14) coming from the National Swim Centre there an exciting young freestyler who just missed the Olympic Team Δdocument.getElementById( "ak_js_1" ).setAttribute( "value" Δdocument.getElementById( "ak_js_2" ).setAttribute( "value" More from Braden KeithSee All Subscribe to our newsletter and receive our latest updates! © 2025 Swim Swam Partners, LLC. All rights reserved. Volume 11 - 2020 | https://doi.org/10.3389/fmicb.2020.01503 All Three Endogenous Quinone Species of Escherichia coli Are Involved in Controlling the Activity of the Aerobic/Anaerobic Response Regulator ArcA A Corrigendum on All Three Endogenous Quinone Species of Escherichia coli Are Involved in Controlling the Activity of the Aerobic/Anaerobic Response Regulator ArcA by van Beilen, J. W. A. and Hellingwerf, K. J. (2016). Front. Microbiol. 7:1339. doi: 10.3389/fmicb.2016.01339 In the original article, there was a mistake in the legend for Figure 1 as published There were small discrepancies between the text of this legend and the drawing of the figure In the original article, there was a mistake in Figure 1 as published. In the chemical structure of two intermediates in the biosynthesis of menaquinone a key hydroxy group was missing. The corrected Figure 1 appears below Biosynthesis routes of the quinones of Escherichia coli Dashed arrows indicate enzymes deleted in mutants used in this study The double arrow symbolizes the action of the introduced heterologous MenHBsu The underlined intermediate C1-demethyl-C6-demethoxy-Q8 (DDMQ8) may accumulate in a ubiE mutant strain (for references: see text) Established bio-active quinones are indicated via their abbreviation The authors apologize for these errors and state that this does not change the scientific conclusions of the article in any way Citation: van Beilen JWA and Hellingwerf KJ (2020) Corrigendum: All Three Endogenous Quinone Species of Escherichia coli Are Involved in Controlling the Activity of the Aerobic/Anaerobic Response Regulator ArcA Received: 11 May 2020; Accepted: 10 June 2020; Published: 15 July 2020 Edited and reviewed by: Lars E. P. Dietrich Copyright © 2020 van Beilen and Hellingwerf. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) distribution or reproduction in other forums is permitted provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited in accordance with accepted academic practice distribution or reproduction is permitted which does not comply with these terms *Correspondence: Klaas J. Hellingwerf, ay5qLmhlbGxpbmd3ZXJmQHV2YS5ubA== Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher 94% of researchers rate our articles as excellent or goodLearn more about the work of our research integrity team to safeguard the quality of each article we publish Please select what you would like included for printing: Copy the text below and then paste that into your favorite email application Geesje Marchein “Gaya” – passed away peacefully at Miramichi Lodge on Friday Gaya Vandersleen (nee Van Beilen) of Pembroke dearly loved wife of Jan Vandersleen and by first marriage the late John Vandersleen (October 25 1988).  Dear mother of Gerrit Vandersleen (Ellen) Russell.   Grandmother of 14 grandchildren and 12 great-grandchildren Daughter of the late Jan and Antje Van Beilen (nee Buter).  Sister of Ann Vandemheem (Mac) Luke Van Beilen (late Bea) and Jenny Chamberlain (Brent) Predeceased by brother Jake Van Beilen (Corrie).  2023 from 2-4 and 7-9 p.m.  A Funeral Service will be held at Zion Lutheran Church Pembroke on Saturday at 11:00 a.m.  Spring interment in Wesley United Cemetery donations to Miramichi Lodge Foundation or Zion Lutheran Church would be appreciated Enter your phone number above to have directions sent via text This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply Service map data © OpenStreetMap contributors Lorena Wiebes wins the 2023 Ronde van Drenthe 158.1km route from Beilen to Drijber battled out over laps of the explosive VAM-berg Lorena Wiebes wins Ronde van Drenthe for a record fourth year in a row Hello and welcome to the Ronde van Drenthe 2024 The race today covers 158.1km and takes on six ascents of the famous landfill climb of the VAMberg.  Lorena Wiebes of SD Worx-Protime has taken the last three times on the cobbles This time she faces off against her former leadout woman and main rival The DSM-Firmenich-PostNL star has struggled for form at the start of the year after getting just before the UAE Tour But she has been getting better and better.  Wiebes and Kool have ridden against each other this season at the Omloop van het Hageland where neither rider got near victory.  There are plenty of other riders who can take this win We saw this at the European championships with Mischa Bredewold taking the race solo anyone in the team for that squad could win today.  Others are Chiara Consonni with Sofia Bertizzolo giving UAE Team ADQ options Aniina Ahtosalo and Maria Giulia Confalonieri from Uno-X Mobility with Fenix-Deceuninck having Christina Schweinberger and Puck Pieterse The race has started with the riders doing 2.2km of neutral zone The race s already only 4km from the first time up the VAMberg There is a sprint at the top of the VAMberg This is one of two minor competitions in the race Potentially something for the smaller teams Over the top of the VAMberg and just awaiting the sprint result.  There has been a crash unfortunately for Alex Morrice of Canyon-SRAM.  About the hit the VAMberg for the second time The race had split into three groups but the front two have come back together There is a 28kph win from the east today with the temperature at 11 degrees C.  The 30-year-old has no pro wins to her name but some good results in races like a podium in the Belgian national championships road race as well as GP Vårgårda and a top 10 on the GC in Thüringen Ladies Tour Demey is about 6km from the third ascent of the VAMberg She has 40" on the peloton who have chiseled off 20" since the last gap was given Demey is about the start the VAMberg for the third time with the peloton now +30" down with 90km to go.  Demey has crossed the line with three laps to go and she has a gap of +1'11" over the peloton.  Live pictures show that a small group of riders are out of the back at moment Lidl-Trek and Human Powered Health lead the way.  Demey has 2'20" gap now as the Belgian is doing a brilliant race with 73km to go.  Interesting that Finland's national champion Lily Williams for Human Powered Health is the rider who went down Mischa Bredewold also hit the deck as well as several others thankfully with Williams just straightening her bars before heading off again.  As the race head the the VAMberg for the fourth time the pace in the peloton has really slackened the gap to Demey has fallen to 57" with 62km to go.  Demey's gap is said to be 1'26" as she starts the VAMberg once again.  A mechanical for one rider in the peloton led to a split on the first slopes of the VAMberg Valerie Demey looks to be really hurting on the second kicker to the highest point of the climb.  She has 1'20" on the peloton led by Ahtosalo with 57km to go.  Very frustrating for the American who will now have to wait for the team car which will be a long way down Interesting that riders like Georgia Baker and Maaike Boogaard seemed to be struggling on the VAMberg Two riders you'd usually see up towards the front.  but with one and a half passaged up the VAMberg left they may not be involved at the end.  Before the last ascent of the VAMberg the DSM-Firmenich-PostNL sprinter This surely means that they are working for British champion She managed a top five in the European Championships last year on this climb.  They have really dramatically dropped the pace as various teams spread across the road.  but the AG Insurance-Soudal and Fenix-Deceuninck riders don't even manage a gap.  