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Henrietta, N.Y. — The smooth sounds of jazz took over Lovin' Cup at Park Point.
World-renowned contemporary jazz band Cabo Frio celebrated 45 years of music success with a performance Thursday night.
The band chose to perform in the Rochester area, the place of their humble beginnings.
Cabo Frio band leader Curtis Kendrick says it's all because of the fans, and the band looks forward to celebrating now and for many years to come.
ExplorationPetrobras drills fresh well at Brazil mega pre-salt blockBrazilian oil giant working to delineate oil find in Alto de Cabo Frio Central block
September 16, 2023JPEG
When European explorers first surveyed the coastline of what is now the state of Rio de Janeiro in the early 1500s
and lush green mountains rising from the sea
were unusually cool—so much so that the promontory in southeastern Brazil shown above was named Cabo Frio
The upwelling is primarily caused by the prevailing winds. Strong northeasterly winds that blow along the coast for much of the year—especially in the spring and summer—push warm surface waters away from the shore. This allows cool waters from deeper in the ocean to rise to the surface. Upwelled water is typically rich in nutrients that fuel populations of phytoplankton
which in turn nourish marine ecosystems and contribute to Cabo Frio’s productive fisheries
NASA Earth Observatory images by Lauren Dauphin, using Landsat data from the U.S. Geological Survey and data from the Multiscale Ultrahigh Resolution (MUR) project. Story by Adam Voiland
View this area in EO Explorer
People have long noticed that the waters around Cabo Frio are unusually cool
The signs of economic activity around this Brazilian city are easy to see from space
Unusually cloud-free skies allowed satellites to observe colorful phenomenon taking shape in the region’s coastal waters
cool waters and abundant sunlight provoked a widespread phytoplankton bloom
Metrics details
The quantitative assessment of the carbonate system represents one of the biggest challenges toward the "Sustainable Development Goals" defined by the United Nations in 2015
the present study investigated the Spatio-temporal dynamics of the carbonate system and the effects of the El Niño and La Niña phenomena over the Cabo Frio upwelling area
The physical characterization of the site was carried out through data on wind speed and sea surface temperature
Water samples were also collected during the oceanographic cruise onboard the Diadorim R/V (Research Vessel)
the parameters of absolute and practical salinity
The highest average concentration of bicarbonate in S1 (2018 µmol/kg) seems to contribute to the dissolved inorganic carbon values (2203 µmol/kg)
and carbonate were higher on the surface of each station (calcite saturation state = 4.80–5.48; aragonite saturation state = 3.10–3.63
The mean values of pH were similar in the day/night samples (7.96/7.97)
The whole carbonate system was calculated through thermodynamic modeling with the Marine Chemical Analysis (AQM) program loaded with the results of the following parameters: temperature
This manuscript presents original data on the carbonate system and the "acidification" process influenced by the Cabo Frio upwelling
which directly depends on the El Niño and La Niña phenomena oscillations in the sea surface temperature
This is conditioned to the power of events that affect the tropical and subtropical cyclone and anticyclone systems
changing the intensity of the winds nearby the upwelling region and leading to an increase in the SST
The influence of these phenomena in the Cabo Frio upwelling region has not yet been fully understood
nor have their implications for the carbonate saturation state
This experiment is part of an extensive study on the feasibility of implementing a unified protocol in a monitoring program for the acidification of coastal and offshore areas of the Brazilian ocean
The present investigation originally elucidates the space–time dynamics of the carbonate system in the Cabo Frio resurgence and assesses La Niña and El Niño in the resurgence phenomenon
Study area. Sampling stations are represented by dots and respective numbers (S1, S2, S3, and S4). This map was generated with the ArcMap software v. 10.8.2 (https://www.esri.com/en-us/arcgis/about-arcgis/overview)
The authors found that when the SASA shifts poleward
the SAM was in a positive phase of La Niña
the SAM was in a negative phase during El Niño
The wind speeds observed across Brazil can also describe this shift in the SASA pattern
The data series of SST weekly averages referred to the period between 1994 and 2016 (data from the Admiral Paulo Moreira Marine Research Institute—IEAPM)
Trend curves were also obtained relating the average wind velocity with the SST
Pearson's R2 coefficient was used to assess data correlation
The sampling campaign involved a two-scale analysis. The spatial scale was held perpendicular to the coastline (S1–S4), Fig. 1
The ship was anchored in station 12 for 12 h for the temporal scale
Both campaigns were performed on the same day
water was sampled from the surface (~ 3 m) between 6:30 and 10:00 h (UTC) using a pump adapted to a hose without forming bubbles
The other water samples below the surface were collected through a Niskin bottle of 10 L for the middle (half of the total depth) and bottom (~ 5 m above the seafloor)
the surface water samples were collected hourly (12 samples total)
while middle and bottom water samples were collected in alternate hours: 13
were performed with a CTD vessel (Midas Valeport)
The AQM is a package of thermodynamic equations
which can predict the complex composition of the marine carbonate system
This package is based on measurements that can be relatively inexpensively (pH
reducing the overall costs of ocean acidification monitoring programs
The AQM program is available upon request to the corresponding author’s email
Non-parametric Kruskal Wallis test was chosen for comparisons between groups
All statistical tests were performed using the Statistica 7.0 software (TIBCO) with a significance level set at p < 0.05
A Thermo Scientific Orion Star potentiometer coupled to the Orion glass reference electrode cell
model 8102BNUWP was used for potentiometric determinations
The pH electrode was calibrated daily with "Tris" buffer (0.04 m) for sample readings (maximum 12 samples per day)
Due to the reduced number of samples per day
the short period of the oceanographic cruise
and the constant working conditions (electricity source
we chose to verify the electrode performance at the beginning and the end of the oceanographic cruise
The electrode's percent efficiency ranged between 99.49 and 99.54% concerning the theoretical Nernst value (59 mV)
More details are available in hydrogen potential (pH)
The normalized total alkalinity (NTA) was obtained by the AQM program using the equation: NTA (µmol/kg) = TA (µmol/kg) × 35/Salinity (g/kg)
where 35 was assumed to be the representative salinity of the water masses
The total pH of the water samples collected during the cruise was determined in the "wet laboratory" as follows: pHT (= − log([H+] + [(HSO4−]/co)
where co is the thermodynamic concentration (1 mol/kg-soln)
The internal solution of the combined pH electrode was filled up with 0.7 m NaCl to reduce the potential liquid junction. The electrode's electromotive force (emf) was related to the molar concentration of the proton [H+], as shown in Eq. (2)
\({lnk}_{B}^{*}\)53
\({lnk}_{Si}^{*}\)54
\({lnk}_{1}^{*}\)(H3PO4)55
\({lnk}_{2}^{*}\) (\({H}_{2}{PO}_{4}^{-}\))55
\({lnk}_{3}^{*}\) (\({HPO}_{4}^{2-}\))55
\({lnk}_{2}^{*}\) (\({CO}_{3}^{2-}\))56
The CO2 flux equation between oceans and the atmosphere is defined between aqueous CO2 and saturated CO2, defined as follows (Eq. 3):
Temperature of the longitudinal samples from the surface, middle, and bottom of the water column.
Salinity of the longitudinal samples from the surface, middle, and bottom of the water column.
Dissolved oxygen in the longitudinal samples from the surface
Wind velocity at quadrants SW and NE (blue line) with the respective variation of sea surface temperature (SST) at the Cabo Frio/RJ upwelling area (circle with a vertical bar in red), showing the average temperature and the standard deviation between 09/2006 and 12/2016, according to mild (Weak), and moderate to strong (Mod. to Str.) El Niño and La Niña events.
Temperature Grid of the Sea Surface Temperature (SST) at the Cabo Frio upwelling obtained by Kriging the SST data (1995–2016)
Blue spots (cold): longer-term and intensity of the upwelling phenomenon
Red blots (hot): longer-term and intensity of temperature anomalies
Ellipse: SST in the period correspondent to the sampling campaign
Hachured rectangle: Str—increased wind magnitude period (from 2010 to 2016); Weak—absence of the events of a mild El Niño (from 2012 to 2013); and Mod
To Str—moderate to strong winds (from 2006 to 2010)
but on a larger time scale as the El Niño periods
considering stronger winds responsible for changes in the resurgence process pattern
generating reflexes in the resurgence process local
The author also found that the Cabo Frio resurgence phenomenon is related to the frequency of the El Niño and La Niña events
causing changes in SST and the wind dynamics throughout the seasons
affecting the nutrient transport and dispersion from the sea bottom to the surface in the upwelling vicinity
Quantile–Quantile plot (QQ-plot) displaying the SST and Wind Velocity data distribution from 2006 to 2016 in the Cabo Frio upwelling area
Greenline: NE wind intensity trend curve; blue line: SW wind intensity trend curve
pH and Alkalinity of the longitudinal samples from the surface
Calcite saturation state (ΩCalcite) and carbonate concentration (µmol/kg) of the longitudinal samples from the water column's surface
characterizing the effects of the intense El Niño on the Cabo Frio upwelling system
Temporal sampling. Temperature (°C), salinity (psu), aragonite, and calcite saturation state. X-axis: hour–day. Y-axis: coordinates of stations. Z-axis: Depth-m. Data sampled from 1:00 p.m. to 1:00 a.m. on the 20th and 21st of January 2016.
