Metrics details The prevailing assumption is that all the molecular events required for piRNA-directed DNA methylation occur after the engagement of MIWI2 We find that SPIN1 expression precedes that of both SPOCD1 and MIWI2 H3K9me3 and SPIN1 before the initiation of piRNA-directed DNA methylation We generated a Spocd1 separation-of-function allele in the mouse that encodes a SPOCD1 variant that no longer interacts with SPIN1 We found that the interaction between SPOCD1 and SPIN1 is essential for spermatogenesis and piRNA-directed DNA methylation of young LINE1 elements We propose that piRNA-directed LINE1 DNA methylation requires a developmentally timed two-factor authentication process The first authentication is the recruitment of SPIN1–SPOCD1 to the young LINE1 promoter and the second is MIWI2 engagement with the nascent transcript independent authentication events underpin the precision of piRNA-directed LINE1 DNA methylation piRNA-directed transposon methylation requires precision Failing to methylate every active transposon would be detrimental to the genomic integrity of the germline but aberrant off-target methylation could result in germline-transmitted epimutations piRNAs endow MIWI2 with the specificity to identify active transposon loci and whether other mechanisms contribute to identifying active transposons and the exacting precision of the pathway remains unknown AlphaFold2 co-folding prediction of the interaction between SPIN1 (Q61142) and SPOCD1 (B1ASB6; only amino acids 326–348 are shown) Crosslinking mass spectrometry of mouse SPOCD1 fragment 1b (amino acids 203-409) with mouse SPIN1 (amino acids 49–262) Phylogenetic tree from ray-finned fishes to mammals showing the presence of SPOCD1 and SPIN1 in the indicated animal clades AlphaFold2 prediction of SPOCD1 from Anolis carolinensis (an anole lizard XP_031752218.1) and Latimeria chalumnae (coelacanth TFIIS-M domain and SPIN1-interacting β-hairpin are highlighted Multiple sequence alignment of the SPOCD1 SPIN1-interacting β-hairpin region from different species Numbering for mouse SPOCD1 is shown above the sequences and secondary-structure elements of mouse SPOCD1 are shown below Sequences are coloured according to sequence identity Representative Coomassie gel image of n = 3 co-precipitation experiments with the indicated recombinant SPOCD1 from different species with mouse SPIN1 SPIN1 (green) and DAPI (blue) staining of wild-type fetal testis sections from the indicated developmental time points Images are representative of n = 3 biological replicates Volcano plot showing enrichment (log2(mean label-free quantification ratio of anti-HA immunoprecipitates from Spocd1HA/HA/wild-type)) and statistical confidence (−log10(P-value of two-sided Student’s t-test)) of proteins co-purifying with HA-SPOCD1 from E14.5 fetal testes; n = 3 H3K9me3 and SPIN1 from E14.5 fetal germ cells H3K9me3) and three (SPIN1) biological replicates metaplot and heatmaps of signal over elements of different transposon families (e) are shown as well as young and old copies in the L1Md_T (f) and L1Md_A (g) families Columns adjacent to the heatmaps show statistically significant peaks called for SPIN1 and the indicated histone modifications the overlap of H3K4me3 and H3K9me3 peaks with SPIN1 peaks is significant for L1Md_A (P = 0.0099 Z-score = 2,007) by one-tailed permutation tests enrichment of overlapping H3K4me3 and H3K9me3 peaks with SPIN1 peaks is significantly different between young and old L1Md_A (adjusted P < 2.2 × 10−16) and L1Md_T (adjusted P < 2.2 × 10−16) copies as observed by two-tailed Fisher’s exact test charts show overlap analysis of H3K4me3 and H3K9me3 peaks (h) and SPIN1 peaks (i) with the indicated genomic features P-values and Z-scores from one-tailed permutation tests to assess the statistical significance of overlaps of CUT&TAG peaks with LINE1 elements are shown a, Representative western-blot analyses of n = 3 immunoprecipitations of the mouse wild type and eight SPOCD1 alanine mutations (8 Ala mut) with SPIN1 in HEK 293 T cells. For whole-blot source data, see Supplementary Fig. 1 Representative Coomassie gel image of n = 3 co-precipitation experiments with the indicated recombinant proteins Representative images of E16.5 gonocytes from n = 3 wild-type (WT) and Spocd1ΔSPIN1 mice stained for DNA (blue) and SPOCD1 (c) Number of embryos per plug fathered by studs with the indicated genotype mated to wild-type females from n = 6 wild-type (15 plugs in total) and n = 6 Spocd1ΔSPIN1 studs (12 plugs) Testis weight of adult mice with the indicated genotype from n = 8 wild-type and n = 8 Spocd1ΔSPIN1 mice a representative image of testes from wild-type (left) and Spocd1ΔSPIN1 (right) mice P-values in f and g were determined by unadjusted two-sided Student’s t-test Representative images of PAS and haematoxylin-stained testes sections of wild-type and n = 5 Spocd1ΔSPIN1 adult mice with different types of spermatogenic arrest observed in the tubules of the Spocd1ΔSPIN1 testes indicated The percentage of each type of tubule is noted alongside Adult testis sections stained for the DNA damage marker γH2AX (red) (i) and apoptotic cells (red) by TUNEL assay (j) from wild-type and Spocd1ΔSPIN1 mice (representative of n = 3 mice per genotype for γH2AX and n = 2 wild-type plus n = 3 Spocd1ΔSPIN1 mice for TUNEL) Representative testis sections of n = 3 wild-type Spocd1ΔSPIN and Spocd1−/− mice stained red for the LINE1 ORF1p (a) or IAP GAG protein (b) RNA-seq heat maps showing fold changes in expression relative to wild type for the ten most upregulated LINE1 and ERVK transposable elements in Spocd1−/− P20 testes (n = 3 from each genotype) ***P < 0.001 of Bonferroni-corrected two-sided Wald’s test assuming n-binominal distribution Only significant differences (P < 0.05) are shown Genomic CpG methylation analysis of P14 undifferentiated spermatogonia from wild-type (n = 6) Spocd1ΔSPIN (n = 4) and Spocd1−/− mice (n = 3) Percentages of CpG methylation levels of the indicated genomic features (with genic promoter and CpG island (CGI) regions defined as those not overlapping transposable elements and intergenic regions as those not overlapping transposable elements or genes) or transposable elements (not overlapping genes) are shown as box plots Boxes represent interquartile range from the 25th to the 75th percentile and whiskers show the data range of the median ± twice the interquartile range Significant differences (P < 0.05 of Bonferroni-corrected two-tailed Student’s t-tests) of Spocd1ΔSPIN (n = 4) and Spocd1−/− (n = 3) samples to wild-type (n = 6) are indicated Metaplots of mean CpG methylation over the indicated transposable element **P = 0.01–0.001 and ***P < 0.001 for Bonferroni-corrected two-tailed Student’s t-tests comparing the average CpG methylation of the promoter region to wild type for Spocd1ΔSPIN1 (red) and Spocd1−/− (blue) Correlation analysis of mean CpG methylation loss relative to the wild type for individual transposable elements of the indicated LINE1 and ERVK families in relation to their divergence from the consensus sequence