It is with heavy hearts that we announce the passing of Carol Kay Franke Guzman and cherished member of the Yorktown Community Whether it was helping special needs kids at the school in Yorktown taking care of patients as a nurse at Yorktown Medical Clinic or sitting with elderly patients in their home she was a constant source of support and warmth Her home was always open to anyone who needed a place of comfort and her wisdom and love were a guiding light to all who were fortunate enough to know her Carol had a profound impact on the lives of many Her nurturing spirit and tireless commitment to making the world a better place was unmatched Through her selflessness and unwavering love for others she showed us all the true meaning of community and care will continue to inspire those she leaves behind Carol is survived by her children Larissa (Brent) Niemeier Ashleigh (Travis) Peyton and Meghan (Adam Cortez) Davis She is also survived by her great-grandchildren; Lucas great grandson Caden Reynolds and sister Junell Suggs Honorary pallbearers will be granddaughters Visitation will be held from 5-7 pm on Thursday April 24 Funeral Service will be 10 am Friday April 25 the family requests donations be made to St Andrew’s Lutheran Church in Weesatche in her memory You are invited to sign the online guestbook at www.masseyfh Read Cuero Record Read Yorktown News-View © 2025 DeWitt County Today passed away peacefully at Touchmark Community daughter of the late Harold and Martha (Ponzer) Reinert Jean was baptized in the comfort of her home with Mrs Irvin Paul and Reinhardt Ponzer as her sponsors she was confirmed at Grace Lutheran Church in Sugar Bush. Jean’s confirmation verse was Romans 12:21: “Do not be overcome by evil but overcome evil with good.” Jean was preceded in death by her husband of 53 years Anton “Pete” De Witt; her brother Anton and Minnie (De Groot) De Witt; four sisters-in-laws: Carol De Witt and Theresa Halverson; two brothers-in-law: Bob De Witt where she began her career at Carlson’s Restaurant located at the intersection of Locust St and Wisconsin Ave she married the late Anton “Pete” De Witt and was married for 53 years Jean was a dedicated member of Christ the King Church for over 30 years also taking on various cleaning jobs in the evenings Her passion for service flourished when she found fulfillment as a waitress where she formed lasting friendships with patrons and coworkers alike planting flowers and taking long walks; however her greatest joy came from being surrounded by her family and spunk will be profoundly missed by all who had the privilege of knowing her She lived a life of unwavering kindness and generosity and was known for her warm hospitality; no one ever left her home without something delicious to eat often accompanied by a bag of your favorite cookies she dedicated herself to her children and grandchildren her love shone through as she would ask about the grandchildren inquiring playfully if they had been naughty and breaking into a warm smile that reflected her enduring spirit and joy in their lives Jean is survived by her children: Mark (Kay) Beresford Kevin (Wendy Van Der Geest) De Witt Darin (Michele) De Witt and stepson Paul De Witt She is also survived by her 11 cherished grandchildren: Sara (Derek) Van Der Horst Connor De Witt and Ember De Witt; step-grandchild Leah (Luke) Peerenboom; and 17 great-grandchildren: Owen (Maggie) Jean is further survived by two brothers-in-law The family wishes to express their heartfelt gratitude to the staff at Touchmark Retirement Community and St Croix Hospice for their compassionate care and Paster Nate Gauerke A message to the grandchildren and great-grandchildren “There isn’t a future in the past but you can do something about tomorrow.” Additionally she leaves you with words of Proverbs 3:5: “Trust in the Lord with all your heart and lean not on your own understanding.” A private memorial will be held at a later date Please select what you would like included for printing: Copy the text below and then paste that into your favorite email application passed away at the age of 92 on February 19 daughter of the late Albert and Alta Johnson Ann was preceded in death by her husband of 49 years grandmother of nine and great-grandmother of nine and important dates in her family's lives She was a member of Atonement Lutheran Church in Muskego Vacation Bible school and enjoyed the fellowship of her women's Bible studies She was an avid reader and book club enthusiast Ann is survived by her three daughters- Paula Wike (Paul) of St She will be greatly missed by her nine grandchildren and great-grandchildren A memorial service will be held at 11:00 am on Saturday memorial contributions may be made to Atonement Lutheran Church my good and faithful servant" Matthew 25:23 Add to Calendar Complete the form below to get directions for the Funeral Service for Ann M This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply Service map data © OpenStreetMap contributors This website is using a security service to protect itself from online attacks The action you just performed triggered the security solution There are several actions that could trigger this block including submitting a certain word or phrase You can email the site owner to let them know you were blocked Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page Texas to James Hetrick and Bettye Eastwood Kathy is survived by her children Mathew (Sally) Weischwill She is also survived by her brother Kelley Hetrick Kathy is preceded in death by her Husband Ron Weischwill Memorials may be donated to Alzheimer’s Association 1100 Northwest Loop 410 Texas 78213 or Donor’s Choice You are invited to sign the online guestbook at www.masseyfh com Services by Massey Funeral Home 361564-2900 a beautiful little girl was born to Louis and Roseline Rangnow and they named her Eileen Rose Rangnow She was able to spend 2 years with her dad before he passed away on February 17 Her mother remarried to Raymond Hardt on November 27 Growing up Eileen struggled with asthma which often kept her out of school she prayed for God to send her a man to take her away and take care of her Her prayer was answered when she found that man at a dance in Nordheim Finley and Eileen were married on September 2 their son Ricky Don Barth was born and in 1968 Eileen started to babysit children which led to opening a daycare Later in life they retired and moved to Cuero Eileen shifted from caring for kids to caring for elderly relatives and friends She was actively involved in the church in Woodsboro and then in Lindenau She started Sunday brunches and became known as “the church lady” that would help set up for weddings Eileen and her daughter initiated a once a month Senior Citizens Luncheon in Lindenau to provide a place for seniors to socialize and “break bread together” Matthew Barth and Sean (Vanessa) Burge; great granddaughter nephews and great nieces and great nephews She is preceded in death by her grandparents 2025 from 5:00 – 7:00 PM at Freund Funeral Home 2:00 PM at Freund Funeral Home with Pastor Jan R Interment will follow at Lindenau Community Cemetery Driscoll Children’s Hospital or C.A.M.A.L House You may sign the guestbook or send condolences at www TEA releases 2023 A-F accountability ratings To access content, please login or purchase a subscription attended the Cuero Chamber of Commerce Ag Luncheon on April 23 Ag students who opened the luncheon included To access content, please login or purchase a subscription Lady Gobblers celebrate after beating Pleasanton in two games on Thursday and Friday making them Bi-District Champions To access content, please login or purchase a subscription All DeWitt County fire departments trained on car fires at the Friar Ag Center on April 21 The Volunteer Fire Departments in all locations invite citizens to join in keeping communities safe To access content, please login or purchase a subscription Coach Karl Saenz visits with the team after winning the game vs Gobbler catcher Kyle Bias waits on a pitch during the district match-up between Cuero and LaVernia on Tuesday April 22 To access content, please login or purchase a subscription Art students shine thanks to Education Foundation grant To access content, please login or purchase a subscription To access content, please login or purchase a subscription Phelps charges related to a recent county vehicle wreck To access content, please login or purchase a subscription FES students see career opportunities on wheels Students at French Elementary explored several career options at Career Day last Thursday The John Deere utility truck with attachments is used for plowing To access content, please login or purchase a subscription Anthony Rath (L) & Green Cow Creamery Owner Rudy Rocha (R) are giving Em a new home at the Pecan House Café (Ele Chew) To access content, please login or purchase a subscription Director of special program Ruby Rodriguez with School Climate Specialists Courtney Kalinowski and Lacy Timpone raise awareness of the availability of Mental health resources To access content, please login or purchase a subscription Children at the 38th Annual Nordheim Easter Egg Hunt met Penny Cottontail To access content, please login or purchase a subscription Metrics details are widely used in the textile