Dissolved Oxygen (µmol/kg) and Carbon Dioxide Partial Pressure (atm)
All these authors suggested the corrosive effect of upwelled waters (pH < 7.75 and ΩAr < 1.0)
The El Niño Southern Oscillation might affect the upwelling intensity by raising the SST
This climate phenomenon influences the carbonate system since these parameters are affected by SACW
Calcium carbonate seems responsible for the increased values of TA in all studied stations
DIC was influenced by bicarbonate concentrations from upwelled waters
the effect of sunlight on the carbonate system parameters over 12 h was not observed
the results of our study emphasize the importance of ENOS phenomena
and the nodal cycle should be considered in studies of acidification of the oceans in resurgence areas
Interdisciplinary studies with the implementation of a specific protocol for temporal and seasonal scales are necessary to understand the effects on biota and support climate change models
The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request
Inventory of water masses and carbonate system from Brazilian’s northeast coast: Monitoring ocean acidification
Variability and transport of inorganic carbon dioxide in a tropical estuary
Chester, R. Marine Geochemistry (Academic Division of Unwin Hyman Ltd, 1990). https://doi.org/10.1007/978-94-010-9488-7
Acidification of subsurface coastal waters enhanced by eutrophication
Evidence for upwelling of corrosive ‘acidified’ water onto the continental shelf
Fassbender, A. J., Sabine, C. L. & Feifel, K. M. Consideration of coastal carbonate chemistry in understanding biological calcification. Geophys. Res. Lett. https://doi.org/10.1002/2016GL068860 (2016)
Predominance of heavily calcified coccolithophores at low CaCO3 saturation during winter in the Bay of Biscay
Hofmann, G. E. et al. The effect of Ocean acidification on calcifying organisms in marine ecosystems: An organism-to-ecosystem perspective. Annu. Rev. Ecol. Evol. Syst. https://doi.org/10.1146/annurev.ecolsys.110308.120227 (2010)
Watson, S. A. Giant clams and rising CO2: Light may ameliorate effects of ocean acidification on a solar-powered animal. PLoS ONE https://doi.org/10.1371/journal.pone.0128405 (2015)
United Nations Summit on Sustainable Development 2015: Informal summary
in 70th Session of the General Assembly 1–10 (2015)
Data management strategy to improve global use of ocean acidification data and information
Core principles of the California current acidification network: Linking chemistry
Acidificação dos oceanos em um sopro: Prática educacional para construção de conhecimento das mudanças globais
The impact of organic and intensive farming on the tropical estuary
Lübbecke, J. F., Burls, N. J., Reason, C. J. C. & Mcphaden, M. J. Variability in the South Atlantic anticyclone and the Atlantic Niño mode. J. Clim. https://doi.org/10.1175/JCLI-D-14-00202.1 (2014)
Estudo Das Massas D’água e da Circulação Geostrófica na Região Sudeste da Bacia Do Brasil
COPPE/UFRJ (Universidade Federal do Rio de Janeiro
Valentin, J. L. Analyse des paramètres hydrobiologiques dans la remontée de Cabo Frio (Brésil). Mar. Biol. https://doi.org/10.1007/BF00392407 (1984)
Dereczynski, C. P. & Menezes, W. F. Meteorologia da bacia de campos. in Meteorologia e Oceanografia 1–54 (Elsevier, 2015). https://doi.org/10.1016/B978-85-352-6208-7.50008-8
The South Atlantic subtropical anticyclone: Present and future climate
Position of the South Atlantic anticyclone and its impact on surface conditions across Brazil
OCO-2 advances photosynthesis observation from space via solar-induced chlorophyll fluorescence
Trends in the Southern Annular Mode from observations and reanalyses
Niño/Southern Oscillation behaviour since 1871 as diagnosed in an extended multivariate ENSO index (MEI.ext)
A corrente do Brasil ao largo da costa leste brasileira
The shelf and coastal waters off southern Brazil
Negative ocean-atmosphere feedback in the South Atlantic Convergence Zone
Variação sazonal da estrutura de massas de água na plataforma continental do Amazonas e área oceânica adjacente
On the water masses and mean circulation of the South Atlantic Ocean
Shelf break upwelling driven by Brazil Current Cyclonic Meanders
Estimativa da produção primária e biomassa fitoplanctônica através de sensoriamento remoto da cor do oceano e dados in situ na costa sudeste brasileira
Physical oceanography of the western Atlantic continental shelf located between 4 N and 34 S
Ritmo climático e extração de sal em Cabo Frio
dois microclimas distintos a um curto intervalo espacial
Isaaks, E. H. & Srivastava, R. M. Spatial continuity measures for probabilistic and deterministic geostatistics. Math. Geol. https://doi.org/10.1007/BF00892982 (1988)
Methods for statistical data analysis of multivariate observations
NOAA. GeoPlatform. NOAA Climate. https://noaa.maps.arcgis.com/home/group.html?id=b4e31886df6740b68f35953cfe15079f#overview (2019)
Handbook of methods for the analysis of the various parameters of the carbon dioxide system in sea water; version 2
Measurement of pHT values of Tris buffers in artificial seawater at varying mole ratios of Tris: Tris·HCl
Determination of alkalinities of estuarine waters by a two-point potentiometric titration
Reference material for oceanic CO2 measurements Batch 104 (Bottled on June 11
Hunter, K. A. The temperature dependence of pH in surface seawater. Deep. Res. Part I Oceanogr. Res. Pap. https://doi.org/10.1016/S0967-0637(98)00047-8 (1998)
Solubility of siderite (FeCO3) in NaCl solutions
A precise and rapid analytical procedure for alkalinity determination
Millero, F. J. et al. The use of buffers to measure the pH of seawater. Mar. Chem. https://doi.org/10.1016/0304-4203(93)90199-X (1993)
Clayton, T. D. & Byrne, R. H. Spectrophotometric seawater pH measurements: total hydrogen ion concentration scale calibration of m-cresol purple and at-sea results. Deep. Res. Part I. https://doi.org/10.1016/0967-0637(93)90048-8 (1993)
Thermodynamics of the carbon dioxide system in the oceans
Ocean pCO2 calculated from dissolved inorganic carbon
and equations for K1 and K2: Validation based on laboratory measurements of CO2 in gas and seawater at equilibrium
Thermodynamics of the dissociation of boric acid in synthetic seawater from 273.15 to 318.15 K
The chemistry of the anoxic waters in the Framvaren Fjord
The dissociation constants of carbonic acid in seawater at salinities 5 to 45 and temperatures 0 to 45 °C
Dissociation constants of carbonic acid in seawater as a function of salinity and temperature
On the parameters influencing air–water gas exchange
Measurement of the diffusion coefficients of sparingly soluble gases in water
Relationship between wind speed and gas exchange over the ocean
Variabilidade Interanual da Ressurgência de Cabo Frio
UFRJ (Universidade Federal do Rio de Janeiro
Impact of the astronomical lunar 18.6-y tidal cycle on El-Niño and Southern Oscillation
Integrating hierarchical bioavailability and population distribution into potential eco-risk assessment of heavy metals in road dust: A case study in Xiandao District
Aragonite saturation state in a monsoonal upwelling system off Java
Biophysical interactions in the cabo frio upwelling system
Guenther, M. et al. Plankton trophic structure and particulate organic carbon production during a coastal downwelling-upwelling cycle. Mar. Ecol. Prog. Ser. https://doi.org/10.3354/meps07458 (2008)
Allemand, D., Tambutté, É., Zoccola, D. & Tambutté, S. Coral calcification, cells to reefs. in Coral Reefs: An Ecosystem in Transition 119–150 (Springer Netherlands, 2011). https://doi.org/10.1007/978-94-007-0114-4_9
The Omega myth: What really drives lower calcification rates in an acidifying ocean
Proton channels in algae: Reasons to be excited
Ocean acidification as a multiple driver: How interactions between changing seawater carbonate parameters affect marine life
Calcium carbonate saturation state: On myths and this or that stories
An inter-laboratory comparison assessing the quality of seawater carbon dioxide measurements
Component-specific dynamics of riverine mangrove CO2 efflux in the Florida coastal Everglades
