Please enable JS and disable any ad blocker Thanks for visiting The use of software that blocks ads hinders our ability to serve you the content you came here to enjoy We ask that you consider turning off your ad blocker so we can deliver you the best experience possible while you are here we have begun the renovation of four large out of play areas (approximately 6.5 acres in all) to native prairie with the goal of supporting a diverse community of plants preparation and regular maintenance along the way Establishment of a what most would recognize as a prairie community can take at least three years but the establishment of a self-sustaining prairie community takes much longer Kampen Prairie is in the beginning stages of establishment and we are using this process to develop a clear picture of likely outcomes to help guide the efforts of other golf courses interested in converting out-of-play areas to naturalized areas dominated by native prairie vegetation There is increasing interest in converting golf course out-of-play areas to naturalized areas dominated by native prairie vegetation The rational for doing so includes a combination of economic golf courses undertaking such a project require science-based information that provides a clear picture of likely outcomes to help guide their efforts With the aim of supporting the golf industry in their efforts to enhance sustainability the long-term goal of the Kampen Prairie project is to understand the economic and ecological dimensions of renovating out-of-play areas to conservation habitat the first phase of this project is to evaluate the effects of seeding time (fall vs spring) and method (broadcast seed vs seed drill) on establishment success (floral diversity) and the ecosystem services (pollinator diversity) provided by each approach The conversion of out-of-play areas to native vegetation has the potential to 1) reduce maintenance costs associated with mowing 2) increase aesthetic appeal as a result of the color and richness of the prairie community and 3) enhance ecosystem services provided by the diverse assortment of pollinators and other wildlife that depend upon native vegetation The project will provide the golf industry with the first field scale comparison of different seeding approaches provide a clear picture of expected outcomes and quantify one aspect of the ecosystem services provided by these eforts Trouble with this page? Disability-related accessibility issue? Please contact us at ag-web-team@purdue.edu so we can help. Your Ads Privacy ChoicesIMDb Rylance confirmed the death of his wife on Saturday Claire van Kampen, a composer and playwright who was married to Wolf Hall actor Mark Rylance Van Kampen died on Saturday, January 18, which was Rylance’s 65th birthday, People reports surrounded by her family,” her family said in a statement “Claire has died of cancer on Mark Rylance’s 65th birthday and countless beloved friends in England and America.” Van Kampen was “one of the finniest and [most] inspiring women we have ever known,” her family added “We thank her for imbuing our lives with her magic After studying music theory and piano at the Royal College of Music in London, van Kampen joined the Royal Shakespeare Company in 1986, BBC News reports She composed the music for a 1989 production of Hamlet “Claire completely changed my life,” Rylance said in a 2023 interview with The Guardian “She introduced me to that world of classical and modern music and it was very much around music that we fell in love we were always imagining stories that we could tell together I’ve lost count of how many projects we’ve imagined Claire came to me with two children whom I raised with her and [her first husband] Van Kampen also composed scores for the Broadway productions of True West plus adaptations of Shakespeare’s Twelfth Night and Richard III She worked as an associate and senior research fellow for early modern music at Shakespeare’s Globe Theatre and a creative associate at the Old Vic Theatre the 2017 Broadway production of which starred Rylance She also notched a collaboration with her husband when she worked as Wolf Hall’s Tudor music advisor and arranger With her previous husband, architect Christopher van Kampen, Claire welcomed two daughters, actor Juliet Rylance and filmmaker Nataasha van Kampen the latter of whom died of a brain hemorrhage in 2012 Sign Up Van Kampen died on the 65th birthday of her husband David Gordon and director Claire van Kampen has died at the age of 71 The cause of van Kampen’s death was cancer; she passed on the 65th birthday of husband and longtime artistic collaborator Van Kampen was the founding director of theater music for Shakespeare’s Globe in London and composed the scores for the Broadway productions of Boeing-Boeing Van Kampen was Tony nominated for her play Farinelli and the King in 2018 and made her directorial debut with Rylance’s comedy Nice Fish She also directed Rylance and André Holland in Othello The composer for more than 30 productions at Shakespeare’s Globe van Kampen served as the arranger of Tudor music for the BBC series Wolf Hall and shared the 2007 Sam Wanamaker Award with Rylance and designer Jenny Tiramani Wife of architect Christopher van Kampen and she was mother of two girls with Christopher Nataasha died in 2012; she is survived by Mark and Juliet Rylance The musical opens at the Imperial Theatre on April 10 Get the best deals and latest updates on theater and shows by signing up for TheaterMania's newsletter today playwright and theatre director who often worked with her husband Claire van Kampen, who has died of cancer aged 71, was a concert pianist, a musical director and composer of some distinction. Latterly she was a playwright and director, often in association with her husband, Mark Rylance Their most distinguished collaboration was on the West End and Broadway hit Farinelli and the King (2015) a candlelit piece (written by Van Kampen) based on the true story of King Philip V of Spain (played by Rylance) who took refuge from his bipolar depression in the ethereal singing of the famous Italian castrato Farinelli (sung by the countertenor Iestyn Davies) Sometimes compared by critics to a cross between Peter Shaffer’s Amadeus and Alan Bennett’s The Madness of King George III the piece was more fragile and suggestive than either cleverly juxtaposing a quasi-bipolar condition in Farinelli himself shadowed by the singer achieving celestial sublimity not least in two great arias from Handel’s Rinaldo Farinelli and the King at the Duke of York’s theatre The play about the famous castrato Farinelli was written by Claire van Kampen Photograph: Tristram Kenton/The GuardianVan Kampen incorporated other staples of the Renaissance castrato repertoire in Farinelli and the King Van Kampen having become much taken with it after meeting one of the founders of the Early Music Consort Her research into the music of the Tudor period placed her in an ideal position to complement Debbie Wiseman’s score with Tudor musical interludes for the blockbuster TV production of Hilary Mantel’s Wolf Hall (2015); she also complemented the performance of Rylance as Thomas Cromwell The couple had met in the rehearsal room at the National Theatre in 1987 a baggy 19th-century prose work by Eugène Sue a life-changing moment that took him towards her kind of classical music Van Kampen was first married to the architect Chris Perret but he agreed to a divorce – they had two daughters Van Kampen was musical director at the Royal Shakespeare Company and wrote the music for Rylance’s temperamental and produced a 1991 new age version of The Tempest (Rylance as Prospero music byVan Kampen) in a muddy Oxfordshire field they had been married on the winter solstice in December 1989 The same production played at the Globe (now Gielgud) on Shaftesbury Avenue but not before it had already visited Corfe Castle and the cement pit on the South Bank of the Thames that formed the foundation of the future Shakespeare’s Globe in which Rylance stole the show as a wheedling Iago Claire was the only child of Paul van Kampen so her mother went out to work as a secretary to pay for Claire’s piano lessons though she was already teaching pupils of her own by the age of 14 She trained at the Royal College of Music for five years and graduated as a concert pianist and composer with Rylance as a Stan Laurel-esque stooge to Roger Allam’s lascivious adventurer In 2013, the New York Times congratulated her on bringing sackbuts to Broadway for the Globe’s all-male productions of Twelfth Night (Rylance as Olivia) and Richard III (guess who) She shared the composer credits with the Elizabethan maestros John Dowland and Thomas Morley Another wacky project with Rylance was her production of Nice Fish (2016) written by Rylance with the poet Louis Jenkins which offered complimentary tickets to anyone who turned up at the Harold Pinter theatre dressed as a fisherman Her last theatre work, in 2024, was an evocative score for the RSC’s Pericles in Stratford-upon-Avon and another for Juno and the Paycock by Sean O’Casey at the Gielgud In 2019 Van Kampen was honoured with a doctorate in music from Brunel University She is survived by Rylance and her daughter Juliet born 3 November 1953; died 18 January 2025 Claire van Kampen, a musical director and Tony-nominated playwright who frequently worked with her husband Mark Rylance said on social media that the cause was cancer Having studied music theory and piano at the Royal College of Music in London she became the musical director of the Royal Shakespeare Company in 1986 She met Rylance working on a production of The Wandering Jew at the Royal National Theatre in 1987 she was appointed Director of Theatre Music for the newly reopened Shakespeare's Globe Theatre in 1997 creating music for 30 productions during Rylance’s term as artistic director and beyond Van Kampen went on to compose original music for