and automotive industry due to their high durability and tensile strength Chemical recycling of polyamides is possible but typically yields mixtures of monomers and oligomers which hinders downstream purification Pseudomonas putida KT2440 was engineered to metabolize C6-polyamide monomers such as 6-aminohexanoic acid ε-caprolactam and 1,6-hexamethylenediamine Heterologous expression of nylonases also enabled P putida to metabolize linear and cyclic nylon oligomers derived from chemical polyamide hydrolysis RNA sequencing and reverse engineering revealed the metabolic pathways for these non-natural substrates the phaCAB operon from Cupriavidus necator was heterologously expressed to enable production of polyhydroxybutyrate (PHB) from PA6 hydrolysates This study presents a microbial host for the biological conversion of polyamide monomers and mixed polyamids hydrolysates to a value-added product putida is not capable of metabolizing PA-related monomers or oligomers which has prevented their upcycling until now we used deep metabolic engineering guided by laboratory evolution to enable metabolism of prevalent PA monomers RNA sequencing (RNA-seq) was performed to identify key enzymes and transporters of the engineered metabolic pathways and heterologous expression of the nylonase-encoding genes nylABC from P ureafaciens extended the substrate range to linear and cyclic Ahx oligomers The engineered strain fully metabolized hydrolysates of PA6 and was further engineered to convert them into polyhydroxybutyrate (PHB) and other value-added products our work provides a powerful host enabling the microbial upcycling of PA monomers and complex PA6 hydrolysates thereby overcoming the drawbacks of traditional recycling processes and leading the path towards sustainable end-of-life solutions for synthetic PA Conceptual overview of PA bio-upcycling through hydrolysis and microbial catalysis illustrating the chemical structures of monomers and exemplary oligomers derived from different PA substrates putida KT2440-AA and HMDA-1 mutant on diamines ALE of PP_2884∆3 on Ahx as sole carbon source Shown are the growth profiles of five subsequent cultures Time points of re-inoculation are indicated Growth of mutants resulting from ALE on Ahx as sole carbon source Target mutations (red asterisks) for reverse engineering yielded by genome sequencing of strain Ahx-194 Comparative analysis of the growth of the ALE and reverse-engineered strains on HMDA Strains were cultivated in Growth Profiler in 96-well microtitre plates with MSM containing 15 mM of HMDA Ahx or ε-caprolactam as sole carbon source Source data the transcriptional regulator probably acts as a repressor and the deletion of F61 F62 and S63 within the DNA-binding domain might abolish the ability to bind its regulatory targets When both modifications were introduced into wild type P none of the C6-PA monomers were metabolized metabolism of the C6-PA monomers by the ΔPP_2884 and PP_2884Δ3 mutants occurred via AA requiring the previous modifications of the P PP_0409-10 might also activate expression of the downstream-located PP_0411–4 encoding a putative polyamine ABC transporter for C6-PA monomer import that is analysed below Statistical analysis was performed using the Exact Test for two-group comparisons Effect of the clean deletion (Δ) or base-editing mediated functional disruption (*) of genes putatively involved in γ-glutamylation and/or transamination on growth with HMDA Formation of alanine from pyruvate or glutamic acid from α-KG by purified transaminase candidates in the presence of HMDA and Ahx Effect of oplBA (PP_3514-5) deletion on growth with ε-caprolactam compared to the parent strain Green arrows behind locus tags indicate upregulated genes triangles indicate (sets of) genes investigated by knockout coloured according to the phenotype (green Green checkmarks indicate confirmed reactions with isolated enzyme in vitro Activity of the greyed enzymes could not be proven in vitro Source data putida’s metabolism towards synthetic compounds was revealed which enables growth on nylon monomers through exclusively native metabolic enzymes activated by two key mutations affecting transcriptional regulation of a wide variety of genes a–c, Strains were grown in MSM with 15 mM Ahx2 (a), Ahx3 (b) or a soluble PA6 fraction containing ε-caprolactam, Ahx and linear Ahx oligomers (c) as sole carbon and nitrogen source. The composition of the soluble PA6 fraction is shown in Extended Data Fig. 9 HPLC chromatogram showing the separation of ε-caprolactam (1) and cyclic Ahx oligomers (2–6) Peak numbers correspond to the size (n) of the Ahx oligomers Compounds were detected with a diode array detector (DAD) at λ = 210 nm HPLC chromatogram of Ahx (1) and linear Ahx oligomers (2–7) Terminal R-NH2 groups were pre-column derivatized using o-phthaldialdehyde (OPA) The derivatives were detected with a fluorescence detector (FLD) with excitation λ = 340 nm and emission λ = 450 nm HPLC analysis of culture supernatants of P red) cultivated with the soluble PA6 fraction as sole carbon and nitrogen source The concentrations of ε-caprolactam and Ahx equivalents (Ahxeq) of Ahx and its linear oligomers are shown for indicated cultivation times Source data nylC or nylABC were cultivated with cyclic Ahx2–6 as sole carbon and nitrogen source putida NYLON-ABC that was found to only metabolize cyclic Ahx2 Crude extract assays of different nylonase-expressing strains with cyclic Ahx2–6 The peak area of cyclic Ahx oligomers (c) and concentration of linear Ahx oligomers (d) is shown after a reaction time of 6 h Source data HPLC chromatogram showing the release of soluble Ahx and Ahx2–7 upon acidic hydrolysis after the indicated hydrolysis time and relative composition in final MSM medium that was used to produce PHB Peak numbers correspond to the size (n) of Ahxn dimer (d) and monomer (e) in culture supernatants and growth (f) of P concentrations in both replicates are shown along with the mean indicated by the line Percentage of PHB in the cell dry weight (CDW) of P putida NYLON-PHB grown on pure Ahx and PA6 hydrolysate The strain was cultivated in 50 ml MSM supplemented with 30 mM of Ahx or of PA6 hydrolysate adjusted to be equimolar to 30 mM of monomeric Ahx Source data A drop collapse assay with 50 µl culture supernatants on polystyrene (middle) and a TLC of ethyl acetate extracts of culture supernatants (right) indicated the presence of serrawettin W1 biosurfactant; spots were visualized with 60% H2SO4 putida NYLON EV carrying an empty vector cultured and treated identically as the expression strains served as control The strains were cultivated in MSM medium supplemented with PA6 hydrolysate adjusted to be equimolar to 30 mM of monomeric Ahx Source data we used deep metabolic engineering guided by laboratory evolution to establish the synthetic metabolism of PA-related feedstocks in the microbial host P This yielded the highly engineered strain P putida NYLON-ABC that is able to funnel various PA monomers as well as cyclic and linear oligomers of PA6 into its central metabolism RNA-seq revealed the metabolic pathways of HMDA Ahx and ε-caprolactam including key regulators and transporters most modifications could be obtained through laboratory evolution highlighting the versatile metabolism of pseudomonads and hinting at how natural evolution could occur on nylon pollutants violacein and serrawettin from C6-PA monomers and PA6 hydrolysates was successfully demonstrated by introduction of their respective biosynthetic pathways we establish PA hydrolysates as potentially broadly applicable biotechnological feedstock providing an end-of-life option for PA wastes such as fishing nets and clothing There is therefore an urgent need for feedstock-flexible recycling technologies that can better deal with mixtures of chemicals and materials This store of knowledge can be leveraged by our work which unlocks production of these chemicals from millions of tons of plastic waste that are currently landfilled and incinerated by enabling a transition from sugars to (poly)amides as feedstock This could enable upcycling of mixed-plastic waste streams without previous sorting by establishing them as feedstock for biological catalysts revolutionizing the end-of-life solutions not only for pure plastics but also polymer blends and mixtures that are currently not amenable to traditional recycling and culture supernatants were extracted with ethyl acetate after another 44 h of cultivation For protein production in Escherichia coli BL21(DE3) strains main cultures were inoculated by diluting broth of an overnight preculture 1:50 with LB medium containing 50 µg ml−1 kanamycin Expression of the tagged enzymes was induced by adding IPTG at a final concentration of 0.5 mM Incubation was continued at 18 °C overnight cultures were centrifuged at 4,000 × g and 4 °C for 10 min Cells were regenerated in 900 µl LB medium 100 µl of this cell suspension was used to inoculate 100 ml shake flasks each filled with 10 ml LB containing kanamycin These cultures were incubated to allow for base editing and used to re-inoculate subsequent cultures (with kanamycin) to extend the time for base editing if required the different cultures were used to inoculate 10 ml LB containing 10% (w/v) sucrose to induce SacB-mediated plasmid