We deliver! Get curated industry news straight to your inbox. Subscribe to Adweek newsletters HBO’s The Last of Us and Corona are raising a glass to the new season and a new partnership Heading into the Last of Us Season 2’s premiere in April Discovery Ad Sales and Corona teamed up for the “La Playa Awaits” campaign which celebrates Season 2 of the zombie series as well as the alcohol brand’s 100th anniversary the idea is to have a beach mindset no matter where you are—even if you’re in a post-apocalyptic world overrun by zombies infected by parasitic mushrooms 5 Immersive Brand Collabs for The Last of Us Season 2 The campaign activated around key series events including providing Corona Extra and non-alcoholic options for guests at the March 24 premiere at the TCL Chinese Theater in Los Angeles as well as the sponsorship of an influencer pre-screening of the first episode at the Domino Sugar Factory in Brooklyn Corona also provided the title sponsorship and supporting media in the first and second seasons of The Last of Us on Max Corona also debuted two 30-second custom ads featuring The Last of Us star Gabriel Luna The ads show Luna making the most of the post-apocalyptic situation after discovering an ice-cold Corona beer The spots aired on Max’s ad-supported plan ahead of The Last of Us episodes and across social media via the CoronaUSA and Max accounts “I’m proud to partner with Corona to help celebrate ‘La Playa Awaits’ through a world that parallels the one I exist in as Tommy on the HBO Original series The Last of Us,” Luna said in a statement to ADWEEK “‘La Playa Awaits’ represents the beach mindset inviting people to make the most of the present moment which is something I strive to do every day if only Tommy could stumble upon a cold Corona.” Corona launched a retail activation and sweepstakes with co-branded assets in select retail environments Fans of the show and the beer brand have the chance to enter a giveaway to win exclusive The Last of Us merchandise including a special edition Taylor Guitar 314 replica like the one that appeared in the series “Partnering with the HBO Original series The Last of Us is another way we seek to immerse into our consumers’ passions,” Rob Nelson ‘La Playa Awaits,’ reminds us all of the power of being more present in the moment which is embodied in this partnership—whether through the characters in the construct of the show itself or as viewers collectively coming together and tuning into one of the most anticipated seasons in television history.” In addition to Corona, The Last of Us also recently rolled out collaborations with Four Sigmatic Saleah Blancaflor is a TV reporter at ADWEEK Adweek is the leading source of news and insight serving the brand marketing ecosystem May 5, 2025 3:02 PM EDTAmericans love their beer but the best-selling beer in the United States is imported from Mexico Modelo Especial overtook Bud Light to become the top-selling beer brand in America is still flying off the shelves in the U.S According to Darren Rovell of cllct media and Kickstand Cocktails, via BevNet Modelo has been the most purchased beer in America over the last year Coors ($3.13 Billion) and Corona ($3.12 billion) round out the top five Both Corona and Modelo Especial are made by the Grupo Modelo brewery south of the border The best cheap beers in America range from Budweiser to Miller Lite to Corona to Modelo feeling fresher and more authentic.” Bernot added that the Mexican imports are "just cooler brands," and their rise is indicative of a surge in Latin American culture in America in other areas “We see it in music as well,” Bernot said “Look at Bad Bunny and Nathy Peluso It matters that these beers have cultural cache at a time when Hispanic cultural exports of all kinds—food music and art—are enjoying a moment in American mainstream culture.” As for the American brands, Miller can at least take solace in being named "America's favorite beer" in a recent study by Coffeeness, a German-based website.  Miller was the preferred brew in 30 of 50 states, finishing well ahead of Coors, which placed second as the favored beer in eight states. By Andrew Holleran is a trending news writer on Men's Journal He's covered sports and pop culture for more than a decade How to watch California high school boys lacrosse: Loyola vs May 6Data SkriveWe have an exciting high school game in Los Angeles with Loyola High School hosting Corona Del Mar High School How to watch Corona Del Mar vs. Loyola boys lacrosseCorona Del Mar and Loyola will hit the field on Tuesday, May 6, at 5 p.m. PT. Don't miss out on any of the action with NFHS Network The NFHS Network gives you access to live high school sports around the country Follow your favorite team and never miss a game Want to track your alma mater, local team or family member's school throughout the playoffs? NFHS Network has high school lacrosse streaming live plus on-demand replays and highlights of all the action from thousands of schools across America Watch Loyola vs. Corona Del Mar on NFHS Network! All NFHS Network events are available to watch online at and through the NFHS Network Mobile Apps for iOS and Android and TV Apps for ROKU The team analyzed radio-frequency data from the Parker Solar Probe (PSP) but did not find any spectral distortions that would have been dark-photon evidence The researchers thus place the strongest limits yet for dark photons with masses around 10−9eV/c2 The dark photon is an appealing dark-matter candidate because it can arise naturally within certain string-inspired theories these models do not fix the mass of the dark photon searches have spanned many orders of magnitude in mass from 10−18 to 106 eV/c2 The ultralight dark photon (less than about 1 eV/c2) stands out as it offers distinctive cosmological effects and novel experimental signatures Remarkably, we may not be completely blind to the dark photon, as it could interact with normal particles through a phenomenon known as kinetic mixing [4] This feeble coupling effectively allows dark photons to “oscillate” into ordinary photons and vice versa much like neutrinos oscillate between different flavors This oscillation can be enhanced in certain environments One such environment is the solar corona—a superheated ionized gas extending millions of kilometers into space around the Sun The electrons in this plasma interact with normal photons causing them to behave as if they had a mass The value of this effective mass depends on the corona’s electron density which decreases with distance from the Sun If a dark photon passes through the solar corona and its mass is equal to the effective mass in a particular region of the plasma then the dark-to-normal conversion will be dramatically enhanced through a resonant interaction The resulting photon is highly monochromatic with an energy equal to the dark photon’s mass Some of these converted photons will be absorbed or scattered in the plasma, but a fraction of them will escape, leading to an observable “bump” in the spectrum of the corona. Researchers have previously looked for this dark-photon signature using ground-based radio telescopes [5] These observations have been limited to photon frequencies above 10 MHz corresponding to dark-photon masses above 10−6 eV/c2 The reason for this limited range is that radio frequencies below approximately 10 MHz are reflected back into space by Earth’s ionosphere ground-based radio telescopes suffer from an attenuated signal because of the vast distance between the Sun and Earth To address these limitations, An and his colleagues have used the PSP as an in situ dark-matter detector. The PSP is a NASA mission designed to study the solar corona by flying closer to the Sun than any previous spacecraft (see Special Feature: To Touch the Sun) the probe reached its closest perihelion distance of approximately 10 solar radii in July 2022 but its highly elliptical orbit has allowed it to study up close the corona’s plasma over a wide range of radii—and thus over a wide range of electron densities The PSP’s two radio receivers are capable of measuring frequencies ranging from about 20 kHz to 20 MHz Using these radio data and the orbital path of the probe An and colleagues could look for spectral bumps corresponding to dark-photon masses between 3 × 10−10 and 8 × 10−8 eV/c2 For smaller dark-photon masses (and equivalently smaller frequencies) the researchers also considered data from the Solar Terrestrial Relations Observatory (STEREO) which maintains a fixed orbital distance of 1 astronomical unit from the Sun The search for dark-photon dark matter remains vigorous having made significant progress over the past decade The work of An and colleagues carves out a novel pathway limiting lightweight dark photons to have either an extremely feeble kinetic mixing parameter or a very low mass (below 10−15 eV/c2) where the parameter space remains relatively open An interesting way to improve the sensitivity to ultralight dark photons involves resonant LC circuits—sometimes called dark-matter radios These experiments aim to detect the dark photon’s tiny oscillating electric fields by tuning the circuit to the particle’s Compton frequency allowing dark photons to induce a faint but measurable current Several such experiments are on the horizon Paola Arias is a particle physicist based in Santiago She earned her PhD from the University of Santiago and has held two postdoctoral research positions: one at the German Electron Synchrotron (DESY) in Germany as a Humboldt fellow; and another at the Pontifical Catholic University of Chile Her research focuses on extensions of the standard model with a particular interest in WISPy particles She is deeply fascinated by the interplay between particle physics and cosmology especially in how it can help us uncover the properties of dark matter Phys. Rev. Lett. 134, 171001 (2025) The planned MUonE experiment could—in addition to studying the muon’s magnetic moment—search for dark matter particles. Read More » Measurements of millions of galaxies suggest that dark energy changes over time and is more complicated than previously thought. Read More » The Euclid satellite released its first trove of galaxy data based on seven days of deep-field observations in three sky areas. Read More » Researchers have shown that they can distribute quantum keys under realistic conditions using commercial lasers Student enrollment and guaranteed financial support are expected to fall amid anticipation of federal budget cuts Two independent teams have searched for axions using x-ray observations of entire galaxies setting some of the strictest constraints to date on the properties of these dark matter candidates More Recent Articles » Sign up to receive weekly email alerts from Physics Magazine Use of the American Physical Society websites and journals implies that the user has read and agrees to our Terms and Conditions and any applicable Subscription Agreement. , opens new tab. The levies went into effect on April 4.The move would potentially drive up prices of cocktails champagne and foreign beers and wipe out jobs in the spirits industry according to drinks industry bodies and analysts.Late on Wednesday Trump said he would temporarily lower the hefty duties he had just imposed on dozens of countries while further ramping up pressure on China.Constellation Brands also projected annual enterprise organic net sales between a 2% decline and 1% growth while forecasting wine and spirits income to slump up to 100%.Beer sales made up nearly 82% of total fiscal 2024 revenue for Constellation Brands while wine was about 16%.It posted fourth-quarter net sales of $2.16 billion beating estimates of $2.13 billion.Reporting by Savyata Mishra in Bengaluru; Editing by Maju Samuel Our Standards: The Thomson Reuters Trust Principles., opens new tab , opens new tab Browse an unrivalled portfolio of real-time and historical market data and insights from worldwide sources and experts. , opens new tabScreen for heightened risk individual and entities globally to help uncover hidden risks in business relationships and human networks. © 2025 Reuters. All rights reserved — In the first eight years that Bob Mauger taught his auto body course at Corona High maybe three balls were hit into his classroom area located well beyond the baseball team’s left-field fence The peace and quiet to which he’d become accustomed ended on the first day of practice last fall He stormed up to the field in a huff; his sanctuary was suddenly the site of what felt like target practice “The balls were just raining down on my classroom,” Mauger recalled Head baseball coach Andy Wise had started to run toward Mauger’s classroom This has never happened in all the years I’ve been here.’” Mauger was certain this was the result of some thoughtless drill perhaps one that required hitting from the outfield grass the coaches were taking swings — it had to be them because high schoolers couldn’t hit the ball this far Corona baseball was built from scratch into one of the nation’s most formidable powerhouses The best team in the most populous state resides at a school that never could have imagined this level of success it has three players projected to be first-round MLB Draft picks: pitcher Seth Hernandez shortstop Billy Carlson and infielder Brady Ebel it would be the first time in the draft’s history that one high school produced three first-rounders — with an outside chance at a fourth amid a season that might be once in a lifetime And there is perhaps no better measure of its prowess than the damage it’s done to one teacher’s classroom potential new players or fans who have flooded this school it’s Mauger’s body shop — and the 40-foot fence the school will build to protect the rest of the classrooms — that serves as the most tangible measurement of growth for this program and the players in it tell everybody,’” Wise said he told Mauger who initially wanted the principal to step in Corona faced something it will seldom experience this year: a deficit was a celebration by the opposition akin to winning a championship His team wasn’t even using its full complement of starters the Panthers are 6-0 and have outscored opponents 35-1 They will be every opponent’s biggest game “I told the team they kind of remind me of us Los Angeles Dodgers third-base coach Dino Ebel who often throws BP or fungoes with the team “We’ve got a target on our backs everywhere we go The Ebels are a great example of the benefits of building Dino’s sons transferred from Etiwanda High to play for Corona They liked the way Wise coached fundamental baseball He came with his parents to watch Corona’s games before eventually enrolling who transferred in this season at the suggestion of Hernandez and Carlson It’s so anomalous to what’s possible that there’s no way it can be sustained at this level It’s a product of building a culture so strong that the best baseball players from all over will do whatever it takes to get a roster spot “I was a little freshman kid just trying to make my way who has been with the program his entire high school career “To see the growth of Corona and the program is super special.” Carlson is the only player among Corona’s group of stars who has known only one school He developed into a prospect and is rated No Wise made a point to stand behind first base as his shortstop took grounders highlighting how assured he was by his shortstop’s accuracy He’s committed to Tennessee and says he’s confident he’ll stick as an infielder in the professional ranks Hernandez plans to pitch at the next level Scouts line the bleachers anytime he’s on the mound 5 overall draft prospect and is widely considered the best high school pitcher available “I think it’s definitely a lot of pressure but only if you think about it too much,” Hernandez said turn into a man — some crazy things can happen.” Bingaman is the player who everyone comes to see Instead, he’s a relative afterthought in the national conversation surrounding this team because he will have to play his way into the first round this year, whereas his more publicized teammates will have to play their way out of it. The Athletic’s Keith Law had Carlson and Hernandez within his top 30 draft prospects “There’s no way that you can say anybody in the lineup is better than Ethan Bingaman,” Wise said Is that a slap in the face to a Brady or a Billy And it’s one Bingaman — a two-way player with speed “I could see myself in the first round,” Bingaman said “I’ve just got to keep proving people wrong.” San Francisco Giants starting pitcher Tristan Beck will regularly log in to online streams to watch Corona The seeds of the success date back to veteran MLB reliever Joe Kelly who graduated from the school nearly 20 years ago He also played a critical role in bringing the Ebels on board this year whom Wise credits as being the catalyst for the most recent iteration of Corona High baseball In the aftermath of his tenure at the school the team has gotten better and more players have gone to top colleges “It’s one of the best high school teams of all time Beck and Kelly are the only big leaguers to come from Corona since 2001 and the school has produced only five in its history That’s because Corona is not the biggest school News & World Report ranks it as the sixth-best school in the district and 692nd in the state of California “This is the lowest socioeconomic school in the district,” Wise said They don’t come from the prototypical SoCal powerhouse mold — think Harvard Westlake Junipero Serra and Huntington Beach — but that’s exactly where this is headed pitcher Ethan Schiefelbein was selected by the Detroit Tigers with the No Corona hopes to have multiple players drafted Athletic director Jeff Stevens said the winning has brought a different set of challenges — namely a lot of people from all over hoping to transfer into the program He has to explain all the various requirements to parents and stress that playing time won’t be guaranteed “When we did what we did last year,” Stevens said referring to Corona’s California Interscholastic Federation Division I championship “I was getting emails from families in Mexico saying ‘We want to transfer to your school.’ I had a family reach out to me from Puerto Rico.” It’s remarkable for a school that has no real advantages and its current building was constructed in 1907 It does not have the resources or amenities “It makes me feel good that people have the decision of where they want to go,” Wise said “I ain’t asking or begging or telling nobody to come here.” Wise’s attention is always getting pulled in different directions all of whom want to be at games or practices Wise is the brash but approachable mastermind behind this team’s success And after losing just three games last year he believes his program can go undefeated this season Baseball is a weird game in that failure is inevitable at some point A bad outing on the mound or a few bats go cold for a day and an upset loss could be the result That conventional wisdom might not apply to this team “We should be favored in every game,” Wise said “If we’re going to run out a pitcher — the other team might have a great pitcher I feel like we could go undefeated if we play our game the right way,” Brady Ebel said “Win every tournament we’re in and hopefully become CIF champions back-to-back years.” The players say they haven’t allowed themselves to think much about draft day — where they’ll be or who they’ll spend it with all this attention was brought on so quickly It wasn’t long ago that Carlson was committed to Vanderbilt so he switched that commitment to Tennessee a choice rooted solely in his baseball future It’s with that understanding that all these players and coaches operate Not even their names being called on July 13 they’re focused on what’s in front of them: a high school baseball season the likes of which we have never seen before and might never see again “It’s going to be history,” Hernandez said of this season and the upcoming draft “I think that’s pretty cool to leave a legacy behind at this place.” (Top photo of Billy Carlson: Gia Cunningham / Courtesy of Corona High School) Sam Blum is a staff writer for The Athletic covering the Los Angeles Angels and Major League Baseball. Before joining The Athletic, he was a sports reporter for the Dallas Morning News. Previously, he covered Auburn for AL.com and the University of Virginia for The Daily Progress in Charlottesville. Corona celebrates its 100-year anniversary, a remarkable milestone for the iconic brand that has been synonymous with the beach and enjoyed by consumers worldwide for the past century. Since 1925, Corona has cultivated a deep association with the beach; fully embodying a lifestyle connected to nature and relaxation. In honour of the occasion, Corona invites everyone to live their 'beach side' – a.k.a. their best side – at top-tier beach locations across the globe. The Corona 100 platform includes a film highlighting 100 years of beach culture, a definitive list of the top 100 beaches in the world to visit, and a signed multi-year sponsorship of a renowned concert at Copacabana Beach in Rio de Janeiro — all offering people across the globe opportunities to connect with their beach side. To honour a century of beach side living, the brand has created its Corona Beach 100 list: a curated guide of the world’s most iconic beaches that best represent Corona’s 'This is Living' expression. From hidden coves to legendary coastlines to remote paradises, these beaches aren’t just destinations; they’re places where people have travelled for centuries to disconnect from the real world and reconnect with nature. The guide includes sandy beaches from famed hotspots within South Africa, Mexico, and Brazil, as well as showcases some lesser travelled shorelines, such as Chile’s Punta de Lobos, Iceland’s Stokksnes Beach, and Canada’s Cox Bay Beach, among many more. Additionally, Corona is offering the chance to visit the iconic beaches featured on the Beach 100 list. Anyone of legal drinking age can enter to win a chance to tap into their beach side at one of the 100 crowned coastlines simply by purchasing the special edition 100 Anniversary pack and scanning the QR code. Other instant win items include Corona merchandise, coolers, towels, and digital vouchers. To access the Beach 100 list – developed in partnership with experiential marketing agency, WINK – and to learn more about how to win Beach 100 prizes, visit Corona.com for more information. To kick-off the anniversary in grand fashion, Corona will take over one of the most iconic beaches in the world – Copacabana Beach in Rio De Janeiro – as a marquee sponsor of a global concert that will transform the shores of Copacabana Beach into the ultimate celebration. Taking place on May 3rd, the concert will captivate millions of attendees on the beach where each year the concert will feature some of the world’s most popular musical artists and bands. From its origins in Mexico in 1925, Corona has been dedicated to quality with an emphasis on how the beach lifestyle positively impacts consumers worldwide. Named the World’s Most Valuable Beer Brand in 2024, Corona has turned every sip into a moment of connection through its premium products – brewed with 100% natural ingredients including its iconic lime ritual – and continues to bring more 'This is Living' moments of social connection and relaxation into the world. To highlight those 100 years of beach culture, Corona has unveiled a branded legacy film, titled 'This is Living Since 1925,' that brings to life many decades unfolding over the course of a day. From the early beginnings of beach side gatherings during the bikini era to swimming and surfing across the globe today, the archival look and feel across the film sets the tone for each decade, which also depicts a variety of beaches and coastlines from around the world. As seen in the film – created in partnership with Wieden+Kennedy Amsterdam – Corona’s integration within the beach culture is effortless in the scenes over time, a testament to the brand’s natural place on the beach 'Since 1925.' The 360 platform also features vibrant new digital, trade, and OOH assets that highlight how Corona has remained a staple for beach lovers from around the world for generations — no matter the season or location. The centennial celebrations will continue throughout 2025, so grab a lime, raise a Corona, and join the brand in commemorating a century of vibrant beachside living. Cheers to 100 years under the sun and to the next century of unforgettable 'This Is Living' moments. a list of the world’s top 100 beaches and a multi-year sponsorship of a concert at Copacabana Beach in Rio de Janeiro—beginning May 3 with a Lady Gaga show.","type":"text"},{"_id":"5ZW2SIEJ4FE3DAP6E5SFNUDI6Y","additional_properties":{},"content":"The beer brand worked with experiential marketing agency Wink on the beach curation part of the project free divers and other nature enthusiasts to curate the list rating each beach based on beachside culture connection to nature and scenic aesthetics.","type":"text"},{"_id":"27ZC5IZHJFHQ5P4WBLRCTHIDOQ","additional_properties":{},"content":"The list includes well-known hot spots in South Africa as well as lesser known shorelines such as Chile’s Punta de Lobos Corona is also set to sponsor concerts at Copacabana Beach in Brazil The 100th anniversary concert will take place May 3 featuring Lady Gaga The free show launches “Todo Mundo no Rio,” a new cultural initiative in Rio.","type":"text"},{"_id":"E6Z4C25KDVAODDM7S5I2EJYTCI","additional_properties":{},"content":"“For 100 years Corona has fully embodied the ‘This is Living’ spirit of the beach lifestyle inspiring people to disconnect from their everyday routine and reconnect with nature and each other,” said Clarissa Pantoja “Enjoyed by millions around the world today and still growing Corona will always remain unwavering in its commitment to the beach This anniversary is not just a celebration of our legacy He was previously editor in chief of the Clio Awards and Muse by Clio and has also served as creative editor at Adweek.","slug":"/author/tim-nudd","native_app_rendering":false,"fuzzy_match":false,"contributor":false,"status":true,"last_updated_date":"2025-03-31T20:40:15.144Z","role":"Creativity Editor"}}}]},"description":{"basic":"Wink helped to build the global Corona Beach 100 experience backed by a W+K Amsterdam film."},"display_date":"2025-04-29T12:15:13.41Z","distributor":{"category":"other","mode":"custom","name":"crain"},"editor_note":"1 Disregard subheadline field in article template.\n2 Left panel in sepia tone with \"THIS,\" center in soft color with \"IS LIVING,\" and right in modern photo quality with \"SINCE 1925,\" set against various beach backgrounds.","auth":{"1":"43359b663491bb57d4e66a052d55dde4a0f5119819ea30a7fde901c762197125"},"caption":"Wink helped to build the global Corona Beach 100 experience He previously covered the private equity industry as a reporter for PEI Media Lindsay Rittenhouse is a senior reporter for Ad Age covering broad advertising industry trends He was previously a freelance journalist and podcaster covering pop culture and entertainment as well as a Pilates instructor and a professional dancer the “Scream” franchise and Halloween costumes covers household and personal-care marketers He's based near Cincinnati and has previously written for the Atlanta Journal Constitution woodworking and graphic design industries and worked in corporate communications for the E.W Gillian Follett is a general assignment reporter for Ad Age. She writes about a variety of topics including social media influencer marketing and the creator economy Gillian graduated from Syracuse University’s S.I He previously covered corporate communications and public relations agencies as a reporter at PRWeek Jon Springer covers sports marketing and beverage marketing He formerly covered the food retail industry for Winsight and Supermarket News and is a former sports and features writer for The Cecil Whig He has worked in newspapers from Albany to New York City He has also worked at every advertising industry trade publication that matters and he once visited Guatemala and once rode the Budapest Metro Adrianne Pasquarelli is a senior reporter at Ad Age She is also a host of the Marketer’s Brief podcast and spearheads special reports including 40 Under 40 and Hottest Brands Pasquarelli joined Ad Age in 2015 after writing for Crain's New York Business where she also focused on the retail industry.  Bradley Johnson is Ad Age's director of data analytics Johnson focuses on data and financial topics related to marketing Los Angeles and New York including editor at large overseeing breaking news and daily coverage He also contributes reporting on the beverage automotive and sports marketing industries He is a former reporter for McClatchy newspapers where he covered business and state government and politics Jones Krahl is U.S. head of creative and creative fellow at Deloitte Digital She was previously creative editor at Campaign US and also served as a writer and reporter at Sidekick by Morning Brew and PRWeek CHICAGO, April 09, 2025 (GLOBE NEWSWIRE) -- Corona Premier the only lighter than light beer worthy of being called Corona announces its partnership with Grammy-nominated artist & entrepreneur Saweetie to celebrate National Pickleball Month and the launch of their Dink N’ Drink™ activation As part of the brand’s broader Premier Side of Light campaign Dink N’ Drink™ provides an interactive post-game experience for players who seek to extend their active lifestyle giveaways – and even a new signature courtside drink recipe To bring the Dink N’ Drink campaign to life, Corona Premier teamed up with Break the Love a next-generation racquet social sports platform from Break Sports to host an exclusive pickleball event in New York City on April 23rd designed to connect players of all skill levels Whether you’re a seasoned pro or just picking up a paddle for the first time this event invites guests to get comfortable on the court meet new friends and enjoy surprise giveaways and special activities Founded by renowned beverage innovators Cody and Camille Goldstein Muddling Memories has been behind some of the most viral drink sensations transforming the industry and establishing itself as one of the most sought-after creative agencies “As a performer and someone who grew up playing sports I’ve always found it important to celebrate your wins and that’s exactly what this event is about,” said Saweetie “I’m so excited to team up with Corona Premier to play some pickleball and make this experience even more special for fans and players With pickleball’s popularity soaring – there were an estimated 19.8 million players in the U.S marking a 45.8% increase from 20231 – it’s clear that enthusiasts are drawn to more than just the competition The sport’s accessibility and inherent social nature make it the perfect match for Corona Premier a brand that celebrates the social side of living an active lifestyle by bringing people together over a premier light beer after the game we wanted to create a way for players to celebrate post-game,” said Rob Nelson “Dink N’ Drink is about elevating the best parts of the game – friendly competition extending the moment with friends after a match with a crisp Corona Premier We’re excited to be part of National Pickleball Month with a toast to the sport’s growing community.” And for those who can’t join the event in NYC Corona Premier is giving fans a chance to bring the Dink N’ Drink experience to their home court By entering the Corona Premier Dink N’ Drink Sweepstakes 25 lucky winners will have a chance to win a special Corona Premier Dink N’ Drink Kit complete with pickleball gear and all the ingredients† needed to enjoy this season’s coolest drink with friends (†no alcohol is awarded with prizes; drink ingredients awarded as a $30 Sponsor-specified gift card) Fans can visit @CoronaUSA to learn how to enter for a chance to win an exclusive Corona Premier Dink N’ Drink Kit NO PURCHASE NECESSARY TO ENTER THE CORONA PREMIER DINK N’ DRINK SWEEPSTAKES Starts 12:00 AM ET on 4/9/25 and ends 11:59 PM ET on 4/30/25 Void outside the Eligibility Area and where prohibited see Official Rules at rules.dja.com/dinkndrink Corona Premier offers drinkers an exceptionally crisp premium light beer experience smooth finish and in-the-moment beach vibes that come with Corona ​Corona Premier is perfect for casual entertaining or for those looking to extend any unwind moment Corona encourages consumers to enjoy its products and drink responsibly About the Corona Brand Family The Corona brand family is home to Corona Extra Corona Non-Alcoholic and Corona Sunbrew Citrus Cerveza The entire Corona portfolio is brewed in Mexico by Constellation Brands and imported and marketed exclusively to the U.S the flagship brand of the Corona brand family is a pilsner-style lager with a golden hue that was first brewed in Mexico in 1925 Corona embraces the Mexican beach mindset with “La Playa Awaits” and encourages consumers to live life more presently no matter where they are The refreshing flavor and carefree attitude of Corona Extra holds a respected place in U.S 1Source: SFIA Top Line Participation Report for 2025 Contact:Constellation BrandsStephanie McGuanestephanie.mcguane@cbrands.com https://www.globenewswire.com/NewsRoom/AttachmentNg/84c8b110-8a2f-4705-9e84-81c4ef154575 https://www.globenewswire.com/NewsRoom/AttachmentNg/e99e3bf3-4639-408d-ba93-fca395ca893c https://www.globenewswire.com/NewsRoom/AttachmentNg/8c445f4e-b32a-4904-8022-046d5a6d7296 https://www.globenewswire.com/NewsRoom/AttachmentNg/21d6119c-a2df-4736-978e-823384e76084 AB InBev has officially opened the eco-protected luxury destination it calls “Corona Island” to the public which was originally debuted by AB InBev in 2021 as an invite-only getaway located 20 km (12 mi) southwest of Cartagena The destination has been carefully positioned by the beer giant as “symbolic of the brand’s dedication to live in harmony with nature” and offers the chance to “disconnect from daily life and reconnect with nature” Corona Island is also the world’s first and only island to receive Oceanic Global’s three-star plastic-free Blue Seal for eliminating single-use plastics and adopting sustainable operating best practices at scale Corona Island features 10 premium waterfront bungalows nestled amongst tropical forests and beaches The island also offers all-inclusive overnight stays for two people per bungalow alongside a limited number of all-inclusive day trip passes that are also available to buy global vice president of Corona said: “The inspiration to build this experience stems from Corona’s long-standing commitment to nature and is a physical manifestation of our vision for eco-friendly tourism and conservation efforts Corona now welcomes eco-conscious travellers from around the world to experience the island and truly disconnect to reconnect with nature.” and traditional building techniques such as bahareque The island also serves its food with an emphasis on local ingredients such as baked goods made with fruits native to the region which are all included in the price of the visit Also available for guests to enjoy are the full suite of Corona products including Corona Extra and Corona Cero as well as a variety of relaxation and eco-conscious activities and kayaking and snorkelling to restore coral reefs and mangrove planting for ecosystem reforestation Pantoja explained: “As a beer brewed with natural ingredients Corona has always been associated with nature and relaxation Corona Island is an extension of that ethos – offering an experience that blends leisure with eco-friendly travel experiences.” relaxation meets conservation in a natural beachside setting and a beer brewed with natural ingredients we believe that our island serves as a perfect extension of our ethos.” we invite eco-tourists and travel enthusiasts alike to explore this unique setting where every experience reflects our dedication to preserving the beauty of the natural world.” Corona Island is priced at US$600 per night and US$200 per day-pass – both are all-inclusive the individual booking sites will provide all prices in the local currency for each potential traveller for ease Travellers worldwide can secure their stay directly at livecoronaisland.com or through Airbnb We are using cookies to give you the best experience on our website You can find out more about which cookies we are using or switch them off in settings This website uses cookies so that we can provide you with the best user experience possible Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful Strictly Necessary Cookie should be enabled at all times so that we can save your preferences for cookie settings we will not be able to save your preferences This means that every time you visit this website you will need to enable or disable cookies again Please select what you would like included for printing: Copy the text below and then paste that into your favorite email application Add to Calendar Complete the form below to get directions for the Funeral Service for Sherry Corona Complete the form below to get directions for the Visitation one hour prior to the service for Sherry Corona This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply Service map data © OpenStreetMap contributors 2025Jared CornickPrep Baseball California Contributor - Coming into this season being defending Prep Baseball National Champions the expectations are straightforward for Coach Andy Wise and Corona High School “Our expectations are to be the best and win everything,” said Andy Wise head coach of the Corona Panthers since 2009 Wise has taken the high school baseball world by storm with him and his staff’s ability to construct what is to be considered one of the best baseball programs in the entire nation the team may even be better this year than it was the year prior “This group is just trying to repeat what that team did but that does not dictate winning,” said Wise The talent of this roster is evident on paper With a lineup containing eight Division One committed players the Panthers are a team forced to be reckoned with It starts with three highly touted seniors and all two time Prep Baseball All-Americans: Seth Hernandez Seth Hernandez is the number one ranked pitcher and number two ranked player in the class of 2025 The 6’ 3’’ dual threat can top 100 MPH on