Fenix-Deceuninck and DSM-Firmenich-PostNL are attempting to create splits in this moderate north-easterly wind but the pace has caused some splits further back in the bunch The peloton has maybe lost a third of it's size in the wind.  Chantal van den Broek-Blaak has been distanced by the peloton in this mini echelons.  Valerie Demey is caught by a flying peloton.  Alice Wood (formally Barnes) on the front alongside another British rider Now SD Worx-Protime hit the front en masse including Wiebes who looks really up or this Multiple teams trying to split the bunch even more.  The peloton are heading to the final full ascent of the VAMberg The finish only takes on the first half.  Audrey Cordon-Ragot and Abi Smith are a couple of riders distanced by the pace at the front of the bunch Also in that dropped group was cyclo-cross star Smith and Cordon-Ragot make it back to the bunch with 33km to go The pace has ebbed somewhat before turning towards the VAMberg again.  Pfeiffer Georgi leads onto the VAMberg for the penultimate time with Mischa Bredewold joining her at the front Puck Pieterse leads over the top and stretches the peloton right out and has caused some splits with six riders going clear!  The remnants of the peloton aren't too far behind They're about 10" back on the leaders.  UAE Team ADQ catch the chasing trio and are closing on the leaders with 16" between the two groups.  The eight leaders are working very well together They have 10" on the peloton with Anniina Ahtosalo trying an attack from the peloton.  Ahtosalo's acceleration is bringing the peloton right back into the race as the gap is shrinking to the leaders with Bertizzolo catching Ahtosalo.  Maria Giulia Confalonieri took it upon herself to bring the leaders back as we have a group of 25 to 30 with 18km to go.  But SD Worx-Protime jumps straight onto that one.  Lily Williams has made it back to the lead of the race a mechanical and a bike change and has managed to get back in.  SD Worx-Protime and Human Powered Health leading the peloton.  The peloton is now around 40 riders strong The pace is quite steady with the teams of Wiebes and Kool doing the pacing.  DSM-Firmenich-PostNL and SD Worx-Protime leading the way with 5km to go.  Human Powered Health squeeze through alongside DSM-Firmenich-PostNL and SD Worx-Protime pinning Lidl-Trek back as it stands.  the pace is rising quite dramatically with the sun setting as dusk closes in.  SD Worx-Protime and DSM-firmenich-PostNL are the two teams once again dominating but Lidl-Trek and UAE Team ADQ finally moving up for their sprinters into the final corner.  Georgi leading with Kool perfectly placed in third wheel as Majerus is moving Wiebes up as the climb starts.  500m to go as Pieterse is moving up but Georgi still leads.  Lorena Wiebes wins the Ronde van Drenthe for the fourth time in a row But she just couldn't live with the detonation of an acceleration by Wiebes as they hit the cobbles in the final 200 metres.  Puck Pieterse takes a brilliant third place for Fenix-Deceuninck.  Keep an eye out on the home page for the race report and quotes from the likes of Lorena Wiebes and others.  all the reaction from the finales of Paris-Nice and Tirreno-Adriatico that took place today.  Plenty more racing to come this season with the first Monument of the season Milano-Sanremo taking place next weekend.  There was a buzz in the air recently at Dunkirk Senior High School as the Large Group Instruction Room was standing room only for the college signing event for Champ Texidor and Sebastian DeGolier school administration and fellow classmates A welcome was provided by school administration and former Dunkirk football coach Mike Sarratori spoke on behalf of both the young men Texidor and DeGolier took signing photos with family Champ is the son of Dorothy Beilen and Samuel Texidor Champ will be attending Morrisville State University of New York Champ wanted to express his gratitude for those who helped him be successful during this journey “I want to give a huge thank you to my friends and especially the teachers at Dunkirk that have helped me come this far,” he said Sebastian is the son of Jayson and Amanda DeGolier Sebastian will be attending Utica University playing football as an offensive lineman and majoring in sports management Sebastian wanted to select a university that makes him feel comfortable “Picking a place to be for the next four years of your life will be one of the most important decisions you make so you want to make sure the place you choose will feel like a home,” he said “That’s how I feel with Utica.” “Sebastian and Champ are perfect examples of the types of kids Dunkirk athletics Both are respectful young men with a team first mentality,” Sarratori said “Sebastian and Champ have set goals in and out of athletics and have committed themselves to reaching those goals This accomplishment has been an accumulation of all the sacrifices these student/athletes and their families have made I am honored to have been able to coach Sebastian and Champ and I look forward to watching them FREDONIA — Danielle Palisin tossed a three-hitter with eight strikeouts and did not walk a batter as Fredonia .. Fredonia put together a four-run fifth inning and beat Salamanca 8-3 through a driving .. BUSTI — Fredonia’s Sydnee Hoffman shot a 24 over five holes but Southwestern beat the Hillbillies 35-20 in .. Copyright © 2025 Ogden Newspapers of New York | https://www.observertoday.com | PO Box 391 This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page 8x Olympic medalist Regan Smith graces the cover of SwimSwam Magazine’s 2025 Women In Swimming Issue Ilya Kharun opted out of both of his B-final berths at the Pro Swim Series while many other stars scratched one event in favor of another and Judy Garay combined for five Masters short-course meters world records at the 2025 Ontario Masters Swimming Championships The Board wants to eliminate a $58 million budget deficit is considering closing pools it doesn’t lease out and cutting the jobs of 86 aquatic instructors lowered her own national record and came within .01 of the US Open record in prelims of the Fort Lauderdale Pro Series February 18th, 2015 Canada, College, International, News To stay on top of our coverage of the CIS this weekend, bookmark the page for our Canadian channel here NICKNAME/ FUN FACT: “My nickname has always been TVB impersonating people and telling stories.” UBC FUN FACT/ FAVOURITE CIS MEMORY: “For lunch one day we had chili and let’s just say we blame everything on the chili now.” Canadian female swimming has typically had some top notch breaststrokers and fitting right into that category is UBC’s Tera Van Beilen has had plenty of success in the breaststroke events and is a favourite to make the World Championships roster this summer Van Beilen made the semi-finals in the 100m breaststroke at the 2012 Olympic Games where she tied for eighth with Jamaican Olympian Alia Atkinson Van Beilen finished second in the swim-off taking a ninth place finish overall Van Beilen competed at the Commonwealth Games and Pan Pacific Championships picking up a bronze medal in Glasgow as part of the 4x100m medley relay team Van Beilen is one of Canada’s top competitors on the international scene and one of the most dominant breaststrokes overall in the CIS She’ll be swimming all three breaststroke events as well as the 400m IM this year and will have stiff competition in all of those races her biggest CIS rival in the breaststrokes has been Calgary’s Fiona Doyle “Fiona and I have had fun over the years battling it out in all the breaststroke races It’s great to have competition like that within the CIS and that battle will definitely continue over this