counterselection Single colonies were isolated on LB plates and replica picked onto LB and LB containing kanamycin to screen for the loss of the plasmid as indicated by kanamycin sensitivity Subsequent colony PCRs of single colonies lysed in alkaline PEG200 using OneTaq poylmerase were performed to amplify the target regions PCR products were purified and analysed by Sanger sequencing to identify the intended mutations For the integration of codon-optimized nylonase genes from P. ureafaciens into P. putida KT2440, the intergenic region of PP_0340-1 was chosen as landing pad, as the attTn7-site was already occupied due to previous modifications enabling growth on adipate. The sequences of the codon-optimized genes are shown in Supplementary Table 7 ALE was performed in 96-well microtiter plates with transparent bottom (Enzyscreen CR1496dg) in Growth Profiler by iterative inoculation of MSM with 15 mM substrate as indicated Four different ALE experiments were performed in parallel (A1–A4) the cell culture of a well was diluted 20-fold (10 µl in 190 µl) into fresh medium in a new well The sequential cultivations were performed until no further increase in growth was observed single clones were isolated on LB agar plates and growth of individual clones was verified on PA monomers The best-growing strains were subjected to whole-genome sequencing Genomic DNA of selected strains was purified using a Monarch Genomic DNA Purification kit (NEB) from an overnight LB culture 1 μg of DNA was used for library preparation using the NEBNext Ultra II DNA Library Prep kit for Illumina (NEB) The library was evaluated by qPCR using the KAPA library quantification kit (Peqlab) normalization for pooling was done and paired-end sequencing with a read length of 2 × 150 bases was performed on a MiSeq system (Illumina) The sequencing output (base calls) were received as demultiplexed fastq files coverage extraction) were processed using the CLC Genomic Workbench software (Qiagen Aarhus A/S) Reads were mapped against modified versions of the P putida KT2440 genome that included the genomic integrations The relevance of identified mutations was assessed manually Sequencing data are stored in the NCBI Sequence Read Archive under BioProject PRJNA1023861 CLC Genomics Workbench v.20 was likewise applied to visualize differential expression as volcano plots To analyse linear substrates harbouring one or two primary amines pre-column derivatization using o-phthaldialdehyde (OPA) reagent (Sigma-Aldrich For separation of the derivatized molecules the Kinetex 2.6 μm EVO C18 100 Å column (100 × 2.1 mm) (Phenomenex) was used 10 mM sodium-borate buffer (A) (pH 8.2) and methanol (B) were used (70% A–30% B) applying increasing gradients of methanol (70% B after 10 min The flow was adjusted to 0.4 ml min−1 at 40 °C Derivatized molecules were detected using an FLD with an excitation of λ = 340 nm and an emission of λ = 450 nm and assigned with the help of analytical standards Ahx equivalents in oligomers were calculated by multiplying the detected Ahx-oligomer concentration with the size (n) of the corresponding oligomer Detection of cyclic Ahx oligomers was performed using the DAD with an absorption of λ = 210 nm (reference λ = 300 nm) Cyclic oligomers of Ahx were separated using a Zorbax Eclipse XDB-C8 column (4.6 × 150 mm) with a H2OMilliQ:MeOH ratio of 60:40 and a constant flow of 0.5 ml min−1 at 40 °C As no analytical standards were available for cyclic Ahx oligomers the corresponding peak area was analysed allowing semi-quantitative analysis The soluble fraction of PA6 was obtained from PA6 pellets (B4Plastics) by stirring 100 g l−1 of PA6 material in H2OMilliQ for 72 h the sample was filtered using a bottle top filtration unit (0.22 µM PES membrane) The filtrate was subjected to a rotary evaporator (Rotavapor R-210 Büchi) for 4 h at 40 °C and 60 mbar to obtain a white putida strains were collected during the exponential phase (OD600 = 2.0) from an MSM cultivation supplemented with 20 mM glucose Four millilitres of culture was centrifuged at 21,000 × g at 4 °C for 2 min The cell pellet was resuspended in 2 ml of 100 mM phosphate buffer (pH 7.5) ultrasonication was performed using a UP200S ultrasonicator (Hielscher) with an amplitude of 55 To remove cell debris from the crude extracts samples were centrifuged at 21,000 × g at 4 °C for 10 min 500 µl of the obtained supernatant was mixed with 500 µl of cyclic Ahx2–6 solution (7 mg ml−1) that was filtered through a 0.22 µM PES filter membrane cell pellets were resuspended in 1 ml binding buffer (1.76 g l−1 Na2HPO4 pH 7.4) and supplemented with 0.2 mg ml−1 lysozyme samples were additionally lysed mechanically using the Precellys homogenizer with silica beads Protein purification was performed with His SpinTrap columns (Cytiva) according to manufacturer instructions Buffer of the purified protein was exchanged with double-distilled H2O via Zeba Spin Desalting Columns (Thermo Fisher) The transaminase assays were carried out in 100 mM sodium phosphate buffer (pH 7.5) with 500 µM acceptor substrate (pyruvate or α-ketoglutarate) 5 mM donor substrate (HMDA or Ahx) and 5 µM purified enzyme H2O was used instead of amine donor solution The reaction mixtures were incubated at 30 °C for 30 min and stopped by boiling at 100 °C for 10 min The reaction was followed either by photometric measurements using the Alanine Assay kit (Cell Biolabs) according to manufacturer protocol or by HPLC (glutamate) One gram of PA6 pellets was incubated in 10 ml of 50% (v/v) H2SO4 for 24 h at 100 °C using an oil bath the soluble hydrolysate was filtered through a 0.22 µM PES filter membrane and subsequently diluted 10-fold with H2OMilliQ Ca(OH)2 was added under agitation until a pH of 7 was reached The precipitating CaSO4 was removed by filtration through a 0.22 µM filter paper components of MSM were added to this mixture and filtered through a 0.22 µM PES membrane filter to obtain the sterile PA6 hydrolysate for growth and PHB production experiments Molecules and protein complexes were visualized using ChemDraw (PerkinElmer) Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article Plastics - The Fast Facts 2023 (Plastics Europe, 2023); https://plasticseurope.org/knowledge-hub/plastics-the-fast-facts-2023/ Preferred Fiber and Materials Market Report (Textile Exchange Chemical recycling processes of nylon 6 to caprolactam: review and techno-economic assessment Chemical recycling of end-of-life polyamide 6 via ring closing depolymerization Mechanical recycling of an end-of-life automotive composite component Critical advances and future opportunities in upcycling commodity polymers Synthesis of high molecular weight nylon 6 by anionic polymerization of ε-caprolactam Utilization of a cyclic dimer and linear oligomers of ϵ-aminocaproic acid by Achromobacter guttatus KI 72 Chemical and biological catalysis for plastics recycling and upcycling Mixed plastics waste valorization through tandem chemical oxidation and biological funneling The metabolic potential of plastics as biotechnological carbon sources – review and targets for the future Characterization of the caprolactam degradation pathway in Pseudomonas jessenii using mass spectrometry-based proteomics Metabolic pathway of 6-aminohexanoate in the nylon oligomer-degrading bacterium Arthrobacter sp KI72: identification of the enzymes responsible for the conversion of 6-aminohexanoate to adipate Catabolism of biogenic amines in Pseudomonas species A novel putrescine utilization pathway involves gamma-glutamylated intermediates of Escherichia coli K-12 Biotransformation of nylon-6,6 hydrolysate to bacterial cellulose 6-Aminohexanoate oligomer hydrolases from the alkalophilic bacteria Agromyces sp and characterization of nylon-6 enzymatic depolymerization Purification and characterization of 6-aminohexanoic-acid-oligomer hydrolase of Flavobacterium sp Increasing the diversity of of nylonases for poly(ester amide) degradation A new nylon oligomer degradation gene (nylC) on plasmid pOAD2 from a Flavobacterium sp on plasmid pOAD2 from a Flavobacterium strain encodes endo-type 6-aminohexanoate oligomer hydrolase: purification and characterization of the nylC gene product Biological treatment of the components of solid oligomeric waste from a nylon-6 production plant Engineering adipic acid metabolism in Pseudomonas putida Unraveling 1,4-butanediol metabolism in Pseudomonas putida KT2440 Laboratory evolution reveals the metabolic and regulatory basis of ethylene glycol metabolism by Pseudomonas putida KT2440 Engineering Pseudomonas putida KT2440 for efficient ethylene glycol utilization Characterization and engineering of branched short-chain dicarboxylate metabolism in Pseudomonas reveals resistance to fungal 2-hydroxyparaconate Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates Towards bio-upcycling of polyethylene terephthalate Integration of genetic and process engineering for optimized rhamnolipid production using Pseudomonas putida Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440 Specific gene expression in Pseudomonas putida U shows new alternatives for cadaverine and putrescine catabolism Molecular characterization of PauR and its role in control of putrescine and cadaverine catabolism through the γ-glutamylation pathway in Pseudomonas aeruginosa PAO1 