Seasonal variability of the effect of coral reefs on seawater CO2 and air-sea CO2Exchange
The partial pressure of carbon dioxide and air–sea fluxes in the northern South China Sea in spring
On the seasonal variation of air–sea CO2 fluxes in the outer Changjiang (Yangtze River) Estuary
Variability of the gas transfer velocity of CO2 in a macrotidal estuary (the Scheldt)
Observations of pCO2 in the coastal upwelling off Chile: Spatial and temporal extrapolation using satellite data
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Volume 9 - 2022 | https://doi.org/10.3389/fmars.2022.867554
Symbiotic scyphozoan jellyfish are poorly understood in terms of their symbionts and traits
as well as the ecological significance of this association
Dinoflagellate symbionts of the medusae Cotylorhiza tuberculata
and Cassiopea xamachana collected in the Mediterranean Sea and Cabo Frio (Rio de Janeiro
Brazil) were phylogenetically identified based on 28S rDNA and ITS2 haplotypes
The studied medusae harbour only one phylotype of symbionts in a time
but scyphozoan jellyfishes can associate with several types of symbionts
This study confirmed that the main symbionts of investigated scyphozoans belong to the genera Symbiodinium
The associations between dinoflagellate symbionts and Cotylorhiza tuberculata changed from year to year
hosting Philozoon one year and Breviolum another
Invasive species in the Mediterranean Sea Phyllorhiza punctata harboured dinoflagellate symbionts of genus Symbiodinium as in the native areal
Pigment analysis of two shallow-water symbiont species Breviolum sp
and Philozoon medusarum revealed characteristic profiles for each genus
horizontally transmitted symbionts are translocated into the host in each generation
we used a phylogenetic approach using nuclear 28S rDNA and ITS2 markers to identify symbionts in Cotylorhiza tuberculata and Phyllorhiza punctata from the Mediterranean Sea during blooming period and Cassiopea xamachana collected at Cabo Frio (Rio de Janeiro
symbionts were isolated from Cotylorhiza tuberculata and Cassiopea xamachana and cultivated symbiotic cells were used in cloning experiment to reveal the diversity of the ITS2 region of the dinoflagellate cells within individual host medusa
Photosynthetic pigments of two different genera of symbionts from Cotylorhiza tuberculata were characterised
Symbionts were isolated from live medusae by scraping cells from the subumbrella and oral arms immediately after collection
Part of sample was stored for pigment analysis and another part was stored in cryotubes in 96% ethanol and kept at -80°C for DNA extraction
Figure 1 Sampling sites of Cotylorhiza tuberculata and Phylorhiza punctata in the Mediterranean Sea and the sampling site of Cassiopea xamachana from the coast of Cabo Frio (Rio de Janeiro
washing of about 200 to 300 cells with fresh sterile medium was continued twice a day for three days
The strains grew best between 20°C and 25°C with a 12 h/12 h light/dark cycle
28S rDNA and ITS2 markers were amplified from the cultures of the symbionts and used for phylogenetic analysis
DNA was extracted from scrapings of symbionts living in the oral arms and subumbrella of Cotylorhiza tuberculata
Phyllorhiza punctata and Cassiopea xamachana
as well as from cultured symbionts isolated from Cotylorhiza tuberculata and Cassiopea xamachana
USA) was used for DNA extraction according to the protocol
The symbiont cells removed from the host were stored at -80°C
thawed on ice and ethanol evaporated in a vacuum concentrator before DNA extraction
The 28S rDNA of the symbionts was amplified with dinoflagellate-specific primers (28Forward: 5’- CCC GCTGAATTTAAGCATATAAGTAAGCGG -3’ and 28Reverse: 5’- GTTAGACTCCTTGGTCCGTGT TTCAAGA -3’) designed by Zardoya and colleagues (Zardoya et al., 1995) at position 26 onward
and reverse primers at position 741 containing the variable domain D1 and D2
The length of the amplified 28S rDNA fragments was approximately 630 base pairs
The major components of the PCR mixture were added at the following concentrations: 0.625 unit TopTaq polymerase (Qiagen)
0.05 µg µL-1 bovine serum albumin
The thermal profile included an initial denaturation at 94°C and an annealing temperature of 57°C for a total of 30 amplification cycles
Due to the low amplification efficiency of some samples
re-amplification with a further 25 cycles at an annealing temperature of 60°C was required; 5 µL of the PCR products were used as template
and the final reagent concentrations were the same as for the previous PCR
Amplification of ITS2 from symbionts was performed using a dinoflagellate-specific primer (ITSintfor2 5’ GAATTGCAGAACTCCGTG-3’), which annealed to a conserved region of the 5.8S rDNA, and the Chlorophyta-specific reverse primer (ITSreverse 5’- GGGATCCATA TGCTTAAGTTCAGCGGGT -3), as described by LaJeunesse (2002)
The length of the amplified fragments was between 300 and 330 base pairs
The optimal concentrations for PCR were 0.625 units of GoTaq polymerase (Promega
and up to 10 ng of DNA in a volume of 20 µL
The thermal profile of the touch-up PCR began with an initial denaturation step
followed by annealing at a starting temperature of 52°C for 40 seconds
with subsequent annealing at this temperature for a further 20 cycles
5 µL of the PCR mixture was transferred to a new tube
to which the PCR reagents were added at the same concentration as the first PCR and amplified at an annealing temperature of 54°C for a further 30 cycles
Sanger sequencing was performed at the commercial supplier Macrogen (The Netherlands) using the same primer pairs as the PCR
Our aim was to reveal the diversity of the ITS2 region within the ribosomal operon of the symbiont harboured by Cotylorhiza tuberculata collected in November 2009 from the Mar Menor Lagoon (Balearic Sea) to verify mixed or homogeneous infection by symbionts in individual medusa
DNA extraction and ITS2 amplification were performed as described above
The PCR product (fragment length 330 base pairs) was cloned into chemically competent One Shot Top 10 cells from the TA Cloning Kit (Invitrogen
USA) according to the manufacturer’s instructions
A total of 273 transformants were screened
of which 184 white transformants were transferred to Luria-Bertani medium with 10% glycerol and allowed to grow overnight before plasmid extraction and Sanger sequencing
Samples of the isolated endosymbionts were extracted by sonication in 90% acetone and centrifuged at 4 000 rpm for 10 min to remove particles
A mixture (1:1) of clarified extract and 1 mol L-1 ammonium acetate was injected into the HPLC system (1260 Infinity
Agilent Technologies) equipped with a 3 µm C18 reversed-phase column (Pecosphere
Perkin Elmer) to determine the composition of photosynthetic pigments (chlorophylls and carotenoids) in the endosymbionts
Chlorophylls and carotenoids were detected by absorbance at 440 nm using a Diode Array Detector (DAD; Agilent Technologies
Strains T1 and T5 are available in the Collection of Sea Microorganisms (CoSMi) at the Istituto Nazionale di Oceanografia e di Geofisica Sperimentale under strain numbers 1062 and 1065
Figure 2 Bayesian inference based on 28S rDNA sequences of symbionts from Cotylorhiza tuberculata
and Cassiopea xamachana under the TIM3+I+G model
Numbers at the nodes indicate posterior probabilities
and sampling site; sequences from this study are in bold
The ITS2 sequences of symbionts from Cotylorhiza tuberculata samples collected in the Vlicho Bay
Lefkada (Ionian Sea) and the Adriatic Sea (Gulf of Trieste and Lake Mljet) belong to Philozoon medusarum and the Breviolum group
symbionts collected in samples of Cotylorhiza tuberculata from the Mar Menor pertain only to the Breviolum group
Several symbionts were found in medusae of Cotylorhiza tuberculata from the Gulf of Trieste
the most common being members of genus Breviolum (type B2)
though members of genus Philozoon were also found in 2010 and 2011
Symbionts from Phyllorhiza punctata (KP015090 and KP015089) collected in the Ionian Sea were identified as Symbiodinium sp
The symbionts of Cassiopea xamachana were identified as Breviolum sp.