the Broadway productions True West (2000) Boeing-Boeing (2008) and La Bête (2010) arranger and musical director for Twelfth Night and Richard III which starred Rylance and featured a pit band of musicians playing Elizabethan instruments about the 18th-century Italian castrato singer and King Philip V of Spain Tudor-era music selected and arranged by Van Kampen featured on the soundtrack for television’s Wolf Hall Farinelli and the King transferred to Broadway the following year earning five Tony nominations including Best Play Van Kampen is survived by her husband and a daughter from her first marriage Van Kampen was "one of the funniest and (most) inspiring women we have ever known," the family said in a statement "We thank her for imbuing our lives with her magic One of the great wise ones has passed." director and playwright of Farinelli and the King is remembered by the Globe’s former artistic director Claire van Kampen, theatre director and composer, dies aged 71 Claire van Kampen, theatre director and composer, dies aged 71 On the first afternoon of rehearsals for any new show I would ask Claire to push past the awkwardness and nerves of new beginnings with some group singing her galvanic energy and shining eyes would have a group of strangers harmonising together with a gusto they had not felt since childhood when she first went under palliative care in a German clinic a devoted community on WhatsApp formed and then grew Anecdotes of Claire in excelsis were shared some tender and others filthy; old photos of her dazzly smile accumulated; insights which Claire had rained on the world for free were appreciated; and tears were spilt over how remorselessly she had spread light Claire could still get a wide and diverse group from across the world to sing together she became one of the world’s great theatre composers No one has ever set a Shakespearean song better than her – her versions of the closing songs to Twelfth Night and Love’s Labour’s Lost are definitive; no one knew better how to drive a story with percussion; how to detonate some humour into a room with some oompah nor how to make 1,000 hearts skip a beat with a single plaintive human voice The Renaissance and her were natural companions – something about the openness and candour of that age A first time writer with a Tony nomination All born out of a woman’s ferocious and proud resolution that tragedy would not define her That miracle was in large part down to Mark but yet more her comprehensive theatrical nous A sort of preternatural joy emanated from her She told Mark recently it had always been her desire to live life as “a splash of light for others” Unkind souls had said she was only at the Globe in its first 10 years because of Mark: I often thought we were only there because of her Life with Claire was always outrageous fun Somehow she could sustain the look of someone who had just woken up whether in a dingy shebeen or a potentate’s palace she had an old-fashioned dervish desire for whatever joy the world might offer They saw the world in good-old polytheistic Technicolor and wondered why others succumbed to black and white What I most treasured about her was her intelligence by which I don’t mean the thin soup of university learning but the rich feast of reading the world and art and people with brilliance The best word to capture that capacity is that old Germanic bright of heart when colleagues or friends needed light The fuel for all that radiance came from an intimate circle; her first husband Chris still with her at the end; her daughter Nataasha who though she predeceased her never left her; Juliet who somehow graduated from daughter into a trinity of daughter with whom she formed a union wild and antic A great many of us were blessed to live nearby A fire at a home on Kievitstraat in Kampen early on Monday morning left the two residents injured and caused considerable damage to the home. The blaze was started by an overheated tablet charger in the attack, residents told RTV Oost The fire brigade responded in large numbers because the flames were developing quickly and the residents were still inside The residents told the broadcaster that an overheated charger caused the fire It was charging a tablet in a bedroom in the attic when it set fire to a pouffe One of the children in the house tried to take the pouffe outside The boy suffered burns to his hands and smoke inhalation He was taken to the hospital for treatment His mother also went to the hospital to be treated for smoke inhalation This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page She graduated from DeForest High School in 1952 and married Wayne Ihland on May 16 1953 at Zion Evangelical Lutheran Church in South Leeds Audrey worked at Ohio Chemical for three years then two years at IBM in the Service Bureau In 1958 she started working at Oscar Mayer in the computer room and retired in 1992 after working there 34 years Audrey enjoyed traveling with Wayne and their many friends the Holy Lands and the many bus trips throughout the United States She also coordinated a ladies’ bus trip to the annual Gem and Jewelry Show in Chicago for 22 years especially her many euchre and sheepshead games and their beautifully maintained yard was evidence of it who were killed in a car accident on November 21 Ove and Stella Ihland; and brothers-in-law A memorial service will be held at 11:00 a.m with Pastor Schwertfeger and Pastor Scott officiating until the time of service on Monday at the church Burial will follow at Zion Lutheran Cemetery with lunch following at the church Memorials may be made to the Columbia County Health Care Center To view and sign this guestbook, please visit: www.ryanfuneralservice.com Ryan Funeral Home & Cremation Services To view and sign this guestbook, please visit: www.ryanfuneralservice.com had a decision to make his senior year of what athletic sport he would want to continue at the next level More: Jonesville '24 grad Gracen Hager will make an impact for Jackson College Jets volleyball Van Kampen was a state qualifier for the boys cross country team He was an all-state runner for Jonesville track his final senior year helping lead the team to runnerup relay honors at this year's state finals he was a dream team basketball honoree for the Comets helping lead the team to their 2022-23 district and conference championship More: Jonesville graduate Damon Newell brings home-run hitting to St. Scholastica baseball Van Kampen made the decision to play for the Kalamazoo College men's basketball team He was also offered a spot on the track and cross-country teams but I think basketball is more my passion and I’ve grown into basketball more," Van Kampen said a NCAA DIII member of the Michigan Intercollegiate Athletic Association were one of the options Van Kampen was debating on continuing his athletic and academic career at His desire to continue basketball at the next level was largely based on his 12 years playing for Jonesville athletics programs I should just keep playing as long as I can They have really good athletics and really good academic programs Van Kampen credited his cross-country coaches Josh Perrin and Jake Snyder basketball coaches Jeff Turner and Jason Elder and his teammates for helping him make that decision to pursue collegiate athletics “Coach Turner really helped me put my name out there for helping me put myself out there and showing me what I got to do.”  is another signee from this class of Comet basketball seniors More: Camden-Frontier's Ethan Wickham set to be an impact receiver for Bluffton University Van Kampen plans to pursue a degree in business at Kalamazoo College but is not yet sure what career path that will take him in after college graduation A three-year varsity player for the Comets Van Kampen was on three winning basketball teams under coach Jeff Turner The 2021-22 season saw the team win 13 games winning the Big 8 conference championship and winning a district title They made a second-straight district title game appearance after beating county rivals Hillsdale (54-52) and former league rivals Concord (54-51) They lost to champion Hanover-Horton 66-49 in the title game More: Seven seniors set the bar for future Comet boys basketball success in the Cascades league He finished his senior year averaging 11.6 points-per-game He had an overall field goal percentage of 55.3 and a two-point percentage of 54.6 He was an all-region athlete three of his cross country seasons He earned runnerup honors at the conference championship took fourth at regional and took 40th at the state final held at Michigan International Speedway He earned 2023 all-area dream team honors for his efforts More: Brooke Smith and Thomas Holm named Cross Country Runners of the Year; 2023 Dream Team Van Kampen had an impressive career for Jonesville boys track and field he was a state qualifier on the 4x800 relay team He was a state qualifier in the same event his junior year and also in the 4x400 He helped lead the 4x400 team that included David Fowler Damion Scharer and Brady Wright to a sixth-place all-state finish His senior season saw him earn three state qualifications Van Kampen and Caleb Blonde took fifth and all-state honors the conference championship and the regional championship More: Jonesville track earns program best finish at D3 state finals; 4x400 team earns runner-up He helped the 4x400 team set the new school record time More: A school record fell as Jonesville track athletes win at regionals and qualify for state Parchment's Jalen Kampen reacts after sealing a victory during a boys basketball game between Parchment and Kalamazoo Hackett at Kalamazoo Hackett High School in Kalamazoo .st1{fill-rule:evenodd;clip-rule:evenodd;fill:#2a2a2a}By Matthew Ehler | mehler@mlive.comPARCHMENT MI –– One of Parchment’s all-time greats has found a new basketball home flipped his commitment from Ferris State to Central Michigan on Friday The all-state guard’s decision comes after longtime Ferris State coach Andy Bronkema was hired as the Chippewas’ new head coach last Tuesday #AGTG 100% COMMITTED!! 