Functional characterization of the agtABCD and agtSR operons for 4-aminobutyrate and 5-aminovalerate uptake and regulation in Pseudomonas aeruginosa PAO1 Functional role of lanthanides in enzymatic activity and transcriptional regulation of pyrroloquinoline quinone-dependent alcohol dehydrogenases in Pseudomonas putida KT2440 Redundancy in putrescine catabolism in solvent tolerant Pseudomonas putida S12 Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted γ-Glutamylputrescine synthetase in the putrescine utilization pathway of Escherichia coli K-12 Nitrogen metabolism in Pseudomonas putida: functional analysis using random barcode transposon sequencing Omics-driven identification and elimination of valerolactam catabolism in Pseudomonas putida KT2440 for increased product titer Three-dimensional structure of nylon hydrolase and mechanism of nylon-6 hydrolysis Transport and kinase activities of CbrA of Pseudomonas putida KT2440 Hierarchical management of carbon sources is regulated similarly by the CbrA/B systems in Pseudomonas aeruginosa and Pseudomonas putida Lack of CbrB in Pseudomonas putida affects not only amino acids metabolism but also different stress responses and biofilm development Functional analysis and regulation of the divergent spuABCDEFGH-spuI operons for polyamine uptake and utilization in Pseudomonas aeruginosa PAO1 Exploring engineered vesiculation by Pseudomonas putida KT2440 for natural product biosynthesis Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX A straightforward assay for screening and quantification of biosurfactants in microbial culture supernatants Review of polymer technologies for improving the recycling and upcycling efficiency of plastic waste Chemistry and materials science for a sustainable circular polymeric economy Validated high-throughput screening system for directed evolution of nylon-depolymerizing enzymes Pseudomonas putida as a functional chassis for industrial biocatalysis: from native biochemistry to trans-metabolism Bio-upcycling of viscose/polyamide textile blends waste to biopolymers and fibers Bio-upcycling of even and uneven medium-chain-length diols and dicarboxylates to polyhydroxyalkanoates using engineered Pseudomonas putida Engineering of solvent-tolerant Pseudomonas putida S12 for bioproduction of phenol from glucose Comparison of methods for measurement of bacterial growth rates in mixed batch cultures Enzymatic assembly of DNA molecules up to several hundred kilobases CRISPy-web: an online resource to design sgRNAs for CRISPR applications Studies on transformation of Escherichia coli with plasmids Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440 Metabolic engineering of Pseudomonas taiwanensis VLB120 with minimal genomic modifications for high-yield phenol production A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes Small-sample estimation of negative binomial dispersion Controlling the false discovery rate: a practical and powerful approach to multiple testing Microbial production of the drugs violacein and deoxyviolacein: analytical development and strain comparison Biosynthesis of violacein: a genuine intermediate Heterologous production of the lipopeptide biosurfactant serrawettin W1 in Escherichia coli SAPPHIRE: a neural network based classifier for σ70 promoter prediction in Pseudomonas Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes ColabFold: making protein folding accessible to all Database resources of the National Center For Biotechnology Information Enhanced annotations and features for comparing thousands of Pseudomonas genomes in the Pseudomonas genome database Download references received funding for this study from the European Union’s Horizon 2020 research and innovation programme under grant agreement numbers 887711 (Glaukos) as well as from the European Research Council under grant number 101044949 (PROSPER) received further funding from the Deutsche Forschungsgemeinschaft (DFG German Research Foundation) under project ID 458090666 for CRC1535/1 Nikel (Technical University of Denmark) for providing pSEVA6311::phaCAB; L Sundermeyer for the violacein expression plasmid Germany) for reference materials of violacein Japan) for providing cyclic Ahx oligomers as substrates; and B4Plastics (Dilsen-Stokkem Open access funding provided by Forschungszentrum Jülich GmbH Institute of Bio- and Geosciences IBG-1: Biotechnology performed data curation and formal analysis acquired resources and supervised the project conducted formal analysis and data curation a major manufacturer of industrial enzymes No patents based on the results of this study were applied for All other authors declare no competing interests Nature Microbiology thanks Wei Niu and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations The DNA-binding domain is colored in blue and amino acids deleted in PP_2884Δ3 are labelled red and displayed as sticks (F61 a-c, Single clones were obtained from adaptive laboratory evolution of PP_2884Δ3 on Ahx and screened in MSM supplemented with 15 mM of HMDA (a) and ε-caprolacton (c). Growth on these substrates is shown is shown in comparison to 15 mM Ahx (b) as also shown in Fig. 1d ALE mutant Ahx-194 that was selected for whole-genome sequencing is highlighted in green Source data All strains were cultivated in MSM supplemented with 15 mM of HMDA (a) Deletion of PP_0409-10 (red) resulted in decreased growth with all C6-PA monomers whereas replacement by the constitutive promoter P14b upstream of PP_0411-4 (ΔPP_0409-10::P14b) (orange) increased growth The mean values and standard deviations of three replicates are shown (n = 3) Source data Deletion of PP_0410-4 resulted in decreased growth with all C6-PA monomers Source data The strains were cultivated in MSM supplemented with 15 mM of HMDA (a) and Ahx (b) Deletion of oplBA (orange) did not significantly alter growth with HMDA and Ahx Source data Single clones were obtained from adaptive laboratory evolution of PP_2884Δ3 P14f -nylB on Ahx2 and screened in MSM supplemented with 15 mM of the oligomers Ahx2 (a) and Ahx3 (b) ALE mutant Ahx2-322 (green) and ALE mutant Ahx2-323 (blue) were selected for whole-genome sequencing Source data Genetic context of the identified mutation in ALE mutant Ahx2-322 The mutation (C→T) (red flash) was located in close proximity to the predicted promoter regions of PP_2176 (green) and PP_2177-80 (blue) its repressor PP_2181 is encoded that represses expression of the operon in the presence of HMDA and other amines The mutation might enable constitutive expression of PP_2177-80 or prevent binding of PP_2181 thereby activating expression of PP_2177-80 in the absence of inducers Promoter regions were predicted using SAPPHIRE Combinations of the identified mutations were implemented in P putida NYL P14f –nylB and screened individually or in combination yielding the final reverse engineered strain P Strains were cultivated in MSM supplemented with 15 mM of Ahx2 (b) and Ahx3 (c) or the PA6 soluble fraction (d) Source data HPLC chromatograms showing the separation of Ahx and linear Ahx2-7 (a) or ε-caprolactam (cyclic Ahx1) and cyclic Ahx2-6 (b) that were detected using FLD and DAD The concentration of the indicated compounds is shown no standards were available preventing their quantification putida NYLON-B on acidic hydrolysate of PA66 Consumption of amine substrates in Nylon66 hydrolysate by P Mean values and standard deviations of three replicates are shown (n = 3) Concentration of amine monomers and oligomers was determined via HPLC after amino group derivatisation with OPA Source data putida strains on different carbon sources putida NYL compared to unevolved KT2440-AA under adipic acid-metabolizing conditions Significant hits (FDR P < 0.01 and |log2 fold change| > 1) are shown For statistical analysis of differential gene expression an Exact Test for two-group comparisons with default settings of CLC Genomics Workbench was performed The FDR P value corrects for multiple comparisons putida NYL under Ahx-metabolizing conditions compared to adipic acid-metabolizing conditions Overview of strains used and generated in this study DNA sequences of codon-optimized nylonase-encoding genes for P Numerical values of OD equivalents determined by growth monitoring using Growth Profiler (Enzyscreen) for Fig Numerical values of amino acid concentration as determined by Kit and HPLC-FLD for Fig Numerical values of concentrations of ε-caprolactam and Ahx equivalents (Ahxeq) of Ahx and its linear oligomers as determined by HPLC-FLD/DAD for Fig Numerical values of concentrations of and Ahx equivalents (Ahxeq) of Ahx and its linear oligomers as determined by HPLC-FLD/DAD for Fig 5f and numerical values of CDW and PHB-monomer for Fig Numerical values of concentrations of violacein for Fig and uncropped pictures of TLC plates after development and staining as shown in Fig Numerical values of OD equivalents determined by growth monitoring using Growth Profiler (Enzyscreen) for Extended Data Fig Numerical values of concentrations of HMDA and Nylon66 linear oligomers as determined by HPLC-FLD/DAD for Extended Data Fig Download citation DOI: https://doi.org/10.1038/s41564-025-01929-5 Anyone you share the following link with will be able to read this content: a shareable link is not currently available for this article Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology Enter your phone number above to have directions sent via text Tennessee and daughter of Aaron and Felicia DeWitt and Chelsea Cook Emily was known for her unwavering smile that could brighten any room She was always willing to lend a helping hand to anyone around her Her generous heart made her a friend to many and her compassion knew no bounds Her love for her family was profound and she cherished the special bond she shared with her cousins they created memories filled with laughter and joy and Emily’s genuine affection for them was evident in every moment they spent together Pink was her favorite color and it was a fitting tribute to her vibrant personality Emily had a passion for makeup and she really enjoyed having her hair and nails done Shopping was another one of her favorite things to do She had a deep love for all animals and enjoyed attending Mercy’s Bridge Church Aaron and Felicia DeWitt and Chelsea Cook; her sisters Janet Prejean and Butch and Dremeda Cook; aunt and uncles Josh and Elizabeth Thompson and Will and J.J She was preceded in death by her grandfather Funeral Services for Emily were held at 2 P.M 2024 at the Peebles West Funeral Chapel at Oakland with interment in the Morris Chapel Cemetery The family requests that memorials be directed to the Fayette County Animal Rescue To access content, please login or purchase a subscription The NetherlandsChevron North HollandChevron AmsterdamChevron How did it strike you on arrival?The spartan exterior save for a single neon side reading "and breathe" that peeks out from the green facade The good stuff: Tell us about your room.Sleek yet cozy decor peppered with calming shades of blue and dark wood furniture or shower goodies?Marie-Stella-Maris soaps and lotions and gold-plated door knockers shaped like bees while coffee table art books are stylish and entertaining Room service and food: Worth it?Downstairs the restaurant Wyers serves elevated comfort food with a Dutch twist with two patties topped with pickles and smothered in young Gouda cheese and caramelized onions for about $16 Anything stand out about other services and features?Guests are welcome to relax in the swing sets hanging in the lobby's courtyard located inside a former home that dates back to 1645 Bottom line: worth it, and why?It's the first Kimpton hotel to open in Europe, and the location is one of the most convenient in Amsterdam—just a short walk from Centraal Station. All listings featured on Condé Nast Traveler are independently selected by our editors. If you book something through our links, we may earn an affiliate commission. up-to-the-minute voice in all things travel Condé Nast Traveler is the global citizen’s bible and muse We understand that time is the greatest luxury which is why Condé Nast Traveler mines its network of experts and influencers so that you never waste a meal or a hotel stay wherever you are in the world Former Marcy Corrections Officer pleads guilty to manslaughter in case involving the death of an inmate in December 2024 — The Jamesville-DeWitt Central School District Board of Education announced the decision to select Dr Darcy Woodcock as the district’s new Superintendent of Schools The School District said Woodcock is a Central New York native with more than two decades of education experience She is currently the Superintendent of Tully Central Schools Peter Smith announced his retirement plans in October and will serve the district through the remainder of the 2024-25 school year Woodcock would begin her position with Jamesville-DeWitt on or about July 1 Woodcock served as the Assistant Superintendent for Curriculum and Instruction for the Westhill Central School District She also served as Director of Professional Development for Syracuse City Schools from 2019-2020 and as Principal of Liverpool Elementary School from 2012-2018 She also served as an assistant principal for OCM BOCES’ Regional Summer School at Fayetteville-Manlius High School from 2009-2011 Her initial teaching experiences began in Florida where she worked for three years as a primary teacher in New Port Richey she worked for eight years as a sixth-grade math and science teacher at Eagle Hill Middle School in the Fayetteville-Manlius Central School District Her educational experience includes a bachelor’s degree in elementary science education and special education from SUNY Geneseo; a master’s degree in literacy from SUNY Oswego; a Certificate of Advanced Study (CAS) from SUNY Oswego; and a doctorate in Educational Administration and Leadership from the Riley College of Education and Leadership from Walden University in Minneapolis Woodcock grew up in Central New York and graduated from Cicero-North Syracuse High School She says it’s a true honor to join Jamesville-DeWitt "I look forward to building strong relationships and earning the trust of our students I am thrilled to become part of such an outstanding school district,” she added A former Pottawatomie County Sheriff's deputy is facing legal troubles again Cleveland County prosecutors filed domestic abuse charges against retired law officer David Dewitt after a heated exchange with his wife and daughter an unwillingness from those involved to testify and a lack of forensic evidence “I knew from day one there was no case there,” said Scott Adams This time Dewitt faces domestic abuse charges the alleged victims were Dewitt's wife and daughter Police were called to the family's home in northwest Norman last month An affidavit said that Dewitt was gone when officers arrived at the home Dewitt's wife told officers they were arguing when Dewitt allegedly said he was going to shoot her and murder her Dewitt's daughter told police her father grabbed her phone to stop her from calling 911 Dewitt allegedly pushed his daughter down the stairs and then grabbed her by the neck The daughter eventually ran to a neighbor to call the police Dewitt was arrested on a warrant two weeks later Dewitt retired from law enforcement last year He is the founder and CEO of a local security company Dewitt's attorney said the wife and daughter do not want the case prosecuted Adams expected to have the domestic abuse charges dropped Jennifer Pierce is an Emmy-award-winning reporter and is a member of the Indigenous Journalists Association and general news updates from News 9 delivered right to your inbox It is with great sadness that we announce the passing of MSgt James lived a life dedicated to his country and graduated from Pasco High School before joining the United States Air Force in 1952 particularly during his time with the Precision Measurement Equipment Laboratory (PMEL) group this vital group was responsible for calibrating and certifying precision measurement equipment used by various Air Force units His work ensured the accuracy of systems related to aircraft James's dedication and expertise earned him the Bronze Star for his meritorious service as a liaison in a combat zone during the Vietnam War He voluntarily extended his time in Vietnam where his exceptional contributions in support of the mission earned him great respect from his colleagues James retired as a Master Sergeant after 22 years of service and continued his commitment to the Air Force as a civilian employee for 33 years working with the Air Superiority Missiles team as a Missile Equipment Specialist He was responsible for the BQM-34 and MQM-107 Target Drones and the FIM-92 Stinger. James was not only a dedicated serviceman but also a devoted father and grandfather lovingly regarded as a second father by his grandchildren He cherished his family deeply and took great joy in caring for his grandchildren His love for his family was matched only by his warmth and the joy he brought to everyone who knew him. often using his sharp mechanical skills to repair his own vehicles and complete DIY projects he has been a licensed ham radio operator since his youth and his ability to recall the smallest details But one thing that excelled his memory was his wit He took life a day at a time and during the most turbulent moments was always a positive force against the tide. a devoted Christian who loved Jesus and made sure his family was raised in the church always following through on his commitments and joy to all who had the privilege of knowing him. His memory will forever be treasured by his children Kurt Alan DeWitt (Kimberly) of Laguna Beach and Deirdre Anne DeWitt Josovitz of Boca Raton Karley Rutherford (Trae) and Sophie Josovitz; by his great-grandchildren and by his great-great-grandchild: Elsie Foreman Many thanks to Richard Mawson (United Kingdom) dad’s nephew on his wife Isabella’s side for Richard’s tireless pursuit to keep both continents of the extended family connected over the years Also thank you to his cousin William Myers and close friend Jerry Peacock since high school for always being by his side The family would like to extend our love and special thanks to Lisa English and her administration and physical therapy team at Putnam General Hospital (PGH) in Eatonton and direction they gave to Dad and his family They made Dad's trials less difficult and his time at PGH more memorable The cake he was able to enjoy during his 90th birthday while at PGH could only have been made possible with the loving care and next