the mound with an exit velocity off the bat reaching upwards of 107.1 MPH Hernadez serves as the team’s ace as well as a pivotal bat in the lineup Brady Ebel a SS committed to play at LSU is the number two-ranked infielder and the number four ranked player in the class of 2025 five-tool prospect has a career OBP of .368 for the Panthers as well as playing exceptional defense at both 3B and SS Billy Carlson a SS/RHP committed to play at the University of Tennessee is the fifth-ranked shortstop and the seventh-ranked player in the class of 2025 A former Prep Baseball Future Games participant Carlson hit to the tune of a .367 average in his junior season for Corona as well as clubbing four home runs in the process Carlson serves as a vital asset in Corona’s bullpen As for coaching such highly touted prospects Coach Wise finds it to be pretty easy due to how they take care of their business “Those guys know one way to play the game both mentally and physically and they just do it," said Wise but most importantly they are great people.” In addition to Carlson, Ebel, and Hernadez, the Corona lineup and rotation is bolstered by the likes of other division one commits such as ‘25 RHP/OF Jason Gerfers (Arizona State) and ‘25 OF Joshua Sur (Vanguard) Leadoff hitter ‘26 OF Anthony Murphy is committed to play at Louisiana State University as well Brady’s younger brother, INF Trey Ebel is committed to play at Texas A&M in the fall of 2026 ‘25 RHP/INF Ethin Bingamin is committed to Auburn University and will join Ebel and Carlson in the ever-so-competitive SEC We are just going to do what we do and our lineup is extremely difficult on pitchers," said Wise "It’s all about grinding out good-at bats and every guy from top to bottom is a threat.” The pitching staff is just as equally talented as the bats that Corona puts forth and a deep reliever pool highlighted by Carlson and Gerfers Corona’s well-rounded roster provides a tough test for any team that faces the Panthers “We probably have the best pitching staff and we probably have the best defense going,” said Wise Wise and his staff have always prioritized a schedule that provides a challenge The Panthers face competitive schools such as Mater Dei and Summit High School this season to help them prepare for what they expect to be a deep playoff run “Even when we were not highly touted and ranked the philosophy of mine and this program is to be big game hunters and beef up our schedule," said Wise "We want the best game we can get so we can take something from each good program we play and add it to our own mix.” The culture that Wise has fostered at Corona has been a long time in the making a former player at Long Beach State and Santa Ana College has absorbed the knowledge of what it takes to be an excellent coach from the likes of Dave Snow and Ken Ravizza “I am really just trying to give my spin with my personality from the coaches that I have played for and worked with," said Wise "Corona Baseball is a little bit of Santa Ana College and I couldn't be more honored to represent those programs and those coaches Just trying to do the same as they did for me.” Panther alumni is headlined by the likes of Joe Kelly and Tristian Beck a two-time world series champion with the Los Angeles Dodgers went on to play for UC Riverside and was drafted by the St Louis Cardinals in the third round of the 2009 MLB June Amateur Draft a right-handed pitcher for the San Francisco Giants attended Stanford University after his time at Corona and was originally drafted by the Atlanta Braves in the fourth round of the 2018 MLB June Amateur Draft “It’s a great brotherhood and having alumni come back and share their stories is amazing," said Wise "All the way from Joe Kelly to Tristian Beck you can really see how the Corona baseball community and culture has grown and it's fun to watch to see players who have represented our program enter the real world prepared the Corona Panthers hope to run it back and prove why they are considered to be one of the Corona Panthers have what it takes to be repeat as Prep Baseball National champions “We know everyone wants to kick our ass so we’re always getting a team’s best and we wouldn’t want it any other way,” said Wise + Prep Baseball California on X+ Prep Baseball California on Instagram+ Prep Baseball California on Facebook+ Prep Baseball California on YouTube           Heritage Wellness Collective won a revocable license to run the market for the next five years following a 6-1 vote by the city council at their meeting Tuesday, with Councilman Erik Weigand as the only member in opposition. The nonprofit will take over after the license with the current manager, Rick Heil, expires on June 30. The city began soliciting bids for a new operator in August, according to a staff report. Newport Beach officials made their decision based on candidates’ experience running farmer’s markets as well as their ability to create community events and attract quality merchants while retaining existing vendors. Heritage Wellness Collective was chosen out of 8 bidders. The nonprofit runs seven weekly or bi-monthly farmer’s markets in Orange, Los Angeles, San Bernardino and Riverside counties. Their executive director, Bing Turner, has a background in public health, and told the Daily Pilot during an interview Wednesday he hopes to preserve the Corona Del Mar gathering’s focus on high quality produce while drawing in more families, local businesses and institutions to visit and take part in it. “Will the market look different than what it currently looks like now? Absolutely yes,” Turner said. “Will the vendors change at the market? That’s going to happen. Are we going to move people out of their market or out of their spaces? No, unless they decide they don’t want to work with us. But we’re there to really enhance and make the space reflective of the community... this isn’t going to be any rock concert.” Numerous residents who attended Tuesday’s meeting said they feared the merchants supplying their favorite carrots, strawberries, pomegranates, flowers, cuts of meat and more may go away if the market changes management. Current operator Heil claimed that only one of its current vendors had been contacted by Heritage. Several public speakers cited the adage “if it ain’t broke, don’t fix it.” They said they preferred that the the market remain solely for the exchange of artisan produce. Many worried that adding food vendors, music and public events would make it a larger event that would result in more noise and traffic. Opponents of the change in vendors claim more than 1,000 people had signed a petition to prevent the move. “The people are really angry in the community,” Heil told the Pilot at the most recent Corona Del Mar Farmer’s Market on Saturday. “I’ve never seen anything like this where the city council go up against the will of the people who like it and don’t want to change something that’s been there 29 years.” However, Turner said he has heard from numerous people in the Newport Beach community who want to see the market evolve into a more family-oriented event. He said that includes local chefs and merchants who had been denied after expressing interest in doing business at the market. Turner also refuted Heil’s claim that only one vendor had been contacted by Heritage. The nonprofit’s executive director said emails were sent prior to Tuesday’s meeting to about 15 or 20 merchants currently doing business at the Corona Del Mar market, and 14 had replied expressing interest in staying under new management. Turner added that he was hesitant to contact them before confirming the new license agreement, but had been advised by city staff to do so. Concerned Corona Del Mar residents said that email was the first notification they received regarding new management and the city did not reach out to them for input. Many said the preliminary message from Heritage was evidence that the change was a “done deal” even before council members cast their votes. “We like the Farmers Market the way it is,” said Corona del Mar resident and petition monitor, Carole Geronsin said Saturday. “Everyone we talked to there was not one person that doesn’t absolutely love it the way it is.” Turner, as well as members of the council, acknowledged that more could have been done to engage residents before the issue came to council. However, he said Heritage’s immediate priority will be building a bridge with the Corona Del Mar Community to ensure the market continues to meet their needs and wants, while growing to include more of their neighbors. Before voting on the new agreement, Councilman Weigand asked that provisions for the inclusion of live music be removed. Mayor Pro Tem formally backed that amendment, which was seconded by Councilman Noah Blom. Councilmembers also asked city staff to further study potential impacts to traffic. “I get it they don’t want to lose something they have none of us do once we have it... nobody on this dais ever wants to see food trucks everywhere,” Blom said. “I’m here to give new things a try because sometimes it’s amazing what happens when we take a chance on something new. And I hear the scoffing from people in the room, mainly from the current operator.” Blom and other councilmembers also noted that the new license is revocable, and can be terminated if new management does not prove satisfactory. Eric Licas covers Newport Beach for the Daily Pilot. He previously was a crime and public safety reporter and, before that, spent four years as a staff writer with the Orange County Register and the Southern California News Group. He has been on the ground to cover active wildfires, civil unrest and mass shootings. He was born in the Philippines, raised in the San Fernando Valley and is a Cal State Northridge alumnus. Susan Hoffman is a contributor to Times Community News. News Subscribe for unlimited accessSite Map  Corona Henrietta Bucher “Coonie” Essner at Saint Francis Medical Center in Cape Girardeau daughter of the late Philip and Leocadia Dirnberger Bucher Her life was marked by unwavering dedication to her faith as she served in various capacities throughout her life she moved from the family farm in New Hamburg to Cape Girardeau where she was an active member of St Vincent de Paul Catholic Church and the Cathedral of St She worked at Cape LaCroix Apartments in Cape Girardeau Lawrence Parish School of Religion and in the church choir She brought the first Teens Encounter Christ (TEC) conference team to the Diocese of Springfield-Cape Girardeau many summers at Camp Re-NEW-all in Fredericktown Coonie was also active in the regional and Diocesan Council of Catholic Women and the Oran KC-ettes She enjoyed visiting friends in nursing homes giving rides to those in need and making time for her ladies’ groups which included the New Hamburg Homemakers Club Red Hat Society and her friend group which met regularly over the past 50 years to celebrate their birthdays she also volunteered for 20 years in the gift shop at St receiving the Volunteer of the Year Award in 2018 When she completed her last day of service on Feb Known affectionately as "Nana" to her grandchildren creating lasting memories through card games and her famous homemade pumpkin bread which her grandchildren looked forward to receiving every Christmas along with their nylon pot scrubbers She was always up for any adventure and there to listen and lend a hand when needed Survivors include: two sons and a daughter-in-law and Tim Essner of New Hamburg; daughter-in-law Texas; Dustin (Amy) Essner of New Hamburg; Kari (James) Wilhelm of Kelso; Kent Essner of Dexter; Leonna (Dean) Heuring of New Hamburg; Michelle (Andy) Silman of Benton; Andrea (Pete) Sampanis of Vienna Virginia; and Lisa (Jim) Eftink of New Hamburg; 21 great-grandchildren; one great-great-grandson; and many nieces A funeral Mass will be celebrated at 10 a.m Lawrence Catholic Church in New Hamburg with the Rev Pallbearers will be her great-grandsons: Drew Silman Honorary pallbearer will be her great-grandson Memorial contributions may be made to SSM Health Cardinal Glennon Children’s Foundation at glennon.org or St Enter your phone number above to have directions sent via text tourists will be able to immerse themselves in a Corona experience by holidaying on an island dedicated to the lager adopting it for the use of “friends and family of the brand,” according to its vice-president the world was in the grip of the similarly named Coronavirus the paradise has been opened up to public bookings via Airbnb Corona Island sits 12 miles off the coast of Cartagena Its isolation means it needs to offer an all-round hospitality experience and It’s badged with marine conservation charity Oceanic Global’s three-star plastic-free Blue Seal for eliminating single-use plastics making it the first and only island to do so solar energy powers the operation and traditional building techniques such as bahareque have been employed alongside the use of native plants such as guadua in addition to well-stocked supplies of Corona fresh local food and experiences that include yoga and stand-up paddle boarding You can also take part in snorkeling coral reef restoration projects and planting mangroves known for their carbon-capturing properties “It’s more than just relaxing; it’s remembering how important it is to live in harmony with nature and the programs you can choose from include making food with natural ingredients which helps connect us to the natural world,” says Pantoja While the experience is choreographed to blend sustainable practices with a reconnection to the natural world Pantoja is also keen to link this back to the product which is “brewed with 100% natural ingredients,” she says is an example to the world of what we believe in as there’s nothing more natural than protecting and being connected to nature,” she adds Corona is all in with the endeavor and although it hasn’t bought the island so it does very much belong to the company – for now drawing attention to sustainably minded endeavors should really be a chance for a company to illuminate its attitude to environmental social and governance (ESG) as a whole rather than demonstrate one project in isolation The actions that will really dictate the environmental impact of Corona and its parent company take place across the whole lifecycle and supply chain of the product Corona was not able to provide any figures on further efforts the brand is making to reduce its environmental impact in particular around any journey towards net zero or the environmental impact of its factories However, since 2021, it has achieved a net zero plastic footprint by recovering more plastic from the environment than it uses Pantoja also reminds us that all of its bottles have always been glass with only the ingredients label on the back made of plastic and that the brand has used engraved bottles for 100 years She also answered that all-important question: lime in the top of the bottle or no lime Catch up on the most important stories of the day Stay up to date with a curated digest of the most important marketing stories and expert insights from our global team Learn how to pitch to our editors and get published on The Drum An official website of the United States government Select a service on this page to check the hours Wheelchair availability:  Wheelchairs are available upon arrival for patients who need them to access the building We work with Disabled American Veterans and county Veterans Affairs directors to provide transportation for Veterans and authorized caregivers to get to scheduled medical appointments Many localities in the region provide other van services to Veterans Learn more about DAV and the other van services available in your county Corona Public Transit Beneficiary travel benefits include round-trip transportation from your home to the medical center Find out if you qualify for beneficiary travel benefits Our laboratories provide a full range of clinical and diagnostic testing services Our laboratory and pathology services include: Our medical center and clinics provide consultation and treatment for a range of issues that may impact your mental health or emotional well-being Our confidential outpatient services include individual and group therapy for: Learn more and connect with a care coordinator My HealtheVet is a web-based tool you can use to manage your care and improve your health from your computer or mobile device You can access your personal health records and use tools to manage your care through our My HealtheVet online system You can also use My HealtheVet to refill your prescriptions you can contact our coordinator by phone or in person Learn more and register for My HealtheVet Our pharmacy provides you and other Veterans with convenient efficient service to make it easy to get your medications and medical supplies Learn more about our pharmacy Your VA primary care provider will work closely with you to plan for all the care you need to stay healthy and well throughout your life They will also work with family members or caregivers who support you A strong network of family and internal medicine specialists and services can offer you the best possible care Internal medicine doctors (internists) prevent Doctors who specialize in family medicine provide primary health care to the entire family Your primary care team can coordinate the many services you receive such as: NOTICE TO PATIENTS: Some of our medical doctors are licensed and regulated by the Medical Board of California. To check up on a license or to file a complaint go to https://www.mbc.ca.gov/, email: licensecheck@mbc.ca.gov Deportation threats and the potential of job losses have led to decreased spending among Hispanic consumers who make up half of the company’s beer sales Part of Constellation’s strategy to overcome difficulties in the beer market is to better advertise Modelo beyond the Hispanic market “We increased our [marketing] spend even though the consumer was pulling back and we’re seeing that providing great returns to our business,” Newlands said TD Cowen analyst Robert Moskow pointed to Nielsen data that found the rate of Constellation beer purchases among Hispanic consumers declined between 7% and 9% so far in 2025 He projected immigration and employment issues related to the demographic could weigh on the company for years to come Constellation's CEO pointed to internal data which found two-thirds of Hispanic consumers are concerned about the price of food Half are worried about Trump’s actions on immigration an area where the Hispanic consumer often consumes beer are declining today as part of these overarching concerns they have,” Newlands said “We’re going to need and want to see some improvement in the consumer brand health before we’re able to correctly project how long some of these challenges are going to last.” Constellation lowered its anticipated sales growth for its beer business to 0% to 3% In its most recent financial quarter ending in February, the company’s beer business saw flat growth in net sales and a 1.8% decline in shipments, according to Constellation’s earnings report Declining demand is just one issue linked to Trump’s policies that has weighed down the Pacifico brewer. Constellation