💛💛@CoachBronks @PPanther_Hoops @CMUMensBBall pic.twitter.com/VmDHMrFFid built Ferris State into a perennial contender in Division-II highlighted by winning a national championship in 2018 the McBain native inherits a CMU roster with just one player But other Bulldogs could follow Bronkema soon The 6-foot-4 prolific scoring senior enjoyed a highly decorative Parchment career and flexing over 70 wins in four varsity seasons boasting a career-best 22.2 points per game this winter He also shot 87 percent from the free throw line and 36 percent from 3-point land and dropped a career-high 40 points in a win over Three Rivers this season That production earned Kampen MLive/Kalamazoo Gazette’s Dream Team Player of the Year honors “He’s in rarified air,” Parchment head coach David Jones said in a previous MLive story “That (scoring) record will last quite a while But it’s because he’s put in a lot of work to get to this level and he’s been blessed with physical skills When you combine those two things together you get a chance to be something special.” Kampen finished as an 80.1 percent free throw shooter which ranks 17th all-time in MHSAA history Kampen walked away from his prep hoops career a four-time SAC-Valley honoree a four-year starter and a two-time Associated Press Division 2 all-state selection Use of and/or registration on any portion of this site constitutes acceptance of our User Agreement, (updated 8/1/2024) and acknowledgement of our Privacy Policy, and Your Privacy Choices and Rights (updated 1/1/2025) © 2025 Advance Local Media LLC. All rights reserved (About Us) The material on this site may not be reproduced except with the prior written permission of Advance Local Community Rules apply to all content you upload or otherwise submit to this site YouTube's privacy policy is available here and YouTube's terms of service is available here Ad Choices #AGTG 100% COMMITTED!! 💛💛@CoachBronks @PPanther_Hoops @CMUMensBBall pic.twitter.com/VmDHMrFFid Your Ads Privacy ChoicesIMDb, an Amazon company© 1990-2025 by IMDb.com, Inc. Kimberly van Kampen, whose Hampton Green Farm and Discover Dressage are major sponsors at World Equestrian Center, firmly supports expanded dressage schedule at Ocala as a response to a major investment in new facilities and cites uncertainty over the future of the competition schedule of Wellington’s Global Dressage Festival. Kim who moved from Wellington where she was a partner in the equestrian group that owned the main hunter-jumper show that staged the decades-long Winter Equestrian Festival and sponsored Global’s covered arena, formally opens her new Hampton Green Farm in Ocala next month which will also be the breeding and development center for P.R.E. horses that had been in Michigan. Of the remaining 12 in the U.S., seven are in Southern California and the other five spread across the U.S. throughout the year. With the expanded schedule, Florida has four more dressage events for comparable levels than in all of Germany over the same period, according to the FEI schedule. Growth of the Ocala circuit comes at the same time as new organizational management headed up by Noreen O’Sullivan, an organizer who had operated multiple dressage events in and around Wellington before Global was established in 2011. And this week, the regional championships of national levels for Alabama, Florida, Georgia, South Carolina and Tennessee lists entries of more than 540 horses with more than 430 riders over five days at Ocala’s WEC–easily able to handle the horses with almost 3,000 climate-controlled stalls and two multi-story hotels for grooms, riders, owners and spectators on the show grounds that cover 300 acres/121Ha. “But the other thing is, I just don’t know what’s going to happen in Wellington and when you have a vacuum you’ve got to fill it with with something or somebody else is going to fill it.” Approval has been given by the government of Wellington for the Global grounds to become a high end residential and golf community in exchange for creation of a dressage area alongside the main hunter-jumper grounds. However, the sale of the main show grounds as well as the area theoretically designated for dressage plus a jumping course has not yet been completed. If Global moves to an area adjoining the main hunter-jumper arenas, in Wellington, she speculated, dressage will share the competition space with hunters and jumpers. “If dressage doesn’t have its own showgrounds, it will not be a high priority in Wellington. And space is so limited and it’s so expensive down there. And to start a brand new showgrounds and build it from the ground up is going to be a very, very expensive project. And I don’t know if that’s going to happen.” Hunter-jumpers are a much bigger community than dressage, and the main show grounds are at capacity. Speculation is that when sale of the main show grounds occurs and expanded to include the area for dressage, Global may be reduced from its current schedule of 12 weeks that includes national and para dressage competitions in addition to international events could be reduced to provide more competition arenas for the much larger hunter-jumper circuit. While details of the future lineup of dressage events is uncertain, growth at Ocala’s WEC and TerraNova near Sarasota has already created what is being dubbed a dressage “super circuit.” Van Kampen was also a writer, playwright and accomplished musicologist Claire van Kampen, a playwright and director who composed music for TV and theater and the wife of Mark Rylance, has died, her family said in a statement Saturday. She was 71. Van Kampen, also a concert pianist and theater director, died in Germany surrounded by family. No cause was given. Her music was featured on Broadway and in London productions including “Richard III,” “Twelfth Night” and others with her husband in the cast. Van Kampen worked at Shakespeare’s Globe for nearly 20 years, eventually becoming its first female director. She also wrote “Farinelli and the King,” which Steven Spielberg urged her to turn into a screenplay, and composed the score for the production that started in London before it moved to Broadway. Van Kampen and Rylance were working on a historic TV drama for Spielberg’s Amblin Entertainment at the time of her death. The family statement provided to TheWrap is below: Our beloved mother, and wife, Claire Louise van Kampen, has passed peacefully this morning, Saturday the 18th of January at 11:47, in the ancient town of Kassel Germany, surrounded by her family. Concert Pianist, Composer, Musical Director, Theatre Director, Playwright, Mentor and Friend.  And one of the funniest, wickedest, most inspiring women we have ever known. Claire has died of cancer on Mark’s 65th Birthday.  We thank her for imbuing our lives with her magic, music, laughter, and love. Ring the bell, sound the trumpets reverie, something is done, something is beginning. One of the great wise ones has passed. Several guests at the Pony Bar in Kampen allegedly sang "Germany to the Germans — foreigners out!" in the Pony Bar in Kampen last May, which led to an investigation on suspicion of incitement to hatred only one 26-year-old man was "publicly charged" for a "waving greeting" with an outstretched arm and the suggestion of a "Hitler moustache," according to the press release. These gestures were clearly seen in a video that went viral on social media he was ordered to pay €2,500 (around $2,800) to a charitable organization as a condition of probation Further proceedings for incitement to hatred were dropped. If he accepts the fine the case against two men and a woman was dropped because shouting the slogans shouted in the video did not constitute the crime of incitement to hatred which was captured in a video lasting just a few seconds and uploaded to social media without comment It showed several young people chanting "foreigners out" and "Germany for the Germans" on the terrace of the pub during a party to the hit song "L'Amour Toujours" In the weeks that followed, other incidents involving the song were reported including public incidents in Bavaria and Mecklenburg-Western Pomerania Wife of actor Mark Rylance worked in various roles at Shakespeare’s Globe and had been diagnosed with cancer Claire van Kampen: a world-class theatre composer and bright star of Shakespeare’s Globe dies aged 71This article is more than 3 months oldWife of actor Mark Rylance worked in various roles at Shakespeare’s Globe and had been diagnosed with cancer Claire van Kampen: a world-class theatre composer and bright star of Shakespeare’s Globe The director and composer Claire van Kampen, the wife of the actor Mark Rylance has died at the age of 71 after being diagnosed with cancer She died in the central German town of Kassel surrounded by her family on Saturday morning a statement shared on behalf of Rylance and her daughter Juliet said Saturday also marked Rylance’s 65th birthday She worked in various roles at Shakespeare’s Globe for around 20 years Her family described her as “one of the funniest and [most] inspiring women we have ever known” They added: “We thank her for imbuing our lives with her magic Van Kampen trained at the Royal College of Music in London where she studied music theory and piano specialising in the performance of 20th century music She went on to have a career as a composer and performer television and film soundtracks and the concert hall In 1986, Van Kampen began her theatre career with the Royal Shakespeare Company and with the Royal National Theatre the following year She served as artistic associate at Shakespeare’s Globe from 1996 to 2006 Van Kampen later acted as a musical consultant and resident composer to the subsequent artistic director She was also a creative associate of the Old Vic theatre in London Van Kampen’s theatre credits include composing the music for the 1989 Royal Shakespeare Company production of Hamlet starring Rylance Later in her career she created original scores for Broadway productions of 2000’s True West 2008’s Boeing-Boeing and 2010 comedy La Bete as well as adaptations of Shakespeare’s Twelfth Night and Richard III Van Kampen also wrote the play Farinelli and the King, which saw Rylance star as King Philippe V of Spain and was nominated for several Olivier awards including best new play Rylance was asked about his creative relationship with his wife in an article for the Guardian in 2023 He said: “Claire completely changed my life We met at the National Theatre when she was musical director of a play I was in “We’ve always loved working together, from Phoebus Cart, our own theatre company in the 90s, to our years at the Globe, to Farinelli and the King Van Kampen was previously married to architect Christopher van Kampen with whom she had two daughters: Juliet and Nataasha died aged 28 in 2012 after suffering a brain haemorrhage The Glenn Korff School of Music’s Jazz Singers will perform at 7:30 p.m. March 12 in Westbrook Recital Hall Room 119. The concert is free and open to the public. It will also be webcast live. Visit the concert's website on the day of the performance for the link The Jazz Singers are under the direction of Glenn Korff School of Music Lecturer David von Kampen.  