level expert care the Putnam team demonstrated A funeral service will be held at 12:00 p.m USAF (Ret.) will be laid to rest in Riverside Cemetery with military honors In lieu of flowers, the family respectfully suggests memorial donations be given in memory of James DeWitt to Disabled American Veterans at https://help.dav.org/ For friends unable to attend at this time, the service will be streamed live at https://www.youtube.com/@McCulloughFuneralHome via the McCullough Funeral Home’s YouTube page Go to www.mcculloughfh.com to sign the Online Registry for the family McCullough Funeral Home and Crematory has the privilege of being entrusted with these arrangements Make a donation to one of the following charities in remembrance of MSgt Add to Calendar 2001. Theodore was the youngest of four boys born into the loving home of Doyle and Lisa (Kinney) Fox. He died tragically in the early hours of December 12 at the age of 23. He was baptized and confirmed in the Catholic Faith at Sts Philip & James Catholic Church in Grand Mound Theodore graduated in 2020 from Central DeWitt Community High School He was proud of the work he did for Quint City Stone Center in Davenport Livestreaming at http://www.facebook.com/schultzfuneralhomes/live Theodore lived a tremendous life. Having entered into a loud chaotic family of three older brothers and countless cousins Theodore didn’t need to go far for entertainment and wrestling. Instead of adding to the raucous Theodore was often quiet and reserved. He liked being friendly and getting to know people but he wasn’t going to talk someone’s ear off. One of his greatest qualities was his openness to everyone. He didn’t care who people voted for he just accepted everyone for who they were. Along with being a loyal son Theodore was also a wonderful father to his son you would find his handwritten note with this question “How can I be of service to God today?” This was something he reflected on but his final wish would be for you to ponder it for yourself “How can I be of service to God today?” and his son’s mother Brianna Kent; nieces and nephews He is welcomed into the next life by his grandparents Philip and James Catholic Church in Grand Mound Burial will be at Elmwood Cemetery in DeWitt The family wishes to thank anyone who showed Theo loving compassion throughout his short journey with us Arrangements are in the care of Schultz Funeral Home Condolences may be expressed at www.schultzfuneralhomes.com the book tells the DeWitt County story through the eyes of German immigrants James Kearney translated a long-lost history of DeWitt County written in German by journalist C.H DeWitt County is about 90 miles east of San Antonio in a part of the state that is soaked in Texas history There are plenty of families there today related to colonists who settled when the region was a part of Mexico The county is in the midst of its 175th anniversary, and as luck would have it, a resident recently uncovered a previously unknown history of the area – just in time to celebrate the county’s history The author, C.H. Waltersdorf, immigrated to DeWitt county as a child. To unlock the manuscript’s secrets, the DeWitt County Historical Commission turned to Professor James Kearney a historian and professor of German at the University of Texas at Austin The finished product was recently unveiled to the public Kearney spoke to the Texas Standard about translating the text Listen to the interview above or read the transcript below This transcript has been edited lightly for clarity: what do we know about the person who wrote this book And so that was part of the excavation into this project that I did It was a kind of an archeology on a lot of fronts But he was a German immigrant who came to Texas in the early 1880s His family had already come before him – his father and mother left him behind as a five-year-old And eventually he started his own German newspaper “A History of DeWitt County” in German a former cheerleader from here at the University of Texas – a delightful lady named Mary Gayle DeWitt County was celebrating its 175th anniversary and she is involved in the historical commission And they decided they would like to translate it for their celebration You use the term “discovered.” How was it discovered I think she’s president of the DeWitt County Historical Society and she has her roots in the German community these people have trunks and old collections of papers and letters and she was rooting through these and she found what was published as a supplement to a newspaper There were over a hundred German language newspapers in Texas at one time She found it and she recognized “DeWitt County” in the title I realized this is actually quite an important manuscript on a lot of scores Can you just tell us what the history covers generally He starts from the very beginning about DeWitt County and Green DeWitt one of the original colonies in the Mexican period But he just covers that very perfunctorily His story really begins in depth with about 1845 And the reason is that’s when vast numbers of German immigrants began coming in and landing at Indianola DeWitt County is strategically positioned halfway between the coast And that was where all the supplies came in for so-called West Texas including these Germans who eventually became the majority population in DeWitt County So his focus is telling the story of DeWitt County from the German immigrants point of view And that’s something that’s never been done before And so it becomes a story of acculturation – of adaptation to a society and mores and things that didn’t exist in Germany and a class of people that didn’t exist Waltersdorf refers to “rowdies” or cowboys and he says this is a type of person that we’ve never encountered before I realized this is actually quite an important manuscript on a lot scores one thing immediately comes to mind – namely the Sutton-Taylor feud And there have been many books written about the Sutton-Taylor feud – many articles mention the fact that there was a large and growing immigrant community in the county that was not directly involved in the feud They’re sitting in the bleachers watching these various Anglo factions slaughter each other His book is a wonderful collection of anecdotes some of which are based on first-hand experience and some he heard you know everything that’s going on and everybody he tells the story of the famous gunslinger John Wesley Hardin and how he liked actually to hang out with the Germans in Yorktown and drink beer and go to their dances this is one of the most notorious killers in American history who had a whole other side how does it compare to contemporary German I found it delightful to translate and very easy the question is not knowing what the German says The question is finding the appropriate English equivalent of it He laced his narrative with a lot of German sayings that any German would recognize I don’t know if I can think of a precise example off the top of my head but trying to find an equivalent English expression that has the same pithiness and so forth and so on… It’s a challenge And when you feel like you’ve got something right But there’s also syntactical… You know it has a capacity to make very long sentences that is something you struggle with – how to break up these very long sentences and yet preserve a certain tone and pace throughout » GET MORE NEWS FROM AROUND THE STATE: Sign up for Texas Standard’s weekly newsletters very compelling on a lot of different levels It tells the stories of common people and the people who their energies and their efforts built the community in the county of DeWitt but they’re sort of who are left out of standard histories he tells a story about a German lady by the name of Frau Sieder who served all the wagoneers and teamsters who were driving wagons from Indianola to San Antonio and back And you’re talking about hundreds of wagons before Indianola was off the map by hurricane She would tolerate no cussing or loud behavior and everybody had to sit and eat and then plop down a quarter There’s some interesting omissions in Waltersdorf that are quite interesting and there’s never a mention of a Black person ever in the book But normally these little history books like this also tend to go into the churches and the development of the churches as an important social institution There’s not one mention ever of the religion or churches in his history of DeWitt County which is all the more interesting because briefly he got himself ordained as a German Methodist preacher and he was active for 10 years in Texas and had congregations he gave it up and his history has not one mention of religion If you found the reporting above valuable, please consider making a donation to support it here. Your gift helps pay for everything you find on texasstandard.org and KUT.org ©2024 Texas Standard. A service of the Moody College of Communication at the University of Texas at Austin | Contact us View Visitation & Service details > went to be with his Lord and Savior on March 12 He was the son of Ira and Hazel (Clark) Lowery Paul and his late wife Sara (Brubaker) served the Lord as missionaries in Guatemala for 43 years and Paul for an additional 7 years after her death His main ministry was as a professor at the Central American Theological