coming weekend,” said Van Beilen The long course finals shouldn’t change the competitiveness of the races one bit as both are international competitors and used to competing in the longer pool however stated that she did enjoy the short course format of the CIS Championships leading up to trials As one of the favourites to make the Worlds team Van Beilen is using this meet as preparation for Trials which will take place in Toronto starting April first The new long course format of the championships has given her a chance to train more long course as the meet approached “We’ve been doing more back-to-back practices long course instead of doing our race set-up short short course we did it long course.” As for special preparation Van Beilen included UBC’s Valentines Day plans “we also baked cupcakes and wrote poems for the guys team on Valentines day and they gave us roses and chocolates.” The team camaraderie  at UBC is one of the reasons that they’ve been so successful The women are going into the meet looking for their fourth straight title something that they have only done once before during the decade of dominance “Our team has grown so strong as a cohesive group over the last couple months and I think we have the depth and talent to do amazing things this weekend,” said Van Beilen “The UBC women’s swim team is like a second family to me and the incredible experiences we’ve shared throughout the years are some that I will remember for a lifetime.” Van Beilen has played a major role as a leader on the UBC roster and although her talent stretches far beyond the CIS she’s still constantly trying new things such as new events and having fun with university swimming “I haven’t ever raced the 400m IM long course with some rest so that will be a fun race.” As soon as the CIS Championships are finished Van Beilen will be heading to back to UBC to prepare for trials She believes that this weekend will be a great opportunity to see where she’s at in her preparation it will be a good opportunity to get some solid short and long course racing in before trials We use this meet every year as a preparation for trials.” “should be some more great racing in just over a months time.” More from Mitch BowmileSee All Volume 4 - 2013 | https://doi.org/10.3389/fmicb.2013.00157 This article is part of the Research TopicMicrobial mechanisms of tolerance to weak acid stressView all 11 articles The internal pH (pHi) of a living cell is one of its most important physiological parameters To monitor the pH inside Bacillus subtilis during various stages of its life cycle we constructed an improved version (IpHluorin) of the ratiometric pH-sensitive fluorescent protein pHluorin by extending it at the 5′ end with the first 24 bp of comGA which showed an approximate 40% increase in fluorescence intensity was expressed from developmental phase-specific subtilis that are specifically active during vegetative growth on glucose (PptsG) or during sporulation (PspoIIA compartment-specific expression of IpHluorin that allowed accurate pHi measurements of live cultures during exponential growth Dormant spores were characterized by an pHi of 6.0 ± 0.3 Upon full germination the pHi rose dependent on the medium to 7.0–7.4 The presence of sorbic acid in the germination medium inhibited a rise in the intracellular pH of germinating spores and inhibited germination Such effects were absent when acetic was added at identical concentrations as well as an indicator of the metabolic and energetic state of the cell To gain further insight in the putative pHi dynamics of these differentiation processes we studied the pHi of the mother cell and fore-spore independently During sporulation the development of the pHi in the mother cell and the nascent fore-spore may also give insight in the level of independence of the two cells It is assumed that the accumulated 3-PGA serves as an initial carbon and energy source for the cell Expression of IpHluorin resulted in strong This allowed us to monitor the pHi during growth and sporulation Effects of the addition of sorbic and acetic acid on the pHi of germinating spores are described subtilis were prepared by glucose depletion of defined liquid medium (M3S Cultures were incubated for 4 days at 37°C under continuous agitation (200 rpm) Spores were harvested and purified by extensive washing with MilliQ water at 4°C The spore crops were inspected by phase-contrast microscopy and were free (>99%) of vegetative cells Spores were stored for up to 1 week in MilliQ water at 4°C at optical density (OD)600 = 1 This SD-improved pHluorin (IpHluorin) was inserted between the HindIII and SalI sites of pDG148 subtilis PB2 and compared with PB2 carrying pDG-pHluorin to analyze expression levels and pH-dependent characteristics of pHluorin and IpHluorin genome-integrated expression system was constructed thereby replacing GFP and placing IpHluorin under control of the xylose-inducible Pxyl promoter The promoter and SD-IpHluorin sequences were fused by a PCR and inserted in pSG1729 thereby replacing the GFP and placing IpHluorin under control of a B All enzymes used were obtained from Fermentas (Thermo Fisher Scientific) Bacillus subtilis PB2 was used as target for our transformations. B. subtilis cells were made transformation-competent as described before (Kunst and Rapoport, 1995). The newly constructed plasmids were integrated in the amyE locus as described (Lewis and Marston, 1999). All plasmids and oligonucleotides used in this study are listed in Tables 2 and 3 Bacillus subtilis PB2 containing either pDG148 pDG-pHluorin or pDG-IpHluorin were grown to exponential phase in M3G at pH 7.0 containing 10 μg/ml kanamycin Bacterial growth and expression levels of ratiometric pHluorin and IpHluorin were monitored in a FluoStar Optima (BMG Labtech Germany) for 3 h after addition of 0–1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) Fluorescence and OD600 were monitored for 30 min Calibration curves for pHluorin and IpHluorin were identical with only minor fluctuations in fluorescence in time observed with pHluorin at pH = 8.5 we concluded that the intracellular and extracellular pH had equilibrated rapidly subtilis PB2 carrying pDG148 was measured for background fluorescence Background fluorescence was subtracted at individual wavelengths before calculating the ratio The calibration curve was determined by fitting the data of three independent biological replicates each consisting of three technical replicates with a polynomial curve of the third order Microtiter plates were placed in a FluoStar Optima (BMG Labtech Germany) at 37°C and shaken between measurements (200 rpm) with pH and OD600 measurements taken every 10 min To verify if expression of IpHluorin was correctly localized, B. subtilis cells were cultured as described above for batch measurements at pH = 7.0. All strains were grown as described to exponential phase or for 16–24 h to observe sporulating cells. Cells were immobilized on 1% agarose (Koppelman et al., 2004) and photographed with a CoolSnap fx (Photometrics) charge-coupled device (CCD) camera mounted on an Olympus BX-60 fluorescence microscope through an UPLANFl 100×/1.3 oil objective (Japan) with a 41017 - Endow GFP/EGFP Bandpass filter (Chroma Technology Corp. (A) Schematic overview of the improved pHluorin construct SD – Shine–Dalgarno sequence (B) Expression levels of pHluorin and improved pHluorin after induction with IPTG arbitrary units) at 510 nm from excitation at 390 nm was measured after 2.5 h of induction and normalized to OD600 Error bars indicate standard deviations (n = 3) Both growth rate and the ratiometric characteristics were similar and allowed reliable pHi readings in a range between pH 5 and 8.5 in live B Left panels: phase-contrast images; right