The Jazz Singers will be joined by special guests Elkhorn North High School’s “ENsync,” under the direction of Casey Allen and the Nebraska Vocal Jazz Project.  The program begins with the Nebraska Vocal Jazz Project performing four arrangements by von Kampen including Sammy Fain and Lew Brown’s “That Old Feeling,” Sarah Jarosz’s “Orange and Blue,” Gabriel Kahane’s “Little Love” and Dave Frishberg’s “Little Did I Dream.” “ENsync” will perform Sammy Fain and Irving Kahal’s “I’ll Be Seeing You,” arranged by Phil Mattson and Paul Simon’s “Loves Me Like a Rock,” arranged by Darmon Meader They will then be joined by the UNL Jazz Singers to perform Sarah Jarosz’s “Green Lights,” arranged by von Kampen the UNL Jazz Singers will close the program with Ray Davies’ “You Really Got Me;” “Elsa,” with music by Earl Zindars; Radiohead’s “Exit Music (For a Film);” and von Kampen’s “Color Theory II,” all arranged by David von Kampen “This will be such a special night with three groups that are remarkably interconnected,” von Kampen said “Nebraska Vocal Jazz Project is comprised entirely of former members of UNL Jazz Singers is the vocal music director at Elkhorn North Former Jazz Singers soprano Annie von Kampen is currently student teaching at ENHS with Casey and current soprano Alyssa Schade has also been working with the ENHS choirs this year.” The Jazz Singers are also connected to Elkhorn through Jacob Samek the Elkhorn North High School graduate and Jazz Singers tenor “It’s been a difficult time for both groups but we are so glad for the opportunity to collaborate on this concert which will include the groups joining together on an arrangement of Sarah Jarosz’s beautiful song Some parts of this site work best with JavaScript enabled Wärtsilä Investor Relations organised a site visit to the Central Distribution Centre Kampen (CDC) in the Netherlands on March 27 provided a comprehensive overview of Wärtsilä's future growth prospects in the Services business and allowed the guests to witness firsthand the operational efficiency and sustainability in logistics.  The presentation material from the event can be found here Services is a key driver for Wärtsilä growth and profitability The event commenced with a presentation by Stefan Wiik who showcased the impressive growth of our services business over the past years emphasising its significance for Wärtsilä’s overall growth and profitability services grew by 9% compared to the previous year The main drivers of this growth include an expanding installed base The Parts and Field Service team in Wärtsilä supports all our service delivery models Wärtsilä’s installed base is served through a world leading global network of service centers and service professionals in 70+ countries Marine and Energy have approximately 3,400 billable professionals around the globe serving the company’s ~ 9,000 active customers Operational efficiency and sustainability as the main factor behind the Wärtsilä Global Logistics Services’ success Wärtsilä Global Logistics Services (WGLS) plays a fundamental role in Wärtsilä’s service strategy by providing spare parts logistics to Marine and Energy customers worldwide Head of Global Parts Management and Supply Management presented insights and key figures to Wärtsilä’s spare part business before the visitors could have a tour around the impressive logistic centre that covers a surface of 37,000 m2 Packing approximately 3,800 delivery lines every day something that was clearly visible to the visitors in the warehouse These volumes are possible thanks to CDC automation which in 2024 handed 97% of all pickings at this site Automation is not only crucial for maximizing efficiency and speed in the process but also bringing reliability Wärtsilä's continuous improvement mindset is evident also in WGLS On-time delivery has improved by 18% since 2021 To the question of what it would take to get the on-time delivery to 100% Gianluca commented that even though planning is crucial the delays are mostly due to external factors such as disruptions in shipping routes or challenges in customs Wärtsilä Global Logistics Services delivers high-quality services through a seamlessly integrated supply chain that is very focused on sustainability WGLS in Kampen uses eco-friendly packaging and has achieved a 67% reduction in plastic use since 2022 equivalent to approximately 35 tons per year all domestic truck transport in Kampen uses HVO100 fuel and we work closely with our partners and suppliers on it Today our sustainability impact goes beyond Wärtsilä and our direct customers and suppliers as our suppliers are proposing the same actions to their other customers It’s a domino effect and something we are extremely proud of," said Gianluca The day concluded with an extensive tour to the warehouse led by Kim Björknäs The visitors could see the operations from inbound to the delivery of the parts and everything in between and get proof point to what they had heard during the presentations.   The investor site visit to Kampen provided a clear picture of Wärtsilä's strategic direction and operational excellence Wärtsilä is well-positioned to continue delivering value to its customers and stakeholders The visit underscored Wärtsilä's commitment to innovation and excellence in the Marine and Energy sectors setting a solid foundation for future success Marine Service has grown quite rapidly over the years What kind of a growth rate can we expect going forward?  On March 31 Wärtsilä announced new financial targets including combined target of 5% annual organic growth for Marine and Energy we haven’t disclosed any separate target Services is one of the key drivers for the company’s net sales growth Growing installed base is supporting our service growth Moving up the service value ladder has the potential to increase sales EUR/kW by 2–3x relative to transactional You mentioned that you can make 2-3 times more business with agreement customers in Marine What kind of business you do more with them By using real-time data and advanced analytics to monitor the equipment we predict potential issues before they occur and therefore minimize downtime and reduce operational costs What are the most common spare parts you sell as well as turbocharger spares and automation spare parts How does Wärtsilä’s service offering differ from its competitors?  Wärtsilä stand out from competitors due to our good spare part availability widest in-house service network and our comprehensive range of solutions How much of your R&D investments are going to service?  Wärtsilä invests ~4% of net sales in R&D to accelerate the development of decarbonisation-related technologies There are strong R&D synergies between marine and energy but also between new equipment and service as we only service equipment manufactured by ourselves.  We have not disclosed the level of R&D investment in service it is reasonably hard to calculate due to the close development and synergies between equipment and service 1/51Kalamazoo Hackett hosts Parchment in boys basketball.st1{fill-rule:evenodd;clip-rule:evenodd;fill:#2a2a2a}By Matthew Ehler | mehler@mlive.comKALAMAZOO MI –– With a defender up in his grill and no air space in crunch time all of Jalen Kampen’s avenues to shake free seemed to point toward a dead-end path Kampen found a loophole and snuck behind Kalamazoo Hackett’s defense for a clutch back door bucket in the final seconds to send red-hot Parchment to a thrilling 54-51 victory on Friday –– the Panthers’ seventh consecutive win 5-0) reinforces its command atop the SAC-Valley conference with Friday’s win the only team in the conference with an unbeaten record “They were face guarding me the entire game so I knew I could just cut back door,” Kampen said of his go-ahead score “I saw my defender cheating above the screens that was going to be set for me and I just faked that way and cut back door FINAL: @Ptown_hoops 54, @HCPBoysHoops 51. @jalenkampen2025 comes up clutch with this bucket underneath in final seconds and the Panthers hang in a thriller — their 7th win in a row. What a game! Kampen led all scorers with 22pts. pic.twitter.com/g3l08e92BN Kampen stood on that business by pouring in a game-high 22 points including a stellar 12-of-13 night from the free throw line while junior Don’Jae Gilmore-Slater chimed in with 14 points –– eight of which came in the fourth quarter Parchment built a 12-6 lead after a quarter and led 26-23 at the break as Hackett used a 16-point third quarter to take its first led