is considered among the brands most vulnerable to the president's tariff policy because imports make up nearly 85% of its annual revenues Correction: A previous version of this story misidentified the owner of Modelo and Corona which are distributed by Constellation Brands in the U.S Get the free daily newsletter read by industry experts The soda giant’s Red Tree Beverages subsidiary is making a bet that its vodka lemonade can help it gain a stronger foothold in booze Ingredion and consumer insights platform Tastewise say innovative tech can help companies stay ahead of the latest trends and potential supply chain headwinds The free newsletter covering the top industry headlines 2025Investigators are searching for an arson suspect who they say caught on fire while he was setting a car ablaze in Corona (KABC) -- Investigators are searching for an arson suspect who they say caught on fire while he was setting a car ablaze in Corona - an incident that was caught on video It happened early Tuesday morning in the front yard of a home on Wakefield Avenue Surveillance footage shows the man standing next to a black sedan before flames suddenly erupt He is then seen catching fire as well before appearing to jump a fence and fleeing the scene while on fire The fire destroyed the car and caused some damage to the nearby home Authorities believe the suspect got away in a newer model white pickup truck with tinted windows and light-colored rims Anyone with information is urged to call Corona fire investigators at (951) 736-22-119 Research Development and Administration Team If the price tag on your Modelo or Corona is higher next year Many economists say that the 25% tariffs President-elect Donald Trump has proposed on imported goods from Mexico and Canada not to mention additional tariffs on Chinese goods would be catastrophic for the economy and consumers an assistant teaching professor of history at Northeastern University whose focus is the history of beer says they will hit those beer brands particularly hard “The most popular beer brand in the entire country right now is Modelo,” Purinton says “The thing is the demand isn’t going to go away it’s going to dramatically affect the costs of those.” Read more on Northeastern Global News. The College of Social Sciences and Humanities combines Northeastern University’s signature focus on experiential learning with the rigorous study of society CSSH is redefining liberal arts education for the next generation of global thinkers Your support and partnership can help CSSH and our students continue to lead CSSHDean@northeastern.edu Metrics details The protein corona formed on nanoparticles (NPs) has potential as a valuable diagnostic tool for improving plasma proteome coverage and nutrients into plasma can induce diverse protein corona patterns on otherwise identical NPs significantly enhancing the depth of plasma proteome profiling The protein coronas on polystyrene NPs when exposed to plasma treated with an array of small molecules allows for the detection of 1793 proteins marking an 8.25-fold increase in the number of quantified proteins compared to plasma alone (218 proteins) and a 2.63-fold increase relative to the untreated protein corona (681 proteins) we discovered that adding 1000 µg/ml phosphatidylcholine could singularly enable the detection of 897 proteins phosphatidylcholine selectively depletes the four most abundant plasma proteins thus reducing the dynamic range of plasma proteome and enabling the detection of proteins with lower abundance Employing an optimized data-independent acquisition approach the inclusion of phosphatidylcholine leads to the detection of 1436 proteins in a single plasma sample Our molecular dynamics results reveal that phosphatidylcholine interacts with albumin via hydrophobic interactions The addition of phosphatidylcholine also enables the detection of 337 additional proteoforms compared to untreated protein corona using a top-down proteomics approach Given the critical role of plasma proteomics in biomarker discovery and disease monitoring we anticipate the widespread adoption of this methodology for the identification and clinical translation of biomarkers Peptides from these high-abundance proteins tend to dominate mass spectra impeding the detection of proteins with lower abundance utilizing a single type of NP can streamline the MS analysis process reducing the time required to analyze large cohorts in plasma proteomics studies to assess the potential collective effects of these molecules we analyzed two representative “molecular sauces.” Molecular sauce 1 contained a blend of glucose employing a wide range of characterizations to analyze the protein corona of polystyrene NPs This rigorous optimization ensures highly accurate and reproducible results Our findings confirm that the addition of these small molecules in plasma generates distinct protein corona profiles on otherwise identical NPs significantly expanding the range of the plasma proteome that can be captured and detected by simple LC-MS/MS analysis we discover that the addition of specific small molecules leads to a substantial increase in proteome coverage which is attributed to the unique ability of PdtChos to bind albumin and reduce its participation in protein corona formation PtdChos coupled with NP protein corona analysis can replace the expensive albumin depletion kits and accelerate the plasma analysis workflow by reducing processing steps our single small molecule-single NP platform reduces the necessity for employing multiple NP workflows in plasma proteome profiling This approach can seamlessly integrate with existing LC-MS/MS workflows to further enhance the depth of plasma proteome analysis for biomarker discovery We assessed the effect of eight distinct small molecules, namely, glucose, triglyceride, diglycerol, PtdChos, PE, PtdIns, IMP, and vitamin B complex, on the protein corona formed around polystyrene NPs. The workflow of the study is outlined in Supplementary Fig. 1 a The number of quantified proteins in plasma and protein coronas in the presence of small molecules and molecular sauces (mean ± SD of three technical replicates) The cumulative number of unique proteins identified using untreated protein corona and corona treated with various small molecules is also shown using the purple bar the database was performed individually for each small molecule (the higher the small molecule(s) concentration b The distribution of averaged normalized abundances of three technical replicates for proteins quantified in the plasma and protein coronas in the presence of small molecules and molecular sauces (the higher the small molecule(s) concentration the darker the blue shade; boxplot: center line median; box limits contain 50%; upper and lower quartiles greatest value excluding outliers; minimum more than 1.5 times of upper and lower quartiles) c Clustered heatmap of the normalized abundance of all 1793 proteins quantified across all samples d Clustered heatmap of the normalized abundance of 117 shared proteins across all samples Experiments were performed in three technical replicates To mitigate the impact of these variables on the interpretation of how small molecules can enhance proteome coverage we chose to report our data as fold changes in the number of quantified proteins relative to control plasma and untreated corona samples This approach offers a more objective assessment of the role of small molecules in enhancing proteome analysis minimizing the confounding effects of different workflows and data analysis techniques that may be employed by various researchers While most of the enriched pathways were shared some pathways were specifically enriched for a given molecular sauce systemic lupus erythematosus (SLE) was only enriched among the top pathways for molecular sauce 2 the small molecules can be potentially used for facilitating the discovery of biomarkers for specific diseases or for assaying the abundance of a known biomarker in disease detection To understand whether the quantification of a higher number of proteins in protein corona profiles was due to a lower dynamic range of proteins available in human plasma for NP binding, we plotted the maximum protein abundance vs minimum protein abundance for plasma alone, and plasma-treated with small molecules in Supplementary Fig. 10 The plasma alone showed the highest dynamic range suggesting that identification of low-abundance proteins would be most difficult from plasma alone the addition of small molecules was shown to reduce plasma protein dynamic range thereby allowing for the detection of more peptides and quantification of proteins with lower abundance through the NP protein corona a Normalized protein abundance (left axis bar plot) and protein rankings (right axis b A stream (or alluvial) diagram illustrating the significant depletion of abundant plasma proteins following the incubation of plasma with NPs and PtdChos (only shared proteins with plasma are included; colors are chosen randomly) c Total count of proteins identified in plasma and protein corona treated with PtdChos at various concentrations (colors are chosen randomly; mean ± SD of three technical replicates) d A stream diagram demonstrating the depletion pattern of abundant plasma proteins in response to NP addition and enhanced with escalating concentrations of PtdChos (colors are chosen randomly; only shared proteins with plasma are included) allowing for more robust detection of other proteins with lower abundance These findings not only validate the enhancement of plasma proteome coverage by PtdChos but also illustrate the capability of PtdChos to facilitate the in-depth profiling of the plasma proteome associated with protein corona formed on the surface of a single type of NP Since the ratio of the number of quantified proteins through PtdChos spiking is generally around 1.4-fold higher than in the NP corona alone PtdChos can be incorporated into any LC-MS workflow aiming to boost plasma proteome profiling More optimized plasma proteomics pipelines or high-end mass spectrometers such as Orbitrap Astral are envisioned to quantify an even higher number of proteins than those reported in the current study In total, 637 proteoforms were identified across the two samples (with technical triplicates for each sample) (Fig. 3c) Data analysis using Perseus software (Version 2.0.10.0) revealed that only 110 proteoforms overlapped between the two samples (the minimum number of valid values for filtering data was set to 1) The proteoform mass distribution differed between the two samples (Fig. 3d) Although the average proteoform masses were similar the box plot indicated a greater number of larger proteoform identifications in the control sample (over 20 kDa) We hypothesize that PtdChos can bind to large proteins and due to the high concentration of PtdChos relative to the proteoforms the signals of these large proteoforms may be obscured Additional data analyses identified differential proteins in this study (Fig. 3e) The top-down proteomics approach identified specific gene products that bind to the NP surface in the presence of PtdChos a Total linear interaction energies between albumin and various number of ligands systems over simulation time The total energy represents the sum of Lennard-Jones and Coulombic energies b Effective free energy of binding terms for the different systems over the entire simulation GGAS represents the energy of the gas phase c Average root mean square fluctuation of albumin residues for the four systems d Root mean square deviation of PtdChos over time for the four types of systems e Bond types present within each simulation and the x-axis represents the percentage of simulation timestamps when each type of bond is present The results of our molecular dynamics evaluations of the interactions between PtdChos and albumin were in line with the literature we found that the simple addition of PtdChos to plasma can significantly reduce albumin adsorption for the surface of polystyrene NPs thereby creating unique opportunities for the involvement of a broader range of proteins with lower abundance in the protein corona layer We also observed the same effects of PtdChos on enhancing the proteome coverage using different types of NPs Not only is PtdChos an economical and simple alternative for conventional albumin depletion strategies but it can also deplete several other highly abundant proteins as an added advantage This approach reduces the necessity for employing NP arrays in plasma proteome profiling and the cost and biases that can occur with albumin depletion PtdChos can help accelerate plasma analysis workflows by reducing the sample preparation steps Our study highlights the tremendous potential of leveraging small molecules to enhance the capabilities of protein corona profiles for broader plasma proteome analysis By introducing individual small molecules and their combinations into plasma we have successfully created distinct protein corona patterns on single identical NPs thereby expanding the repertoire of attached proteins we quantified an additional 1573 unique proteins that would otherwise remain undetected in plasma This enhanced depth in protein coverage can be attributed to the unique interactions of each small molecule allowing for the representation of a diverse set of proteins in the corona our findings underscore the influence of small molecules on the types and categories of proteins in the protein corona shell This feature opens exciting possibilities for early disease diagnosis particularly in conditions such as cardiovascular and neurodegenerative disorders our study demonstrated that PtdChos preferentially interact with highly abundant plasma proteins thereby reducing their binding to NP surfaces This reduction allows low-abundance proteins to contribute more significantly to the protein corona profile This effect was achieved by reducing the binding of highly abundant proteins and enhancing the representation of low-abundance proteins on the NP surfaces We acknowledge that the number of human plasma samples used in this study was limited primarily due to our specific focus on improving proteome coverage through the use of a single pooled plasma sample This approach effectively allows us to test and validate our hypothesis given that the most abundant plasma proteins exhibit minimal variability between individuals for future biomarker discovery applications it is essential to expand the sample size to a more diverse cohort This will ensure the platform fully accounts for biological variability and provides a more comprehensive and generalizable assessment of the proteome across different individuals the depth of analysis can further increase toward the ultimate goal of achieving comprehensive human proteome coverage Another alternative would be to combine our strategy with tandem mass tag (TMT) multiplexing and fractionation to achieve an even higher plasma proteome depth We anticipate that this platform will find extensive applications in plasma proteome profiling providing an unprecedented opportunity in disease diagnostics and monitoring and diluted to the desired concentration with 55% human plasma Mass spectrometry-grade lysyl endopeptidase (Lys-C) was sourced from Fujifilm Wako Pure Chemical Corporation Formic acid and C18 StageTips were purchased from Thermo Fisher Scientific For protein corona formation in the presence of small molecules individual or pooled human plasma proteins 55% were first incubated with individual small molecules or in combination by preparing two molecular sauces of individual small molecules at different concentrations (i.e. each type of polystyrene NPs was added to the mixture of plasma and small molecules solution so that the final concentration of the NPs was 0.2 mg/ml and incubated for another 1 h at 37 °C It is noteworthy that all experiments are designed in a way that the concentration of NPs To remove unbound and plasma proteins only loosely attached to the surface of NPs protein–NP complexes were then centrifuged at 14,000×g for 20 min the collected NPs’ pellets were washed three times with cold PBS under the same conditions and the final pellet was collected for further analysis we used various concentrations of PtdChos (i.e. and 10000 µg/ml) and used the same protein corona method for the preparation of the samples for mass spectrometry analysis DLS and zeta potential analyses were performed to measure the size distribution and surface charge of the NPs before and after protein corona formation using a Zetasizer nano series DLS instrument (Malvern company) A Helium-Neon laser with a wavelength of 632 nm was used for size distribution measurement at room temperature TEM was carried out using a JEM-2200FS (JEOL Ltd) operated at 200 kV The instrument was equipped with an in-column energy filter and an Oxford X-ray energy dispersive spectroscopy (EDS) system Twenty microliters of the bare NPs were deposited onto a copper grid and used for imaging 20 μl of samples was negatively stained using 20 μl uranyl acetate 1% PC composition was also determined using LC-MS/MS The collected protein corona-coated NP pellets were resuspended in 20 µl of PBS containing 0.5 M guanidinium-HCl The proteins were reduced with 2 mM DTT at 37 °C for 45 min and then alkylated with 8 mM IAA for 45 min at room temperature in the dark 5 µl of LysC at 0.02 µg/µl in PBS was added and incubated for 4 h followed by the addition of the same concentration and volume of trypsin for overnight digestion the samples were centrifuged at 16,000×g for 20 min at room temperature to remove the NPs The supernatant was acidified with TFA to a pH of 2–3 and cleaned using C18 StageTips The samples were then heated at 95 °C for 10 min and submitted to the core facility for LC-MS analysis The PtdChos concentration series experiment was performed using the same protocol and the samples were analyzed over a 120 min gradient The protein corona-coated NPs (with/without PtdChos) were separately treated in a 0.4% (w/v) SDS solution at 60 °C for 1.5 h with continuous agitation to release the protein corona from the NP surface the supernatant containing the protein corona in 0.4% SDS was separated from the NPs by centrifugation at 19,000×g for 20 min at 4 °C the supernatant underwent an additional centrifugation step under the same conditions The final protein corona sample was then subjected to buffer exchange using an Amicon Ultra Centrifugal Filter with a 10 kDa molecular weight cut-off effectively removing sodium dodecyl sulfate (SDS) from the protein samples The buffer exchange process began by wetting the filter with 20 µl of 100 mM ABC (pH 8.0) followed by centrifugation at 14,000×g for 10 min 200 µg of proteins were added to the filter and centrifugation was conducted for 20 min at 14,000×g This step was repeated with the addition of 200 µl of 8 M urea in 100 mM ammonium bicarbonate followed by centrifugation for 20 min at 14,000×g and repeated twice to ensure complete removal of SDS and other small molecules To eliminate urea from the purified protein the filter underwent three additional rounds of buffer exchange All procedures were carried out at 4 °C to effectively eliminate urea