panels: corresponding fluorescent signals (A,B) Non-transformed cells; (C,D) PptsG-IpHluorin-expressing cells; (E,F) sporulating cells expressing PspoIIID-IpHluorin; (G,H) sporulating cells expressing PB2 PsspE-IpHluorin Representative example of the expression levels of IpHluorin from different promoters during different growth stages (A) Fluorescence levels from excitation at 390 nm and emission at 510 nm from different promoters used Expression of IpHluorin from sporulation-specific promoters starts only after the drop in OD600 Internal pH as measured with IpHluorin expressed from different promoters The pH is calculated from when the 390 nm channel exceeded 1,000 units pH from the spore-specific promoters is calculated from approximately 17 h onward Lines represent actual data from a representative example We do not know from which cells this signal originates as it may represent the average of various differentiation types a cut-off of 1,000 arbitrary units in the 390 to 510 nm fluorescence channel was used for pHi calculations SpoIIA is activated by high levels of activated Spo0A and its presence was shown to be a reliable indicator for cells that initiate sporulation (Veening et al., 2005). Indeed, a fluorescent signal of a GFP reporter under control of the SpoIIA promoter can be found in both mother cell and fore-spore (our unpublished observations; Veening et al., 2006b) Expression of genes in the mother cell regulated by PspoIIID follows that of those regulated by PspoIIA but because expression levels of PspoIIID-controlled IpHluorin are higher reliable pHi measurements can be obtained earlier with the latter the sporulating subpopulation had a pHi that closely resembled the pHi of exponentially growing cells measured with the PptsG-IpHluorin strain The mother cell (PspoIIID-IpHluorin) had a pH of 7.8 after 17 h of incubation IpHluorin expressed from the spore-specific promoter PsspE revealed pH values of 7.4 The mother cell-specific expression of IpHluorin from the spoIIID promoter decreased after 25 h of culture an apparent decrease measured with the mother cell-specific promoter driving IpHluorin expression was observed may at least partially be influenced by mother cell lysis and release of IpHluorin into the medium The inferred pH at 40 h of culture closely resembled medium pH The decrease in pHi in the fore-spore (PsspE-IpHluorin) drops below the medium pH and its fluorescent signal can clearly be observed inside maturing spores (Figure 2H). Noticeably, spores have a very low water activity and optical properties dissimilar from vegetative cells, which may obscure the pH as defined as the number of free protons (Sunde et al., 2009) Internal pH during spore germination and outgrowth IpHluorin accumulated in the spores (from expression controlled by PsspE) allows pH measurements from t = 0 to approximately 300 min Expression of IpHluorin from PptsG allows calculation of the pHi from approximately 200 min Data are from a representative example of germination and outgrowth of B consisting of phase-bright and germinating spores the actual pH change in individual germinating spores may differ Germination with medium pH = 7.4 shows a more rapid decrease in OD600 and an equally faster rise in pHi but follows a similar trend as with medium at pH = 6.4 Dormant spores are highly resistant to antimicrobial treatment, but also metabolically inert (Brul and Coote, 1999). When germination is triggered, the spore becomes more sensitive. Also, it has been observed that germination of Bacillus spores can be inhibited by various preservatives (Cortezzo et al., 2004; Van Melis et al., 2011) during this stage water is taken up and metabolism should be restarted These processes might be a target moment for WOAs to halt outgrowth of the germinating spore subtilis spores at an external pH = 6.4 with 3 mM KS subtilis spores at an external pH = 6.4 with 10 mM KS subtilis spores at an external pH = 6.4 with 25 mM KAc subtilis spores at an external pH = 6.4 with 80 mM KAc OD600 and internal pH during germination of B subtilis PB2 PsspE-IpHluorin spores in medium without glucose (pHo = 6.4) (A) Germination with AGFK; (B) germination with AGFK in 0.5 mM sorbic acid; (C) germination with AGFK in 0.5 mM acetic acid We show here that IpHluorin is an accurate versatile probe to investigate the pHi of B We were able to improve expression of pHluorin by fusion of the first 24 bp of comGA with the pHluorin-encoding gene Genomic integration of IpHluorin resulted in more homogeneous expression levels compared to a multi-copy plasmid It also resulted in a more stable construct not requiring antibiotics for maintenance of the IpHluorin gene during extended periods of growth (not shown) The use of genomically integrated constructs with endogenous promoters for the expression of IpHluorin resulted in a strong enough signal for accurate pH measurements during exponential growth on glucose as well as compartment-specific pHi measurements during sporulation The IpHluorin that accumulates in the spore under control of PsspE allows pHi measurements of the B expressed from PsspE overlaps slightly in time with PptsG-IpHluorin expression thus allowing continuous pHi monitoring during germination and outgrowth in batch The pH values we have observed here closely resemble those found with other methods subtilis spores was also found to lie at approximately pH = 6 Despite the fact that expression levels of IpHluorin are much lower in spores the pH value observed again closely corresponds to earlier reported values The notion that during outgrowth a pH is observed that closely resembles the pHi during exponential growth (as observed with PptsG-IpHluorin) further corroborates the accuracy of our method Other methods to measure pHi generally involve compounds that are hydrophobic and have WOA groups and may act as uncouplers thereby depleting the ΔpH and influencing ΔΨ over the membrane They are also more labor-intensive when high temporal resolution is required and except for fluorescent dyes do not allow cell type-specific pH measurements these methods require long-term incubation with the dye plus extensive washing Future studies will have to determine the phototoxicity and bleach rate of IpHluorin in individual (growing so our results show the average of a germinating population During spore germination, the pHi increases due to release of protons (Swerdlow et al., 1981). This process follows the drop in OD600, and results from H2O uptake and release of DPA. Our results show that a ΔpH is established rapidly. Such an increased pH can reactivate PGM, thus allowing the utilization of the spore’s 3-PGA store (Magill et al., 1994) long-term pHi monitoring in growing and sporulating B subtilis cultures as well as during spore germination The pHi of sporulating cells is as high as that of exponentially growing cells This is particularly the case for the mother cell The PptsG-IpHluorin strain can be used for many experiments where the pHi needs to be measured in cells growing on glucose without the need for additional inducers like IPTG The sporulation-specific IpHluorin-expressing strains may give more insight in compartmentalization during sporulation while the PsspE-IpHluorin strain may also help understanding spore germination characteristics in the presence of potential outgrowth inhibitors such as the WOAs sorbic acid and acetic acid Clearly, because not all cells are in exactly the same state, these data represent the average value of the pHi in the population studies. To analyze the heterogeneity single-spore pHi measurements are needed. Currently we are extending our single cell live imaging tool “SporeTracker” (Pandey et al., 2013) to that end The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest Tanneke den Blaauwen and Jolanda Verheul are thanked for usage of and assistance with the microscopy facilities of the department of Molecular Cytology at the University of Amsterdam Frans Klis and Gertien Smits are acknowledged for critically reading initial versions of the manuscript Alex Ter Beek and Janneke Wijman are acknowledged for initial experiments with pHluorin and many stimulating discussions on Bacillus subtilis acid stress physiology Measurement of the internal pH of yeast spores by 31P nuclear magnetic resonance Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Pubmed Abstract | Pubmed Full Text pBaSysBioII: an integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene expression in Bacillus subtilis Pubmed Abstract | Pubmed Full Text | CrossRef Full Text A novel method for continuous determination of the intracellular pH in bacteria with the internally conjugated fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester Pubmed Abstract | Pubmed Full Text Mode of action and microbial resistance mechanisms Pubmed Abstract | Pubmed Full Text | CrossRef Full Text adenylate energy charge and phosphorylation potential to the specific growth rate and efficiency of energy transduction in Bacillus licheniformis under aerobic growth conditions Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Analysis of gene control signals by DNA fusion and cloning in Escherichia coli Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Analysis of the action of compounds that inhibit the germination of spores of Bacillus species Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Requirement of prorenin receptor and vacuolar H+-ATPase-mediated acidification for Wnt signaling Pubmed Abstract | Pubmed Full Text | CrossRef Full Text The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Hydrogen ion buffers for biological research Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Compartmentalization of gene expression during Bacillus subtilis spore formation Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Rapid orientated cloning of a shuttle vector allowing modulated gene expression in Bacillus subtilis Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Analysis of temporal gene expression during Bacillus subtilis spore germination and outgrowth 3624–3634 doi: 10.1128/JB.01736-06 Pubmed Abstract | Pubmed Full Text | CrossRef Full Text R174 of Escherichia coli FtsZ is involved in membrane interaction and protofilament bundling Pubmed Abstract | Pubmed Full Text | CrossRef Full Text pH homeostasis and ATP synthesis: studies of two processes that necessitate inward proton translocation in extremely alkaliphilic Bacillus species Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Molecular aspects of bacterial pH sensing and homeostasis Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Salt stress is an environmental signal affecting degradative enzyme synthesis in Bacillus subtilis Pubmed Abstract | Pubmed Full Text Effects of hydration on molecular mobility in phase-bright Bacillus subtilis spores Pubmed Abstract | Pubmed Full Text GFP vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilis Pubmed Abstract | Pubmed Full Text | CrossRef Full Text The internal pH of the forespore compartment of Bacillus megaterium decreases by about 1 pH unit during sporulation Pubmed Abstract | Pubmed Full Text Analysis of the relationship between the decrease in pH and accumulation of 3-phosphoglyceric acid in developing forespores of Bacillus species Pubmed Abstract | Pubmed Full Text Miesenböck Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins Pubmed Abstract | Pubmed Full Text | CrossRef Full Text On the Intracellular pH of Baker’s Yeast Intracellular pH is a tightly controlled signal in yeast Pubmed Abstract | Pubmed Full Text | CrossRef Full Text In vivo measurement of cytosolic and mitochondrial pH using a pH-sensitive GFP derivative in Saccharomyces cerevisiae reveals a relation between intracellular pH and growth Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Regulation of noise in the expression of a single gene Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Intracellular pH and membrane potential as regulators in the prokaryotic cell Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Live cell imaging of germination and outgrowth of individual Bacillus subtilis spores; the effect of heat stress quantitatively analyzed with SporeTracker Pubmed Abstract | Pubmed Full Text | CrossRef Full Text The effects of fermentation acids on bacterial growth Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Measurements of the pH within dormant and germinated bacterial spores Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Quantitative measurements of proton motive force and motility in Bacillus subtilis Pubmed Abstract | Pubmed Full Text Levels of small molecules and enzymes in the mother cell compartment and the forespore of sporulating Bacillus megaterium Pubmed Abstract | Pubmed Full Text Cytoplasmic pH measurement and homeostasis in bacteria and archaea Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Modelling the effect of sub(lethal) heat treatment of Bacillus subtilis spores on germination rate and outgrowth to exponentially growing vegetative cells Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Processing of a sporulation sigma factor in Bacillus subtilis: how morphological structure could control gene expression Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Inhibition of spoilage mould conidia by acetic acid and sorbic acid involves different modes of action requiring modification of the classical weak-acid theory Pubmed Abstract | Pubmed Full Text | CrossRef Full Text The physical state of water in bacterial spores Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Levels of H+ and other monovalent cations in dormant and germinating spores of Bacillus megaterium Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Weak Organic Acid Stress in Bacillus subtilis To kill or not to kill Bacilli: opportunities for food biotechnology Pubmed Abstract | Pubmed Full Text | CrossRef Full Text 31P nuclear magnetic resonance studies of bioenergetics and glycolysis in anaerobic Escherichia coli cells Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Quantitative analysis of the modes of growth inhibition by weak organic acids in yeast Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Impact of sorbic acid on germinant receptor-dependent and -independent germination pathways in Bacillus cereus Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Visualization of differential gene expression by improved cyan fluorescent protein and yellow fluorescent protein production in Bacillus subtilis Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Phosphatases modulate the bistable sporulation gene expression pattern in Bacillus subtilis Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Single cell analysis of gene expression patterns of competence development and initiation of sporulation in Bacillus subtilis grown on chemically defined media Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Effects of phosphorelay perturbations on architecture sporulation and spore resistance in biofilms of Bacillus subtilis Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Bet-hedging and epigenetic inheritance in bacterial cell development Pubmed Abstract | Pubmed Full Text | CrossRef Full Text and other factors on the analysis of the thermodynamic feasibility of metabolic pathways Pubmed Abstract | Pubmed Full Text | CrossRef Full Text The forespore line of gene expression in Bacillus subtilis Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Initiation of the germination of Bacillus subtilis spores by a combination of compounds in place of L-alanine Pubmed Abstract | Pubmed Full Text Phosphatidic acid is a pH biosensor that links membrane biogenesis to metabolism Pubmed Abstract | Pubmed Full Text | CrossRef Full Text Citation: van Beilen JWA and Brul S (2013) Compartment-specific pH monitoring in Bacillus subtilis using fluorescent sensor proteins: a tool to analyze the antibacterial effect of weak organic acids. Front. Microbiol. 