With his team trailing by one with under 15 seconds remaining the Ferris State commit broke free from the Hackett defense received a pass from Gilmore-Slater along the baseline and smoothly dropped in the game-winning layup underneath Hackett’s ensuing triple try from David Verduzco drew front iron and Kampen iced things with a pair of free throws in the final seconds –– sending the Panthers to an emphatic road win and I knew that once he had his man over to one side he was going to backdoor cut and get a wide-open layup,” Gilmore-Slater said “Coach (David Jones) always knows when to call the right plays to know when it’s going to work.” Parchment head coach David Jones opted not to call a timeout –– he believed his out-of-bounds play would serve up a clean look I thought we could score on that play,” Jones said “It took them a little while to get the ball in but give Don’Jae credit for making the pass and Jalen for finishing.” Parchment trailed 43-36 with just over five minutes remaining a spot that drew plenty of frustration and stagnation and yet that didn’t deter the team’s fourth quarter rally “I think we got down on ourselves a little bit there and it looked like we might roll over but gathered things back up and finally made a couple of shots,” Jones said “We just didn’t shoot it very well and give Hackett credit for their defense But we’ve talked about on the nights that we don’t shoot it well I’d say look this one up (in the dictionary) It all started courtesy of a pair of big-time 3-pointers from Gilmore-Slater while Kampen’s steal and coast-to-coast layup to pull within 49-48 with under two minutes to go “We were down and I feel like we needed a shot,” Gilmore-Slater said “I always trust my shot and I’ve got a lot of confidence in it Jalen gave me the ball and I knew I had to come up big for my team.” The Panthers pulled ahead courtesy of Caden Smith’s offensive rebound putback setting up a frantic final minute from both sides Hackett sophomore Elijah Brooks sunk a layup out of a timeout as the Irish retook the lead with 30 seconds remaining –– only for Parchment to dial up its decisive playmaking “We all get a little too wound up sometimes but we know that with the type of bond that we have “That was probably the worst game we played all year But Hackett certainly felt the win was theirs for the taking especially after a spirited second half sent them to a fourth quarter lead “We just had several empty possessions where we turned the ball over on various plays,” Hackett head coach Ryan Basler said “Our turnovers lead to transition points and that’s what happened The game was kind of lost in that moment and we let (Parchment) back into it they didn’t quit and made some tough shots.” the Irish have dropped two straight –– albeit to No It’s a stretch Basler believes will only sharpen them for tournament time “We’re trying to build a culture here where you put God first and others and then yourself,” he said “And until we have a complete buy in from everyone on our team then these we’ll continue to see these outcomes “I think we’re getting better but we’re still not one cohesive unit yet.” Brooks Basler led Hackett with a team-high 17 points Parchment will host Battle Creek Pennfield on Tuesday Parchment senior guard Jalen Kampen poses ahead of the 2024-25 season.(Courtesy photo / David Jones) MI –– One thousand four hundred and eighteen It’s a number that’s locked in Jalen Kampen’s head a feat that would ascend him atop Parchment’s all-time scoring list and something he’s set as an important objective entering his senior season and while breaking that record is (a major goal) who eclipsed 1,000 points for his career late last week “A lot of people said that we haven’t been good since my freshman year when we won districts RELATED: Kalamazoo boys hoops highlights: Ferris State commit reaches 1,000 points at Parchment After battling through an injury-riddled and bruising junior season one that saw the Panthers bow out sourly in a district opener to Plainwell the 6-foot-4 combo guard and Ferris State commit has major intentions on making his mark in Parchment history “We had some guys that didn’t really care as much but I feel like you can see the love of basketball with this team,” Kampen said “And that’s not even if they are a basketball player because the love we had for football was out of this world and it’s translated into every sport.” That all starts with a rare 11 seniors on this year’s roster with a majority of them powering Parchment football to a successful seven-win season in the fall who still found time to work on his game –– and he’s ready to unleash it this winter “I put a lot of work in over the offseason I feel like I’m a more efficient with the basketball now.” Kampen is some 400 points behind Parchment’s all-time scoring leader Trey Zachary who finished with 1,418 career points (1997-01) Zachary also holds the single-game school record for points (49) and single-season record for points (494) 6.5 rebounds and 3.1 assists –– all while shooting 40 percent from 3-point land and 52 percent from the field –– last season en route to first team all-state honors Kampen said he put a large emphasis on becoming a more well-rounded scorer this offseason “I’ve been working on finishing through contact getting better at my mid-range game and becoming a three-level scorer That’s already been on full display to start this season as Kampen dropped a season-high 32 points to eclipse 1,000 points for his Parchment career in a 61-8 victory over Constantine late last week along with being a four-year varsity starter Parchment head coach David Jones said Kampen has helped set the tone for the Panthers’ program he’s just a phenomenal athlete and he’s got a ton of experience” Jones said “He’s started nearly 70 varsity games and he has over 50 wins in those games so he’s definitely used to winning But that’s the expectation we’re trying to set and hopefully fans and alike will say ‘they’re a fun team to watch.’ That’s the biggest thing and winning on top of that makes it even better.” along with promising juniors Donjae Gilmore and BJ Hunt Jones believes his team has a “very good chance” at being one of the best Parchment teams he’s coached “I think we have a chance to be pretty good because we’re an athletic group that all seem to get along,” Jones said and I’ve never had anything like that as a coach We had a good chance to be a 20-win team last season but we had our two best post players go out with knee injuries and Jalen missed five games with an ankle injury But I think we’ve got a real good chance to be very competitive.” But who will be Parchment’s deciding X-factor to send them over the top 1/25Parchment hosts South Haven in high school football 2024.st1{fill-rule:evenodd;clip-rule:evenodd;fill:#2a2a2a}By Matthew Ehler | mehler@mlive.comPARCHMENT MI – Jalen Kampen is a basketball player at heart but he doesn’t enjoy going about his football business in half-hearted fashion Kampen ran rampant and was the football maestro that stamped a remarkable five-touchdown performance –– igniting Parchment to a dominant 32-0 win over South Haven on the Panthers’ homecoming Friday night “Basketball is obviously my main sport but if I play a sport I’m not just going to halfway it,” Kampen said “We were the underdogs tonight and we noticed that we were predicted to lose 41-20 This game was personal because we got whooped last year South Haven had little answer for the dual-sport and dual-threat senior quarterback as he often darted and danced around Rams and rifled passes to his teammates en route to a 353 all-purpose yard night “I told them this week that it’s about darn time that he and Jaden (Taylor) take over a game and show everyone why you’re the best players in the area,” Parchment head coach Richard Roth said Kampen was the orchestrator –– but the Panthers were all in sync on Friday “We’ve been talking about the fact that we have to put people on notice,” Roth said “Our motto this week was that we were the underdogs We have to go prove it to people that we are who we think we are.” as a trio of Kampen scampers setup his first strike of the night –– a corner route dot to senior Jaden Taylor for a 13-yard touchdown and a 6-0 lead flexing an 18-play and over eight-minute drive of their own that bled into the first play of the second quarter On fourth-and-goal from the Panther 1-yard line Parchment’s goal line stand jolted their offense as Taylor broke free for a 40-plus yard gain to give them some breathing room and Kampen did the rest The Ferris State commit fooled the entire South Haven defense on a fourth down option keeper jump cutting his way for a 10-yard touchdown and a 13-0 advantage at the break and I was able to make good reads,” said Kampen who finished with 124 rushing yards and three touchdowns along with 229 passing yards and two more scores “We’re not going to give up and we’re going to play to the end of the snap.” In his second year under Roth’s spread option air raid offense things are starting to click for the signal caller “He’s comfortable in his own skin,” Roth said “You need to go out there and be a calming influence at that position and he’s done that very well as of late and he best part of having him on the field is that when I make a dumb call Parchment forced a quick three-and-out and watched Kampen get back to work in the third quarter in engineering a nine-play drive capped off by his third score TOUCHDOWN: After Parchment’s defense forces 3&out, @jalenkampen2025 caps off this fine Parchment drive with a nifty 10-yd TD run - his 3rd score of the game. Panthers lead 20-0, 4:55 left 3Q. @ParchmentAD @JaredPurcellDET pic.twitter.com/2azfxP3MBD Parchment’s Isaac Gostlin would strip the ball from Blake Bosma and recover a fumble moments later and Landon Meredith stepped in front of a Cade Hahn pass early in the fourth quarter for an interception The two-way athlete secured a