in Spocd1ΔSPIN spermatogonia How SPOCD1 is recruited to IAPs remains unknown but we speculate that another SPOCD1-associated protein could mediate this recruitment through the recognition of a distinct chromatin signature or sequence motif The different mechanisms in LINE1 and IAPs reveals an unexpected complexity in the pathway The prevailing notion is that all the molecular events required for piRNA-directed DNA methylation occur after the engagement of the piRNA–MIWI2 ribonucleoprotein complex with the nascent transcript we demonstrate that multiple independent and developmentally choreographed events are required for LINE1 piRNA-directed DNA methylation Our revised model posits that the recruitment of SPIN1–SPOCD1 through chromatin modification to young LINE1 elements constitutes a first licensing step The engagement of MIWI2 with the nascent transcript is the second licensing event and triggers DNA methylation we propose that a two-factor authentication system ensures the precision of LINE1 piRNA-directed methylation Male fertility was assessed by mating studs to Hsd:ICR (CD1) wild-type females and counting the number of pups born for each plugged female animal tissue samples were collected from one or more litters and allocated to groups according to genotype No further randomization or blinding was applied during data acquisition and analysis Animals were maintained at the University of Edinburgh in accordance with the regulation of the UK Home Office or at the Institute for Molecular Biology in Mainz in accordance with local and European animal-welfare laws Ethical approval for the UK mouse experimentation has been given by the University of Edinburgh’s Animal Welfare and Ethical Review Body and the work done under licence from the UK Home Office Animal experiments done in Germany were approved by the ethical committees on animal care and use of the federal states of Rheinland-Pfalz Duke University) 1:500; anti-γH2AX (Bethyl Laboratories) 1:500; anti-MIWI2 (a gift from R Université de Genève) 1:500; anti-SPOCD1 rabbit serum rb175 1:500 (O’Carroll laboratory antibody); anti-SPIN1 (Cell Signaling Technologies) 1:500 (of a custom preparation of 1.1 μg μl−1 in PBS) Images were taken on a Zeiss Observer or Zeiss LSM880 with an Airyscan module Images acquired using the Airyscan module were deconvoluted with the Zeiss Zen software ‘Airyscan processing’ with settings 3D and a strength of 6 ImageJ and Zeiss Zen software were used to process and analyse the images cells were washed twice with PBS and resuspended in 1 ml lysis buffer (IP buffer: 150 mM KCl supplemented with 1× protease inhibitors (cOmplete ULTRA EDTA-free Roche) with 37 units per ml benzonase (Millipore)) and lysed for 30 min The lysate was cleared by centrifugation for 10 min at 21,000g Cleared lysate (800 μl) was incubated with 20 μl of anti-HA beads (Pierce) that had been calibrated in lysis buffer and incubated for 1 h at 4 °C on a rotating wheel The beads were washed four times with lysis buffer Immunoprecipitates were eluted at 50 °C for 10 min in 20 μl 0.1% sodium dodecyl sulphate (SDS) Lysates and eluates were run on a 4–12% bis–tris acrylamide gel (Invitrogen) and blotted onto a nitrocellulose membrane (Amersham Protran 0.45 NC) according to standard laboratory procedures The membrane was blocked with blocking buffer (4% (w/v) skimmed milk powder (Sigma-Aldrich) in PBS-T (phosphate buffered saline 0.1% Tween-20)) and subsequently incubated for 1 h with primary antibodies (anti-HA (C29F4s anti-SPOCD1 rabbit serum rb175 (O’Carroll laboratory antibody) 1:500 or anti-α-Tubulin (T9026 Sigma-Aldrich) 1:1,000) in blocking buffer The anti-α-tubulin staining was used as loading control on the same blot as the experimental staining the membrane was incubated with secondary antibodies (IRDye 680RD donkey anti-rabbit or IRDye 800CW donkey anti-mouse It was washed three times for 10 min in PBS-T and imaged on a LI-COR Odyssey CLx system Exposure of the entire images was optimized in Image Studio Lite (LI-COR) and areas of interest were cropped for presentation GST-tagged mouse SPOCD1 fragments (amino acids 203–409) Latimeria SPOCD1 fragments (XP_014348336.1 amino acids 510–1009) and His-tagged SPIN1 (amino acids 49–262) were cloned in a pET-based backbone Proteins were expressed in Escherichia coli BL21 (DE3) Bacteria were grown in 2xTY media at 37 °C until an optical density of 0.8 was reached the bacteria were induced with 1 mM IPTG and grown for another 14–16 h Cells were collected and pellets were stored at −80 °C until purification The pellets were resuspended in 50 ml lysis buffer (20 mM Tris-HCl Roche cOmplete EDTA-free Protease Inhibitor Cocktail 0.01 mg ml−1 DNaseI (Sigma) and 2 mM AEBSF (Pefabloc) for SPIN1 0.01 mg ml−1 DNaseI (Sigma) and 2 mM AEBSF (Pefabloc) for SPOCD1) and cells were lysed with the Constant systems 1.1 kW TS cell disruptor at 25 kPSI The cleared lysate was used to load on a cOmplete His-Tag Purification Column (Roche) for SPIN1 or incubated with 7 ml glutathione sepharose high-performance beads (Cytiva) for SPOCD1 calibrated in the respective buffer Elution from column/beads with increasing (2.5–500 mM) imidazole gradient for SPIN1 or GST elution buffer containing 20 mM reduced glutathione for SPOCD1 The fractions of interest were pooled and dialysed overnight in 20 mM Tris-HCl The SPIN1 construct was cleaved with GST–3C protease (made in our lab) overnight The SPOCD1 constructs were concentrated and stored at −80 °C until used SPIN1 was further purified by ion exchange with a gradient of 100–1,000 mM NaCl (Resource Q Cytiva) and size-exclusion chromatography (HiLoad 16/600 Superdex 200 pg the protein was concentrated and stored at −80 °C until used starting from H3Δ1–31T32C C110A constructs that also contained the required H3X and H3Y mutations H3X was used for H3K4me3 and H3Y for H3K9me3 A biotinylated 209-bp DNA fragment containing the 601 nucleosome positioning sequence was generated by PCR and purified by ion-exchange chromatography on a HiTrap Q column followed by ethanol precipitation Mononucleosomes were then assembled from histone octamers and 601 DNA by gradient dialysis Nucleosome assembly was verified by native gel electrophoresis on 6% acrylamide gels in 0.5× TGE buffer (12.5 mM Tris After incubation with recombinant proteins beads were washed three times with high-salt pull-down buffer (as above but with 350 mM NaCl) for 5 min Nucleosomes and bound proteins were eluted by boiling in 1.5× SDS sample buffer (95 mM Tris HCl Binding was analysed by western blotting with antibodies against His tag (Sigma H1029 Antibodies against histone H3 (Abcam ab176842 H3K4me3 (Cell Signaling) 1:2,000 and H3K9me3 (Abcam ab176916) 1:1,000 were used to verify nucleosome loading and modification state For analytical size-exclusion chromatography 125 μg SPIN1 and/or 500 μg mouse GST–SPOCD1-F1b were used for each run Proteins were diluted in 250 μl size-exclusion chromatography buffer (20 mM HEPES 1 mM DTT) and injected on a Superdex 200 10/300 GL column loaded on an SDS–PAGE gel and visualized by Coomassie