from the protein corona the total protein concentration was determined using a bicinchoninic acid (BCA) assay kit from Fisher Scientific (Hampton The samples were then stored overnight at 4 °C consisting of 40 µl (without PtdChos initially) and 44 µl (with PtdChos initially) of 100 mM ABC with a protein concentration of 2.8 mg/ml The RPLC separation was performed using an EASY-nLC™ 1200 system from Thermo Fisher Scientific A 1-µL aliquot of the protein corona sample (0.3 mg/mL) was loaded onto a home-packed C4 capillary column (75 µm i.d Sepax) and separated at a flow rate of 400 nL/min A gradient composed of mobile phase A (2% ACN in water containing 0.1% FA) and mobile phase B (80% ACN with 0.1% FA) was used for separation The gradient profile consisted of a 105-min program: 0–85 min The LC system required an additional 30 min for column equilibration between the analyses resulting in approximately 135 min per LC-MS analysis The experiments utilized a Q-Exactive HF mass spectrometer employing a data-dependent acquisition (DDA) method MS settings included 120,000 mass resolution (at m/z 200) parameters included 120,000 mass resolution (at 200 m/z) the top five most intense precursor ions from each MS spectrum were selected in the quadrupole and fragmented using higher-energy collision dissociation (HCD) Fragmentation occurred exclusively for ions with intensities exceeding 5E4 and charge states of 4 or higher Dynamic exclusion was enabled with a 30-s duration and the “Exclude isotopes” feature was activated Complex sample data were analyzed using Xcalibur software (Thermo Fisher Scientific) to obtain proteoform intensities and retention times Chromatograms were exported from Xcalibur and formatted using Adobe Illustrator for the final figure presentation The resulting mass spectra were stored in msalign files while proteoform feature information was stored in text files Database searches were carried out using TopPIC Suite against a custom-built protein database (~2780 protein sequences) which included proteins identified in the BUP data The search allowed for a maximum of one unexpected mass shift with mass error tolerances of 10 ppm for precursors and fragments Unknown mass shifts up to 500 Da were considered False discovery rates (FDRs) for proteoform identifications were estimated using a target-decoy approach filtering proteoform identifications at 1% and 5% FDR at the PrSM and proteoform levels The samples were centrifuged at 14,000×g for 20 min to remove the unbound proteins The collected NP pellets were washed three times with cold PBS under the same conditions The samples were resuspended in 20 µl of PBS and the proteins were reduced with 2 mM DTT (final concentration) for 45 min and then alkylated using 8 mM IAA (final concentration) for 45 min in the dark 5 µl of LysC at 0.02 µg/µl was added for 4 h followed by the same concentration and volume of trypsin overnight The samples were then centrifuged at 16,000×g for 20 min at room temperature to remove the NPs then cleaned using C18 cartridges and vacuum dried Dried peptides were resuspended in 0.1% aqueous formic acid and subjected to LC-MS/MS analysis using an Exploris 480 mass spectrometer fitted with a Vanquish Neo (both Thermo Fisher Scientific) and a custom-made column heater set to 60 °C Peptides were resolved using an RP-HPLC column (75 μm × 30 cm) packed in-house with C18 resin (ReproSil-Pur C18–AQ The following gradient was used for peptide separation: from 4% B to 10% B over 7.5 min to 35% B over 67.5 min to 50% B over 15 min to 95% B over 1 min followed by 10 min at 95% B to 5% B over 1 min followed by 4 min at 5% B Buffer A was 0.1% formic acid in water and buffer B was 80% acetonitrile The mass spectrometer was operated in DIA mode with a cycle time of 3 s MS1 scans were acquired in the Orbitrap in centroid mode at a resolution of 120,000 FWHM (at 200 m/z) and maximum ion injection time mode set to Auto MS2 scans were acquired in the Orbitrap in centroid mode at a resolution of 15,000 FWHM (at 200 m/z) quadrupole isolation window of 7 m/z with 1 m/z window overlap normalized AGC target set to 3000% and a maximum injection time of 22 ms Peptides were fragmented by HCD with collision energy set to 28% and one microscan was acquired for each spectrum The acquired RAW files were searched individually using the Spectronaut (Biognosys v18.6) directDIA workflow against a Homo sapiens database (consisting of 20,360 protein sequences downloaded from Uniprot on 2022/02/22) and 392 commonly observed contaminants For analysis of the impact of PtdChos treated plasma and different NPs we chose a quicker LC-MS setup (30 SPD) consisting of an Exploris 480 fitted with an Evosep One using the following settings Dried peptides were resuspended in 0.1% aqueous formic acid loaded onto Evotip Pure tips (Evosep Biosystems) and subjected to LC-MS/MS analysis using an Exploris 480 Mass Spectrometer (Thermo Fisher Scientific) fitted with an Evosep One (EV 1000 Peptides were resolved using a performance column—30 SPD (150 μm × 15 cm Evosep Biosystems) kept at 40 °C fitted with a stainless-steel emitter (30 um Evosep Biosystems) using the 30 SPD method Buffer A was 0.1% formic acid in water and buffer B was acetonitrile The mass spectrometer was operated in DIA mode MS1 scans were acquired in centroid mode at a resolution of 120,000 FWHM (at 200 m/z) MS2 scans were acquired in centroid mode at a resolution of 15,000 FWHM (at 200 m/z) quadrupole isolation window of 12 m/z without window overlap normalized AGC target set to 3000% and maximum injection time mode set to Auto The acquired RAW files were searched using the Spectronaut (Biognosys v19.0) directDIA workflow against a Homo sapiens database (consisting of 20,360 protein sequences downloaded from Uniprot on 2022/02/22) and 392 commonly observed contaminants Default settings were applied except method evaluation was set to TRUE The top ten unique non-overlapping binding poses were kept for the subsequent molecular dynamics simulations and 10 ligands systems each were used for the simulations The protein-ligand systems along with the TIP3P water model and a neutralizing salt concentration of 0.15 M NaCl were energy minimized using 5000 steps with an energy tolerance of 1000 KJ/mol/nm The systems were subsequently equilibrated in 1 ns NVT and 4 ns NPT steps with a 1 fs timestep The constant temperature for all runs was 310 K and the Berendsen pressure coupling was used Production steps were then run for 100 ns with a 2 fs timestep with the Parrinello–Rahman barostat The residues and ligand atoms within 6 Å were selected for the calculation were averaged for 1000 frames and plotted for each simulation The results of the three 1 ligand systems were averaged All visualizations were made using the visual molecular dynamics (VMD) software113 data were normalized by total protein intensity in each technical replicate Then all the abundances were transformed into log10 and NA values were imputed by a constant value of −10 (in the heatmap figure) all samples were analyzed with three technical replicates there were four individual samples per group with no technical replicates Statistical t-test with unequal variance were used to compare the differences between groups Data analysis was performed using R (R version 4.1.0) with the help of ggplot2 All measurements were performed as a triplicate analysis of a given aliquot The initial DIA analysis was performed in one replicate The experiments on different NPs with PtdChos and DIA were performed on plasma samples from four individual donors Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article The human plasma proteome draft of 2017: building on the human plasma PeptideAtlas from mass spectrometry and complementary assays The challenge of the proteome dynamic range and its implications for in‐depth proteomics Mining the plasma proteome for disease applications across seven logs of protein abundance In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta deep and precise profiling of the plasma proteome with multi-nanoparticle protein corona Single chain variable fragment displaying M13 phage library functionalized magnetic microsphere-based protein equalizer for human serum protein analysis Digestion and depletion of abundant proteins improves proteomic coverage Plasma proteome profiling to detect and avoid sample‐related biases in biomarker studies Mass spectrometry-based plasma proteomics: considerations from sample collection to achieving translational data Unbiased identification of the liposome protein corona using photoaffinity-based chemoproteomics ProTargetMiner as a proteome signature library of anticancer molecules for functional discovery A streamlined high-throughput plasma proteomics platform for clinical proteomics with improved proteome coverage high-throughput depletion strategy for deep plasma proteomics Affinity capture enrichment versus affinity depletion: a comparison of strategies for increasing coverage of low-abundant human plasma proteins Abundant plasma protein depletion using ammonium sulfate precipitation and protein A affinity chromatography Depletion of abundant plasma proteins and limitations of plasma proteomics A novel scavenging tool for cancer biomarker discovery based on the blood-circulating nanoparticle protein corona Protein corona fingerprinting to differentiate sepsis from non-infectious systemic inflammation Nanotools for sepsis diagnosis and treatment Physical–chemical aspects of protein corona: relevance to in vitro and in vivo biological impacts of nanoparticles A highly efficient protein corona-based proteomic analysis strategy for the discovery of pharmacodynamic biomarkers Disease-specific protein corona sensor arrays may have disease detection capacity Rapid plasma proteome profiling via nanoparticle protein corona and direct infusion mass spectrometry Proteomic analysis reveals distinct protein corona compositions of citrate- and riboflavin-coated SPIONs How eluents define proteomic fingerprinting of protein corona on nanoparticles The protein corona from nanomedicine to environmental science An overview of nanoparticle protein corona literature The issue of reliability and repeatability of analytical measurement in industrial and academic nanomedicine Cholesterol modulates the physiological response to nanoparticles by changing the composition of protein corona Effects of cholesterol on biomolecular corona Vitamin B6 metabolism and binding to proteins in the blood of alcoholic and nonalcoholic men Simultaneous binding of folic acid and methotrexate to human serum albumin: insights into the structural changes of protein and the location and competitive displacement of drugs Molecular recognition patterns between vitamin B12 and proteins explored through STD-NMR and in silico studies and expression of the pantothenate permease (panF) gene of Escherichia coli The tuberculosis structural genomics consortium: a structural genomics approachto drug discovery In: Advances in Protein Chemistry and Structural Biology (ed Interaction between carbon dots from folic acid and their cellular receptor: a qualitative physicochemical approach Molecular mechanisms of the non-coenzyme action of thiamin in brain: biochemical Structural analysis and insight into effector binding of the niacin-responsive repressor NiaR from Bacillus halodurans Variation of protein corona composition of gold nanoparticles following plasmonic heating Regulation of macrophage recognition through the interplay of nanoparticle surface functionality and protein corona Protein corona composition of gold nanocatalysts Optical sensor arrays for chemical sensing: the optoelectronic nose Irreversible changes in protein conformation due to interaction with superparamagnetic iron oxide nanoparticles Protein corona composition of superparamagnetic iron oxide nanoparticles with various physico–chemical properties and coatings Graphene oxide strongly inhibits amyloid beta fibrillation Hard corona composition and cellular toxicities of the graphene sheets Personalized disease-specific protein corona influences the therapeutic impact of graphene oxide Protein-Nanoparticle Interactions: the Bio-nano Interface (Springer Science & Business Media Zeolite nanoparticles for selective sorption of plasma proteins Corona protein composition and cytotoxicity evaluation of ultra-small zeolites synthesized from template free precursor suspensions Personalized protein coronas: a “key” factor at the nanobiointerface Sex-specific silica nanoparticle protein corona compositions exposed to male and female BALB/c mice plasmas Nanoscale characterization of the biomolecular corona by cryo-electron microscopy Measurements of heterogeneity in proteomics analysis of the nanoparticle protein corona across core facilities A uniform data processing pipeline enables harmonized nanoparticle protein corona analysis across proteomics core facilities Bacterial effects and protein corona evaluations: crucial ignored factors in the prediction of bio-efficacy of various forms of silver nanoparticles Exploring cellular interactions of liposomes using protein corona fingerprints and physicochemical properties Nanoparticles-cell association predicted by protein corona fingerprints Differential uptake of functionalized polystyrene nanoparticles by human macrophages and a monocytic cell line Nanoparticle size is a critical physicochemical determinant of the human blood plasma corona: a comprehensive quantitative proteomic analysis Pre-adsorption of antibodies enables targeting of nanocarriers despite a biomolecular corona Biomolecular coronas provide the biological identity of nanosized materials Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology The need for improved methodology in protein corona analysis Disease-related metabolites affect protein–nanoparticle interactions Interactions of triglycerides with phospholipids: incorporation into the bilayer structure and formation of emulsions Interactions of phosphatidylcholine surface monolayers with triglyceride cores and enhanced ApoA-1 binding in lipid emulsions Bottom-up and top-down proteomic approaches for the identification and quantification of the low molecular weight proteome with focus on short open reading frame-encoded peptides Selected reaction monitoring-based proteomics: workflows Proteomics by mass spectrometry: approaches Standardizing protein corona characterization in nanomedicine: a multicenter study to enhance reproducibility and data homogeneity Apolipoprotein E: from cardiovascular disease to neurodegenerative disorders Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts Effects of the presence or absence of a protein corona on silica nanoparticle uptake and impact on cells Plasma concentration gradient influences the protein corona decoration on nanoparticles The protein corona mediates the impact of nanomaterials and slows amyloid beta fibrillation Proteoforms as the next proteomics currency Deep top-down proteomics revealed significant proteoform-level differences between metastatic and nonmetastatic colorectal cancer cells Distinct hypertrophic cardiomyopathy genotypes result in convergent sarcomeric proteoform profiles revealed by top-down proteomics Mass spectrometry-based top-down proteomics in nanomedicine: proteoform-specific measurement of protein corona Understanding biophysicochemical interactions at the nano–bio interface The crystal structure of human microsomal triglyceride transfer protein Interaction of C-reactive protein with artificial phosphatidylcholine bilayers Phosphatidylserine and phosphatidylethanolamine bind to protein Z cooperatively and with equal affinity Effect of phosphorylation of phosphatidylinositol on myelin basic protein-mediated binding of actin filaments to lipid bilayers in vitro Structure of human phosphatidylcholine transfer protein in complex with its ligand Interaction of phosphatidylcholine with bovine serum albumin Specificity and properties of the complexes Leakage of sucrose from phosphatidylcholine liposomes induced by interaction with serum albumin Lipid–protein interactions in the plasma lipoproteins Mapping the KRAS proteoform landscape in colorectal cancer identifies truncated KRAS4B that decreases MAPK signaling Progress in Top-down proteomics and the analysis of proteoforms Rafieioskouei, A., Rogale, K., Dibavar, A. S., Mahmoudi, M. & Bonakdarpour, B. Causality analysis of protein corona composition: phosphatidylcholine-enhances plasma proteome profiling by proteomics. Preprint at bioRxiv https://doi.org/10.1101/2024.09.10.612356 (2024) IgY14 and SuperMix immunoaffinity separations coupled with liquid chromatography-mass spectrometry for human plasma proteomics biomarker discovery Deciphering nanoparticle protein coronas by capillary isoelectric focusing-mass spectrometry-based top-down proteomics Estimating evolutionary rates using time-structured data: a general comparison of phylogenetic methods TopFD: a proteoform feature detection tool for top-down proteomics Large-scale qualitative and quantitative top-down proteomics using capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry with nanograms of proteome samples Update of the CHARMM all-atom additive force field for lipids: validation on six lipid types AutoDock Vina: improving the speed and accuracy of docking with a new scoring function Crystallographic analysis reveals common modes of binding of medium and long-chain fatty acids to human serum albumin GROMACS 4.5: a high-throughput and highly parallel open source molecular simulation toolkit Optimization of the additive CHARMM all-atom protein force field targeting improved sampling of the backbone ϕ ψ and side-chain χ1 and χ2 dihedral angles gmx_MMPBSA: a new tool to perform end-state free energy calculations with GROMACS MMPBSA.py: an efficient program for end-state free energy calculations MD-ligand-receptor: a high-performance computing tool for characterizing ligand-receptor binding interactions in molecular dynamics trajectories The PRIDE database and related tools and resources in 2019: improving support for quantification data Download references gratefully acknowledges financial support from the U.S National Institute of Diabetes and Digestive and Kidney Diseases (grant DK131417) was supported by an Ambizione Fellowship from the Swiss National Science Foundation (SNSF grant number: PZ00P3_216203) and a grant from Karolinska Institutet (2-188/2022) thanks the support from the National Cancer Institute (NCI) through the grant R01CA247863 We acknowledge the support of a Burroughs Wellcome Fund Career Award at the Scientific Interface (CASI) (M.P.L.) and a polymaths award from Schmidt Sciences is a Chan Zuckerberg Biohub San Francisco investigator Open access funding provided by Karolinska Institute These authors contributed equally: Ali Akbar Ashkarran Ali Akbar Ashkarran & Morteza Mahmoudi Department of Medical Biochemistry and Biophysics Department of Chemical and Biomolecular Engineering Departments of Bioengineering and Mechanical Engineering University of Tennessee Health Science Center Medical College of Georgia at Augusta University and hypothesis development were done by (A.A.S performed experimental procedures on the formation and analysis of protein