bubble screen and darted up field shedding a few tackles and hitting the jets for an electrifying 56-yard touchdown to firmly put the Panthers in the driver’s seat “I feel like I’ve been under the radar and I’m really trying to get my name out there,” said Taylor who finished with 82 yards rushing and 86 yards receiving everybody was making us the underdogs and we had something to prove Hopefully people start seeing us as the real deal.” That was perhaps hard for South Haven to see especially after losing to Constantine by just six points last week we just didn’t execute them,” South Haven coach Mark Eddy said “We just had too many unforced errors and if you can’t take care of your own business Parchment was pretty sharp and we knew they had some senior players that would make plays on us but they just executed at a higher level than we did.” Eddy said his group wasn’t locked in on their opponent –– something they hope to shore up “We just didn’t have the right focus,” Eddy said “If you think the team isn’t high enough of caliber then you don’t put yourself in the right mindset Hopefully we’ll think differently and make changes so that we can continue to improve.” Kampen ensured no doubt with his third and final rushing score in the waning moments –– an exclamation point to what Roth coined “the best game his players have had since I’ve been here.” Parchment is off to their best start since 2017 and sitting in a three-way tie atop the SAC-Lakeshore standings and an entire group that are out to prove the doubters wrong “We have a lot of get back games and this was one of them,” Roth said I grabbed a metal chair during our pregame speech and I broke it on the ground and smacked it around and they (South Haven) think they’re the more physical team and we’re going to prove them wrong Kampen completed 16-of-24 passes for his 229 yards Taylor led the Panthers’ defense with a game-high 11 tackles and a stop for loss while Tayshawn Hartman added seven tackles and Tayshawn Hartman finished with five tackles including one stop for loss and a sack Bosma collected 97 yards rushing for South Haven Elijah Eddy added 39 yards rushing and Dominic Guzman finished tied for a game-high with 11 tackles Partly cloudy skies this evening will become overcast overnight Want to get a weekly summary of New Jersey news from dozens of trustworthy news sites and not just one who has five decades in the news business and now publishes local site 70and73.com Your browser is out of date and potentially vulnerable to security risks.We recommend switching to one of the following browsers: Account processing issue - the email address may already exist Invalid password or account does not exist Submitting this form below will send a message to your email with a link to change your password An email message containing instructions on how to reset your password has been sent to the email address listed on your account Notices are posted by 10 am Monday through Saturday Adjust Text Size: A+ A- we announce that Evelyn Kampen Carriere passed away on February 13 at the age of 35.Funeral Service will be held on Thursday As published in Winnipeg Free Press on Feb 15 Share your memories and/or express your condolences below Unfortunately with the need to moderate tributes for inappropriate content your comments may take up to 48 hours to appear citterns and dulcimers — Claire van Kampen used them all in her role as musical director on Shakespearean productions for the Globe Theatre the perfectly formed recreation of an Elizabethan playhouse on London’s Southbank."},"children":[]}]},{"name":"paragraph","children":[{"name":"text","attributes":{"value":"Yet there was nothing fusty or museum-like about the incidental music she created You want the tune that is being bandied round Bankside and the brothels and the stews that tune!’”"},"children":[]}]},{"name":"paragraph","children":[{"name":"text","attributes":{"value":"As if to prove the point she created a jazz score for the Globe’s 2001 production of "},"children":[]},{"name":"italic","children":[{"name":"text","attributes":{"value":"Macbeth"},"children":[]}]},{"name":"text","attributes":{"value":" in which the three witches danced around their cauldron as if"},"children":[]}]}]},"summary({\"maxCharCount\":200})":{"type":"json","json":[{"name":"paragraph","children":[{"name":"text","attributes":{"value":"Crumhorns and sackbuts composer at the Globe and National Theatre","seoDescription":"After convincing her husband Mark Rylance to take a part in the BBC’s Wolf Hall Van Kampen also composed the Tudor musical interludes for the seriesThe TimesMonday January 20 2025 The TimesMark Rylance and Claire van Kampen at the Vanity Fair Oscars Party in 2016MATT BARON/REX/SHUTTERSTOCKThe TimesMonday January 20 2025 the perfectly formed recreation of an Elizabethan playhouse on London’s Southbank Yet there was nothing fusty or museum-like about the incidental music she created she created a jazz score for the Globe’s 2001 production of Macbeth in which the three witches danced around their cauldron as if Registered in England No. 894646. Registered office: 1 London Bridge Street, SE1 9GF. composer and playwright had been diagnosed with cancer I would like to be emailed about offers, events and updates from The Independent. Read our Privacy notice Director, composer and playwright Claire van Kampen, the wife of actor Sir Mark Rylance has died aged 71 after being diagnosed with cancer which also marked her husband’s 65th birthday in the German town of Kassel surrounded by her family Originally trained as a concert pianist, van Kampen worked in various roles at Shakespeare’s Globe for around 20 years. Her family described her as “one of the funniest and [most] inspiring women we have ever known.” They added: “We thank her for imbuing our lives with her magic, music, laughter, and love. “Ring the bell, sound the trumpets reverie, something is done, something is beginning. One of the great wise ones has passed.” Her theater credits include composing the music for the 1989 Royal Shakespeare Company production of Hamlet starring Rylance, whom she married that same year. Later in her career, she created original scores for Broadway productions of 2000’s True West, 2008’s Boeing-Boeing and 2010 comedy La Bete, as well as adaptations of Shakespeare’s Twelfth Night and Richard III. Van Kampen also wrote the play Farinelli And The King, which saw Sir Mark star as King Philippe V of Spain and was nominated for several Olivier Awards including best new play, and a number of Tony Awards. After training at the Royal College of Music in London, she went on to have a career as a composer and performer, writing and playing for theatre, radio, television and film soundtracks and the concert hall. In 1986, she began her theater career with the Royal Shakespeare Company and with the Royal National Theatre the following year. She served as artistic associate at Shakespeare’s Globe from 1996 to 2006 when Sir Mark was the artistic director. Van Kampen later acted as a musical consultant and resident composer to the subsequent artistic director Dominic Dromgoole from 2007 to 2015. She was also a creative associate of the prestigious Old Vic theatre in London. Van Kampen was previously married to architect Christopher van Kampen, with whom she had two daughters, Juliet and Nataasha. Her youngest daughter Nataasha, a filmmaker, died aged 28 in 2012 after suffering a brain haemorrhage. Rylance is an acclaimed stage and screen star who won the Bafta TV Award for best actor for his in 2005 drama The Government Inspector and for playing Thomas Cromwell in the 2015 BBC Two mini-series Wolf Hall. In the world of film, he picked up a best supporting actor Bafta and Oscar for his role in the 2015 historical drama Bridge Of Spies. Rylance, who has also garnered Olivier and Tony awards for his theatre work, was knighted for services to theatre in the 2017 New Year Honours list. Additional reporting from the Press Association Mark Rylance and Claire van Kampen in 2022 composer and wife of Mark Rylance dies aged 71","description":"The director The theatre director passed away January 18 Oscar winner Mark Rylance paid tribute to his wife after she passed away on January 18 following a cancer diagnosis In a statement to People Magazine Rylance and his daughter Juliet van Kampen confirmed: “Claire Louise van Kampen It continued: “Claire has died of cancer on Mark Rylance’s 65th Birthday and countless beloved friends in England and America” The statement concluded: “We thank her for imbuing our lives with her magic van Kampen forged a career as a successful theatre director In 1986 she became the first female music director at the prestigious Royal Shakespeare Company later becoming the Director of Theatre Music at Shakespeare’s Globe Theatre Her accolades include the 2007 Sam Wanamaker Award Claire van Kampen (CREDIT: Bruce Glikas/Bruce Glikas/FilmMagic) She met Rylance in 1987 while working on a play together They would marry two years later in Oxford with Rylance becoming step-father to their daughters Nataasha and Juliet Nataasha van Kampen passed away in 2012 of a suspected brain haemorrhage The couple worked on many productions together and collaborated regularly when Rylance served as the first artistic director at The Globe Theatre from 1995 to 2005 The world’s defining voice in music and pop culture: breaking what’s new and what’s next since 1952. Volume 12 - 2024 | https://doi.org/10.3389/fbioe.2024.1449167 Filamentous microorganisms exhibit a complex macro-morphology constituted of branched and cross-linked hyphae Fully resolved mechanical models of such mycelial compounds rely heavily on accurate input data for mechanical properties of individual hyphae Due to their irregular shape and high adaptability to environmental factors the measurement of these intrinsic properties remains challenging To overcome previous shortcomings of microfluidic