We found that Philozoon medusarum forms a symbiosis with Cotylorhiza tuberculata and anthozoan Cladocora caespitosa (MG991827
Members of this large group can form symbioses with various hosts around the Mediterranean Sea
The same group also includes the ITS2 sequences of symbionts from the anthozoan Paranemonia cinerea (Contarini
1775) from the Balearic Sea and the Algerian Basin
Table 1 Contribution of four different pigments (chlorophyll c2
and β,β-carotene) with and without chlorophyll a
This means that we could overlooked other types of symbionts
we would like to emphasise that the identity of isolated strains and symbionts taken from the host prior the cultivation were confirmed by phylogenetic analysis during study
But no distinctive features at light microscopic level could be found for the isolated strains
For additional morphological information a careful examination at ultrastructural level (SEM) of the different clades and species is indicated as an important completion of the genetic
The optimisation of photosynthesis is a clear example of these constraints
The dilemma between optimal light exposure and low UV radiation requires balancing and adaptations by both partners
their high metabolic activity and their sinuous folding
This study also reveal that the endosymbionts revealed no significant differences in size neither chlorophyll a concentration within the oral arms and the umbrella
Figure 3 Contribution of five different pigments (chlorophyll a
ββ car) in the symbionts identified as Breviolum sp
Balearic Sea; October 2010) and Philozoon medusarum (Gulf of Trieste
August 2011) isolated from Cotylorhiza tuberculata
Symbionts in medusae of Cotylorhiza tuberculata
and Cassiopea xamachana collected in the Mediterranean Sea and from the coast of Cabo Frio (Rio de Janeiro
Brazil) were identified by haplotypes of 28S rDNA and ITS2
We confirmed that the predominant symbionts in the investigated scyphozoan jellyfishes Cotylorhiza tuberculata belong to genera Breviolum and Philozoon
while non-indigenous species Phyllorhiza punctata harbour Symbiodinium
Cassiopea xamachana hosted Breviolum and Cladocopium symbiotic dinoflagellates
The individual medusae studied harbour only one phylotype of symbionts at a time as confirmed with detailed analysis of the ITS2 region within the ribosomal operon of the symbiont
although symbionts in zooxanthellate jellyfish can be different between years as we confirmed in Cotylorhiza tuberculata from the eastern Mediterranean Sea
The population of medusae from Mar Menor hosted only symbionts of Breviolum
while Breviolum and Philozoon were present in medusae from the Adriatic Sea
the possession of zooxanthellae is a unique feature of scyphozoans jellyfish that allows them to occupy several niches compared to non-zooxanthellate species due to mixotrophic way of nutrition
Haplotypes from this study are deposited in GeneBank under accession numbers listed in Tables S2, S3 in the Supplementary Material
All authors contributed to the article and approved the submitted version
Flander-Putrle was funded by the ARRS Research Program P1-0237 (Marine Coastal Research) and by the bilateral cooperation between Slovenia and Brazil (agreements BI-BR /10-12-005) and project RI-SI-2 LifeWatch financed by Ministry of Education
Science and Sport of Slovenia and the European Regional Development Fund
Raspor Dall’Olio was funded by the ARRS grant for young researchers (MR -33223)
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations
Any product that may be evaluated in this article
or claim that may be made by its manufacturer
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who kindly provided samples for this study
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Morphologic and Genetic Variability in the Marine Planktonic Ciliate Laboea Strobila Lohmann 1908 (Ciliophora
doi: 10.1111/j.1550-7408.2004.tb00567.x
Ecological Aspects of Early Life Stages of Cotylorhiza Tuberculata (Scyphozoa: Rhizostomae) Affecting its Pelagic Population Success
CrossRef Full Text | Google Scholar
Flexibility and Specificity in Coral-Algal Symbiosis: Diversity
doi: 10.1146/annurev.ecolsys.34.011802.132417
CrossRef Full Text | Google Scholar
Molecular Diversity of Dinoflagellate Symbionts of Cnidaria: The psbA Minicircle of Symbiodinium
Pigment Signatures of the Phytoplankton Composition in the Northeastern Atlantic During the 1990 Spring Bloom
“Sea State: Recent Progress in the Context of Climate Change
In Coastal Ecosystems in Transition,” in A Comparative Analysis of the Northern Adriatic and Chesapeake Bay
Prevalent Endosymbiont Zonation Shapes the Depth Distributions of Scleractinian Coral Species
Symbiodinium Diversity in Mesophotic Coral Communities on the Great Barrier Reef: A First Assessment
Casado-Amezúa P.
New Insights Into the Genetic Diversity of Zooxanthellae in Mediterranean Anthozoans
CrossRef Full Text | Google Scholar
“Cassiopea and its zooxanthellae.” In The Cnidaria
Present and Future: The World of Medusa and Her Sisters (eBook)
(Switzerland: Springer International Publishing AG)
Google Scholar
Differences of Ammonia Metabolism in Symbiotic and Aposymbiotic Condylactus and Cassiopea Spp
CrossRef Full Text | Google Scholar
Environmental Populations of Symbiotic Dinoflagellates in the Genus Symbiodinium can Initiate Symbioses With Reef Cnidarians
Genetic Diversity of Symbiotic Dinoflagellates in the Genus Symbiodinium
PubMed Abstract | CrossRef Full Text | Google Scholar
Collins A. G., Jarms G., Morandini A. C. (2021) World List of Scyphozoa. Cotylorhiza Tuberculata (Macri 1778). Available at: https://www.marinespecies.org/aphia.php?p=taxdetails&id=135297on2021-12-26
Google Scholar
Understanding Diversity in Coral-Algal Symbiosis: A Cluster-Based Approach to Interpreting Fine-Scale Genetic Variation in the Genus Symbiodinium
CrossRef Full Text | Google Scholar
CrossRef Full Text | Google Scholar
CrossRef Full Text | Google Scholar
Enrique-Navarro A.
Living Inside a Jellyfish: The Symbiosis Case Study of Host-Specialized Dinoflagellates
and the Scyphozoan Cotylorhiza Tuberculata
Transmission Mode Predicts Specificity and Interaction Patterns in Coral-Symbiodinium Networks
Phyllorhiza Punctata Von Lendenfeld 1884 (Scyphozoa: Rhizostomeae: Mastigiidae) Reappeared Off the Mediterranean Coast of Israel
CrossRef Full Text | Google Scholar
The Scyphomedusae of the Mediterranean Coast of Israel
Including Two Lessepsian Migrants New to the Mediterranean
Google Scholar
Garcia-Cuetos L.
Molecular Evidence for Host–Symbiont Specificity in Soritid Foraminifera
Associations Within the Family Aiptasiidae
a Monophyletic Lineage of Symbiotic of Sea Anemones (Cnidaria
Effects of Island Mass: Water Flow and Plankton Pattern Around a Reef in the Great Barrier Reef Lagoon
CrossRef Full Text | Google Scholar
Nov.: A “Free-Living” Dinoflagellate From Tenerife (Northeast-Atlantic Ocean) 1
doi: 10.1111/j.1529-8817.2008.00621.x
Carbon Metabolism and Strobilation in Cassiopea Andromeda (Cnidaria: Scyphozoa): Significance of Endosymbiotic Dinoflagellates
CrossRef Full Text | Google Scholar
The Genetic and Physiological Characteristics of the Symbiodinium Spp
In the Endemic Anemone Anthopleura Aureoradiata
Australia: Victoria University of Wellington)
Google Scholar
Structure and Evolution of the rDNA Internal Transcribed Spacer (ITS) Region 2 in the Symbiotic Dinoflagellates (Symbiodinium
doi: 10.1111/j.1529-8817.2006.00309.x
MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability
Cotylorhiza Tuberculata (Cnidaria: Scyphozoa) - Life History of a Stationary Population.Mar
doi: 10.1111/j.1439-0485.1992.tb00359.x
CrossRef Full Text | Google Scholar
and Phylogeny of Endosymbiotic Dinoflagellates in the Genus Symbiodinium Using the ITS Region: In Search of a “Species” Level Marker
doi: 10.1046/j.1529-8817.2001.01031.x
Diversity and Community Structure of Symbiotic Dinoflagellates From Caribbean Coral Reefs
CrossRef Full Text | Google Scholar
PubMed Abstract | CrossRef Full Text | Google Scholar
Differ in Relative Dominance in Coral Reef Host Communities Across Environmental
Do Introduced Endosymbiotic Dinoflagellates “Take” to New Hosts
CrossRef Full Text | Google Scholar
Low Symbiont Diversity in Southern Great Barrier Reef Corals
Systematic Revision of Symbiodiniaceae Highlights the Antiquity and Diversity of Coral Endosymbionts
A Genetics–Based Description ofSymbiodinium Minutum Sp
Two Dinoflagellates Symbiotic With Cnidaria
doi: 10.1111/j.1529-8817.2012.01217.x
High Diversity and Host Specificity Observed Among Symbiotic Dinoflagellates in Reef Coral Communities From Hawaii
Revival of Philozoon Geddes for Host-Specialized Dinoflagellates
in Animals From Coastal Temperate Zones of Northern and Southern Hemispheres
““Cassiopea and its Zooxanthellae.”,” in The Cnidaria
Present and Future: The World of Medusa and Her Sisters
Google Scholar
a Dinoflagellate Common to Indo-Pacific Giant Clams
and a Revised Morphological Description of Symbiodinium Microadriaticum Freudenthal
Use of Antibiotics for Maintenance of Axenic Cultures of Amphidinium Carterae for the Analysis of Translation
CrossRef Full Text | Google Scholar
Diversity of Symbiodinium Dinoflagellate Symbionts From the Indo-Pacific Sea Slug Pteraeolidia Ianthina (Gastropoda: Mollusca)
High Photosynthetic Plasticity may Reinforce Invasiveness of Upside-Down Zooxanthellate Jellyfish in Mediterranean Coastal Waters
"The Rapid Determination of Algal Chlorophyll and Carotenoid Pigments and Their Breakdown Products in Natural Waters by Reverse-Phase High-Performance Liquid Chromatography."