corona; and sent the samples for bottom-up-proteomics analysis and different core facilities conducted and supervised (respectively) the protein corona analysis using top-down-proteomics conducted and discussed molecular dynamics analysis conducted the bottom-up proteomics of the samples Morteza Mahmoudi discloses that (i) he is a co-founder and director of the Academic Parity Movement (www.paritymovement.org) a non-profit organization dedicated to addressing academic discrimination and incivility; (ii) he is a co-founder of Targets’ Tip and Albuderm; and (iii) he receives royalties/honoraria for his published books The remaining authors declare no competing interests Nature Communications thanks Amitava Mukherjee and the other reviewers for their contribution to the peer review of this work Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Download citation DOI: https://doi.org/10.1038/s41467-024-53966-z Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma. Print El Sobrante Landfill managers informed air quality regulators last year that a chemical reaction brewing underground was causing broiling temperatures and toxic sulfur pollution.Waste Management said the chemical reaction hasn’t interfered with daily operations and is about 3,000 feet from the section of the landfill that would receive disaster debris state regulators say the landfill will no longer accept fire debris The Eaton and Palisades fires had barely broken out when Waste Management offered to accept the inevitable disaster debris at its El Sobrante Landfill near Corona 8 for an emergency waiver to accept the wildfire rubble landfill staffers had been struggling for months to control a fiery situation of their own El Sobrante managers informed the South Coast Air Quality Management District that a chemical reaction brewing inside the landfill was causing broiling temperatures and producing toxic sulfur pollution Waste Management sought approval to increase its tonnage capacity to take in wildfire debris anyway which Riverside County officials granted the next day Riverside County officials are now requesting help from state officials for technical assistance with the situation They acknowledge that they were notified by environmental regulators about the elevated temperatures but declined to address whether granting the waiver was appropriate So far, no fire debris has been taken to the landfill, according to the U.S. Army Corps of Engineers, the federal agency overseeing the debris cleanup. But, as of Thursday morning, it had remained on a list of sites that were eligible to accept this material Since The Times inquired about the chemical reaction at El Sobrante state environmental regulators say they have ruled out allowing fire debris to be taken there “Debris from this year’s fires has not been sent to the El Sobrante landfill nor is the site under consideration for future use for this purpose,” said Nefretiri Cooley a spokesperson for the California Environmental Protection Agency “We are currently investigating this incident and will provide additional details as they are available.” Waste Management executives say the chemical reaction has not interfered with daily operations at the 1,322-acre landfill is about 3,000 feet from the section of the landfill where they had planned to dump disaster debris were irked that the landfill had even sought permission “I’m shocked and appalled that El Sobrante would take ash when it knows that its landfill is on fire,” said Jane Williams executive director of California Communities Against Toxics “You greatly increase the public health danger to the communities surrounding when landfills are being operated like this An out-of-control landfill can emit more [toxic] benzene than an oil refinery.” The situation at El Sobrante Landfill underscores the checkered compliance history at many Southern California landfills that are poised to receive an estimated 4.5 million tons of potentially hazardous ash rubble and soil from the federal cleanup efforts With such problems as excessive air pollution and insufficient inspections state and local environmental regulators have handed down dozens of violations to these waste disposal facilities in the last year alone El Sobrante is the second landfill in Southern California in recent years to experience this type of scorching-hot chemical reaction — commonly called an underground landfill fire. Since 2022, operators of the since-closed Chiquita Canyon Landfill in Castaic struggled to contain noxious fumes and contaminated runoff caused by an underground garbage fire These underground dump fires cannot be easily extinguished and often take years to peter out these incidents have called into question the environmental oversight of California’s landfills The California Department of Resources Recycling and Recovery El Sobrante staff members told the local air district that the landfill had experienced a rise in highly flammable hydrogen gas and lung-aggravating dimethyl sulfide emissions — both telltale signs of abnormal temperatures inside the landfill Waste Management executives said in August that the pollution was emanating from a two-acre “area of concern” where landfill staff had observed temperatures climbing above 200 degrees Staff members also reported cracks on the landfill’s surface landfill workers began extracting liquid waste from the reaction area to alleviate the heat and pressure transporting it to a waste treatment facility “South Coast AQMD continues to conduct unannounced on-site inspections at the landfill and responds to all public complaints,” said Nahal Mogharabi The conditions at El Sobrante Landfill mirror issues at Chiquita Canyon Landfill where residents called in thousands of odor complaints to the local air district Air district inspectors found white smoke venting from cracks on the landfill’s surface and liquid waste bursting onto the surface in a geyser-like fashion. The Environmental Protection Agency said the reaction posed an “imminent” danger to public health and the environment County officials eventually helped organize a temporary relocation program for residents who wanted to escape the stench and toxic fumes Waste Connections, the owner of Chiquita Canyon, closed the landfill in December and is focusing on managing the reaction area Federal and state environmental regulators suspected the Chiquita Canyon reaction was caused by oxygen intrusion landfills have an extensive network of underground wells that are used to extract gases produced as buried waste decomposes But if this system fails or overdraws these gases these wells can introduce oxygen into the waste speeding up waste decomposition and producing heat the extreme heat has the potential to damage the protective liner underneath the landfill that prevents liquid waste from seeping into the groundwater beneath and migrating to neighboring areas 2:36 p.m. Feb. 28, 2025A previous version of the article stated that CalRecycle inspectors identified several violations at El Sobrante Landfill. Riverside County inspectors, who work in partnership with CalRecycle, found those violations. In the last year, El Sobrante Landfill has been cited several times for excessive air pollution by the local air district. In that time, Riverside County inspectors also identified violations for elevated levels of flammable methane, improper signage and failure to conduct sufficient truck inspections to ensure hazardous wastes aren’t disposed at the site. Tony Briscoe is an environmental reporter with the Los Angeles Times. His coverage focuses on the intersection of air quality and environmental health. Prior to joining The Times, Briscoe was an investigative reporter for ProPublica in Chicago and an environmental beat reporter at the Chicago Tribune. World & Nation Climate & Environment and yachting from her home on the Côte d’Azur where she has lived for nearly two decades Her stories have appeared in Condé Nast Traveler She is also a contributing author to four Lonely Planet guidebooks TechThe Famous Group Corona Extra team on MLB Opening Day mixed-realty activationThe 30-second spot appeared during the fourth inning of Dodgers-Tigers on ESPN ESPN’s Opening Day broadcast of Dodgers-Tigers included a unique mixed-reality activation developed by The Famous Group in partnership with Corona Extra and Initiative the beer brand’s paid media agency of record introduced the 30-second spot by declaring “Playa is always just a lime away,” in line with Corona’s popular tagline Dodger Stadium was then transformed into an animated beachfront by The Famous Group’s new “Virtual Camera” mixed-reality system centering on two beach chairs and a bucket of beers four cameras were positioned around Dodger Stadium This is the second mixed-reality spot TFG has executed at Dodger Stadium, joining a Denzel Washington-narrated Jackie Robinson tribute ahead of the 2022 All-Star Game (using its old system) TFG’s mixed-reality initiatives are led by the company’s Creative Dir Hemu Karadkar and VP/Production Technology Erik Beaumont Metrics details Polymeric nanoparticles (NPs) are promising tools used for immunomodulation and drug delivery in various disease contexts The interaction between NP surfaces and plasma-resident biomolecules results in the formation of a biomolecular corona which varies patient-to-patient and as a function of disease state This study investigates how the progression of acute systemic inflammatory disease influences NP corona compositions and the corresponding effects on innate immune cell interactions NP coronas alter cell associations in a disease-dependent manner induce differential co-stimulatory and co-inhibitory molecule expression Integrated multi-omics analysis of proteomics and cytokine datasets highlight a set of differentially enriched TLR4 ligands that correlate with dynamic NP corona-mediated immune activation Pharmacological inhibition and genetic knockout studies validate that NP coronas mediate this response through TLR4/MyD88/NF-κB signaling Our findings illuminate the personalized nature of corona formation under a dynamic inflammatory condition and its impact on NP-mediated immune activation profiles and inflammation suggesting that disease progression-related alterations in plasma composition can manifest in the corona to cause unintended toxicity and altered therapeutic efficacy a Schematic representation of the endotoxemia mouse model and plasma extractions for NP corona formation. Plasmas were extracted after varying time-points to assess different inflammation severities (n = 10 mice per timepoint) and used to coat NPs for subsequent macrophage treatments. Created in BioRender. Shaw, J. (2025) https://BioRender.com/s80i128 b Pro-inflammatory cytokine profiles of pooled whole mouse plasma after 0 and 16 h-post LPS injection (representing 3 measurements of n = 10 pooled mouse plasmas per timepoint) c Total protein quantified in whole plasma over time using a bicinchoninic acid (BCA) protein assay (n = 3 biological replicates) d Nanoparticle Tracking Analysis (NTA) of NP corona diameter size distributions after washing off excess plasma Plots are representative of n = 3 measurements of 30 second recordings Error bars are represented as red-shaded regions Mean size and zeta-potential are presented for each condition and 8hrPlas samples were eluted and run through an SDS-PAGE gel and stained with Coomassie for total protein f Quantification of proteins eluted from NP coronas using a 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) protein assay represented as ng of protein per mg NP (n = 3 independent replicates) Significance was calculated using a one-way ANOVA with Tukey post hoc test g Uptake/association kinetic analysis of Cy5.5-labeled PLGA NPs or NP corona-treated macrophages up to 24 h quantified using flow cytometry (n = 3 biological replicates) Significance was determined in the final 24-hour timepoint using a one-way ANOVA with Tukey’s post-hoc test (PLGA:NaivePlas_PLGA *P < 0.05; **P < 0.01; ***P < 0.001 Source data are provided as a Source Data file a Flow cytometry mean fluorescence intensity (MFI) quantification of macrophage surface markers CD86 and PD-L1 after treatment with pristine PLGA NPs or NP coronas for 30-min or 24-h (n = 3 biological replicates per timepoint) Representative histograms of 24-hour timepoints are shown below Statistical significance was determined with a two-way ANOVA using a Dunnett’s test with No Treatment set as control b TNFα secretions over time from macrophages after PLGA NP or NP corona treatment (n = 3 biological replicates per timepoint) c Multiplex cytokine analysis in the supernatants of NP corona-treated macrophages after 3-hour incubation (n = 3 biological replicates) Statistical significance was determined with a two-way ANOVA using Tukey post hoc test d Spearman correlation matrix of the macrophage multiplex cytokine analysis indicating a strong positive correlation between sample cytokines e Organ biodistribution of Cy5.5-labeled PLGA NPs coated in various plasma coronas 3-h post i.v and brain were isolated and analyzed via in vivo imaging system (IVIS) for NP fluorescence Representative organ samples are presented from saline (S) Percent of total radiant efficiency is presented in the graph below (n = 2 mice per group) No fluorescence was detected in brain samples f TNFα plasma concentration from NP corona-treated IVIS mice (n = 2 mice per group Significance was calculated using a two-way ANOVA with Tukey post hoc test The difference for 8hrPlas compared to the in vitro findings can be attributed to 8hrPlas NP coronas likely stimulating other immune cells in vivo these findings demonstrate the differential immunostimulatory capabilities of various NP coronas caused by NP composition and disease state dynamics suggesting that the differential presence of biomolecules in coronas are contributing to the measured alterations in immunological profiles k Upstream network effects that were supervised to predict 3hrPlas corona-induced cytokines Color key and symbols are reported in the legend although the host plasma is laden with pro-inflammatory molecules at the 8hrPlas timepoint their lack of cellular association could reduce the induction of pro-inflammatory cellular responses when compared to 3hrPlas_PLGA samples these results show that there is likely a compilation of biomolecules either specific to or significantly elevated in the 3hrPlas NP coronas that have the propensity to induce inflammatory cytokines and while 8hrPlas NP coronas also contain similar pro-inflammatory biomolecules the marked reduction in opsonins may reduce their potency Significance for all graphs were calculated using a one-way ANOVA with Tukey’s post-hoc test Although it is possible that LPS may contribute to the inflammatory response our data supported that it is unlikely to be the driving biomolecule in the 3hrPlas NP coronas a Upstream network prediction further restricted to 3hrPlas-specific corona biomolecules that induce TLR4/MyD88/NF-kB activation. b Schematic representation of recombinant protein pre-coatings to evaluate potential pro-inflammatory biomolecules. Created in BioRender. Shaw, J. (2025) https://BioRender.com/u64r124 c Pro-inflammatory TNFα secretion of macrophages treated with various pre-coated NP coronas NP coronas evaluated include hemoglobin (20 mg/mL) coatings (HbHigh_PLGA) fibrinogen normal (2 mg/mL) and high (10 mg/mL) (FbNorm_PLGA 3hrPlas_PLGA spiked with Paquinimod S100A9 inhibitor along with healthy (Naïve) plasma coatings spiked with hemoglobin or fibrinogen (Measurements are from n = 3 biological replicates for all samples Significance was calculated using a one-way ANOVA with Tukey’s post-hoc test *P < 0.05; **P < 0.01; ***P <  0.001 although there is previous literature demonstrating disease-specific protein corona composition differences in human plasmas evaluating the effects of disease dynamics on corona-dependent immune recognition in additional disease models could help to fine-tune the administration of NPs by identifying an optimal therapeutic window where coronas do not induce immune activation or possibly limit therapeutic efficacy it is likely that many patients will be medicated with other therapeutic agents that could affect the composition of the NP corona and corresponding responses this work demonstrates that NP coronas are formed differentially due to disease progression which significantly affects corona formation and corresponding immune activation profiles These findings contribute to the nanomedicine field by providing evidence that disease-relevant NP coronas should be assessed on a formulation-specific basis to enhance preclinical testing and potential clinical translation previously unsuccessful NP formulations may benefit from re-evaluation with more precise classifications of patient subpopulations and disease-relevant corona characterization Acid-terminated 50:50 PLGA (∼0.17 dL/g inherent viscosity in hexafluoro-2-propanol; approximately MW 4.2 kDa) and acid-terminated poly(DL-lactide) (PLA) (approximately MW 11.3 kDa) with low inherent viscosity between 0.16–0.25 dL/g (Product No B6014-1) were purchased from Lactel Absorbable Polymers (Birmingham Poly(ethylene-alt-maleic anhydride) E400 (PEMA 105564) was received as a gift from Vertellus™ (Indianapolis Poly(ethylene glycol) methyl ether-block-poly(lactide-co-glycolide) (PLGA-PEG5k) (Product No LPS from Escherichia Coli serotype O111:B4 FITC-conjugated LPS from Escherichia coli serotype O111:B4 and acetone were obtained from Sigma-Aldrich (St HY-139397) was purchased from MedChemExpress (Monmouth Junction Endotoxin quantification assay (Product No C6667) were purchased from Thermo Fisher Scientific (Waltham RAW-Blue™ cells and QUANTI-Blue™ solution (Cat raw-sp) were purchased from InvivoGen (San Diego Female C57BL/6 mice (5–6 weeks old) were purchased from the University of Maryland Vet Resources (Baltimore TLR4-/- K/O C57BL/6 J (B6(Cg)-Tlr4tm1.2Karp/J) mice were gifted by S Mice were housed on a 12-hour light/12-hour dark cycle at ambient temperature (65–75oC) and n-propanol were purchased from Fisher Scientific (Pittsburg and formic acid was purchased from Sigma Aldrich (St EquiSPLASH lipidomix was purchased from Avanti Polar Lipids Flow rate ratio of 1 was used during NP synthesis to achieve consistent size distributions PLGA and PLA NPs were then subjected to centrifugation at 13,000xg while PLGA-PEG5k NPs were centrifuged at 50,000xg to eliminate excess surfactant by washing thrice with cold H2O Sucrose (4% w/v) and mannitol (3% w/v) were added to the NP suspensions as a cryoprotectant The NPs were then frozen at -80 °C and lyophilized for later storage at RT in a desiccator The size and zeta potential of PLGA NPs and coronas were determined in 1x PBS by dynamic light scattering (DLS) using a Malvern Zetasizer Nano ZSP and Nanoparticle Tracking Analysis (NTA) using a Malvern Nanosight NS300 (Malvern UK) set to n = 3 recording measurements at a flow rate of 20 µL/minute Final NP corona pellets were resuspended in 1x PBS for use in subsequent experiments Plasma was collected into K2 EDTA coated tubes and pooled at 3 and 16-h-post LPS injection for plasma analysis and subsequent ex-vivo NP corona formation Mice were