staining IP-MS of SPOCD1–HA from Spocd1HA/+ E14.5 fetal testis using 50 μl of anti-HA beads (Pierce, 88837) was done as previously described4 with a reduced number of 25 testes per replicate Wild-type fetal testes were used as controls To purify foetal germ cells for CUT&Tag analysis, E14.5 testes were dissected from embryos carrying the Oct4eGFP allele34 A single cell suspension was obtained by sequential treatment with 100 µl collagenase solution at 37 °C for 8 min (10 units of collagenase A (Sigma-Aldrich 10103578001); 2× NEAAs (Gibco); 2× Na-pyruvate (Gibco); 25 mM HEPES–KOH pH 7.5) and 200 µl TryPLE Express (Gibco) at 37 °C for 5 min with gentle flicking and pipetting of the solution to aid dissociation Digestion was neutralized by 70 µl prewarmed FBS and cells were collected by spinning at 600g for 4 min at room temperature followed by two washes in FACS buffer (1× PBS; 2 mM EDTA 10% FBS; 2 µg ml−1 DAPI) and filtering (Corning Cell sorting was done on an Invitrogen Bigfoot using a 100 μm nozzle and gating for DAPI-negative (live) OCT4–eGFP-positive (germ cells) populations into collection tubes containing 100 µl 1× PBS For EM-seq, CD9+ spermatogonia were sorted from P14 testes as described previously52 using Fc block (eBioscience lot 2297433) 1:50; biotin-conjugated anti-CD45 (eBioscience and biotin-conjugated anti-CD51 (Biolegend lot B308465) 1:100 anti-CD9APC (eBioscience Cells were sorted into DMEM media on a BD Aria II sorter pelleted for 5 min at 500g and snap frozen in liquid nitrogen For gating strategies, see Supplemental Fig. 2 using pA–Tn5 at a 1:400 dilution (Diagenode C01070001) and 15 PCR cycles of library amplification Libraries were cleaned up by magnetic bead-based solid-phase separation and assessed on a Tapestation (Agilent) Antibodies and dilutions used for CUT&Tag were rabbit IgG control (Abcam and guinea pig anti-rabbit IgG (Antibodies Online Pooled libraries were sequenced using paired-end 150 bp on a NextSeq 2000 instrument (Illumina) using the following parameters: -bs 1 --normalizeUsing CPM —exactScaling --ignoreForNormalization MT Log2 enrichment profiles of CUT&Tag samples over IgG controls were generated with deepTools bamCompare using the following parameters: -bs 1 --normalizeUsing CPM --exactScaling --ignoreForNormalization MT --scaleFactorsMethod None Monomers associated with inert promoters (subtypes 6 and 2) were removed from the analysis The central regions of repetitive elements were length-normalized to 5 kb with flanking regions ±2 kb from the start and end positions Heatmaps and profile plots show data in consecutive 10b bins with regions subdivided by elements and arranged in descending order of total enrichment across all samples Histology experiments on mouse samples were done as previously described4 TUNEL assay experiments were done as previously described4 two-tailed Student’s t-tests were used to compare the differences between groups and adjusted for multiple testing using Bonferroni correction where indicated Averaged data are presented as mean ± s.e.m. No statistical methods were used to predetermine the sample size The experiments were not randomized and the investigators were not blinded to allocation during experiments and outcome assessment Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article The scripts used for the EM-seq and RNA-seq analysis are available from github at https://github.com/rberrens/SPOCD1-piRNA_directed_DNA_met, and the scripts used for ChIP and CUT&Tag analysis are available from github at https://github.com/swebb1/heep-et-al_2024 PIWI-interacting RNAs: small RNAs with big functions The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing SPOCD1 is an essential executor of piRNA-directed de novo DNA methylation Zoch, A. et al. C19ORF84 connects piRNA and DNA methylation machineries to defend the mammalian germ line. Mol Cell https://doi.org/10.1016/j.molcel.2024.01.014 (2024) Structural mechanism of bivalent histone H3K4me3K9me3 recognition by the Spindlin1/C11orf84 complex in rRNA transcription activation Identification of autonomous IAP LTR retrotransposons mobile in mammalian cells Jr A novel active L1 retrotransposon subfamily in the mouse Developmentally regulated piRNA clusters implicate MILI in transposon control Meiotic catastrophe and retrotransposon reactivation in male germ cells lacking Dnmt3L MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline Transcription of IAP endogenous retroviruses is constrained by cytosine methylation The diverse roles of DNA methylation in mammalian development and disease A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice Two waves of de novo methylation during mouse germ cell development Nucleolar protein Spindlin1 recognizes H3K4 methylation and stimulates the expression of rRNA genes Distinct mode of methylated lysine-4 of histone H3 recognition by tandem tudor-like domains of Spindlin1 Active genes are tri-methylated at K4 of histone H3 Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins Highly accurate protein structure prediction with AlphaFold Highly accurate protein structure prediction for the human proteome Broad heterochromatic domains open in gonocyte development prior to de novo DNA methylation Role of the Dnmt3 family in de novo methylation of imprinted and repetitive sequences during male germ cell development in the mouse Efficient low-cost chromatin profiling with CUT&Tag A tudor domain protein SPINDLIN1 interacts with the mRNA-binding protein SERBP1 and is involved in mouse oocyte meiotic resumption The DNA methyltransferase DNMT3C protects male germ cells from transposon activity encoding a DNA methyltransferase homolog required for meiosis and transposon repression in the mouse male germline DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes A transit-amplifying population underpins the efficient regenerative capacity of the testis One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering Oct4 expression is not required for mouse somatic stem cell self-renewal A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming G9a co-suppresses LINE1 elements in spermatogonia ColabFold: making protein folding accessible to all PyMOL Molecular Graphics System v.1.8 (Schrodinger scalable generation of high-quality protein multiple sequence alignments using Clustal Omega Jalview Version 2−a multiple sequence alignment editor and analysis workbench ChromID identifies the protein interactome at chromatin marks A synthetic biology approach to probing nucleosome symmetry The histone H3.1 variant regulates TONSOKU-mediated DNA repair during replication Trimmomatic: a flexible trimmer for Illumina sequence data Li, H. Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM. Preprint at https://arxiv.org/abs/1303.3997 (2013) The Sequence Alignment/Map format and SAMtools deepTools2: a next generation web server for deep-sequencing data analysis Subtype classification and functional annotation of L1Md retrotransposon promoters SeqPlots – interactive software for exploratory data analyses pattern discovery and visualization in genomics Wickham, H. Welcome to the Tidyverse. J. Open Source Softw. https://doi.org/10.21105/joss.01686 (2019) Defective germline reprogramming rewires the spermatogonial transcriptome The nf-core framework for community-curated bioinformatics pipelines regioneR: an R/Bioconductor package for the association analysis of genomic regions based on permutation tests Gaspar, J. M. Improved peak-calling with MACS2. Preprint at bioRxiv https://doi.org/10.1101/496521 (2018) Software for computing and annotating genomic ranges pyGenomeTracks: reproducible plots for multivariate genomic datasets The Perseus computational platform for comprehensive analysis of (prote)omics data Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 Download references This research was supported by funding from Wellcome to D.O’C (095021 and 200885); the Wellcome Centre for Cell Biology (203149 and multi-user equipment grants 108504 and 092076); funding for the Wellcome Discovery Research Platform for Hidden Cell Biology (226791); and support from the microscopy proteomics and bioinformatics cores of the Wellcome Discovery Research Platform for Hidden Cell Biology was funded by a German Research Foundation fellowship (DFG award ZO 376/1-1); J.B were funded by a German Research Foundation collaborative research centre grant (DFG grant SFB 1361 Work in P.V.’s lab was supported by Wellcome (104175/Z/14/Z) and the UK Biotechnology and Biological Sciences Research Council (BBS/E/B/000C0421) are funded by the Darwin Trust of Edinburgh This work used the University of Edinburgh Protein Production Facility (EPPF) the Wellcome Centre for Cell Biology’s Centre Optical Instrumentation Laboratory (COIL) proteomics and bioinformatics core platforms and the Centre for Regenerative Medicine’s FACS facility We also thank staff at the EMBL GeneCore facility in Heidelberg for preparing the methyl-seq libraries and sequencing all libraries; S Nick at IMB flow-cytometry core facility for assistance with operating the Bigfoot cell sorter instrument (project number 511658729); and M Heinen at the IMB protein production core facility for the recombinant Tn5 protein fusions These authors contributed equally: Ansgar Zoch Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute) execution and analysis of most of the experiments did the immunofluorescence and IP-MS experiments did the CUT&RUN and CUT&Tag experiments under the guidance of J.B did the bioinformatic analysis of the EM-seq generated site-specifically modified histones and designed and performed the nucleosome pull-down experiments did the molecular-biology and histology experiments analysed the crosslinking-mass spectrometry data contributed to analyses and experimental design The authors declare no competing interests reviewer(s) for their contribution to the peer review of this work Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations HA (red) and DAPI (blue) staining of E16.5 foetal testis sections from Spocd1HA/+ mice treated with PBS or RNase A prior to fixation HA (red) and DAPI (blue) staining of E16.5 foetal testis sections from E16.5 Miwi2−/−;Spocd1HA/+ and Miwi2+/−;Spocd1HA/+ mice SPIN1 (green) and DAPI (blue) staining of E16.5 Miwi2+/− and Miwi2−/− E16.5 foetal testis sections (c) shows a zoom-in of the cell highlighted with a dashed rectangle in (d) Images of (a-d) are representative of n = 3 biological replicates sequences are coloured according to sequence identity Numbering above according to mouse sequence Panels show H3K4me3 (a) and H3K9me3 (b) ChIP-seq signal in reads per million (RPM) over young and old elements within the indicated LINE1 family Metaplot and heat maps of indicated CUT&Tag signal of H3K4me3 H3K9me3 and SPIN1 over young and old L1MD_F elements Columns adjacent to the heatmaps show peaks called for SPIN1 and the indicated histone modifications Data depicts element plus adjacent 2 kb for each of the transposon families indicated Genome snapshots showing datatracks of CUT&Tag signal of H3K4me3 H3K9me3 and SPIN1 over selected genome regions containing a young L1Md_A Enrichment of overlapping H3K4me3 and H3K9me3 peaks with SPIN1 peaks is not significantly different between young and old L1Md_F copies as observed by a two-tailed Fisher’s exact test Representative images of sections from n = 3 wild-type foetal testis stained for SPIN1 (green) and DAPI (blue) from indicated timepoints Cell shown in (a) is highlighted with a white box in (b) a, Representative western blot analyses of n = 3 anti-HA immunoprecipitations of the HA epitope-tagged mouse wild-type, SPOCD1 8 alanine mutated proteins or GFP control with FLAG-tagged DNMT3L in HEK 293 T cells. For whole blot source data, see Supplementary Fig. 1 Schematic representations of the mouse Spocd1 locus and encoded 1015 amino acid protein are shown sgRNA used for generation of the Spocd1ΔSPIN1 allele and adjacent PAM site are indicated Schematic of CRISPR targeting strategy showing the location of single-stranded oligo DNA donor (ssODN) and homology arms (HA) used and sequencing trace of the part of Spocd1ΔSPIN1 exon 4 harbouring the mutation sites a 30 bp sequence creating the 8 alanine mutation is highlighted in red Representative image of genotyping result for n = 3 Spocd1+/+ Representative images of E16.5 gonocytes from n = 3 Spocd1ΔSPIN1 and wild-type control mice stained for SPOCD1 (e) Supplementary Figures containing uncropped scans of the western-blot experiments shown in Figs and the FACS gating strategy for sorting fetal germ cells and undifferentiated spermatogonia (Supplementary Fig Further data relating to crosslinking mass spectrometry data Download citation DOI: https://doi.org/10.1038/s41586-024-07963-3 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing newsletter — what matters in science Portugal’s CTM Mirandela lost a long-distance duel in Round 3 of the Europe Cup Women but thanks to their 3-1 victory at home at the end of last year but their 3-2 victory was not enough to advance to the next stage Veronika POLAKOVA claimed both her matches today defeating Annamaria ERDELYI and Mariana SANTA COMBA while Tatiana KUKULKOVA overcame Ines MATOS Zdena BLASKOVA was unable to contribute to the scoreboard suffering defeats against Annamaria ERDELYI and Mariana SANTA COMBA The European Table Tennis Union (ETTU) is the governing body of the sport of table tennis in Europe and is the only authority recognized for this purpose by the International Table Tennis Federation The ETTU deals with all matters relating to table tennis at a European level including the development and promotion of the sport in the territories controlled by its 58 member associations and the organization of continental table tennis competitions French club Saint-Denis 93 TT secured a narrow 3-2 victory over Portugal’s CTM Mirandela in the opening leg of the Europe Cup Women Quarterfinal Universidad de Burgos – RM Terán defeated Hungary’s Budaörsi Sport Club 3-1 with the home side stretched as a team across all five matches Jie LI opened strongly with a dominant win over Maria RUIVO but Annamaria ERDÉLYI quickly