bending tests a novel system for the precise measurement of the individual bending stiffness of fungal hyphae is presented in this study microfluidic molds were fabricated with a multi-material approach enabling the creation of 3D cell traps for spore immobilization Unlike previous works applying the methodology of microfluidic bending tests the hyphae were deflected in the vertical center of the microfluidic channel eliminating the adverse influence of nearby walls on measurements This lead to a significant increase in measurement yield compared to the conventional design The accuracy and reproducibility of bending tests was ensured through validation of the measurement flow using micro-particle image velocimetry Our results revealed that the bending stiffness of hyphae of Aspergillus niger is approximately three to four times higher than that reported for Candida albicans hyphae the derived longitudinal Young’s Modulus of the hyphal cell wall yields a comparable value for both organisms The methodology established in this study provides a powerful tool for studying the effects of cultivation conditions on the intrinsic mechanical properties of single hyphae Applying the results to resolved numerical models of mycelial compounds promises to shed light on their response to hydrodynamic stresses in biotechnological cultivation which influences their expressed macro-morphology and in turn This work presents a system for microfluidic bending tests that prevents interference of the channel floor with the bending measurement the hyphae had to be positioned in the vertical center of the channel To enable true 3D structuring and at the same time achieve the necessary high resolution for manipulating single fungal hyphae the microsystems were fabricated by a two-photon polymerization (2 PP) assisted molding process a multi-material approach for the 2 PP process was required to integrate micrometer feature resolution in a structure spanning multiple millimeters the ratio of the volume flow through the growth channels towards Outlet 2 and through the serpentines towards Outlet 1 was predicted to be approximately 20 at the beginning of the loading process With each growth channel blocked by a spore while simultaneously the average absolute pressure in the loading channel increases only up to a maximum when all channels are blocked This method allowed safe loading without risking damage to the microfluidic device from overpressure caused by spores completely clogging the flow path (A) Top view of the microfluidic system layout with a zoomed view of the middle section with the growth channels The separation of the loading and measurement halves is indicated by the dashed red line different channel colors show different channel heights (B) Perspective view of the 3D-shape of a growth channel and cross-section of narrow part (C) Simplified fluidic equivalent circuit diagram used for the channel dimensioning and estimation of average chamber pressure The result was the complete negative structure of the channel system (5) we used a parylene coater (nttf coatings GmbH Germany) to deposit a 1 µm thick layer of Parylene-C on top of the structures (6) This uniformly deposited coating introduced a constant reduction of structure aspect ratio (height to width) which was accounted for in the initial design Fabrication steps of the negative mold by two-photon polymerization viewed from the side with white light microscopy images of the top view of the growth channel structures after the 63x-process and with the added channel structures after the 10x-process alignment of both system halves was carried out in a mask aligner (EVG 420 Permanent bonding was achieved through oxygen plasma treatment the channel system and the cell traps where identical for both configurations except for the measurement chamber height which was doubled Schematic cross sectional view of the two system configurations with the hypha either (A) on the floor (floor configuration) or (B) in the vertical center (center configuration) of the measurement chamber The bond line for both systems is depicted in red Parameters for femtosecond laser ablation processes while for the measurement flow we chose a 2.5 mL glass syringe (SETonic GmbH (A) Chip holder used for fixating and interfacing of the chip during fluidic experiments (B) Set up for bending experiments with a connected syringe pump in the foreground and the chip holder installed on the stage of the inverted microscope in the background In order to enhance comprehension of the fluid-induced forces we conducted a CFD simulation of the measurement flow around the hypha using the software ANSYS Fluent (V We used a model of a 500 µm long section of the measurement chamber with a rectangular profile and a rigid hypha positioned in the vertical channel center We further selected velocity inlet and outflow as the inlet and outlet boundary conditions The walls were modelled without slip and a symmetry condition was applied at the symmetry plane of the geometry to reduce computational cost The geometry was discretized using a tetrahedral mesh with refinement of mesh size on and near the surface of the hypha A fully laminar flow model was used for the calculations The results for the individual z-positions were then stacked to obtain the flow field The A. niger strain SKAn1015 (Zuccaro et al., 2008) was used as the organism in this study due to its extensive history of research on various aspects of its cultivation and biology (Priegnitz et al., 2012) spores were collected from a sporulating agar plate using a cotton swab and then spread onto a fresh agar plate The newly inoculated agar was then incubated at 37°C for 4 days until excessive sporulation was visible the plate was flooded with a 0.9% NaCl solution and gently scrubbed with another cotton swab to loosen the spores a filtration step was performed using Miracloth filtration paper (Merck pore size 22–25 µm) to remove any mycelial compounds or other unwanted contaminants from the agar plate The spore suspension obtained was stored at 4°C for a maximum of 7 days before being used in experiments a minimal medium containing 22 g L−1 glucose as carbon source 0.5 g L−1 MgSO4·7H2O 20 mL L−1 salt solution (26.1 g L−1 KCl 74.8 g L−1 KH2PO4 and 250 g L−1 NaNO3) and 1 mL L−1 trace element solution (10 g L−1 EDTA 4.4 g L−1 ZnSO4 7H2O 1 g L−1 MnCl2 4H2O 0.315 g L−1 CuSO4 5H2O 2.5 g L−1 FeSO4·7H2O 0.32 g L−1 CoCl2 6H2O 1.47 g L−1 CaCl2 2H2O CaCl2·2H2O and 0.22 g L−1 (NH4)6Mo7O24 4H2O) was used The carbon source and the other ingredients were sterilyzed separately in an autoclave before mixing the whole solution was adjusted to a pH of 3 with HCl (3 M) together with examples of valid and invalid hyphae following the criteria Substituting this velocity profile accordingly into the analytical 2D solution for the force per unit length for the hypha in the floor configuration it is given by the hyphal radius r and the fluidic resistance per unit length Rf (formula see Supporting Informations (SI)) The latter also includes the fluid’s dynamic viscosity the theoretical flow profile has been substituted into the analytical 2D solution for an infinite cylinder submerged in a confined flow in the middle of two parallel plates to yield the force per unit length as As the span of this additional load component is small compared to the hyphal length (L≫r) this was taken into account as an additional point load Ftip at the tip Based on the simulation results its magnitude was estimated as to effectively double the force per unit length along the hyphal tip. The integral representation is also shown in Figure 5B Figure 5. (A) Schematic isometric view of the open channel with a hypha in the center configuration and the Poiseuille velocity field in the background. (B) Comparison of the force per unit length as calculated with Equation 2 and extracted from CFD simulation results, normalized with the maximum of the analytically calculated value. The integral representation of the estimated Ftip calculated with Equation 3 is added as the hatched area The Euler-Bernoulli beam theory for a beam with constant cross section was applied to calculate the hyphal bending stiffness from the measured tip displacement and the calculated hydrodynamic loads. For this purpose the initial angle α0 and the deflected angle α1 of every individual hypha were considered, see Figure 6A The real initial and deflected shape of each hypha is approximated by a straight connection line reaching from its base to its tip the hypha is bent by the hydrodynamic load f*y which compared to fy is diminished by the factor cosα1 due to the force not acting perpendicular to the hypha As the vertical displacement (perpendicular to the hyphal main axis) is addressed in the Euler-Bernoulli beam theory the initial tilt angle α0 has to be considered to yield the vertical tip deflection from measurements This is achieved by multiplying the measured displacement in x-direction by cosα0−1 The hypha is moreover assumed to be constant in cross-section the differential equation for the hypha bending problem according to the Euler-Bernoulli beam theory The resulting relation for the total tip deflection was used to deduce the bending stiffness from observed tip deflections, where Ftip was calculated according to Equation 3 with f=f* Not considering the tip force leads to an under estimation of the bending stiffness of more than 12% for L = 40 μm sharply increasing with decreasing hyphal length To ensure a vanishing relative error introduced by using the linearized small deflection Euler-Bernoulli beam theory we only considered data points with a relative tip deflection of wmax/L≤0.25 (A) Schematic top view of a hypha without flow (dark blue) and in bent state (light blue) defining parameters used for data analysis (B) Schematic free body diagrams for the loads considered: the analytical force per unit length on a cylinder (above) and the