4:157. doi: 10.3389/fmicb.2013.00157 Copyright: © 2013 van Beilen and Brul. This is an open-access article distributed under the terms of the Creative Commons Attribution License distribution and reproduction in other forums provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc *Correspondence: Stanley Brul, Department of Molecular Microbial Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, Netherlands e-mail:cy5icnVsQHV2YS5ubA== Words by Erin Assman and Tera Van Beilen Erin Assman and Tera Van Beilen are both fifth year UBC students and Thunderbirds who will be graduating this spring. Erin just competed in her last swim meet and Tera is looking to compete in her second Olympic games this summer in Rio If you told us we would go to the same university If you told us we would be roommates and best friends Swimming is a small world — you know most of the people you race against We raced against each other basically our whole lives one of us just narrowly beating out the other for the win we started swimming in the same group for the Oakville Aquatic Club in Oakville Club swimming means your group is your built-in friends Christmas and just about every other major holiday and occasion with them We moved through high school and began to find our own voices It would be polite to say that we weren't the fondest of each other From locker room banter to our individual competitive desire for attention We were both great swimmers and could have chosen almost any school in the United States with a full ride we both made the bold decision to stay in Canada and coincidentally both choose to attend UBC and become a beloved Thunderbird How will their UBC teammates choose sides?” “How is Vancouver going to handle these two big personalities?” “Are you SURE that’s the best decision for you?” We replied to these questions with slight hesitation not sure ourselves how exactly this new arrangement was going to work out September of our rookie year hit — we were cordial It was always nice to see a familiar face in passing but honestly we had no desire to actually hang out The occasional dinner with our fellow rookie — Rebecca Terejko — at the Vanier Caf was about as close as we got how did they not see each other 10 times a week?” We had different training schedules since Tera was training towards the Olympics at the National Training Centre and Erin was training with the varsity team This was the kind of setup that we had hoped for when we originally both accepted our offer to UBC Our first year flew by and we were then left with the decision of who we were going to live with for our second year Our original living arrangements fell through and as life sometimes goes we were forced to make the best out of a “bad” situation Tera Van Beilen and Erin Assman of Oakville Ontario make the conscious decision to live together It was literally the day before we had to have our roommate preferences chosen To say I was stressed would be an understatement It wasn't that I didn't want to meet new people When you're training 30 hours a week for an extremely demanding sport you don't exactly want your roommate to be having a party until 2 a.m Rebecca and Heather MacLean were planning on living together They were the perfect fit for each other — all very focused on swimming not huge partiers and they all loved peanut butter I gathered the courage to Facebook message the three of them and make the request to live with them They knew the history between Tera and I and they really just couldn't see how this living arrangement could ever work out for the best I think they had visions of us getting into a huge fight ending in strangling each other over whose cookies were better Side note: we both make freaking fantastic cookies We moved into our apartment in Gage Towers about a month after that fateful day and began to realize that this arrangement could really work Tera came up with our infamous name of “Core Four” — let's just say it involved a little alcohol a parking lot rooftop and the most wonderful day of the year I wasn't the most fond of the name to start — I thought it was kind of cheesy everyone knew us as Core Four — our coaches one of our teammates even got shirts made for us with “Core Four” splashed across the front and #nailedit on the back Tera did make the Olympics she was training for in first year This was part of the reason why I was originally so nervous about living with the two of them you have to be in order to be on the UBC’s swim team— but I’m not an Olympic-level athlete It gives me a little more freedom to do other things that are important to me like being social (athlete-only events are the bomb) about coming home late while everyone else was asleep about not being as smart as the other girls and most of all I was afraid of not being fast enough to be friends and roommates with these outstanding girls and I'm sure Core Four would think I was out of my mind in saying these things That is what's the best part about living with these girls That is something that will never change no matter where we live The fact that my past frenemy became my roommate was an idea that I never thought would become reality Core Four consisted of four very different personalities but we all embraced it each person for who they were This is why I think we worked so well together I was known for the weird creations I made in the kitchen always leaving the cupboards open and constantly have a good story to share or having a ridiculous story told about me I wouldn’t consider myself the cleanest member of Core Four I’m sure I wasn’t always the easiest person to live with is deathly allergic to peanut butter (which happens to be my favourite food) would daily leave her frying pan on the stove and has always been more mature than me These were some of the things that drove me crazy about Erin but somehow became the things that I love about her Something we learned very quickly was how to pick our battles Passive-aggressive dry erase board notes or harsh text messages were not the answer you quickly learn how to better conduct yourself and get along with someone who may very well be your opposite Erin is one of the best listeners I know and I don’t think that any of our tiffs lasted longer than a couple hours because we able to communicate we challenged one another because we really have wanted the best for each other Every time we saw one another we would ask “How was your practice?” “Did you have a good day?” “Whatcha making for dinner?” Our tiring days would often catch up to us but praise the Lord for the our three favourite cable channels — TLC We wouldn’t have survived the week without them along with our unlimited stashes of chocolate chips And then there were the times that all four of us would congregate in our living room and catch up on each others lives From tears of sadness that somehow always turned into tears of laughter we could easily spend several hours chatting before looking at the clock and seeing 10:00 p.m That’s when you know you’re living in a swimmer house Caring for someone takes time — friendship and love doesn’t happen instantly Good friends evolve with you and thanks to Erin we have seen each other grow into women that we are proud of She became the much needed “mother of the household.” She has always kept me accountable for my actions made me feel accepted and valued for who I am and has