housed under specific pathogen-free conditions in a facility at the University of Maryland All mice experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of the University of Maryland NP corona organ distribution: Cy5.5-labeled PLGA NP coronas were injected intravenously through the tail vein at 2 mg per mouse Mice were then sacrificed after 3 h; organs and plasmas were isolated for Xenogen in vivo imaging system (IVIS) analysis of organ distribution and ELISA plasma cytokine quantification Total radiant efficiency was calculated for all organs on a per-mouse basis and used to calculate the percent of NP amount in each organ To determine the concentration of total plasma protein a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Plasma samples were diluted 1000-fold before protein quantification following manufacturers protocol The protein concentration was determined by comparing the absorbance at 562 nm to a standard curve prepared using bovine serum albumin (BSA) using a SpectraMax iD3 Microplate Reader (Molecular Devices PLGA NPs were incubated in various plasmas to form coronas as described above NP coronas were then dissolved in DMSO at a concentration of 20 mg/mL to solubilize the polymer and adsorbed proteins 10 µL of dissolved NP corona samples were mixed with 10 µL of 5 mM ATTO-TAG CBQCA reagent and 125 µL of 0.1 M sodium borate buffer (pH 9.3) and measured for fluorescence at an excitation of 465 nm and emission at 550 nm Quantification of endotoxin was accomplished by a 1:1000 dilution of whole plasma or NP corona samples following the manufacturer’s protocol 0.5 mgs of NPs per condition were mixed with Laemmli buffer (0.277 M Tris-HCl 0.02% bromophenol blue) and heated to 95 °C for 5 min Samples were then centrifuged at 13,000 xg to separate denatured proteins from solid NPs and supernatants containing eluted corona proteins were run through a 4%-15% gradient SDS/PAGE gel A molecular weight protein standard (Product No gels were stained for total protein with Coomassie for 2 h and destained in a solution of 50:40:10 water:methanol:acetic acid Images were taken using a ThermoFisher iBright imaging system and processed on ImageJ (Version 1.54 h) Protein identification was filtered to achieve a 1% false discovery rate (FDR) in peptide spectra match (PSM) This FDR was determined using the Percolator algorithm embedded in PD The resulting protein abundances were analyzed using Perseus software (version 1.6.14.0) data was further filtered to include only proteins identified without any missing values in all the biological replicates The quantitative protein data were log2 transformed and normalized using median centering Differentially expressed proteins from various conditions were identified using ANOVA-based analysis or Two-tailed student’s t-test (adjusted p-value < 0.05) a mixture of 400 µL cold methanol and 10 µL internal standard (EquiSPLASH lipidomix) was added to each sample followed by orbital shaking at 4 °C for 15 min and the samples were incubated at 4 °C for 1 hour with orbital shaking and the resulting extract was incubated at 4 °C for 15 min with orbital shaking Samples were then centrifuged at 6010x g for 8 min at 4 °C to allow for phase separation The upper organic phase was carefully collected and kept on ice while the lower aqueous phase was re-extracted with 200 µL of MTBE followed by 15 min of incubation at 4 °C with orbital shaking After an additional centrifugation at 6010x g for 8 min at 4 °C the upper organic phase was removed and combined with the first organic extract Organic extracts were then dried under a steady stream of nitrogen at 30 °C to remove solvents and the recovered lipids were dissolved in 200 µL of chloroform:methanol (1:1 v/v) containing 200 µM of butylated hydroxytoluene samples were diluted 4 fold with acetonitrile:isopropanol:water (1:2:1 The lower aqueous phase was used to determine the protein content via a BCA kit (bicinchoninic acid assay Liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS) on an Agilent 1290 Infinity LC coupled to an Agilent 6560 Quadrupole Time-of-Flight (Q-TOF) mass spectrometer was used to analyze total lipid extracts The separation was carried out using a C18 CSH (1.7 µm; 2.1 ×100 mm) column (Waters Mobile phase A was 10 mM ammonium formate with 0.1% formic acid in water/acetonitrile (40:60 v/v) and mobile phase B was 10 mM ammonium formate with 0.1% formic acid in acetonitrile/isopropanol (10:90 The gradient was ramped from 40% to 43% B in 1 min the gradient was returned to initial conditions in 0.5 min and held constant for 1.6 min at a flow rate of 0.4 mL/min The column was heated to a temperature of 55 °C and the auto-sampler was kept at 5 °C during the analysis A 2 µL injection volume was used for all samples analyses Two workflows were used for mass spectrometry analysis lipid identification of a pooled sample using an iterative MS/MS acquisition lipid semi-quantitation of all samples using high-resolution The MS parameters for the iterative workflow were employed as described: extended dynamic range m/z 980.0164 (–); MS and MS/MS Range m/z 100–1700; acquisition rate narrow ( ~ 1.3 m/z); collision energy 20 eV (+) 5,000 counts and 0.001%; active exclusion enabled yes; purity common organic molecules; static exclusion ranges The MS parameters for the MS1 workflow were the same for source and reference mass parameters and differed only for acquisition (selection of MS (same parameters) not Auto MS/MS) Univariate analysis performed using Prism 6 (GraphPad Isolated bone marrow was then subjected to ACK lysis and residual stem cells were cultured in complete RPMI 1640 media containing penicillin (100 units/mL) 10% heat-inactivated fetal bovine serum (VWR and 20% L929 cell conditioned medium (containing M-CSF) to induce BMDM differentiation BMDMs were allowed to differentiate for 8 days with cell conditioned media changes on days 3 and 6 BMDMs from Naïve mice or TLR4 K/O mice (1 × 105 cells/well) were seeded in a sterile 24-well plate and treated with 300 μg/mL of PLGA NPs or the different NP coronas at 37 °C and 5% CO2 Supernatants were taken after 3 h of incubation Cytokine profiles in cell culture supernatants and diluted mouse plasmas were analyzed using an enzyme-linked immunosorbent assay (ELISA) (BioLegend CA) for TNFα or a custom 7-plex Luminex panel for IL-1β and IFN-β following the manufacturer’s protocols and analyzed using the Luminex xPONENT software (Thermo Fisher Heatmap and PCA plots were generated using MetaboAnalyst 5.0 BAY 11-7082 was used to inhibit NF-κB where cells were pre-treated at 10 μM for 30 min prior to NP corona treatment for 3 h TJ-2010-5 was used to inhibit MyD88 activation where cells were pre-treated at 30 µM for 2 h prior to NP corona treatment RAW-Blue cells were cultured in DMEM (4.5 g/L glucose Pen-Strep (100 U/mL) following the manufacturer protocol Cells were treated with 300 µg/mL PLGA NPs or NP coronas Supernatants from cells were collected 24-h post-stimulation 20 μL of supernatant was mixed with 180 μL of QUANTI-Blue solution and allowed to react in the dark at 37 °C for two hours Secreted alkaline phosphatase (SEAP) activity was measured by reading the absorbance at 620 nm using a microplate reader Day 8 BMDMs (1 × 105 cells/well) were seeded in a sterile 24-well plate and treated with 300 μg/mL of Cyanine-5.5 labeled PLGA NP samples After NP corona treatment for 30 min or 24 h cells were washed with fresh media and lifted with Versene solution Lifted cells were incubated with anti‐CD16/32 (1:500 Clone 93) antibody to block nonspecific binding before staining with anti-mouse CD11b-Pacific Blue (1:500 Clone 10 F.9G2) antibodies from BioLegend (San Diego Cell viability was measured with propidium Iodide (PI) from BioLegend The stained cells were measured on a Cytek Aurora flow cytometer (Fremont CA) and analyzed using FCS Express 7 De Novo Software (Glendale P-values were corrected using a two-way ANOVA with a Benjamini-Hochberg algorithm FDR correction The list of differentially abundant biomolecules (proteins and metabolites) between 3hrPlas_PLGA and NaivePlas_PLGA coronas were uploaded into the IPA software (Qiagen) The “core analysis” function was used to interpret the datasets Causal network analysis was supervised based on the observed BMDM cytokine profiles in order to predict 3hrPlas corona-mediated pathway activation Further TLR4 ligand prediction was done by using the pathway “grow” feature to identify direct binders from the 3hrPlas_PLGA NP coronas Purified hemoglobin at 10 mg/mL and fibrinogen (Fb) at 2 mg/mL (FbNorm) or 10 mg/mL (FbHigh) were incubated with PLGA NPs at 37 °C for 1 hour purified hemoglobin or fibrinogen was spiked into Naïve plasma at a final concentration of 10 mg/mL then incubated with NPs to attempt to simulate 3hrPlas concentrations All NPs were then washed thrice with cold 1x PBS as described earlier and BMDM cells were treated with 300 µg/mL for 3 h before supernatant TNFα cytokine analysis Paquinimod was added into whole 3hr-post LPS plasma at a final concentration of 30 µM Samples were allowed to incubate for 1 hour to allow for inhibitor binding Inhibited 3 hr plasma was then used to coat PLGA NPs as stated above Washed NP coronas were then used to treat BMDM cells at 300 µg/mL for 3 h before supernatant cytokine analysis The data analysis was performed using GraphPad Prism 10.4.1 (GraphPad Software Ins.) Results are presented as mean ± standard deviation of n = 3 independent experiments unless stated otherwise Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article On the issue of transparency and reproducibility in nanomedicine Nanoparticle Size Is a Critical Physicochemical Determinant of the Human Blood Plasma Corona: A Comprehensive Quantitative Proteomic Analysis Understanding the nanoparticle–protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles Binding of blood proteins to carbon nanotubes reduces cytotoxicity Interplay of protein corona and immune cells controls blood residency of liposomes Nanoparticle-induced unfolding of fibrinogen promotes Mac-1 receptor activation and inflammation Formation of the Protein Corona: The Interface between Nanoparticles and the Immune System Ju, Y. et al. Person-Specific Biomolecular Coronas Modulate Nanoparticle Interactions with Immune Cells in Human Blood. ACS Nano https://doi.org/10.1021/acsnano.0c06679 (2020) Personalized liposome–protein corona in the blood of breast Personalized protein corona on nanoparticles and its clinical implications Triad of Acute Infusion-Related Reactions Associated with Liposomal Amphotericin B: Analysis of Clinical and Epidemiological Characteristics Can the biomolecular corona induce an allergic reaction?—A proof-of-concept study Roadmap and strategy for overcoming infusion reactions to nanomedicines Applying lessons learned from nanomedicines to understand rare hypersensitivity reactions to mRNA-based SARS-CoV-2 vaccines Nanoparticle-Binding Immunoglobulins Predict Variable Complement Responses in Healthy and Diseased Cohorts Precision Nanomedicine Development Based on Specific Opsonization of Human Cancer Patient-Personalized Protein Coronas Nanoparticle-Enabled Enrichment of Longitudinal Blood Proteomic Fingerprints in Alzheimer’s Disease Gold Nanoparticle-Enabled Blood Test for Early Stage Cancer Detection and Risk Assessment Analysis of the Human Plasma Proteome Using Multi-Nanoparticle Protein Corona for Detection of Alzheimer’s Disease An investigation on the role of plasma and serum opsonins on the evternalization of biodegradable poly(D,L-lactic acid) nanoparticles by human monocytes Time-dependent changes in opsonin amount associated on nanoparticles alter their hepatic uptake characteristics and pharmacokinetics of polymeric nanoparticles Disease-specific protein corona formed in pathological intestine enhances the oral absorption of nanoparticles PLGA-Based Nanomedicine: History of Advancement and Development in Clinical Applications of Multiple Diseases Development of Therapeutic Polymeric Nanoparticles for the Resolution of Inflammation Polymeric synthetic nanoparticles for the induction of antigen-specific immunological tolerance Tolerogenic Immune Modifying Nanoparticles Encapsulating Multiple Recombinant Pancreatic β Cell Proteins Prevent Onset and Progression of Type 1 Diabetes in NOD Mice PLGA-based nanoparticles: an overview of biomedical applications TAK-101 Nanoparticles Induce Gluten-Specific Tolerance in Celiac Disease: A Randomized Microfluidic-Generated Immunomodulatory Nanoparticles and Formulation-Dependent Effects on Lipopolysaccharide-Induced Macrophage Inflammation Serum albumin as a risk factor for death in patients with prolonged sepsis: An observational study Plasma protein levels are markers of pulmonary vascular permeability and degree of lung injury in critically ill patients with or at risk for acute lung injury/acute respiratory distress syndrome Nanoparticle Uptake: The Phagocyte Problem Differential Roles of the Protein Corona in the Cellular Uptake of Nanoporous Polymer Particles by Monocyte and Macrophage Cell Lines Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells Corona Composition Can Affect the Mechanisms Cells Use to Internalize Nanoparticles Changes in target ability of nanoparticles due to protein corona composition and disease state Polymeric particle-based therapies for acute inflammatory diseases Biomaterial-Driven Immunomodulation: Cell Biology-Based Strategies to Mitigate Severe Inflammation and Sepsis In vivo reprogramming of immune cells: Technologies for induction of antigen-specific tolerance Cargo-less nanoparticles program innate immune cell responses to Toll-like receptor activation Immunomodulatory Nanoparticles Mitigate Macrophage Inflammation via Inhibition of PAMP Interactions and Lactate-Mediated Functional Reprogramming of NF-κB and p38 MAPK Therapeutic Inflammatory Monocyte Modulation Using Immune-Modifying Microparticles Effects of Particle Size and Surface Modification on Cellular Uptake and Biodistribution of Polymeric Nanoparticles for Drug Delivery On the mechanism of tissue-specific mRNA delivery by selective organ targeting nanoparticles Engineering precision nanoparticles for drug delivery Time Evolution of the Nanoparticle Protein Corona In situ analysis of nanoparticle soft corona and dynamic evolution IgG and fibrinogen driven nanoparticle aggregation Omo-Lamai, S. et al. Physicochemical Targeting of Lipid Nanoparticles to the Lungs Induces Clotting: Mechanisms and Solutions. bioRxiv 2023.07.21.550080 https://doi.org/10.1101/2023.07.21.550080 (2023) An Updated Review of Pro- and Anti-Inflammatory Properties of Plasma Lysophosphatidylcholines in the Vascular System Biological Features of Nanoparticles: Protein Corona Formation and Interaction with the Immune System Understanding Protein–Nanoparticle Interaction: A New Gateway to Disease Therapeutics The complement system testing in clinical laboratory Complement Depletion Deteriorates Clinical Outcomes of Severe Abdominal Sepsis: A Conspirator of Infection and Coagulopathy in Crime Complement activation in severely ill patients with sepsis: no relationship with inflammation and disease severity Complement proteins bind to nanoparticle protein corona and undergo dynamic exchange in vivo The anti-inflammatory drug BAY 11-7082 suppresses the MyD88-dependent signalling network by targeting the ubiquitin system Targeting of MyD88 Homodimerization by Novel Synthetic Inhibitor TJ-M2010-5 in Preventing Colitis-Associated Colorectal Cancer Identification of Lps2 as a key transducer of MyD88-independent TIR signalling Cutting Edge: Repurification of Lipopolysaccharide Eliminates Signaling Through Both Human and Murine Toll-Like Receptor 21 Sensitivity of mice to lipopolysaccharide is increased by a high saturated fat and cholesterol diet Inhibitory Effect of Paquinimod on a Murine Model of Neutrophilic Asthma Induced by Ovalbumin with Complete Freund’s Adjuvant Identification of Human S100A9 as a Novel Target for Treatment of Autoimmune Disease via Binding to Quinoline-3-Carboxamides TLR4 Signaling by Heme and the Role of Heme-Binding Blood Proteins Multiomics analysis of naturally efficacious lipid nanoparticle coronas reveals high-density lipoprotein is necessary for their function Understanding the Lipid and Protein Corona Formation on Different Sized Polymeric Nanoparticles Quantitative profiling of the protein coronas that form around nanoparticles Multimodal nanoparticle-containing modified suberoylanilide hydroxamic acid polymer conjugates to mitigate immune dysfunction in severe inflammation Quantification of particle-conjugated or particle-encapsulated peptides on interfering reagent backgrounds Effects of multiple stressors on pancreatic human islets proteome reveal new insights into the pathways involved Effect of PTFGRN Expression on the Proteomic Profile of A431 Cells and Determination of the PTGFRN Interactome Comparative Evaluation of MaxQuant and Proteome Discoverer MS1-Based Protein Quantification Tools Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics Spinal cord injury disrupts plasma extracellular vesicles cargoes leading to neuroinflammation in the brain and neurological dysfunction in aged male mice MetaboAnalyst 5.0: narrowing the gap between raw spectra and functional insights Download references Research reported in this publication was supported the National Institute of General Medical Sciences of the National Institutes of Health under award number R35GM142752 (R.M.P) PhRMA Foundation Predoctoral Fellowship in Drug Delivery under award number 2022 PDDL 877305 (N.T.) University of Maryland School of Medicine’s & Greenebaum Comprehensive Cancer Center’s Flow Cytometry Core–Baltimore This publication was supported by funds through the Maryland Department of Health’s Cigarette Restitution Fund Program and the National Cancer Institute - Cancer Center Support Grant (CCSG) - P30CA134274 University of Maryland School of Medicine’s Center for Translational Research in Imaging – Baltimore University of Maryland School of Pharmacy Mass Spectrometry Center (SOP1841-IQB2014) The content is solely the responsibility of the authors and does not necessarily represent the official view of the National Institutes of Health Marlene and Stewart Greenebaum Comprehensive Cancer Center conceived the project and wrote the manuscript designed and performed the in vitro experiments performed in vivo experiments and sample processing performed the mass spectrometry studies with the supervision of M.A.K performed computational and statistical analyses R.M.P acquired funding and supervised the project All authors edited and approved the final manuscript The authors declare no competing interests Nature Communications thanks the anonymous reviewers for their contribution to the peer review of this work Download citation DOI: https://doi.org/10.1038/s41467-025-56210-4 Renee Corona is an integral part of the team at San Diego SOAR Academy East Mesa.  including teacher and administrative assistant She is currently working as a classroom assistant.  “Her dedication and desire to go the extra mile for our community here at East Mesa makes her an invaluable part of our team,” said Matt Kruger a teacher who works with Corona at SOAR Academy I cannot imagine our site without her.”  