Variation in Symbiont Uptake in the Early Ontogeny of the Upside-Down Jellyfish
Changes in Coral Microbial Communities in Response to a Natural pH Gradient
“On Dinoflagellate Phylogeny and Classification,” in Unravelling the Algae: The Past
CrossRef Full Text | Google Scholar
Acquisition and Proliferation of Algal Symbionts in Bleached Polyps of the Upside-Down Jellyfish
Upside-Down But Headed in the Right Direction: Review of the Highly VersatileCassiopea Xamachana System
Pérez-Ruzafa A.
Evidence of a Planktonic Food Web Response to Changes in Nutrient Input Dynamics in the Mar Menor Coastal Lagoon
Pestorić B.
Scyphomedusae and Ctenophora of the Eastern Adriatic: Historical Overview and New Data
Contrasting Contributions to Inorganic Nutrient Recycling by the Co-Occuring Jellyfishes
Catostylus Mosaicus and Phyllorhiza Punctata (Scyphozoa
A New Symbiodinium Clade (Dinophyceae) From Soritid Foraminifera in Hawai’i
Environmental Control of Phase Transition and Polyp Survival of a Massive-Outbreaker Jellyfish
Rambaut A. (2014) FigTree V1.4.2, A Graphical Viewer of Phylogenetic Trees. Available at: https://github.com/rambaut/figtree/releases
Google Scholar
Posterior Summarisation in Bayesian Phylogenetics Using Tracer 1.7
Raspor Dall’Olio L. (2016). Symbiosis Ecology of Selected Scyphozoa (Nova Gorica: Doctoral dissertation, University of Nova Gorica). Available at: https://repozitorij.ung.si/IzpisGradiva.php?id=2622&lang=eng&prip=rup:9058647:d4
Google Scholar
Rodriguez-Lanetty M.
Latitudinal Variability in Symbiont Specificity Within the Widespread Scleractinian Coral Plesiastrea Versipora
CrossRef Full Text | Google Scholar
MRBAYES 3: Bayesian Phylogenetic Inference Under Mixed Models
PubMed Abstract | CrossRef Full Text | Google Scholar
A Molecular Genetic Classification of Zooxanthellae and the Evolution of Animal-Algal Symbioses
A Shift to Parasitism in the Jellyfish Symbiont Symbiodinium Microadriaticum
doi: 10.1146/annurev.mi.31.100177.000543
Cohesive Molecular Genetic Data Delineate Species Diversity in the Dinoflagellate Genus Symbiodinium
doi: 10.1111/j.1365-294X.2008.04037.x
Genetic Comparisons of Freshly Isolated Versus Cultured Symbiotic Dinoflagellates: Implications for Extrapolating to the Intact Symbiosis
doi: 10.1046/j.1529-8817.2001.00194.x
Molecular Phylogeny of Symbiotic Dinoflagellates Inferred From Partial Chloroplast Large Subunit (23S)-rDNA Sequences
Molecular Diversity of Symbiotic Algae at the Latitudinal Margins of Their Distribution: Dinoflagellates of the GenusSymbiodinium in Corals and Sea Anemones
Specificity is Rarely Absolute in Coral-Algal Symbiosis: Implications for Coral Response to Climate Change
The Evolutionary History of Symbiodinium and Scleractinian Hosts—Symbiosis
Functional Diversity in Coral-Dinoflagellate Symbiosis
CrossRef Full Text | Google Scholar
Photosynthesis and Production of Hydrogen Peroxide by Symbiodinium (Pyrrhophyta) Phylotypes With Different Thermal Tolerances
doi: 10.1111/j.1529-8817.2008.00537.x
Natural Infections of Aposymbiotic Cassiopea Xamachana Scyphistomae From Environmental Pools of Symbiodinium
Correspondence Between Cold Tolerance and Temperate Biogeography in a Western Atlantic Symbiodinium (Dinophyta) Lineage
doi: 10.1111/j.1529-8817.2008.00567.x
Mode of Zooxanthella Transmission Does Not Affect Zooxanthella Diversity in Acroporid Corals
CrossRef Full Text | Google Scholar
CrossRef Full Text | Google Scholar
and Photophysiology of the Mangrove Jellyfish Cassiopea Xamachana Symbiotic With Zooxanthellae: Effect of Jellyfish Size and Season
Molecular Diversity of Symbiotic Algae of the Genus Symbiodinium (Zooxanthellae) in Cnidarians of the Mediterranean Sea
CrossRef Full Text | Google Scholar
DAMBE5: A Comprehensive Software Package for Data Analysis in Molecular Biology and Evolution
PubMed Abstract | CrossRef Full Text | Google Scholar
Revised Dinoflagellate Phylogeny Inferred From Molecular Analysis of Large-Subunit Ribosomal RNA Gene Sequences
Malej A and Ramšak A (2022) Diversity of Dinoflagellate Symbionts in Scyphozoan Hosts From Shallow Environments: The Mediterranean Sea and Cabo Frio (Rio de Janeiro
Received: 01 February 2022; Accepted: 12 April 2022;Published: 13 May 2022
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RIO DE JANEIRO, Brazil — Petrobras has completed a drillstem test (DST) on its recent 1-BRSA-1383A-RJS discovery (Alto de Cabo Frio Central Noroeste) in the presalt southern Campos Basin off Brazil
The find is 230 km offshore Rio de Janeiro in a water depth of 1,833 m
The DST assessed a thick interval of presalt carbonate reservoir rocks
Collected oil samples will undergo laboratory analyses
Petrobras credited the discovery on the strategy
to apply new solutions and to maximize the use of the data
including real-time processing during acquisition
The partners plan further appraisal of the accumulation
The Alto de Cabo Frio Central Block was awarded in October 2017
under the ANP’s third bidding round production sharing regime
Beachgoers congregate on Fort Beach in Cabo Frio
a cryptocurrency investment firm founded by Glaidson Acacio dos Santos
a former waiter-turned-multimillionaire who is the central figure in what is alleged to be one of Brazil’s biggest-ever pyramid schemes
was based in the beach town dos Santos called home
Bathers dive into the waters of Fort Beach in Cabo Frio
Brazil became one of the first countries in the world to approve Bitcoin exchange-traded funds
where some had seen their friends and neighbors reap rewards by investing their life savings in the cryptocurrency investment firm G.A.S Consulting & Technology
many began to fear the cost of missing out
Mayor Jose Bonifacio listens during an interview in his City Hall office of Cabo Frio
Bonifacio acknowledges his city found itself under a spell
“The talk of the town was to know how much [bitcoin] was at
A view of the luxury condominium where former waiter-turned-multimillionaire Glaidson Acacio dos Santos had a home in Cabo Frio
the founder of G.A.S Consulting & Technology
is the central figure in what is alleged to be one of Brazil’s biggest-ever pyramid schemes
an alleged victim of the G.A.S Consulting & Technology
walks off after an interview in Iguaba Grande
Federal and state police and prosecutors accuse the firm of running a sophisticated racket defrauding thousands of small-scale investors who believed they were getting rich off bitcoin’s steep appreciation
Do Carmo watched in horror as the seizures and arrests unfolded; he had invested the rest of his savings in the company just weeks earlier
As revenues rose for the cryptocurrency investment firm G.A.S Consulting & Technology
came to be known as the “New Egypt” and as the town’s top dog
dos Santos was the “Bitcoin Pharaoh.” (AP Photo/Bruna Prado)
during an interview in Sao Pedro da Aldeia
a lawyer representing victims of G.A.S Consulting & Technology
a cryptocurrency investment firm founded by a former waiter-turned-multimillionaire who has become the central figure in what is alleged to be one of Brazil’s biggest-ever pyramid schemes
Brazil’s federal police stormed the helipad of a boutique seaside hotel in Rio de Janeiro state
where they busted two men and a woman loading a chopper with 7 million reais ($1.3 million) in neatly packed bills
The detainees told police they worked for G.A.S
a cryptocurrency investment firm founded by a former waiter-turned-multimillionaire who is the central figure in what is alleged to be one of Brazil’s biggest-ever pyramid schemes
Police say the company owned by 38-year-old Glaidson Acácio dos Santos had total transactions worth at least $7 billion ($38 billion reais) from 2015 through mid-2021 as part of a Bitcoin-based Ponzi scheme that promised investors 10% monthly returns
In hundreds of pages of documents obtained by The Associated Press
federal and state police and prosecutors accuse dos Santos and his associates of running a sophisticated racket defrauding thousands of small-scale investors who believed they were getting rich off Bitcoin’s steep appreciation
He is now in a Rio jail awaiting trial on charges including racketeering
financial crimes and ordering the murder and attempted murder of two business competitors
He remains under investigation in the attempted murder of a third competitor
dos Santos has repeatedly asserted his innocence
His lawyers didn’t reply to AP requests for comment
dos Santos represents an unlikely hero to his fervent supporters
Many view him as a modest Black man whose unorthodox Bitcoin business made them wealthy by gaming a financial system they believe is rigged by wealthy white elites
The case also underscores the fast-growing appetite for cryptocurrencies in Brazil
where years of economic and political crises have made digital currencies an attractive shield against depreciation of the Brazilian real and double-digit inflation
Bitcoin fervor was particularly keen in Cabo Frio
A wave of cryptocurrency-related violence soon followed
Cabo Frio came to be known as the “New Egypt.” And as the town’s top dog
dos Santos was dubbed the “Bitcoin Pharaoh.”
