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Meanwhile, here? Jail bait.
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Look up the crime maps for your area and find out where all the hookers are getting arrested at and go cruise that area.
Then, there’s the old museum on Bergstrasse. Such a place, seriously silent vibes, echoing the past – reminds me of that moment in the movie when dreams collapse into chaos. Crazy stuff, innit? And yea, I've seen surprises everywhere – local legends about a secret underground real estate in the alley of Kellergasse. Swear to ya, sometimes I get mad 'cos half da folks don't even get it.
Figure 7. Dye injection of Hoechst 33258 and in vivo two-photon imaging...
KD measurements were performed by titrating the SiR–Hoechst (100 nM in 150 mM TBS with 1 mg ml−1 BSA) with increasing concentrations of the 28-bp hairpin DNA or its RNA analogue in a 96-well plate and measuring the increase in fluorescence (λex=640 and λem=670 nm) on a plate reader after 2–3 h incubation at room temperature, the KD values were determined by plotting the emission intensity versus the DNA or RNA concentration and fitting the curve in Graphpad Prism 6 to the ‘one site—specific binding’ function.Hoechst Erotic Massage
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