Seminary (SETECA) in Guatemala City PA; 7 grandchildren and 2 great-grandchildren He was preceded in death by his two brothers Fred and Bob Lowery and his daughter Brenda golfing and reading were favorite pastimes He was a faithful member of Calvary Church and the Koinonia Hispanic Ministry All are invited to his Memorial Service on Thurs Mar 20 at Calvary Church (1051 Landis Valley Rd. donations can be sent by check payable to Avant Ministries Condolence Message: Be sure to include your name Please note that your condolence will not appear on this page until it is reviewed Condolences usually appear within 24 hours of being submitted My love and prayers are with you all May the Lord give you Our seven funeral homes in Lancaster and Lebanon Counties makes it easy and convenient to make arrangements and host services close to home The DeWitt girls basketball team owned one of the top backcourts in Greater Lansing this season Senior guards Tara Kurncz and Madi Uyl were fourth-year varsity players who led the charge for the Panthers in a successful winter in which they captured CAAC Blue Uyl and Kurncz were recognized for their contributions in DeWitt's 25-2 season by earning recognition on the Michigan Sports Writers all-state team in Division 1 Uyl was named to the first team and Kurncz earned second team accolades who is signed to play for Division II national champion Grand Valley State 2.4 assists and shot 48.3% from the field and 37.7% from behind the 3-point arc She finished her career with 1,058 points and and ranks fifth in program history in scoring More: Michigan Sports Writers Division 1 girls all-state basketball teams Kurncz set DeWitt's program scoring record and finished her four-year varsity career with 1,241 points She ranks second in program-history with 135 career 3-pointers Uyl and Kurncz were among four players from Greater Lansing to earn all-state recognition Holt senior Allison Metzger and East Lansing sophomore Sydney Black were selected honorable mention all-state More: Haslett, Portland have second team girls basketball all-state selections Contact Brian Calloway at bcalloway@lsj.com — Tops Markets has announced the closure of its store at 4410 E The company plans to consolidate operations into several nearby locations within a five-mile radius All employees from the DeWitt store have been offered positions at Tops locations in Fayetteville ensuring no job losses for part-time or full-time associates The Tops Xpress gas station adjacent to the DeWitt store will remain operational "This difficult decision was not taken lightly but ongoing challenges at our DeWitt location made it no longer sustainable to continue its operations we will be redirecting our focus and resources into our nearby stores," said Kathy Sautter Corporate Communications & Public Relations at Tops "We are grateful for the hard work of all our outstanding associates and we remain committed to serving the grocery needs of our communities in Central New York," Sautter added HOLT — They didn't want to be just like any other girls basketball team in DeWitt history Madi Uyl and the rest of her teammates wanted to distinguish themselves from other groups And while their season ended Tuesday with a 59-54 loss to Belleville in a Division 1 quarterfinal "They came up with 'Leave a legacy' as the motto they wanted for this year and they've done just that," DeWitt coach Bill McCullen said "They set a bunch of program best marks in a lot of different things They were great role models for these young kids that we have coming who had a chance to be part of this as well." The accomplishments for DeWitt (25-2) included a 24-game winning streak as well as winning CAAC Blue The regional crown was the first since 2019 for the Panthers who fell just short of getting to Breslin Center for the first time since 2015 when the program finished as the Class A state runner-up DeWitt also had two players — Uyl and Tara Kurncz — surpass 1,000 career points to mark the first time in program history two players reached the milestone in the same season They are among seven players in program history to reach the milestone The Panthers nearly added to those accomplishments Tuesday going toe-to-toe with Belleville (26-1) and star sophomore Sydney Savoury The game was tight for three quarters before the Tigers stretched their lead to 11 in the fourth DeWitt managed to get as close as three points on a late 3 by Uyl before having its comeback fall short "I've been doing this a long time and don't know if I've even been more proud of a singular game effort that we had against somebody," McCullen said "That's a really talented team and we traded blows with them for four quarters Even when it looked bleak and they got it to 10 or 11 points the kids kept fighting and they got it down to three and were close to getting a deflection Uyl was proud of the efforts and accomplishments by the Panthers this season and believes they achieved plenty "We've had great role models like senior classes who have done really well and were successful so I think trying to get up in the record books with them was a big push," Uyl said we just wanted to put our names in the history book "We wanted this year and this team to be memorable so I think that was the big thing We didn't want to be just one other DeWitt team that came through the program We wanted to be ones that little girls looked back to and said I want to be like that 2024-25 DeWitt team." Belleville eliminated a Greater Lansing team in a quarterfinal for a second straight season as Savoury shined with Michigan State women's basketball coach Robyn Fralick on hand watching who was named the state's Gatorade Player of the Year last week is rated the top player in the 2027 class in Michigan by Prep Girls Hoops and has attracted plenty of attention She had a stretch in which she scored 18 straight points for Belleville and then played a role in the Tigers hanging on in one of their few close games "The atmosphere was amazing and I think that kind of rattled us in the beginning," Savoury said "We handled adversity and we dealt with it and we started to play our style of basketball and getting some key stops and some key rebounds at the end." Tuesday marked just the third game decided by seven points or less this season for Belleville which got some key late free throws by Secrette Carter Belleville coach Jason Wilkins was pleased to see his team respond when pushed by DeWitt "We haven't had many close games so my kids haven't really been in that situation," Wilkins said We actually needed this and now you've got to buckle down (and) it's not about being a hero." Apply Now Schedule Sign Me Up Towering nearly 60 feet tall at the busy intersection of Wheelock and Main streets stands the stately DeWitt Elm. who was Dartmouth’s first director of facilities planning is estimated to be between 120 and 130 years old For many members of the Dartmouth community it’s a symbol of tenacity and steadfastness costly maintenance and state-of-the art treatment for Dutch elm disease experts have concluded the tree is decaying and no longer salvageable when an enormous limb that had been cabled to the trunk broke away town arborist for Hanover’s Department of Parks and Recreation He says he was somewhat surprised the limb failed since there were no outward signs of deterioration an assessment in May using tomography—sending sound waves through the wood to measure density—did find troubling signs of structural weakness in the tree It’s amazing that it survived the 1938 hurricane and the massive Dutch elm disease outbreaks in the 1940s and 1950s “There is a decay column in the bottom 50 centimeters of the trunk,” says Metcalf “We’re not concerned about a whole tree failure because the wood around the perimeter is sound But we are worried about more large limbs falling from the canopy.” poses a major risk to pedestrians and motorists The tree sits just inches from one of Hanover’s busiest intersections at the southwest corner of the Green Metcalf says he’s impressed that the tree has lasted as long as it has “It happens to be located in one of the most difficult situations for a tree to grow in with its only permeable surfaces right under the tree,” he says “It’s amazing that it survived the 1938 hurricane and the massive Dutch elm disease outbreaks in the 1940s and 1950s Work to remove the tree is expected at the end of the month or early August; traffic will be re-routed at the time and the southwest corner of the Green will be temporarily closed to accommodate the crane and chipping of branches Public notice will be given well in advance by Facilities Operations and Management He says Dartmouth and the town have worked to save elms near the Green. “We went to extreme lengths on the north side of Parkhurst to protect the big elm there we literally rerouted the sidewalk around it so that plows wouldn’t hit it and it wouldn’t get salt We’ve really tried to preserve the existing big elms.” A town memo indicates Hanover has spent almost $13,000 since 2010 to protect the DeWitt Elm with such measures as treating it for Dutch elm disease every third year and installing cables to protect and hold limbs that might fail Frank Roberts worked with DeWitt nearly 30 years ago and said he believes the tree was named for him because