Police say dos Santos began trading in Bitcoin after leaving his job as a waiter in 2014
A one-time evangelical preacher in training
he enlisted clients from the Universal Church of the Kingdom of God
who earned a referral fee for bringing in fresh recruits and kicking back money to G.A.S.
a cryptofinance researcher at Sao Paulo’s Getulio Vargas Foundation
said religious groups are often targeted by pyramid schemers
“It’s through contacts that you increase the base of the pyramid,” he said
the Universal Church said it was cooperating with authorities and accused dos Santos of “harassing and recruiting” pastors and their flocks to join his company
dos Santos was starting to make serious money — and attract authorities’ attention
That year his company’s transactions totaled nearly 10 million reais ($1.8 million)
15 times higher than the previous year as money siphoned in and out of his bank accounts from all over Brazil
The country’s financial intelligence unit also noticed the company — at the time registered as a restaurant — was regularly trading cryptocurrency on online exchange platforms
according to prosecutors: Dos Santos would instruct clients to deposit their money – in cash to avoid further scrutiny – into bank accounts run by managing partners
The money would then be transferred to dos Santos or his Venezuelan wife
use it to buy bitcoins and other cryptocurrencies as well as traditional financial assets
Clients were promised a 10% monthly return on their investments over 12- to 48-month contract periods
but did not own the bitcoins they were told G.A.S
it was risk-free: They would get their entire initial investment back at the end of the contract
dos Santos was fast becoming a celebrity in Cabo Frio
The chubby young man in thick-rimmed glasses was also gaining a taste for the high life
Dos Santos bought expensive jewelry and a swanky apartment as contracts poured in from elsewhere in Latin America and as far away as the U.S.
Brazil’s lenient laws regulating cryptocurrency helped fuel dos Santos’ rise
Brazil’s securities regulator was making digital currencies more attractive: It authorized the country’s investment funds to invest in cryptocurrencies in 2018
Brazil approved Bitcoin exchange-traded funds
only the second country in the world to do so
And Rio de Janeiro has recently said it wants to offer incentives to those paying city property taxes using bitcoins
trades in Brazilian reais on the world’s largest cryptocurrency exchange
jumped to nearly $8.5 billion in the fourth quarter of 2021
from just $152 million over the same period a year earlier
where residents had seen their neighbors reap rewards by investing their life savings in G.A.S.
After catching COVID-19 and struggling to get back to work
he was forced to tap his retirement savings to make ends meet
Then his therapist told him he sold his house to invest in G.A.S.
and had been receiving 10% monthly returns for a year
Do Carmo invested 40,000 reais ($7,000) — just over half the money left in his retirement fund
dos Santos’ success inspired other budding entrepreneurs to follow in his footsteps — not to mention those of Charles Ponzi
who died nearby in a Rio de Janeiro hospital charity ward in 1949
The Italian immigrant who engineered one of the largest scams in U.S
history in the 1920s was buried in a public Rio cemetery with his last $75
Some competitors promised even higher returns than G.A.S
Cabo Frio Mayor José Bonifácio acknowledged his city found itself under a spell
“The talk of the town was to know how much (Bitcoin) was at
he discussed with associates how rivals were encroaching on his turf
according to WhatsApp messages intercepted by federal police
I can’t let that happen,” dos Santos wrote
who advertised himself on social media as a cryptocurrency trader
Police accuse dos Santos of ordering the hit
Rio state police have also linked two attempted killings to dos Santos and what they called his “extermination team.” On March 20
a trader known as Nilsinho was shot while driving his BMW through Cabo Frio
Three months later another firm’s operator was targeted
his car hit by 40 bullets; he also survived
Things came to a head on April 28 when Rio federal police
seized the 7 million reais at the helipad of the Insolito Boutique Hotel in Buzios
A monthslong investigation into dos Santos’ business followed
alerted that dos Santos was planning to flee Brazil
federal police raided more than a dozen locations linked to G.A.S.
including dos Santos’ home where he was found with 13.8 million reais ($2.5 million) and taken into custody
Agents also found hard drives containing 10 times that amount in Bitcoin
including a white Porsche Panamera and an electric blue BMW Z4 convertible
Sixteen other associates were also charged
who left the country weeks before the raid and is believed to be in Florida
They say she withdrew more than 4,300 bitcoins worth $185 million (1 billion reais)
AP attempts to locate her were unsuccessful
your entire life wash away from one moment to the next.”
investors who had been receiving regular monthly payments refused to believe dos Santos did anything illegal
a crowd gathered outside broadcaster TV Globo in Rio de Janeiro to protest coverage of the alleged racket
scores of supporters blocked the street outside a federal courthouse in Rio
said the company offered an attractive alternative to a banking sector that “only charges you fees.”
offered investors a chance to “take part in the profit,” Brandão said
“‘Instead of giving you a crumb of the cake
he blamed the authorities for freezing G.A.S
assets and “prohibiting me from paying you.”
Brazilian law enforcement is still trying to uncover the true size of dos Santos’ empire
Prosecutors have identified at least 27,000 G.A.S
with operations in at least 13 Brazilian states and seven other countries
He said one of his clients enlisted her husband
investing a total 822,000 reais (about $150,000)
“It’s hard to have a conversation with anyone in Cabo Frio who doesn’t know someone who invested,” he said
Petrobras announced that it has discovered oil in the pre-salt in the Campos Basin’s southern portion
in a wildcat well within the Alto de Cabo Frio Central block
The well 1-BRSA-1383A-RJS (Alto de Cabo Frio Central Noroeste) is located 230 km from the city of Rio de Janeiro-RJ
The consortium will continue to drill to the final planned depth to evaluate the dimensions of the new accumulation and to characterize the quality of the fluids and reservoirs found
This discovery came as a result of a successful strategy of the consortium based on maximum use of data and on the application of new technological solutions in Big Data and Artificial Intelligence (AI)
Petrobras acquired the Alto de Cabo Frio Central block in October 2017
during the third bid round offered by the National Agency for Petroleum
with Pré-Sal Petróleo SA (PPSA) as manager
Petrobras has operatorship of the block with a 50% stake
in partnership with bp Energy do Brasil Ltda
Fatma Ahmed is a staff writer with six years’ experience in Journalism
She is working in the field of oil and gas for four years
She also worked in the field of economic journalism for 2 years
Fatma has a Bachelor Degree in Mass Communication
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2022 10h00 AM | Last Updated: November 01
“Negro entoou/ Um canto de revolta pelos ares/ No Quilombo dos Palmares/ Onde se refugiou” (A black man sang/ a revolt song out loud/ At Palmares Quilombo where he had found shelter”)
The iconic song entitled “Canto das Três Raças (Three Race Song),” by Mauro Duarte and Paulo César Pinheiro
in an unforgettable interpretation by Clara Nunes
ballroom of the Mario Joaquina Quilombo on August 17th
the community welcomed IBGE representatives
teams from institutions such as the Land and Cartography Institute of Rio de Janeiro State (ITERJ) and the National Coordination of Rural Quilombola Communities (Conaq)
and community residents on the Mobilization Day for the Quilombola Census
very close to the place called Armação de Búzios
and about 170 km far from the state capital
the quilombo was one of the sites chosen for the official opening of the nationwide quilombola census in 20220
On this ocassion the IBGE presented the methodology of the census operation in those areas in order to encourage participation by the quilombola population
the IBGE will have specific results for those territories
“That will be important for the establishment of public policies aimed at this population segment,” says the technical manager for the Census
The residents of such localities will have the opportunity to self-identify as quilombolas by answering the question “do you see yourself as a quilombola?”