leveled the score by defeating Agathe AVEZOU in straight games Hsing Yin LIU restored the lead for Saint-Denis with a 3-1 win over Ines MATOS before ERDÉLYI struck again—this time overcoming Jie LI—to make it 2-2 Agathe AVEZOU delivered a composed performance beating Maria RUIVO in straight games to seal the win for the French side I’m proud to get the last win at 2-2,” said AVEZOU Despite ERDÉLYI’s impressive double victory CTM Mirandela fell just short of the upset they reduced the pressure heading into the return leg on 27th April Saint-Denis 93 TT – CTM Mirandela 3-2 the educative and pastoral communities of the Salesians of Don Bosco in Cabo Verde celebrated the Festival of Youth Holiness with special activities proposed by the Pastoral Ministry and Schools the Salesian Educational Community in Cabo Verde celebrated the Festival of Youth Holiness the event centred on the simultaneous celebration of two Eucharists and sports activities of various disciplines in three different locations the Youth Holiness Festival aimed to look at all models of youth holiness and reflect on their ability to take the initiative with other young people and put their gifts to good use An afternoon of conviviality and willingness shared by all was experienced during the Eucharist The Salesians in Porto celebrated the Feast of Youth Holiness where the pupils of the Don Bosco school took St Dominic Savio as an example joy was the dominant note of the day throughout the various activities the celebration was enhanced by the presence of Fr Tarcízio Morais Superior of the Salesian Province of Portugal and highlighted the simplicity and importance of each person trusting Important figures from the history of the Salesians of the school ANS - “Agenzia iNfo Salesiana” is a on-line almost daily publication the communication agency of the Salesian Congregation enrolled in the Press Register of the Tibunal of Rome as n 153/2007 This site also uses third-party cookies to improve user experience and for statistical purposes By scrolling through this page or by clicking on any of its elements By submitting the above I agree to the privacy policy and terms of use of JTA.org The Alheira is a beautiful thing. A type of Portuguese sausage known for garlic and breadcrumbs, the meat link is so beloved in Portugal that it was voted one of the nation’s seven gastronomic wonders in a 2011 vote. Oh, and it was invented by Jews to save their lives Portugal’s Jews went to great lengths to hide their religion and in a mountain town in northern Portugal called Mirandela the Portuguese would hang preserved pork sausages from their rafters to keep them fed through winter But Mirandela’s secret Jewish community didn’t eat pork and thus: an easy target for anti-Semites looking to turn in their Jewish neighbors So the Jews did what they always do: they turned to food. Mirandela’s Jews invented a new sausage, the Alheira de Mirandela which they made with kosher chicken and bread and promptly hung from their rafters maybe the kosher version of this heroic Jewish sausage will make a comeback JTA has documented Jewish history in real-time for over a century Keep our journalism strong by joining us in supporting independent I accept the Privacy Policy a high proportion of filler is not generally considered a positive In a time when Jews were being persecuted in Rossio Square just metres from where Manteigaria Silva’s cream awning extends today Yet Portugal’s cuisine is more narrative-heavy than most a complex tapestry of invasions and colonisations that slips and slides between continents and religions • The ugly story behind a breakfast meat • The dessert that’s blocked at borders • Why you can’t get ‘Jewish food’ in Israel the most popular and time-honoured ones have stayed with us over many centuries from the period of Moorish rule – also known as a golden era for Jews in Western Europe,” Paolo Scheffer the sophisticated Muslim culture from North Africa that outsiders called the Moors ruled much of Iberia including the hilly city known as Al-Ushbuna A Jewish community had long lived and flourished here Jews and Muslims both left their gastronomic mark on modern-day LisbonFrom marzipan and rosewater pastries to soups citizens of both religions left their gastronomic mark on what is today the city of Lisbon Moorish fish dishes and even Moorish broth which is now a seafood dish called cataplana,” Scheffer observed “But those dishes would have adhered to Judaic and Islamic dietary laws without the popular ingredients added today like shellfish when Christian crusaders first roared through Lisbon the city already had its own culinary culture: Christian elements merged with this established set of flavours as Portuguese navigators spread across the globe chilli and black pepper would leave their mark in turn it’s hard to separate what’s now identified as Christian Portuguese food from the established Arab and Jewish cuisines Ferdinand of Aragon and his warrior queen Isabella of Castile defeated the last Moorish emirate – Granada – and took the Alhambra Palace as their own Ferdinand and Isabella believed that practising Jews might encourage those who had converted to Christianity to go back to their old religion They appointed interrogators to persecute the Jews in their kingdom: their rule of terror would be known as the Spanish Inquisition tens of thousands of Jews who had flourished in Moorish Al-Andalus were thrown out of Spain After overcrowding caused a plague outbreak Christian citizens forced all Jews to live outside the city walls Portugal’s Jews were also forced to convert to Christianity rampaging citizens and sailors killed thousands of converted Jews in a citywide pogrom the Inquisition formally arrived in Portugal and soon both practising Jews and Jews who had converted to Christianity were among the unfortunates parading in penance or burnt at the pyre in Rossio Square Jews went to huge lengths to conceal their faithDisguising themselves as Christian converts Portugal’s secret Jews went to huge lengths to conceal their religion – from writing Hebrew prayers in Catholic prayer books to combining Jewish words with Catholic rituals (One community in Belmonte kept its faith alive in secret for more than 400 years.) In the rugged mountains of northern Portugal’s Trás-os-Montes one of these hidden communities created Portugal’s best-known alheira sausage: Alheira de Mirandela In Trás-os-Montes every home preserved pork sausages to see the family through the winter hanging them from the rafters in meaty coils Jews – who did not eat pork – were conspicuous for their missing sausages “They were seeking refuge from the Inquisition,” Scheffer explained “So the town of Mirandela developed a bread sausage that could fool informers and local zealots who denounced them to the Inquisition for not eating pork.” the Alheira de Mirandela seems very like kishke meal and flavourings that’s often served in the slow-cooked Jewish Sabbath bean stew known as cholent The Jews of Trás-os-Montes traditionally made theirs with bread and chicken although a