estimated tip force (below) meaning by average it took a hypha about 7.5 h to reach the measurement chamber after germination or entering the growth channel Light microscopy images of three growth channels in a system in floor configuration showing immobilized spores after 0 The red arrow marks the position of the hyphal tips growing through the channels towards the measurement area where the maximal achieved force per unit length at y=w/2 is approximately 160 pN μm−1 in floor configuration and 380 pN μm−1 in center configuration While f is approximately proportional to u for the center configuration in floor configuration it reaches a plateau after about w/4 due to the proportionality to the wall velocity gradient δuδz|z→0 The portrayed load progression for the center configuration stems from the chamber cross section with double the height as compared to the floor configuration which results in half of the average velocity at the same flow rate We chose this because these are the actual geometrical parameters of the systems used Comparing the measurement results close to the entrance and at the exit of the measurement chamber showed no significant variation of velocity along the flow direction (data not shown) thus any possible influence of the growth channels on the flow field along the length of the measurement chamber were assumed to be negligible Results from µPIV measurements in the measurement chamber (A) Full measured velocity field (x-component) over the cross-section of the measurement chamber half way between in- and outlet C) provide thereof extracted profiles vertically (V) and horizontally (H) through the center (D) Progression of the distributed force calculated from the fit for both configurations Error bars indicate the standard deviation of the average along x-direction we obtained a slightly higher mean bending stiffness of 18.3 ± 8.7 μN μm2 as compared to the floor configuration the displayed results were obtained over the course of two cultivations while for the center configuration all displayed data was obtained from a single cultivation experiment with only one microfluidic chip This vastly improved yield for the center configuration reflects the absence of chamber floor adhesion due to the symmetrical measurement setup None of the analyzed hyphae showed significant hysteresis when returning to their initial shape after the measurement flow vanished The reason for the approximately 66% increase in measured bending stiffness in between the configurations could be a systematic underestimation of the flow induced forces in floor configuration due to the hyphae deviating from the ideal case of full contact with the underlying surface over its length flow between the hypha and surface might occur an overestimation of the forces due to the hyphae growing away from the center plane of maximum velocity (z=h/2) would in turn lead to an overestimation of the bending stiffness Results from bending experiments for floor and center configuration (A) Frames recorded during a bending experiment with increasing flow rates in floor configuration and (B) values of tip deflection extracted from these frames versus the applied volume flow rate scaled by the equilibrium tilt angle The fit used to extract kb is indicated by the red dash-line D) show the respective frames and plotted data for a hypha in center configuration the cell traps and growth channels are located above the image section shown with the growth channel exits visible at the very top (E) Box plots of the measured bending stiffness of all analyzed hyphae in floor (nfloor = 4 from two cultivations) and center configuration (ncenter = 22 from one cultivation one outlier with kb = 45 μN μm2 not shown) Scale bars = 10 µm niger hyphae that their cell wall’s AFM indentation modulus increases significantly with cultivation time An increase in cell wall elasticity modulus with cell wall age would then lead to a proportional increase in bending stiffness of these hyphal segments Our model assumes constant bending stiffness and thus cell wall elasticity and cross section so the measured average bending stiffness in this case would increase as seen in the hyphae with longer exposed lengths the microfluidic bending test in center configuration is a promising tool for parallelized studies of the mechanical properties of filamentous cells as evidenced by the significant improvement in the measurement yield per experiment compared to the conventional floor configuration design This enables statistically significant results on the influence of environmental factors on the bending stiffness of the cells within singular on chip cultivation measurements the presented system demonstrates the capability to measure cell wall properties without the need for any further handling of the live cells enabling the non-invasive monitoring of mechanical markers during cultivation Future work will aim at the application of the methodology to different pharmaceutically relevant species such as actinomycetes for a better understanding of the cultivation process Another focus will be the integration of sensors into the microfluidic system such as a passive 2 PP-fabricated flow sensor to aid data analysis as well as the monitoring of the cultivation conditions inside the microsystem to be able to study the individual growth morphology in more detail and to incorporate it into the data analysis we aim at integrating live monitoring of the 3D-shape during tests by combining our implemented protocol with confocal microscopy our presented work provides a basis for resolved and detailed on-chip study of filamentous cultures and their mechanics The raw data supporting the conclusions of this article will be made available by the authors The author(s) declare that financial support was received for the research The authors gratefully acknowledge the financial support for parts of the work provided by the German Research Foundation (DFG) in the priority programme “DiSPBiotech–Dispersity structural and phase modifications of proteins and biological agglomerates in biotechnological processes” (SPP 1934 We acknowledge the support by the Open Access Publication Funds of the Technische Universität Braunschweig The authors would like to express their gratitude to Mathias Müsken of the Central Facility for Microscopy (ZEIM) of Helmholtz Center for Infection Research (HZI, Braunschweig, Germany) for conducting TEM measurements of hyphal cross sections. The authors used deepl.com in order to improve the quality of the written English the authors reviewed and edited the text as needed and take full responsibility for content of the publication The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest The author(s) declared that they were an editorial board member of Frontiers This had no impact on the peer review process and the final decision All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fbioe.2024.1449167/full#supplementary-material Fungal foraging behaviour and hyphal space 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two-photon polymerization of microfluidic mold Received: 17 June 2024; Accepted: 11 October 2024;Published: 01 November 2024 Copyright © 2024 Brinkmann, Schrader, Meinen, Kampen, Kwade and Dietzel. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use distribution or reproduction in other forums is permitted provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited in accordance with accepted academic practice distribution or reproduction is permitted which does not comply with these terms *Correspondence: Steffen Brinkmann, cy5icmlua21hbm5AdHUtYnJhdW5zY2h3ZWlnLmRl Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. 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It concluded with a poetic tribute: "Ring the bell Claire van Kampen and husband Mark Rylance Van Kampen trained at the Royal College of Music in London studying music theory and piano with a specialisation in 20th century music performance She began her theatre career in 1986 with the Royal Shakespeare Company joining the Royal National Theatre the following year she served as artistic associate at Shakespeare's Globe during Sir Mark's tenure as artistic director She later continued her connection with the Globe as musical consultant and resident composer under artistic director Dominic Dromgoole from 2007 to 2015 She also composed the music for BBC series Wolf Hall as well as music for her husband's performance in the 1989 Hamlet Mark Rylance and Claire van Kampen attend The BFG (Le Bon Gros Geant - Le BGG) premiere during the 69th annual Cannes Film Festival at the Palais des Festivals in 2016 She was also appointed as a creative associate at London's prestigious Old Vic theatre died in 2012 at the age of 28 after suffering a brain haemorrhage It didn't take long for social media users to flood platforms with heartfelt tributes co-artistic directors at Royal Shakespeare Company It read: "We are deeply saddened to hear of the passing of Claire van Kampen today.Claire was a true genius not only in her music but also in her understanding of Shakespeare and of the relationship between actor music and silence in so many forms of storytelling "Her score for Pericles this season was a thing of wonder and beauty which captivated audiences at the Swan and in Chicago alike Claire van Kampen died on Saturday on her husband Mark Rylance's birthday "The world is a far more beautiful place for having had her in it One fan penned: "Heartbroken to hear of Claire van Kampen’s passing "So sad to hear about claire van kampen she was super talented and will be missed by so many sending love to her fam and everyone who knew her," another shared Someone else posted: "Sending love to mark rylance & his stepdaughter juliet today as his wife/her mom losing a loved one on any given day is heartbreaking but to lose your wife on your birthday is awful." 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