which is part of the questionnaire adminstered in these communities only
the system will ask a second question: “What is the name of your community?” The 2022 Census application has a list of pre-registered communities
but it will be possible to add quilombola comunities not previously listed
Maria Joaquina expects to have a one-month long data collection period
Mixed-breed dogs playing in non-paved streets represent a simple landscape
but one that is marked by a history of fight and resistance
The quilombola community is part of a huge territory
which also encompasses the neighboring quilombolas of Rasa and Baía Formosa
It is believed that farm owners received slaves even after slave trade had been abolished
The territorial dispute dates back to the settlement period: the quilombolas claimed to have received tha area as a donation from Jesuit priests who were expelled from the place
The community was named Maria Joaquina after a slave trader
In “Social Cartography of QUIPEA (Quilombos in the Environmental Education Project)” which presents the history and geography of the community
the territory “incorporates the history of enslaved ancestors by adopting the name of an ancient slave trader
in order to represent resistance in their fight for freedom.”
IBGE enumerator in the community and a resident of the José Gonçalves neighborhood estimates that the data collection work for the 2022 in Maria Joaquina will last about a month
And he says he is happy for the warm welcoming of residents
“I have already eunmerated some housing units and they were very kind to me.”
The enumerators snd other members of the 2022 Census team who work in quilombola territories went through a specific day of training
besides the methodology adopted in questionnaires
Specific approach guides and a manual or the enumerator were also distributed to the agents who will work in these communities
In order to guarantee data collection quality
to speak with local communities and have a meeting with them to explain what the 2022 Census is
they request help from community leaders and memebers in order to collect data
enumerator João Gabriel will be helped by Clara Oliveira
a manicurist who will work as his guide in the locality
The work of a guide in the 2022 Census is to help in the enumerator’s safe visiting of all the housing units
the most appropriate times for visits and the behavior codes they should follow
“The community has open arms for the Census
main leader of the community and a coordinator for Conaq
adds: “The quilombo will welcome the Census with a sincerely open heart
It is so important for us to be enumerated!” During the vaccination against Covod-19 we had a lot of difficulty having access to the vaccine and in knowing how many we were.”
sanitation and health are reasons for maria Joaquina to eagerly await the survey results
“The Census will reveal our true story and evidence the lack of public policies here
It will show the portrait of a population that fights more and more for policies every day
And the real conditions of quilombola life in Brazil will be known,” Ms
She says the survey may help the quilombo obtain the awaited document granted by the National Institute for Colonization and Agrarian Reform (Incra)
Maria Joaquina has been recognized by Palmares Foundation
but its territorial status has not been regulated by Incra
The legal action started in 2013 and its latest update was in July 2021
Challenges to cartography in quilombola areas
One of the main challenges faced by the iBGE was to map the quilombola population
“Além dos territórios quilombolas oficialmente delimitados
informação que recebemos do Incra e dos institutos estaduais de terra
procuramos retratar também todas as comunidades quilombolas fora desses territórios e todas as localidades onde pode haver população quilombola residindo”
coordenadora do Censo em Povos e Comunidades Tradicionais
A total of 5,972 quilombolas localities and 2,308 settlements will be enumerated
They are part of 3,542 enumeration areas defined by the IBGE’s cartography department
The officially delimitated territories will answer
so that it is possible to present information as detailed as possible with data on education
and more information that is part of this questionnaire only
a geographer at eh and member of the Work Group of Traditional Communities and Peoples for the 2022 Census spoke about all the preparation phases for the census operation in quilombla areas
She highlights that the IBGE followed the WTO’ Convention 169
which deals with the necessity of traditional peoples being consulted before being subject to administrative measures that have a direct impact on their lives
“Consultation to local and regional leaders and to other bodies that work with quilombolas took place four times
Having a partnership with them was very important,” she adds
Vilanova says that both the construction of the question and all the work to be done counted on the help of other leaders
“And Maria Joaquina was one of the communities chosen for the rehearsal in 2017
It is a very important locality in this process.”
Conaq expects that the results released after the 2022 Census will strengthen quilombola communities in the process of granting rights to public policies
helping the institution obtain from institutions the inclusion of quilombolas in governmental practices
According to the executive director of Conaq
ministry or body has a different data set on the quilombola population
He hopes the 2022 Census will help unify these figures
“This proposal of obtaining information is extremely necessary because there is no high quality or enough amount of information on the quilombola population in Brazil
When the state acknowledges the existence of this population
undoubtedly that will be the basis for the elaboratio of public policies,” Mr
“The release of results will also be discussed in the post-census step
in order to reach the best formats to help the quilombola population.”
other communities took part in the Day o Mobilization of the Quilombola Census in the five Major Regions of Brazil: Muquém Quilombola Community (AL)
Forte Príncipe da Beira Quilombola Community (RO) Alpes Quilombola Territory (RS)
All the communities are being visited by enumerators wearing uniforms - an IBGE vest
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Cody Rego was a member of the North Run crew which had strong performances competing at the Tryon Fall 5
Rego and Cabo Frio DV Z won the Platinum Performance/USEF Talent Search 2 Class
During the WIHS Equitation Jumper Phase Class
the pair finished 4th competing in the WIHS Equitation Hunter Phase Class
and Rego and Cabo Frio DV Z finished third in the WIHS Overall Class
#Equestrian
Category: All, Sports
Documents from the Royal Society reveal the debate between State and Science, sparked by a shipwreck in Brazil
National Library of Australia Painting Death of a ship
This is the only known image of the shipNational Library of Australia
Royal Museums Greenwich Painter and sailor John Christian Schetky described, in Salvage of Stores and Treasures from HMS Thetis at Cape Frio, Brazil, 1833, the recovery of the treasure.Royal Museums Greenwich
Royal Museums Greenwich Captain DickinsonRoyal Museums Greenwich
Dickinson constructed two diving bells from water tanks he had taken from the ship
reinforcing them and installing glass windows to illuminate the interior
He also prepared an air pump to supply oxygen
given the backwardness of native workers,” the Royal Society document observes
The Thetis had sunk in the center of the inlet
Dickinson planned to string cables from one cliff to another in order to let the bell down
“The captain said he had tremendous difficulties because of the arduous nature of the work
exposure to the weather in their thatched huts
and the dangers of the dives into the sea—a combination of terrors that the author is convinced could be overcome only by English sailors,” the summary found at the British institution recalls
Dickinson also reports that the sailors had seen “five tigers on the beach.” Armed with rifles
the Englishmen fired into the shadows and found that these were sea-pigs
The “visit” by reptiles of frightening size
a man “who would never be afraid of foolish things,” but the serpent did indeed “unnerve the strongest of them,” the English commander wrote
It took several dives and a few deaths before the fortune in the Thetis could begin to be brought up from the bottom of the sea
They lived in huts in a settlement they dubbed “St
Thomas,” and where the captain fulfilled the obligations of an Englishman who worshiped his country
He celebrated dates such as the Battle of Trafalgar
This was one of the reasons why he had dismissed a group of Brazilians
the “half-breeds” who had joined him at the beginning of the salvage operation
the sailors invented codes for use between those who were on the bottom and those on the surface
so as to advise each other whether or not the captain was around
They worked 12-hour shifts without food or rest
They had to remove the detritus that covered the wreck
including bodies and spoiled food from the frigate
The toxic gas almost killed a group of salvagers
At the same time as Dickinson was using his bell in extremely rough seas
was hailing as “a marvel” a similar operation that he was conducting in Portsmouth,” Martins observes
The salvage of the Thetis was also one of the first occasions on which drawings of the seabed were made
more was invested in the story of its salvage than in the report of its loss
the event was a tribute to human perseverance in the presence of the devastating power of nature,” say Driver and Martins
“Imperial eyes saw in this procedure a more or less coherent network through which information circulated until finally it was translated into established knowledge,” the researchers observe
Royal Societya drawing of the diving bell drawn by his rival
“The State and scientists changed their focus from the colonial positions on land to the vast unexplored areas of the oceans
an intellectual space that teemed with commercial and imperial significance
they elevated the status of the recently-defined ‘scientist.’ Just as they regulated and manipulated the ocean on paper
the English Admiralty used the physical ocean to transport troops
and British culture to ends of the earth,” observes American historian Michael Reidy
author of Tides of History: Ocean Science and Her Majesty’s Navy (University of Chicago)
British naval domination was the result of close collaboration between the Admiralty and the scientific elite