become my “go-to” person for advice along with supporting me in all my crazy endeavours Have feedback? Email feedback@ubyssey.ca Thanks for visiting The use of software that blocks ads hinders our ability to serve you the content you came here to enjoy We ask that you consider turning off your ad blocker so we can deliver you the best experience possible while you are here Instead of adding resistance to your stroke Anti-Paddles create a slip through the water forcing swimmers to focus on a full arm catch and proper leverage and gear faster than most people cycle through laundry When it comes to swim gear—what really matters is what happens after the unboxing December 29th, 2013 Gear, Lifestyle, Training in your locker; one never knows what kind of nasty diseases they might pick up from hanging around with those pull buoys you’re expected to put it in your mouth and filter your air through it for a rousing set of 100’s head-down butterfly Today’s swimming #swimhack comes from Canadian National Teamer Tera van Beilen (@teravb on Twitter) You can cook your salmon in the dishwasher too But we wouldn’t recommend doing both at the same time) While surely FINIS* would never guarantee the integrity of such a rudimentary cleaning method their products are fantastically produced and will easily hold up Swimmers have been cleaning their snorkels this way since Dean Garraffa first received a patent for his center-mount snorkel in 1996 which would eventually become the FINIS snorkle we’d recommend taking that out first Thanks to Tera van Beilen for sharing this #swimhack with us Full disclosure: FINIS is a SwimSwam partner Subscribe to our newsletter and receive our latest updates May 02nd, 2013 Canada, International, News, World University Games Swimming Canada has named their squad for the 2013 World University Games that will travel to Kazaan Russia this summer to compete against the world’s best students of higher education These rosters were chosen on combination of the CIS Championship results (Canadian college) in combination with the results from the World Championship Trials As was noted in the official Canadian qualifying standards athletes who were eligible for both the World University Games and World Championship teams were encouraged to compete for both Those swimmers who have chosen to do so will swim at the World University games in Kazaan from July 10th-17th and then will have just shy of two weeks to prep for the World Championships in Barcelona Among the biggest names to have accepted that challenge include Brittany MacLean who trains in the United States at the University of Georgia; UBC’s Savannah King who also excels in the mid-to-distance freestyles who has taken over the reigns of the women’s breaststroke group in Canada the road warriors will be NCAA All-American Richard Funk from the University of Michigan William Brothers and Alec Page from the University of Victoria plus UBC’s Coleman Allen and Tommy Gossland They will be joined with names like Katie Caldwell from McGill the sister of Hilary who starred on the 2011 WUG’s team; Michigan freshman Marni Oldershaw; Texas A&M freshman Ashley McGregor; Indiana freshman Brooklyn Snodgrass; and record-breaking sprint backstroker Kelly Aspinall on the men’s team The team is composed of 38 swimmers total; among them Swimming has been Canada’s most successful sport at the Summer Universiade (as the meet is known in the most of the world) Canada has also named the swimmers who will go on their annual European Mare Nostrum tour which this year specifically will include for the Canadian swimmers stops in Barcelona and Canet and Jeffrey Swanston will make up that team Rumor has it that Canadians have to pay their own way to WUG’s Yup the athletes do have to cover the cost according to the list there are three athletes from the CEGEP system November 22nd, 2013 Canada, News The finals at the short course meters Canada Cup in Toronto this evening proved how well the Canadians are swimming in-season this year Savannah King started off the meet by taking the 800 freestyle title in 8:35.45 but was possibly saving some energy for her 200m freestyle final which she swam roughly 10 minutes after completing her 800 King swam a 2:00.48 to finish sixth in the 200 freestyle 17 year old Gators swimmervKennedy Goss taking the gold in 1:58.91 Olympian Barbara Jardin who swam out of lane three in the B final however came home with the fastest swim of the night Four men dipped under the 50 second barrier tonight in the 100 freestyle; Evan Van Moerkerke from the University of Guelph took the title in a 49.46 Van Moerkerke out-touched many time CIS winner Coleman Allen to the wall to take the victory Allen touched in at 49.47 just 0.01 seconds behind him Manning swam the 50m breaststroke shortly after and despite being the top seed going into the meet The women’s 100m breaststroke final boasted two 2012 Olympic breaststrokers: Martha McCabe and Tera Van Beilen McCabe won the event with ease over the field McCabe was just under a second away from her best time of 1:05.84 from a World Cup event this summer McCabe who is known for her 200m breaststroke is showing some great in-season speed that should help her later on in the season Van Beilen finished second behind McCabe in 1:07.54 University of Calgary freshman Tristan Cote won the men’s 400 IM in a personal best time of 4:17.76 White recently committed to swim for the Michigan Wolverines next season One of the most anticipated swims of the meet: the 100m butterfly was just as exciting as predicted Rivals Katerine Savard and Noemie Thomas fought for the entire race Savard just out-touching Thomas towards the finish 56.99 to 57.03 Canadian butterfly veteran Audrey Lacroix came third in 58.78 Thomas and Savard faced off again in the women’s 50m freestyle final Thomas got the upper-hand on Savard in the splash-and-dash Savard finished in seventh while Ariane Mainville won the event in 25.24 The men’s 100m backstroke was won by Russell Wood Wood touched the wall in 52.97 to win the gold young swimmers Emma Ball and Danielle Hanus dominated the race Ball touched in 28.28 behind Hanus’ 28.23 World Championship team member Zach Chetrat completely dominated the field in the 200 fly Chetrat won the event with a time of 1:55.67 and finished over a body length ahead of second place finisher David Sharpe Frank Despond and Robert Wise battled it out until the end in the men’s 400m freestyle and ended up touching the wall with an identical time The dead-heat-final had Wise and Despond both touching in at 3:47.31 the bronze going to 400 IM winner Tristan Cote Second place finisher in the 100m freestyle Coleman touched the wall in 54.60 ahead of second place finisher Evan White The Canadians have shown some fast in-season swimming during day 1 finals day 2 should also be packed with exciting races For full meet results click here CAPTION: Alia (left) is being congratulated by Canadian Tera Van Beilen after winning their 100m breaststroke swim-off at the Aquatics Centre in Olympic Park Atkinson and Van Beilen had tied for fourth in their semi-final one hour and 40 minutes earlier and had to face-off against each other after the final event last night to determine who would get a lane in today's 8:15 p.m The Jamaican posted a national record 1:06.79 while Van Beilen touched the wall in 1:07.73 View the discussion thread. Thanks for visiting Sign In Subscribe Now John's family immigrated to Canada in April of 1950 he decided to pursue work on the West Coast and eventually settled in the community of Agassiz where he met and married his sweetheart of 51 years Sylvia Lanting Dept he was employed with the Agassiz Research Station where he was head of maintenance During these years he furthered his education