present-day Alheira de Mirandela is no longer kosher and can include everything from pork to game the alheira has travelled far beyond the mountains It’s ubiquitous in supermarkets and appears alongside steak and eggs in workers’ cafes or neighbourhood diners At Zé dos Cornos a white-tiled little place below Lisbon’s Castelo de São Jorge I watched local workers chow down from huge rectangular plates chunks of juicy game mingled with chunks of sour the hillside district where they lived is known as Mouraria (Moorsville) But it was not until the early 19th Century that Jews began to return and there were no more than 1,000 Jews in Lisbon the neutral city again became a refuge for Europe’s Jews Portuguese diplomat Aristides de Sousa Mendes issued travel documents to thousands of Jews: more than 10,000 Jews would set sail from Lisbon to safety across the Atlantic although towns and cities across Portugal are beginning to rediscover their Jewish history the alheira is more a part of mainstream Portuguese cooking than a symbol of the people who created it Like the Portuguese word for ‘Saturday’ – 'Sábado' for the Jewish Sabbath – and the brilliant Arab-influenced tiles that illuminate Lisbon's teetering streets the sausage is an indicator of a past as cosmopolitan as it is complex Culinary Roots is a series from BBC Travel connecting to the rare and local foods woven into a place’s heritage Join over three million BBC Travel fans by liking us on Facebook, or follow us on Twitter and Instagram If you liked this story, sign up for the weekly bbc.com features newsletter called "If You Only Read 6 Things This Week". 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All News FIDE News Chess News Top Top Federations Main Page / Search Tournaments Titles Transfers Calculators Download FIDE Circuit Women's Events '24-'25 Open Cycle 2025-2026 Women’s Cycle 2025-2026 Women’s Cycle 2023-2025 All Tournaments Main Events About FIDE Handbook Documents Financial Reports Officials Commissions & Committees Federations Affiliated Organizations Affiliated Members Honourable Dignitaries Chart GM Carlos Daniel Albornoz Cabrera from Cuba emerged as the winner of the III Open Xadrez Terras Trás-os-Montes 2022 one of the strongest open tournaments held in Portugal in recent years took place in Mirandela from August 17-23 and brought together 131 participants from 26 federations confirmed his status as the main favourite and took the first prize scoring an impressive 8/9 the winner was awarded a chess set signed by Judit Polgar IM Arthur Pijpers (Netherlands) and IM Jakub Kosakowski (Poland) finished a full point behind the champion and tied for second place The Buchholz tiebreak favoured Leniart and Pijpers taking second and third positions on the podium Several young players came to the final round seeking different norms but only FM Guy Levin from Israel succeeded and completed an IM norm Check out the complete results here © 2025 FIDE International Chess Federation stored in a retrieval system or transmitted in any way or by any means (including photocopying recording or storing it in any medium by electronic means) without the written permission of FIDE International Chess Federation Portugal’s CTM Mirandela triumphed over Czech club HB Ostrov in the opening leg of the Stage three of the Europe Cup Women Annamaria ERDELYI delivered two wins against Veronika POLAKOVA and Zdena BLASKOVA Mariana SANTA COMBA added the third point with a hard-fought five-game victory over Jana VASENDOVA BLASKOVA secured the sole point for HB Ostrov by defeating Ines MATOS We seized the opportunity to secure an advantage before the return leg in Czechia,” said ERDELYI It’s great that Mariana managed to beat VASENDOVA The return leg is scheduled for February 16th jovem jogador com raízes familiares em Mirandela assinou contrato com o Vitória de Guimarães para as próximas quatro épocas confirmou o clube no seu canal de comunicação O jogador de 21 anos vai ser orientado pelo também mirandelense passou pelo Padroense e posteriormente pelo Desportivo de Chaves O jovem mirandelense estreou-se oficialmente pela seleção nacional de Sub/17 ao marcar presença nos três jogos da 43.ª edição do Torneio Internacional do Algarve Mirandela went the full distance but succeeded in France In the opening leg of the Europe Cup quarterfinal Clube Ténis de Mesa de Mirandela secured an away win against JOUE LES TOURS TT After a full five matches they overcame French club Li HE handled the pressure exceptionally well defeating both Jingyi ZHOU and Annamaria ERDELYI ZHOU and ERDELYI on the other hand showed no weaknesses in their duel against Audrey ZARIF Equally important Ines MATOS won a very close duel against Nolwenn FORT JOUE LES TOURS TT – Clube Ténis de Mesa de Mirandela 2-3 Li HE – Jingyi ZHOU 3-2 (11-6 9-11 11-5 9-11 6-1) Audrey ZARIF – Annamaria ERDELYI 0-3 (7-11 9-11 4-11) Nolwenn FORT – Ines MATOS 2-3 (12-14 11-9 11-7 7-11 1-6) Li HE – Annamaria ERDELYI 3-0 (12-10 11-7 11-9) Audrey ZARIF – Jingyi ZHOU 1-3 (7-11 11-8 7-11 9-11) The European Champions League Women (ECLW) organised by the European Table Tennis Union (ETTU) is the most important international club competition which replaced European Club Cup of Champions (ECCC) Hungarian club Statisztika Budapest still holds remarkable record They were on the top 11 consecutive seasons (1976-86) Romanian Vointa Arad was the winner of the inaugural 1963/64 season The competition came into a new era in 2005/06 when the European Champions League Women started In an all-French Champions League Women’s semifinal Metz TT overcame Saint-Quentin TT in the second.. Reigning champions KTS ENEA SIARKOPOL Tarnobrzeg were forced to surrender their title even before reaching.. Reigning champions KTS ENEA SIARKOPOL Tarnobrzeg and Saint-Quentin TT emerged victorious in the opening leg.. The Champions League Women semifinal lineup is now complete In their debut appearance in the Europe Cup CSM Constanta reached the semifinals of the Women’s Cup Joining the Romanian club Portugal’s Clube Ténis de Mesa de Mirandela also secured a place in the penultimate stage CSM Constanta defeated GRAND-QUEVILLY ALCL 3-1 in the away match in France after prevailing 3-2 at home Reaching the semifinal in our first year of the ETTU Cup season is a significant achievement I am thrilled to have won two matches for my team; last time my sister contributed with two victories as well Additionally Bianca did her best showing her high level and securing one match win Given the high stakes we felt the pressure but we overcame it together Supporting each other we remained focused from the beginning until the end Despite facing really strong opponents we were fully aware of their capabilities In the end the collective power of our team prevailed ” said Elena ZAHARIA Clube Ténis de Mesa de Mirandela also prevailed in both legs against JOUE LES TOURS TT In the opening leg it was a long-distance duel while tonight Annamaria ERDELYI Jingyi ZHOU and Ines MATOS showed no mercy “After we won the opening leg we knew that we had a chance That opportunity