That dedication has spurred some of the students at the site to give her the nickname “School Mom.”  “Her commitment to students’ education is tangible and inspiring,” Kruger said Corona to bounce ideas with has made my job easier and more fun.”  Principal Nathan Head said that Corona embodies the resilience and adaptability needed to meet the challenges in the dynamic educational environment at the juvenile detention facility.  “She consistently demonstrates a commitment to and focus on our students by navigating emerging educational platforms and providing essential direct support to our at-promise youth,” he added “She believes in the success of all our students and assists our entire educational staff.”  May is a time when we take a step back to acknowledge and celebrate the many people in education who work hard to ensure students and families are thriving — from principals and teachers to all the staff members behind the scenes — with recognition days and weeks.  Chrys Miller fosters an environment where everyone feels valued Her commitment to the well-being and growth of students and families is why she was chosen SDCOE's 2025 Early Education Teacher of the Year Removing barriers to learning for our students is paramount to helping them succeed but many students don’t realize that poor vision is holding them back Escondido Union High School District is proud to announce and celebrate a historic achievement: three outstanding seniors from Del Lago Academy and Escondido High School who have been selected as 2025 recipients of The Gates Scholarship full-ride scholarship that is granted exceptional high school seniors nationwide each year a virtual event designed to empower students aged 13 and above with practical and creative ways to share their stories and discuss mental health California Pacific Charter Schools’ Junior Leadership Team has been recognized as a 2025 ASCA Student Council School of Excellence by the National Association of Elementary School Principals Metrics details We investigated healthcare avoidance during the first COVID-19 wave in a Dutch region with high infection rates multiphase study used (1) primary care electronic health records to identify patients (2) questionnaires to capture patients with unreported COVID-19 symptoms a natural language model estimated COVID-19 incidence from routine care data 535 (23%) reported COVID-19 symptoms; 180 sought help Care-seeking rates did not differ significantly between those with or without relatives who experienced severe illness or death before their own illness (p = 0.270) Interviews showed the main barriers were feeling not ill enough and concerns about an overstressed healthcare system Only a third of participants with symptoms sought help Serious illness or death of loved ones had no significant impact Findings highlight the need for clear communication and accessible healthcare The underlying reasons for avoiding care during the COVID-19 pandemic remain insufficiently explored the aim of this study was to investigate the impact of COVID-19 on seeking help at the GP during the initial wave of the pandemic We hypothesized that healthcare-seeking behaviour could be influenced by factors such as the serious illness or death of loved ones particularly focused on cases where individuals did not seek help despite having COVID-19 complaints Description of the different phases of our mixed-method study The study population comprised all patients registered at one of the three different general practices in the Dutch municipality Hasselt as of March 2020 meaning the population had slightly changed since 2020 due to factors such as relocation or mortality all inhabitants are registered with one GP Baseline characteristics (March 2020) of all patients were extracted from the electronic health records of the participating practices. Patients with a registered email-address received an online invitation and questionnaire, while others were sent the same materials by post in September 2022 (Supplementary File 1) a reminder was sent to those who received the online questionnaire but not those who received the postal invitation the study was promoted in the local newspaper “De Stentor” The questionnaire was designed to identify patients who experienced COVID-19 symptoms during the first wave but did not seek medical care for their COVID-19 complaints it was an unvalidated questionnaire developed by several experts in the field we identified a subgroup of COVID-19 cases who did not seek medical help participants from this subgroup were randomly selected and stratified across the three different practices they were invited to participate in individual face to face semi-structured interviews at their homes An interview guide was developed based on expert opinion and some available literature at that time to explore the participants’ experiences and needs during the first lockdown period Interviews were conducted by experienced interviewers (AR and ALP) from the regional Community Health Service until no new themes emerged in two consecutive interviews The interviewers were independent of the patients and had no personal interest in study’s outcomes Statistical analysis was performed using SPSS statistics 27.0 software Characteristics of the study population measured on a continuous scale were represented as either mean ± SD or median (interquartile range) Categorical variables were presented as the total number of observations with corresponding percentages Data comparing participants and non-responders were analyzed by using Mann-Whitney U test or Chi Square tests P-values < 0.05 were considered statistically significant The study has been approved by the Medical Ethics Committee of the Isala Hospital The study was conducted in accordance with the Declaration of Helsinki Informed consent was obtained from all participants In total, 6123 patients aged over 16 years were extracted from three different general practices. 143 patients were excluded due to missing data, leaving 5980 patients in the study population. The response rate of the questionnaire was 39% (n = 2361). Baseline characteristics of participants and non-responders are shown in Table 1 The median age of participants was higher than the non-responders (median 57 years the proportion of females was higher in the participant group (58%) compared to the non-responder group (44%) and malignancies exhibited higher response rates those with asthma or COPD had lower response rates Lower response rates were observed in individuals identified by the AI model as having COVID-19 symptoms 4207 have had contact with their GP at least once Information about COVID-19 was discovered in 3400 unique medical records 1508 patients participated in the questionnaire the AI model predicted the presence of COVID-19 Among all participants, 23% (n = 535) reported experiencing any COVID-19 related symptoms (Table 2) 139 participants (26%) actually had a positive PCR-test by testing at the community health service The most frequently reported symptoms were cough (56%) 180 participants (33.6%) reported seeking help for COVID-19 Four patients went directly to the hospital without involving the GP Of the 535 participants with complaints during the first wave 80 participants (15%) reported experiencing symptoms for more than 24 months there was no significant difference in the percentage of relatives or acquaintances who experienced severe COVID-19 symptoms or passed away before their own illness between those who sought care and those who did not (48% vs as mentioned in the free-text section of the questionnaire included ‘due to the wave of participants’ ‘we waited at home to reduce the severity of complaints to a bearable level’ and ‘the government’s advice at the time was: only seek for help if you have severe shortness of breath’ Cross-sectional thematic network of the interrelated themes before the interviews (light grey) and the additional themes resulting from the qualitative analysis (dark grey) of the interviews (n = 12) A key reason for not seeking help was participants’ assumption that they were not seriously ill One participant (#1) said: I do not go to the GP very often and being sick for three weeks and feeling flu-like does not necessarily mean that I will consult a doctor.One participant indicated that he was experiencing ongoing complaints but did not want to burden the GP too much due to the high workload for GPs at that time Participant #5 reflected on the challenging situation for the GPs: But the knowledge that the GP’s were just in such a crisis and were so terribly busy and that you indeed first heard one ambulance after another arriving and later one death bell after another because it was like that.While most participants were satisfied despite not receiving healthcare they were able to manage their symptoms on their own Some indicated they would have sought help if their symptoms had persisted a little longer The mental impact was mainly attributed to the broader COVID-19 crisis in general Several participants described the number of deaths on Hasselt as profoundly impactful and intense Some older participants noted that the infection and crisis had a less impact on them overall as they were already retired and engaged in fewer activities And I have a wife who could always step in if necessary Participants frequently cited social limitations and changes in social interactions as significant factors A participant (#6) commented on the difficult social limitations as well a huge number of people around you have passed away and that had much more impact than me not being able to do my own thing many participants expressed frustration regarding the lack of clarity from the national government and the frequent changes in COVID-19 regulations noting that clearer communication could have alleviated these issues One participant (#4) mentions the lack of appropriate resources to provide good care: But the fact that it just takes a very long time before they realize that you have corona If they had just tested everyone back then And then you could have taken targeted actions but now it was just a bit of muddling through Participants expressed varied preferences for assistance or care but faced barriers like limited complaints and high GP workload They also highlighted healthcare system bottlenecks worsened by COVID-19 such as limiting PCR testing capacity and insufficient aftercare for long-term COVID-19 effects Suggestions for improvement included a 24-h helpline and better guidance on managing complaints and medication use various factors contributed self-direction and self-management among participants Acceptance of their illness and the inability to visit a GP were common themes the majority experienced spontaneous improvement through rest One participant (#5) described the situation as: I am positively surprised by my own ability to deal with it and also to trust the signals my body was giving me and to think I do not need a caregiver at this moment because I am managing Informal support from partners or children including task assistance and emotional support Participants appreciated healthcare workers despite challenges in arranging immediate assistance many understood the crisis constraints and did not blame GPs given the limited understanding of COVID-19 at the time I would just much rather talk directly to a GP but that simply was not there back then either This mixed-methods study suggests that only one-third of patients who experienced COVID-19 related symptoms during the first wave sought medical care no difference was found between healthcare seeking and the presence of serious illness or death of loved ones The primary reason for not seeking help was participants’ belief that they were not sick enough compounded by an overstressed healthcare system Participants generally expressed contentment with most managing the situation by themselves Suggested improvements for future health crises included: sufficient availability of PCR-tests improved access to health advice (possibly through telephone or digital means) and enhanced information on managing complaints and unclear COVID-19 related regulations as key determinants affecting their mental health This study provided meaningful insights in healthcare-seeking behavior in a region heavily impacted by infections during the first wave of the COVID-19 pandemic we did not determine how many people would have actually wanted to seek help Probably some participants who did not seek for help actually had no desire to seek for help because of few complaints the severity of the participants’ complaints remain unknown the questionnaires were sent more than a year after the first wave Applying the AI model on the routine care data resulted in a higher number of participants with a probable COVID-19 episode than the participants themselves recalled meaning the self-reported incidence of COVID-19 could have been underestimated The COVID-19 pandemic has greatly hastened healthcare system transformations Our study findings can enhance care structures for future pandemics emphasizing the need for clear communication and accessible healthcare Data is stored in the online portal ResearchManager Prevalence and determinants of healthcare avoidance during the COVID-19 pandemic: a population-based cross-sectional study E Lockdown policies and the dynamics of the first wave of the Sars-CoV-2 pandemic in Europe Cancer has not gone away: a primary care perspective to support a balanced approach for timely cancer diagnosis during COVID-19 Effects of the COVID-19 pandemic on primary care-recorded mental illness and self-harm episodes in the UK: a population-based cohort study Impact of COVID-19 pandemic on utilisation of healthcare services: a systematic review A Primary health care during the COVID-19 pandemic: A qualitative exploration of the challenges and changes in practice experienced by GPs and GP trainees The role of socio-demographic and health factors during COVID-19 in remote access to GP care in low-income neighbourhoods: a cross-sectional survey of GP patients The general practitioners perspective regarding registration of persistent somatic symptoms in primary care: a survey AR Impact of COVID-19 on Mental Health in Adolescents: A Systematic Review Epidemiology of mental health problems in COVID-19: a review A natural language processing model for COVID-19 detection based on Dutch general practice electronic health records by using bidirectional encoder representations from transformers: development and validation study Data Resource Profile: Registry of electronic health records of general practices in the north of The Netherlands (AHON) Impact of COVID-19 pandemic on mental health in the general population: A systematic review R The association between uncertainty and mental health: a scoping review of the quantitative literature Postponed healthcare in The Netherlands during the COVID-19 pandemic and its impact on self-reported health Revolution in UK general practice due to COVID-19 pandemic: A cross-sectional survey Rural use of health service and telemedicine during COVID-19: The role of access and eHealth literacy Download references The authors thank the participating GPs in Hasselt (Robert van Kleef Nico Tuinstra and Gabriëlle Rook) for sharing their data and for their assistance in contacting the patients Special thanks are extended to Fokko Douma for his IT-support Acknowledgement is also given to Marieke Jansen for conducting the interviews we extend our appreciation to Lilian Peters Maarten Brilman and Karina Sulim for their contributions in running the AI model wrote the manuscript in consultation with E.A.G. was responsible for conducting and processing the interviews assisted us with obtaining the questionnaires and processing the data The Hasselt Corona Impact Study has been approved by the Medical Ethics Committee of the Isala Hospital Zwolle (registration number 20211017) Download citation DOI: https://doi.org/10.1038/s41533-025-00426-w Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily. No one has been better than star pitcher Seth Hernandez. He entered Monday having struck out 15 with no walks and no runs in eight innings. Against Norco, he hit a three-run home run and struck out 12 and gave up two hits and a walk over five innings in a 9-0 victory over Norco in the Panthers’ Big VIII League opener. Anthony Murphy also homered for the Panthers. Ethin Bingaman and Brady Ebel each had two hits. Corona Centennial 7, Corona Santiago 6: The Huskies, trailing 6-0, scored five runs in the fifth inning, one in the sixth and got a walk-off bases loaded walk in the seventh to win the Big VIII League game. Mason Lear had a three-run double. High School Sports Louis Lappe, star of 2023 Little League World Series champion El Segundo, will play high school baseball at Harvard-Westlake. King 5, Eastvale Roosevelt 4: A three-run sixth inning helped King win. Castaic 6, West Ranch 4: Freshman Orion Gonzalez contributed a home run, double, single and four RBIs to help Castaic win its Foothill League opener. Gio Foster also homered. Ty Diaz led West Ranch with three RBIs. Sylmar 13, San Fernando 1: The Spartans opened Valley Mission League play with an impressive win. Victor Espinoza and Martin Magana each had two RBIs. Alex Martinez struck out seven in 4 1/3 innings and had three RBIs. Sun Valley Poly 10, Kennedy 0: Mark Cesena allowed one hit and struck out eight in 6 2/3 innings for Poly. Gael Garcia had two hits and three RBIs. Fullerton 3, Villa Park 2: Playing at Angel Stadium, Miguel Velasquez of Fullerton hit a memorable first-inning home run for Fullerton. Declan Fitzgerald struck out eight in six innings for Fullerton. Calabasas 11, Taft 1: Connor Pink hit two home runs to lead Calabasas. Luc Olson threw five scoreless innings. Banning 4, Carson 0: Anthony Camarena threw six shutout innings with seven strikeouts and also had two hits for Banning in the Marine League win. San Pedro 13, Narbonne 5: The Pirates won their Marine League opener. Anthony Solis hit two doubles and had five RBIs and Braydon Griffin finished with three hits. Granada Hills 10, Newbury Park 2: Tristan Jennings had three hits and Anthony Castro and Landon Tuch each had three RBIs for Granada Hills. Royal 14, Oak Park 2: Brady Hewitt and Isaiah Tillman each had two hits and three RBIs for Royal. Summit 10, Beckman 0: Isaac Castanon had a home run and three RBIs for Summit. Orange. Lutheran 9, Las Vegas Centennial 0: Brady Murrietta and Josiah Hartshorn hit home runs for the Lancers in Las Vegas. Arcadia 6, Damien 4: The Apaches improved to 10-0 with a win at Angel Stadium. Tyler Swilling got the save. Los Altos 6, Simi Valley 1: Freshman Kaylahni Duenas struck out nine for 6-1 Los Altos. Chaminade 11, Oak Park 4: Norah Pettersen hit a home run and had three RBIs for Chaminade. Eric Sondheimer is the prep sports columnist for the Los Angeles Times. He has been honored seven times by the California Prep Sportswriters Assn. for best prep sports column. 85%;">Corona 100 This Is Living Since 1925 View source version on businesswire.com: https://www.businesswire.com/news/home/20250425804516/en/ Already have an account? Login