in the Electrical His 23 years at the Research Station were full of enjoyment with his work family In 1996 he took early retirement and took courses in picture framing and started a new venture of self employment John was Agassiz's well known handyman who could be seen biking to and from fix it jobs there was no job too big or small for John He went on many disaster relief missions with World Renew to North Dakota North Carolina and Texas to help provide housing to those in need Faith and family were paramount in John's life He was the 4th of 10 siblings Jake (Connie) He is also survived by his wife Sylvia (Lanting) and son Luke (Tanya) and three grandsons Ty Brady and Fynn who were the joy of his life Thank you to family and friends who cared for and supported John and his family through the last difficult years A special thank you to the loving staff at Cheam Village and all who were involved in John's care your kindness and patience will not ever be forgotten A Memorial will be held on Saturday Sept 1 In lieu of flowers donations can be made to World Renew Disaster Relief or the Kent Harrison Foundation Dialogue and debate are integral to a free society and we welcome and encourage you to share your views on the issues of the day. 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To learn about our commenting policies and how our community-based moderation works, please read our Community Guidelines 16 August, 2023 By Offshore wind energy services business Venterra Group has bought Dutch offshore piling equipment manufacturer Cape Holland Beilen headquartered Cape Holland develops unique Vibro Lifting Tools to handle and drive piled foundations into the seafloor Its specialist equipment is said to speed up installation using less vessel time protect against “pile runs” and create less noise on the sea’s surface and within the water column Venterra Group has said that Cape Holland’s expertise in low-noise vibratory pile installation will be “a great asset to Venterra and complements the existing foundation installation expertise across the group” Cape Holland was founded in 2002 by the father of the present chief executive officer and its fleet of Vibro Lifting Tools is deployed worldwide on behalf of developers and tier-one contractors The company recently worked with Venterra Group subsidiary offshore and geotechnical engineering company Gavin & Doherty Geosolutions (GDG) to deliver monopile driveability guidance for a vibro pile installation research project initiated by the Carbon Trust Offshore Wind Accelerator (OWA) “We are excited to foster this already close working relationship as they become part of the Venterra Group,” GDG said in a statement Venterra chief executive officer Rob Jewkes said: "Laurens and his team pioneered the use of the proven vibro hammer technology from onshore applications to offshore wind They have built a very successful business with a highly differentiated offering as the demand for ever larger turbine foundations accelerates." de Neef added: “We are proud of the innovation that has driven our growth in offshore wind Joining Venterra will enable continued investment in foundation installation in deeper waters and the ability to support larger turbines up to 18-20 MW “We share Venterra’s vision to create a sustainable supply chain and help the industry innovate at the pace needed to meet the needs of developers and our tier one clients Venterra’s footprint in the United States and APAC as well as Europe fits our own expansion plans as we deploy expertise gained from European wind farms across the global market.” Cape Investment Partners (CIP) bought out the majority shareholding in Cape Holland as well as offshore grouting business Found Ocean Cape Holland has been on an impressive expansion trajectory growing revenues manyfold and building a strong portfolio of projects and technologies in the renewable energy space CIP is proud to have been able to support this process during recent years." 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Subscribe to GE’s enewsletters and follow us on Twitter and LinkedIn Tagged with: Keller Group has reported a record revenue for the 2024 financial year on the back of strong market conditions in North America Transmission Investment has alerted the market to an upcoming £700M package of works on its project to build a subsea electricity interconnector between Northern Ireland and Scotland Card Geotechnics (CGL) has announced that South West Geotechnical (SWG) has become one of its divisions Keltbray Infrastructure Services has rebranded to Aureos after it was acquired by private equity fund EMK Capital earlier this year Sign in or Register a new account to join the discussion Thanks for visiting Sign In Register Thanks for visiting Canadian swim coach Mike Thompson thinks Alia Atkinson's best is yet to be seen Thompson says his interest in the Jamaican swimmer was piqued after she beat Canada's Tara Van Beilen in a swim-off at the London Olympics Her performances at the Canada Cup meet in November only served to strengthen his beliefs                            Atkinson turned 24 on the eve of the world short-course championship in turkey where she won two silver-medals Thompson says the modern day swimmer is progressively older so Atkinson's age should not pose a problem Copyright © 2025 Multimedia Jamaica Ltd Senior Staff ReporterSwimmer lowers national mark to make today's final IT WAS one of the longest one hour and 40 minutes of her life Jamaican swimmer Alia Atkinson kept her medal hopes alive and is the first Jamaican in a final at the Olympic Games in London Keeping herself hydrated and hopping to and from the restroom she waited agonisingly for yesterday's evening session as she prepared to face her destiny A spot in the final was on the line as the three-time Jamaican Olympian and Canadian Tera Van Beilen stood alone in the almost emptied Aquatics Centre; only one would leave the pool with a place among the top-eight 100m breaststroke swimmers on the planet The swim-off became necessary after the pair had tied for fourth place and the final qualifying spot in their semi-final earlier yesterday evening with a time of 1:07.48 which was slower than the 1:07.39 national record that Atkinson posted earlier That time made her the second Jamaican to win a swimming heat at an Olympic Games after Sion Brinn won his 50m and 100m freestyle heats at the Atlanta 1996 games Atkinson passed the time by keeping herself hydrated and getting pep talks from her mother and coach determined to become the first Jamaican to medal at an Olympic Games was not about to let her dream be taken away by the 19-year-old Canadian as she got a strong start and powered through after the 50m turn to stamp her class and secure her final berth "My main goal coming here is to make the final and I just told myself that she (Van Beilen) was not going to stop me," Atkinson asserted shortly after her race and is now stocked up on confidence heading into today's final "My mother told me that in order to make each step and I didn't do it (get better) for the second one (semi-final) so I had to do the swim-off; it was a lesson and it only means that I am improving each time," Atkinson told The Gleaner "My coach spoke to me and reminded me that I had more endurance than her because I do the 200m breaststroke as well so another swim is nothing compared to what I had done so far and I just needed to know for myself as well," she added Atkinson is also scheduled to compete in the 200m breaststroke and the 50m freestyle at these Olympic Games - her third after she competed at the 2004 Athens games and 2008 Beijing games andre.lowe@gleanerjm.com View the discussion thread. 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