became even better when we saw the lineup of the opposing team without Audrey ZARIF Of course it gave us a certain dose of confidence but winning 3-0 was never on the table I opened the match very well and the crucial moment was the victory of our player from Singapore Jingyi ZHOU over the Chinese player Li HE After that the very good performance of Ines MATOS sealed the match ” said Annamaria ERDELYI In the semis Clube Ténis de Mesa de Mirandela awaits the winner of the match HB Ostrov z.s GRAND-QUEVILLY ALCL – CSM Constanta 1-3 Samson LI – Alina MUSAT (ZAHARIA) 3-0 (11-6 11-4 11-9) Jiaduo WU – Elena ZAHARIA 1-3 (7-11 7-11 11-6 11-13) Anais SALPIN – Bianca MEI ROSU 2-3 (8-11 8-11 11-7 11-2 5-6) Samson LI – Elena ZAHARIA 1-3 (5-11 11-8 6-11 7-11) Clube Ténis de Mesa de Mirandela – JOUE LES TOURS TT 3-0 Annamaria ERDELYI – Clemence CHEVALLIER 3-0 (11-6 11-8 11-2) Jingyi ZHOU – Li HE 3-1 (6-11 11-7 11-6 11-9) Ines MATOS – Nolwenn FORT 3-1 (13-11 17-19 12-10 11-6) After an intense final series in the Portugal League play-off Sporting CP emerged victorious over CTM Mirandela clinching their 15th title in the club’s history The defending champions handed over the trophy to the new winners In the opening match Sporting CP defeated Mirandela by a close score of 3-2 However Mirandela quickly leveled the score setting the stage for a decisive clash Once again Sporting CP found themselves pushed to the limit but ultimately came out on top The pivotal moment in the quest for the title came during the opening duel In the fifth and final game of the fifth match Galia DVORAK of Sporting CP triumphed over Annamaria ERDELYI of Mirandela sealing the victory with a score of 15-13 “I believe that the moment that truly made the difference was the first match when we won 3-2 and sealed it with a 15-13 victory in the deciding game The level of both teams was incredibly even and winning two matches in a row seemed unlikely for either side Despite our loss in the second encounter we remained positive and knew that if we capitalized on our chances we could secure the title… and we did ” expressed Galia DVORAK She further added “I am incredibly satisfied because I wasn’t sure if I could still perform at this level but I proved that I could We have faced each other many times in the past so we both understand the intensity of this battle.” Sporting CP’s triumph in securing their 15th trophy highlights their dominance in Portuguese table tennis The team’s resilience and determination throughout the final series ultimately paid off solidifying their place in the club’s rich history Annamaria ERDELYI Matilde PINTO – Anna HURSEY Galia DVORAK 3-2 (9-11 12-10 14-16 11-2 11-6) Inês MATOS – Anna HURSEY 0-3 (9-11m 6-11 10-12) Annamaria ERDELYI – Galia DVORAK 0-3 (9-11 7-11 8-11) Matilde PINTO – Patrícia SANTOS 3-1 (11-9 11-5 8-11 11-7) Inês MATOS – Galia DVORAK 0-3 (10-12 10-12 5-11) Annamaria ERDELYI Matilde Pinto – Anna HURSEY Galia DVORAK 3-1 (11-5 11-9 7-11 11-9) Inês MATOS – Galia DVORAK 1-3 (11-4 5-11 12-14 10-12) Annamaria ERDELYI – Patrícia SANTOS 3-0 (11-3 11-3 11-5) Matilde PINTO – Anna HURSEY 0-3 (7-11 8-11 8-11) Inês MATOS – Patrícia SANTOS 3-2 (5-11 11-7 11-8 8-11 11-8) Patrícia SANTOS Anna HURSEY – Inês MATOS Matilde PINTO 3-0 (11-5 12-10 11-6) Galia DVORAK – Inês MATOS 1-3 (8-11 8-11 12-10 11-13) Patrícia SANTOS – Annamaria ERDELYI 1-3 (18-16 9-11 12-14 9-11) Anna HURSEY – Matilde PINTO 3-0 (11-6 11-8 11-5) Galia DVORAK – Annamaria ERDELYI 3-2 (11-5 14-12 6-11 9-11 15-13) The Europe Trophy Grand Final in Mirandela concluded with the won of the French TT Joué les Tours On the final hurdle they succeeded against Greek club ASEA Sarises Florinas In the ultimate stage Audrey ZARIF LI He and Nolwenn FORT overcame Bernadett BALINT LIU Judith and Despoina AMPA in straight matches “It is first time for the club that we won the European Trophy and we are very happy I hope we will be equally successful next season in t he Europe Cup ” said coach Hugo BERGER Audrey ZARIF added: “It was a long week-end with a lot of matches It was really important for the club to win this title so we gave our best Some matches was more difficult than other we won all the matches 3/0 so that nice.” In the semi final Entente Saint Pierraise TT faced first defeat at the tournament Greece’s ASEA Sarises Florinas caused the big upset and won the difficult five matches duel to secure the ultimate stage They were 2:0 up before they withstood spirited comeback from Entente Saint Pierraise TT to win the place in the final in the decisive match “We had very tough match to reach the final but we know how difficult will be against next adversary We already lost to Joue Les Tours in the group phase However we will stay focused and we will fight hard ” said coach Mark PINK before the final Joue Les Tours was the first team proceeding to the final They overcame Croatia’s STK Aquaestil Duga Resa “We are really happy that we won this match We will give our best to win this Trophy ” said Audrey ZARIF On the opening day Joue Les Tours overcame Portugal’s Uniao Sebastianense F.C Norway’s B-72 and Greek ASEA Sarises Florinas ASEA Sarises Florinas – TT Joué les Tours 0:3 Bernadett BALINT – Audrey ZARIF 2:3 (11:7 8:11 4:11 11:9 1:6) LIU Judith – LI He 1:3 (6:11 9:11 13:11 4:11) Despoina AMPA – Nolwenn FORT 0:3 (9:11 7:11 4:11) Entente Saint Pierraise TT – ASEA Sarises Florinas 2:3 Stephanie LOEUILLETTE – LIU Judith 2:3 (11:7 11:4 4:11 7:11 4:6) Lucie GAUTHIER – Bernadett BALINT 0:3 (9:11 8:11 5:11) Jeanne ROBBES – Despoina AMPA 3:0 (11:8 11:5 11:6) Stephanie LOEUILLETTE – Bernadett BALINT 3:0 (11:6 11:9 11:6) Lucie GAUTHIER – LIU Judith 1:3 (11:9 7:11 8:11 6:11) TT Joué les Tours – STK Aquaestil Duga Resa 3:0 Audrey ZARIF – Leeloo HAN VUKELJA 3:0 (12:10 11:5 11:5) LI He – Xue HAN VUKELJA 3:2 (11:5 11:2 9:11 5:11 6:4) Nolwenn FORT – Sofija SHEREDEHA 3:0 (11:3 11:6 13:11) CTM MIRANDELA – Uniao Sebastianense F.C Ines MATOS – Cecilia AKPAN 3:2 (11:9 11:5 3:11 5:11 6:2) Matilde PINTO – Raquel MARTINS 3:0 (11:7 11:2 11:4) Mariana SANTA COMBA – Leila OLIVEIRA 0:3 (7:11 9:11 12:14) Ines MATOS – Raquel MARTINS 0:3 (9:11 8:11 9:11) Matilde PINTO – Cecilia AKPAN 2:3 (11:9 9:11 11:7 3:11 0:6) Tereza PYTLIKOVA – Maria HORGEN 3:0 (11:2 11:5 11:6) Aneta SIRUCKOVA – Vivian HUYNH 3:0 (11:4 11:2 11:5) (ANS - Mogofores) - The Extraordinary Visitation that the General Councilor for Social Communication continues to the Salesian Province "Saint Anthony" of Portugal The last stops were at the Salesians of Mogofores and Mirandela Fr Mendes visited Mogofores in the last days from 22 to 24 April and punctually at 8 am on the first of the 3 days he was received by the Director and by the other Salesians of the community Then he offered the thought of the Salesian “Good morning” to the school's students and explained all the meaning of his visit: to be with his brothers to praise God for what is being done there to offer useful suggestions and indications He also brought the greeting of the Rector Major and his paternal "concern" to help the Congregation grow in the beauty of the