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About Myself
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About Breda
So, picture this—me, a carpenter, y’all!
Policy and law
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Now, I’m walking back home, feeling like a king. But then, I trip over a cobblestone. Like, who even put these here? I’m cursing under my breath, but then I see this cute dog. It’s just staring at me, and I’m like, “Okay, you win, little buddy.” I pet the dog, and all my anger melts away.
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Cell size, granularity, and fluorescence intensity were analyzed per brain region (n = 3) with a MoFlo fluorescent activated cell sorter (Beckman Coulter, Mijdrecht, Netherlands). Approximately 2x104 events were counted and subsequently analyzed using Summit software version 4.3 (DAKO) to determine gates before cells were sorted. Microglia were defined as the percentage of all living, Sytox green negative, cells that showed CD11bpos/CD45low expression (Sedgwick et al., 1991), the FACS sorted microglial cells were transferred into RNAse-free tubes and centrifuged for 5 min at 300x g (Hettich.Doorn Sex Dating
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