they transformed the unclaimed immensity of the ocean into an organized network
the modern scientist: one of the important links in that connection
“Science broke down the limits of parsimonious support by the State in order to gain a much more generous and comprehensive financing for their research projects,” the historian explains
Cases like the Thetis forced the system to improve its network of knowledge and showed that when the subject was the sea
the more extensive the relationship between the State and science
The scientists involved in the imperial project knew that studies about the sea were very costly to finance and that only a powerful country like England was able to sustain it
“The ocean became the most fertile territory for research
with funding from the State and an international group of scientists
It was the interest in the ocean that turned science into a global task that depended heavily on support and participation by the government
This totally changed the way of conducting and thinking about science,” Reidy notes
The empire was subtly transformed by science; in turn
the modern scientist was molded by the military demand for intelligence and control over the oceans
“The interest in the fate of the Thetis on the part of the Royal Society fellows should be viewed in the context of those contemporaneous efforts to demonstrate the practical utility of scientific thought
There was no better example of that than the science of navigation,” says Driver
In his Preliminary Discourse on the Study of Natural Philosophy (1830)
astronomer John Hershel portrayed the ideal scientific observer as a well-trained naval officer
the route of a ship was like a kind of hypothesis
based on meticulous astronomical observations and mathematic calculations
that would be tested against the experience of a safe arrival at its destination
If the ship was the instrument of the experiment
its captain was the exemplary man of science
the ‘experience’ of navigating in the absence of points of reference failed
with catastrophic consequences for the captain and his crew
the attribution of cause and effect was inseparable from that of responsibility and blame,” Driver and Martins observe
it was necessary to explain what had happened in a scientific way
One of the answers was directly connected to a debate that had taken place in the 1820s and 1830s
when experts in the earth’s magnetism warned of the magnetic effects on ships’ compasses caused by the “local attraction.” “Iron ships were witnesses to the power that science played in the British domination of magnetic and oceanic currents
But the fate of that industry was at stake because of the navigational problems that arose with the use of iron in shipbuilding
because the hulls of ships caused changes in the compasses that left them rather unreliable,” says historian Alison Winter of the University of Chicago
author of Compasses All Awry: the Iron Ship and the Ambiguities of Cultural Authority in Victorian Britain
“When ships began to be lost because of their compasses
the absence of a reliable means of correcting them threatened to sink scientists’ credibility in the eyes of the public.” According to Winter
the subject of the disoriented compasses of lost ships was used to describe spiritual and intellectual uncertainty and the absence of clear
“The same magnetic forces used in navigation were used to portray the way leaders exercised their power,” the historian explains
The mixture of politics and science that dominated the Victorian period was already latent at the time of the Thetis
That explains why the Admiralty invested more than £ 500 in research by Peter Barlow
a professor of mathematics at the Royal Military Academy and member of the Royal Society
“every ship carries within it an insidious disease,” i.e.
In his 1831 exposition to the Royal Society
Barlow used as example “the sad wreck of His Majesty’s ship the Thetis,” to debate that “fundamental question” and suggested that it was the cause of the disaster
a large amount of iron had been used in its construction
“Since precautions were not taken to correct the distortions of the local attraction
I will not hesitate to state that this omission was sufficient to cause the accident,” he told the Royal Society audience
“If science can be introduced to facilitate progress in navigation and help make it safer
we cannot allow it to be disregarded in the British Navy,” Barlow added
The scientific community’s interest in the Thetis was not limited to the causes of the shipwreck
reports of Dickinson’s salvage operations were read at the Royal Society
his successor in dealing with the ship’s remains and the first to submit a report to the Society’s fellows in 1833
According to the summary available at the institution
“what was left of that poor ship was subjected to heavy pressure from the sea as if it were a hammer; the result was that a single mass was formed
“on one occasion we were visited by an enormous whale that came very close to the diving bell
The change of command happened against Dickinson’s will
as he found himself pushed aside after all his efforts
both men realized that the opportunistic order came from the commander of the British squadron in Rio
who wanted the laurels and profits for himself
This did not prevent a dispute from breaking out between them within the Royal Society while seeking recognition for the scientifically unprecedented salvage operation
Dickinson also complained that in addition to suffering physical infirmities
he had been forced to handle political issues in dealings with the Brazilians
“I was always afraid of the envy of the Brazilian government about our remaining on the island
I was accused of obstructing the activities of fishermen and later
the city authorities of Cabo Frio went to investigate what the group of Englishmen were doing at St
they were dumbfounded on seeing a town with comfortable houses
None of them spoke a word of English and after filling me with more ‘Your Excellencies’ than I could stand
they told me they had come to see with whether this was an invading force.” Dickinson
boasting of having learned Portuguese to such a point that he couldn’t be beat in terms of number of “Your Excellencies,” showed the officials his “fortification,” a term he used sarcastically
The Brazilians were frightened by what they took to be a cannon shot and the Englishman had fun describing his difficulty in making them understand that the noise came from the air jet of the diving bell’s pump
everyone drank to the health of William IV
the island had been a fishing station that had grown steadily since the 16th century
And so Dickinson’s remarks that it was thanks to the English that the town had grown are not valid
Nor is it surprising that a military force camped out for 18 months had worried the Brazilian government,” Driver and Martins observe
there was no reason to pay for lumber and other materials because everything on the island “was available and could not be considered anyone’s property,” reminders of the myths about tropical abundance
But they ended up having to pay rent for use of the space
a small price to pay for redemption of the failure implicit in the wreck of the Thetis
Although even today it is not known what caused the end of the frigate
it was on a Brazilian island that science was able to recoup the self esteem of the British naval empire
© Revista Pesquisa FAPESP - All rights reserved
Rio de Janeiro is looking for investors to join its new Sustainable Development Hub
which will sit in a 2 million sqm area near the local seaport and airport
São Paulo – The Economic Development Secretariat in Cabo Frio
Rio de Janeiro is seeking investors for its Sustainable Development Hub project
The goal is to diversify the economy away from tourism and royalties from oil exploration
but we want to move beyond that to create more jobs and development for the city,” the Secretariat’s Employment and Income superintendent João Marcello Neves told ANBA
Project design is expected to be completed by the end of the year
The allotted 2 million sqm area sits next to the Cabo Frio Airport and near Porto do Forno
the seaport in neighboring Arraial do Cabo
the Secretariat is seeking out investment abroad
which is reassuring when it comes to shuttling material in and out
We are open to any kinds of projects and we are willing to listen to any company that’s looking to join in
We do have a preference for sustainable economy
for projects that will not harm the environment
since this is an area of beaches and [environmental] reserves
adding that 90% of the hub’s area is still available
with the use of technologies to add value and increase output
as well as corporate skill-building programs and handicraft
The Cabo Frio City Hall is also planning to build a convention center in which to host trade shows and business events
The hub will be managed by the Cabo Frio Development Company (Codescaf)
a semi-public enterprise whose creation was approved this year
The City Hall retains a 51% stake,” Neves explained
Companies joining the Hub will get tax breaks
the City Hall may award land on a right-to-use basis
how many jobs and revenue they will generate
the Estaleiro Vera Cruz shipyard will repay us with a crafts school where 20 professionals will graduate each year
and most will be taken in by the shipyard itself,” he said
referring to one of the companies already slated to join the Hub
There is also a startup incubator and accelerator for ocean-related businesses
the initiative will get support from educational institutions including the Federal Universit of Rio de Janeiro (UFRJ)
to streamline the paperwork requirements involved in starting a business
so we will work with startups that relate to sustainable marine economics and technology
They will all work with these accredited educational institutions
which will act as incubators and accelerators
while the City Hall will facilitate the process of starting new businesses.”
For additional information please refer to the Cabo Frio Economic Development Secretariat website
The Gulf country has deposited its instrument of acceptance of the World Trade Organisation (WTO) Agreement on Fisheries Subsidies
which is aimed at curbing harmful subsidies that contribute to overfishing and promoting the sustainable management of global marine resources
The Brazil-Arab News Agency (ANBA) is the news website of the Arab Brazilian Chamber of Commerce
Its goal is to promote communication between Brazilians and Arabs.