Salesian charism in the apostolic holiness of every religious and in pastoral service to the poorest and most marginalized young people he spent a lot of time listening: to fully understand the characteristics of the work of Mogofores and the environment in which it is inserted; before offering the best ways to follow to achieve goals and projects He conversed with the entire Educational Community of the School with the members of the Salesian Family and the Parish Pastoral Council and often interacted with the students of the institute the General Councilor for Social Communication reached Mirandela where he carried out the previously planned program involving the people who animate the main sectors of the Salesian presence - the Salesian community the “St John Bosco” parish with its pastoral project He also met the diocesan bishop and dedicated himself to getting to know the surrounding situation Fr Mendes had various meetings with the Salesian community and met the community council the members of the technical team and had a meeting with all the collaborators who make up the educational and support teams He then gathered with the leaders of the parish animation teams whom he congratulated on their ability to engage in the process of education and evangelization typical of a Salesian parish He expressed his appreciation of the geographical position of the Salesian house from the point of view of landscape and ecology and noted the good relationship that exists with the various entities and institutions of the city And during the visit to the diocesan bishop the work carried out by various generations of Salesians who have passed through that city the Salesian imprint of the parish of St John Bosco and the need to strengthen the educational-pastoral intervention in the Polytechnic Institute of Bragança members of the community of the Salesian parish of "St John Bosco" stood in solidarity with the people of Ukraine by raising their prayers for peace in that country would inspire those with decision-making abilities The prayer was led by the parish priest Fr João de Brito and enriched by various interventions a video was projected with some images of the events the choirs of young people and adults sang some songs of peace to Our Lady representatives of each catechetical group the prayer for peace in Ukraine was recited which ended with a song entitled "Miraculous Queen of Heaven" This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page pastoral teams and Salesian Social Services join together at this time of year to promote traditional Christmas Campaigns in the various communities the solidarity campaign will help two associations this Christmas The value of admission tickets to the three schools' Christmas parties will be donated to the "Ajuda de Berço" Association will be given to "Turma Solidária," a social solidarity association in Alcabideche "SolSal - Family Support Service" is promoting the "Hands that Multiply" food collection campaign among the educational community invited everyone to live the true spirit of Christmas and to convert the usual exchange of gifts into help for Christmas offerings to be delivered to more than 200 families accompanied by the "SolSal" service and the parish "Solidarity Bazaar." A solidarity campaign has also been underway at the Salesian School in Funchal since the beginning of December to provide a little more comfort to the neediest families in and around the school Cycle 1 students have been asked to contribute basic necessities for personal hygiene Cycle 2 and 3 students brought food items from a list requested by the school and staff of the Lisbon Salesians were asked to collaborate with donations for the Christmas Campaign to benefit needy families The main needs were studied and a list was compiled and distributed to the various classes and groups; until December 16 students were able to bring hygiene products Particular and well thought out was the campaign initiated in Manique where the 57 classes of the Salesian institute were invited to make a Christmas basket based on the characteristics of each family (number of items which lasted from November 21 to December 16 the Christmas collection campaign will benefit the Children and Youth Home and the needy in the eight parishes entrusted to the Salesians the Salesians in Mirandela also support students in some of the city's institutions through the distribution of clothing The National Shrine of Our Lady Help of Christians in Mogofores is collecting food and gifts with the help of the parish's social store and youth groups that will participate in WYD Lisbon 2023 The distribution of gifts during the Christmas celebration is a Shrine tradition And parishioners can contribute by delivering their offerings during the Eucharists on the four Sundays of Advent or weekdays directly to the Shrine's Pastoral Office the Association of Parents and Guardians of Pupils of the Porto Salesians has also launched a food collection drive The baskets will benefit needy families in the various work areas of the school (ANS - Dili) - Fr João Pires de Deus the oldest Salesian of the East Timor Vice Province after 61 years of missionary life spent in East Timor and 53 as a Salesian Fr João de Deus was born in Mirandela in 1963 he founded the "Fatumaka Technical School of Agriculture" and supported the development of Catholic missions in Baucau the Portuguese government ordered the immediate evacuation of all Catholic missionaries from East Timor but Fr João de Deus decided to stay in the country despite the risks and dangers he was running In that year he was captured by the Indonesians and was released only a few weeks later After the dismantling of the bases in 1978 the Catholic Church was the only institution that remained with the population during the Indonesian occupation the Portuguese missionaries built orphanages in Baguia Fr João remained there and continued to support the local population In 2012 he was awarded the Medal of the Order "Martinho Lopes" an order created by the State to recognize the contribution of the Catholic Church in the national liberation of East Timor and to offer thanks for the support and closeness to the local population President of the Democratic Republic of East Timor personally offered his last farewell to Fr João de Deus going to the Chapel of Don Bosco in Comoro the President expressed the greatest gratitude for the contribution that Fr João had made for national independence President Guterres then signed a presidential decree where he once again expresses his thanks for what Fr João de Deus did in the most difficult years for the country The East Timorese Head of State then brought his condolences to the Salesian Family for this great loss Registration has been successfully completed Make a new account if you don't have one yet Puedes ver la versión Española de BeSoccer.com You can see the English version of BeSoccer.com Vous pouvez voir la version French de BeSoccer.com Puoi vedere la versione Italian su BeSoccer.com Você pode ver a versão Brasileira de BeSoccer.com.