of his extensive work with the town when DeWitt oversaw the Facilities Planning Office “The location of the tree on the corner of the Green where the town of Hanover and the College intersect and communication that he fostered between the town and Dartmouth,” Roberts says who joined the facilities staff not long after graduating was involved in much new construction—including the Hopkins Center for the Arts—now lives in a retirement home in Woodstock He says he’s sorry that a beloved tree must come down “And here’s a story about someone I knew who lived down the street when the tree was dedicated to me and he says it will make a fine paperweight Charlotte Albright can be reached at charlotte.e.albright@dartmouth.edu We inspire students to practice good global citizenship while strengthening their own communities Act Now — As the weather is beginning to break from winter's cold Towns and villages all over Central New York are dealing with the ditches You can find them on busy intersections or in parking lots Bernard Alex said the town takes care of the neighborhoods in the Dewitt community where he says no complaints have been made Alex encourages people to call if you see problem spots but make sure you’re reaching out to the right people main highways like pothole-laden Erie Boulevard are taken care of by the state "People will generally say Dewitt needs to do better or Syracuse needs to do it," Alex said "You may think it's Dewitt’s responsibility but those roads are maintained and they are taken care of and they are paved by New York State." CNY Central is criss-crossing Central New York this month in search of bad potholes and what local or state work crews are doing about them send CNY Central an email at news@cnycentral.com Please sign in to access the full marsh.com site Click here to sign in as colleague We bring an unmatched combination of industry specific expertise and global experience to the range of risks you face and claims advocacy services leverage data and analytics to help you better quantify and manage risk and guidance you need to better understand today’s world of increasing risk and complexity — and find the opportunity in it We empower our clients to build the confidence to thrive through the power of perspective — uniting deep expertise innovative solutions and a legacy built over 150 years Sarah served as the head of Financial Planning and Analysis and Investor Relations at Marsh McLennan She joined the company in 2019 as head of Investor Relations she also assumed responsibility for FP&A Sarah was responsible for the firm’s financial forecasting as well as investor communications (including interactions with shareholders and other financial stakeholders) and the creation and dissemination of external financial communications Sarah was ranked a top Investor Relations Professional from 2020 to 2023 in the Institutional Investors’ All-America Executive Team rankings property/casualty insurance equity research analyst at J.P Sarah has 15 years of experience covering the property/casualty insurance industry as an equity analyst she covered the industry at Barclays and Lehman Brothers Sarah earned her BA in economics and BS in finance from Providence College in 2003 helping clients navigate a dynamic environment through four global businesses she was the cherished daughter of the late Glyndon and Kathleen Taylor Simmers. Glenda had a deep love for the simple pleasures in life - whether it was getting lost in the pages of a good book or reminiscing about her favorite television shows and her favorite classic TV shows were Gunsmoke and Bonanza. She was a woman of deep compassion and joy finding solace in the companionship of her beloved dog a reminder of nature’s small wonders that brought her so much joy and peace One of her greatest joys was her love for her daughter’s horses Not only did she help care for them with a tenderness and concern that reflected her deep affection for these beautiful creatures but she also observed them with a sense of childlike wonder Whether watching them gallop across the field or marveling at their gentle nature Glenda’s eyes would light up with excitement when talking about them as if she were seeing the world through a fresh The horses held a special place in her heart and she took great delight in their company sharing her enthusiasm with anyone who would listen. with her garden thriving under her loving care She took great pride in nurturing life in all its forms. Glenda was also an active and proud member of the Eagles Club and the Moose Lodge where she touched many lives with her gentle spirit and infectious warmth. But perhaps the most profound legacy Glenda leaves behind is the love she gave so freely - especially to her grandchildren and unwavering love will live on in them and in the countless hearts she touched Her children find comfort in knowing that their mother has finally been reunited with her own mother She is now cradled in their loving arms. Glenda is survived by her devoted daughters Alicia Midkiff and Denise Welcher; her aunt Stephanie Wallace; and her special friends whose friendships meant the world to her. Carl “Butch” Simmers and Kenneth Simmers; her nephew A Celebration of Life will be held at 2:00 PM on Saturday A graveside service will follow at Augusta Memorial Park in Waynesboro. memorial contributions may be made to the Augusta Health Hospice of the Shenandoah (c/o Augusta Health Foundation VA 22939) or to the Friends of the Shenandoah Animal Services Center (104 Lanchester Court a testament to the love and care that defined Glenda’s journey here on earth DEWITT — An annual electric light parade that most everyone agrees was a success has sparked turmoil in the city after a complaint about how police handled traffic control More than a month after the parade in early December the parade will no longer be hosted by the DeWitt Area Chamber of Commerce and the city has yanked its membership from the business organization The dispute began after Chamber President Loretta Spinrad complained to city officials about the police department’s traffic control decisions Spinrad explained that she was upset when Police Chief Bryan Curtis didn't put up “no parking” signs in the staging area and people drove around barricades “A couple of kids almost got hit,” she said I have been told that the council never hears the downside of anything both the DeWitt City Council and DeWitt Downtown Development Authority voted to “withdraw” from the chamber traditionally known for being a nonprofit organization and consortium of about 60 local businesses Council voted unanimously to withdraw their membership on Jan 13 “due to untrue and unprofessional comments by the DeWitt Area Chamber of Commerce,” according to meeting minutes The DDA’s board followed the council's lead there were some comments made from board members of the chamber of commerce directly to myself and to city council,” said Dan Coss the city’s administrator and DDA’s executive director it was just best to go in a different direction and not continue to be members with that organization “They made some very disparaging remarks about our chief and our police department and police officers as it related to traffic control and the way they performed their duties.” Coss said city officials wanted to avoid a situation of “he said she said,” and so “we just decided that as a municipality the direction leadership was going just wasn’t for us and wasn’t consistent with the way we operate." He emphasized that “for sure” the electric light parade will be back for the holidays but that the DDA or another community group may assume responsibility for the event "Thank you for the opportunity - much appreciated - however I think Dan covered it," she said in an email referencing Coss who joined the DeWitt Police Department a few years ago after serving with the Lansing Police Department and working decades in law enforcement said traffic control wasn't perfect but was reasonable He was appointed interim chief in October and was a few days shy of being appointed the new chief when DeWitt’s Christmas Market and Electric Light Parade happened Curtis said there were parking signs near the staging area it seemed like there was enough room and he didn’t want to call in tow trucks during the event requiring someone to go through a barricade He and Spinrad have talked to try to resolve their differences I did everything in our power to make sure that the festival ran smoothly and it did A lot of people enjoyed themselves that evening.” While Coss said the city is willing to still work with the chamber Spinrad has concerns about the chamber’s future “We hold a free concert series in the park We don’t know if we’re going to be able to do that We hold a large Halloween event in the park We don’t know if we’re going to be allowed to do that now They just feel that we’re in their way or something.” Spinrad said what has happened seems right out of sixth grade who is also a retired Cooley Law School instructor said the drama sounds like a personality conflict and the withdrawal from the chamber because of a dispute a rarity and that brings the parties closer together when they meet and figure it out so this is a little surprising,” he said Contact editor Susan Vela at svela@lsj.com or 248-873-7044